AlfalfaGEDB | MS.gene69196

M. sativa cultivar XinJiangDaYe / MS.gene69196

KEGG: K07565
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005634 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0042255 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0070925 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03657.16 PF17833.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69196.t1	XP_013462141.1	99.5	187	1	0	1	187	1	187	2.30E-103	384.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69196.t1	A4QND5	61.5	187	64	1	1	187	1	179	2.0e-60	233.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69196.t1	I3ST48	99.5	187	1	0	1	187	1	187	1.6e-103	384.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050574	MS.gene69196	0.81409	1.84E-51	-1.69E-46
MS.gene056257	MS.gene69196	0.800375	1.48E-48	-1.69E-46
MS.gene056605	MS.gene69196	0.803627	3.19E-49	-1.69E-46
MS.gene058030	MS.gene69196	0.819489	1.13E-52	-1.69E-46
MS.gene060830	MS.gene69196	0.832214	1.07E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene09414	MS.gene69196	PPI
MS.gene007855	MS.gene69196	PPI
MS.gene05640	MS.gene69196	PPI
MS.gene69196	MS.gene044819	PPI
MS.gene92142	MS.gene69196	PPI
MS.gene64512	MS.gene69196	PPI
MS.gene81498	MS.gene69196	PPI
MS.gene032323	MS.gene69196	PPI
MS.gene000480	MS.gene69196	PPI
MS.gene69196	MS.gene041557	PPI
MS.gene90360	MS.gene69196	PPI
MS.gene52150	MS.gene69196	PPI
MS.gene04336	MS.gene69196	PPI
MS.gene044537	MS.gene69196	PPI
MS.gene058018	MS.gene69196	PPI
MS.gene70551	MS.gene69196	PPI
MS.gene061905	MS.gene69196	PPI
MS.gene08434	MS.gene69196	PPI
MS.gene53720	MS.gene69196	PPI
MS.gene020215	MS.gene69196	PPI
MS.gene84016	MS.gene69196	PPI
MS.gene09204	MS.gene69196	PPI
MS.gene04159	MS.gene69196	PPI
MS.gene81001	MS.gene69196	PPI
MS.gene066348	MS.gene69196	PPI
MS.gene045818	MS.gene69196	PPI
MS.gene04079	MS.gene69196	PPI
MS.gene37937	MS.gene69196	PPI
MS.gene09038	MS.gene69196	PPI
MS.gene070298	MS.gene69196	PPI
MS.gene039360	MS.gene69196	PPI
MS.gene073613	MS.gene69196	PPI
MS.gene51111	MS.gene69196	PPI
MS.gene067030	MS.gene69196	PPI
MS.gene69196	MS.gene75598	PPI
MS.gene032998	MS.gene69196	PPI
MS.gene80570	MS.gene69196	PPI
MS.gene98889	MS.gene69196	PPI
MS.gene059880	MS.gene69196	PPI
MS.gene94183	MS.gene69196	PPI
MS.gene88081	MS.gene69196	PPI
MS.gene035194	MS.gene69196	PPI
MS.gene037902	MS.gene69196	PPI
MS.gene77392	MS.gene69196	PPI
MS.gene69196	MS.gene057738	PPI
MS.gene69196	MS.gene066830	PPI
MS.gene43350	MS.gene69196	PPI
MS.gene86675	MS.gene69196	PPI
MS.gene08109	MS.gene69196	PPI
MS.gene80786	MS.gene69196	PPI
MS.gene035331	MS.gene69196	PPI
MS.gene69196	MS.gene75579	PPI
MS.gene79721	MS.gene69196	PPI
MS.gene061835	MS.gene69196	PPI
MS.gene30239	MS.gene69196	PPI
MS.gene045265	MS.gene69196	PPI
MS.gene038784	MS.gene69196	PPI
MS.gene81341	MS.gene69196	PPI
MS.gene035396	MS.gene69196	PPI
MS.gene09845	MS.gene69196	PPI
MS.gene05689	MS.gene69196	PPI
MS.gene32240	MS.gene69196	PPI
MS.gene036487	MS.gene69196	PPI
MS.gene018599	MS.gene69196	PPI
MS.gene022752	MS.gene69196	PPI
MS.gene047734	MS.gene69196	PPI
MS.gene066300	MS.gene69196	PPI
MS.gene034931	MS.gene69196	PPI
MS.gene39294	MS.gene69196	PPI
MS.gene001917	MS.gene69196	PPI
MS.gene060923	MS.gene69196	PPI
MS.gene23437	MS.gene69196	PPI
MS.gene003492	MS.gene69196	PPI
MS.gene018651	MS.gene69196	PPI
MS.gene069038	MS.gene69196	PPI
MS.gene38996	MS.gene69196	PPI
MS.gene45646	MS.gene69196	PPI
MS.gene041623	MS.gene69196	PPI
MS.gene010453	MS.gene69196	PPI
MS.gene059878	MS.gene69196	PPI
MS.gene95644	MS.gene69196	PPI
MS.gene040651	MS.gene69196	PPI
MS.gene068200	MS.gene69196	PPI
MS.gene69196	MS.gene98171	PPI
MS.gene52105	MS.gene69196	PPI
MS.gene045239	MS.gene69196	PPI
MS.gene066811	MS.gene69196	PPI
MS.gene59609	MS.gene69196	PPI
MS.gene90945	MS.gene69196	PPI
MS.gene018553	MS.gene69196	PPI
MS.gene59319	MS.gene69196	PPI
MS.gene031378	MS.gene69196	PPI
MS.gene035458	MS.gene69196	PPI
MS.gene04418	MS.gene69196	PPI
MS.gene99779	MS.gene69196	PPI
MS.gene003435	MS.gene69196	PPI
MS.gene69196	MS.gene96949	PPI
MS.gene47347	MS.gene69196	PPI
MS.gene003490	MS.gene69196	PPI
MS.gene29719	MS.gene69196	PPI
MS.gene066301	MS.gene69196	PPI
MS.gene78972	MS.gene69196	PPI
MS.gene41329	MS.gene69196	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69196.t1	MTR_3g112090	99.465	187	1	0	1	187	1	187	1.14e-139	387

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69196.t1	AT4G15770	83.422	187	31	0	1	187	1	187	3.76e-119	335

Find 45 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGATGTGCCTTTGGGTTT+TGG	0.195441	3.1:-89449073	MS.gene69196:CDS
TTCTATGTCGGATGTGCCTT+TGG	0.274575	3.1:-89449080	MS.gene69196:CDS
TGGTATTGTTGTGCTTCATC+AGG	0.357825	3.1:-89449005	MS.gene69196:CDS
GTGCTTCATCAGGGTGATAT+TGG	0.380581	3.1:-89448995	MS.gene69196:CDS
TCTATGTCGGATGTGCCTTT+GGG	0.403032	3.1:-89449079	MS.gene69196:CDS
CTAAGTCTACACAGGATTGT+AGG	0.417131	3.1:-89449042	MS.gene69196:CDS
GTGATATTGGAGAGTATTTG+AGG	0.418524	3.1:-89448982	MS.gene69196:CDS
GAGTGCGTTGGGTCGCATTA+CGG	0.440043	3.1:-89449137	MS.gene69196:CDS
CTCGTTTCTGTCTCGTCCAA+AGG	0.450814	3.1:+89449499	None:intergenic
TGGAGAGTATTTGAGGATTG+AGG	0.451876	3.1:-89448975	MS.gene69196:CDS
ATTACGGAGAATATTGTTGC+TGG	0.453018	3.1:-89449121	MS.gene69196:CDS
AGAATATTGTTGCTGGTGAT+GGG	0.475550	3.1:-89449114	MS.gene69196:CDS
GAGAATATTGTTGCTGGTGA+TGG	0.477429	3.1:-89449115	MS.gene69196:CDS
TGAAGCACAACAATACCATT+AGG	0.481187	3.1:+89449010	None:intergenic
GTTCCGAGGGAAACGAGGTT+AGG	0.488759	3.1:+89449301	None:intergenic
TACACAGGATTGTAGGAAGT+TGG	0.488772	3.1:-89449035	MS.gene69196:CDS
GTAGCGACCAGGATTTGAAT+CGG	0.501537	3.1:+89449399	None:intergenic
GTTTCCCTCGGAACGTGTAT+CGG	0.505430	3.1:-89449292	MS.gene69196:CDS
TGTAGGAAGTTGGATCCTAA+TGG	0.511689	3.1:-89449025	MS.gene69196:CDS
TTTAAGCCATACCTTATGCT+TGG	0.513488	3.1:+89449204	None:intergenic
TGCTGCGAATGCCAAGCATA+AGG	0.516412	3.1:-89449215	MS.gene69196:CDS
GAAGGTCCCGATTCAAATCC+TGG	0.525646	3.1:-89449406	MS.gene69196:CDS
GAATCGGGACCTTCGTGTGA+GGG	0.536023	3.1:+89449415	None:intergenic
AGACCTAACCTCGTTTCCCT+CGG	0.540105	3.1:-89449304	MS.gene69196:CDS
GAGGTTAGGTCTAGCGATGT+TGG	0.552615	3.1:+89449315	None:intergenic
CATGTGTTGAAGAGTGCGTT+GGG	0.555269	3.1:-89449148	MS.gene69196:CDS
TAGCGACCAGGATTTGAATC+GGG	0.568635	3.1:+89449400	None:intergenic
GAAAAGCTCTTCAAATTCGT+AGG	0.571649	3.1:-89449466	MS.gene69196:CDS
CGAATGCCAAGCATAAGGTA+TGG	0.574051	3.1:-89449210	MS.gene69196:CDS
TCATGTGTTGAAGAGTGCGT+TGG	0.587350	3.1:-89449149	MS.gene69196:CDS
AGACGGAAACAGTAGCGACC+AGG	0.591481	3.1:+89449388	None:intergenic
GGTATTGTTGTGCTTCATCA+GGG	0.595232	3.1:-89449004	MS.gene69196:CDS
TGTTGGTGGCACGCTTAACG+AGG	0.595506	3.1:+89449332	None:intergenic
GTTAGGTCTAGCGATGTTGG+TGG	0.599334	3.1:+89449318	None:intergenic
TACACGTTCCGAGGGAAACG+AGG	0.615959	3.1:+89449296	None:intergenic
TGAATCGGGACCTTCGTGTG+AGG	0.619897	3.1:+89449414	None:intergenic
AAGTAAATTCAACGCCTGAA+CGG	0.628881	3.1:+89449237	None:intergenic
GAATATTGTTGCTGGTGATG+GGG	0.643723	3.1:-89449113	MS.gene69196:CDS
GTTGGTGGCACGCTTAACGA+GGG	0.651920	3.1:+89449333	None:intergenic
GGTGGCTGCTAAGTCTACAC+AGG	0.655794	3.1:-89449050	MS.gene69196:CDS
GCAGCCACCCCAAAACCCAA+AGG	0.658414	3.1:+89449064	None:intergenic
GTTGAAAATCCCTCACACGA+AGG	0.698320	3.1:-89449424	MS.gene69196:CDS
GGACGAGACAGAAACGAGCG+TGG	0.721090	3.1:-89449494	MS.gene69196:CDS
TGTATCGGAAAATACACACA+CGG	0.738380	3.1:-89449277	MS.gene69196:CDS
ATCGGAAAATACACACACGG+TGG	0.745885	3.1:-89449274	MS.gene69196:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAGACATCTCAGATTG+TGG	+	chr3.1:89449291-89449310	None:intergenic	30.0%
!	AGATTTTGTTCTTGTTGAGA+CGG	+	chr3.1:89449101-89449120	None:intergenic	30.0%
!!!	CTGAGATGTCTTTTTTGTAT+GGG	-	chr3.1:89449295-89449314	MS.gene69196:CDS	30.0%
!!!	GGTTGTTGTTTTTTCTATGT+CGG	-	chr3.1:89449377-89449396	MS.gene69196:CDS	30.0%
!!!	TCTGAGATGTCTTTTTTGTA+TGG	-	chr3.1:89449294-89449313	MS.gene69196:CDS	30.0%
	AAGTAAATTCAACGCCTGAA+CGG	+	chr3.1:89449235-89449254	None:intergenic	35.0%
	AGAATATTGTTGCTGGTGAT+GGG	-	chr3.1:89449355-89449374	MS.gene69196:CDS	35.0%
	ATTACGGAGAATATTGTTGC+TGG	-	chr3.1:89449348-89449367	MS.gene69196:CDS	35.0%
	GAAAAGCTCTTCAAATTCGT+AGG	-	chr3.1:89449003-89449022	MS.gene69196:CDS	35.0%
	TGAAGCACAACAATACCATT+AGG	+	chr3.1:89449462-89449481	None:intergenic	35.0%
	TGTATCGGAAAATACACACA+CGG	-	chr3.1:89449192-89449211	MS.gene69196:CDS	35.0%
	TTTAAGCCATACCTTATGCT+TGG	+	chr3.1:89449268-89449287	None:intergenic	35.0%
!	AAATTTTCATCTCACCGTTC+AGG	-	chr3.1:89449218-89449237	MS.gene69196:CDS	35.0%
!!	GTGATATTGGAGAGTATTTG+AGG	-	chr3.1:89449487-89449506	MS.gene69196:CDS	35.0%
	CTAAGTCTACACAGGATTGT+AGG	-	chr3.1:89449427-89449446	MS.gene69196:CDS	40.0%
	GAGAATATTGTTGCTGGTGA+TGG	-	chr3.1:89449354-89449373	MS.gene69196:CDS	40.0%
	GGTATTGTTGTGCTTCATCA+GGG	-	chr3.1:89449465-89449484	MS.gene69196:CDS	40.0%
	TACACAGGATTGTAGGAAGT+TGG	-	chr3.1:89449434-89449453	MS.gene69196:CDS	40.0%
	TGGTATTGTTGTGCTTCATC+AGG	-	chr3.1:89449464-89449483	MS.gene69196:CDS	40.0%
	TGTAGGAAGTTGGATCCTAA+TGG	-	chr3.1:89449444-89449463	MS.gene69196:CDS	40.0%
!	GAATATTGTTGCTGGTGATG+GGG	-	chr3.1:89449356-89449375	MS.gene69196:CDS	40.0%
!!	TGGAGAGTATTTGAGGATTG+AGG	-	chr3.1:89449494-89449513	MS.gene69196:CDS	40.0%
	ATCGGAAAATACACACACGG+TGG	-	chr3.1:89449195-89449214	MS.gene69196:CDS	45.0%
	CGAATGCCAAGCATAAGGTA+TGG	-	chr3.1:89449259-89449278	MS.gene69196:CDS	45.0%
	GTAGCGACCAGGATTTGAAT+CGG	+	chr3.1:89449073-89449092	None:intergenic	45.0%
	GTGCTTCATCAGGGTGATAT+TGG	-	chr3.1:89449474-89449493	MS.gene69196:CDS	45.0%
	GTTGAAAATCCCTCACACGA+AGG	-	chr3.1:89449045-89449064	MS.gene69196:CDS	45.0%
	TAGCGACCAGGATTTGAATC+GGG	+	chr3.1:89449072-89449091	None:intergenic	45.0%
	TCTATGTCGGATGTGCCTTT+GGG	-	chr3.1:89449390-89449409	MS.gene69196:CDS	45.0%
	TTCTATGTCGGATGTGCCTT+TGG	-	chr3.1:89449389-89449408	MS.gene69196:CDS	45.0%
!	ATTTTCCGATACACGTTCCG+AGG	+	chr3.1:89449185-89449204	None:intergenic	45.0%
!	CATGTGTTGAAGAGTGCGTT+GGG	-	chr3.1:89449321-89449340	MS.gene69196:CDS	45.0%
!	TCATGTGTTGAAGAGTGCGT+TGG	-	chr3.1:89449320-89449339	MS.gene69196:CDS	45.0%
!	TTTTCCGATACACGTTCCGA+GGG	+	chr3.1:89449184-89449203	None:intergenic	45.0%
	AGACCTAACCTCGTTTCCCT+CGG	-	chr3.1:89449165-89449184	MS.gene69196:CDS	50.0%
	CTCGTTTCTGTCTCGTCCAA+AGG	+	chr3.1:89448973-89448992	None:intergenic	50.0%
	GAAGGTCCCGATTCAAATCC+TGG	-	chr3.1:89449063-89449082	MS.gene69196:CDS	50.0%
	GAGGTTAGGTCTAGCGATGT+TGG	+	chr3.1:89449157-89449176	None:intergenic	50.0%
	GTTAGGTCTAGCGATGTTGG+TGG	+	chr3.1:89449154-89449173	None:intergenic	50.0%
	GTTTCCCTCGGAACGTGTAT+CGG	-	chr3.1:89449177-89449196	MS.gene69196:CDS	50.0%
	TGCTGCGAATGCCAAGCATA+AGG	-	chr3.1:89449254-89449273	MS.gene69196:CDS	50.0%
!	CGTGTGAGGGATTTTCAACG+AGG	+	chr3.1:89449044-89449063	None:intergenic	50.0%
!!	CGGATGTGCCTTTGGGTTTT+GGG	-	chr3.1:89449397-89449416	MS.gene69196:CDS	50.0%
!!	GGATGTGCCTTTGGGTTTTG+GGG	-	chr3.1:89449398-89449417	MS.gene69196:CDS	50.0%
!!	TCGGATGTGCCTTTGGGTTT+TGG	-	chr3.1:89449396-89449415	MS.gene69196:CDS	50.0%
	AGACGGAAACAGTAGCGACC+AGG	+	chr3.1:89449084-89449103	None:intergenic	55.0%
	GAATCGGGACCTTCGTGTGA+GGG	+	chr3.1:89449057-89449076	None:intergenic	55.0%
	GAGTGCGTTGGGTCGCATTA+CGG	-	chr3.1:89449332-89449351	MS.gene69196:CDS	55.0%
	GGTGGCTGCTAAGTCTACAC+AGG	-	chr3.1:89449419-89449438	MS.gene69196:CDS	55.0%
	GTTCCGAGGGAAACGAGGTT+AGG	+	chr3.1:89449171-89449190	None:intergenic	55.0%
	TACACGTTCCGAGGGAAACG+AGG	+	chr3.1:89449176-89449195	None:intergenic	55.0%
	TGAATCGGGACCTTCGTGTG+AGG	+	chr3.1:89449058-89449077	None:intergenic	55.0%
!!	GTTGGTGGCACGCTTAACGA+GGG	+	chr3.1:89449139-89449158	None:intergenic	55.0%
!!	TGTTGGTGGCACGCTTAACG+AGG	+	chr3.1:89449140-89449159	None:intergenic	55.0%
!!!	TGTGCCTTTGGGTTTTGGGG+TGG	-	chr3.1:89449401-89449420	MS.gene69196:CDS	55.0%
	GCAGCCACCCCAAAACCCAA+AGG	+	chr3.1:89449408-89449427	None:intergenic	60.0%
	GGACGAGACAGAAACGAGCG+TGG	-	chr3.1:89448975-89448994	MS.gene69196:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	89448964	89449527	89448964	ID=MS.gene69196
chr3.1	mRNA	89448964	89449527	89448964	ID=MS.gene69196.t1;Parent=MS.gene69196
chr3.1	exon	89448964	89449527	89448964	ID=MS.gene69196.t1.exon1;Parent=MS.gene69196.t1
chr3.1	CDS	89448964	89449527	89448964	ID=cds.MS.gene69196.t1;Parent=MS.gene69196.t1

Gene Sequence

>MS.gene69196

ATGAGACCTTTGGACGAGACAGAAACGAGCGTGGTATTCGAAAAGCTCTTCAAATTCGTAGGAAACAATCTCAAAAACCTCGTTGAAAATCCCTCACACGAAGGTCCCGATTCAAATCCTGGTCGCTACTGTTTCCGTCTCAACAAGAACAAAATCTACTACTGCAGTGAATCCCTCGTTAAGCGTGCCACCAACATCGCTAGACCTAACCTCGTTTCCCTCGGAACGTGTATCGGAAAATACACACACGGTGGAAATTTTCATCTCACCGTTCAGGCGTTGAATTTACTTGCTGCGAATGCCAAGCATAAGGTATGGCTTAAACCACAATCTGAGATGTCTTTTTTGTATGGGAATCATGTGTTGAAGAGTGCGTTGGGTCGCATTACGGAGAATATTGTTGCTGGTGATGGGGTTGTTGTTTTTTCTATGTCGGATGTGCCTTTGGGTTTTGGGGTGGCTGCTAAGTCTACACAGGATTGTAGGAAGTTGGATCCTAATGGTATTGTTGTGCTTCATCAGGGTGATATTGGAGAGTATTTGAGGATTGAGGATGAGCTTTGA

Protein sequence

>MS.gene69196.t1

MRPLDETETSVVFEKLFKFVGNNLKNLVENPSHEGPDSNPGRYCFRLNKNKIYYCSESLVKRATNIARPNLVSLGTCIGKYTHGGNFHLTVQALNLLAANAKHKVWLKPQSEMSFLYGNHVLKSALGRITENIVAGDGVVVFSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQGDIGEYLRIEDEL