AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28762

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28762.t1 MTR_1g115920 93.103 464 28 2 119 579 372 834 0.0 799
MS.gene28762.t1 MTR_1g115920 83.582 67 7 2 3 67 82 146 1.17e-21 100
MS.gene28762.t1 MTR_1g115920 68.421 76 19 2 66 136 241 316 1.36e-13 74.3
MS.gene28762.t1 MTR_4g094355 81.560 282 16 3 25 306 6 251 1.61e-141 417
MS.gene28762.t1 MTR_4g094355 92.147 191 15 0 389 579 250 440 2.04e-121 365
MS.gene28762.t1 MTR_5g039050 84.305 223 26 3 269 491 67 280 3.56e-110 336
MS.gene28762.t1 MTR_5g039050 82.222 45 7 1 131 174 6 50 1.65e-14 76.3
MS.gene28762.t1 MTR_5g039100 72.464 69 14 2 2 67 38 104 1.27e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28762.t1 AT1G28060 66.811 461 146 4 119 576 330 786 0.0 603
MS.gene28762.t1 AT1G28060 66.811 461 146 4 119 576 330 786 0.0 603
MS.gene28762.t1 AT1G28060 66.811 461 146 4 119 576 330 786 0.0 603
MS.gene28762.t1 AT3G55930 61.852 270 99 3 306 573 3 270 1.99e-97 298

Find 143 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACACCAGAACACATTTAT+TGG 0.127318 4.3:-19862482 None:intergenic
ATAAAATTGAAGAGTAAATT+TGG 0.190886 4.3:+19861602 MS.gene28762:CDS
TCTCCTTAGCCATACACTTT+TGG 0.199636 4.3:-19861970 None:intergenic
TGCTGGGGTTCCTCATTATT+GGG 0.233213 4.3:+19862591 MS.gene28762:CDS
AATTGGATGATAAGAGAATT+AGG 0.237950 4.3:+19861039 MS.gene28762:CDS
ATGCTGGGGTTCCTCATTAT+TGG 0.242299 4.3:+19862590 MS.gene28762:CDS
CTTCTGCATTCTGGAAATTA+TGG 0.253483 4.3:+19861785 MS.gene28762:CDS
ACACACTTGTCTCACTTTAC+AGG 0.257138 4.3:+19862242 MS.gene28762:CDS
ACAAGTGTGTCCAATGTATT+TGG 0.267435 4.3:-19862229 None:intergenic
CTCATCTTGACCTTTGGTTT+TGG 0.279974 4.3:-19862034 None:intergenic
CTTTGCTAGCTGTGCTTGTT+TGG 0.291419 4.3:-19861651 None:intergenic
TCCTGTTTAGTTGGCTTGAT+AGG 0.304342 4.3:-19861929 None:intergenic
ATTAAGTTCGGATTGATATC+AGG 0.315138 4.3:-19861686 None:intergenic
AAGCAACCTTAGCACATTAA+AGG 0.318390 4.3:+19861397 MS.gene28762:CDS
TTCTTCTGCTCCTGTTTAGT+TGG 0.318787 4.3:-19861938 None:intergenic
GGTGGAGGGGCGGGCTCAGC+AGG 0.333316 4.3:-19861899 None:intergenic
GACGTGGCACTTCTGCATTC+TGG 0.335270 4.3:+19861776 MS.gene28762:CDS
GTTCATCTATTTCACGTCCC+TGG 0.343803 4.3:-19861346 None:intergenic
ATGATAAGAGAATTAGGGTT+AGG 0.350518 4.3:+19861045 MS.gene28762:CDS
GAACCTATTGAAACTCGATT+TGG 0.351326 4.3:-19862518 None:intergenic
TGAAATACTAAAACCTGTAT+TGG 0.355353 4.3:+19861451 MS.gene28762:CDS
AGATGAAGGCAATATGAAAA+TGG 0.363556 4.3:+19861832 MS.gene28762:CDS
AAGAAGGAACGTAGGAGATT+TGG 0.374683 4.3:+19861080 MS.gene28762:CDS
AAATTACTCATCTTGACCTT+TGG 0.381357 4.3:-19862040 None:intergenic
TTGCCAAATCGAGTTTCAAT+AGG 0.388491 4.3:+19862515 MS.gene28762:CDS
ATTGGATGATAAGAGAATTA+GGG 0.389557 4.3:+19861040 MS.gene28762:CDS
AGGTTGAGGTGGTGGAGGGG+CGG 0.390911 4.3:-19861909 None:intergenic
TGTTTGTGGAGGAAGGAAAA+TGG 0.392737 4.3:+19861561 MS.gene28762:CDS
TCTGTAATTTCTATTAAGTT+CGG 0.393587 4.3:-19861698 None:intergenic
CTCGATACTCCTTGCATAAG+AGG 0.401486 4.3:+19860988 MS.gene28762:CDS
TTGATAGGTTGAGGTGGTGG+AGG 0.401991 4.3:-19861914 None:intergenic
AGCAAGTCAATTAAGAGGTA+TGG 0.404160 4.3:+19862385 MS.gene28762:CDS
TTCTTCTCACGCAGCTCAGC+AGG 0.405655 4.3:-19862184 None:intergenic
TCAAAATGAGGATTAGATTC+AGG 0.407433 4.3:-19861482 None:intergenic
TGCAGCTGCTGAGCGTGAGC+AGG 0.412035 4.3:+19862126 MS.gene28762:CDS
ACCTATCAAGCCAACTAAAC+AGG 0.413749 4.3:+19861928 MS.gene28762:CDS
TTCATCTATTTCACGTCCCT+GGG 0.414957 4.3:-19861345 None:intergenic
GGTTGAGGTGGTGGAGGGGC+GGG 0.419674 4.3:-19861908 None:intergenic
ATATAGCGAGGATCGGGAAT+TGG 0.420235 4.3:+19861022 MS.gene28762:CDS
TTATGCTTAGACGCATCAAT+TGG 0.421874 4.3:+19862413 MS.gene28762:CDS
GAATCTACAGCTATGTTTCC+TGG 0.422589 4.3:+19861254 MS.gene28762:CDS
TTTAATGTGCTAAGGTTGCT+TGG 0.430099 4.3:-19861395 None:intergenic
CACCACTCAATATCCGGAAT+CGG 0.431295 4.3:-19861752 None:intergenic
GGCTCAGCAGGCGGTTCAAT+AGG 0.433119 4.3:-19861887 None:intergenic
TAACGTCGGGTGTAGCAATC+TGG 0.434175 4.3:-19861277 None:intergenic
ATAAACTGTAGAGCGGGCCT+TGG 0.434948 4.3:-19862284 None:intergenic
CAAAGCTCCTGCGGCCTAAG+AGG 0.436122 4.3:+19861528 MS.gene28762:CDS
AACTTTACGAGCAGCTGCTT+CGG 0.436123 4.3:-19862560 None:intergenic
GGCTTGATAGGTTGAGGTGG+TGG 0.440923 4.3:-19861917 None:intergenic
TGTTCTTCGTCTTGACGCCC+AGG 0.441585 4.3:+19861328 MS.gene28762:CDS
CGATGCCAATAAATGTGTTC+TGG 0.441704 4.3:+19862477 MS.gene28762:CDS
AACAGAAAATTCATCCTCTT+AGG 0.449563 4.3:-19861542 None:intergenic
GGAGGGGCGGGCTCAGCAGG+CGG 0.453459 4.3:-19861896 None:intergenic
AAAACTGAAGGACCCGATTC+CGG 0.455391 4.3:+19861739 MS.gene28762:CDS
TGATAAGAGAATTAGGGTTA+GGG 0.455771 4.3:+19861046 MS.gene28762:CDS
AGTAATTTACATAAGGTACT+AGG 0.456676 4.3:+19862055 MS.gene28762:CDS
ACGTCCCACCACTCAATATC+CGG 0.458176 4.3:-19861758 None:intergenic
ATTAGTGTTGTCGTGGTTGA+AGG 0.473394 4.3:+19862358 MS.gene28762:CDS
TGCTGAGCTGCGTGAGAAGA+AGG 0.474449 4.3:+19862186 MS.gene28762:CDS
AGATCCCACCCGGCTTGAAA+AGG 0.474598 4.3:+19862093 MS.gene28762:CDS
AGGAGATTTGGTGATAAACT+TGG 0.481118 4.3:+19861092 MS.gene28762:CDS
CACACTTGTCTCACTTTACA+GGG 0.483911 4.3:+19862243 MS.gene28762:CDS
TAGAGAATATAGCGAGGATC+GGG 0.485543 4.3:+19861016 MS.gene28762:CDS
GTGAGCAGGCTCACATAGAT+AGG 0.485650 4.3:+19862140 MS.gene28762:CDS
ACTAAGGACCCAAAAGTGTA+TGG 0.489044 4.3:+19861961 MS.gene28762:CDS
GCTCAACAAAATCGTTTGAC+TGG 0.491510 4.3:+19862313 MS.gene28762:CDS
TAGGGTTTCTGAAGCAAAGA+AGG 0.491982 4.3:+19861064 MS.gene28762:CDS
TATTGACCTTTAATGTGCTA+AGG 0.494808 4.3:-19861403 None:intergenic
CAAGATGAGTAATTTACATA+AGG 0.497213 4.3:+19862048 MS.gene28762:CDS
AACAGGAGCAGAAGAAACTA+AGG 0.497775 4.3:+19861945 MS.gene28762:CDS
TGGGCGTCAAGACGAAGAAC+AGG 0.500740 4.3:-19861326 None:intergenic
TCAGGATCGACATCCAATAC+AGG 0.501479 4.3:-19861464 None:intergenic
GCCTTCATCTATAGTACCGT+TGG 0.504964 4.3:-19861819 None:intergenic
GGAGCCTTGGTTGGTTTCTG+AGG 0.506975 4.3:-19861305 None:intergenic
TGCCTTAGCAAAGGCTAAGA+AGG 0.507867 4.3:+19861133 MS.gene28762:CDS
TGATAGGTTGAGGTGGTGGA+GGG 0.509364 4.3:-19861913 None:intergenic
ACCACTCAATATCCGGAATC+GGG 0.511345 4.3:-19861751 None:intergenic
GCAAGTCAATTAAGAGGTAT+GGG 0.512247 4.3:+19862386 MS.gene28762:CDS
CAAGTGTGTCCAATGTATTT+GGG 0.514012 4.3:-19862228 None:intergenic
TAAACTGTAGAGCGGGCCTT+GGG 0.515526 4.3:-19862283 None:intergenic
