AlfalfaGEDB | MS.gene42324

M. sativa cultivar XinJiangDaYe / MS.gene42324

KEGG: K07565
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005634 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0042255 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0070925 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03657.16 PF17833.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42324.t1	XP_013462141.1	99.5	187	1	0	1	187	1	187	2.30E-103	384.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42324.t1	A4QND5	61.5	187	64	1	1	187	1	179	2.0e-60	233.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42324.t1	I3ST48	99.5	187	1	0	1	187	1	187	1.6e-103	384.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050574	MS.gene42324	0.820218	7.70E-53	-1.69E-46
MS.gene053007	MS.gene42324	0.851314	9.62E-61	-1.69E-46
MS.gene056257	MS.gene42324	0.845445	4.04E-59	-1.69E-46
MS.gene058030	MS.gene42324	0.860595	1.85E-63	-1.69E-46
MS.gene060830	MS.gene42324	0.825002	5.94E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene42324	MS.gene64512	PPI
MS.gene42324	MS.gene034931	PPI
MS.gene039360	MS.gene42324	PPI
MS.gene073613	MS.gene42324	PPI
MS.gene067030	MS.gene42324	PPI
MS.gene032998	MS.gene42324	PPI
MS.gene80570	MS.gene42324	PPI
MS.gene047734	MS.gene42324	PPI
MS.gene059880	MS.gene42324	PPI
MS.gene003492	MS.gene42324	PPI
MS.gene069038	MS.gene42324	PPI
MS.gene88081	MS.gene42324	PPI
MS.gene035194	MS.gene42324	PPI
MS.gene38996	MS.gene42324	PPI
MS.gene037902	MS.gene42324	PPI
MS.gene010453	MS.gene42324	PPI
MS.gene43350	MS.gene42324	PPI
MS.gene86675	MS.gene42324	PPI
MS.gene08109	MS.gene42324	PPI
MS.gene42324	MS.gene038784	PPI
MS.gene80786	MS.gene42324	PPI
MS.gene95644	MS.gene42324	PPI
MS.gene42324	MS.gene044819	PPI
MS.gene045265	MS.gene42324	PPI
MS.gene068200	MS.gene42324	PPI
MS.gene42324	MS.gene75598	PPI
MS.gene09845	MS.gene42324	PPI
MS.gene42324	MS.gene041557	PPI
MS.gene32240	MS.gene42324	PPI
MS.gene036487	MS.gene42324	PPI
MS.gene045239	MS.gene42324	PPI
MS.gene018599	MS.gene42324	PPI
MS.gene018553	MS.gene42324	PPI
MS.gene59319	MS.gene42324	PPI
MS.gene42324	MS.gene90945	PPI
MS.gene99779	MS.gene42324	PPI
MS.gene42324	MS.gene057738	PPI
MS.gene42324	MS.gene066830	PPI
MS.gene42324	MS.gene060923	PPI
MS.gene39294	MS.gene42324	PPI
MS.gene23437	MS.gene42324	PPI
MS.gene94183	MS.gene42324	PPI
MS.gene42324	MS.gene018651	PPI
MS.gene45646	MS.gene42324	PPI
MS.gene003490	MS.gene42324	PPI
MS.gene42324	MS.gene75579	PPI
MS.gene41329	MS.gene42324	PPI
MS.gene059878	MS.gene42324	PPI
MS.gene42324	MS.gene035331	PPI
MS.gene09414	MS.gene42324	PPI
MS.gene040651	MS.gene42324	PPI
MS.gene42324	MS.gene09204	PPI
MS.gene52105	MS.gene42324	PPI
MS.gene42324	MS.gene066300	PPI
MS.gene066811	MS.gene42324	PPI
MS.gene42324	MS.gene29719	PPI
MS.gene42324	MS.gene041623	PPI
MS.gene031378	MS.gene42324	PPI
MS.gene04418	MS.gene42324	PPI
MS.gene058018	MS.gene42324	PPI
MS.gene003435	MS.gene42324	PPI
MS.gene47347	MS.gene42324	PPI
MS.gene08434	MS.gene42324	PPI
MS.gene53720	MS.gene42324	PPI
MS.gene42324	MS.gene000480	PPI
MS.gene42324	MS.gene79721	PPI
MS.gene42324	MS.gene035458	PPI
MS.gene42324	MS.gene90360	PPI
MS.gene78972	MS.gene42324	PPI
MS.gene84016	MS.gene42324	PPI
MS.gene04159	MS.gene42324	PPI
MS.gene066348	MS.gene42324	PPI
MS.gene09038	MS.gene42324	PPI
MS.gene42324	MS.gene98171	PPI
MS.gene007855	MS.gene42324	PPI
MS.gene05640	MS.gene42324	PPI
MS.gene92142	MS.gene42324	PPI
MS.gene51111	MS.gene42324	PPI
MS.gene81498	MS.gene42324	PPI
MS.gene032323	MS.gene42324	PPI
MS.gene42324	MS.gene77392	PPI
MS.gene42324	MS.gene045818	PPI
MS.gene42324	MS.gene59609	PPI
MS.gene42324	MS.gene96949	PPI
MS.gene98889	MS.gene42324	PPI
MS.gene52150	MS.gene42324	PPI
MS.gene04336	MS.gene42324	PPI
MS.gene044537	MS.gene42324	PPI
MS.gene81341	MS.gene42324	PPI
MS.gene70551	MS.gene42324	PPI
MS.gene061905	MS.gene42324	PPI
MS.gene42324	MS.gene001917	PPI
MS.gene020215	MS.gene42324	PPI
MS.gene42324	MS.gene066301	PPI
MS.gene061835	MS.gene42324	PPI
MS.gene30239	MS.gene42324	PPI
MS.gene42324	MS.gene022752	PPI
MS.gene81001	MS.gene42324	PPI
MS.gene035396	MS.gene42324	PPI
MS.gene04079	MS.gene42324	PPI
MS.gene37937	MS.gene42324	PPI
MS.gene05689	MS.gene42324	PPI
MS.gene070298	MS.gene42324	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42324.t1	MTR_3g112090	99.465	187	1	0	1	187	1	187	1.14e-139	387

