AlfalfaGEDB | MS.gene007200

M. sativa cultivar XinJiangDaYe / MS.gene007200

KEGG: K11099
GO: GO:0000387 GO:0005575 GO:0005681 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0022618 GO:0032991 GO:0034622 GO:0043933 GO:0065003 GO:0071826 GO:0071840 GO:1990904
PFAM: PF01423.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007200.t1	XP_003625846.1	100	80	0	0	1	80	1	80	2.50E-35	157.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007200.t1	O82221	91.1	79	7	0	1	79	1	79	1.4e-34	146.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007200.t1	Q2HTF4	100.0	80	0	0	1	80	1	80	1.8e-35	157.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene007200	MS.gene004548	PPI
MS.gene007200	MS.gene70292	PPI
MS.gene007200	MS.gene67483	PPI
MS.gene046119	MS.gene007200	PPI
MS.gene028322	MS.gene007200	PPI
MS.gene58148	MS.gene007200	PPI
MS.gene53517	MS.gene007200	PPI
MS.gene68906	MS.gene007200	PPI
MS.gene007200	MS.gene043867	PPI
MS.gene64804	MS.gene007200	PPI
MS.gene007200	MS.gene065836	PPI
MS.gene007200	MS.gene018076	PPI
MS.gene007200	MS.gene35136	PPI
MS.gene007200	MS.gene073888	PPI
MS.gene007200	MS.gene93333	PPI
MS.gene90813	MS.gene007200	PPI
MS.gene015624	MS.gene007200	PPI
MS.gene024669	MS.gene007200	PPI
MS.gene24692	MS.gene007200	PPI
MS.gene28172	MS.gene007200	PPI
MS.gene75072	MS.gene007200	PPI
MS.gene073734	MS.gene007200	PPI
MS.gene030285	MS.gene007200	PPI
MS.gene007200	MS.gene28762	PPI
MS.gene013607	MS.gene007200	PPI
MS.gene82776	MS.gene007200	PPI
MS.gene48652	MS.gene007200	PPI
MS.gene058567	MS.gene007200	PPI
MS.gene065857	MS.gene007200	PPI
MS.gene007200	MS.gene067444	PPI
MS.gene36469	MS.gene007200	PPI
MS.gene065862	MS.gene007200	PPI
MS.gene56983	MS.gene007200	PPI
MS.gene007200	MS.gene070731	PPI
MS.gene053099	MS.gene007200	PPI
MS.gene52398	MS.gene007200	PPI
MS.gene007200	MS.gene85931	PPI
MS.gene065853	MS.gene007200	PPI
MS.gene06729	MS.gene007200	PPI
MS.gene007200	MS.gene86968	PPI
MS.gene032681	MS.gene007200	PPI
MS.gene028393	MS.gene007200	PPI
MS.gene007200	MS.gene22321	PPI
MS.gene072740	MS.gene007200	PPI
MS.gene007200	MS.gene00289	PPI
MS.gene050303	MS.gene007200	PPI
MS.gene030697	MS.gene007200	PPI
MS.gene59503	MS.gene007200	PPI
MS.gene007200	MS.gene062632	PPI
MS.gene007200	MS.gene66329	PPI
MS.gene007200	MS.gene047872	PPI
MS.gene009798	MS.gene007200	PPI
MS.gene66321	MS.gene007200	PPI
MS.gene04711	MS.gene007200	PPI
MS.gene50211	MS.gene007200	PPI
MS.gene007200	MS.gene060774	PPI
MS.gene83322	MS.gene007200	PPI
MS.gene007200	MS.gene035486	PPI
MS.gene007200	MS.gene023131	PPI
MS.gene92231	MS.gene007200	PPI
MS.gene069779	MS.gene007200	PPI
MS.gene011973	MS.gene007200	PPI
MS.gene21651	MS.gene007200	PPI
MS.gene007200	MS.gene97334	PPI
MS.gene95848	MS.gene007200	PPI
MS.gene040757	MS.gene007200	PPI
MS.gene064051	MS.gene007200	PPI
MS.gene007200	MS.gene053443	PPI
MS.gene62660	MS.gene007200	PPI
MS.gene06946	MS.gene007200	PPI
MS.gene029082	MS.gene007200	PPI
MS.gene83565	MS.gene007200	PPI
MS.gene007200	MS.gene35064	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007200.t1	MTR_7g104930	100.000	80	0	0	1	80	1	80	1.30e-53	161
MS.gene007200.t1	MTR_6g066250	90.476	63	6	0	14	76	15	77	5.20e-35	114
MS.gene007200.t1	MTR_7g065100	38.028	71	35	1	9	70	10	80	9.19e-13	58.5
MS.gene007200.t1	MTR_8g036205	34.722	72	38	1	9	71	10	81	5.11e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene007200.t1	AT2G23930	91.139	79	7	0	1	79	1	79	2.77e-49	150
MS.gene007200.t1	AT3G11500	91.139	79	7	0	1	79	1	79	4.56e-49	149
MS.gene007200.t1	AT3G11500	91.139	79	7	0	1	79	1	79	4.56e-49	149
MS.gene007200.t1	AT2G23930	88.679	53	6	0	27	79	1	53	1.63e-28	97.1
MS.gene007200.t1	AT2G03870	38.028	71	35	1	9	70	10	80	1.59e-12	57.8
MS.gene007200.t1	AT2G03870	38.028	71	35	1	9	70	10	80	1.59e-12	57.8

Find 18 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCGACCAATTTATGAATT+TGG	0.200064	7.4:-7712512	MS.gene007200:CDS
CAACAACCAAATTCATAAAT+TGG	0.293346	7.4:+7712506	None:intergenic
AATGCTTACTTCTTCAAATC+TGG	0.328903	7.4:+7713522	None:intergenic
TCTTTCACCATGAGTAGGTC+AGG	0.413563	7.4:-7713552	None:intergenic
AATTAGATGCTTCTGTTCAC+TGG	0.418853	7.4:+7712279	None:intergenic
AATGCAAATCGCATGATTGT+TGG	0.502115	7.4:-7712547	MS.gene007200:CDS
TCATAAATTGGTCGAAGCCA+CGG	0.529169	7.4:+7712518	None:intergenic
CGCTTTCTGCAGGTGATCAG+AGG	0.541314	7.4:-7712333	MS.gene007200:intron
TTTGTTCTTTCACCATGAGT+AGG	0.559149	7.4:-7713557	None:intergenic
GACAATACTGTGGAGGTGAA+TGG	0.565835	7.4:-7712484	MS.gene007200:CDS
TGGCTGCCCTGACCTACTCA+TGG	0.567702	7.4:+7713545	None:intergenic
AAATAGTGTGGTCACTGTAG+AGG	0.581425	7.4:-7712310	MS.gene007200:CDS
CTTTCACCATGAGTAGGTCA+GGG	0.590203	7.4:-7713551	None:intergenic
GCTTACTTCTTCAAATCTGG+TGG	0.598528	7.4:+7713525	None:intergenic
ATGATTGTTGGTACTCTCCG+TGG	0.628986	7.4:-7712535	MS.gene007200:CDS
TTTGGTTGTTGACAATACTG+TGG	0.664176	7.4:-7712494	MS.gene007200:CDS
GGTTGTTGACAATACTGTGG+AGG	0.683546	7.4:-7712491	MS.gene007200:CDS
GGTGATCAGAGGAAATAGTG+TGG	0.728293	7.4:-7712322	MS.gene007200:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CCATTAAAAAAAAAAAAAAA+AGG	-	chr7.4:7712575-7712594	MS.gene007200:intron	10.0%
!!!	CCTTTTTTTTTTTTTTTTAA+TGG	+	chr7.4:7712578-7712597	None:intergenic	10.0%
!!!	TAATTTTTTAACTGAATTTT+TGG	-	chr7.4:7712648-7712667	MS.gene007200:intron	10.0%
!!	TATTCATATAAATCTTTCTA+CGG	-	chr7.4:7713074-7713093	MS.gene007200:intron	15.0%
!!	ATATGGTAGCTTATATTATT+TGG	-	chr7.4:7712933-7712952	MS.gene007200:intron	20.0%
!!	ATTCATAAAGGAATGAAAAA+CGG	+	chr7.4:7713021-7713040	None:intergenic	20.0%
!!	ATTCATATAAATCTTTCTAC+GGG	-	chr7.4:7713075-7713094	MS.gene007200:intron	20.0%
!!!	TATGCATTTTTTTTTTCGAT+AGG	-	chr7.4:7712402-7712421	MS.gene007200:intron	20.0%
!!!	TTTTCATTCCTTTATGAATT+GGG	-	chr7.4:7713022-7713041	MS.gene007200:intron	20.0%
!!!	TTTTTCATTCCTTTATGAAT+TGG	-	chr7.4:7713021-7713040	MS.gene007200:intron	20.0%
!	ACATGTGTATAAATGGATAT+TGG	-	chr7.4:7713173-7713192	MS.gene007200:intron	25.0%
!	ATATGTATCTATGTACATGT+GGG	-	chr7.4:7713146-7713165	MS.gene007200:intron	25.0%
!	CAACAACCAAATTCATAAAT+TGG	+	chr7.4:7713321-7713340	None:intergenic	25.0%
!	TAACCACTTCTTCTATTAAT+TGG	+	chr7.4:7712791-7712810	None:intergenic	25.0%
!	TTTCATTCCTTTATGAATTG+GGG	-	chr7.4:7713023-7713042	MS.gene007200:intron	25.0%
!!!	TTTTTTTTTCGATAGGTACA+TGG	-	chr7.4:7712409-7712428	MS.gene007200:intron	25.0%
	AATGCTTACTTCTTCAAATC+TGG	+	chr7.4:7712305-7712324	None:intergenic	30.0%
	AGGAATTATACTGAAGTATG+TGG	-	chr7.4:7713233-7713252	MS.gene007200:intron	30.0%
	CATGTACATAGATACATATC+AGG	+	chr7.4:7713145-7713164	None:intergenic	30.0%
	CTTCGACCAATTTATGAATT+TGG	-	chr7.4:7713312-7713331	MS.gene007200:intron	30.0%
	GATATGTATCTATGTACATG+TGG	-	chr7.4:7713145-7713164	MS.gene007200:intron	30.0%
	GATCCAATTAATAGAAGAAG+TGG	-	chr7.4:7712785-7712804	MS.gene007200:intron	30.0%
	GCAATGAGAAAAATGACATA+GGG	-	chr7.4:7713362-7713381	MS.gene007200:intron	30.0%
	GGGTTTAACATGTGTATAAA+TGG	-	chr7.4:7713166-7713185	MS.gene007200:intron	30.0%
	TGTAATTTCTCTCGCTTATA+TGG	-	chr7.4:7712916-7712935	MS.gene007200:intron	30.0%
	TTCATTCCTTTATGAATTGG+GGG	-	chr7.4:7713024-7713043	MS.gene007200:intron	30.0%
!	TTTTGAATTTCTACTGCACT+TGG	-	chr7.4:7712369-7712388	MS.gene007200:intron	30.0%
!!	ACTTGATAATGCATAGTATG+AGG	+	chr7.4:7713400-7713419	None:intergenic	30.0%
!!	TTTTGTAGTTTGTGTGAAGA+TGG	-	chr7.4:7712600-7712619	MS.gene007200:intron	30.0%
	AATGCAAATCGCATGATTGT+TGG	-	chr7.4:7713277-7713296	MS.gene007200:intron	35.0%
	CATGTCTTATGATTTAGCTG+AGG	-	chr7.4:7713213-7713232	MS.gene007200:intron	35.0%
	GCATTATAAAGTGAAGTTCG+AGG	-	chr7.4:7712955-7712974	MS.gene007200:intron	35.0%
	GGCAATGAGAAAAATGACAT+AGG	-	chr7.4:7713361-7713380	MS.gene007200:intron	35.0%
	TGAGAAAAATGACATAGGGA+TGG	-	chr7.4:7713366-7713385	MS.gene007200:intron	35.0%
	TTCTAGGTACAAGATTGAGT+GGG	-	chr7.4:7712699-7712718	MS.gene007200:intron	35.0%
	TTTCTAGGTACAAGATTGAG+TGG	-	chr7.4:7712698-7712717	MS.gene007200:intron	35.0%
	TTTGGTTGTTGACAATACTG+TGG	-	chr7.4:7713330-7713349	MS.gene007200:intron	35.0%
	AAATAGTGTGGTCACTGTAG+AGG	-	chr7.4:7713514-7713533	MS.gene007200:intron	40.0%
	CACTATTGCTATGAGTTCAG+TGG	-	chr7.4:7712675-7712694	MS.gene007200:intron	40.0%
	CATATCAGGAACAAACAGAC+AGG	+	chr7.4:7713131-7713150	None:intergenic	40.0%
	CATCTTATAGAGCTTGAGCT+AGG	-	chr7.4:7713445-7713464	MS.gene007200:intron	40.0%
	CGTAATCCCCCAATTCATAA+AGG	+	chr7.4:7713033-7713052	None:intergenic	40.0%
	CTATGAGTTCAGTGGTTTCT+AGG	-	chr7.4:7712683-7712702	MS.gene007200:intron	40.0%
	GAAAAATGACATAGGGATGG+TGG	-	chr7.4:7713369-7713388	MS.gene007200:intron	40.0%
	GCTTACTTCTTCAAATCTGG+TGG	+	chr7.4:7712302-7712321	None:intergenic	40.0%
	GGACAAACAGCTTCAAAGTA+AGG	-	chr7.4:7712430-7712449	MS.gene007200:intron	40.0%
	TCATAAATTGGTCGAAGCCA+CGG	+	chr7.4:7713309-7713328	None:intergenic	40.0%
	TGGATTGCAGAGAAAAAGTG+TGG	-	chr7.4:7712863-7712882	MS.gene007200:intron	40.0%
!	AATTTTCACTCGCTTTCTGC+AGG	-	chr7.4:7713481-7713500	MS.gene007200:intron	40.0%
!	CTGTATGTGAGAGCTTTTAC+TGG	-	chr7.4:7712826-7712845	MS.gene007200:intron	40.0%
	ATGATTGTTGGTACTCTCCG+TGG	-	chr7.4:7713289-7713308	MS.gene007200:intron	45.0%
	GACAATACTGTGGAGGTGAA+TGG	-	chr7.4:7713340-7713359	MS.gene007200:intron	45.0%
	GGTGATCAGAGGAAATAGTG+TGG	-	chr7.4:7713502-7713521	MS.gene007200:intron	45.0%
	GGTTGTTGACAATACTGTGG+AGG	-	chr7.4:7713333-7713352	MS.gene007200:intron	45.0%
!	TACTGGCTGCGAACATTCTT+TGG	-	chr7.4:7712843-7712862	MS.gene007200:intron	45.0%
!!	CTATATATATAAATATAAAA+AGG	+	chr7.4:7712551-7712570	None:intergenic	5.0%
!!!	TTTTTTTTTTAAATCAAATT+TGG	-	chr7.4:7712502-7712521	MS.gene007200:CDS	5.0%
	GTGTGGCATGTCTTTGCTAC+TGG	-	chr7.4:7712880-7712899	MS.gene007200:intron	50.0%
	CGCTTTCTGCAGGTGATCAG+AGG	-	chr7.4:7713491-7713510	MS.gene007200:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	7712281	7713565	7712281	ID=MS.gene007200
chr7.4	mRNA	7712281	7713565	7712281	ID=MS.gene007200.t1;Parent=MS.gene007200
chr7.4	exon	7713531	7713565	7713531	ID=MS.gene007200.t1.exon1;Parent=MS.gene007200.t1
chr7.4	CDS	7713531	7713565	7713531	ID=cds.MS.gene007200.t1;Parent=MS.gene007200.t1
chr7.4	exon	7713400	7713422	7713400	ID=MS.gene007200.t1.exon2;Parent=MS.gene007200.t1
chr7.4	CDS	7713400	7713422	7713400	ID=cds.MS.gene007200.t1;Parent=MS.gene007200.t1
chr7.4	exon	7712456	7712577	7712456	ID=MS.gene007200.t1.exon3;Parent=MS.gene007200.t1
chr7.4	CDS	7712456	7712577	7712456	ID=cds.MS.gene007200.t1;Parent=MS.gene007200.t1
chr7.4	exon	7712281	7712343	7712281	ID=MS.gene007200.t1.exon4;Parent=MS.gene007200.t1
chr7.4	CDS	7712281	7712343	7712281	ID=cds.MS.gene007200.t1;Parent=MS.gene007200.t1

Gene Sequence

>MS.gene007200

ATGAGTAGGTCAGGGCAGCCACCAGATTTGAAGAAGTAAGCATTCTTCACACTTTTCAATTCATTTTTTCATTACAATTTTTCGTTGATTTTGAATTTCTACTGCACTTGGTCGATACTGATATGCATTTTTTTTTTCGATAGGTACATGGACAAACAGCTTCAAAGTAAGGTTCTTTCTGCTAATTGTTCTTACTTCTTATCTTCTTTCTTTCTTTGTTTTTTTTTTTTTAAATCAAATTTGGTTTTTTGTAGTATTTGTTCAGTTCCTTTTTATATTTATATATATAGTTTGCCATTAAAAAAAAAAAAAAAAGGTTTTTTGTAGTTTGTGTGAAGATGGAGTGTTTAGTAGAATTGCAAAAATATAATTTTTTAACTGAATTTTTGGTTTTCACTATTGCTATGAGTTCAGTGGTTTCTAGGTACAAGATTGAGTGGGATTTTGATTTTTTATCAATGAATAGCTGAAGATTGTTATGTTCAACTTATAATATTTAAGCATGATCCAATTAATAGAAGAAGTGGTTAAGAATGTATGAGTGTCTGTATGTGAGAGCTTTTACTGGCTGCGAACATTCTTTGGATTGCAGAGAAAAAGTGTGGCATGTCTTTGCTACTGGTTGATACTTTGAATGTAATTTCTCTCGCTTATATGGTAGCTTATATTATTTGGCATTATAAAGTGAAGTTCGAGGAAGTCAATTACTCAATTACAGCTTAATCATTATTTTTTATCCGTTTTTCATTCCTTTATGAATTGGGGGATTACGCTTATTAGTGATTGTGTGAACTATTCATATAAATCTTTCTACGGGTTTGTTTAGTAGTTTTGAATTTTTAAACTGCCTGTCTGTTTGTTCCTGATATGTATCTATGTACATGTGGGTTTAACATGTGTATAAATGGATATTGGTGCTTCTTTGCTGAAAACATGTCTTATGATTTAGCTGAGGAATTATACTGAAGTATGTGGTATTATCTTGCAGTCAAGCTGAATGCAAATCGCATGATTGTTGGTACTCTCCGTGGCTTCGACCAATTTATGAATTTGGTTGTTGACAATACTGTGGAGGTGAATGGCAATGAGAAAAATGACATAGGGATGGTGGTAAGTCCTCATACTATGCATTATCAAGTTATAATATTAGTAGTTTATGTATAGCATCTTATAGAGCTTGAGCTAGGTTTTATATTTGTTAATTTTCACTCGCTTTCTGCAGGTGATCAGAGGAAATAGTGTGGTCACTGTAGAGGCGCTTGAACCAGTGAACAGAAGCATCTAA

Protein sequence

>MS.gene007200.t1

MSRSGQPPDLKKYMDKQLQIKLNANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIGMVVIRGNSVVTVEALEPVNRSI