AlfalfaGEDB | MS.gene20430

M. sativa cultivar XinJiangDaYe / MS.gene20430

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20430.t1	AES74719.2	99.2	131	1	0	1	131	85	215	9.80E-69	269.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20430.t1	P0C8Q9	51.5	99	45	2	33	131	150	245	1.6e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20430.t1	G7KKU8	99.2	131	1	0	1	131	85	215	7.1e-69	269.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene20430	0.821422	4.07E-53	-1.69E-46
MS.gene049378	MS.gene20430	0.802086	6.63E-49	-1.69E-46
MS.gene049419	MS.gene20430	-0.815025	1.14E-51	-1.69E-46
MS.gene049653	MS.gene20430	0.813369	2.65E-51	-1.69E-46
MS.gene050105	MS.gene20430	0.801216	1.00E-48	-1.69E-46
MS.gene050312	MS.gene20430	0.813809	2.12E-51	-1.69E-46
MS.gene050953	MS.gene20430	0.808685	2.74E-50	-1.69E-46
MS.gene051452	MS.gene20430	0.86788	9.87E-66	-1.69E-46
MS.gene051975	MS.gene20430	0.811113	8.24E-51	-1.69E-46
MS.gene052176	MS.gene20430	0.823623	1.25E-53	-1.69E-46
MS.gene052302	MS.gene20430	0.800138	1.66E-48	-1.69E-46
MS.gene052376	MS.gene20430	0.811194	7.91E-51	-1.69E-46
MS.gene052426	MS.gene20430	0.803008	4.28E-49	-1.69E-46
MS.gene052500	MS.gene20430	0.825154	5.47E-54	-1.69E-46
MS.gene052502	MS.gene20430	0.819823	9.49E-53	-1.69E-46
MS.gene052824	MS.gene20430	0.821931	3.11E-53	-1.69E-46
MS.gene052826	MS.gene20430	0.819001	1.46E-52	-1.69E-46
MS.gene052954	MS.gene20430	0.857384	1.69E-62	-1.69E-46
MS.gene053006	MS.gene20430	0.830638	2.62E-55	-1.69E-46
MS.gene053094	MS.gene20430	0.803994	2.67E-49	-1.69E-46
MS.gene053188	MS.gene20430	0.806269	8.93E-50	-1.69E-46
MS.gene053216	MS.gene20430	0.820891	5.40E-53	-1.69E-46
MS.gene053340	MS.gene20430	0.835666	1.47E-56	-1.69E-46
MS.gene053963	MS.gene20430	0.838865	2.23E-57	-1.69E-46
MS.gene054479	MS.gene20430	0.806477	8.07E-50	-1.69E-46
MS.gene054657	MS.gene20430	0.818361	2.04E-52	-1.69E-46
MS.gene05469	MS.gene20430	0.825913	3.62E-54	-1.69E-46
MS.gene05510	MS.gene20430	0.829941	3.88E-55	-1.69E-46
MS.gene055603	MS.gene20430	0.807509	4.88E-50	-1.69E-46
MS.gene055623	MS.gene20430	0.840267	9.64E-58	-1.69E-46
MS.gene055701	MS.gene20430	0.829904	3.96E-55	-1.69E-46
MS.gene055841	MS.gene20430	0.806953	6.40E-50	-1.69E-46
