AlfalfaGEDB | MS.gene014499

M. sativa cultivar XinJiangDaYe / MS.gene014499

KEGG: K07565
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005634 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0042255 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0070925 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03657.16 PF17833.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014499.t1	XP_013462141.1	99.5	187	1	0	1	187	1	187	2.30E-103	384.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014499.t1	A4QND5	61.5	187	64	1	1	187	1	179	2.0e-60	233.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014499.t1	I3ST48	99.5	187	1	0	1	187	1	187	1.6e-103	384.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene014499	MS.gene035331	PPI
MS.gene52150	MS.gene014499	PPI
MS.gene04336	MS.gene014499	PPI
MS.gene044537	MS.gene014499	PPI
MS.gene70551	MS.gene014499	PPI
MS.gene061905	MS.gene014499	PPI
MS.gene014499	MS.gene09204	PPI
MS.gene020215	MS.gene014499	PPI
MS.gene014499	MS.gene045818	PPI
MS.gene014499	MS.gene59609	PPI
MS.gene04159	MS.gene014499	PPI
MS.gene81001	MS.gene014499	PPI
MS.gene066348	MS.gene014499	PPI
MS.gene038784	MS.gene014499	PPI
MS.gene035396	MS.gene014499	PPI
MS.gene04079	MS.gene014499	PPI
MS.gene37937	MS.gene014499	PPI
MS.gene09038	MS.gene014499	PPI
MS.gene014499	MS.gene000480	PPI
MS.gene05689	MS.gene014499	PPI
MS.gene070298	MS.gene014499	PPI
MS.gene014499	MS.gene035458	PPI
MS.gene039360	MS.gene014499	PPI
MS.gene073613	MS.gene014499	PPI
MS.gene067030	MS.gene014499	PPI
MS.gene014499	MS.gene90360	PPI
MS.gene032998	MS.gene014499	PPI
MS.gene80570	MS.gene014499	PPI
MS.gene047734	MS.gene014499	PPI
MS.gene034931	MS.gene014499	PPI
MS.gene98889	MS.gene014499	PPI
MS.gene059880	MS.gene014499	PPI
MS.gene94183	MS.gene014499	PPI
MS.gene014499	MS.gene98171	PPI
MS.gene003492	MS.gene014499	PPI
MS.gene018651	MS.gene014499	PPI
MS.gene88081	MS.gene014499	PPI
MS.gene035194	MS.gene014499	PPI
MS.gene38996	MS.gene014499	PPI
MS.gene037902	MS.gene014499	PPI
MS.gene81341	MS.gene014499	PPI
MS.gene014499	MS.gene77392	PPI
MS.gene43350	MS.gene014499	PPI
MS.gene86675	MS.gene014499	PPI
MS.gene08109	MS.gene014499	PPI
MS.gene80786	MS.gene014499	PPI
MS.gene014499	MS.gene96949	PPI
MS.gene79721	MS.gene014499	PPI
MS.gene061835	MS.gene014499	PPI
MS.gene30239	MS.gene014499	PPI
MS.gene045265	MS.gene014499	PPI
MS.gene09845	MS.gene014499	PPI
MS.gene014499	MS.gene001917	PPI
MS.gene32240	MS.gene014499	PPI
MS.gene036487	MS.gene014499	PPI
MS.gene018599	MS.gene014499	PPI
MS.gene022752	MS.gene014499	PPI
MS.gene018553	MS.gene014499	PPI
MS.gene014499	MS.gene066301	PPI
MS.gene066300	MS.gene014499	PPI
MS.gene39294	MS.gene014499	PPI
MS.gene060923	MS.gene014499	PPI
MS.gene23437	MS.gene014499	PPI
MS.gene069038	MS.gene014499	PPI
MS.gene014499	MS.gene64512	PPI
MS.gene45646	MS.gene014499	PPI
MS.gene041623	MS.gene014499	PPI
MS.gene010453	MS.gene014499	PPI
MS.gene41329	MS.gene014499	PPI
MS.gene059878	MS.gene014499	PPI
MS.gene95644	MS.gene014499	PPI
MS.gene040651	MS.gene014499	PPI
MS.gene068200	MS.gene014499	PPI
MS.gene52105	MS.gene014499	PPI
MS.gene045239	MS.gene014499	PPI
MS.gene066811	MS.gene014499	PPI
MS.gene90945	MS.gene014499	PPI
MS.gene59319	MS.gene014499	PPI
MS.gene031378	MS.gene014499	PPI
MS.gene014499	MS.gene044819	PPI
MS.gene04418	MS.gene014499	PPI
MS.gene99779	MS.gene014499	PPI
MS.gene058018	MS.gene014499	PPI
MS.gene003435	MS.gene014499	PPI
MS.gene47347	MS.gene014499	PPI
MS.gene08434	MS.gene014499	PPI
MS.gene53720	MS.gene014499	PPI
MS.gene014499	MS.gene75598	PPI
MS.gene014499	MS.gene041557	PPI
MS.gene003490	MS.gene014499	PPI
MS.gene29719	MS.gene014499	PPI
MS.gene78972	MS.gene014499	PPI
MS.gene84016	MS.gene014499	PPI
MS.gene014499	MS.gene057738	PPI
MS.gene014499	MS.gene066830	PPI
MS.gene09414	MS.gene014499	PPI
MS.gene007855	MS.gene014499	PPI
MS.gene05640	MS.gene014499	PPI
MS.gene92142	MS.gene014499	PPI
MS.gene51111	MS.gene014499	PPI
MS.gene014499	MS.gene75579	PPI
MS.gene81498	MS.gene014499	PPI
MS.gene032323	MS.gene014499	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014499.t1	MTR_3g112090	99.465	187	1	0	1	187	1	187	1.14e-139	387

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014499.t1	AT4G15770	83.422	187	31	0	1	187	1	187	3.76e-119	335

Find 45 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGATGTGCCTTTGGGTTT+TGG	0.195441	3.1:+89446939	MS.gene014499:CDS
TTCTATGTCGGATGTGCCTT+TGG	0.274575	3.1:+89446932	MS.gene014499:CDS
TGGTATTGTTGTGCTTCATC+AGG	0.357825	3.1:+89447007	MS.gene014499:CDS
GTGCTTCATCAGGGTGATAT+TGG	0.380581	3.1:+89447017	MS.gene014499:CDS
TCTATGTCGGATGTGCCTTT+GGG	0.403032	3.1:+89446933	MS.gene014499:CDS
CTAAGTCTACACAGGATTGT+AGG	0.417131	3.1:+89446970	MS.gene014499:CDS
GTGATATTGGAGAGTATTTG+AGG	0.418524	3.1:+89447030	MS.gene014499:CDS
GAGTGCGTTGGGTCGCATTA+CGG	0.440043	3.1:+89446875	MS.gene014499:CDS
CTCGTTTCTGTCTCGTCCAA+AGG	0.450814	3.1:-89446513	None:intergenic
TGGAGAGTATTTGAGGATTG+AGG	0.451876	3.1:+89447037	MS.gene014499:CDS
ATTACGGAGAATATTGTTGC+TGG	0.453018	3.1:+89446891	MS.gene014499:CDS
AGAATATTGTTGCTGGTGAT+GGG	0.475550	3.1:+89446898	MS.gene014499:CDS
GAGAATATTGTTGCTGGTGA+TGG	0.477429	3.1:+89446897	MS.gene014499:CDS
TGAAGCACAACAATACCATT+AGG	0.481187	3.1:-89447002	None:intergenic
GTTCCGAGGGAAACGAGGTT+AGG	0.488759	3.1:-89446711	None:intergenic
TACACAGGATTGTAGGAAGT+TGG	0.488772	3.1:+89446977	MS.gene014499:CDS
GTAGCGACCAGGATTTGAAT+CGG	0.501537	3.1:-89446613	None:intergenic
GTTTCCCTCGGAACGTGTAT+CGG	0.505430	3.1:+89446720	MS.gene014499:CDS
TGTAGGAAGTTGGATCCTAA+TGG	0.511689	3.1:+89446987	MS.gene014499:CDS
TTTAAGCCATACCTTATGCT+TGG	0.513488	3.1:-89446808	None:intergenic
TGCTGCGAATGCCAAGCATA+AGG	0.516412	3.1:+89446797	MS.gene014499:CDS
GAAGGTCCCGATTCAAATCC+TGG	0.525646	3.1:+89446606	MS.gene014499:CDS
GAATCGGGACCTTCGTGTGA+GGG	0.536023	3.1:-89446597	None:intergenic
AGACCTAACCTCGTTTCCCT+CGG	0.540105	3.1:+89446708	MS.gene014499:CDS
GAGGTTAGGTCTAGCGATGT+TGG	0.552615	3.1:-89446697	None:intergenic
CATGTGTTGAAGAGTGCGTT+GGG	0.555269	3.1:+89446864	MS.gene014499:CDS
TAGCGACCAGGATTTGAATC+GGG	0.568635	3.1:-89446612	None:intergenic
GAAAAGCTCTTCAAATTCGT+AGG	0.571649	3.1:+89446546	MS.gene014499:CDS
CGAATGCCAAGCATAAGGTA+TGG	0.574051	3.1:+89446802	MS.gene014499:CDS
TCATGTGTTGAAGAGTGCGT+TGG	0.587350	3.1:+89446863	MS.gene014499:CDS
AGACGGAAACAGTAGCGACC+AGG	0.591481	3.1:-89446624	None:intergenic
GGTATTGTTGTGCTTCATCA+GGG	0.595232	3.1:+89447008	MS.gene014499:CDS
TGTTGGTGGCACGCTTAACG+AGG	0.595506	3.1:-89446680	None:intergenic
GTTAGGTCTAGCGATGTTGG+TGG	0.599334	3.1:-89446694	None:intergenic
TACACGTTCCGAGGGAAACG+AGG	0.615959	3.1:-89446716	None:intergenic
TGAATCGGGACCTTCGTGTG+AGG	0.619897	3.1:-89446598	None:intergenic
AAGTAAATTCAACGCCTGAA+CGG	0.628881	3.1:-89446775	None:intergenic
GAATATTGTTGCTGGTGATG+GGG	0.643723	3.1:+89446899	MS.gene014499:CDS