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene42324.t1	AT4G15770	83.422	187	31	0	1	187	1	187	3.76e-119	335

Find 45 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGATGTGCCTTTGGGTTT+TGG	0.195441	3.2:+89404693	MS.gene42324:CDS
TTCTATGTCGGATGTGCCTT+TGG	0.274575	3.2:+89404686	MS.gene42324:CDS
TGGTATTGTTGTGCTTCATC+AGG	0.357825	3.2:+89404761	MS.gene42324:CDS
GTGCTTCATCAGGGTGATAT+TGG	0.380581	3.2:+89404771	MS.gene42324:CDS
TCTATGTCGGATGTGCCTTT+GGG	0.403032	3.2:+89404687	MS.gene42324:CDS
CTAAGTCTACACAGGATTGT+AGG	0.417131	3.2:+89404724	MS.gene42324:CDS
GTGATATTGGAGAGTATTTG+AGG	0.418524	3.2:+89404784	MS.gene42324:CDS
GAGTGCGTTGGGTCGCATTA+CGG	0.440043	3.2:+89404629	MS.gene42324:CDS
CTCGTTTCTGTCTCGTCCAA+AGG	0.450814	3.2:-89404267	None:intergenic
TGGAGAGTATTTGAGGATTG+AGG	0.451876	3.2:+89404791	MS.gene42324:CDS
ATTACGGAGAATATTGTTGC+TGG	0.453018	3.2:+89404645	MS.gene42324:CDS
AGAATATTGTTGCTGGTGAT+GGG	0.475550	3.2:+89404652	MS.gene42324:CDS
GAGAATATTGTTGCTGGTGA+TGG	0.477429	3.2:+89404651	MS.gene42324:CDS
TGAAGCACAACAATACCATT+AGG	0.481187	3.2:-89404756	None:intergenic
GTTCCGAGGGAAACGAGGTT+AGG	0.488759	3.2:-89404465	None:intergenic
TACACAGGATTGTAGGAAGT+TGG	0.488772	3.2:+89404731	MS.gene42324:CDS
GTAGCGACCAGGATTTGAAT+CGG	0.501537	3.2:-89404367	None:intergenic
GTTTCCCTCGGAACGTGTAT+CGG	0.505430	3.2:+89404474	MS.gene42324:CDS
TGTAGGAAGTTGGATCCTAA+TGG	0.511689	3.2:+89404741	MS.gene42324:CDS
TTTAAGCCATACCTTATGCT+TGG	0.513488	3.2:-89404562	None:intergenic
TGCTGCGAATGCCAAGCATA+AGG	0.516412	3.2:+89404551	MS.gene42324:CDS
GAAGGTCCCGATTCAAATCC+TGG	0.525646	3.2:+89404360	MS.gene42324:CDS
GAATCGGGACCTTCGTGTGA+GGG	0.536023	3.2:-89404351	None:intergenic
AGACCTAACCTCGTTTCCCT+CGG	0.540105	3.2:+89404462	MS.gene42324:CDS
GAGGTTAGGTCTAGCGATGT+TGG	0.552615	3.2:-89404451	None:intergenic
CATGTGTTGAAGAGTGCGTT+GGG	0.555269	3.2:+89404618	MS.gene42324:CDS
TAGCGACCAGGATTTGAATC+GGG	0.568635	3.2:-89404366	None:intergenic
GAAAAGCTCTTCAAATTCGT+AGG	0.571649	3.2:+89404300	MS.gene42324:CDS
CGAATGCCAAGCATAAGGTA+TGG	0.574051	3.2:+89404556	MS.gene42324:CDS
TCATGTGTTGAAGAGTGCGT+TGG	0.587350	3.2:+89404617	MS.gene42324:CDS
AGACGGAAACAGTAGCGACC+AGG	0.591481	3.2:-89404378	None:intergenic
GGTATTGTTGTGCTTCATCA+GGG	0.595232	3.2:+89404762	MS.gene42324:CDS
TGTTGGTGGCACGCTTAACG+AGG	0.595506	3.2:-89404434	None:intergenic
GTTAGGTCTAGCGATGTTGG+TGG	0.599334	3.2:-89404448	None:intergenic
TACACGTTCCGAGGGAAACG+AGG	0.615959	3.2:-89404470	None:intergenic
TGAATCGGGACCTTCGTGTG+AGG	0.619897	3.2:-89404352	None:intergenic
AAGTAAATTCAACGCCTGAA+CGG	0.628881	3.2:-89404529	None:intergenic
GAATATTGTTGCTGGTGATG+GGG	0.643723	3.2:+89404653	MS.gene42324:CDS
GTTGGTGGCACGCTTAACGA+GGG	0.651920	3.2:-89404433	None:intergenic
GGTGGCTGCTAAGTCTACAC+AGG	0.655794	3.2:+89404716	MS.gene42324:CDS
GCAGCCACCCCAAAACCCAA+AGG	0.658414	3.2:-89404702	None:intergenic
GTTGAAAATCCCTCACACGA+AGG	0.698320	3.2:+89404342	MS.gene42324:CDS
GGACGAGACAGAAACGAGCG+TGG	0.721090	3.2:+89404272	MS.gene42324:CDS
TGTATCGGAAAATACACACA+CGG	0.738380	3.2:+89404489	MS.gene42324:CDS
ATCGGAAAATACACACACGG+TGG	0.745885	3.2:+89404492	MS.gene42324:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAGACATCTCAGATTG+TGG	-	chr3.2:89404588-89404607	None:intergenic	30.0%
!	AGATTTTGTTCTTGTTGAGA+CGG	-	chr3.2:89404398-89404417	None:intergenic	30.0%
!!!	CTGAGATGTCTTTTTTGTAT+GGG	+	chr3.2:89404592-89404611	MS.gene42324:CDS	30.0%
!!!	GGTTGTTGTTTTTTCTATGT+CGG	+	chr3.2:89404674-89404693	MS.gene42324:CDS	30.0%
!!!	TCTGAGATGTCTTTTTTGTA+TGG	+	chr3.2:89404591-89404610	MS.gene42324:CDS	30.0%
	AAGTAAATTCAACGCCTGAA+CGG	-	chr3.2:89404532-89404551	None:intergenic	35.0%
	AGAATATTGTTGCTGGTGAT+GGG	+	chr3.2:89404652-89404671	MS.gene42324:CDS	35.0%
	ATTACGGAGAATATTGTTGC+TGG	+	chr3.2:89404645-89404664	MS.gene42324:CDS	35.0%
	GAAAAGCTCTTCAAATTCGT+AGG	+	chr3.2:89404300-89404319	MS.gene42324:CDS	35.0%
	TGAAGCACAACAATACCATT+AGG	-	chr3.2:89404759-89404778	None:intergenic	35.0%
	TGTATCGGAAAATACACACA+CGG	+	chr3.2:89404489-89404508	MS.gene42324:CDS	35.0%
	TTTAAGCCATACCTTATGCT+TGG	-	chr3.2:89404565-89404584	None:intergenic	35.0%
!	AAATTTTCATCTCACCGTTC+AGG	+	chr3.2:89404515-89404534	MS.gene42324:CDS	35.0%
!!	GTGATATTGGAGAGTATTTG+AGG	+	chr3.2:89404784-89404803	MS.gene42324:CDS	35.0%
	CTAAGTCTACACAGGATTGT+AGG	+	chr3.2:89404724-89404743	MS.gene42324:CDS	40.0%
	GAGAATATTGTTGCTGGTGA+TGG	+	chr3.2:89404651-89404670	MS.gene42324:CDS	40.0%
	GGTATTGTTGTGCTTCATCA+GGG	+	chr3.2:89404762-89404781	MS.gene42324:CDS	40.0%
	TACACAGGATTGTAGGAAGT+TGG	+	chr3.2:89404731-89404750	MS.gene42324:CDS	40.0%
	TGGTATTGTTGTGCTTCATC+AGG	+	chr3.2:89404761-89404780	MS.gene42324:CDS	40.0%
	TGTAGGAAGTTGGATCCTAA+TGG	+	chr3.2:89404741-89404760	MS.gene42324:CDS	40.0%
!	GAATATTGTTGCTGGTGATG+GGG	+	chr3.2:89404653-89404672	MS.gene42324:CDS	40.0%
!!	TGGAGAGTATTTGAGGATTG+AGG	+	chr3.2:89404791-89404810	MS.gene42324:CDS	40.0%
	ATCGGAAAATACACACACGG+TGG	+	chr3.2:89404492-89404511	MS.gene42324:CDS	45.0%
	CGAATGCCAAGCATAAGGTA+TGG	+	chr3.2:89404556-89404575	MS.gene42324:CDS	45.0%
	GTAGCGACCAGGATTTGAAT+CGG	-	chr3.2:89404370-89404389	None:intergenic	45.0%
	GTGCTTCATCAGGGTGATAT+TGG	+	chr3.2:89404771-89404790	MS.gene42324:CDS	45.0%
	GTTGAAAATCCCTCACACGA+AGG	+	chr3.2:89404342-89404361	MS.gene42324:CDS	45.0%
	TAGCGACCAGGATTTGAATC+GGG	-	chr3.2:89404369-89404388	None:intergenic	45.0%
	TCTATGTCGGATGTGCCTTT+GGG	+	chr3.2:89404687-89404706	MS.gene42324:CDS	45.0%
	TTCTATGTCGGATGTGCCTT+TGG	+	chr3.2:89404686-89404705	MS.gene42324:CDS	45.0%
!	ATTTTCCGATACACGTTCCG+AGG	-	chr3.2:89404482-89404501	None:intergenic	45.0%
!	CATGTGTTGAAGAGTGCGTT+GGG	+	chr3.2:89404618-89404637	MS.gene42324:CDS	45.0%
!	TCATGTGTTGAAGAGTGCGT+TGG	+	chr3.2:89404617-89404636	MS.gene42324:CDS	45.0%
!	TTTTCCGATACACGTTCCGA+GGG	-	chr3.2:89404481-89404500	None:intergenic	45.0%
	AGACCTAACCTCGTTTCCCT+CGG	+	chr3.2:89404462-89404481	MS.gene42324:CDS	50.0%
	CTCGTTTCTGTCTCGTCCAA+AGG	-	chr3.2:89404270-89404289	None:intergenic	50.0%
	GAAGGTCCCGATTCAAATCC+TGG	+	chr3.2:89404360-89404379	MS.gene42324:CDS	50.0%
	GAGGTTAGGTCTAGCGATGT+TGG	-	chr3.2:89404454-89404473	None:intergenic	50.0%
	GTTAGGTCTAGCGATGTTGG+TGG	-	chr3.2:89404451-89404470	None:intergenic	50.0%
	GTTTCCCTCGGAACGTGTAT+CGG	+	chr3.2:89404474-89404493	MS.gene42324:CDS	50.0%
	TGCTGCGAATGCCAAGCATA+AGG	+	chr3.2:89404551-89404570	MS.gene42324:CDS	50.0%
!	CGTGTGAGGGATTTTCAACG+AGG	-	chr3.2:89404341-89404360	None:intergenic	50.0%
!!	CGGATGTGCCTTTGGGTTTT+GGG	+	chr3.2:89404694-89404713	MS.gene42324:CDS	50.0%
!!	GGATGTGCCTTTGGGTTTTG+GGG	+	chr3.2:89404695-89404714	MS.gene42324:CDS	50.0%
!!	TCGGATGTGCCTTTGGGTTT+TGG	+	chr3.2:89404693-89404712	MS.gene42324:CDS	50.0%
	AGACGGAAACAGTAGCGACC+AGG	-	chr3.2:89404381-89404400	None:intergenic	55.0%
	GAATCGGGACCTTCGTGTGA+GGG	-	chr3.2:89404354-89404373	None:intergenic	55.0%
	GAGTGCGTTGGGTCGCATTA+CGG	+	chr3.2:89404629-89404648	MS.gene42324:CDS	55.0%
	GGTGGCTGCTAAGTCTACAC+AGG	+	chr3.2:89404716-89404735	MS.gene42324:CDS	55.0%
	GTTCCGAGGGAAACGAGGTT+AGG	-	chr3.2:89404468-89404487	None:intergenic	55.0%
	TACACGTTCCGAGGGAAACG+AGG	-	chr3.2:89404473-89404492	None:intergenic	55.0%
	TGAATCGGGACCTTCGTGTG+AGG	-	chr3.2:89404355-89404374	None:intergenic	55.0%
!!	GTTGGTGGCACGCTTAACGA+GGG	-	chr3.2:89404436-89404455	None:intergenic	55.0%
!!	TGTTGGTGGCACGCTTAACG+AGG	-	chr3.2:89404437-89404456	None:intergenic	55.0%
!!!	TGTGCCTTTGGGTTTTGGGG+TGG	+	chr3.2:89404698-89404717	MS.gene42324:CDS	55.0%
	GCAGCCACCCCAAAACCCAA+AGG	-	chr3.2:89404705-89404724	None:intergenic	60.0%
	GGACGAGACAGAAACGAGCG+TGG	+	chr3.2:89404272-89404291	MS.gene42324:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	89404261	89404824	89404261	ID=MS.gene42324
chr3.2	mRNA	89404261	89404824	89404261	ID=MS.gene42324.t1;Parent=MS.gene42324
chr3.2	exon	89404261	89404824	89404261	ID=MS.gene42324.t1.exon1;Parent=MS.gene42324.t1
chr3.2	CDS	89404261	89404824	89404261	ID=cds.MS.gene42324.t1;Parent=MS.gene42324.t1

Gene Sequence

>MS.gene42324

ATGAGACCTTTGGACGAGACAGAAACGAGCGTGGTATTCGAAAAGCTCTTCAAATTCGTAGGAAACAATCTCAAAAACCTCGTTGAAAATCCCTCACACGAAGGTCCCGATTCAAATCCTGGTCGCTACTGTTTCCGTCTCAACAAGAACAAAATCTACTACTGCAGTGAATCCCTCGTTAAGCGTGCCACCAACATCGCTAGACCTAACCTCGTTTCCCTCGGAACGTGTATCGGAAAATACACACACGGTGGAAATTTTCATCTCACCGTTCAGGCGTTGAATTTACTTGCTGCGAATGCCAAGCATAAGGTATGGCTTAAACCACAATCTGAGATGTCTTTTTTGTATGGGAATCATGTGTTGAAGAGTGCGTTGGGTCGCATTACGGAGAATATTGTTGCTGGTGATGGGGTTGTTGTTTTTTCTATGTCGGATGTGCCTTTGGGTTTTGGGGTGGCTGCTAAGTCTACACAGGATTGTAGGAAGTTGGATCCTAATGGTATTGTTGTGCTTCATCAGGGTGATATTGGAGAGTATTTGAGGATTGAGGATGAGCTTTGA

Protein sequence

>MS.gene42324.t1

MRPLDETETSVVFEKLFKFVGNNLKNLVENPSHEGPDSNPGRYCFRLNKNKIYYCSESLVKRATNIARPNLVSLGTCIGKYTHGGNFHLTVQALNLLAANAKHKVWLKPQSEMSFLYGNHVLKSALGRITENIVAGDGVVVFSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQGDIGEYLRIEDEL