Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29881.t1 | XP_003599832.1 | 91.9 | 173 | 14 | 0 | 3 | 175 | 8 | 180 | 4.80E-79 | 303.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29881.t1 | G7IXV5 | 91.9 | 173 | 14 | 0 | 3 | 175 | 8 | 180 | 3.5e-79 | 303.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene29881 | 0.816503 | 5.35E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene29881 | 0.830274 | 3.22E-55 | -1.69E-46 |
| MS.gene049435 | MS.gene29881 | 0.826781 | 2.25E-54 | -1.69E-46 |
| MS.gene049821 | MS.gene29881 | 0.824467 | 7.95E-54 | -1.69E-46 |
| MS.gene049850 | MS.gene29881 | 0.804977 | 1.67E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene29881 | 0.80583 | 1.10E-49 | -1.69E-46 |
| MS.gene050237 | MS.gene29881 | 0.806983 | 6.31E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene29881 | 0.821887 | 3.18E-53 | -1.69E-46 |
| MS.gene050561 | MS.gene29881 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene29881 | 0.809076 | 2.26E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene29881 | 0.807033 | 6.16E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene29881 | 0.819115 | 1.38E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene29881 | 0.827862 | 1.24E-54 | -1.69E-46 |
| MS.gene051181 | MS.gene29881 | 0.813996 | 1.93E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene29881 | 0.805993 | 1.02E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene29881 | 0.806465 | 8.12E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene29881 | 0.803332 | 3.67E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene29881 | 0.815767 | 7.81E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene29881 | 0.80501 | 1.64E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene29881 | 0.808989 | 2.36E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene29881 | 0.81314 | 2.98E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene29881 | 0.807804 | 4.23E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene29881 | 0.801336 | 9.45E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene29881 | 0.808274 | 3.36E-50 | -1.69E-46 |
| MS.gene053883 | MS.gene29881 | 0.810203 | 1.30E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene29881 | 0.80394 | 2.74E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene29881 | 0.847579 | 1.06E-59 | -1.69E-46 |
| MS.gene054405 | MS.gene29881 | 0.819417 | 1.17E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene29881 | 0.824522 | 7.71E-54 | -1.69E-46 |
| MS.gene055227 | MS.gene29881 | 0.810093 | 1.37E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene29881 | 0.803601 | 3.22E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene29881 | 0.80059 | 1.34E-48 | -1.69E-46 |
| MS.gene055778 | MS.gene29881 | 0.806833 | 6.79E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene29881 | 0.842911 | 1.94E-58 | -1.69E-46 |