GTTGGTGGCACGCTTAACGA+GGG	0.651920	3.1:-89446679	None:intergenic
GGTGGCTGCTAAGTCTACAC+AGG	0.655794	3.1:+89446962	MS.gene014499:CDS
GCAGCCACCCCAAAACCCAA+AGG	0.658414	3.1:-89446948	None:intergenic
GTTGAAAATCCCTCACACGA+AGG	0.698320	3.1:+89446588	MS.gene014499:CDS
GGACGAGACAGAAACGAGCG+TGG	0.721090	3.1:+89446518	MS.gene014499:CDS
TGTATCGGAAAATACACACA+CGG	0.738380	3.1:+89446735	MS.gene014499:CDS
ATCGGAAAATACACACACGG+TGG	0.745885	3.1:+89446738	MS.gene014499:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAGACATCTCAGATTG+TGG	-	chr3.1:89446834-89446853	None:intergenic	30.0%
!	AGATTTTGTTCTTGTTGAGA+CGG	-	chr3.1:89446644-89446663	None:intergenic	30.0%
!!!	CTGAGATGTCTTTTTTGTAT+GGG	+	chr3.1:89446838-89446857	MS.gene014499:CDS	30.0%
!!!	GGTTGTTGTTTTTTCTATGT+CGG	+	chr3.1:89446920-89446939	MS.gene014499:CDS	30.0%
!!!	TCTGAGATGTCTTTTTTGTA+TGG	+	chr3.1:89446837-89446856	MS.gene014499:CDS	30.0%
	AAGTAAATTCAACGCCTGAA+CGG	-	chr3.1:89446778-89446797	None:intergenic	35.0%
	AGAATATTGTTGCTGGTGAT+GGG	+	chr3.1:89446898-89446917	MS.gene014499:CDS	35.0%
	ATTACGGAGAATATTGTTGC+TGG	+	chr3.1:89446891-89446910	MS.gene014499:CDS	35.0%
	GAAAAGCTCTTCAAATTCGT+AGG	+	chr3.1:89446546-89446565	MS.gene014499:CDS	35.0%
	TGAAGCACAACAATACCATT+AGG	-	chr3.1:89447005-89447024	None:intergenic	35.0%
	TGTATCGGAAAATACACACA+CGG	+	chr3.1:89446735-89446754	MS.gene014499:CDS	35.0%
	TTTAAGCCATACCTTATGCT+TGG	-	chr3.1:89446811-89446830	None:intergenic	35.0%
!	AAATTTTCATCTCACCGTTC+AGG	+	chr3.1:89446761-89446780	MS.gene014499:CDS	35.0%
!!	GTGATATTGGAGAGTATTTG+AGG	+	chr3.1:89447030-89447049	MS.gene014499:CDS	35.0%
	CTAAGTCTACACAGGATTGT+AGG	+	chr3.1:89446970-89446989	MS.gene014499:CDS	40.0%
	GAGAATATTGTTGCTGGTGA+TGG	+	chr3.1:89446897-89446916	MS.gene014499:CDS	40.0%
	GGTATTGTTGTGCTTCATCA+GGG	+	chr3.1:89447008-89447027	MS.gene014499:CDS	40.0%
	TACACAGGATTGTAGGAAGT+TGG	+	chr3.1:89446977-89446996	MS.gene014499:CDS	40.0%
	TGGTATTGTTGTGCTTCATC+AGG	+	chr3.1:89447007-89447026	MS.gene014499:CDS	40.0%
	TGTAGGAAGTTGGATCCTAA+TGG	+	chr3.1:89446987-89447006	MS.gene014499:CDS	40.0%
!	GAATATTGTTGCTGGTGATG+GGG	+	chr3.1:89446899-89446918	MS.gene014499:CDS	40.0%
!!	TGGAGAGTATTTGAGGATTG+AGG	+	chr3.1:89447037-89447056	MS.gene014499:CDS	40.0%
	ATCGGAAAATACACACACGG+TGG	+	chr3.1:89446738-89446757	MS.gene014499:CDS	45.0%
	CGAATGCCAAGCATAAGGTA+TGG	+	chr3.1:89446802-89446821	MS.gene014499:CDS	45.0%
	GTAGCGACCAGGATTTGAAT+CGG	-	chr3.1:89446616-89446635	None:intergenic	45.0%
	GTGCTTCATCAGGGTGATAT+TGG	+	chr3.1:89447017-89447036	MS.gene014499:CDS	45.0%
	GTTGAAAATCCCTCACACGA+AGG	+	chr3.1:89446588-89446607	MS.gene014499:CDS	45.0%
	TAGCGACCAGGATTTGAATC+GGG	-	chr3.1:89446615-89446634	None:intergenic	45.0%
	TCTATGTCGGATGTGCCTTT+GGG	+	chr3.1:89446933-89446952	MS.gene014499:CDS	45.0%
	TTCTATGTCGGATGTGCCTT+TGG	+	chr3.1:89446932-89446951	MS.gene014499:CDS	45.0%
!	ATTTTCCGATACACGTTCCG+AGG	-	chr3.1:89446728-89446747	None:intergenic	45.0%
!	CATGTGTTGAAGAGTGCGTT+GGG	+	chr3.1:89446864-89446883	MS.gene014499:CDS	45.0%
!	TCATGTGTTGAAGAGTGCGT+TGG	+	chr3.1:89446863-89446882	MS.gene014499:CDS	45.0%
!	TTTTCCGATACACGTTCCGA+GGG	-	chr3.1:89446727-89446746	None:intergenic	45.0%
	AGACCTAACCTCGTTTCCCT+CGG	+	chr3.1:89446708-89446727	MS.gene014499:CDS	50.0%
	CTCGTTTCTGTCTCGTCCAA+AGG	-	chr3.1:89446516-89446535	None:intergenic	50.0%
	GAAGGTCCCGATTCAAATCC+TGG	+	chr3.1:89446606-89446625	MS.gene014499:CDS	50.0%
	GAGGTTAGGTCTAGCGATGT+TGG	-	chr3.1:89446700-89446719	None:intergenic	50.0%
	GTTAGGTCTAGCGATGTTGG+TGG	-	chr3.1:89446697-89446716	None:intergenic	50.0%
	GTTTCCCTCGGAACGTGTAT+CGG	+	chr3.1:89446720-89446739	MS.gene014499:CDS	50.0%
	TGCTGCGAATGCCAAGCATA+AGG	+	chr3.1:89446797-89446816	MS.gene014499:CDS	50.0%
!	CGTGTGAGGGATTTTCAACG+AGG	-	chr3.1:89446587-89446606	None:intergenic	50.0%
!!	CGGATGTGCCTTTGGGTTTT+GGG	+	chr3.1:89446940-89446959	MS.gene014499:CDS	50.0%
!!	GGATGTGCCTTTGGGTTTTG+GGG	+	chr3.1:89446941-89446960	MS.gene014499:CDS	50.0%
!!	TCGGATGTGCCTTTGGGTTT+TGG	+	chr3.1:89446939-89446958	MS.gene014499:CDS	50.0%
	AGACGGAAACAGTAGCGACC+AGG	-	chr3.1:89446627-89446646	None:intergenic	55.0%
	GAATCGGGACCTTCGTGTGA+GGG	-	chr3.1:89446600-89446619	None:intergenic	55.0%
	GAGTGCGTTGGGTCGCATTA+CGG	+	chr3.1:89446875-89446894	MS.gene014499:CDS	55.0%
	GGTGGCTGCTAAGTCTACAC+AGG	+	chr3.1:89446962-89446981	MS.gene014499:CDS	55.0%
	GTTCCGAGGGAAACGAGGTT+AGG	-	chr3.1:89446714-89446733	None:intergenic	55.0%
	TACACGTTCCGAGGGAAACG+AGG	-	chr3.1:89446719-89446738	None:intergenic	55.0%
	TGAATCGGGACCTTCGTGTG+AGG	-	chr3.1:89446601-89446620	None:intergenic	55.0%
!!	GTTGGTGGCACGCTTAACGA+GGG	-	chr3.1:89446682-89446701	None:intergenic	55.0%
!!	TGTTGGTGGCACGCTTAACG+AGG	-	chr3.1:89446683-89446702	None:intergenic	55.0%
!!!	TGTGCCTTTGGGTTTTGGGG+TGG	+	chr3.1:89446944-89446963	MS.gene014499:CDS	55.0%
	GCAGCCACCCCAAAACCCAA+AGG	-	chr3.1:89446951-89446970	None:intergenic	60.0%
	GGACGAGACAGAAACGAGCG+TGG	+	chr3.1:89446518-89446537	MS.gene014499:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	89446507	89447070	89446507	ID=MS.gene014499
chr3.1	mRNA	89446507	89447070	89446507	ID=MS.gene014499.t1;Parent=MS.gene014499
chr3.1	exon	89446507	89447070	89446507	ID=MS.gene014499.t1.exon1;Parent=MS.gene014499.t1
chr3.1	CDS	89446507	89447070	89446507	ID=cds.MS.gene014499.t1;Parent=MS.gene014499.t1

Gene Sequence

>MS.gene014499

ATGAGACCTTTGGACGAGACAGAAACGAGCGTGGTATTCGAAAAGCTCTTCAAATTCGTAGGAAACAATCTCAAAAACCTCGTTGAAAATCCCTCACACGAAGGTCCCGATTCAAATCCTGGTCGCTACTGTTTCCGTCTCAACAAGAACAAAATCTACTACTGCAGTGAATCCCTCGTTAAGCGTGCCACCAACATCGCTAGACCTAACCTCGTTTCCCTCGGAACGTGTATCGGAAAATACACACACGGTGGAAATTTTCATCTCACCGTTCAGGCGTTGAATTTACTTGCTGCGAATGCCAAGCATAAGGTATGGCTTAAACCACAATCTGAGATGTCTTTTTTGTATGGGAATCATGTGTTGAAGAGTGCGTTGGGTCGCATTACGGAGAATATTGTTGCTGGTGATGGGGTTGTTGTTTTTTCTATGTCGGATGTGCCTTTGGGTTTTGGGGTGGCTGCTAAGTCTACACAGGATTGTAGGAAGTTGGATCCTAATGGTATTGTTGTGCTTCATCAGGGTGATATTGGAGAGTATTTGAGGATTGAGGATGAGCTTTGA

Protein sequence

>MS.gene014499.t1

MRPLDETETSVVFEKLFKFVGNNLKNLVENPSHEGPDSNPGRYCFRLNKNKIYYCSESLVKRATNIARPNLVSLGTCIGKYTHGGNFHLTVQALNLLAANAKHKVWLKPQSEMSFLYGNHVLKSALGRITENIVAGDGVVVFSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQGDIGEYLRIEDEL