AlfalfaGEDB | MS.gene064682

M. sativa cultivar XinJiangDaYe / MS.gene064682

KEGG: K07565
GO: GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005634 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0042255 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0070925 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03657.16 PF17833.4

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064682.t1	XP_013462141.1	99.5	187	1	0	1	187	1	187	2.30E-103	384.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064682.t1	A4QND5	61.5	187	64	1	1	187	1	179	2.0e-60	233.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064682.t1	I3ST48	99.5	187	1	0	1	187	1	187	1.6e-103	384.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050574	MS.gene064682	0.813301	2.74E-51	-1.69E-46
MS.gene053007	MS.gene064682	0.809207	2.12E-50	-1.69E-46
MS.gene056257	MS.gene064682	0.831668	1.47E-55	-1.69E-46
MS.gene058030	MS.gene064682	0.838014	3.70E-57	-1.69E-46
MS.gene060830	MS.gene064682	0.836735	7.86E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene92142	MS.gene064682	PPI
MS.gene064682	MS.gene066301	PPI
MS.gene81498	MS.gene064682	PPI
MS.gene064682	MS.gene022752	PPI
MS.gene064682	MS.gene52105	PPI
MS.gene52150	MS.gene064682	PPI
MS.gene04336	MS.gene064682	PPI
MS.gene044537	MS.gene064682	PPI
MS.gene064682	MS.gene64512	PPI
MS.gene064682	MS.gene034931	PPI
MS.gene70551	MS.gene064682	PPI
MS.gene061905	MS.gene064682	PPI
MS.gene08434	MS.gene064682	PPI
MS.gene53720	MS.gene064682	PPI
MS.gene064682	MS.gene067030	PPI
MS.gene020215	MS.gene064682	PPI
MS.gene064682	MS.gene060923	PPI
MS.gene064682	MS.gene43350	PPI
MS.gene04159	MS.gene064682	PPI
MS.gene066348	MS.gene064682	PPI
MS.gene035396	MS.gene064682	PPI
MS.gene04079	MS.gene064682	PPI
MS.gene37937	MS.gene064682	PPI
MS.gene09038	MS.gene064682	PPI
MS.gene064682	MS.gene81001	PPI
MS.gene070298	MS.gene064682	PPI
MS.gene064682	MS.gene038784	PPI
MS.gene039360	MS.gene064682	PPI
MS.gene073613	MS.gene064682	PPI
MS.gene51111	MS.gene064682	PPI
MS.gene064682	MS.gene044819	PPI
MS.gene032998	MS.gene064682	PPI
MS.gene80570	MS.gene064682	PPI
MS.gene064682	MS.gene84016	PPI
MS.gene98889	MS.gene064682	PPI
MS.gene064682	MS.gene75598	PPI
MS.gene059880	MS.gene064682	PPI
MS.gene94183	MS.gene064682	PPI
MS.gene064682	MS.gene041557	PPI
MS.gene88081	MS.gene064682	PPI
MS.gene035194	MS.gene064682	PPI
MS.gene037902	MS.gene064682	PPI
MS.gene064682	MS.gene29719	PPI
MS.gene81341	MS.gene064682	PPI
MS.gene86675	MS.gene064682	PPI
MS.gene064682	MS.gene90945	PPI
MS.gene08109	MS.gene064682	PPI
MS.gene80786	MS.gene064682	PPI
MS.gene064682	MS.gene057738	PPI
MS.gene064682	MS.gene066830	PPI
MS.gene061835	MS.gene064682	PPI
MS.gene064682	MS.gene018651	PPI
MS.gene045265	MS.gene064682	PPI
MS.gene064682	MS.gene75579	PPI
MS.gene09845	MS.gene064682	PPI
MS.gene05689	MS.gene064682	PPI
MS.gene064682	MS.gene032323	PPI
MS.gene32240	MS.gene064682	PPI
MS.gene036487	MS.gene064682	PPI
MS.gene018599	MS.gene064682	PPI
MS.gene064682	MS.gene035331	PPI
MS.gene064682	MS.gene010453	PPI
MS.gene047734	MS.gene064682	PPI
MS.gene064682	MS.gene99779	PPI
MS.gene39294	MS.gene064682	PPI
MS.gene064682	MS.gene09204	PPI
MS.gene23437	MS.gene064682	PPI
MS.gene069038	MS.gene064682	PPI
MS.gene064682	MS.gene066300	PPI
MS.gene064682	MS.gene59609	PPI
MS.gene38996	MS.gene064682	PPI
MS.gene45646	MS.gene064682	PPI
MS.gene064682	MS.gene041623	PPI
MS.gene059878	MS.gene064682	PPI
MS.gene95644	MS.gene064682	PPI
MS.gene064682	MS.gene000480	PPI
MS.gene064682	MS.gene003492	PPI
MS.gene064682	MS.gene79721	PPI
MS.gene064682	MS.gene035458	PPI
MS.gene040651	MS.gene064682	PPI
MS.gene068200	MS.gene064682	PPI
MS.gene064682	MS.gene90360	PPI
MS.gene045239	MS.gene064682	PPI
MS.gene066811	MS.gene064682	PPI
MS.gene018553	MS.gene064682	PPI
MS.gene59319	MS.gene064682	PPI
MS.gene064682	MS.gene98171	PPI
MS.gene064682	MS.gene47347	PPI
MS.gene031378	MS.gene064682	PPI
MS.gene04418	MS.gene064682	PPI
MS.gene058018	MS.gene064682	PPI
MS.gene003435	MS.gene064682	PPI
MS.gene064682	MS.gene77392	PPI
MS.gene064682	MS.gene045818	PPI
MS.gene003490	MS.gene064682	PPI
MS.gene064682	MS.gene96949	PPI
MS.gene064682	MS.gene30239	PPI
MS.gene78972	MS.gene064682	PPI
MS.gene41329	MS.gene064682	PPI
MS.gene09414	MS.gene064682	PPI
MS.gene064682	MS.gene001917	PPI
MS.gene007855	MS.gene064682	PPI
MS.gene05640	MS.gene064682	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064682.t1	MTR_3g112090	99.465	187	1	0	1	187	1	187	1.14e-139	387

