AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35064

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35064.t1 MTR_1g115920 93.103 464 28 2 118 578 372 834 0.0 801
MS.gene35064.t1 MTR_1g115920 90.909 66 3 2 3 66 82 146 3.68e-25 110
MS.gene35064.t1 MTR_1g115920 68.421 76 19 2 65 135 241 316 1.01e-13 74.7
MS.gene35064.t1 MTR_4g094355 81.139 281 18 3 25 305 6 251 2.58e-140 414
MS.gene35064.t1 MTR_4g094355 92.147 191 15 0 388 578 250 440 8.44e-122 367
MS.gene35064.t1 MTR_5g039050 84.753 223 25 3 268 490 67 280 4.49e-112 341
MS.gene35064.t1 MTR_5g039050 82.222 45 7 1 130 173 6 50 1.45e-14 76.3
MS.gene35064.t1 MTR_5g039100 75.000 68 13 2 2 66 38 104 2.29e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35064.t1 AT1G28060 66.811 461 146 4 118 575 330 786 0.0 605
MS.gene35064.t1 AT1G28060 66.811 461 146 4 118 575 330 786 0.0 605
MS.gene35064.t1 AT1G28060 66.811 461 146 4 118 575 330 786 0.0 605
MS.gene35064.t1 AT3G55930 62.222 270 98 3 305 572 3 270 5.97e-99 301

Find 144 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACACCAGAACACATTTAT+TGG 0.127318 4.4:-20404933 None:intergenic
ATAAAATTGAAGAGTAAATT+TGG 0.190886 4.4:+20404053 MS.gene35064:CDS
TCTCCTTAGCCATACGCTTT+TGG 0.207554 4.4:-20404421 None:intergenic
TGGATGATGATAAGAGAATT+AGG 0.216295 4.4:+20403496 MS.gene35064:CDS
TGCTGGGGTTCCTCATTATT+GGG 0.233213 4.4:+20405042 MS.gene35064:CDS
ATGCTGGGGTTCCTCATTAT+TGG 0.242299 4.4:+20405041 MS.gene35064:CDS
CTTCTGCATTCTGGAAATTA+TGG 0.253483 4.4:+20404236 MS.gene35064:CDS
ACACACTTGTCTCACTTTAC+AGG 0.257138 4.4:+20404693 MS.gene35064:CDS
ACAAGTGTGTCCAATGTATT+TGG 0.267435 4.4:-20404680 None:intergenic
CTCATCTTGACCTTTGGTTT+TGG 0.279974 4.4:-20404485 None:intergenic
CTTTGCTAGCTGTGCTTGTT+TGG 0.291419 4.4:-20404102 None:intergenic
TCCTGTTTAGTTGGCTTGAT+AGG 0.304342 4.4:-20404380 None:intergenic
CTCTGTAATTTCTATTAAGT+TGG 0.305002 4.4:-20404150 None:intergenic
ATTAAGTTGGGATTGATATC+AGG 0.316384 4.4:-20404137 None:intergenic
AAGCAACCTTAGCACATTAA+AGG 0.318390 4.4:+20403848 MS.gene35064:CDS
TTCTTCTGCTCCTGTTTAGT+TGG 0.318787 4.4:-20404389 None:intergenic
GGTGGAGGGGCGGGCTCAGC+AGG 0.333316 4.4:-20404350 None:intergenic
GACGTGGCACTTCTGCATTC+TGG 0.335270 4.4:+20404227 MS.gene35064:CDS
GAACCTATTGAAACTCGATT+TGG 0.351326 4.4:-20404969 None:intergenic
GCTCATCTATTTCACGTCCC+TGG 0.351440 4.4:-20403797 None:intergenic
TGAAATACTAAAACCTGTAT+TGG 0.355353 4.4:+20403902 MS.gene35064:CDS
AGATGAAGGCAATATGAAAA+TGG 0.363556 4.4:+20404283 MS.gene35064:CDS
GGATGATGATAAGAGAATTA+GGG 0.370764 4.4:+20403497 MS.gene35064:CDS
AAGAAGGAACGTAGGAGATT+TGG 0.374683 4.4:+20403531 MS.gene35064:CDS
AAATTACTCATCTTGACCTT+TGG 0.381357 4.4:-20404491 None:intergenic
AAGAGAAAATTCATCCTCTT+AGG 0.385406 4.4:-20403993 None:intergenic
TTGCCAAATCGAGTTTCAAT+AGG 0.388491 4.4:+20404966 MS.gene35064:CDS
TCTTTGTGGAGGAAGGAAAA+TGG 0.389531 4.4:+20404012 MS.gene35064:CDS
AGGTTGAGGTGGTGGAGGGG+CGG 0.390911 4.4:-20404360 None:intergenic
TCTGTAATTTCTATTAAGTT+GGG 0.393151 4.4:-20404149 None:intergenic
TTGATAGGTTGAGGTGGTGG+AGG 0.401991 4.4:-20404365 None:intergenic
AGCAAGTCAATTAAGAGGTA+TGG 0.404160 4.4:+20404836 MS.gene35064:CDS
TTCTTCTCACGCAGCTCAGC+AGG 0.405655 4.4:-20404635 None:intergenic
TCAAAATGAGGATTAGATTC+AGG 0.407433 4.4:-20403933 None:intergenic
TGCAGCTGCTGAGCGTGAGC+AGG 0.412035 4.4:+20404577 MS.gene35064:CDS
ACCTATCAAGCCAACTAAAC+AGG 0.413749 4.4:+20404379 MS.gene35064:CDS
TAGAGAACATAGCGAGGATC+AGG 0.414473 4.4:+20403470 MS.gene35064:CDS
GGTTGAGGTGGTGGAGGGGC+GGG 0.419674 4.4:-20404359 None:intergenic
CTCGATACTCCTCGCATAAG+AGG 0.421449 4.4:+20403442 MS.gene35064:CDS
TTATGCTTAGACGCATCAAT+TGG 0.421874 4.4:+20404864 MS.gene35064:CDS
GAATCTACAGCTATGTTTCC+TGG 0.422589 4.4:+20403705 MS.gene35064:CDS
TTTAATGTGCTAAGGTTGCT+TGG 0.430099 4.4:-20403846 None:intergenic
CACCACTCAATATCCGGAAT+CGG 0.431295 4.4:-20404203 None:intergenic
CTCATCTATTTCACGTCCCT+GGG 0.433089 4.4:-20403796 None:intergenic
GGCTCAGCAGGCGGTTCAAT+AGG 0.433119 4.4:-20404338 None:intergenic
TAACGTCGGGTGTAGCAATC+TGG 0.434175 4.4:-20403728 None:intergenic
ATAAACTGTAGAGCGGGCCT+TGG 0.434948 4.4:-20404735 None:intergenic
CAAAGCTCCTGCGGCCTAAG+AGG 0.436122 4.4:+20403979 MS.gene35064:CDS
AACTTTACGAGCAGCTGCTT+CGG 0.436123 4.4:-20405011 None:intergenic
ACATAGCGAGGATCAGGAAT+TGG 0.436226 4.4:+20403476 MS.gene35064:CDS
GGCTTGATAGGTTGAGGTGG+TGG 0.440923 4.4:-20404368 None:intergenic
TGTTCTTCGTCTTGACGCCC+AGG 0.441585 4.4:+20403779 MS.gene35064:CDS
CGATGCCAATAAATGTGTTC+TGG 0.441704 4.4:+20404928 MS.gene35064:CDS
GGAGGGGCGGGCTCAGCAGG+CGG 0.453459 4.4:-20404347 None:intergenic
AAAACTGAAGGACCCGATTC+CGG 0.455391 4.4:+20404190 MS.gene35064:CDS
AGTAATTTACATAAGGTACT+AGG 0.456676 4.4:+20404506 MS.gene35064:CDS
ACGTCCCACCACTCAATATC+CGG 0.458176 4.4:-20404209 None:intergenic
TAGGGTTTCTGAAGCAAAGA+AGG 0.462240 4.4:+20403515 MS.gene35064:CDS
ATTAGTGTTGTCGTGGTTGA+AGG 0.473394 4.4:+20404809 MS.gene35064:CDS
TGCTGAGCTGCGTGAGAAGA+AGG 0.474449 4.4:+20404637 MS.gene35064:CDS
AGATCCCACCCGGCTTGAAA+AGG 0.474598 4.4:+20404544 MS.gene35064:CDS
AGGAGATTTGGTGATAAACT+TGG 0.481118 4.4:+20403543 MS.gene35064:CDS
CACACTTGTCTCACTTTACA+GGG 0.483911 4.4:+20404694 MS.gene35064:CDS
GTGAGCAGGCTCACATAGAT+AGG 0.485650 4.4:+20404591 MS.gene35064:CDS
GCTCAACAAAATCGTTTGAC+TGG 0.491510 4.4:+20404764 MS.gene35064:CDS
TATTGACCTTTAATGTGCTA+AGG 0.494808 4.4:-20403854 None:intergenic
CAAGATGAGTAATTTACATA+AGG 0.497213 4.4:+20404499 MS.gene35064:CDS
AACAGGAGCAGAAGAAACTA+AGG 0.497775 4.4:+20404396 MS.gene35064:CDS
TGGGCGTCAAGACGAAGAAC+AGG 0.500740 4.4:-20403777 None:intergenic
TCAGGATCGACATCCAATAC+AGG 0.501479 4.4:-20403915 None:intergenic
GCCTTCATCTATAGTACCGT+TGG 0.504964 4.4:-20404270 None:intergenic
GGAGCCTTGGTTGGTTTCTG+AGG 0.506975 4.4:-20403756 None:intergenic
TGCCTTAGCAAAGGCTAAGA+AGG 0.507867 4.4:+20403584 MS.gene35064:CDS
TGATAGGTTGAGGTGGTGGA+GGG 0.509364 4.4:-20404364 None:intergenic
ACCACTCAATATCCGGAATC+GGG 0.511345 4.4:-20404202 None:intergenic
GCAAGTCAATTAAGAGGTAT+GGG 0.512247 4.4:+20404837 MS.gene35064:CDS
CAAGTGTGTCCAATGTATTT+GGG 0.514012 4.4:-20404679 None:intergenic
TAAACTGTAGAGCGGGCCTT+GGG 0.515526 4.4:-20404734 None:intergenic
CGAAGAACAGGAGCCTTGGT+TGG 0.517067 4.4:-20403765 None:intergenic
ACTTTCATGTGGAAATTCCT+CGG 0.518196 4.4:+20404315 MS.gene35064:CDS
GTGGAAGCAAGTCAATTAAG+AGG 0.522404 4.4:+20404831 MS.gene35064:CDS
GACCCAAAAGCGTATGGCTA+AGG 0.527773 4.4:+20404418 MS.gene35064:CDS
ATCGAAGCGCGATGAAGAGA+GGG 0.529353 4.4:+20403413 MS.gene35064:CDS
GGCTAAGGAGAAAGCACGAC+AGG 0.533388 4.4:+20404433 MS.gene35064:CDS
GAGCCTTGGTTGGTTTCTGA+GGG 0.533893 4.4:-20403755 None:intergenic
AGCTCAAGCAAAAGAGCAGA+AGG 0.535132 4.4:+20404079 MS.gene35064:CDS
GGATATTGAGTGGTGGGACG+TGG 0.536150 4.4:+20404211 MS.gene35064:CDS
GTTTCATGACCCAAATACAT+TGG 0.539983 4.4:+20404670 MS.gene35064:CDS
AGCTGCGTGAGAAGAAGGAA+AGG 0.547194 4.4:+20404642 MS.gene35064:CDS
CCCATTCTCTCATCAAAATG+AGG 0.549338 4.4:-20403945 None:intergenic
CAAACAAGCACAGCTAGCAA+AGG 0.555686 4.4:+20404103 MS.gene35064:CDS
TTCTGAGGGACAGTAACGTC+GGG 0.556866 4.4:-20403741 None:intergenic
AAGACGAAGAACAGGAGCCT+TGG 0.557281 4.4:-20403769 None:intergenic
TTCATCTATAGTACCGTTGG+CGG 0.558630 4.4:-20404267 None:intergenic
AATCGAAGCGCGATGAAGAG+AGG 0.561052 4.4:+20403412 MS.gene35064:CDS
GACGAAAGAGAGGGTAGCAA+AGG 0.561832 4.4:+20403381 MS.gene35064:CDS
GTTCTTCGTCTTGACGCCCA+GGG 0.562372 4.4:+20403780 MS.gene35064:CDS
TCGGGTGTAGCAATCTGGCC+AGG 0.563408 4.4:-20403723 None:intergenic
AATTCATCCTCTTAGGCCGC+AGG 0.563858 4.4:-20403986 None:intergenic
GGAAGAATTCAACTTTCATG+TGG 0.569338 4.4:+20404304 MS.gene35064:CDS
AGTGTTGTCGTGGTTGAAGG+TGG 0.570940 4.4:+20404812 MS.gene35064:CDS
TTAGTTGGCTTGATAGGTTG+AGG 0.576255 4.4:-20404374 None:intergenic
ATTAACATAAACTGTAGAGC+GGG 0.580679 4.4:-20404741 None:intergenic
GTAATTTACATAAGGTACTA+GGG 0.581923 4.4:+20404507 MS.gene35064:CDS
AGCCTTCTTAGCCTTTGCTA+AGG 0.581971 4.4:-20403586 None:intergenic
GATAGGTTGAGGTGGTGGAG+GGG 0.582077 4.4:-20404363 None:intergenic
GTTGGCTTGATAGGTTGAGG+TGG 0.586185 4.4:-20404371 None:intergenic
GCCAACGGTACTATAGATGA+AGG 0.586217 4.4:+20404269 MS.gene35064:CDS
CTAAGGCATCACTACTAGAG+AGG 0.588190 4.4:-20403569 None:intergenic
AAAGAGAGGGTAGCAAAGGT+AGG 0.594676 4.4:+20403385 MS.gene35064:CDS
TATGGTGACATTGCCGCCAA+CGG 0.595136 4.4:+20404254 MS.gene35064:CDS
ACTAAGGACCCAAAAGCGTA+TGG 0.600708 4.4:+20404412 MS.gene35064:CDS
TGATGGTATTAGTGTTGTCG+TGG 0.603393 4.4:+20404802 MS.gene35064:CDS
TCTATCTTTCCTCTTATGCG+AGG 0.606208 4.4:-20403451 None:intergenic
GACAGGGATATGGGATACGA+TGG 0.607091 4.4:+20403357 None:intergenic
CATTTGTATAGTTGACAGCA+TGG 0.608274 4.4:-20405066 None:intergenic
CGACAGGAGATGATTAGAGA+GGG 0.610590 4.4:+20404449 MS.gene35064:CDS
CGATTCCGGATATTGAGTGG+TGG 0.614030 4.4:+20404204 MS.gene35064:CDS
AAATGACTTCTCTCATCCCA+AGG 0.614270 4.4:+20404718 MS.gene35064:CDS
GGATGTGCGGTGATTTGTGA+TGG 0.616908 4.4:+20404785 MS.gene35064:CDS
TGTAATGAAAGCAAAACTGA+AGG 0.617069 4.4:+20404178 MS.gene35064:CDS
ACAGCATGGTCCCAATAATG+AGG 0.617442 4.4:-20405052 None:intergenic
GAATTTGAGTTATTCAACTG+AGG 0.620565 4.4:-20403651 None:intergenic
TAGTAGTGATGCCTTAGCAA+AGG 0.620911 4.4:+20403575 MS.gene35064:CDS
AGCACAGCTAGCAAAGGCAA+AGG 0.626359 4.4:+20404109 MS.gene35064:CDS
GGACGTGAAATAGATGAGCA+TGG 0.626376 4.4:+20403801 MS.gene35064:CDS
ATACGATGGAGACGAAAGAG+AGG 0.628066 4.4:+20403371 MS.gene35064:CDS
GCAGGCGGTTCAATAGGCCG+AGG 0.628346 4.4:-20404332 None:intergenic
CATAGAACCGCCAAAACCAA+AGG 0.629032 4.4:+20404475 MS.gene35064:CDS
CCAATAAATGTGTTCTGGTG+TGG 0.635559 4.4:+20404933 MS.gene35064:CDS
ACGACAGGAGATGATTAGAG+AGG 0.638900 4.4:+20404448 MS.gene35064:CDS
TTTCTGAGGGACAGTAACGT+CGG 0.642738 4.4:-20403742 None:intergenic
GATTCCGGATATTGAGTGGT+GGG 0.645660 4.4:+20404205 MS.gene35064:CDS
ACCCGATTCCGGATATTGAG+TGG 0.647460 4.4:+20404201 MS.gene35064:CDS
TCAACAAAACAAAGCTCCTG+CGG 0.648319 4.4:+20403970 MS.gene35064:CDS
GAAAGATAGAGAACATAGCG+AGG 0.651265 4.4:+20403464 MS.gene35064:CDS
AAATGTGTTCTGGTGTGGCA+AGG 0.657323 4.4:+20404938 MS.gene35064:CDS
GAAGCGCGATGAAGAGAGGG+AGG 0.664190 4.4:+20403416 MS.gene35064:CDS
TACGATGGAGACGAAAGAGA+GGG 0.671943 4.4:+20403372 MS.gene35064:CDS
CTGAAGCAAAGAAGGAACGT+AGG 0.673233 4.4:+20403523 MS.gene35064:CDS
AAATCGTTTGACTGGATGTG+CGG 0.693768 4.4:+20404772 MS.gene35064:CDS
AAGCAACTCAAGATCCCACC+CGG 0.703568 4.4:+20404534 MS.gene35064:CDS
CATTAACATAAACTGTAGAG+CGG 0.704797 4.4:-20404742 None:intergenic
TGTCCCTCAGAAACCAACCA+AGG 0.741969 4.4:+20403752 MS.gene35064:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTGAAGAGTAAATT+TGG + chr4.4:20404053-20404072 MS.gene35064:CDS 15.0%
!! TCTGTAATTTCTATTAAGTT+GGG - chr4.4:20404152-20404171 None:intergenic 20.0%
! CAAGATGAGTAATTTACATA+AGG + chr4.4:20404499-20404518 MS.gene35064:CDS 25.0%
! CTCTGTAATTTCTATTAAGT+TGG - chr4.4:20404153-20404172 None:intergenic 25.0%
! TGAAATACTAAAACCTGTAT+TGG + chr4.4:20403902-20403921 MS.gene35064:CDS 25.0%
!! AGTAATTTACATAAGGTACT+AGG + chr4.4:20404506-20404525 MS.gene35064:CDS 25.0%
!! GTAATTTACATAAGGTACTA+GGG + chr4.4:20404507-20404526 MS.gene35064:CDS 25.0%
AAATTACTCATCTTGACCTT+TGG - chr4.4:20404494-20404513 None:intergenic 30.0%
AAGAGAAAATTCATCCTCTT+AGG - chr4.4:20403996-20404015 None:intergenic 30.0%
AGATGAAGGCAATATGAAAA+TGG + chr4.4:20404283-20404302 MS.gene35064:CDS 30.0%
ATTAACATAAACTGTAGAGC+GGG - chr4.4:20404744-20404763 None:intergenic 30.0%
CATTAACATAAACTGTAGAG+CGG - chr4.4:20404745-20404764 None:intergenic 30.0%
GGATGATGATAAGAGAATTA+GGG + chr4.4:20403497-20403516 MS.gene35064:CDS 30.0%
TATTGACCTTTAATGTGCTA+AGG - chr4.4:20403857-20403876 None:intergenic 30.0%
TCAAAATGAGGATTAGATTC+AGG - chr4.4:20403936-20403955 None:intergenic 30.0%
TGGATGATGATAAGAGAATT+AGG + chr4.4:20403496-20403515 MS.gene35064:CDS 30.0%
TGTAATGAAAGCAAAACTGA+AGG + chr4.4:20404178-20404197 MS.gene35064:CDS 30.0%
! GAATTTGAGTTATTCAACTG+AGG - chr4.4:20403654-20403673 None:intergenic 30.0%
!! ATTAAGTTGGGATTGATATC+AGG - chr4.4:20404140-20404159 None:intergenic 30.0%
AAGCAACCTTAGCACATTAA+AGG + chr4.4:20403848-20403867 MS.gene35064:CDS 35.0%
ACAAGTGTGTCCAATGTATT+TGG - chr4.4:20404683-20404702 None:intergenic 35.0%
ACTTTCATGTGGAAATTCCT+CGG + chr4.4:20404315-20404334 MS.gene35064:CDS 35.0%
AGCAAGTCAATTAAGAGGTA+TGG + chr4.4:20404836-20404855 MS.gene35064:CDS 35.0%
CAAGTGTGTCCAATGTATTT+GGG - chr4.4:20404682-20404701 None:intergenic 35.0%
CATTTGTATAGTTGACAGCA+TGG - chr4.4:20405069-20405088 None:intergenic 35.0%
GAACCTATTGAAACTCGATT+TGG - chr4.4:20404972-20404991 None:intergenic 35.0%
GGAAGAATTCAACTTTCATG+TGG + chr4.4:20404304-20404323 MS.gene35064:CDS 35.0%
GTTTCATGACCCAAATACAT+TGG + chr4.4:20404670-20404689 MS.gene35064:CDS 35.0%
TTATGCTTAGACGCATCAAT+TGG + chr4.4:20404864-20404883 MS.gene35064:CDS 35.0%
TTGCCAAATCGAGTTTCAAT+AGG + chr4.4:20404966-20404985 MS.gene35064:CDS 35.0%
TTTAATGTGCTAAGGTTGCT+TGG - chr4.4:20403849-20403868 None:intergenic 35.0%
! AATTTTCTCTTTGTGGAGGA+AGG + chr4.4:20404005-20404024 MS.gene35064:CDS 35.0%
! CTTCTGCATTCTGGAAATTA+TGG + chr4.4:20404236-20404255 MS.gene35064:CDS 35.0%
! GAGGATGAATTTTCTCTTTG+TGG + chr4.4:20403998-20404017 MS.gene35064:CDS 35.0%
! GATGAATTTTCTCTTTGTGG+AGG + chr4.4:20404001-20404020 MS.gene35064:CDS 35.0%
! GCAAGTCAATTAAGAGGTAT+GGG + chr4.4:20404837-20404856 MS.gene35064:CDS 35.0%
! GTAAAGTTTTTGTGGATGCT+GGG + chr4.4:20405026-20405045 MS.gene35064:CDS 35.0%
! TAAAGTTTTTGTGGATGCTG+GGG + chr4.4:20405027-20405046 MS.gene35064:CDS 35.0%
!! AGGAGATTTGGTGATAAACT+TGG + chr4.4:20403543-20403562 MS.gene35064:CDS 35.0%
!! CCTCATTTTGATGAGAGAAT+GGG + chr4.4:20403945-20403964 MS.gene35064:CDS 35.0%
!! TCCTCATTTTGATGAGAGAA+TGG + chr4.4:20403944-20403963 MS.gene35064:CDS 35.0%
AAATCGTTTGACTGGATGTG+CGG + chr4.4:20404772-20404791 MS.gene35064:CDS 40.0%
AAATGACTTCTCTCATCCCA+AGG + chr4.4:20404718-20404737 MS.gene35064:CDS 40.0%
AACAGGAGCAGAAGAAACTA+AGG + chr4.4:20404396-20404415 MS.gene35064:CDS 40.0%
AAGAAGGAACGTAGGAGATT+TGG + chr4.4:20403531-20403550 MS.gene35064:CDS 40.0%
ACACACTTGTCTCACTTTAC+AGG + chr4.4:20404693-20404712 MS.gene35064:CDS 40.0%
ACCTATCAAGCCAACTAAAC+AGG + chr4.4:20404379-20404398 MS.gene35064:CDS 40.0%
ATTAGTGTTGTCGTGGTTGA+AGG + chr4.4:20404809-20404828 MS.gene35064:CDS 40.0%
CACACTTGTCTCACTTTACA+GGG + chr4.4:20404694-20404713 MS.gene35064:CDS 40.0%
CCAATAAATGTGTTCTGGTG+TGG + chr4.4:20404933-20404952 MS.gene35064:CDS 40.0%
CCACACCAGAACACATTTAT+TGG - chr4.4:20404936-20404955 None:intergenic 40.0%
CCCATTCTCTCATCAAAATG+AGG - chr4.4:20403948-20403967 None:intergenic 40.0%
CGATGCCAATAAATGTGTTC+TGG + chr4.4:20404928-20404947 MS.gene35064:CDS 40.0%
GAAAGATAGAGAACATAGCG+AGG + chr4.4:20403464-20403483 MS.gene35064:CDS 40.0%
GAATCTACAGCTATGTTTCC+TGG + chr4.4:20403705-20403724 MS.gene35064:CDS 40.0%
GCTCAACAAAATCGTTTGAC+TGG + chr4.4:20404764-20404783 MS.gene35064:CDS 40.0%
GTGGAAGCAAGTCAATTAAG+AGG + chr4.4:20404831-20404850 MS.gene35064:CDS 40.0%
TAGTAGTGATGCCTTAGCAA+AGG + chr4.4:20403575-20403594 MS.gene35064:CDS 40.0%
TCAACAAAACAAAGCTCCTG+CGG + chr4.4:20403970-20403989 MS.gene35064:CDS 40.0%
TCTATCTTTCCTCTTATGCG+AGG - chr4.4:20403454-20403473 None:intergenic 40.0%
TCTTTGTGGAGGAAGGAAAA+TGG + chr4.4:20404012-20404031 MS.gene35064:CDS 40.0%
TGATGGTATTAGTGTTGTCG+TGG + chr4.4:20404802-20404821 MS.gene35064:CDS 40.0%
TTCATCTATAGTACCGTTGG+CGG - chr4.4:20404270-20404289 None:intergenic 40.0%
TTCTTCTGCTCCTGTTTAGT+TGG - chr4.4:20404392-20404411 None:intergenic 40.0%
! AGCTGCTCGTAAAGTTTTTG+TGG + chr4.4:20405018-20405037 MS.gene35064:CDS 40.0%
! CGTAAAGTTTTTGTGGATGC+TGG + chr4.4:20405025-20405044 MS.gene35064:CDS 40.0%
!! CTCATCTTGACCTTTGGTTT+TGG - chr4.4:20404488-20404507 None:intergenic 40.0%
!! TACGTACTTCCTTTTCAAGC+CGG - chr4.4:20404556-20404575 None:intergenic 40.0%
!! TAGGGTTTCTGAAGCAAAGA+AGG + chr4.4:20403515-20403534 MS.gene35064:CDS 40.0%
!! TCCTGTTTAGTTGGCTTGAT+AGG - chr4.4:20404383-20404402 None:intergenic 40.0%
!! TTAGTTGGCTTGATAGGTTG+AGG - chr4.4:20404377-20404396 None:intergenic 40.0%
!!! ATCTTGACCTTTGGTTTTGG+CGG - chr4.4:20404485-20404504 None:intergenic 40.0%
AAAACTGAAGGACCCGATTC+CGG + chr4.4:20404190-20404209 MS.gene35064:CDS 45.0%
AAAGAGAGGGTAGCAAAGGT+AGG + chr4.4:20403385-20403404 MS.gene35064:CDS 45.0%
AAATGTGTTCTGGTGTGGCA+AGG + chr4.4:20404938-20404957 MS.gene35064:CDS 45.0%
AACTTTACGAGCAGCTGCTT+CGG - chr4.4:20405014-20405033 None:intergenic 45.0%
ACAGCATGGTCCCAATAATG+AGG - chr4.4:20405055-20405074 None:intergenic 45.0%
ACATAGCGAGGATCAGGAAT+TGG + chr4.4:20403476-20403495 MS.gene35064:CDS 45.0%
ACCACTCAATATCCGGAATC+GGG - chr4.4:20404205-20404224 None:intergenic 45.0%
ACGACAGGAGATGATTAGAG+AGG + chr4.4:20404448-20404467 MS.gene35064:CDS 45.0%
AGCTCAAGCAAAAGAGCAGA+AGG + chr4.4:20404079-20404098 MS.gene35064:CDS 45.0%
ATACGATGGAGACGAAAGAG+AGG + chr4.4:20403371-20403390 MS.gene35064:CDS 45.0%
ATGCTGGGGTTCCTCATTAT+TGG + chr4.4:20405041-20405060 MS.gene35064:CDS 45.0%
CAAACAAGCACAGCTAGCAA+AGG + chr4.4:20404103-20404122 MS.gene35064:CDS 45.0%
CACCACTCAATATCCGGAAT+CGG - chr4.4:20404206-20404225 None:intergenic 45.0%
CATAGAACCGCCAAAACCAA+AGG + chr4.4:20404475-20404494 MS.gene35064:CDS 45.0%
CGACAGGAGATGATTAGAGA+GGG + chr4.4:20404449-20404468 MS.gene35064:CDS 45.0%
CTCATCTATTTCACGTCCCT+GGG - chr4.4:20403799-20403818 None:intergenic 45.0%
CTGAAGCAAAGAAGGAACGT+AGG + chr4.4:20403523-20403542 MS.gene35064:CDS 45.0%
CTTTGCTAGCTGTGCTTGTT+TGG - chr4.4:20404105-20404124 None:intergenic 45.0%
GATTCCGGATATTGAGTGGT+GGG + chr4.4:20404205-20404224 MS.gene35064:CDS 45.0%
GCCAACGGTACTATAGATGA+AGG + chr4.4:20404269-20404288 MS.gene35064:CDS 45.0%
GCCTTCATCTATAGTACCGT+TGG - chr4.4:20404273-20404292 None:intergenic 45.0%
GGACGTGAAATAGATGAGCA+TGG + chr4.4:20403801-20403820 MS.gene35064:CDS 45.0%
TACGATGGAGACGAAAGAGA+GGG + chr4.4:20403372-20403391 MS.gene35064:CDS 45.0%
TAGAGAACATAGCGAGGATC+AGG + chr4.4:20403470-20403489 MS.gene35064:CDS 45.0%
TCAGGATCGACATCCAATAC+AGG - chr4.4:20403918-20403937 None:intergenic 45.0%
TGCCTTAGCAAAGGCTAAGA+AGG + chr4.4:20403584-20403603 MS.gene35064:CDS 45.0%
TGCTGGGGTTCCTCATTATT+GGG + chr4.4:20405042-20405061 MS.gene35064:CDS 45.0%
! ACTAAGGACCCAAAAGCGTA+TGG + chr4.4:20404412-20404431 MS.gene35064:CDS 45.0%
! AGCCTTCTTAGCCTTTGCTA+AGG - chr4.4:20403589-20403608 None:intergenic 45.0%
! CTAAGGCATCACTACTAGAG+AGG - chr4.4:20403572-20403591 None:intergenic 45.0%
! CTCCTTAGCCATACGCTTTT+GGG - chr4.4:20404423-20404442 None:intergenic 45.0%
! TCTCCTTAGCCATACGCTTT+TGG - chr4.4:20404424-20404443 None:intergenic 45.0%
! TTTCTGAGGGACAGTAACGT+CGG - chr4.4:20403745-20403764 None:intergenic 45.0%
!! ACGTACTTCCTTTTCAAGCC+GGG - chr4.4:20404555-20404574 None:intergenic 45.0%
AAGACGAAGAACAGGAGCCT+TGG - chr4.4:20403772-20403791 None:intergenic 50.0%
AAGCAACTCAAGATCCCACC+CGG + chr4.4:20404534-20404553 MS.gene35064:CDS 50.0%
AATCGAAGCGCGATGAAGAG+AGG + chr4.4:20403412-20403431 MS.gene35064:CDS 50.0%
AATTCATCCTCTTAGGCCGC+AGG - chr4.4:20403989-20404008 None:intergenic 50.0%
ACCCGATTCCGGATATTGAG+TGG + chr4.4:20404201-20404220 MS.gene35064:CDS 50.0%
ACGTCCCACCACTCAATATC+CGG - chr4.4:20404212-20404231 None:intergenic 50.0%
AGCACAGCTAGCAAAGGCAA+AGG + chr4.4:20404109-20404128 MS.gene35064:CDS 50.0%
AGCTGCGTGAGAAGAAGGAA+AGG + chr4.4:20404642-20404661 MS.gene35064:CDS 50.0%
AGTGTTGTCGTGGTTGAAGG+TGG + chr4.4:20404812-20404831 MS.gene35064:CDS 50.0%
ATAAACTGTAGAGCGGGCCT+TGG - chr4.4:20404738-20404757 None:intergenic 50.0%
ATCGAAGCGCGATGAAGAGA+GGG + chr4.4:20403413-20403432 MS.gene35064:CDS 50.0%
CGATTCCGGATATTGAGTGG+TGG + chr4.4:20404204-20404223 MS.gene35064:CDS 50.0%
CTCGATACTCCTCGCATAAG+AGG + chr4.4:20403442-20403461 MS.gene35064:CDS 50.0%
GACGAAAGAGAGGGTAGCAA+AGG + chr4.4:20403381-20403400 MS.gene35064:CDS 50.0%
GCTCATCTATTTCACGTCCC+TGG - chr4.4:20403800-20403819 None:intergenic 50.0%
GTGAGCAGGCTCACATAGAT+AGG + chr4.4:20404591-20404610 MS.gene35064:CDS 50.0%
TAAACTGTAGAGCGGGCCTT+GGG - chr4.4:20404737-20404756 None:intergenic 50.0%
TAACGTCGGGTGTAGCAATC+TGG - chr4.4:20403731-20403750 None:intergenic 50.0%
TATGGTGACATTGCCGCCAA+CGG + chr4.4:20404254-20404273 MS.gene35064:CDS 50.0%
TGTCCCTCAGAAACCAACCA+AGG + chr4.4:20403752-20403771 MS.gene35064:CDS 50.0%
! GACCCAAAAGCGTATGGCTA+AGG + chr4.4:20404418-20404437 MS.gene35064:CDS 50.0%
! GGATGTGCGGTGATTTGTGA+TGG + chr4.4:20404785-20404804 MS.gene35064:CDS 50.0%
! TTCTGAGGGACAGTAACGTC+GGG - chr4.4:20403744-20403763 None:intergenic 50.0%
! TTGATAGGTTGAGGTGGTGG+AGG - chr4.4:20404368-20404387 None:intergenic 50.0%
!! ACTTCCTTTTCAAGCCGGGT+GGG - chr4.4:20404551-20404570 None:intergenic 50.0%
!! GAGCCTTGGTTGGTTTCTGA+GGG - chr4.4:20403758-20403777 None:intergenic 50.0%
!! GTTGGCTTGATAGGTTGAGG+TGG - chr4.4:20404374-20404393 None:intergenic 50.0%
!! TACTTCCTTTTCAAGCCGGG+TGG - chr4.4:20404552-20404571 None:intergenic 50.0%
!! TGATAGGTTGAGGTGGTGGA+GGG - chr4.4:20404367-20404386 None:intergenic 50.0%
CGAAGAACAGGAGCCTTGGT+TGG - chr4.4:20403768-20403787 None:intergenic 55.0%
GACGTGGCACTTCTGCATTC+TGG + chr4.4:20404227-20404246 MS.gene35064:CDS 55.0%
GTTCTTCGTCTTGACGCCCA+GGG + chr4.4:20403780-20403799 MS.gene35064:CDS 55.0%
TGCTGAGCTGCGTGAGAAGA+AGG + chr4.4:20404637-20404656 MS.gene35064:CDS 55.0%
TGGGCGTCAAGACGAAGAAC+AGG - chr4.4:20403780-20403799 None:intergenic 55.0%
TGTTCTTCGTCTTGACGCCC+AGG + chr4.4:20403779-20403798 MS.gene35064:CDS 55.0%
TTCTTCTCACGCAGCTCAGC+AGG - chr4.4:20404638-20404657 None:intergenic 55.0%
! AGATCCCACCCGGCTTGAAA+AGG + chr4.4:20404544-20404563 MS.gene35064:CDS 55.0%
! GGATATTGAGTGGTGGGACG+TGG + chr4.4:20404211-20404230 MS.gene35064:CDS 55.0%
! GGCTAAGGAGAAAGCACGAC+AGG + chr4.4:20404433-20404452 MS.gene35064:CDS 55.0%
! GGCTTGATAGGTTGAGGTGG+TGG - chr4.4:20404371-20404390 None:intergenic 55.0%
!! GATAGGTTGAGGTGGTGGAG+GGG - chr4.4:20404366-20404385 None:intergenic 55.0%
!! GGAGCCTTGGTTGGTTTCTG+AGG - chr4.4:20403759-20403778 None:intergenic 55.0%
CAAAGCTCCTGCGGCCTAAG+AGG + chr4.4:20403979-20403998 MS.gene35064:CDS 60.0%
GAAGCGCGATGAAGAGAGGG+AGG + chr4.4:20403416-20403435 MS.gene35064:CDS 60.0%
GGCTCAGCAGGCGGTTCAAT+AGG - chr4.4:20404341-20404360 None:intergenic 60.0%
TCGGGTGTAGCAATCTGGCC+AGG - chr4.4:20403726-20403745 None:intergenic 60.0%
GCAGGCGGTTCAATAGGCCG+AGG - chr4.4:20404335-20404354 None:intergenic 65.0%
TGCAGCTGCTGAGCGTGAGC+AGG + chr4.4:20404577-20404596 MS.gene35064:CDS 65.0%
!! AGGTTGAGGTGGTGGAGGGG+CGG - chr4.4:20404363-20404382 None:intergenic 65.0%
!! GGTTGAGGTGGTGGAGGGGC+GGG - chr4.4:20404362-20404381 None:intergenic 70.0%
!! GGAGGGGCGGGCTCAGCAGG+CGG - chr4.4:20404350-20404369 None:intergenic 80.0%
!! GGTGGAGGGGCGGGCTCAGC+AGG - chr4.4:20404353-20404372 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 20403366 20405102 20403366 ID=MS.gene35064
chr4.4 mRNA 20403366 20405102 20403366 ID=MS.gene35064.t1;Parent=MS.gene35064
chr4.4 exon 20403366 20405102 20403366 ID=MS.gene35064.t1.exon1;Parent=MS.gene35064.t1
chr4.4 CDS 20403366 20405102 20403366 ID=cds.MS.gene35064.t1;Parent=MS.gene35064.t1
Gene Sequence

>MS.gene35064

ATGGGATACGATGGAGACGAAAGAGAGGGTAGCAAAGGTAGGTCTAAATCGAAGCGCGATGAAGAGAGGGAGGATTCTCGATACTCCTCGCATAAGAGGAAAGATAGAGAACATAGCGAGGATCAGGAATTGGATGATGATAAGAGAATTAGGGTTTCTGAAGCAAAGAAGGAACGTAGGAGATTTGGTGATAAACTTGGAAGCCTCTCTAGTAGTGATGCCTTAGCAAAGGCTAAGAAGGCTTTACAAATGCAGAAATTGTTGTCAGAGAAGCTGAAGAAAATTCCTCAGTTGAATAACTCAAATTCAGAAGAAAGCACACAGTTGAGTAACAAGACTGAATCTACAGCTATGTTTCCTGGCCAGATTGCTACACCCGACGTTACTGTCCCTCAGAAACCAACCAAGGCTCCTGTTCTTCGTCTTGACGCCCAGGGACGTGAAATAGATGAGCATGGAAATGTTGTTAATGTAACTAAGCCAAGCAACCTTAGCACATTAAAGGTCAATATTAATAAACACAAGAGAGATGCATTTGAAATACTAAAACCTGTATTGGATGTCGATCCTGAATCTAATCCTCATTTTGATGAGAGAATGGGTATCAACAAAACAAAGCTCCTGCGGCCTAAGAGGATGAATTTTCTCTTTGTGGAGGAAGGAAAATGGTCAAGAGACGCTGAATCAATAAAATTGAAGAGTAAATTTGGAGAAGCTCAAGCAAAAGAGCAGAAGGCCAAACAAGCACAGCTAGCAAAGGCAAAGGCTTCTCCTGATATCAATCCCAACTTAATAGAAATTACAGAGAGAGTTGTAATGAAAGCAAAACTGAAGGACCCGATTCCGGATATTGAGTGGTGGGACGTGGCACTTCTGCATTCTGGAAATTATGGTGACATTGCCGCCAACGGTACTATAGATGAAGGCAATATGAAAATGGAAGAATTCAACTTTCATGTGGAAATTCCTCGGCCTATTGAACCGCCTGCTGAGCCCGCCCCTCCACCACCTCAACCTATCAAGCCAACTAAACAGGAGCAGAAGAAACTAAGGACCCAAAAGCGTATGGCTAAGGAGAAAGCACGACAGGAGATGATTAGAGAGGGTGTCATAGAACCGCCAAAACCAAAGGTCAAGATGAGTAATTTACATAAGGTACTAGGGACTGAAGCAACTCAAGATCCCACCCGGCTTGAAAAGGAAGTACGTAATGCAGCTGCTGAGCGTGAGCAGGCTCACATAGATAGGAATATTGCACGCAAGCTTACTCCTGCTGAGCTGCGTGAGAAGAAGGAAAGGAAGTTGTTTCATGACCCAAATACATTGGACACACTTGTCTCACTTTACAGGGTAAATGACTTCTCTCATCCCAAGGCCCGCTCTACAGTTTATGTTAATGCTCAACAAAATCGTTTGACTGGATGTGCGGTGATTTGTGATGGTATTAGTGTTGTCGTGGTTGAAGGTGGAAGCAAGTCAATTAAGAGGTATGGGAAACTTATGCTTAGACGCATCAATTGGAATGATGTTTCAAAAGAGAAAGAAGATAATGAAGATTCAGACGATGCCAATAAATGTGTTCTGGTGTGGCAAGGAAGTGTTGCCAAATCGAGTTTCAATAGGTTCAGTGTTCATGACTGCATCACCGAAGCAGCTGCTCGTAAAGTTTTTGTGGATGCTGGGGTTCCTCATTATTGGGACCATGCTGTCAACTATACAAATGATGATGCTGTTTGA
Protein sequence

>MS.gene35064.t1

MGYDGDEREGSKGRSKSKRDEEREDSRYSSHKRKDREHSEDQELDDDKRIRVSEAKKERRRFGDKLGSLSSSDALAKAKKALQMQKLLSEKLKKIPQLNNSNSEESTQLSNKTESTAMFPGQIATPDVTVPQKPTKAPVLRLDAQGREIDEHGNVVNVTKPSNLSTLKVNINKHKRDAFEILKPVLDVDPESNPHFDERMGINKTKLLRPKRMNFLFVEEGKWSRDAESIKLKSKFGEAQAKEQKAKQAQLAKAKASPDINPNLIEITERVVMKAKLKDPIPDIEWWDVALLHSGNYGDIAANGTIDEGNMKMEEFNFHVEIPRPIEPPAEPAPPPPQPIKPTKQEQKKLRTQKRMAKEKARQEMIREGVIEPPKPKVKMSNLHKVLGTEATQDPTRLEKEVRNAAAEREQAHIDRNIARKLTPAELREKKERKLFHDPNTLDTLVSLYRVNDFSHPKARSTVYVNAQQNRLTGCAVICDGISVVVVEGGSKSIKRYGKLMLRRINWNDVSKEKEDNEDSDDANKCVLVWQGSVAKSSFNRFSVHDCITEAAARKVFVDAGVPHYWDHAVNYTNDDAV