CGAAGAACAGGAGCCTTGGT+TGG 0.517067 4.3:-19861314 None:intergenic
ACTTTCATGTGGAAATTCCT+CGG 0.518196 4.3:+19861864 MS.gene28762:CDS
GTGGAAGCAAGTCAATTAAG+AGG 0.522404 4.3:+19862380 MS.gene28762:CDS
GGCTAAGGAGAAAGCACGAC+AGG 0.533388 4.3:+19861982 MS.gene28762:CDS
GAGCCTTGGTTGGTTTCTGA+GGG 0.533893 4.3:-19861304 None:intergenic
AGCTCAAGCAAAAGAGCAGA+AGG 0.535132 4.3:+19861628 MS.gene28762:CDS
TCTATCTTTCCTCTTATGCA+AGG 0.536070 4.3:-19860997 None:intergenic
GGATATTGAGTGGTGGGACG+TGG 0.536150 4.3:+19861760 MS.gene28762:CDS
GTTTCATGACCCAAATACAT+TGG 0.539983 4.3:+19862219 MS.gene28762:CDS
AGCTGCGTGAGAAGAAGGAA+AGG 0.547194 4.3:+19862191 MS.gene28762:CDS
ATCGAAGTGCGATGAAGAGA+TGG 0.548205 4.3:+19860959 MS.gene28762:CDS
CCCATTCTCTCATCAAAATG+AGG 0.549338 4.3:-19861494 None:intergenic
GACCCAAAAGTGTATGGCTA+AGG 0.549762 4.3:+19861967 MS.gene28762:CDS
GACGAAAGAGAGAGTAGCAA+AGG 0.549906 4.3:+19860927 MS.gene28762:CDS
ATAGAGAATATAGCGAGGAT+CGG 0.549922 4.3:+19861015 MS.gene28762:CDS
CAAACAAGCACAGCTAGCAA+AGG 0.555686 4.3:+19861652 MS.gene28762:CDS
TTCTGAGGGACAGTAACGTC+GGG 0.556866 4.3:-19861290 None:intergenic
AAGACGAAGAACAGGAGCCT+TGG 0.557281 4.3:-19861318 None:intergenic
TTCATCTATAGTACCGTTGG+CGG 0.558630 4.3:-19861816 None:intergenic
GTTCTTCGTCTTGACGCCCA+GGG 0.562372 4.3:+19861329 MS.gene28762:CDS
TCGGGTGTAGCAATCTGGCC+AGG 0.563408 4.3:-19861272 None:intergenic
AATTCATCCTCTTAGGCCGC+AGG 0.563858 4.3:-19861535 None:intergenic
GGAAGAATTCAACTTTCATG+TGG 0.569338 4.3:+19861853 MS.gene28762:CDS
AGTGTTGTCGTGGTTGAAGG+TGG 0.570940 4.3:+19862361 MS.gene28762:CDS
TTAGTTGGCTTGATAGGTTG+AGG 0.576255 4.3:-19861923 None:intergenic
ATTAACATAAACTGTAGAGC+GGG 0.580679 4.3:-19862290 None:intergenic
GTAATTTACATAAGGTACTA+GGG 0.581923 4.3:+19862056 MS.gene28762:CDS
AGCCTTCTTAGCCTTTGCTA+AGG 0.581971 4.3:-19861135 None:intergenic
GATAGGTTGAGGTGGTGGAG+GGG 0.582077 4.3:-19861912 None:intergenic
GTTGGCTTGATAGGTTGAGG+TGG 0.586185 4.3:-19861920 None:intergenic
GCCAACGGTACTATAGATGA+AGG 0.586217 4.3:+19861818 MS.gene28762:CDS
CTAAGGCATCACTACTAGAG+AGG 0.588190 4.3:-19861118 None:intergenic
TATGGTGACATTGCCGCCAA+CGG 0.595136 4.3:+19861803 MS.gene28762:CDS
TGATGGTATTAGTGTTGTCG+TGG 0.603393 4.3:+19862351 MS.gene28762:CDS
GACAGGGATATGGGATACGA+TGG 0.607091 4.3:+19860903 None:intergenic
CATTTGTATAGTTGACAGCA+TGG 0.608274 4.3:-19862615 None:intergenic
AAAGAGAGAGTAGCAAAGGT+AGG 0.609445 4.3:+19860931 MS.gene28762:CDS
CGACAGGAGATGATTAGAGA+GGG 0.610590 4.3:+19861998 MS.gene28762:CDS
CGATTCCGGATATTGAGTGG+TGG 0.614030 4.3:+19861753 MS.gene28762:CDS
AAATGACTTCTCTCATCCCA+AGG 0.614270 4.3:+19862267 MS.gene28762:CDS
GGATGTGCGGTGATTTGTGA+TGG 0.616908 4.3:+19862334 MS.gene28762:CDS
TGTAATGAAAGCAAAACTGA+AGG 0.617069 4.3:+19861727 MS.gene28762:CDS
ACAGCATGGTCCCAATAATG+AGG 0.617442 4.3:-19862601 None:intergenic
GAATTTGAGTTATTCAACTG+AGG 0.620565 4.3:-19861200 None:intergenic
TAGTAGTGATGCCTTAGCAA+AGG 0.620911 4.3:+19861124 MS.gene28762:CDS
AGCACAGCTAGCAAAGGCAA+AGG 0.626359 4.3:+19861658 MS.gene28762:CDS
GCAGGCGGTTCAATAGGCCG+AGG 0.628346 4.3:-19861881 None:intergenic
CATAGAACCGCCAAAACCAA+AGG 0.629032 4.3:+19862024 MS.gene28762:CDS
CCAATAAATGTGTTCTGGTG+TGG 0.635559 4.3:+19862482 MS.gene28762:CDS
ACGACAGGAGATGATTAGAG+AGG 0.638900 4.3:+19861997 MS.gene28762:CDS
TTTCTGAGGGACAGTAACGT+CGG 0.642738 4.3:-19861291 None:intergenic
GATTCCGGATATTGAGTGGT+GGG 0.645660 4.3:+19861754 MS.gene28762:CDS
ACCCGATTCCGGATATTGAG+TGG 0.647460 4.3:+19861750 MS.gene28762:CDS
GGACGTGAAATAGATGAACA+TGG 0.655823 4.3:+19861350 MS.gene28762:CDS
AAATGTGTTCTGGTGTGGCA+AGG 0.657323 4.3:+19862487 MS.gene28762:CDS
GAAGTGCGATGAAGAGATGG+AGG 0.664040 4.3:+19860962 MS.gene28762:CDS
GAAAGATAGAGAATATAGCG+AGG 0.672126 4.3:+19861010 MS.gene28762:CDS
CTGAAGCAAAGAAGGAACGT+AGG 0.673233 4.3:+19861072 MS.gene28762:CDS
AAATCGTTTGACTGGATGTG+CGG 0.693768 4.3:+19862321 MS.gene28762:CDS
TCGACAAAACAAAGCTCCTG+CGG 0.696622 4.3:+19861519 MS.gene28762:CDS
AAGCAACTCAAGATCCCACC+CGG 0.703568 4.3:+19862083 MS.gene28762:CDS
CATTAACATAAACTGTAGAG+CGG 0.704797 4.3:-19862291 None:intergenic
TGTCCCTCAGAAACCAACCA+AGG 0.741969 4.3:+19861301 MS.gene28762:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTGAAGAGTAAATT+TGG + chr4.3:19861602-19861621 MS.gene28762:CDS 15.0%
!! TCTGTAATTTCTATTAAGTT+CGG - chr4.3:19861701-19861720 None:intergenic 20.0%
! AATTGGATGATAAGAGAATT+AGG + chr4.3:19861039-19861058 MS.gene28762:CDS 25.0%
! ATTGGATGATAAGAGAATTA+GGG + chr4.3:19861040-19861059 MS.gene28762:CDS 25.0%
! CAAGATGAGTAATTTACATA+AGG + chr4.3:19862048-19862067 MS.gene28762:CDS 25.0%
! TGAAATACTAAAACCTGTAT+TGG + chr4.3:19861451-19861470 MS.gene28762:CDS 25.0%
!! AGTAATTTACATAAGGTACT+AGG + chr4.3:19862055-19862074 MS.gene28762:CDS 25.0%
!! GTAATTTACATAAGGTACTA+GGG + chr4.3:19862056-19862075 MS.gene28762:CDS 25.0%
AAATTACTCATCTTGACCTT+TGG - chr4.3:19862043-19862062 None:intergenic 30.0%
AACAGAAAATTCATCCTCTT+AGG - chr4.3:19861545-19861564 None:intergenic 30.0%
AGATGAAGGCAATATGAAAA+TGG + chr4.3:19861832-19861851 MS.gene28762:CDS 30.0%
ATGATAAGAGAATTAGGGTT+AGG + chr4.3:19861045-19861064 MS.gene28762:CDS 30.0%
ATTAACATAAACTGTAGAGC+GGG - chr4.3:19862293-19862312 None:intergenic 30.0%
ATTAAGTTCGGATTGATATC+AGG - chr4.3:19861689-19861708 None:intergenic 30.0%
CATTAACATAAACTGTAGAG+CGG - chr4.3:19862294-19862313 None:intergenic 30.0%
TATTGACCTTTAATGTGCTA+AGG - chr4.3:19861406-19861425 None:intergenic 30.0%
TCAAAATGAGGATTAGATTC+AGG - chr4.3:19861485-19861504 None:intergenic 30.0%
TGATAAGAGAATTAGGGTTA+GGG + chr4.3:19861046-19861065 MS.gene28762:CDS 30.0%
TGTAATGAAAGCAAAACTGA+AGG + chr4.3:19861727-19861746 MS.gene28762:CDS 30.0%
! GAATTTGAGTTATTCAACTG+AGG - chr4.3:19861203-19861222 None:intergenic 30.0%
AAGCAACCTTAGCACATTAA+AGG + chr4.3:19861397-19861416 MS.gene28762:CDS 35.0%
ACAAGTGTGTCCAATGTATT+TGG - chr4.3:19862232-19862251 None:intergenic 35.0%
ACTTTCATGTGGAAATTCCT+CGG + chr4.3:19861864-19861883 MS.gene28762:CDS 35.0%
AGCAAGTCAATTAAGAGGTA+TGG + chr4.3:19862385-19862404 MS.gene28762:CDS 35.0%
ATAGAGAATATAGCGAGGAT+CGG + chr4.3:19861015-19861034 MS.gene28762:CDS 35.0%
CAAGTGTGTCCAATGTATTT+GGG - chr4.3:19862231-19862250 None:intergenic 35.0%
CATTTGTATAGTTGACAGCA+TGG - chr4.3:19862618-19862637 None:intergenic 35.0%
GAAAGATAGAGAATATAGCG+AGG + chr4.3:19861010-19861029 MS.gene28762:CDS 35.0%
GAACCTATTGAAACTCGATT+TGG - chr4.3:19862521-19862540 None:intergenic 35.0%
GGAAGAATTCAACTTTCATG+TGG + chr4.3:19861853-19861872 MS.gene28762:CDS 35.0%
GTTTCATGACCCAAATACAT+TGG + chr4.3:19862219-19862238 MS.gene28762:CDS 35.0%
TCTATCTTTCCTCTTATGCA+AGG - chr4.3:19861000-19861019 None:intergenic 35.0%
TTATGCTTAGACGCATCAAT+TGG + chr4.3:19862413-19862432 MS.gene28762:CDS 35.0%
TTGCCAAATCGAGTTTCAAT+AGG + chr4.3:19862515-19862534 MS.gene28762:CDS 35.0%
TTTAATGTGCTAAGGTTGCT+TGG - chr4.3:19861398-19861417 None:intergenic 35.0%
! AATTTTCTGTTTGTGGAGGA+AGG + chr4.3:19861554-19861573 MS.gene28762:CDS 35.0%
! CTTCTGCATTCTGGAAATTA+TGG + chr4.3:19861785-19861804 MS.gene28762:CDS 35.0%
! GAGGATGAATTTTCTGTTTG+TGG + chr4.3:19861547-19861566 MS.gene28762:CDS 35.0%
! GATGAATTTTCTGTTTGTGG+AGG + chr4.3:19861550-19861569 MS.gene28762:CDS 35.0%
! GCAAGTCAATTAAGAGGTAT+GGG + chr4.3:19862386-19862405 MS.gene28762:CDS 35.0%
! GTAAAGTTTTTGTGGATGCT+GGG + chr4.3:19862575-19862594 MS.gene28762:CDS 35.0%
! TAAAGTTTTTGTGGATGCTG+GGG + chr4.3:19862576-19862595 MS.gene28762:CDS 35.0%
!! AGGAGATTTGGTGATAAACT+TGG + chr4.3:19861092-19861111 MS.gene28762:CDS 35.0%
!! CCTCATTTTGATGAGAGAAT+GGG + chr4.3:19861494-19861513 MS.gene28762:CDS 35.0%
!! TCCTCATTTTGATGAGAGAA+TGG + chr4.3:19861493-19861512 MS.gene28762:CDS 35.0%
AAAGAGAGAGTAGCAAAGGT+AGG + chr4.3:19860931-19860950 MS.gene28762:CDS 40.0%
AAATCGTTTGACTGGATGTG+CGG + chr4.3:19862321-19862340 MS.gene28762:CDS 40.0%
AAATGACTTCTCTCATCCCA+AGG + chr4.3:19862267-19862286 MS.gene28762:CDS 40.0%
AACAGGAGCAGAAGAAACTA+AGG + chr4.3:19861945-19861964 MS.gene28762:CDS 40.0%
AAGAAGGAACGTAGGAGATT+TGG + chr4.3:19861080-19861099 MS.gene28762:CDS 40.0%
ACACACTTGTCTCACTTTAC+AGG + chr4.3:19862242-19862261 MS.gene28762:CDS 40.0%
ACCTATCAAGCCAACTAAAC+AGG + chr4.3:19861928-19861947 MS.gene28762:CDS 40.0%
ATTAGTGTTGTCGTGGTTGA+AGG + chr4.3:19862358-19862377 MS.gene28762:CDS 40.0%
CACACTTGTCTCACTTTACA+GGG + chr4.3:19862243-19862262 MS.gene28762:CDS 40.0%
CCAATAAATGTGTTCTGGTG+TGG + chr4.3:19862482-19862501 MS.gene28762:CDS 40.0%
CCACACCAGAACACATTTAT+TGG - chr4.3:19862485-19862504 None:intergenic 40.0%
CCCATTCTCTCATCAAAATG+AGG - chr4.3:19861497-19861516 None:intergenic 40.0%
CGATGCCAATAAATGTGTTC+TGG + chr4.3:19862477-19862496 MS.gene28762:CDS 40.0%
GAATCTACAGCTATGTTTCC+TGG + chr4.3:19861254-19861273 MS.gene28762:CDS 40.0%
GCTCAACAAAATCGTTTGAC+TGG + chr4.3:19862313-19862332 MS.gene28762:CDS 40.0%
GGACGTGAAATAGATGAACA+TGG + chr4.3:19861350-19861369 MS.gene28762:CDS 40.0%
GTGGAAGCAAGTCAATTAAG+AGG + chr4.3:19862380-19862399 MS.gene28762:CDS 40.0%
TAGAGAATATAGCGAGGATC+GGG + chr4.3:19861016-19861035 MS.gene28762:CDS 40.0%
TAGTAGTGATGCCTTAGCAA+AGG + chr4.3:19861124-19861143 MS.gene28762:CDS 40.0%
TGATGGTATTAGTGTTGTCG+TGG + chr4.3:19862351-19862370 MS.gene28762:CDS 40.0%
TGTTTGTGGAGGAAGGAAAA+TGG + chr4.3:19861561-19861580 MS.gene28762:CDS 40.0%
TTCATCTATAGTACCGTTGG+CGG - chr4.3:19861819-19861838 None:intergenic 40.0%
TTCATCTATTTCACGTCCCT+GGG - chr4.3:19861348-19861367 None:intergenic 40.0%
TTCTTCTGCTCCTGTTTAGT+TGG - chr4.3:19861941-19861960 None:intergenic 40.0%
! ACTAAGGACCCAAAAGTGTA+TGG + chr4.3:19861961-19861980 MS.gene28762:CDS 40.0%
! AGCTGCTCGTAAAGTTTTTG+TGG + chr4.3:19862567-19862586 MS.gene28762:CDS 40.0%
! CGTAAAGTTTTTGTGGATGC+TGG + chr4.3:19862574-19862593 MS.gene28762:CDS 40.0%
! CTCCTTAGCCATACACTTTT+GGG - chr4.3:19861972-19861991 None:intergenic 40.0%
! TCTCCTTAGCCATACACTTT+TGG - chr4.3:19861973-19861992 None:intergenic 40.0%
!! CTCATCTTGACCTTTGGTTT+TGG - chr4.3:19862037-19862056 None:intergenic 40.0%
!! TACGTACTTCCTTTTCAAGC+CGG - chr4.3:19862105-19862124 None:intergenic 40.0%
!! TAGGGTTTCTGAAGCAAAGA+AGG + chr4.3:19861064-19861083 MS.gene28762:CDS 40.0%
!! TCCTGTTTAGTTGGCTTGAT+AGG - chr4.3:19861932-19861951 None:intergenic 40.0%
!! TTAGTTGGCTTGATAGGTTG+AGG - chr4.3:19861926-19861945 None:intergenic 40.0%
!!! ATCTTGACCTTTGGTTTTGG+CGG - chr4.3:19862034-19862053 None:intergenic 40.0%
AAAACTGAAGGACCCGATTC+CGG + chr4.3:19861739-19861758 MS.gene28762:CDS 45.0%
AAATGTGTTCTGGTGTGGCA+AGG + chr4.3:19862487-19862506 MS.gene28762:CDS 45.0%
AACTTTACGAGCAGCTGCTT+CGG - chr4.3:19862563-19862582 None:intergenic 45.0%
ACAGCATGGTCCCAATAATG+AGG - chr4.3:19862604-19862623 None:intergenic 45.0%
ACCACTCAATATCCGGAATC+GGG - chr4.3:19861754-19861773 None:intergenic 45.0%
ACGACAGGAGATGATTAGAG+AGG + chr4.3:19861997-19862016 MS.gene28762:CDS 45.0%
AGCTCAAGCAAAAGAGCAGA+AGG + chr4.3:19861628-19861647 MS.gene28762:CDS 45.0%
ATATAGCGAGGATCGGGAAT+TGG + chr4.3:19861022-19861041 MS.gene28762:CDS 45.0%
ATCGAAGTGCGATGAAGAGA+TGG + chr4.3:19860959-19860978 MS.gene28762:CDS 45.0%
ATGCTGGGGTTCCTCATTAT+TGG + chr4.3:19862590-19862609 MS.gene28762:CDS 45.0%
CAAACAAGCACAGCTAGCAA+AGG + chr4.3:19861652-19861671 MS.gene28762:CDS 45.0%
CACCACTCAATATCCGGAAT+CGG - chr4.3:19861755-19861774 None:intergenic 45.0%
CATAGAACCGCCAAAACCAA+AGG + chr4.3:19862024-19862043 MS.gene28762:CDS 45.0%
CGACAGGAGATGATTAGAGA+GGG + chr4.3:19861998-19862017 MS.gene28762:CDS 45.0%
CTCGATACTCCTTGCATAAG+AGG + chr4.3:19860988-19861007 MS.gene28762:CDS 45.0%
CTGAAGCAAAGAAGGAACGT+AGG + chr4.3:19861072-19861091 MS.gene28762:CDS 45.0%
CTTTGCTAGCTGTGCTTGTT+TGG - chr4.3:19861654-19861673 None:intergenic 45.0%
GACGAAAGAGAGAGTAGCAA+AGG + chr4.3:19860927-19860946 MS.gene28762:CDS 45.0%
GATTCCGGATATTGAGTGGT+GGG + chr4.3:19861754-19861773 MS.gene28762:CDS 45.0%
GCCAACGGTACTATAGATGA+AGG + chr4.3:19861818-19861837 MS.gene28762:CDS 45.0%
GCCTTCATCTATAGTACCGT+TGG - chr4.3:19861822-19861841 None:intergenic 45.0%
GTTCATCTATTTCACGTCCC+TGG - chr4.3:19861349-19861368 None:intergenic 45.0%
TCAGGATCGACATCCAATAC+AGG - chr4.3:19861467-19861486 None:intergenic 45.0%
TCGACAAAACAAAGCTCCTG+CGG + chr4.3:19861519-19861538 MS.gene28762:CDS 45.0%
TGCCTTAGCAAAGGCTAAGA+AGG + chr4.3:19861133-19861152 MS.gene28762:CDS 45.0%
TGCTGGGGTTCCTCATTATT+GGG + chr4.3:19862591-19862610 MS.gene28762:CDS 45.0%
! AGCCTTCTTAGCCTTTGCTA+AGG - chr4.3:19861138-19861157 None:intergenic 45.0%
! CTAAGGCATCACTACTAGAG+AGG - chr4.3:19861121-19861140 None:intergenic 45.0%
! GACCCAAAAGTGTATGGCTA+AGG + chr4.3:19861967-19861986 MS.gene28762:CDS 45.0%
! TTTCTGAGGGACAGTAACGT+CGG - chr4.3:19861294-19861313 None:intergenic 45.0%
!! ACGTACTTCCTTTTCAAGCC+GGG - chr4.3:19862104-19862123 None:intergenic 45.0%
AAGACGAAGAACAGGAGCCT+TGG - chr4.3:19861321-19861340 None:intergenic 50.0%
AAGCAACTCAAGATCCCACC+CGG + chr4.3:19862083-19862102 MS.gene28762:CDS 50.0%
AATTCATCCTCTTAGGCCGC+AGG - chr4.3:19861538-19861557 None:intergenic 50.0%
ACCCGATTCCGGATATTGAG+TGG + chr4.3:19861750-19861769 MS.gene28762:CDS 50.0%
ACGTCCCACCACTCAATATC+CGG - chr4.3:19861761-19861780 None:intergenic 50.0%
AGCACAGCTAGCAAAGGCAA+AGG + chr4.3:19861658-19861677 MS.gene28762:CDS 50.0%
AGCTGCGTGAGAAGAAGGAA+AGG + chr4.3:19862191-19862210 MS.gene28762:CDS 50.0%
AGTGTTGTCGTGGTTGAAGG+TGG + chr4.3:19862361-19862380 MS.gene28762:CDS 50.0%
ATAAACTGTAGAGCGGGCCT+TGG - chr4.3:19862287-19862306 None:intergenic 50.0%
CGATTCCGGATATTGAGTGG+TGG + chr4.3:19861753-19861772 MS.gene28762:CDS 50.0%
GAAGTGCGATGAAGAGATGG+AGG + chr4.3:19860962-19860981 MS.gene28762:CDS 50.0%
GTGAGCAGGCTCACATAGAT+AGG + chr4.3:19862140-19862159 MS.gene28762:CDS 50.0%
TAAACTGTAGAGCGGGCCTT+GGG - chr4.3:19862286-19862305 None:intergenic 50.0%
TAACGTCGGGTGTAGCAATC+TGG - chr4.3:19861280-19861299 None:intergenic 50.0%
TATGGTGACATTGCCGCCAA+CGG + chr4.3:19861803-19861822 MS.gene28762:CDS 50.0%
TGTCCCTCAGAAACCAACCA+AGG + chr4.3:19861301-19861320 MS.gene28762:CDS 50.0%
! GGATGTGCGGTGATTTGTGA+TGG + chr4.3:19862334-19862353 MS.gene28762:CDS 50.0%
! TTCTGAGGGACAGTAACGTC+GGG - chr4.3:19861293-19861312 None:intergenic 50.0%
! TTGATAGGTTGAGGTGGTGG+AGG - chr4.3:19861917-19861936 None:intergenic 50.0%
!! ACTTCCTTTTCAAGCCGGGT+GGG - chr4.3:19862100-19862119 None:intergenic 50.0%
!! GAGCCTTGGTTGGTTTCTGA+GGG - chr4.3:19861307-19861326 None:intergenic 50.0%
!! GTTGGCTTGATAGGTTGAGG+TGG - chr4.3:19861923-19861942 None:intergenic 50.0%
!! TACTTCCTTTTCAAGCCGGG+TGG - chr4.3:19862101-19862120 None:intergenic 50.0%
!! TGATAGGTTGAGGTGGTGGA+GGG - chr4.3:19861916-19861935 None:intergenic 50.0%
CGAAGAACAGGAGCCTTGGT+TGG - chr4.3:19861317-19861336 None:intergenic 55.0%
GACGTGGCACTTCTGCATTC+TGG + chr4.3:19861776-19861795 MS.gene28762:CDS 55.0%
GTTCTTCGTCTTGACGCCCA+GGG + chr4.3:19861329-19861348 MS.gene28762:CDS 55.0%
TGCTGAGCTGCGTGAGAAGA+AGG + chr4.3:19862186-19862205 MS.gene28762:CDS 55.0%
TGGGCGTCAAGACGAAGAAC+AGG - chr4.3:19861329-19861348 None:intergenic 55.0%
TGTTCTTCGTCTTGACGCCC+AGG + chr4.3:19861328-19861347 MS.gene28762:CDS 55.0%
TTCTTCTCACGCAGCTCAGC+AGG - chr4.3:19862187-19862206 None:intergenic 55.0%
! AGATCCCACCCGGCTTGAAA+AGG + chr4.3:19862093-19862112 MS.gene28762:CDS 55.0%
! GGATATTGAGTGGTGGGACG+TGG + chr4.3:19861760-19861779 MS.gene28762:CDS 55.0%
! GGCTAAGGAGAAAGCACGAC+AGG + chr4.3:19861982-19862001 MS.gene28762:CDS 55.0%
! GGCTTGATAGGTTGAGGTGG+TGG - chr4.3:19861920-19861939 None:intergenic 55.0%
!! GATAGGTTGAGGTGGTGGAG+GGG - chr4.3:19861915-19861934 None:intergenic 55.0%
!! GGAGCCTTGGTTGGTTTCTG+AGG - chr4.3:19861308-19861327 None:intergenic 55.0%
CAAAGCTCCTGCGGCCTAAG+AGG + chr4.3:19861528-19861547 MS.gene28762:CDS 60.0%
GGCTCAGCAGGCGGTTCAAT+AGG - chr4.3:19861890-19861909 None:intergenic 60.0%
TCGGGTGTAGCAATCTGGCC+AGG - chr4.3:19861275-19861294 None:intergenic 60.0%
GCAGGCGGTTCAATAGGCCG+AGG - chr4.3:19861884-19861903 None:intergenic 65.0%
TGCAGCTGCTGAGCGTGAGC+AGG + chr4.3:19862126-19862145 MS.gene28762:CDS 65.0%
!! AGGTTGAGGTGGTGGAGGGG+CGG - chr4.3:19861912-19861931 None:intergenic 65.0%
!! GGTTGAGGTGGTGGAGGGGC+GGG - chr4.3:19861911-19861930 None:intergenic 70.0%
!! GGAGGGGCGGGCTCAGCAGG+CGG - chr4.3:19861899-19861918 None:intergenic 80.0%
!! GGTGGAGGGGCGGGCTCAGC+AGG - chr4.3:19861902-19861921 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 19860912 19862651 19860912 ID=MS.gene28762
chr4.3 mRNA 19860912 19862651 19860912 ID=MS.gene28762.t1;Parent=MS.gene28762
chr4.3 exon 19860912 19862651 19860912 ID=MS.gene28762.t1.exon1;Parent=MS.gene28762.t1
chr4.3 CDS 19860912 19862651 19860912 ID=cds.MS.gene28762.t1;Parent=MS.gene28762.t1
Gene Sequence

>MS.gene28762

ATGGGATACGATGGAGACGAAAGAGAGAGTAGCAAAGGTAGGTCTAAATCGAAGTGCGATGAAGAGATGGAGGATTCTCGATACTCCTTGCATAAGAGGAAAGATAGAGAATATAGCGAGGATCGGGAATTGGATGATAAGAGAATTAGGGTTAGGGTTTCTGAAGCAAAGAAGGAACGTAGGAGATTTGGTGATAAACTTGGAAGCCTCTCTAGTAGTGATGCCTTAGCAAAGGCTAAGAAGGCTTTACAAATGCAGAAATTGTTGTCAGAGAAGCTGAAGAAAATTCCTCAGTTGAATAACTCAAATTCAGAAGAAAGCACACAGTTGTGTAACAAGACTGAATCTACAGCTATGTTTCCTGGCCAGATTGCTACACCCGACGTTACTGTCCCTCAGAAACCAACCAAGGCTCCTGTTCTTCGTCTTGACGCCCAGGGACGTGAAATAGATGAACATGGAAATGTTGTTAATGTAACTAAGCCAAGCAACCTTAGCACATTAAAGGTCAATATTAATAAACACAAGAGAGATGCATTTGAAATACTAAAACCTGTATTGGATGTCGATCCTGAATCTAATCCTCATTTTGATGAGAGAATGGGTATCGACAAAACAAAGCTCCTGCGGCCTAAGAGGATGAATTTTCTGTTTGTGGAGGAAGGAAAATGGTCAAGAGACGCTGAATCAATAAAATTGAAGAGTAAATTTGGAGAAGCTCAAGCAAAAGAGCAGAAGGCCAAACAAGCACAGCTAGCAAAGGCAAAGGCTTCTCCTGATATCAATCCGAACTTAATAGAAATTACAGAGAGAGTTGTAATGAAAGCAAAACTGAAGGACCCGATTCCGGATATTGAGTGGTGGGACGTGGCACTTCTGCATTCTGGAAATTATGGTGACATTGCCGCCAACGGTACTATAGATGAAGGCAATATGAAAATGGAAGAATTCAACTTTCATGTGGAAATTCCTCGGCCTATTGAACCGCCTGCTGAGCCCGCCCCTCCACCACCTCAACCTATCAAGCCAACTAAACAGGAGCAGAAGAAACTAAGGACCCAAAAGTGTATGGCTAAGGAGAAAGCACGACAGGAGATGATTAGAGAGGGTGTCATAGAACCGCCAAAACCAAAGGTCAAGATGAGTAATTTACATAAGGTACTAGGGACTGAAGCAACTCAAGATCCCACCCGGCTTGAAAAGGAAGTACGTAATGCAGCTGCTGAGCGTGAGCAGGCTCACATAGATAGGAATATTGCACGCAAGCTTACTCCTGCTGAGCTGCGTGAGAAGAAGGAAAGGAAGTTGTTTCATGACCCAAATACATTGGACACACTTGTCTCACTTTACAGGGTAAATGACTTCTCTCATCCCAAGGCCCGCTCTACAGTTTATGTTAATGCTCAACAAAATCGTTTGACTGGATGTGCGGTGATTTGTGATGGTATTAGTGTTGTCGTGGTTGAAGGTGGAAGCAAGTCAATTAAGAGGTATGGGAAACTTATGCTTAGACGCATCAATTGGAATGATGTTTCAAAAGAGAAAGAAGATAATGAAGATTCAGACGATGCCAATAAATGTGTTCTGGTGTGGCAAGGAAGTGTTGCCAAATCGAGTTTCAATAGGTTCAGTGTTCATGACTGCATCACCGAAGCAGCTGCTCGTAAAGTTTTTGTGGATGCTGGGGTTCCTCATTATTGGGACCATGCTGTCAACTATACAAATGATGATGCTGTTTGA
Protein sequence

>MS.gene28762.t1

MGYDGDERESSKGRSKSKCDEEMEDSRYSLHKRKDREYSEDRELDDKRIRVRVSEAKKERRRFGDKLGSLSSSDALAKAKKALQMQKLLSEKLKKIPQLNNSNSEESTQLCNKTESTAMFPGQIATPDVTVPQKPTKAPVLRLDAQGREIDEHGNVVNVTKPSNLSTLKVNINKHKRDAFEILKPVLDVDPESNPHFDERMGIDKTKLLRPKRMNFLFVEEGKWSRDAESIKLKSKFGEAQAKEQKAKQAQLAKAKASPDINPNLIEITERVVMKAKLKDPIPDIEWWDVALLHSGNYGDIAANGTIDEGNMKMEEFNFHVEIPRPIEPPAEPAPPPPQPIKPTKQEQKKLRTQKCMAKEKARQEMIREGVIEPPKPKVKMSNLHKVLGTEATQDPTRLEKEVRNAAAEREQAHIDRNIARKLTPAELREKKERKLFHDPNTLDTLVSLYRVNDFSHPKARSTVYVNAQQNRLTGCAVICDGISVVVVEGGSKSIKRYGKLMLRRINWNDVSKEKEDNEDSDDANKCVLVWQGSVAKSSFNRFSVHDCITEAAARKVFVDAGVPHYWDHAVNYTNDDAV