AlfalfaGEDB | MS.gene068752

M. sativa cultivar XinJiangDaYe / MS.gene068752

KEGG: K02260
GO: GO:0000041 GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005758 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0016530 GO:0016531 GO:0030001 GO:0031970 GO:0031974 GO:0043167 GO:0043169 GO:0043233 GO:0046872 GO:0046914 GO:0051179 GO:0051234 GO:0070013 GO:0110165 GO:0140104
PFAM: PF05051.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068752.t1	XP_003591736.2	82.5	103	13	1	1	103	1	98	2.50E-19	104.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068752.t1	Q9LJQ9	88.6	44	5	0	60	103	31	74	4.8e-19	95.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068752.t1	G7I329	82.5	103	13	1	1	103	1	98	1.8e-19	104.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049140	MS.gene068752	0.801693	7.98E-49	-1.69E-46
MS.gene049756	MS.gene068752	0.803728	3.04E-49	-1.69E-46
MS.gene050277	MS.gene068752	0.818996	1.46E-52	-1.69E-46
MS.gene050303	MS.gene068752	0.836264	1.04E-56	-1.69E-46
MS.gene050317	MS.gene068752	0.823522	1.32E-53	-1.69E-46
MS.gene051245	MS.gene068752	0.805904	1.07E-49	-1.69E-46
MS.gene051452	MS.gene068752	0.826699	2.35E-54	-1.69E-46
MS.gene051676	MS.gene068752	0.806827	6.81E-50	-1.69E-46
MS.gene05188	MS.gene068752	0.813948	1.98E-51	-1.69E-46
MS.gene051903	MS.gene068752	0.815657	8.26E-52	-1.69E-46
MS.gene051975	MS.gene068752	0.847606	1.04E-59	-1.69E-46
MS.gene052302	MS.gene068752	0.806939	6.45E-50	-1.69E-46
MS.gene052376	MS.gene068752	0.813827	2.10E-51	-1.69E-46
MS.gene052434	MS.gene068752	0.812566	3.97E-51	-1.69E-46
MS.gene052954	MS.gene068752	0.811896	5.57E-51	-1.69E-46
MS.gene053058	MS.gene068752	0.809563	1.78E-50	-1.69E-46
MS.gene053109	MS.gene068752	0.807837	4.16E-50	-1.69E-46
MS.gene053298	MS.gene068752	0.816105	6.56E-52	-1.69E-46
MS.gene053963	MS.gene068752	0.806103	9.68E-50	-1.69E-46
MS.gene054657	MS.gene068752	0.805153	1.53E-49	-1.69E-46
MS.gene05469	MS.gene068752	0.824579	7.48E-54	-1.69E-46
MS.gene05517	MS.gene068752	0.820585	6.35E-53	-1.69E-46
MS.gene055441	MS.gene068752	0.807587	4.70E-50	-1.69E-46
MS.gene055603	MS.gene068752	0.818475	1.92E-52	-1.69E-46
MS.gene055623	MS.gene068752	0.805431	1.34E-49	-1.69E-46
MS.gene056403	MS.gene068752	0.815154	1.07E-51	-1.69E-46
MS.gene056605	MS.gene068752	0.825711	4.04E-54	-1.69E-46
MS.gene056631	MS.gene068752	0.823641	1.24E-53	-1.69E-46
MS.gene056787	MS.gene068752	0.815231	1.03E-51	-1.69E-46
MS.gene056986	MS.gene068752	0.867717	1.11E-65	-1.69E-46
MS.gene057055	MS.gene068752	0.816866	4.43E-52	-1.69E-46
MS.gene057079	MS.gene068752	0.820501	6.63E-53	-1.69E-46
MS.gene057094	MS.gene068752	0.8099	1.51E-50	-1.69E-46
MS.gene057140	MS.gene068752	0.820085	8.26E-53	-1.69E-46
MS.gene057234	MS.gene068752	0.805605	1.23E-49	-1.69E-46
MS.gene05781	MS.gene068752	0.813211	2.87E-51	-1.69E-46
MS.gene057999	MS.gene068752	0.84294	1.90E-58	-1.69E-46
MS.gene058126	MS.gene068752	0.803429	3.50E-49	-1.69E-46
MS.gene058238	MS.gene068752	0.861171	1.24E-63	-1.69E-46
MS.gene059360	MS.gene068752	0.824862	6.41E-54	-1.69E-46
MS.gene059830	MS.gene068752	0.959798	7.70E-118	-1.69E-46
MS.gene06001	MS.gene068752	0.800816	1.21E-48	-1.69E-46
MS.gene06010	MS.gene068752	0.806624	7.52E-50	-1.69E-46
MS.gene06011	MS.gene068752	0.854002	1.65E-61	-1.69E-46
MS.gene060297	MS.gene068752	0.835219	1.91E-56	-1.69E-46
MS.gene060572	MS.gene068752	0.808917	2.45E-50	-1.69E-46
MS.gene060586	MS.gene068752	0.860451	2.05E-63	-1.69E-46
MS.gene060763	MS.gene068752	0.800498	1.40E-48	-1.69E-46
MS.gene060830	MS.gene068752	0.807838	4.16E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068752.t1	MTR_1g092430	85.437	103	10	1	1	103	1	98	4.36e-45	141
MS.gene068752.t1	MTR_0271s0050	74.038	104	11	2	1	103	1	89	5.58e-39	125
MS.gene068752.t1	MTR_0648s0010	74.038	104	11	2	1	103	1	89	5.58e-39	125

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068752.t1	AT3G15352	88.889	45	5	59	103	53	97	6.22e-27	95.5
MS.gene068752.t1	AT3G15352	88.889	45	5	59	103	31	75	6.59e-27	94.7
MS.gene068752.t1	AT3G15352	88.889	45	5	59	103	30	74	7.31e-27	94.4
MS.gene068752.t1	AT3G15352	88.889	45	5	59	103	37	81	1.08e-26	94.4
MS.gene068752.t1	AT1G53030	82.222	45	8	59	103	28	72	1.65e-24	88.6

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGGCGTTGCTGCAGTTGC+TGG	0.322432	2.3:+18497317	None:intergenic
GCTGGCGTTGCTGCTGTTGA+TGG	0.363493	2.3:+18497290	None:intergenic
ACCAGCAACTGCAGCAACGC+CGG	0.403671	2.3:-18497318	MS.gene068752:CDS
AACGCCAGCGACGGCAGAAA+CGG	0.425755	2.3:-18497276	MS.gene068752:CDS
TCTCGCAGCCTCTTCGTGTC+AGG	0.437710	2.3:+18497188	None:intergenic
CAGTTGCTGGTGCTGTTGGT+TGG	0.439790	2.3:+18497330	None:intergenic
CGAATTTGTCTTCGTGCTGA+AGG	0.441593	2.3:-18497110	MS.gene068752:CDS
GCCGCCGTTTCTGCCGTCGC+TGG	0.445253	2.3:+18497272	None:intergenic
GCTGCTGTTGATGGCGCTGC+CGG	0.473729	2.3:+18497299	None:intergenic
GCTGCAGTTGCTGGTGCTGT+TGG	0.489129	2.3:+18497326	None:intergenic
AACAGCAGCAACGCCAGCGA+CGG	0.494709	2.3:-18497285	MS.gene068752:CDS
TTTGACACTTCACATGCTGC+TGG	0.518991	2.3:+18497245	None:intergenic
GCCAGCGACGGCAGAAACGG+CGG	0.552546	2.3:-18497273	MS.gene068752:CDS
TGGTTCGAAGCAGAGGAAGA+AGG	0.559341	2.3:+18497350	None:intergenic
TGTTGGTTGGTTCGAAGCAG+AGG	0.577421	2.3:+18497343	None:intergenic
GGTAACGCGCAAGTGCAAAA+CGG	0.577765	2.3:-18497383	MS.gene068752:CDS
ATGAGTGCATAGTGGAACAC+GGG	0.582328	2.3:-18497166	MS.gene068752:CDS
TCGATGAGCTTCAATCCACT+TGG	0.585020	2.3:+18497130	None:intergenic
GCGCTTGTCCTGACACGAAG+AGG	0.611666	2.3:-18497196	MS.gene068752:CDS
GCTGCGAGATGAGTGCATAG+TGG	0.621172	2.3:-18497174	MS.gene068752:CDS
GGGAGGATGCTTGTGCCAAG+TGG	0.626172	2.3:-18497145	MS.gene068752:CDS
GATGAGTGCATAGTGGAACA+CGG	0.674556	2.3:-18497167	MS.gene068752:CDS
GTGCATAGTGGAACACGGGG+AGG	0.723863	2.3:-18497162	MS.gene068752:CDS
TGAGTGCATAGTGGAACACG+GGG	0.769217	2.3:-18497165	MS.gene068752:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAACAGATTTTCTTCTTCTT+CGG	+	chr2.3:18497268-18497287	None:intergenic	30.0%
	CGAATTTGTCTTCGTGCTGA+AGG	-	chr2.3:18497373-18497392	MS.gene068752:CDS	45.0%
	TCGATGAGCTTCAATCCACT+TGG	+	chr2.3:18497356-18497375	None:intergenic	45.0%
	TTTGACACTTCACATGCTGC+TGG	+	chr2.3:18497241-18497260	None:intergenic	45.0%
!	ATGAGTGCATAGTGGAACAC+GGG	-	chr2.3:18497317-18497336	MS.gene068752:CDS	45.0%
!	GATGAGTGCATAGTGGAACA+CGG	-	chr2.3:18497316-18497335	MS.gene068752:CDS	45.0%
	GGTAACGCGCAAGTGCAAAA+CGG	-	chr2.3:18497100-18497119	MS.gene068752:CDS	50.0%
	TGGTTCGAAGCAGAGGAAGA+AGG	+	chr2.3:18497136-18497155	None:intergenic	50.0%
!	TGAGTGCATAGTGGAACACG+GGG	-	chr2.3:18497318-18497337	MS.gene068752:CDS	50.0%
!!	TGTTGGTTGGTTCGAAGCAG+AGG	+	chr2.3:18497143-18497162	None:intergenic	50.0%
	GCTGCGAGATGAGTGCATAG+TGG	-	chr2.3:18497309-18497328	MS.gene068752:CDS	55.0%
!!	CAGTTGCTGGTGCTGTTGGT+TGG	+	chr2.3:18497156-18497175	None:intergenic	55.0%
	AACAGCAGCAACGCCAGCGA+CGG	-	chr2.3:18497198-18497217	MS.gene068752:CDS	60.0%
	AACGCCAGCGACGGCAGAAA+CGG	-	chr2.3:18497207-18497226	MS.gene068752:CDS	60.0%
	ACCAGCAACTGCAGCAACGC+CGG	-	chr2.3:18497165-18497184	MS.gene068752:CDS	60.0%
	GCGCTTGTCCTGACACGAAG+AGG	-	chr2.3:18497287-18497306	MS.gene068752:CDS	60.0%
	GCTGGCGTTGCTGCTGTTGA+TGG	+	chr2.3:18497196-18497215	None:intergenic	60.0%
	GGGAGGATGCTTGTGCCAAG+TGG	-	chr2.3:18497338-18497357	MS.gene068752:CDS	60.0%
	TCTCGCAGCCTCTTCGTGTC+AGG	+	chr2.3:18497298-18497317	None:intergenic	60.0%
!	GTGCATAGTGGAACACGGGG+AGG	-	chr2.3:18497321-18497340	MS.gene068752:CDS	60.0%
!!	GCTGCAGTTGCTGGTGCTGT+TGG	+	chr2.3:18497160-18497179	None:intergenic	60.0%
!!	GCTGCTGTTGATGGCGCTGC+CGG	+	chr2.3:18497187-18497206	None:intergenic	65.0%
	GCCAGCGACGGCAGAAACGG+CGG	-	chr2.3:18497210-18497229	MS.gene068752:CDS	70.0%
	GCCGGCGTTGCTGCAGTTGC+TGG	+	chr2.3:18497169-18497188	None:intergenic	70.0%
	GCCGCCGTTTCTGCCGTCGC+TGG	+	chr2.3:18497214-18497233	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	18497097	18497408	18497097	ID=MS.gene068752
chr2.3	mRNA	18497097	18497408	18497097	ID=MS.gene068752.t1;Parent=MS.gene068752
chr2.3	exon	18497097	18497408	18497097	ID=MS.gene068752.t1.exon1;Parent=MS.gene068752.t1
chr2.3	CDS	18497097	18497408	18497097	ID=cds.MS.gene068752.t1;Parent=MS.gene068752.t1

Gene Sequence	>MS.gene068752 ATGGGTAACGCGCAAGTGCAAAACGGTGTTGCTGTTCCTTCTTCCTCTGCTTCGAACCAACCAACAGCACCAGCAACTGCAGCAACGCCGGCAGCGCCATCAACAGCAGCAACGCCAGCGACGGCAGAAACGGCGGCAACGCCAGCAGCATGTGAAGTGTCAAATGCTCCGAAGAAGAAGAAAATCTGTTGCGCTTGTCCTGACACGAAGAGGCTGCGAGATGAGTGCATAGTGGAACACGGGGAGGATGCTTGTGCCAAGTGGATTGAAGCTCATCGAATTTGTCTTCGTGCTGAAGGATTCAATGTTTGA
Protein sequence	>MS.gene068752.t1 MGNAQVQNGVAVPSSSASNQPTAPATAATPAAPSTAATPATAETAATPAACEVSNAPKKKKICCACPDTKRLRDECIVEHGEDACAKWIEAHRICLRAEGFNV