AlfalfaGEDB | MS.gene23304

M. sativa cultivar XinJiangDaYe / MS.gene23304

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23304.t1	PNY08254.1	71.7	226	48	6	1	212	1	224	9.30E-69	270

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23304.t1	A0A2K3NYY7	71.7	226	48	6	1	212	1	224	6.7e-69	270.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene23304	0.820695	5.99E-53	-1.69E-46
MS.gene049404	MS.gene23304	0.823466	1.36E-53	-1.69E-46
MS.gene049669	MS.gene23304	0.820478	6.71E-53	-1.69E-46
MS.gene050119	MS.gene23304	0.802647	5.08E-49	-1.69E-46
MS.gene050228	MS.gene23304	0.803224	3.86E-49	-1.69E-46
MS.gene050303	MS.gene23304	0.81569	8.12E-52	-1.69E-46
MS.gene050355	MS.gene23304	0.817593	3.04E-52	-1.69E-46
MS.gene050605	MS.gene23304	0.800202	1.61E-48	-1.69E-46
MS.gene050680	MS.gene23304	0.834536	2.83E-56	-1.69E-46
MS.gene050750	MS.gene23304	0.801121	1.05E-48	-1.69E-46
MS.gene050780	MS.gene23304	0.830498	2.84E-55	-1.69E-46
MS.gene050807	MS.gene23304	0.829375	5.33E-55	-1.69E-46
MS.gene050844	MS.gene23304	0.804053	2.60E-49	-1.69E-46
MS.gene050953	MS.gene23304	0.810355	1.20E-50	-1.69E-46
MS.gene051265	MS.gene23304	0.807347	5.28E-50	-1.69E-46
MS.gene052176	MS.gene23304	0.810598	1.06E-50	-1.69E-46
MS.gene052190	MS.gene23304	0.807406	5.13E-50	-1.69E-46
MS.gene052208	MS.gene23304	0.802766	4.80E-49	-1.69E-46
MS.gene052286	MS.gene23304	0.819576	1.08E-52	-1.69E-46
MS.gene052302	MS.gene23304	0.806619	7.53E-50	-1.69E-46
MS.gene052376	MS.gene23304	0.819051	1.42E-52	-1.69E-46
MS.gene052426	MS.gene23304	0.805081	1.59E-49	-1.69E-46
MS.gene052867	MS.gene23304	0.802468	5.53E-49	-1.69E-46
MS.gene053006	MS.gene23304	0.823375	1.43E-53	-1.69E-46
MS.gene053080	MS.gene23304	0.801078	1.07E-48	-1.69E-46
MS.gene053166	MS.gene23304	0.825559	4.39E-54	-1.69E-46
MS.gene053953	MS.gene23304	0.807767	4.30E-50	-1.69E-46
MS.gene054405	MS.gene23304	0.803873	2.83E-49	-1.69E-46
MS.gene054537	MS.gene23304	0.801246	9.86E-49	-1.69E-46
MS.gene054559	MS.gene23304	0.809742	1.63E-50	-1.69E-46
MS.gene054657	MS.gene23304	0.819093	1.39E-52	-1.69E-46
MS.gene054994	MS.gene23304	0.82065	6.13E-53	-1.69E-46
MS.gene05510	MS.gene23304	0.825026	5.87E-54	-1.69E-46
MS.gene055356	MS.gene23304	0.814843	1.25E-51	-1.69E-46
MS.gene055357	MS.gene23304	0.806242	9.05E-50	-1.69E-46
MS.gene055358	MS.gene23304	0.819866	9.27E-53	-1.69E-46
MS.gene055608	MS.gene23304	0.801514	8.69E-49	-1.69E-46
MS.gene055701	MS.gene23304	0.833091	6.51E-56	-1.69E-46
MS.gene055818	MS.gene23304	0.81825	2.16E-52	-1.69E-46
MS.gene056498	MS.gene23304	0.80188	7.31E-49	-1.69E-46
MS.gene056506	MS.gene23304	0.823385	1.43E-53	-1.69E-46
MS.gene057026	MS.gene23304	0.808428	3.11E-50	-1.69E-46
MS.gene057060	MS.gene23304	0.866228	3.32E-65	-1.69E-46
MS.gene057555	MS.gene23304	0.806014	1.01E-49	-1.69E-46
MS.gene057639	MS.gene23304	0.802821	4.68E-49	-1.69E-46
MS.gene057693	MS.gene23304	0.842668	2.25E-58	-1.69E-46
MS.gene057869	MS.gene23304	0.816304	5.92E-52	-1.69E-46
MS.gene05795	MS.gene23304	0.826658	2.40E-54	-1.69E-46
MS.gene058548	MS.gene23304	0.812505	4.10E-51	-1.69E-46
MS.gene058979	MS.gene23304	0.80998	1.45E-50	-1.69E-46
MS.gene059128	MS.gene23304	0.815329	9.78E-52	-1.69E-46
MS.gene059317	MS.gene23304	0.840832	6.85E-58	-1.69E-46
MS.gene05954	MS.gene23304	0.806278	8.89E-50	-1.69E-46
MS.gene059607	MS.gene23304	0.804224	2.39E-49	-1.69E-46
MS.gene060310	MS.gene23304	0.809439	1.89E-50	-1.69E-46
MS.gene060322	MS.gene23304	0.825647	4.18E-54	-1.69E-46
MS.gene061040	MS.gene23304	-0.818489	1.91E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23304.t1	MTR_4g070630	86.310	168	22	1	46	212	1	168	8.15e-100	286
MS.gene23304.t1	MTR_4g070640	73.832	214	52	4	1	212	1	212	1.30e-87	257
MS.gene23304.t1	MTR_4g070660	69.643	224	56	5	1	212	1	224	6.68e-85	251
MS.gene23304.t1	MTR_3g117050	36.614	254	105	10	9	213	10	256	1.04e-28	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23304.t1	AT5G66440	30.864	243	131	8	1	211	1	238	1.62e-17	78.6

Find 27 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTCAAATCCTCTGTTCT+TGG	0.245245	4.3:+34529923	None:intergenic
AACTCAAGCGAGTATTTAAA+AGG	0.262024	4.3:-34530380	MS.gene23304:CDS
CGTTGAAGTTTGTGTCGTTT+TGG	0.324169	4.3:-34530447	MS.gene23304:CDS
GATGATAGTAAAGTTGAAAA+AGG	0.340575	4.3:-34530182	MS.gene23304:CDS
TAGCATTGCCTTTCTCAGTT+TGG	0.357534	4.3:+34530516	None:intergenic
ACGTTGTTCGCTCAAGGTTC+TGG	0.373057	4.3:-34530299	MS.gene23304:CDS
TGATGATATTTATGAAAAGT+TGG	0.403250	4.3:-34530249	MS.gene23304:CDS
CTCGTTGCTCATCTTATCCT+CGG	0.416320	4.3:+34529977	None:intergenic
TTTAATCTCCAAACTGAGAA+AGG	0.417953	4.3:-34530524	MS.gene23304:CDS
CTTTGAGGAATGGAGTCGTT+CGG	0.425044	4.3:-34530548	None:intergenic
AAGAAGAGTTATAGAAAGTG+TGG	0.434702	4.3:-34530122	MS.gene23304:CDS
CTTGAGTTTCTTACTGCAAC+CGG	0.445540	4.3:+34530395	None:intergenic
TAAAACATTTGAAGCTTCAA+AGG	0.454977	4.3:-34530490	MS.gene23304:CDS
TCCTCCTCAGCAACATGAGC+AGG	0.482114	4.3:+34530023	None:intergenic
AAAACATTTGAAGCTTCAAA+GGG	0.497511	4.3:-34530489	MS.gene23304:CDS
AACAATATCGTGTGTTGCGA+AGG	0.499204	4.3:-34530082	MS.gene23304:CDS
AATGCTTTCTATGAAACTAC+CGG	0.524823	4.3:-34530414	MS.gene23304:CDS
ATCATCACGTTGTTCGCTCA+AGG	0.531080	4.3:-34530305	MS.gene23304:CDS
TCGTTGCTCATCTTATCCTC+GGG	0.557561	4.3:+34529978	None:intergenic
ACCTGCTCATGTTGCTGAGG+AGG	0.563502	4.3:-34530024	MS.gene23304:CDS
AGAAGAGTTATAGAAAGTGT+GGG	0.575807	4.3:-34530121	MS.gene23304:CDS
GGAGTTTCGTCATATTGTAG+AGG	0.585827	4.3:-34529955	MS.gene23304:CDS
TGAACCTGCTCATGTTGCTG+AGG	0.604362	4.3:-34530027	MS.gene23304:CDS
GTGAGAGTGAGAACAATGGA+AGG	0.605246	4.3:-34530058	MS.gene23304:CDS
CTGAGGAGGAAATGATCGTG+AGG	0.652149	4.3:-34530010	MS.gene23304:CDS
AGGTGTGAGAGTGAGAACAA+TGG	0.658068	4.3:-34530062	MS.gene23304:CDS
CGAGGATAAGATGAGCAACG+AGG	0.729895	4.3:-34529976	MS.gene23304:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATTATTTTGTTTTTCATAT+CGG	-	chr4.3:34530121-34530140	MS.gene23304:CDS	15.0%
!!	CGATATGAAAAACAAAATAA+TGG	+	chr4.3:34530123-34530142	None:intergenic	20.0%
!!	TGATGATATTTATGAAAAGT+TGG	-	chr4.3:34530210-34530229	MS.gene23304:CDS	20.0%
!	AGGAACAAAAAATGAAAAGA+GGG	-	chr4.3:34530311-34530330	MS.gene23304:CDS	25.0%
!	GATGATAGTAAAGTTGAAAA+AGG	-	chr4.3:34530277-34530296	MS.gene23304:CDS	25.0%
!!	AAAACATTTGAAGCTTCAAA+GGG	-	chr4.3:34529970-34529989	MS.gene23304:CDS	25.0%
!!	TAAAACATTTGAAGCTTCAA+AGG	-	chr4.3:34529969-34529988	MS.gene23304:CDS	25.0%
!!	TTGAAGCTTCAAATGTTTTA+GGG	+	chr4.3:34529969-34529988	None:intergenic	25.0%
