AlfalfaGEDB | MS.gene014498

M. sativa cultivar XinJiangDaYe / MS.gene014498

KEGG: K07565
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005634 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0042255 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0070925 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03657.16 PF17833.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014498.t1	XP_013462141.1	99.5	187	1	0	1	187	1	187	2.30E-103	384.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014498.t1	A4QND5	61.5	187	64	1	1	187	1	179	2.0e-60	233.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014498.t1	I3ST48	99.5	187	1	0	1	187	1	187	1.6e-103	384.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene014498	MS.gene061905	PPI
MS.gene014498	MS.gene94183	PPI
MS.gene08434	MS.gene014498	PPI
MS.gene53720	MS.gene014498	PPI
MS.gene014498	MS.gene066830	PPI
MS.gene014498	MS.gene018651	PPI
MS.gene014498	MS.gene035458	PPI
MS.gene014498	MS.gene036487	PPI
MS.gene014498	MS.gene59319	PPI
MS.gene014498	MS.gene031378	PPI
MS.gene014498	MS.gene75579	PPI
MS.gene014498	MS.gene058018	PPI
MS.gene014498	MS.gene045265	PPI
MS.gene066348	MS.gene014498	PPI
MS.gene014498	MS.gene007855	PPI
MS.gene014498	MS.gene47347	PPI
MS.gene014498	MS.gene059880	PPI
MS.gene014498	MS.gene010453	PPI
MS.gene014498	MS.gene38996	PPI
MS.gene014498	MS.gene05689	PPI
MS.gene014498	MS.gene070298	PPI
MS.gene014498	MS.gene45646	PPI
MS.gene014498	MS.gene066300	PPI
MS.gene014498	MS.gene045818	PPI
MS.gene014498	MS.gene59609	PPI
MS.gene014498	MS.gene047734	PPI
MS.gene014498	MS.gene041623	PPI
MS.gene86675	MS.gene014498	PPI
MS.gene014498	MS.gene001917	PPI
MS.gene014498	MS.gene04418	PPI
MS.gene014498	MS.gene000480	PPI
MS.gene014498	MS.gene003492	PPI
MS.gene014498	MS.gene79721	PPI
MS.gene014498	MS.gene09845	PPI
MS.gene014498	MS.gene066301	PPI
MS.gene014498	MS.gene80786	PPI
MS.gene014498	MS.gene08109	PPI
MS.gene014498	MS.gene90360	PPI
MS.gene014498	MS.gene39294	PPI
MS.gene014498	MS.gene81498	PPI
MS.gene014498	MS.gene022752	PPI
MS.gene014498	MS.gene003490	PPI
MS.gene014498	MS.gene98171	PPI
MS.gene014498	MS.gene04336	PPI
MS.gene014498	MS.gene09038	PPI
MS.gene014498	MS.gene32240	PPI
MS.gene014498	MS.gene09414	PPI
MS.gene014498	MS.gene04079	PPI
MS.gene014498	MS.gene77392	PPI
MS.gene014498	MS.gene073613	PPI
MS.gene014498	MS.gene96949	PPI
MS.gene014498	MS.gene30239	PPI
MS.gene069038	MS.gene014498	PPI
MS.gene014498	MS.gene018599	PPI
MS.gene014498	MS.gene061835	PPI
MS.gene014498	MS.gene037902	PPI
MS.gene95644	MS.gene014498	PPI
MS.gene014498	MS.gene98889	PPI
MS.gene014498	MS.gene37937	PPI
MS.gene014498	MS.gene41329	PPI
MS.gene014498	MS.gene059878	PPI
MS.gene014498	MS.gene044819	PPI
MS.gene014498	MS.gene81341	PPI
MS.gene014498	MS.gene23437	PPI
MS.gene014498	MS.gene84016	PPI
MS.gene014498	MS.gene52105	PPI
MS.gene045239	MS.gene014498	PPI
MS.gene066811	MS.gene014498	PPI
MS.gene014498	MS.gene041557	PPI
MS.gene014498	MS.gene04159	PPI
MS.gene014498	MS.gene64512	PPI
MS.gene014498	MS.gene034931	PPI
MS.gene014498	MS.gene80570	PPI
MS.gene014498	MS.gene035396	PPI
MS.gene014498	MS.gene78972	PPI
MS.gene014498	MS.gene067030	PPI
MS.gene014498	MS.gene90945	PPI
MS.gene014498	MS.gene057738	PPI
MS.gene014498	MS.gene020215	PPI
MS.gene014498	MS.gene060923	PPI
MS.gene014498	MS.gene43350	PPI
MS.gene014498	MS.gene040651	PPI
MS.gene014498	MS.gene81001	PPI
MS.gene014498	MS.gene032323	PPI
MS.gene014498	MS.gene038784	PPI
MS.gene014498	MS.gene039360	PPI
MS.gene014498	MS.gene035331	PPI
MS.gene05640	MS.gene014498	PPI
MS.gene014498	MS.gene035194	PPI
MS.gene014498	MS.gene032998	PPI
MS.gene92142	MS.gene014498	PPI
MS.gene014498	MS.gene88081	PPI
MS.gene51111	MS.gene014498	PPI
MS.gene014498	MS.gene003435	PPI
MS.gene014498	MS.gene52150	PPI
MS.gene014498	MS.gene99779	PPI
MS.gene014498	MS.gene09204	PPI
MS.gene014498	MS.gene068200	PPI
MS.gene014498	MS.gene75598	PPI
MS.gene014498	MS.gene018553	PPI
MS.gene014498	MS.gene70551	PPI
MS.gene014498	MS.gene29719	PPI
MS.gene044537	MS.gene014498	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014498.t1	MTR_3g112090	99.465	187	1	0	1	187	1	187	1.14e-139	387

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014498.t1	AT4G15770	83.422	187	31	0	1	187	1	187	3.76e-119	335

Find 45 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGATGTGCCTTTGGGTTT+TGG	0.195441	3.3:+92264972	MS.gene014498:CDS
TTCTATGTCGGATGTGCCTT+TGG	0.274575	3.3:+92264965	MS.gene014498:CDS
TGGTATTGTTGTGCTTCATC+AGG	0.357825	3.3:+92265040	MS.gene014498:CDS
GTGCTTCATCAGGGTGATAT+TGG	0.380581	3.3:+92265050	MS.gene014498:CDS
TCTATGTCGGATGTGCCTTT+GGG	0.403032	3.3:+92264966	MS.gene014498:CDS
CTAAGTCTACACAGGATTGT+AGG	0.417131	3.3:+92265003	MS.gene014498:CDS
GTGATATTGGAGAGTATTTG+AGG	0.418524	3.3:+92265063	MS.gene014498:CDS
GAGTGCGTTGGGTCGCATTA+CGG	0.440043	3.3:+92264908	MS.gene014498:CDS
CTCGTTTCTGTCTCGTCCAA+AGG	0.450814	3.3:-92264546	None:intergenic
TGGAGAGTATTTGAGGATTG+AGG	0.451876	3.3:+92265070	MS.gene014498:CDS
ATTACGGAGAATATTGTTGC+TGG	0.453018	3.3:+92264924	MS.gene014498:CDS
AGAATATTGTTGCTGGTGAT+GGG	0.475550	3.3:+92264931	MS.gene014498:CDS
GAGAATATTGTTGCTGGTGA+TGG	0.477429	3.3:+92264930	MS.gene014498:CDS
TGAAGCACAACAATACCATT+AGG	0.481187	3.3:-92265035	None:intergenic
GTTCCGAGGGAAACGAGGTT+AGG	0.488759	3.3:-92264744	None:intergenic
TACACAGGATTGTAGGAAGT+TGG	0.488772	3.3:+92265010	MS.gene014498:CDS
GTAGCGACCAGGATTTGAAT+CGG	0.501537	3.3:-92264646	None:intergenic
GTTTCCCTCGGAACGTGTAT+CGG	0.505430	3.3:+92264753	MS.gene014498:CDS
TGTAGGAAGTTGGATCCTAA+TGG	0.511689	3.3:+92265020	MS.gene014498:CDS
TTTAAGCCATACCTTATGCT+TGG	0.513488	3.3:-92264841	None:intergenic
TGCTGCGAATGCCAAGCATA+AGG	0.516412	3.3:+92264830	MS.gene014498:CDS
GAAGGTCCCGATTCAAATCC+TGG	0.525646	3.3:+92264639	MS.gene014498:CDS
GAATCGGGACCTTCGTGTGA+GGG	0.536023	3.3:-92264630	None:intergenic
AGACCTAACCTCGTTTCCCT+CGG	0.540105	3.3:+92264741	MS.gene014498:CDS
GAGGTTAGGTCTAGCGATGT+TGG	0.552615	3.3:-92264730	None:intergenic
CATGTGTTGAAGAGTGCGTT+GGG	0.555269	3.3:+92264897	MS.gene014498:CDS
TAGCGACCAGGATTTGAATC+GGG	0.568635	3.3:-92264645	None:intergenic
GAAAAGCTCTTCAAATTCGT+AGG	0.571649	3.3:+92264579	MS.gene014498:CDS
CGAATGCCAAGCATAAGGTA+TGG	0.574051	3.3:+92264835	MS.gene014498:CDS
TCATGTGTTGAAGAGTGCGT+TGG	0.587350	3.3:+92264896	MS.gene014498:CDS
AGACGGAAACAGTAGCGACC+AGG	0.591481	3.3:-92264657	None:intergenic
GGTATTGTTGTGCTTCATCA+GGG	0.595232	3.3:+92265041	MS.gene014498:CDS
TGTTGGTGGCACGCTTAACG+AGG	0.595506	3.3:-92264713	None:intergenic
GTTAGGTCTAGCGATGTTGG+TGG	0.599334	3.3:-92264727	None:intergenic
TACACGTTCCGAGGGAAACG+AGG	0.615959	3.3:-92264749	None:intergenic
TGAATCGGGACCTTCGTGTG+AGG	0.619897	3.3:-92264631	None:intergenic
AAGTAAATTCAACGCCTGAA+CGG	0.628881	3.3:-92264808	None:intergenic
GAATATTGTTGCTGGTGATG+GGG	0.643723	3.3:+92264932	MS.gene014498:CDS
GTTGGTGGCACGCTTAACGA+GGG	0.651920	3.3:-92264712	None:intergenic
GGTGGCTGCTAAGTCTACAC+AGG	0.655794	3.3:+92264995	MS.gene014498:CDS
GCAGCCACCCCAAAACCCAA+AGG	0.658414	3.3:-92264981	None:intergenic
GTTGAAAATCCCTCACACGA+AGG	0.698320	3.3:+92264621	MS.gene014498:CDS
GGACGAGACAGAAACGAGCG+TGG	0.721090	3.3:+92264551	MS.gene014498:CDS
TGTATCGGAAAATACACACA+CGG	0.738380	3.3:+92264768	MS.gene014498:CDS
ATCGGAAAATACACACACGG+TGG	0.745885	3.3:+92264771	MS.gene014498:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAGACATCTCAGATTG+TGG	-	chr3.3:92264867-92264886	None:intergenic	30.0%
!	AGATTTTGTTCTTGTTGAGA+CGG	-	chr3.3:92264677-92264696	None:intergenic	30.0%
!!!	CTGAGATGTCTTTTTTGTAT+GGG	+	chr3.3:92264871-92264890	MS.gene014498:CDS	30.0%
!!!	GGTTGTTGTTTTTTCTATGT+CGG	+	chr3.3:92264953-92264972	MS.gene014498:CDS	30.0%
!!!	TCTGAGATGTCTTTTTTGTA+TGG	+	chr3.3:92264870-92264889	MS.gene014498:CDS	30.0%
	AAGTAAATTCAACGCCTGAA+CGG	-	chr3.3:92264811-92264830	None:intergenic	35.0%
	AGAATATTGTTGCTGGTGAT+GGG	+	chr3.3:92264931-92264950	MS.gene014498:CDS	35.0%
	ATTACGGAGAATATTGTTGC+TGG	+	chr3.3:92264924-92264943	MS.gene014498:CDS	35.0%
	GAAAAGCTCTTCAAATTCGT+AGG	+	chr3.3:92264579-92264598	MS.gene014498:CDS	35.0%
	TGAAGCACAACAATACCATT+AGG	-	chr3.3:92265038-92265057	None:intergenic	35.0%
	TGTATCGGAAAATACACACA+CGG	+	chr3.3:92264768-92264787	MS.gene014498:CDS	35.0%
	TTTAAGCCATACCTTATGCT+TGG	-	chr3.3:92264844-92264863	None:intergenic	35.0%
!	AAATTTTCATCTCACCGTTC+AGG	+	chr3.3:92264794-92264813	MS.gene014498:CDS	35.0%
!!	GTGATATTGGAGAGTATTTG+AGG	+	chr3.3:92265063-92265082	MS.gene014498:CDS	35.0%
	CTAAGTCTACACAGGATTGT+AGG	+	chr3.3:92265003-92265022	MS.gene014498:CDS	40.0%
	GAGAATATTGTTGCTGGTGA+TGG	+	chr3.3:92264930-92264949	MS.gene014498:CDS	40.0%
	GGTATTGTTGTGCTTCATCA+GGG	+	chr3.3:92265041-92265060	MS.gene014498:CDS	40.0%
	TACACAGGATTGTAGGAAGT+TGG	+	chr3.3:92265010-92265029	MS.gene014498:CDS	40.0%
	TGGTATTGTTGTGCTTCATC+AGG	+	chr3.3:92265040-92265059	MS.gene014498:CDS	40.0%
	TGTAGGAAGTTGGATCCTAA+TGG	+	chr3.3:92265020-92265039	MS.gene014498:CDS	40.0%
!	GAATATTGTTGCTGGTGATG+GGG	+	chr3.3:92264932-92264951	MS.gene014498:CDS	40.0%
!!	TGGAGAGTATTTGAGGATTG+AGG	+	chr3.3:92265070-92265089	MS.gene014498:CDS	40.0%
	ATCGGAAAATACACACACGG+TGG	+	chr3.3:92264771-92264790	MS.gene014498:CDS	45.0%
	CGAATGCCAAGCATAAGGTA+TGG	+	chr3.3:92264835-92264854	MS.gene014498:CDS	45.0%
	GTAGCGACCAGGATTTGAAT+CGG	-	chr3.3:92264649-92264668	None:intergenic	45.0%
	GTGCTTCATCAGGGTGATAT+TGG	+	chr3.3:92265050-92265069	MS.gene014498:CDS	45.0%
	GTTGAAAATCCCTCACACGA+AGG	+	chr3.3:92264621-92264640	MS.gene014498:CDS	45.0%
	TAGCGACCAGGATTTGAATC+GGG	-	chr3.3:92264648-92264667	None:intergenic	45.0%
	TCTATGTCGGATGTGCCTTT+GGG	+	chr3.3:92264966-92264985	MS.gene014498:CDS	45.0%
	TTCTATGTCGGATGTGCCTT+TGG	+	chr3.3:92264965-92264984	MS.gene014498:CDS	45.0%
!	ATTTTCCGATACACGTTCCG+AGG	-	chr3.3:92264761-92264780	None:intergenic	45.0%
!	CATGTGTTGAAGAGTGCGTT+GGG	+	chr3.3:92264897-92264916	MS.gene014498:CDS	45.0%
!	TCATGTGTTGAAGAGTGCGT+TGG	+	chr3.3:92264896-92264915	MS.gene014498:CDS	45.0%
!	TTTTCCGATACACGTTCCGA+GGG	-	chr3.3:92264760-92264779	None:intergenic	45.0%
	AGACCTAACCTCGTTTCCCT+CGG	+	chr3.3:92264741-92264760	MS.gene014498:CDS	50.0%
	CTCGTTTCTGTCTCGTCCAA+AGG	-	chr3.3:92264549-92264568	None:intergenic	50.0%
	GAAGGTCCCGATTCAAATCC+TGG	+	chr3.3:92264639-92264658	MS.gene014498:CDS	50.0%
	GAGGTTAGGTCTAGCGATGT+TGG	-	chr3.3:92264733-92264752	None:intergenic	50.0%
	GTTAGGTCTAGCGATGTTGG+TGG	-	chr3.3:92264730-92264749	None:intergenic	50.0%
	GTTTCCCTCGGAACGTGTAT+CGG	+	chr3.3:92264753-92264772	MS.gene014498:CDS	50.0%
	TGCTGCGAATGCCAAGCATA+AGG	+	chr3.3:92264830-92264849	MS.gene014498:CDS	50.0%
!	CGTGTGAGGGATTTTCAACG+AGG	-	chr3.3:92264620-92264639	None:intergenic	50.0%
!!	CGGATGTGCCTTTGGGTTTT+GGG	+	chr3.3:92264973-92264992	MS.gene014498:CDS	50.0%
!!	GGATGTGCCTTTGGGTTTTG+GGG	+	chr3.3:92264974-92264993	MS.gene014498:CDS	50.0%
!!	TCGGATGTGCCTTTGGGTTT+TGG	+	chr3.3:92264972-92264991	MS.gene014498:CDS	50.0%
	AGACGGAAACAGTAGCGACC+AGG	-	chr3.3:92264660-92264679	None:intergenic	55.0%
	GAATCGGGACCTTCGTGTGA+GGG	-	chr3.3:92264633-92264652	None:intergenic	55.0%
	GAGTGCGTTGGGTCGCATTA+CGG	+	chr3.3:92264908-92264927	MS.gene014498:CDS	55.0%
	GGTGGCTGCTAAGTCTACAC+AGG	+	chr3.3:92264995-92265014	MS.gene014498:CDS	55.0%
	GTTCCGAGGGAAACGAGGTT+AGG	-	chr3.3:92264747-92264766	None:intergenic	55.0%
	TACACGTTCCGAGGGAAACG+AGG	-	chr3.3:92264752-92264771	None:intergenic	55.0%
	TGAATCGGGACCTTCGTGTG+AGG	-	chr3.3:92264634-92264653	None:intergenic	55.0%
!!	GTTGGTGGCACGCTTAACGA+GGG	-	chr3.3:92264715-92264734	None:intergenic	55.0%
!!	TGTTGGTGGCACGCTTAACG+AGG	-	chr3.3:92264716-92264735	None:intergenic	55.0%
!!!	TGTGCCTTTGGGTTTTGGGG+TGG	+	chr3.3:92264977-92264996	MS.gene014498:CDS	55.0%
	GCAGCCACCCCAAAACCCAA+AGG	-	chr3.3:92264984-92265003	None:intergenic	60.0%
	GGACGAGACAGAAACGAGCG+TGG	+	chr3.3:92264551-92264570	MS.gene014498:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	92264540	92265103	92264540	ID=MS.gene014498
chr3.3	mRNA	92264540	92265103	92264540	ID=MS.gene014498.t1;Parent=MS.gene014498
chr3.3	exon	92264540	92265103	92264540	ID=MS.gene014498.t1.exon1;Parent=MS.gene014498.t1
chr3.3	CDS	92264540	92265103	92264540	ID=cds.MS.gene014498.t1;Parent=MS.gene014498.t1

Gene Sequence

>MS.gene014498

ATGAGACCTTTGGACGAGACAGAAACGAGCGTGGTATTCGAAAAGCTCTTCAAATTCGTAGGAAACAATCTCAAAAACCTCGTTGAAAATCCCTCACACGAAGGTCCCGATTCAAATCCTGGTCGCTACTGTTTCCGTCTCAACAAGAACAAAATCTACTACTGCAGTGAATCCCTCGTTAAGCGTGCCACCAACATCGCTAGACCTAACCTCGTTTCCCTCGGAACGTGTATCGGAAAATACACACACGGTGGAAATTTTCATCTCACCGTTCAGGCGTTGAATTTACTTGCTGCGAATGCCAAGCATAAGGTATGGCTTAAACCACAATCTGAGATGTCTTTTTTGTATGGGAATCATGTGTTGAAGAGTGCGTTGGGTCGCATTACGGAGAATATTGTTGCTGGTGATGGGGTTGTTGTTTTTTCTATGTCGGATGTGCCTTTGGGTTTTGGGGTGGCTGCTAAGTCTACACAGGATTGTAGGAAGTTGGATCCTAATGGTATTGTTGTGCTTCATCAGGGTGATATTGGAGAGTATTTGAGGATTGAGGATGAGCTTTGA

Protein sequence

>MS.gene014498.t1

MRPLDETETSVVFEKLFKFVGNNLKNLVENPSHEGPDSNPGRYCFRLNKNKIYYCSESLVKRATNIARPNLVSLGTCIGKYTHGGNFHLTVQALNLLAANAKHKVWLKPQSEMSFLYGNHVLKSALGRITENIVAGDGVVVFSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQGDIGEYLRIEDEL