AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059052


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059052.t1 MTR_2g035320 95.541 157 7 0 1 157 1 157 2.80e-106 300
MS.gene059052.t1 MTR_2g035190 94.268 157 9 0 1 157 1 157 8.97e-105 296
MS.gene059052.t1 MTR_2g035210 92.357 157 12 0 1 157 1 157 1.87e-103 293
MS.gene059052.t1 MTR_2g035220 89.172 157 17 0 1 157 1 157 2.99e-99 282
MS.gene059052.t1 MTR_6g033450 64.968 157 55 0 1 157 1 157 2.32e-69 207
MS.gene059052.t1 MTR_4g120970 64.968 157 55 0 1 157 1 157 2.32e-69 207
MS.gene059052.t1 MTR_2g035100 63.057 157 58 0 1 157 1 157 8.57e-67 200
MS.gene059052.t1 MTR_2g035105 62.420 157 59 0 1 157 1 157 8.64e-66 197
MS.gene059052.t1 MTR_2g035130 61.146 157 61 0 1 157 1 157 6.33e-65 196
MS.gene059052.t1 MTR_2g035150 61.146 157 61 0 1 157 1 157 2.43e-64 194
MS.gene059052.t1 MTR_2g035320 94.845 97 5 0 61 157 26 122 1.07e-61 186
MS.gene059052.t1 MTR_2g035120 55.414 157 70 0 1 157 1 157 1.82e-58 179
MS.gene059052.t1 MTR_2g035170 45.860 157 85 0 1 157 1 157 2.61e-46 148
MS.gene059052.t1 MTR_4g120950 42.236 161 86 5 1 157 1 158 8.06e-32 112
MS.gene059052.t1 MTR_2g435310 32.051 156 105 1 1 155 1 156 4.76e-30 107
MS.gene059052.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 1.18e-29 106
MS.gene059052.t1 MTR_4g120760 33.121 157 104 1 1 156 1 157 1.30e-29 106
MS.gene059052.t1 MTR_4g120940 61.644 73 28 0 85 157 47 119 1.81e-23 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACATGGATAACTTCTTAA+TGG 0.262600 2.4:+55913850 None:intergenic
GATAACTTCTTAATGGTTCC+TGG 0.335652 2.4:+55913857 None:intergenic
TACAGCTTGGTAGGAGGAAC+AGG 0.341343 2.4:-55913228 MS.gene059052:CDS
TTCAACAATTTCAACACTTT+GGG 0.350751 2.4:+55913892 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2.4:+55913891 None:intergenic
GAAACTAAGGCCAGAGGAAC+TGG 0.366866 2.4:-55913072 MS.gene059052:CDS
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2.4:+55913911 None:intergenic
AGAGCTTTGTAGAGTTTAGC+TGG 0.394931 2.4:+55913959 None:intergenic
TGCAGTAGATGAGGCAAACT+TGG 0.396721 2.4:-55913259 MS.gene059052:CDS
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2.4:-55913884 MS.gene059052:CDS
CTTGATAAGTCCAGTTCCTC+TGG 0.419380 2.4:+55913062 None:intergenic
AGATAGAGCTGCATCACCTT+TGG 0.442558 2.4:+55913110 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2.4:+55913912 None:intergenic
AAAATTGTGGCTGGCTCTGA+TGG 0.498760 2.4:-55913168 MS.gene059052:CDS
TAATTTGTGTAGCACAAAGT+TGG 0.501990 2.4:+55913284 None:intergenic
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2.4:-55913890 MS.gene059052:CDS
CAGAGGAACTGGACTTATCA+AGG 0.507213 2.4:-55913061 MS.gene059052:CDS
GGACTTATCAAGGCCATTGA+GGG 0.514208 2.4:-55913051 MS.gene059052:CDS
GGATACAACTACAGCTTGGT+AGG 0.525235 2.4:-55913237 MS.gene059052:CDS
CTCTGGCCTTAGTTTCATCA+CGG 0.532674 2.4:+55913079 None:intergenic
TGCAGTCCGTGATGAAACTA+AGG 0.533307 2.4:-55913085 MS.gene059052:CDS
TGCCACGACGTAGCCCTCAA+TGG 0.549026 2.4:+55913038 None:intergenic
TTTGACTGTAATCTTAACAA+TGG 0.571145 2.4:+55913140 None:intergenic
TGGACTTATCAAGGCCATTG+AGG 0.572485 2.4:-55913052 MS.gene059052:CDS
GTTGAAGGAAATGGAGGACC+AGG 0.585895 2.4:-55913875 MS.gene059052:CDS
CAAATTAGATGCAGTAGATG+AGG 0.587202 2.4:-55913268 MS.gene059052:CDS
ATTGTGGCTGGCTCTGATGG+TGG 0.588345 2.4:-55913165 MS.gene059052:CDS
GCAGTAGATGAGGCAAACTT+GGG 0.598362 2.4:-55913258 MS.gene059052:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2.4:+55913967 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2.4:-55913927 MS.gene059052:CDS
CTTGGTAGGAGGAACAGGGT+TGG 0.609437 2.4:-55913223 MS.gene059052:CDS
CTTGGGATACAACTACAGCT+TGG 0.614144 2.4:-55913241 MS.gene059052:CDS
AAGAAGTTATCCATGTCTGA+AGG 0.619942 2.4:-55913845 MS.gene059052:intron
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2.4:-55913881 MS.gene059052:CDS
TACAACTACAGCTTGGTAGG+AGG 0.632731 2.4:-55913234 MS.gene059052:CDS
GGCCATTGAGGGCTACGTCG+TGG 0.646344 2.4:-55913040 MS.gene059052:CDS
TCTTAAATTACCTTCAGACA+TGG 0.649758 2.4:+55913835 None:intergenic
ACAGTCAAATACCACACCAA+AGG 0.652327 2.4:-55913126 MS.gene059052:CDS
GAGCTGCATCACCTTTGGTG+TGG 0.654393 2.4:+55913115 None:intergenic
CGTGATGAAACTAAGGCCAG+AGG 0.688505 2.4:-55913078 MS.gene059052:CDS
ACAGCTTGGTAGGAGGAACA+GGG 0.710550 2.4:-55913227 MS.gene059052:CDS
TGATGAACATGTATCAACCG+TGG 0.718349 2.4:-55913984 MS.gene059052:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATAAATAAATTATAA+TGG - chr2.4:55913700-55913719 MS.gene059052:intron 0.0%
!!! AAATAAAAGTATAACATAAA+TGG + chr2.4:55913504-55913523 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAGTAAA+AGG + chr2.4:55913338-55913357 None:intergenic 15.0%
!!! CACATGAATCATTTTTTTTA+AGG + chr2.4:55913600-55913619 None:intergenic 20.0%
!!! GAATTTAACTTACTTTTCTT+TGG + chr2.4:55913428-55913447 None:intergenic 20.0%
! AATAGAATCTTGAGAAATGA+AGG - chr2.4:55913528-55913547 MS.gene059052:intron 25.0%
! ATAATTATTAGACATACTCC+TGG + chr2.4:55913574-55913593 None:intergenic 25.0%
! TGAATTCGAAACAAAAATTG+TGG - chr2.4:55913846-55913865 MS.gene059052:CDS 25.0%
! TTCAACAATTTCAACACTTT+GGG + chr2.4:55913138-55913157 None:intergenic 25.0%
! TTTGACTGTAATCTTAACAA+TGG + chr2.4:55913890-55913909 None:intergenic 25.0%
!! AGGAGTATGTCTAATAATTA+TGG - chr2.4:55913573-55913592 MS.gene059052:intron 25.0%
AGACATGGATAACTTCTTAA+TGG + chr2.4:55913180-55913199 None:intergenic 30.0%
CTTCAACAATTTCAACACTT+TGG + chr2.4:55913139-55913158 None:intergenic 30.0%
GTTGAAATTGTTGAAGGAAA+TGG - chr2.4:55913143-55913162 MS.gene059052:CDS 30.0%
TCTTAAATTACCTTCAGACA+TGG + chr2.4:55913195-55913214 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG + chr2.4:55913746-55913765 None:intergenic 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG - chr2.4:55913137-55913156 MS.gene059052:CDS 30.0%
!! TGTAAAATTGTTTTACACCG+AGG - chr2.4:55913270-55913289 MS.gene059052:CDS 30.0%
AAGAAGTTATCCATGTCTGA+AGG - chr2.4:55913182-55913201 MS.gene059052:CDS 35.0%
ATAATACATAAGCTCGTTCC+AGG - chr2.4:55913553-55913572 MS.gene059052:intron 35.0%
CAAATTAGATGCAGTAGATG+AGG - chr2.4:55913759-55913778 MS.gene059052:intron 35.0%
GAAATTGTTGAAGGAAATGG+AGG - chr2.4:55913146-55913165 MS.gene059052:CDS 35.0%
GATAACTTCTTAATGGTTCC+TGG + chr2.4:55913173-55913192 None:intergenic 35.0%
TATAATGGCTGTATTGCAGA+TGG - chr2.4:55913715-55913734 MS.gene059052:intron 35.0%
TTCGAAACAAAAATTGTGGC+TGG - chr2.4:55913850-55913869 MS.gene059052:CDS 35.0%
! TACACAATTCTTTGTTGTGC+TGG + chr2.4:55913311-55913330 None:intergenic 35.0%
ACAGTCAAATACCACACCAA+AGG - chr2.4:55913901-55913920 MS.gene059052:CDS 40.0%
CGTGACATATTGAAGAGTTG+TGG - chr2.4:55913363-55913382 MS.gene059052:intron 40.0%
TGATGAACATGTATCAACCG+TGG - chr2.4:55913043-55913062 MS.gene059052:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG + chr2.4:55913071-55913090 None:intergenic 40.0%
AGATAGAGCTGCATCACCTT+TGG + chr2.4:55913920-55913939 None:intergenic 45.0%
CAGAGGAACTGGACTTATCA+AGG - chr2.4:55913966-55913985 MS.gene059052:CDS 45.0%
CTTGATAAGTCCAGTTCCTC+TGG + chr2.4:55913968-55913987 None:intergenic 45.0%
CTTGGGATACAACTACAGCT+TGG - chr2.4:55913786-55913805 MS.gene059052:intron 45.0%
GCAGTAGATGAGGCAAACTT+GGG - chr2.4:55913769-55913788 MS.gene059052:intron 45.0%
GGACTTATCAAGGCCATTGA+GGG - chr2.4:55913976-55913995 MS.gene059052:CDS 45.0%
GGATACAACTACAGCTTGGT+AGG - chr2.4:55913790-55913809 MS.gene059052:intron 45.0%
TACAACTACAGCTTGGTAGG+AGG - chr2.4:55913793-55913812 MS.gene059052:intron 45.0%
TGCAGTAGATGAGGCAAACT+TGG - chr2.4:55913768-55913787 MS.gene059052:intron 45.0%
TGCAGTCCGTGATGAAACTA+AGG - chr2.4:55913942-55913961 MS.gene059052:CDS 45.0%
TGCTGATGAAATCGTCCCAA+AGG - chr2.4:55913100-55913119 MS.gene059052:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG - chr2.4:55913975-55913994 MS.gene059052:CDS 45.0%
!! AAAATTGTGGCTGGCTCTGA+TGG - chr2.4:55913859-55913878 MS.gene059052:CDS 45.0%
!! CTCTGGCCTTAGTTTCATCA+CGG + chr2.4:55913951-55913970 None:intergenic 45.0%
!! GGCGAATTTTGATCGATCCT+CGG + chr2.4:55913290-55913309 None:intergenic 45.0%
ACAGCTTGGTAGGAGGAACA+GGG - chr2.4:55913800-55913819 MS.gene059052:intron 50.0%
CGTGATGAAACTAAGGCCAG+AGG - chr2.4:55913949-55913968 MS.gene059052:CDS 50.0%
GAAACTAAGGCCAGAGGAAC+TGG - chr2.4:55913955-55913974 MS.gene059052:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG + chr2.4:55913118-55913137 None:intergenic 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG + chr2.4:55913063-55913082 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+AGG - chr2.4:55913152-55913171 MS.gene059052:CDS 50.0%
TACAGCTTGGTAGGAGGAAC+AGG - chr2.4:55913799-55913818 MS.gene059052:intron 50.0%
TGGGCAGCAGAAATCACCTT+TGG + chr2.4:55913119-55913138 None:intergenic 50.0%
CTTGGTAGGAGGAACAGGGT+TGG - chr2.4:55913804-55913823 MS.gene059052:intron 55.0%
GAGCTGCATCACCTTTGGTG+TGG + chr2.4:55913915-55913934 None:intergenic 55.0%
!! ATTGTGGCTGGCTCTGATGG+TGG - chr2.4:55913862-55913881 MS.gene059052:CDS 55.0%
TGCCACGACGTAGCCCTCAA+TGG + chr2.4:55913992-55914011 None:intergenic 60.0%
GGCCATTGAGGGCTACGTCG+TGG - chr2.4:55913987-55914006 MS.gene059052:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 55913023 55914026 55913023 ID=MS.gene059052
chr2.4 mRNA 55913023 55914026 55913023 ID=MS.gene059052.t1;Parent=MS.gene059052
chr2.4 exon 55913846 55914026 55913846 ID=MS.gene059052.t1.exon1;Parent=MS.gene059052.t1
chr2.4 CDS 55913846 55914026 55913846 ID=cds.MS.gene059052.t1;Parent=MS.gene059052.t1
chr2.4 exon 55913023 55913315 55913023 ID=MS.gene059052.t1.exon2;Parent=MS.gene059052.t1
chr2.4 CDS 55913023 55913315 55913023 ID=cds.MS.gene059052.t1;Parent=MS.gene059052.t1
Gene Sequence

>MS.gene059052

ATGGGTGTTTTTACTTTCAATGATGAACATGTATCAACCGTGGCTCCAGCTAAACTCTACAAAGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCAGGAACCATTAAGAAGTTATCCATGTCTGAAGGTAATTTAAGAGCATATAGTATGTACAAATATATAATACTTGAGATCAAATGATACATAGTGTTCGTGTAAAATTGTTTTACACCGAGGATCGATCAAAATTCGCCAGCACAACAAAGAATTGTGTAACTACCTTTTACTTAAAAATTGACAAATATGACGTGACATATTGAAGAGTTGTGGATTAAATGATTCACAGATAAGTAAGTTAAATTCATTATACCAAAGAAAAGTAAGTTAAATTCATTACTACTAAAAAAAAAAAGTAAGTTAAATTTAAGTGTAGAAAAAATAGAAGCCATTTATGTTATACTTTTATTTTTAAAATAGAATCTTGAGAAATGAAGGAGATAATACATAAGCTCGTTCCAGGAGTATGTCTAATAATTATGGACCTTAAAAAAAATGATTCATGTGATTAATGATACTCGATAAAGTTCAAATCGATCTTTAAACGAAAGAGTTTTTAGTCAAACTTTACTTATATCGAAAAAAGAATTATATAAATAAATTATAATGGCTGTATTGCAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATGCAGTAGATGAGGCAAACTTGGGATACAACTACAGCTTGGTAGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTCGAAACAAAAATTGTGGCTGGCTCTGATGGTGGATCCATTGTTAAGATTACAGTCAAATACCACACCAAAGGTGATGCAGCTCTATCTGATGCAGTCCGTGATGAAACTAAGGCCAGAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTCGTGGCAAACCCTAATTACTAA

Protein sequence

>MS.gene059052.t1

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSMSEDGKTNFVLHKLDAVDEANLGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIVKITVKYHTKGDAALSDAVRDETKARGTGLIKAIEGYVVANPNY