AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28491


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28491.t1 MTR_8g078100 98.133 375 7 0 1 375 1 375 0.0 756
MS.gene28491.t1 MTR_5g019430 70.292 377 107 2 1 372 1 377 0.0 542
MS.gene28491.t1 MTR_4g069160 58.355 377 151 4 1 375 1 373 3.39e-147 421
MS.gene28491.t1 MTR_1g034480 55.733 375 159 3 1 374 1 369 5.35e-137 395
MS.gene28491.t1 MTR_3g011140 62.162 74 28 0 285 358 1 74 9.19e-26 99.8
MS.gene28491.t1 MTR_8g068520 22.293 314 206 7 76 373 86 377 6.06e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28491.t1 AT2G18630 54.354 379 167 2 1 374 2 379 2.15e-138 399
MS.gene28491.t1 AT2G18630 54.354 379 167 2 1 374 2 379 3.20e-138 399
MS.gene28491.t1 AT4G34320 55.319 376 159 5 1 375 1 368 6.37e-132 382
MS.gene28491.t1 AT4G34320 55.319 376 159 5 1 375 1 368 6.37e-132 382
MS.gene28491.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene28491.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene28491.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene28491.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene28491.t1 AT4G34330 48.851 348 162 3 24 371 17 348 1.48e-106 317
MS.gene28491.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene28491.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene28491.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene28491.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene28491.t1 AT5G66660 41.713 362 203 2 21 374 35 396 5.42e-85 263
MS.gene28491.t1 AT3G28270 27.933 358 242 6 28 370 16 372 6.11e-36 135
MS.gene28491.t1 AT3G28270 27.933 358 242 6 28 370 16 372 6.11e-36 135
MS.gene28491.t1 AT3G28290 25.661 378 242 12 24 370 11 380 4.66e-29 116
MS.gene28491.t1 AT3G28300 25.661 378 242 12 24 370 11 380 4.66e-29 116
MS.gene28491.t1 AT3G57100 25.705 319 210 5 65 370 43 347 2.05e-25 105
MS.gene28491.t1 AT3G28320 23.492 315 214 8 80 370 2 313 2.78e-20 90.9

Find 57 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATAAAACTCCTCCGTAAA+AGG 0.159235 8.4:+28506704 None:intergenic
TGAAGTTGTTAAAGTTATTT+TGG 0.163128 8.4:-28506972 MS.gene28491:CDS
TCATCAAAACTTTGCAAGTT+TGG 0.189922 8.4:+28507112 None:intergenic
TCGGGTGCTTACAAACAAAT+TGG 0.255573 8.4:-28506330 MS.gene28491:CDS
TGGATTGCAAGAGGGATATA+TGG 0.257789 8.4:-28506952 MS.gene28491:CDS
TTTGAAGAAGAGGTGCAAAA+TGG 0.287245 8.4:-28506791 MS.gene28491:CDS
TTACATTTCATTGAAGGATT+TGG 0.290195 8.4:-28506360 MS.gene28491:CDS
TTGCTACTGGTGTTGAAGTT+AGG 0.293911 8.4:-28507051 MS.gene28491:CDS
GGGATATGAGAAGGCTATAA+AGG 0.305251 8.4:-28506420 MS.gene28491:CDS
CATTCGGGTCGGTTGGAAAA+TGG 0.337016 8.4:-28506460 MS.gene28491:CDS
GCGGCGTTGGCTGTTCCATT+CGG 0.349853 8.4:-28506476 MS.gene28491:CDS
TGAAGGATTTGGACAACATT+CGG 0.353812 8.4:-28506349 MS.gene28491:CDS
AACAGCTTTGGCTGCGGCGT+TGG 0.383556 8.4:-28506489 MS.gene28491:CDS
GTTATTAGTTCTCTTGCTAC+TGG 0.384834 8.4:-28507064 MS.gene28491:CDS
CGGCGTTGGCTGTTCCATTC+GGG 0.389129 8.4:-28506475 MS.gene28491:CDS
GAAGGATTTGGACAACATTC+GGG 0.389768 8.4:-28506348 MS.gene28491:CDS
GGATTGCAAGAGGGATATAT+GGG 0.412828 8.4:-28506951 MS.gene28491:CDS
TCCGCCCGTTGTAACAGCTT+TGG 0.415627 8.4:-28506501 MS.gene28491:CDS
GAGGTGCAAAATGGGCTTGA+AGG 0.435928 8.4:-28506782 MS.gene28491:CDS
TTGAAGAAGAGGTGCAAAAT+GGG 0.439323 8.4:-28506790 MS.gene28491:CDS
AAGAGAACTAATAACCCTAT+TGG 0.447555 8.4:+28507072 None:intergenic
GGATATGAGAAGGCTATAAA+GGG 0.447749 8.4:-28506419 MS.gene28491:CDS
GTATTAAAGAGCATACCAAT+AGG 0.457807 8.4:-28507087 MS.gene28491:CDS
TGGTACTTACATTTCATTGA+AGG 0.469068 8.4:-28506366 MS.gene28491:CDS
GCAGCCAAAGCTGTTACAAC+GGG 0.472631 8.4:+28506497 None:intergenic
AATTACAAAACTCCAACGTC+TGG 0.474086 8.4:+28506879 None:intergenic
TCTAAGACCTCATCTCAATC+AGG 0.476204 8.4:+28506107 None:intergenic
AGGTGCAAAATGGGCTTGAA+GGG 0.480313 8.4:-28506781 MS.gene28491:CDS
CTCTTGAGAAATGTTTGAGA+CGG 0.491708 8.4:-28506856 MS.gene28491:CDS
GCTGTTCCATTCGGGTCGGT+TGG 0.492398 8.4:-28506467 MS.gene28491:CDS
TGAAGACATTGCAAGAGCTT+AGG 0.500716 8.4:-28506748 MS.gene28491:CDS
TCAATCGGTTTATACGCAGC+AGG 0.509528 8.4:-28506675 MS.gene28491:CDS
TATTAAAGAGCATACCAATA+GGG 0.511363 8.4:-28507086 MS.gene28491:CDS
CGTTGTAACAGCTTTGGCTG+CGG 0.511718 8.4:-28506495 MS.gene28491:CDS
AATAAAACTCCTCCGTAAAA+GGG 0.512251 8.4:+28506705 None:intergenic
TCTTGAGAAATGTTTGAGAC+GGG 0.517157 8.4:-28506855 MS.gene28491:CDS
CGCAGCCAAAGCTGTTACAA+CGG 0.524710 8.4:+28506496 None:intergenic
AAACAAGGATTTGTTCGCGT+TGG 0.546843 8.4:-28506927 MS.gene28491:CDS
AGGGAATTCAAGCAAGATGA+TGG 0.547310 8.4:-28507221 MS.gene28491:CDS