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064682.t1	AT4G15770	83.422	187	31	0	1	187	1	187	3.76e-119	335

Find 46 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGATGTGCCTTTGGGTTT+TGG	0.195441	3.4:+97083207	MS.gene064682:CDS
TTCTATGTCGGATGTGCCTT+TGG	0.274575	3.4:+97083200	MS.gene064682:CDS
TGGTATTGTTGTGCTTCATC+AGG	0.357825	3.4:+97083275	MS.gene064682:CDS
GTGCTTCATCAGGGTGATAT+TGG	0.380581	3.4:+97083285	MS.gene064682:CDS
TCTATGTCGGATGTGCCTTT+GGG	0.403032	3.4:+97083201	MS.gene064682:CDS
GTGATATTGGAGAGTATTTG+AGG	0.418524	3.4:+97083298	MS.gene064682:CDS
CCAAGTCTACACAGGATTGT+AGG	0.422709	3.4:+97083238	MS.gene064682:CDS
GAGTGCGTTGGGTCGCATTA+CGG	0.440043	3.4:+97083143	MS.gene064682:CDS
CTCGTTTCTGTCTCGTCCAA+AGG	0.450814	3.4:-97082781	None:intergenic
TGGAGAGTATTTGAGGATTG+AGG	0.451876	3.4:+97083305	MS.gene064682:CDS
ATTACGGAGAATATTGTTGC+TGG	0.453018	3.4:+97083159	MS.gene064682:CDS
AGAATATTGTTGCTGGTGAT+GGG	0.475550	3.4:+97083166	MS.gene064682:CDS
GAGAATATTGTTGCTGGTGA+TGG	0.477429	3.4:+97083165	MS.gene064682:CDS
TGAAGCACAACAATACCATT+AGG	0.481187	3.4:-97083270	None:intergenic
GTTCCGAGGGAAACGAGGTT+AGG	0.488759	3.4:-97082979	None:intergenic
TACACAGGATTGTAGGAAGT+TGG	0.488772	3.4:+97083245	MS.gene064682:CDS
GTAGCGACCAGGATTTGAAT+CGG	0.501537	3.4:-97082881	None:intergenic
GTTTCCCTCGGAACGTGTAT+CGG	0.505430	3.4:+97082988	MS.gene064682:CDS
TGTAGGAAGTTGGATCCTAA+TGG	0.511689	3.4:+97083255	MS.gene064682:CDS
TTTAAGCCATACCTTATGCT+TGG	0.513488	3.4:-97083076	None:intergenic
TGCTGCGAATGCCAAGCATA+AGG	0.516412	3.4:+97083065	MS.gene064682:CDS
GAAGGTCCCGATTCAAATCC+TGG	0.525646	3.4:+97082874	MS.gene064682:CDS
CCTACAATCCTGTGTAGACT+TGG	0.530107	3.4:-97083238	None:intergenic
GAATCGGGACCTTCGTGTGA+GGG	0.536023	3.4:-97082865	None:intergenic
AGACCTAACCTCGTTTCCCT+CGG	0.540105	3.4:+97082976	MS.gene064682:CDS
GAGGTTAGGTCTAGCGATGT+TGG	0.552615	3.4:-97082965	None:intergenic
CATGTGTTGAAGAGTGCGTT+GGG	0.555269	3.4:+97083132	MS.gene064682:CDS
TAGCGACCAGGATTTGAATC+GGG	0.568635	3.4:-97082880	None:intergenic
GAAAAGCTCTTCAAATTCGT+AGG	0.571649	3.4:+97082814	MS.gene064682:CDS
CGAATGCCAAGCATAAGGTA+TGG	0.574051	3.4:+97083070	MS.gene064682:CDS
TCATGTGTTGAAGAGTGCGT+TGG	0.587350	3.4:+97083131	MS.gene064682:CDS
AGACGGAAACAGTAGCGACC+AGG	0.591481	3.4:-97082892	None:intergenic
GGTATTGTTGTGCTTCATCA+GGG	0.595232	3.4:+97083276	MS.gene064682:CDS
TGTTGGTGGCACGCTTAACG+AGG	0.595506	3.4:-97082948	None:intergenic
GTTAGGTCTAGCGATGTTGG+TGG	0.599334	3.4:-97082962	None:intergenic
TACACGTTCCGAGGGAAACG+AGG	0.615959	3.4:-97082984	None:intergenic
TGAATCGGGACCTTCGTGTG+AGG	0.619897	3.4:-97082866	None:intergenic
GCAGCCACCCCAAAACCCAA+AGG	0.624108	3.4:-97083216	None:intergenic
AAGTAAATTCAACGCCTGAA+CGG	0.628881	3.4:-97083043	None:intergenic
GAATATTGTTGCTGGTGATG+GGG	0.643723	3.4:+97083167	MS.gene064682:CDS
GTTGGTGGCACGCTTAACGA+GGG	0.651920	3.4:-97082947	None:intergenic
GGTGGCTGCCAAGTCTACAC+AGG	0.664800	3.4:+97083230	MS.gene064682:CDS
GTTGAAAATCCCTCACACGA+AGG	0.698320	3.4:+97082856	MS.gene064682:CDS
GGACGAGACAGAAACGAGCG+TGG	0.721090	3.4:+97082786	MS.gene064682:CDS
TGTATCGGAAAATACACACA+CGG	0.738380	3.4:+97083003	MS.gene064682:CDS
ATCGGAAAATACACACACGG+TGG	0.745885	3.4:+97083006	MS.gene064682:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAAAGACATCTCAGATTG+TGG	-	chr3.4:97083102-97083121	None:intergenic	30.0%
!	AGATTTTGTTCTTGTTGAGA+CGG	-	chr3.4:97082912-97082931	None:intergenic	30.0%
!!!	CTGAGATGTCTTTTTTGTAT+GGG	+	chr3.4:97083106-97083125	MS.gene064682:CDS	30.0%
!!!	GGTTGTTGTTTTTTCTATGT+CGG	+	chr3.4:97083188-97083207	MS.gene064682:CDS	30.0%
!!!	TCTGAGATGTCTTTTTTGTA+TGG	+	chr3.4:97083105-97083124	MS.gene064682:CDS	30.0%
	AAGTAAATTCAACGCCTGAA+CGG	-	chr3.4:97083046-97083065	None:intergenic	35.0%
	AGAATATTGTTGCTGGTGAT+GGG	+	chr3.4:97083166-97083185	MS.gene064682:CDS	35.0%
	ATTACGGAGAATATTGTTGC+TGG	+	chr3.4:97083159-97083178	MS.gene064682:CDS	35.0%
	GAAAAGCTCTTCAAATTCGT+AGG	+	chr3.4:97082814-97082833	MS.gene064682:CDS	35.0%
	TGAAGCACAACAATACCATT+AGG	-	chr3.4:97083273-97083292	None:intergenic	35.0%
	TGTATCGGAAAATACACACA+CGG	+	chr3.4:97083003-97083022	MS.gene064682:CDS	35.0%
	TTTAAGCCATACCTTATGCT+TGG	-	chr3.4:97083079-97083098	None:intergenic	35.0%
!	AAATTTTCATCTCACCGTTC+AGG	+	chr3.4:97083029-97083048	MS.gene064682:CDS	35.0%
!!	GTGATATTGGAGAGTATTTG+AGG	+	chr3.4:97083298-97083317	MS.gene064682:CDS	35.0%
	GAGAATATTGTTGCTGGTGA+TGG	+	chr3.4:97083165-97083184	MS.gene064682:CDS	40.0%
	GGTATTGTTGTGCTTCATCA+GGG	+	chr3.4:97083276-97083295	MS.gene064682:CDS	40.0%
	TACACAGGATTGTAGGAAGT+TGG	+	chr3.4:97083245-97083264	MS.gene064682:CDS	40.0%
	TGGTATTGTTGTGCTTCATC+AGG	+	chr3.4:97083275-97083294	MS.gene064682:CDS	40.0%
	TGTAGGAAGTTGGATCCTAA+TGG	+	chr3.4:97083255-97083274	MS.gene064682:CDS	40.0%
!	GAATATTGTTGCTGGTGATG+GGG	+	chr3.4:97083167-97083186	MS.gene064682:CDS	40.0%
!!	TGGAGAGTATTTGAGGATTG+AGG	+	chr3.4:97083305-97083324	MS.gene064682:CDS	40.0%
	ATCGGAAAATACACACACGG+TGG	+	chr3.4:97083006-97083025	MS.gene064682:CDS	45.0%
	CCAAGTCTACACAGGATTGT+AGG	+	chr3.4:97083238-97083257	MS.gene064682:CDS	45.0%
	CCTACAATCCTGTGTAGACT+TGG	-	chr3.4:97083241-97083260	None:intergenic	45.0%
	CGAATGCCAAGCATAAGGTA+TGG	+	chr3.4:97083070-97083089	MS.gene064682:CDS	45.0%
	GTAGCGACCAGGATTTGAAT+CGG	-	chr3.4:97082884-97082903	None:intergenic	45.0%
	GTGCTTCATCAGGGTGATAT+TGG	+	chr3.4:97083285-97083304	MS.gene064682:CDS	45.0%
	GTTGAAAATCCCTCACACGA+AGG	+	chr3.4:97082856-97082875	MS.gene064682:CDS	45.0%
	TAGCGACCAGGATTTGAATC+GGG	-	chr3.4:97082883-97082902	None:intergenic	45.0%
	TCTATGTCGGATGTGCCTTT+GGG	+	chr3.4:97083201-97083220	MS.gene064682:CDS	45.0%
	TTCTATGTCGGATGTGCCTT+TGG	+	chr3.4:97083200-97083219	MS.gene064682:CDS	45.0%
!	ATTTTCCGATACACGTTCCG+AGG	-	chr3.4:97082996-97083015	None:intergenic	45.0%
!	CATGTGTTGAAGAGTGCGTT+GGG	+	chr3.4:97083132-97083151	MS.gene064682:CDS	45.0%
!	TCATGTGTTGAAGAGTGCGT+TGG	+	chr3.4:97083131-97083150	MS.gene064682:CDS	45.0%
!	TTTTCCGATACACGTTCCGA+GGG	-	chr3.4:97082995-97083014	None:intergenic	45.0%
	AGACCTAACCTCGTTTCCCT+CGG	+	chr3.4:97082976-97082995	MS.gene064682:CDS	50.0%
	CTCGTTTCTGTCTCGTCCAA+AGG	-	chr3.4:97082784-97082803	None:intergenic	50.0%
	GAAGGTCCCGATTCAAATCC+TGG	+	chr3.4:97082874-97082893	MS.gene064682:CDS	50.0%
	GAGGTTAGGTCTAGCGATGT+TGG	-	chr3.4:97082968-97082987	None:intergenic	50.0%
	GTTAGGTCTAGCGATGTTGG+TGG	-	chr3.4:97082965-97082984	None:intergenic	50.0%
	GTTTCCCTCGGAACGTGTAT+CGG	+	chr3.4:97082988-97083007	MS.gene064682:CDS	50.0%
	TGCTGCGAATGCCAAGCATA+AGG	+	chr3.4:97083065-97083084	MS.gene064682:CDS	50.0%
!	CGTGTGAGGGATTTTCAACG+AGG	-	chr3.4:97082855-97082874	None:intergenic	50.0%
!!	CGGATGTGCCTTTGGGTTTT+GGG	+	chr3.4:97083208-97083227	MS.gene064682:CDS	50.0%
!!	GGATGTGCCTTTGGGTTTTG+GGG	+	chr3.4:97083209-97083228	MS.gene064682:CDS	50.0%
!!	TCGGATGTGCCTTTGGGTTT+TGG	+	chr3.4:97083207-97083226	MS.gene064682:CDS	50.0%
	AGACGGAAACAGTAGCGACC+AGG	-	chr3.4:97082895-97082914	None:intergenic	55.0%
	GAATCGGGACCTTCGTGTGA+GGG	-	chr3.4:97082868-97082887	None:intergenic	55.0%
	GAGTGCGTTGGGTCGCATTA+CGG	+	chr3.4:97083143-97083162	MS.gene064682:CDS	55.0%
	GTTCCGAGGGAAACGAGGTT+AGG	-	chr3.4:97082982-97083001	None:intergenic	55.0%
	TACACGTTCCGAGGGAAACG+AGG	-	chr3.4:97082987-97083006	None:intergenic	55.0%
	TGAATCGGGACCTTCGTGTG+AGG	-	chr3.4:97082869-97082888	None:intergenic	55.0%
!!	GTTGGTGGCACGCTTAACGA+GGG	-	chr3.4:97082950-97082969	None:intergenic	55.0%
!!	TGTTGGTGGCACGCTTAACG+AGG	-	chr3.4:97082951-97082970	None:intergenic	55.0%
!!!	TGTGCCTTTGGGTTTTGGGG+TGG	+	chr3.4:97083212-97083231	MS.gene064682:CDS	55.0%
	GCAGCCACCCCAAAACCCAA+AGG	-	chr3.4:97083219-97083238	None:intergenic	60.0%
	GGACGAGACAGAAACGAGCG+TGG	+	chr3.4:97082786-97082805	MS.gene064682:CDS	60.0%
	GGTGGCTGCCAAGTCTACAC+AGG	+	chr3.4:97083230-97083249	MS.gene064682:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	97082775	97083338	97082775	ID=MS.gene064682
chr3.4	mRNA	97082775	97083338	97082775	ID=MS.gene064682.t1;Parent=MS.gene064682
chr3.4	exon	97082775	97083338	97082775	ID=MS.gene064682.t1.exon1;Parent=MS.gene064682.t1
chr3.4	CDS	97082775	97083338	97082775	ID=cds.MS.gene064682.t1;Parent=MS.gene064682.t1

Gene Sequence

>MS.gene064682

ATGAGACCTTTGGACGAGACAGAAACGAGCGTGGTATTCGAAAAGCTCTTCAAATTCGTAGGAAACAATCTCAAAAACCTCGTTGAAAATCCCTCACACGAAGGTCCCGATTCAAATCCTGGTCGCTACTGTTTCCGTCTCAACAAGAACAAAATCTACTACTGCAGTGAATCCCTCGTTAAGCGTGCCACCAACATCGCTAGACCTAACCTCGTTTCCCTCGGAACGTGTATCGGAAAATACACACACGGTGGAAATTTTCATCTCACCGTTCAGGCGTTGAATTTACTTGCTGCGAATGCCAAGCATAAGGTATGGCTTAAACCACAATCTGAGATGTCTTTTTTGTATGGGAATCATGTGTTGAAGAGTGCGTTGGGTCGCATTACGGAGAATATTGTTGCTGGTGATGGGGTTGTTGTTTTTTCTATGTCGGATGTGCCTTTGGGTTTTGGGGTGGCTGCCAAGTCTACACAGGATTGTAGGAAGTTGGATCCTAATGGTATTGTTGTGCTTCATCAGGGTGATATTGGAGAGTATTTGAGGATTGAGGATGAGCTTTGA

Protein sequence

>MS.gene064682.t1

MRPLDETETSVVFEKLFKFVGNNLKNLVENPSHEGPDSNPGRYCFRLNKNKIYYCSESLVKRATNIARPNLVSLGTCIGKYTHGGNFHLTVQALNLLAANAKHKVWLKPQSEMSFLYGNHVLKSALGRITENIVAGDGVVVFSMSDVPLGFGVAAKSTQDCRKLDPNGIVVLHQGDIGEYLRIEDEL