Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08582.t1 | XP_003606498.1 | 97.5 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 1.40E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08582.t1 | Q9M1E7 | 77.1 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 5.1e-49 | 194.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08582.t1 | G7JRH3 | 97.5 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 1.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene08582 | 0.800102 | 1.69E-48 | -1.69E-46 |
| MS.gene04919 | MS.gene08582 | 0.808359 | 3.22E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene08582 | 0.832085 | 1.16E-55 | -1.69E-46 |
| MS.gene049283 | MS.gene08582 | 0.872412 | 3.24E-67 | -1.69E-46 |
| MS.gene049499 | MS.gene08582 | 0.847246 | 1.31E-59 | -1.69E-46 |
| MS.gene049861 | MS.gene08582 | 0.812777 | 3.57E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene08582 | 0.816051 | 6.75E-52 | -1.69E-46 |
| MS.gene050300 | MS.gene08582 | -0.802556 | 5.30E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene08582 | 0.804157 | 2.47E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene08582 | 0.820506 | 6.62E-53 | -1.69E-46 |
| MS.gene051597 | MS.gene08582 | 0.832708 | 8.10E-56 | -1.69E-46 |
| MS.gene051699 | MS.gene08582 | 0.839962 | 1.16E-57 | -1.69E-46 |
| MS.gene051832 | MS.gene08582 | 0.822693 | 2.07E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene08582 | -0.821823 | 3.29E-53 | -1.69E-46 |
| MS.gene052686 | MS.gene08582 | 0.818076 | 2.37E-52 | -1.69E-46 |
| MS.gene052818 | MS.gene08582 | -0.808682 | 2.75E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene08582 | 0.806242 | 9.05E-50 | -1.69E-46 |
| MS.gene053295 | MS.gene08582 | 0.80657 | 7.71E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene08582 | 0.835119 | 2.02E-56 | -1.69E-46 |
| MS.gene053340 | MS.gene08582 | 0.811903 | 5.55E-51 | -1.69E-46 |
| MS.gene053456 | MS.gene08582 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene08582 | 0.804097 | 2.54E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene08582 | 0.875235 | 3.61E-68 | -1.69E-46 |
| MS.gene054222 | MS.gene08582 | 0.854228 | 1.42E-61 | -1.69E-46 |
| MS.gene054328 | MS.gene08582 | 0.806846 | 6.75E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene08582 | 0.813198 | 2.89E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene08582 | 0.805895 | 1.07E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene08582 | 0.827736 | 1.33E-54 | -1.69E-46 |
| MS.gene054893 | MS.gene08582 | 0.833834 | 4.25E-56 | -1.69E-46 |
| MS.gene054957 | MS.gene08582 | 0.829273 | 5.65E-55 | -1.69E-46 |
| MS.gene055724 | MS.gene08582 | 0.80082 | 1.20E-48 | -1.69E-46 |
| MS.gene056045 | MS.gene08582 | 0.803106 | 4.08E-49 | -1.69E-46 |
| MS.gene056403 | MS.gene08582 | 0.816682 | 4.88E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene08582 | 0.808095 | 3.67E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene08582 | 0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene08582 | 0.803883 | 2.82E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene08582 | 0.838371 | 2.99E-57 | -1.69E-46 |
| MS.gene057155 | MS.gene08582 | 0.828286 | 9.78E-55 | -1.69E-46 |
| MS.gene057622 | MS.gene08582 | 0.811798 | 5.85E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene08582 | 0.818926 | 1.52E-52 | -1.69E-46 |
| MS.gene057893 | MS.gene08582 | -0.813524 | 2.45E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene08582 | 0.823769 | 1.16E-53 | -1.69E-46 |
| MS.gene058159 | MS.gene08582 | 0.835646 | 1.49E-56 | -1.69E-46 |
| MS.gene058279 | MS.gene08582 | 0.816445 | 5.51E-52 | -1.69E-46 |
| MS.gene058592 | MS.gene08582 | 0.836845 | 7.37E-57 | -1.69E-46 |
| MS.gene05878 | MS.gene08582 | 0.828326 | 9.57E-55 | -1.69E-46 |
| MS.gene059146 | MS.gene08582 | 0.80805 | 3.75E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene08582 | 0.807749 | 4.34E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene08582 | 0.865847 | 4.39E-65 | -1.69E-46 |
| MS.gene05985 | MS.gene08582 | -0.838728 | 2.42E-57 | -1.69E-46 |
| MS.gene059901 | MS.gene08582 | -0.817035 | 4.06E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene08582 | 0.8361 | 1.14E-56 | -1.69E-46 |
| MS.gene06018 | MS.gene08582 | 0.847063 | 1.47E-59 | -1.69E-46 |
| MS.gene060365 | MS.gene08582 | 0.809016 | 2.33E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene08582 | 0.807935 | 3.96E-50 | -1.69E-46 |
| MS.gene060840 | MS.gene08582 | 0.809946 | 1.47E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene08582 | 0.804636 | 1.96E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene08582 | 0.835782 | 1.37E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene08582 | 0.820478 | 6.72E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene08582 | 0.84392 | 1.04E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene08582 | 0.811969 | 5.37E-51 | -1.69E-46 |
| MS.gene061188 | MS.gene08582 | 0.809076 | 2.26E-50 | -1.69E-46 |
| MS.gene061243 | MS.gene08582 | 0.802426 | 5.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08582.t1 | MTR_4g061010 | 97.458 | 118 | 3 | 0 | 1 | 118 | 54 | 171 | 3.73e-81 | 239 |
| MS.gene08582.t1 | MTR_6g033335 | 46.610 | 118 | 57 | 1 | 1 | 118 | 53 | 164 | 2.57e-31 | 112 |
| MS.gene08582.t1 | MTR_4g129670 | 47.458 | 118 | 60 | 1 | 1 | 118 | 53 | 168 | 3.35e-31 | 111 |
| MS.gene08582.t1 | MTR_1g095530 | 44.068 | 118 | 60 | 1 | 1 | 118 | 53 | 164 | 6.24e-23 | 90.5 |
| MS.gene08582.t1 | MTR_8g066790 | 39.474 | 114 | 65 | 1 | 5 | 118 | 57 | 166 | 7.92e-21 | 84.7 |
| MS.gene08582.t1 | MTR_8g097280 | 29.915 | 117 | 78 | 1 | 2 | 118 | 55 | 167 | 4.29e-18 | 77.8 |
| MS.gene08582.t1 | MTR_4g112570 | 36.735 | 98 | 62 | 0 | 1 | 98 | 56 | 153 | 3.11e-17 | 75.1 |
| MS.gene08582.t1 | MTR_8g101600 | 39.316 | 117 | 67 | 1 | 2 | 118 | 54 | 166 | 3.34e-16 | 72.4 |
| MS.gene08582.t1 | MTR_5g030070 | 40.367 | 109 | 61 | 1 | 10 | 118 | 62 | 166 | 3.31e-15 | 69.7 |
| MS.gene08582.t1 | MTR_2g056130 | 35.484 | 93 | 60 | 0 | 2 | 94 | 54 | 146 | 1.25e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08582.t1 | AT3G45600 | 77.119 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 2.06e-66 | 199 |
| MS.gene08582.t1 | AT3G45600 | 77.119 | 118 | 27 | 0 | 1 | 118 | 54 | 171 | 3.81e-65 | 199 |
| MS.gene08582.t1 | AT5G60220 | 65.217 | 115 | 40 | 0 | 4 | 118 | 57 | 171 | 1.04e-49 | 160 |
| MS.gene08582.t1 | AT2G23810 | 44.915 | 118 | 63 | 1 | 1 | 118 | 54 | 169 | 7.42e-30 | 108 |
| MS.gene08582.t1 | AT3G12090 | 44.915 | 118 | 59 | 2 | 1 | 118 | 53 | 164 | 8.88e-26 | 97.8 |
| MS.gene08582.t1 | AT4G30430 | 45.263 | 95 | 52 | 0 | 1 | 95 | 54 | 148 | 2.93e-24 | 93.6 |
| MS.gene08582.t1 | AT4G28050 | 38.983 | 118 | 70 | 1 | 1 | 118 | 54 | 169 | 1.89e-23 | 91.3 |
| MS.gene08582.t1 | AT5G46700 | 41.379 | 116 | 58 | 3 | 6 | 118 | 58 | 166 | 3.74e-21 | 85.5 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 2.95e-20 | 82.0 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 4.74e-20 | 81.6 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 9.74e-20 | 82.0 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 9.74e-20 | 82.0 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 1.13e-19 | 81.3 |
| MS.gene08582.t1 | AT1G63260 | 32.479 | 117 | 75 | 1 | 2 | 118 | 55 | 167 | 1.15e-19 | 81.6 |
| MS.gene08582.t1 | AT1G18520 | 37.607 | 117 | 70 | 2 | 2 | 118 | 56 | 169 | 3.52e-19 | 80.5 |
| MS.gene08582.t1 | AT2G19580 | 40.517 | 116 | 64 | 2 | 4 | 118 | 56 | 167 | 8.16e-17 | 73.9 |
| MS.gene08582.t1 | AT4G23410 | 34.783 | 115 | 69 | 1 | 4 | 118 | 56 | 164 | 1.74e-16 | 73.2 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTATGAATAGAGGGTATT+TGG | 0.185250 | 4.3:+41466881 | MS.gene08582:CDS |
| GCTTATGTTGTGACGGATAA+AGG | 0.213333 | 4.3:+41466846 | MS.gene08582:CDS |
| TTAGTCATTGGTGTGCTTAT+TGG | 0.213548 | 4.3:+41466810 | MS.gene08582:CDS |
| GGAAGATTAGTTCTTGTATA+AGG | 0.219711 | 4.3:+41466967 | MS.gene08582:CDS |
| TATTATTTGCAGGATTATTC+TGG | 0.279394 | 4.3:+41466906 | MS.gene08582:CDS |
| AGTGGCTAGTGATGAGTATT+GGG | 0.307047 | 4.3:+41466944 | MS.gene08582:CDS |
| AAGAAAACTCAATCCTATTC+AGG | 0.328328 | 4.3:+41467061 | MS.gene08582:CDS |
| GTATTTGGATTATTATTTGC+AGG | 0.348461 | 4.3:+41466896 | MS.gene08582:CDS |
| GTTGTCATGTTCTTAGTCAT+TGG | 0.349196 | 4.3:+41466798 | MS.gene08582:CDS |
| GAAGATTAGTTCTTGTATAA+GGG | 0.367420 | 4.3:+41466968 | MS.gene08582:CDS |
| ATATAAGAACATACCTGAAT+AGG | 0.397801 | 4.3:-41467074 | None:intergenic |
| CTTATGTTGTGACGGATAAA+GGG | 0.405211 | 4.3:+41466847 | MS.gene08582:CDS |
| GTAACACGCCCCTGCAAAAC+CGG | 0.415261 | 4.3:-41466745 | None:intergenic |
| ATCATGGTTGTTTCATTAGC+CGG | 0.418747 | 4.3:+41466726 | None:intergenic |
| CGGATAAAGGGTCTGGAAGA+AGG | 0.448561 | 4.3:+41466859 | MS.gene08582:CDS |
| TTCTAAAGCTTGTAGGAAGT+TGG | 0.451166 | 4.3:+41466992 | MS.gene08582:CDS |
| TAAGGGATTCTAAAGCTTGT+AGG | 0.452393 | 4.3:+41466985 | MS.gene08582:CDS |
| GAGTGGCTAGTGATGAGTAT+TGG | 0.454709 | 4.3:+41466943 | MS.gene08582:CDS |
| GGAAGAAGGGTTATGAATAG+AGG | 0.468654 | 4.3:+41466873 | MS.gene08582:CDS |
| GTTGTGACGGATAAAGGGTC+TGG | 0.489336 | 4.3:+41466852 | MS.gene08582:CDS |
| AAGAACATATCAGCAGTCTC+AGG | 0.515949 | 4.3:-41467035 | None:intergenic |
| ATTTGCAGGATTATTCTGGT+TGG | 0.516447 | 4.3:+41466910 | MS.gene08582:CDS |
| GCAGGATTATTCTGGTTGGT+TGG | 0.518544 | 4.3:+41466914 | MS.gene08582:CDS |
| AATATTTGCTTATGTTGTGA+CGG | 0.520715 | 4.3:+41466839 | MS.gene08582:CDS |
| GGATAAAGGGTCTGGAAGAA+GGG | 0.556945 | 4.3:+41466860 | MS.gene08582:CDS |
| GTGGCTAGTGATGAGTATTG+GGG | 0.574200 | 4.3:+41466945 | MS.gene08582:CDS |
| GAAGAAGGGTTATGAATAGA+GGG | 0.576688 | 4.3:+41466874 | MS.gene08582:CDS |
| AAGCTTGTAGGAAGTTGGCT+AGG | 0.591037 | 4.3:+41466997 | MS.gene08582:CDS |
| TGGTTGGTTGGAAGAGAGAG+TGG | 0.610133 | 4.3:+41466926 | MS.gene08582:CDS |
| TGGCTAGTGATGAGTATTGG+GGG | 0.638689 | 4.3:+41466946 | MS.gene08582:CDS |
| AACGTAACAAGAAAGTGTTA+CGG | 0.663996 | 4.3:-41466767 | None:intergenic |