AGTTGAAATCACTCAAGTCA+TGG 0.549628 8.4:-28506604 MS.gene28491:CDS
GAAGGCTATAAAGGGACAAA+GGG 0.553000 8.4:-28506411 MS.gene28491:CDS
AGAAGGCTATAAAGGGACAA+AGG 0.553211 8.4:-28506412 MS.gene28491:CDS
GAATCAAGTGATTGTTAAGT+CGG 0.557993 8.4:-28506825 MS.gene28491:CDS
GCCAAAGCTGTTACAACGGG+CGG 0.569880 8.4:+28506500 None:intergenic
ATCTGAGGTATTAAGATGGG+AGG 0.576566 8.4:-28507241 None:intergenic
GTTGGCTGTTCCATTCGGGT+CGG 0.587471 8.4:-28506471 MS.gene28491:CDS
AATCACTCAAGTCATGGAAG+AGG 0.591019 8.4:-28506598 MS.gene28491:CDS
GATTTGCAATTTCTTCAACA+TGG 0.599579 8.4:+28506649 None:intergenic
TGATAATAGTCTCCAGACGT+TGG 0.605979 8.4:-28506891 MS.gene28491:CDS
GAGGGATATATGGGGAAACA+AGG 0.609168 8.4:-28506942 MS.gene28491:CDS
ATCACTCAAGTCATGGAAGA+GGG 0.610181 8.4:-28506597 MS.gene28491:CDS
GGTGCAAAATGGGCTTGAAG+GGG 0.611042 8.4:-28506780 MS.gene28491:CDS
GTAGTTAGCTCAATGCAGCT+TGG 0.613218 8.4:-28506386 MS.gene28491:CDS
TCTGAGGTATTAAGATGGGA+GGG 0.638076 8.4:-28507240 None:intergenic
GTGCAAAATGGGCTTGAAGG+GGG 0.641754 8.4:-28506779 MS.gene28491:CDS
GATTGCAAGAGGGATATATG+GGG 0.687000 8.4:-28506950 MS.gene28491:CDS
GTCGACAGATAAGAAAAGCG+AGG 0.701787 8.4:-28506160 MS.gene28491:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATATAGAAACTTTTTC+AGG - chr8.4:28507126-28507145 MS.gene28491:CDS 20.0%
!!! GTTTTATTTGTTGTTTCAAT+CGG - chr8.4:28506657-28506676 MS.gene28491:CDS 20.0%
!!! TGAAGTTGTTAAAGTTATTT+TGG - chr8.4:28506375-28506394 MS.gene28491:CDS 20.0%
! TTACATTTCATTGAAGGATT+TGG - chr8.4:28506987-28507006 MS.gene28491:CDS 25.0%
!! TATTAAAGAGCATACCAATA+GGG - chr8.4:28506261-28506280 MS.gene28491:CDS 25.0%
!! TGATTTTGCTCTTAAAAATG+AGG - chr8.4:28507071-28507090 MS.gene28491:CDS 25.0%
!!! GATTACTTATTTTGAAGAAG+AGG - chr8.4:28506546-28506565 MS.gene28491:CDS 25.0%
AAATAAAACTCCTCCGTAAA+AGG + chr8.4:28506646-28506665 None:intergenic 30.0%
AAGAGAACTAATAACCCTAT+TGG + chr8.4:28506278-28506297 None:intergenic 30.0%
AATAAAACTCCTCCGTAAAA+GGG + chr8.4:28506645-28506664 None:intergenic 30.0%
GAATCAAGTGATTGTTAAGT+CGG - chr8.4:28506522-28506541 MS.gene28491:CDS 30.0%
GATTTGCAATTTCTTCAACA+TGG + chr8.4:28506701-28506720 None:intergenic 30.0%
GCTAATGATTGCACAAAAAT+GGG - chr8.4:28506154-28506173 MS.gene28491:CDS 30.0%
TCATCAAAACTTTGCAAGTT+TGG + chr8.4:28506238-28506257 None:intergenic 30.0%
TGCTAATGATTGCACAAAAA+TGG - chr8.4:28506153-28506172 MS.gene28491:CDS 30.0%
TGGTACTTACATTTCATTGA+AGG - chr8.4:28506981-28507000 MS.gene28491:CDS 30.0%
! GTATTAAAGAGCATACCAAT+AGG - chr8.4:28506260-28506279 MS.gene28491:CDS 30.0%
!! ACTTTTTAAGGGATATGAGA+AGG - chr8.4:28506918-28506937 MS.gene28491:CDS 30.0%
!! TTAGGAATTTTAAGGATGCT+GGG - chr8.4:28506617-28506636 MS.gene28491:CDS 30.0%
!!! AAGTTATTTTGGATTGCAAG+AGG - chr8.4:28506386-28506405 MS.gene28491:CDS 30.0%
!!! AGAAACTTTTTCAGGAACAT+TGG - chr8.4:28507134-28507153 MS.gene28491:CDS 30.0%
!!! AGTTATTTTGGATTGCAAGA+GGG - chr8.4:28506387-28506406 MS.gene28491:CDS 30.0%
!!! GGTGTCAAATGCAATTTTTT+TGG - chr8.4:28506771-28506790 MS.gene28491:CDS 30.0%
AATTACAAAACTCCAACGTC+TGG + chr8.4:28506471-28506490 None:intergenic 35.0%
AGTTGAAATCACTCAAGTCA+TGG - chr8.4:28506743-28506762 MS.gene28491:CDS 35.0%
CTCTTGAGAAATGTTTGAGA+CGG - chr8.4:28506491-28506510 MS.gene28491:CDS 35.0%
GGATATGAGAAGGCTATAAA+GGG - chr8.4:28506928-28506947 MS.gene28491:CDS 35.0%
TGAAGGATTTGGACAACATT+CGG - chr8.4:28506998-28507017 MS.gene28491:CDS 35.0%
! GCAAGAGCTTAGGAATTTTA+AGG - chr8.4:28506609-28506628 MS.gene28491:CDS 35.0%
! GTTATTAGTTCTCTTGCTAC+TGG - chr8.4:28506283-28506302 MS.gene28491:CDS 35.0%
! TCTTGAGAAATGTTTGAGAC+GGG - chr8.4:28506492-28506511 MS.gene28491:CDS 35.0%
! TTGAAGAAGAGGTGCAAAAT+GGG - chr8.4:28506557-28506576 MS.gene28491:CDS 35.0%
! TTTGAAGAAGAGGTGCAAAA+TGG - chr8.4:28506556-28506575 MS.gene28491:CDS 35.0%
!! CTTAGGAATTTTAAGGATGC+TGG - chr8.4:28506616-28506635 MS.gene28491:CDS 35.0%
!! TAGGAATTTTAAGGATGCTG+GGG - chr8.4:28506618-28506637 MS.gene28491:CDS 35.0%
!!! ATGGTGCAACTCACTTTTTA+AGG - chr8.4:28506906-28506925 MS.gene28491:CDS 35.0%
!!! TGGTGCAACTCACTTTTTAA+GGG - chr8.4:28506907-28506926 MS.gene28491:CDS 35.0%
!!! TTTTTTGGCTGCGTTTGTTT+CGG - chr8.4:28506786-28506805 MS.gene28491:CDS 35.0%
AATCACTCAAGTCATGGAAG+AGG - chr8.4:28506749-28506768 MS.gene28491:CDS 40.0%
AGAAGGCTATAAAGGGACAA+AGG - chr8.4:28506935-28506954 MS.gene28491:CDS 40.0%
AGGGAATTCAAGCAAGATGA+TGG - chr8.4:28506126-28506145 MS.gene28491:CDS 40.0%
ATCACTCAAGTCATGGAAGA+GGG - chr8.4:28506750-28506769 MS.gene28491:CDS 40.0%
GAAGGATTTGGACAACATTC+GGG - chr8.4:28506999-28507018 MS.gene28491:CDS 40.0%
GAAGGCTATAAAGGGACAAA+GGG - chr8.4:28506936-28506955 MS.gene28491:CDS 40.0%
GATTGCAAGAGGGATATATG+GGG - chr8.4:28506397-28506416 MS.gene28491:CDS 40.0%
GGATTGCAAGAGGGATATAT+GGG - chr8.4:28506396-28506415 MS.gene28491:CDS 40.0%
GGGATATGAGAAGGCTATAA+AGG - chr8.4:28506927-28506946 MS.gene28491:CDS 40.0%
TCGGGTGCTTACAAACAAAT+TGG - chr8.4:28507017-28507036 MS.gene28491:CDS 40.0%
TGAAGACATTGCAAGAGCTT+AGG - chr8.4:28506599-28506618 MS.gene28491:CDS 40.0%
TGATAATAGTCTCCAGACGT+TGG - chr8.4:28506456-28506475 MS.gene28491:CDS 40.0%
TGGATTGCAAGAGGGATATA+TGG - chr8.4:28506395-28506414 MS.gene28491:CDS 40.0%
! AAACAAGGATTTGTTCGCGT+TGG - chr8.4:28506420-28506439 MS.gene28491:CDS 40.0%
! TTGCTACTGGTGTTGAAGTT+AGG - chr8.4:28506296-28506315 MS.gene28491:CDS 40.0%
!!! TTCGGTGTTGATTTTCTCTG+TGG - chr8.4:28506804-28506823 MS.gene28491:CDS 40.0%
GAGGGATATATGGGGAAACA+AGG - chr8.4:28506405-28506424 MS.gene28491:CDS 45.0%
GTAGTTAGCTCAATGCAGCT+TGG - chr8.4:28506961-28506980 MS.gene28491:CDS 45.0%
GTCGACAGATAAGAAAAGCG+AGG - chr8.4:28507187-28507206 MS.gene28491:CDS 45.0%
! AGGTGCAAAATGGGCTTGAA+GGG - chr8.4:28506566-28506585 MS.gene28491:CDS 45.0%
! TCAATCGGTTTATACGCAGC+AGG - chr8.4:28506672-28506691 MS.gene28491:CDS 45.0%
CATTCGGGTCGGTTGGAAAA+TGG - chr8.4:28506887-28506906 MS.gene28491:CDS 50.0%
CGCAGCCAAAGCTGTTACAA+CGG + chr8.4:28506854-28506873 None:intergenic 50.0%
GCAGCCAAAGCTGTTACAAC+GGG + chr8.4:28506853-28506872 None:intergenic 50.0%
! CATTTTCCAACCGACCCGAA+TGG + chr8.4:28506889-28506908 None:intergenic 50.0%
! CGTTGTAACAGCTTTGGCTG+CGG - chr8.4:28506852-28506871 MS.gene28491:CDS 50.0%
! GAGGTGCAAAATGGGCTTGA+AGG - chr8.4:28506565-28506584 MS.gene28491:CDS 50.0%
! GGTGCAAAATGGGCTTGAAG+GGG - chr8.4:28506567-28506586 MS.gene28491:CDS 50.0%
! GTGCAAAATGGGCTTGAAGG+GGG - chr8.4:28506568-28506587 MS.gene28491:CDS 50.0%
GCCAAAGCTGTTACAACGGG+CGG + chr8.4:28506850-28506869 None:intergenic 55.0%
GTTGGCTGTTCCATTCGGGT+CGG - chr8.4:28506876-28506895 MS.gene28491:CDS 55.0%
TCCGCCCGTTGTAACAGCTT+TGG - chr8.4:28506846-28506865 MS.gene28491:CDS 55.0%
! GGATGCTGGGGACCCTTTTA+CGG - chr8.4:28506630-28506649 MS.gene28491:CDS 55.0%
GCTGTTCCATTCGGGTCGGT+TGG - chr8.4:28506880-28506899 MS.gene28491:CDS 60.0%