!!!	TTTGAAGCTTCAAATGTTTT+AGG	+	chr4.3:34529970-34529989	None:intergenic	25.0%
	AACTCAAGCGAGTATTTAAA+AGG	-	chr4.3:34530079-34530098	MS.gene23304:CDS	30.0%
	AAGAAGAGTTATAGAAAGTG+TGG	-	chr4.3:34530337-34530356	MS.gene23304:CDS	30.0%
	AATGCTTTCTATGAAACTAC+CGG	-	chr4.3:34530045-34530064	MS.gene23304:CDS	30.0%
	GAGGAACAAAAAATGAAAAG+AGG	-	chr4.3:34530310-34530329	MS.gene23304:CDS	30.0%
	GGAACAAAAAATGAAAAGAG+GGG	-	chr4.3:34530312-34530331	MS.gene23304:CDS	30.0%
	TTTAATCTCCAAACTGAGAA+AGG	-	chr4.3:34529935-34529954	MS.gene23304:CDS	30.0%
!	AGAAGAGTTATAGAAAGTGT+GGG	-	chr4.3:34530338-34530357	MS.gene23304:CDS	30.0%
	AACAATATCGTGTGTTGCGA+AGG	-	chr4.3:34530377-34530396	MS.gene23304:CDS	40.0%
	CACTTCAAATCCTCTGTTCT+TGG	+	chr4.3:34530539-34530558	None:intergenic	40.0%
	CTTGAGTTTCTTACTGCAAC+CGG	+	chr4.3:34530067-34530086	None:intergenic	40.0%
	GGAGTTTCGTCATATTGTAG+AGG	-	chr4.3:34530504-34530523	MS.gene23304:CDS	40.0%
	TAGCATTGCCTTTCTCAGTT+TGG	+	chr4.3:34529946-34529965	None:intergenic	40.0%
!	GCTTTTATTGCCAAGAACAG+AGG	-	chr4.3:34530526-34530545	MS.gene23304:CDS	40.0%
!	TGAAAAAGGTGATAGCGTAG+AGG	-	chr4.3:34530291-34530310	MS.gene23304:CDS	40.0%
!!!	CGTTGAAGTTTGTGTCGTTT+TGG	-	chr4.3:34530012-34530031	MS.gene23304:CDS	40.0%
	AGGTGTGAGAGTGAGAACAA+TGG	-	chr4.3:34530397-34530416	MS.gene23304:CDS	45.0%
	ATCATCACGTTGTTCGCTCA+AGG	-	chr4.3:34530154-34530173	MS.gene23304:CDS	45.0%
	CTCGTTGCTCATCTTATCCT+CGG	+	chr4.3:34530485-34530504	None:intergenic	45.0%
	GTGAGAGTGAGAACAATGGA+AGG	-	chr4.3:34530401-34530420	MS.gene23304:CDS	45.0%
	TCGTTGCTCATCTTATCCTC+GGG	+	chr4.3:34530484-34530503	None:intergenic	45.0%
	ACGTTGTTCGCTCAAGGTTC+TGG	-	chr4.3:34530160-34530179	MS.gene23304:CDS	50.0%
	CGAGGATAAGATGAGCAACG+AGG	-	chr4.3:34530483-34530502	MS.gene23304:CDS	50.0%
	CTGAGGAGGAAATGATCGTG+AGG	-	chr4.3:34530449-34530468	MS.gene23304:CDS	50.0%
	TGAACCTGCTCATGTTGCTG+AGG	-	chr4.3:34530432-34530451	MS.gene23304:CDS	50.0%
!	CGTGAGGATTTTGTATCCCG+AGG	-	chr4.3:34530465-34530484	MS.gene23304:CDS	50.0%
	ACCTGCTCATGTTGCTGAGG+AGG	-	chr4.3:34530435-34530454	MS.gene23304:CDS	55.0%
	TCCTCCTCAGCAACATGAGC+AGG	+	chr4.3:34530439-34530458	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	34529920	34530561	34529920	ID=MS.gene23304
chr4.3	mRNA	34529920	34530561	34529920	ID=MS.gene23304.t1;Parent=MS.gene23304
chr4.3	exon	34529920	34530561	34529920	ID=MS.gene23304.t1.exon1;Parent=MS.gene23304.t1
chr4.3	CDS	34529920	34530561	34529920	ID=cds.MS.gene23304.t1;Parent=MS.gene23304.t1

Gene Sequence

>MS.gene23304

ATGGAGTCGTTCGGTTTTAATCTCCAAACTGAGAAAGGCAATGCTACCCTAAAACATTTGAAGCTTCAAAGGGTAACAATCTTGTTACGACTCGTTGAAGTTTGTGTCGTTTTGGTGTTGATTTCAATGCTTTCTATGAAACTACCGGTTGCAGTAAGAAACTCAAGCGAGTATTTAAAAGGCTTTTCAGTCTTTAATAGCCATTATTTTGTTTTTCATATCGGAAACATTATAATCATCACGTTGTTCGCTCAAGGTTCTGGAAGAAATGTTGCAAATGAACAAGAATATGATGATATTTATGAAAAGTTGGTACGAAAAAGTATAAAGCACGAAGAAAAAGAGAAAATAAGAAAAGATGATAGTAAAGTTGAAAAAGGTGATAGCGTAGAGGAACAAAAAATGAAAAGAGGGGTGAAGAAGAGTTATAGAAAGTGTGGGAGTGAGAATTTAAAGAAACAATATCGTGTGTTGCGAAGGTGTGAGAGTGAGAACAATGGAAGGAAAAGCATTGAACCTGCTCATGTTGCTGAGGAGGAAATGATCGTGAGGATTTTGTATCCCGAGGATAAGATGAGCAACGAGGAGTTTCGTCATATTGTAGAGGCTTTTATTGCCAAGAACAGAGGATTTGAAGTGTAG

Protein sequence

>MS.gene23304.t1

MESFGFNLQTEKGNATLKHLKLQRVTILLRLVEVCVVLVLISMLSMKLPVAVRNSSEYLKGFSVFNSHYFVFHIGNIIIITLFAQGSGRNVANEQEYDDIYEKLVRKSIKHEEKEKIRKDDSKVEKGDSVEEQKMKRGVKKSYRKCGSENLKKQYRVLRRCESENNGRKSIEPAHVAEEEMIVRILYPEDKMSNEEFRHIVEAFIAKNRGFEV