| TGACTAAGAACATGACAACA+AGG | 0.676649 | 4.3:-41466794 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATATTTGCTTATGTTGTGA+CGG | + | chr4.3:41466839-41466858 | MS.gene08582:CDS | 25.0% |
| ! | GTATTTGGATTATTATTTGC+AGG | + | chr4.3:41466896-41466915 | MS.gene08582:CDS | 25.0% |
| ! | TATTATTTGCAGGATTATTC+TGG | + | chr4.3:41466906-41466925 | MS.gene08582:CDS | 25.0% |
| !! | GAAGATTAGTTCTTGTATAA+GGG | + | chr4.3:41466968-41466987 | MS.gene08582:CDS | 25.0% |
| ! | GGAAGATTAGTTCTTGTATA+AGG | + | chr4.3:41466967-41466986 | MS.gene08582:CDS | 30.0% |
| !! | AACGTAACAAGAAAGTGTTA+CGG | - | chr4.3:41466770-41466789 | None:intergenic | 30.0% |
| ATTTGCAGGATTATTCTGGT+TGG | + | chr4.3:41466910-41466929 | MS.gene08582:CDS | 35.0% | |
| GAAGAAGGGTTATGAATAGA+GGG | + | chr4.3:41466874-41466893 | MS.gene08582:CDS | 35.0% | |
| GTTGTCATGTTCTTAGTCAT+TGG | + | chr4.3:41466798-41466817 | MS.gene08582:CDS | 35.0% | |
| TGACTAAGAACATGACAACA+AGG | - | chr4.3:41466797-41466816 | None:intergenic | 35.0% | |
| TTAGTCATTGGTGTGCTTAT+TGG | + | chr4.3:41466810-41466829 | MS.gene08582:CDS | 35.0% | |
| !! | CTTATGTTGTGACGGATAAA+GGG | + | chr4.3:41466847-41466866 | MS.gene08582:CDS | 35.0% |
| !! | GGTTATGAATAGAGGGTATT+TGG | + | chr4.3:41466881-41466900 | MS.gene08582:CDS | 35.0% |
| !! | TAAGGGATTCTAAAGCTTGT+AGG | + | chr4.3:41466985-41467004 | MS.gene08582:CDS | 35.0% |
| !! | TTCTAAAGCTTGTAGGAAGT+TGG | + | chr4.3:41466992-41467011 | MS.gene08582:CDS | 35.0% |
| AAGAACATATCAGCAGTCTC+AGG | - | chr4.3:41467038-41467057 | None:intergenic | 40.0% | |
| AGTGGCTAGTGATGAGTATT+GGG | + | chr4.3:41466944-41466963 | MS.gene08582:CDS | 40.0% | |
| GGAAGAAGGGTTATGAATAG+AGG | + | chr4.3:41466873-41466892 | MS.gene08582:CDS | 40.0% | |
| !! | GCTTATGTTGTGACGGATAA+AGG | + | chr4.3:41466846-41466865 | MS.gene08582:CDS | 40.0% |
| !! | TTTCATTAGCCGGTTTTGCA+GGG | + | chr4.3:41466736-41466755 | MS.gene08582:CDS | 40.0% |
| AAGCTTGTAGGAAGTTGGCT+AGG | + | chr4.3:41466997-41467016 | MS.gene08582:CDS | 45.0% | |
| GAGTGGCTAGTGATGAGTAT+TGG | + | chr4.3:41466943-41466962 | MS.gene08582:CDS | 45.0% | |
| GGATAAAGGGTCTGGAAGAA+GGG | + | chr4.3:41466860-41466879 | MS.gene08582:CDS | 45.0% | |
| ! | GCAGGATTATTCTGGTTGGT+TGG | + | chr4.3:41466914-41466933 | MS.gene08582:CDS | 45.0% |
| ! | GTGGCTAGTGATGAGTATTG+GGG | + | chr4.3:41466945-41466964 | MS.gene08582:CDS | 45.0% |
| !! | GTTTCATTAGCCGGTTTTGC+AGG | + | chr4.3:41466735-41466754 | MS.gene08582:CDS | 45.0% |
| !! | TGGCTAGTGATGAGTATTGG+GGG | + | chr4.3:41466946-41466965 | MS.gene08582:CDS | 45.0% |
| !! | TTCATTAGCCGGTTTTGCAG+GGG | + | chr4.3:41466737-41466756 | MS.gene08582:CDS | 45.0% |
| CGGATAAAGGGTCTGGAAGA+AGG | + | chr4.3:41466859-41466878 | MS.gene08582:CDS | 50.0% | |
| ! | TGGTTGGTTGGAAGAGAGAG+TGG | + | chr4.3:41466926-41466945 | MS.gene08582:CDS | 50.0% |
| !! | GTTGTGACGGATAAAGGGTC+TGG | + | chr4.3:41466852-41466871 | MS.gene08582:CDS | 50.0% |
| GTAACACGCCCCTGCAAAAC+CGG | - | chr4.3:41466748-41466767 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 41466729 | 41467082 | 41466729 | ID=MS.gene08582 |
| chr4.3 | mRNA | 41466729 | 41467082 | 41466729 | ID=MS.gene08582.t1;Parent=MS.gene08582 |
| chr4.3 | exon | 41466729 | 41467082 | 41466729 | ID=MS.gene08582.t1.exon1;Parent=MS.gene08582.t1 |
| chr4.3 | CDS | 41466729 | 41467082 | 41466729 | ID=cds.MS.gene08582.t1;Parent=MS.gene08582.t1 |
| Gene Sequence |
| Protein sequence |