MS.gene056506	MS.gene20430	0.821343	4.25E-53	-1.69E-46
MS.gene056986	MS.gene20430	0.83359	4.89E-56	-1.69E-46
MS.gene057026	MS.gene20430	0.821544	3.82E-53	-1.69E-46
MS.gene057079	MS.gene20430	0.827296	1.69E-54	-1.69E-46
MS.gene057208	MS.gene20430	0.835299	1.82E-56	-1.69E-46
MS.gene057392	MS.gene20430	0.829538	4.87E-55	-1.69E-46
MS.gene05795	MS.gene20430	0.833654	4.71E-56	-1.69E-46
MS.gene057999	MS.gene20430	0.810502	1.12E-50	-1.69E-46
MS.gene058009	MS.gene20430	0.80103	1.09E-48	-1.69E-46
MS.gene058238	MS.gene20430	0.827139	1.85E-54	-1.69E-46
MS.gene058498	MS.gene20430	0.829085	6.27E-55	-1.69E-46
MS.gene058500	MS.gene20430	0.818233	2.18E-52	-1.69E-46
MS.gene058595	MS.gene20430	0.802021	6.84E-49	-1.69E-46
MS.gene058979	MS.gene20430	0.807024	6.18E-50	-1.69E-46
MS.gene059317	MS.gene20430	0.818807	1.62E-52	-1.69E-46
MS.gene059360	MS.gene20430	0.806307	8.76E-50	-1.69E-46
MS.gene059710	MS.gene20430	0.815775	7.78E-52	-1.69E-46
MS.gene059830	MS.gene20430	0.836745	7.82E-57	-1.69E-46
MS.gene059952	MS.gene20430	0.802461	5.55E-49	-1.69E-46
MS.gene060297	MS.gene20430	0.836181	1.09E-56	-1.69E-46
MS.gene060572	MS.gene20430	0.822018	2.96E-53	-1.69E-46
MS.gene060586	MS.gene20430	0.842116	3.15E-58	-1.69E-46
MS.gene060879	MS.gene20430	0.825463	4.62E-54	-1.69E-46
MS.gene060935	MS.gene20430	0.830991	2.15E-55	-1.69E-46
MS.gene060937	MS.gene20430	0.828377	9.30E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20430.t1	MTR_6g012390	99.237	131	1	0	1	131	85	215	1.52e-93	269
MS.gene20430.t1	MTR_7g010930	77.692	130	28	1	2	131	89	217	1.70e-62	190
MS.gene20430.t1	MTR_2g021720	39.516	124	62	4	8	128	79	192	3.83e-15	68.9
MS.gene20430.t1	MTR_2g021720	39.516	124	62	4	8	128	79	192	5.21e-15	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20430.t1	AT3G01170	63.359	131	39	2	2	131	93	215	1.03e-53	168
MS.gene20430.t1	AT5G15260	66.154	130	35	4	8	131	108	234	2.64e-53	167
MS.gene20430.t1	AT5G15260	66.154	130	35	4	8	131	108	234	2.64e-53	167
MS.gene20430.t1	AT5G19025	51.515	99	45	2	33	131	150	245	5.64e-23	90.5
MS.gene20430.t1	AT3G06180	53.535	99	43	2	33	131	132	227	7.07e-23	90.1
MS.gene20430.t1	AT5G19025	50.526	95	45	1	33	127	150	242	1.06e-22	90.1

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTATATTTATATATCTAT+TGG	0.238022	6.3:-6879583	MS.gene20430:CDS
TGTTGGTAGGTTAGAAGTTC+CGG	0.289963	6.3:-6879266	MS.gene20430:CDS
AAGAAGATGGCACCGATTAA+TGG	0.325801	6.3:-6879328	MS.gene20430:CDS
CCAATAGATATATAAATATA+AGG	0.341548	6.3:+6879583	None:intergenic
TTTGTGCTGCATTGGCTACA+TGG	0.387252	6.3:-6879549	MS.gene20430:CDS
TTCTTCAAACCCTTACAATT+CGG	0.389737	6.3:+6879490	None:intergenic
CGAAGTTTCTTCGTCGGTCC+CGG	0.401109	6.3:+6879247	None:intergenic
GTTGGTAGGTTAGAAGTTCC+GGG	0.404512	6.3:-6879265	MS.gene20430:CDS
ATGTGGTAATCCGAATTGTA+AGG	0.420695	6.3:-6879500	MS.gene20430:CDS
GATGGCGGTGTTTGTAAGAA+GGG	0.429289	6.3:-6879400	MS.gene20430:CDS
AGCACAGCTCTTCCATTAAT+CGG	0.433317	6.3:+6879316	None:intergenic
AGATGGCGGTGTTTGTAAGA+AGG	0.445969	6.3:-6879401	MS.gene20430:CDS
CTGTGCTTGTGCTGAGAGCT+AGG	0.461752	6.3:-6879300	MS.gene20430:CDS
GGTGTGGTTGTTCTGTTGGT+AGG	0.475981	6.3:-6879279	MS.gene20430:CDS
GCTAGGTGTGGTTGTTCTGT+TGG	0.477365	6.3:-6879283	MS.gene20430:CDS
ATCTTCCGAAGTTTCTTCGT+CGG	0.488611	6.3:+6879241	None:intergenic
TTGTGCTGCATTGGCTACAT+GGG	0.507240	6.3:-6879548	MS.gene20430:CDS
AAGTGTTGTTTGTGCTGCAT+TGG	0.523765	6.3:-6879557	MS.gene20430:CDS
ACTTCGGAAGATCAAGAAGT+AGG	0.555452	6.3:-6879230	None:intergenic
TGTGGTAATCCGAATTGTAA+GGG	0.569597	6.3:-6879499	MS.gene20430:CDS
TGAAGCGGAGCTTAAGAAGA+TGG	0.571103	6.3:-6879341	MS.gene20430:CDS
ATTGCCGCGTGATCATCATA+AGG	0.580739	6.3:-6879368	MS.gene20430:CDS
CCGCCATCTTTCACAAAAGA+CGG	0.590770	6.3:+6879415	None:intergenic
GTTTGCACAAGTAACAAATG+TGG	0.622560	6.3:-6879517	MS.gene20430:CDS
TCATCATAAGGAACTTGAAG+CGG	0.636755	6.3:-6879356	MS.gene20430:CDS
CGGGACCGACGAAGAAACTT+CGG	0.636873	6.3:-6879246	MS.gene20430:CDS
TGTGCTGCATTGGCTACATG+GGG	0.654737	6.3:-6879547	MS.gene20430:CDS
CTTGTGCTGAGAGCTAGGTG+TGG	0.697858	6.3:-6879295	MS.gene20430:CDS
AGTTCCTTATGATGATCACG+CGG	0.724998	6.3:+6879364	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CCAATAGATATATAAATATA+AGG	+	chr6.3:6879255-6879274	None:intergenic	15.0%
!!	CCTTATATTTATATATCTAT+TGG	-	chr6.3:6879252-6879271	MS.gene20430:CDS	15.0%
	TTCTTCAAACCCTTACAATT+CGG	+	chr6.3:6879348-6879367	None:intergenic	30.0%
	ATGTGGTAATCCGAATTGTA+AGG	-	chr6.3:6879335-6879354	MS.gene20430:CDS	35.0%
	GTTTGCACAAGTAACAAATG+TGG	-	chr6.3:6879318-6879337	MS.gene20430:CDS	35.0%
	TCATCATAAGGAACTTGAAG+CGG	-	chr6.3:6879479-6879498	MS.gene20430:CDS	35.0%
	TGTGGTAATCCGAATTGTAA+GGG	-	chr6.3:6879336-6879355	MS.gene20430:CDS	35.0%
	AGCACAGCTCTTCCATTAAT+CGG	+	chr6.3:6879522-6879541	None:intergenic	40.0%
	AGTTCCTTATGATGATCACG+CGG	+	chr6.3:6879474-6879493	None:intergenic	40.0%
	ATCTTCCGAAGTTTCTTCGT+CGG	+	chr6.3:6879597-6879616	None:intergenic	40.0%
!	TCACCGTCTTTTGTGAAAGA+TGG	-	chr6.3:6879417-6879436	MS.gene20430:CDS	40.0%
!	TGTTGGTAGGTTAGAAGTTC+CGG	-	chr6.3:6879569-6879588	MS.gene20430:CDS	40.0%
!!	AAGAAGATGGCACCGATTAA+TGG	-	chr6.3:6879507-6879526	MS.gene20430:CDS	40.0%
!!	AAGTGTTGTTTGTGCTGCAT+TGG	-	chr6.3:6879278-6879297	MS.gene20430:CDS	40.0%
	ATTGCCGCGTGATCATCATA+AGG	-	chr6.3:6879467-6879486	MS.gene20430:CDS	45.0%
	CCGCCATCTTTCACAAAAGA+CGG	+	chr6.3:6879423-6879442	None:intergenic	45.0%
	TGAAGCGGAGCTTAAGAAGA+TGG	-	chr6.3:6879494-6879513	MS.gene20430:CDS	45.0%
!	AGATGGCGGTGTTTGTAAGA+AGG	-	chr6.3:6879434-6879453	MS.gene20430:CDS	45.0%
!	CCGTCTTTTGTGAAAGATGG+CGG	-	chr6.3:6879420-6879439	MS.gene20430:CDS	45.0%
!	GATGGCGGTGTTTGTAAGAA+GGG	-	chr6.3:6879435-6879454	MS.gene20430:CDS	45.0%
!	GTTGGTAGGTTAGAAGTTCC+GGG	-	chr6.3:6879570-6879589	MS.gene20430:CDS	45.0%
!!	TTGTGCTGCATTGGCTACAT+GGG	-	chr6.3:6879287-6879306	MS.gene20430:CDS	45.0%
!!	TTTGTGCTGCATTGGCTACA+TGG	-	chr6.3:6879286-6879305	MS.gene20430:CDS	45.0%
	GCTAGGTGTGGTTGTTCTGT+TGG	-	chr6.3:6879552-6879571	MS.gene20430:CDS	50.0%
	GGTGTGGTTGTTCTGTTGGT+AGG	-	chr6.3:6879556-6879575	MS.gene20430:CDS	50.0%
!!	TGTGCTGCATTGGCTACATG+GGG	-	chr6.3:6879288-6879307	MS.gene20430:CDS	50.0%
	CGAAGTTTCTTCGTCGGTCC+CGG	+	chr6.3:6879591-6879610	None:intergenic	55.0%
	CGGGACCGACGAAGAAACTT+CGG	-	chr6.3:6879589-6879608	MS.gene20430:CDS	55.0%
	CTGTGCTTGTGCTGAGAGCT+AGG	-	chr6.3:6879535-6879554	MS.gene20430:CDS	55.0%
	CTTGTGCTGAGAGCTAGGTG+TGG	-	chr6.3:6879540-6879559	MS.gene20430:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	6879231	6879626	6879231	ID=MS.gene20430
chr6.3	mRNA	6879231	6879626	6879231	ID=MS.gene20430.t1;Parent=MS.gene20430
chr6.3	exon	6879231	6879626	6879231	ID=MS.gene20430.t1.exon1;Parent=MS.gene20430.t1
chr6.3	CDS	6879231	6879626	6879231	ID=cds.MS.gene20430.t1;Parent=MS.gene20430.t1

Gene Sequence

>MS.gene20430

ATGGAAGAAGTTTCTTATGCACCTTATATTTATATATCTATTGGAATAAGTGTTGTTTGTGCTGCATTGGCTACATGGGGTGTTATTGTTTGCACAAGTAACAAATGTGGTAATCCGAATTGTAAGGGTTTGAAGAAAGCTGCAGAATTTGATATTCAGTTAGAGACTGAAGATTGTGTGAAGAATTCACCGTCTTTTGTGAAAGATGGCGGTGTTTGTAAGAAGGGTTTGTTTGAATTGCCGCGTGATCATCATAAGGAACTTGAAGCGGAGCTTAAGAAGATGGCACCGATTAATGGAAGAGCTGTGCTTGTGCTGAGAGCTAGGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGACCGACGAAGAAACTTCGGAAGATCAAGAAGTAG

Protein sequence

>MS.gene20430.t1

MEEVSYAPYIYISIGISVVCAALATWGVIVCTSNKCGNPNCKGLKKAAEFDIQLETEDCVKNSPSFVKDGGVCKKGLFELPRDHHKELEAELKKMAPINGRAVLVLRARCGCSVGRLEVPGPTKKLRKIKK