| MS.gene055926 | MS.gene29881 | 0.800456 | 1.43E-48 | -1.69E-46 |
| MS.gene05604 | MS.gene29881 | 0.820673 | 6.06E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene29881 | 0.808874 | 2.50E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene29881 | 0.804499 | 2.10E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene29881 | 0.859084 | 5.28E-63 | -1.69E-46 |
| MS.gene056498 | MS.gene29881 | 0.816386 | 5.68E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene29881 | 0.807759 | 4.32E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene29881 | 0.807124 | 5.89E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene29881 | 0.830734 | 2.49E-55 | -1.69E-46 |
| MS.gene056873 | MS.gene29881 | 0.82648 | 2.65E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene29881 | 0.832777 | 7.79E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene29881 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene057314 | MS.gene29881 | 0.823184 | 1.59E-53 | -1.69E-46 |
| MS.gene057414 | MS.gene29881 | 0.803783 | 2.96E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene29881 | 0.846971 | 1.55E-59 | -1.69E-46 |
| MS.gene057598 | MS.gene29881 | 0.805207 | 1.49E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene29881 | 0.805219 | 1.48E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene29881 | 0.803716 | 3.05E-49 | -1.69E-46 |
| MS.gene058009 | MS.gene29881 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene29881 | 0.811098 | 8.30E-51 | -1.69E-46 |
| MS.gene05847 | MS.gene29881 | 0.817589 | 3.05E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene29881 | 0.812995 | 3.20E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene29881 | 0.841031 | 6.08E-58 | -1.69E-46 |
| MS.gene059842 | MS.gene29881 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene29881 | 0.817306 | 3.53E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene29881 | 0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene29881 | 0.830313 | 3.15E-55 | -1.69E-46 |
| MS.gene06052 | MS.gene29881 | 0.805234 | 1.47E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene29881 | 0.816906 | 4.34E-52 | -1.69E-46 |
| MS.gene060634 | MS.gene29881 | -0.820261 | 7.53E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene29881 | 0.812244 | 4.67E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29881.t1 | MTR_3g047590 | 91.908 | 173 | 14 | 0 | 3 | 175 | 8 | 180 | 1.29e-114 | 323 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29881.t1 | AT1G06500 | 40.132 | 152 | 69 | 4 | 22 | 173 | 2 | 131 | 1.64e-29 | 105 |
| MS.gene29881.t1 | AT1G06500 | 40.132 | 152 | 69 | 4 | 22 | 173 | 2 | 131 | 1.64e-29 | 105 |
| MS.gene29881.t1 | AT1G06500 | 40.132 | 152 | 69 | 4 | 22 | 173 | 2 | 131 | 1.64e-29 | 105 |
| MS.gene29881.t1 | AT1G06500 | 40.132 | 152 | 69 | 4 | 22 | 173 | 2 | 131 | 1.64e-29 | 105 |
Find 47 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTGCTCCATCAAATATT+TGG | 0.194483 | 3.3:+38371513 | MS.gene29881:CDS |
| ACATTGGATGAATTTAAATA+TGG | 0.196305 | 3.3:+38371198 | MS.gene29881:CDS |
| CATGCATCCATCCATGATTT+TGG | 0.208551 | 3.3:-38371546 | None:intergenic |
| TTCCTTGTTGATGTGAATTC+TGG | 0.218734 | 3.3:-38371110 | None:intergenic |
| GCCTCTCCTCCGCTTTCTTC+TGG | 0.239178 | 3.3:-38371321 | None:intergenic |
| GGAAGGGAAACATTTAAATT+AGG | 0.257008 | 3.3:+38371444 | MS.gene29881:CDS |
| TATCGACTACTTCAAATAAA+TGG | 0.302615 | 3.3:+38371238 | MS.gene29881:CDS |
| ACACCGTGTTCGGAAAATTC+TGG | 0.307317 | 3.3:-38371158 | None:intergenic |
| ATCCAATGTTGTCATTCCTT+TGG | 0.364028 | 3.3:-38371184 | None:intergenic |
| CATTGGATGAATTTAAATAT+GGG | 0.366867 | 3.3:+38371199 | MS.gene29881:CDS |
| TGAAGTAGTCGATAAGCTTT+CGG | 0.377284 | 3.3:-38371229 | None:intergenic |
| GATCTTCATTGCCAAAATCA+TGG | 0.401657 | 3.3:+38371535 | MS.gene29881:CDS |
| CCTTTGGCTAACACCGTGTT+CGG | 0.405151 | 3.3:-38371168 | None:intergenic |
| CACCGTGTTCGGAAAATTCT+GGG | 0.447219 | 3.3:-38371157 | None:intergenic |
| ATGAAGATCCAAATATTTGA+TGG | 0.453836 | 3.3:-38371521 | None:intergenic |
| GTTGACTCTTGCCTCCGTCT+TGG | 0.455211 | 3.3:-38371299 | None:intergenic |
| CGGAGGAGAGGCAGAGAAGA+AGG | 0.461047 | 3.3:+38371332 | MS.gene29881:CDS |
| GTGTAAACAAGCTAGGCAAA+AGG | 0.464270 | 3.3:+38371484 | MS.gene29881:CDS |
| AAGAGCTGTAGAAGAAGGAA+GGG | 0.472950 | 3.3:+38371428 | MS.gene29881:CDS |
| AAAGAGCTGTAGAAGAAGGA+AGG | 0.481553 | 3.3:+38371427 | MS.gene29881:CDS |
| AGGAAAAGAGCTGTAGAAGA+AGG | 0.487958 | 3.3:+38371423 | MS.gene29881:CDS |
| TTAGGTGTTCTCCGAGGCTA+TGG | 0.498800 | 3.3:+38371462 | MS.gene29881:CDS |
| GACAAAAGCACAAGGTGTTA+CGG | 0.499816 | 3.3:+38371083 | MS.gene29881:CDS |
| GACTACTTCAAATAAATGGT+GGG | 0.503994 | 3.3:+38371242 | MS.gene29881:CDS |
| GAGAAGAAGGAATGTGAAAC+CGG | 0.508809 | 3.3:+38371345 | MS.gene29881:CDS |
| GGAAATAAAAGCGTTGAAAG+TGG | 0.510593 | 3.3:+38371129 | MS.gene29881:CDS |
| CTTCAAATAAATGGTGGGGA+TGG | 0.520192 | 3.3:+38371247 | MS.gene29881:CDS |
| TGTAAACAAGCTAGGCAAAA+GGG | 0.535406 | 3.3:+38371485 | MS.gene29881:CDS |
| ACCAGAAGAAAGCGGAGGAG+AGG | 0.544142 | 3.3:+38371320 | MS.gene29881:CDS |
| TTCATTGCCAAAATCATGGA+TGG | 0.547716 | 3.3:+38371539 | MS.gene29881:CDS |
| CGACTACTTCAAATAAATGG+TGG | 0.553601 | 3.3:+38371241 | MS.gene29881:CDS |
| CCGAACACGGTGTTAGCCAA+AGG | 0.554812 | 3.3:+38371168 | MS.gene29881:CDS |
| GCTTGTTTACACCATAGCCT+CGG | 0.602789 | 3.3:-38371473 | None:intergenic |
| GGCTATGGTGTAAACAAGCT+AGG | 0.604381 | 3.3:+38371477 | MS.gene29881:CDS |
| ACTACTTCAAATAAATGGTG+GGG | 0.619643 | 3.3:+38371243 | MS.gene29881:CDS |
| TGCAACAAGCAAATGATGGG+AGG | 0.634860 | 3.3:+38371061 | MS.gene29881:CDS |
| AGTCAACCAGAAGAAAGCGG+AGG | 0.636989 | 3.3:+38371315 | MS.gene29881:CDS |
| AAGAGTCAACCAGAAGAAAG+CGG | 0.639731 | 3.3:+38371312 | MS.gene29881:CDS |
| AGCCAAAGGAATGACAACAT+TGG | 0.640708 | 3.3:+38371182 | MS.gene29881:CDS |
| CACCAGAATTCACATCAACA+AGG | 0.644361 | 3.3:+38371108 | MS.gene29881:CDS |
| GTAGTCGATAAGCTTTCGGA+CGG | 0.669763 | 3.3:-38371225 | None:intergenic |
| CATCTTTGTTGAGATCTGTG+AGG | 0.675369 | 3.3:+38371403 | MS.gene29881:CDS |
| AGTAGTGAAGAAACTCGTCA+AGG | 0.678227 | 3.3:+38371378 | MS.gene29881:CDS |
| CGTACTGACACAAACCAAGA+CGG | 0.693330 | 3.3:+38371285 | MS.gene29881:CDS |
| GATGGGAGGACAAAAGCACA+AGG | 0.714490 | 3.3:+38371075 | MS.gene29881:CDS |
| TTTAAATTAGGTGTTCTCCG+AGG | 0.721425 | 3.3:+38371456 | MS.gene29881:CDS |
| ACTGACACAAACCAAGACGG+AGG | 0.740979 | 3.3:+38371288 | MS.gene29881:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATTGGATGAATTTAAATA+TGG | + | chr3.3:38371198-38371217 | MS.gene29881:CDS | 20.0% |
| !! | CATTGGATGAATTTAAATAT+GGG | + | chr3.3:38371199-38371218 | MS.gene29881:CDS | 20.0% |
| ! | ATATTGCTCCATCAAATATT+TGG | + | chr3.3:38371513-38371532 | MS.gene29881:CDS | 25.0% |
| ! | ATGAAGATCCAAATATTTGA+TGG | - | chr3.3:38371524-38371543 | None:intergenic | 25.0% |
| !! | TATCGACTACTTCAAATAAA+TGG | + | chr3.3:38371238-38371257 | MS.gene29881:CDS | 25.0% |
| ACTACTTCAAATAAATGGTG+GGG | + | chr3.3:38371243-38371262 | MS.gene29881:CDS | 30.0% | |
| GACTACTTCAAATAAATGGT+GGG | + | chr3.3:38371242-38371261 | MS.gene29881:CDS | 30.0% | |
| GGAAGGGAAACATTTAAATT+AGG | + | chr3.3:38371444-38371463 | MS.gene29881:CDS | 30.0% | |
| ! | TTTTGCAACAAGCAAATGAT+GGG | + | chr3.3:38371058-38371077 | MS.gene29881:CDS | 30.0% |
| ATCCAATGTTGTCATTCCTT+TGG | - | chr3.3:38371187-38371206 | None:intergenic | 35.0% | |
| CGACTACTTCAAATAAATGG+TGG | + | chr3.3:38371241-38371260 | MS.gene29881:CDS | 35.0% | |
| GATCTTCATTGCCAAAATCA+TGG | + | chr3.3:38371535-38371554 | MS.gene29881:CDS | 35.0% | |
| TGAAGTAGTCGATAAGCTTT+CGG | - | chr3.3:38371232-38371251 | None:intergenic | 35.0% | |
| TGTAAACAAGCTAGGCAAAA+GGG | + | chr3.3:38371485-38371504 | MS.gene29881:CDS | 35.0% | |
| TTCATTGCCAAAATCATGGA+TGG | + | chr3.3:38371539-38371558 | MS.gene29881:CDS | 35.0% | |
| TTCCTTGTTGATGTGAATTC+TGG | - | chr3.3:38371113-38371132 | None:intergenic | 35.0% | |
| TTTAAATTAGGTGTTCTCCG+AGG | + | chr3.3:38371456-38371475 | MS.gene29881:CDS | 35.0% | |
| ! | GTTTTGCAACAAGCAAATGA+TGG | + | chr3.3:38371057-38371076 | MS.gene29881:CDS | 35.0% |
| ! | TTCACTACTTTCAGCTTTTC+CGG | - | chr3.3:38371367-38371386 | None:intergenic | 35.0% |
| !! | GGAAATAAAAGCGTTGAAAG+TGG | + | chr3.3:38371129-38371148 | MS.gene29881:CDS | 35.0% |
| AAAGAGCTGTAGAAGAAGGA+AGG | + | chr3.3:38371427-38371446 | MS.gene29881:CDS | 40.0% | |
| AAGAGCTGTAGAAGAAGGAA+GGG | + | chr3.3:38371428-38371447 | MS.gene29881:CDS | 40.0% | |
| AAGAGTCAACCAGAAGAAAG+CGG | + | chr3.3:38371312-38371331 | MS.gene29881:CDS | 40.0% | |
| AGCCAAAGGAATGACAACAT+TGG | + | chr3.3:38371182-38371201 | MS.gene29881:CDS | 40.0% | |
| AGGAAAAGAGCTGTAGAAGA+AGG | + | chr3.3:38371423-38371442 | MS.gene29881:CDS | 40.0% | |
| AGTAGTGAAGAAACTCGTCA+AGG | + | chr3.3:38371378-38371397 | MS.gene29881:CDS | 40.0% | |
| CACCAGAATTCACATCAACA+AGG | + | chr3.3:38371108-38371127 | MS.gene29881:CDS | 40.0% | |
| CATCTTTGTTGAGATCTGTG+AGG | + | chr3.3:38371403-38371422 | MS.gene29881:CDS | 40.0% | |
| GAGAAGAAGGAATGTGAAAC+CGG | + | chr3.3:38371345-38371364 | MS.gene29881:CDS | 40.0% | |
| GTGTAAACAAGCTAGGCAAA+AGG | + | chr3.3:38371484-38371503 | MS.gene29881:CDS | 40.0% | |
| ! | CATGCATCCATCCATGATTT+TGG | - | chr3.3:38371549-38371568 | None:intergenic | 40.0% |
| ! | CTTCAAATAAATGGTGGGGA+TGG | + | chr3.3:38371247-38371266 | MS.gene29881:CDS | 40.0% |
| ! | GACAAAAGCACAAGGTGTTA+CGG | + | chr3.3:38371083-38371102 | MS.gene29881:CDS | 40.0% |
| ACACCGTGTTCGGAAAATTC+TGG | - | chr3.3:38371161-38371180 | None:intergenic | 45.0% | |
| CACCGTGTTCGGAAAATTCT+GGG | - | chr3.3:38371160-38371179 | None:intergenic | 45.0% | |
| GCTTGTTTACACCATAGCCT+CGG | - | chr3.3:38371476-38371495 | None:intergenic | 45.0% | |
| GGCTATGGTGTAAACAAGCT+AGG | + | chr3.3:38371477-38371496 | MS.gene29881:CDS | 45.0% | |
| GTAGTCGATAAGCTTTCGGA+CGG | - | chr3.3:38371228-38371247 | None:intergenic | 45.0% | |
| ! | CGTACTGACACAAACCAAGA+CGG | + | chr3.3:38371285-38371304 | MS.gene29881:CDS | 45.0% |
| ! | TGCAACAAGCAAATGATGGG+AGG | + | chr3.3:38371061-38371080 | MS.gene29881:CDS | 45.0% |
| ACTGACACAAACCAAGACGG+AGG | + | chr3.3:38371288-38371307 | MS.gene29881:CDS | 50.0% | |
| AGTCAACCAGAAGAAAGCGG+AGG | + | chr3.3:38371315-38371334 | MS.gene29881:CDS | 50.0% | |
| CCTTTGGCTAACACCGTGTT+CGG | - | chr3.3:38371171-38371190 | None:intergenic | 50.0% | |
| TTAGGTGTTCTCCGAGGCTA+TGG | + | chr3.3:38371462-38371481 | MS.gene29881:CDS | 50.0% | |
| ! | CGCCCAGAATTTTCCGAACA+CGG | + | chr3.3:38371155-38371174 | MS.gene29881:CDS | 50.0% |
| ! | GATGGGAGGACAAAAGCACA+AGG | + | chr3.3:38371075-38371094 | MS.gene29881:CDS | 50.0% |
| ACCAGAAGAAAGCGGAGGAG+AGG | + | chr3.3:38371320-38371339 | MS.gene29881:CDS | 55.0% | |
| ! | CCGAACACGGTGTTAGCCAA+AGG | + | chr3.3:38371168-38371187 | MS.gene29881:CDS | 55.0% |
| ! | GTTGACTCTTGCCTCCGTCT+TGG | - | chr3.3:38371302-38371321 | None:intergenic | 55.0% |
| CGGAGGAGAGGCAGAGAAGA+AGG | + | chr3.3:38371332-38371351 | MS.gene29881:CDS | 60.0% | |
| GCCTCTCCTCCGCTTTCTTC+TGG | - | chr3.3:38371324-38371343 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 38371042 | 38371569 | 38371042 | ID=MS.gene29881 |
| chr3.3 | mRNA | 38371042 | 38371569 | 38371042 | ID=MS.gene29881.t1;Parent=MS.gene29881 |
| chr3.3 | exon | 38371042 | 38371569 | 38371042 | ID=MS.gene29881.t1.exon1;Parent=MS.gene29881.t1 |
| chr3.3 | CDS | 38371042 | 38371569 | 38371042 | ID=cds.MS.gene29881.t1;Parent=MS.gene29881.t1 |
| Gene Sequence |
| Protein sequence |