! AACAGCTTTGGCTGCGGCGT+TGG - chr8.4:28506858-28506877 MS.gene28491:CDS 60.0%
! TGCTGGGGACCCTTTTACGG+AGG - chr8.4:28506633-28506652 MS.gene28491:CDS 60.0%
!! CGGCGTTGGCTGTTCCATTC+GGG - chr8.4:28506872-28506891 MS.gene28491:CDS 60.0%
!! GCGGCGTTGGCTGTTCCATT+CGG - chr8.4:28506871-28506890 MS.gene28491:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 28506121 28507248 28506121 ID=MS.gene28491
chr8.4 mRNA 28506121 28507248 28506121 ID=MS.gene28491.t1;Parent=MS.gene28491
chr8.4 exon 28506121 28507248 28506121 ID=MS.gene28491.t1.exon1;Parent=MS.gene28491.t1
chr8.4 CDS 28506121 28507248 28506121 ID=cds.MS.gene28491.t1;Parent=MS.gene28491.t1
Gene Sequence

>MS.gene28491

ATGGGAGGGAATTCAAGCAAGATGATGGTTGATGCTAATGATTGCACAAAAATGGGTACTCATTCTGTGTATGCAGCTGATTTAAGCTCATATGAAGCTGCATGTATCAAAGATCCAAACTTGCAAAGTTTTGATGAGAGTATTAAAGAGCATACCAATAGGGTTATTAGTTCTCTTGCTACTGGTGTTGAAGTTAGGTCTTTATCTTTCGATTCGCTGCGAGAAGTTACTGATAGTTTGCTTGAGATGAATCATGAAGTTGTTAAAGTTATTTTGGATTGCAAGAGGGATATATGGGGAAACAAGGATTTGTTCGCGTTGGTTAATGATTATTTTGATAATAGTCTCCAGACGTTGGAGTTTTGTAATTCTCTTGAGAAATGTTTGAGACGGGCACGCGAGAATCAAGTGATTGTTAAGTCGGTGATTACTTATTTTGAAGAAGAGGTGCAAAATGGGCTTGAAGGGGGAACTTGTGTGAAGACATTGCAAGAGCTTAGGAATTTTAAGGATGCTGGGGACCCTTTTACGGAGGAGTTTTATTTGTTGTTTCAATCGGTTTATACGCAGCAGGCAACCATGTTGAAGAAATTGCAAATCAGAAAGAGAAAGCTTGATAAGAAGTTGAAATCACTCAAGTCATGGAAGAGGGTGTCAAATGCAATTTTTTTGGCTGCGTTTGTTTCGGTGTTGATTTTCTCTGTGGTTGCAGCTTCTATTGCTGCTCCGCCCGTTGTAACAGCTTTGGCTGCGGCGTTGGCTGTTCCATTCGGGTCGGTTGGAAAATGGTGCAACTCACTTTTTAAGGGATATGAGAAGGCTATAAAGGGACAAAGGGAAGTAGTTAGCTCAATGCAGCTTGGTACTTACATTTCATTGAAGGATTTGGACAACATTCGGGTGCTTACAAACAAATTGGAACTACAGCTTGAATCGTTATTGCAGAATGCTGATTTTGCTCTTAAAAATGAGGATGTGATAAAGCTTGCAATCGATGAGATTAAGAAGAATATAGAAACTTTTTCAGGAACATTGGAAACTCTAAGTGCAAATGCTGATAAATGCAGTCGACAGATAAGAAAAGCGAGGACAGTCGTTATACAAAATATAATCAAAAGACCTGATTGA

Protein sequence

>MS.gene28491.t1

MGGNSSKMMVDANDCTKMGTHSVYAADLSSYEAACIKDPNLQSFDESIKEHTNRVISSLATGVEVRSLSFDSLREVTDSLLEMNHEVVKVILDCKRDIWGNKDLFALVNDYFDNSLQTLEFCNSLEKCLRRARENQVIVKSVITYFEEEVQNGLEGGTCVKTLQELRNFKDAGDPFTEEFYLLFQSVYTQQATMLKKLQIRKRKLDKKLKSLKSWKRVSNAIFLAAFVSVLIFSVVAASIAAPPVVTALAAALAVPFGSVGKWCNSLFKGYEKAIKGQREVVSSMQLGTYISLKDLDNIRVLTNKLELQLESLLQNADFALKNEDVIKLAIDEIKKNIETFSGTLETLSANADKCSRQIRKARTVVIQNIIKRPD