AlfalfaGEDB | MS.gene45921

M. sativa cultivar XinJiangDaYe / MS.gene45921

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45921.t1	PNY11092.1	82.5	257	45	0	3	259	2	258	2.50E-108	401.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45921.t1	A0A2K3P729	82.5	257	45	0	3	259	2	258	1.8e-108	401.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene45921	0.801748	7.78E-49	-1.69E-46
MS.gene049346	MS.gene45921	0.852756	3.75E-61	-1.69E-46
MS.gene049662	MS.gene45921	0.802964	4.37E-49	-1.69E-46
MS.gene049758	MS.gene45921	0.825004	5.94E-54	-1.69E-46
MS.gene049910	MS.gene45921	0.818242	2.17E-52	-1.69E-46
MS.gene050301	MS.gene45921	0.847697	9.82E-60	-1.69E-46
MS.gene050330	MS.gene45921	-0.803999	2.67E-49	-1.69E-46
MS.gene050397	MS.gene45921	0.826455	2.69E-54	-1.69E-46
MS.gene050406	MS.gene45921	0.81956	1.09E-52	-1.69E-46
MS.gene050426	MS.gene45921	-0.815857	7.46E-52	-1.69E-46
MS.gene050464	MS.gene45921	0.815352	9.66E-52	-1.69E-46
MS.gene050529	MS.gene45921	0.807608	4.65E-50	-1.69E-46
MS.gene050558	MS.gene45921	0.821921	3.12E-53	-1.69E-46
MS.gene050642	MS.gene45921	0.82411	9.64E-54	-1.69E-46
MS.gene050670	MS.gene45921	0.814862	1.24E-51	-1.69E-46
MS.gene05101	MS.gene45921	0.810117	1.35E-50	-1.69E-46
MS.gene051525	MS.gene45921	0.811668	6.24E-51	-1.69E-46
MS.gene051582	MS.gene45921	0.863939	1.74E-64	-1.69E-46
MS.gene051860	MS.gene45921	0.820113	8.14E-53	-1.69E-46
MS.gene051983	MS.gene45921	0.808893	2.48E-50	-1.69E-46
MS.gene052011	MS.gene45921	-0.848289	6.74E-60	-1.69E-46
MS.gene052077	MS.gene45921	0.814239	1.70E-51	-1.69E-46
MS.gene052188	MS.gene45921	0.809518	1.82E-50	-1.69E-46
MS.gene052253	MS.gene45921	0.845984	2.89E-59	-1.69E-46
MS.gene052310	MS.gene45921	0.828493	8.72E-55	-1.69E-46
MS.gene052340	MS.gene45921	0.840239	9.80E-58	-1.69E-46
MS.gene052481	MS.gene45921	0.816365	5.74E-52	-1.69E-46
MS.gene052485	MS.gene45921	0.811937	5.45E-51	-1.69E-46
MS.gene053330	MS.gene45921	0.84119	5.52E-58	-1.69E-46
MS.gene053836	MS.gene45921	0.863769	1.96E-64	-1.69E-46
MS.gene053971	MS.gene45921	0.830451	2.92E-55	-1.69E-46
MS.gene054183	MS.gene45921	0.825087	5.68E-54	-1.69E-46
MS.gene054473	MS.gene45921	0.843054	1.77E-58	-1.69E-46
MS.gene054719	MS.gene45921	0.801723	7.87E-49	-1.69E-46
MS.gene054930	MS.gene45921	0.801525	8.64E-49	-1.69E-46
MS.gene054983	MS.gene45921	0.885249	9.57E-72	-1.69E-46
MS.gene055116	MS.gene45921	0.838046	3.63E-57	-1.69E-46
MS.gene055277	MS.gene45921	0.816156	6.39E-52	-1.69E-46
MS.gene055422	MS.gene45921	0.826036	3.38E-54	-1.69E-46
MS.gene055428	MS.gene45921	0.859873	3.06E-63	-1.69E-46
MS.gene05553	MS.gene45921	0.845401	4.16E-59	-1.69E-46
MS.gene056407	MS.gene45921	0.807401	5.15E-50	-1.69E-46
MS.gene056451	MS.gene45921	0.814777	1.30E-51	-1.69E-46
MS.gene056612	MS.gene45921	0.818637	1.77E-52	-1.69E-46
MS.gene056723	MS.gene45921	0.836579	8.62E-57	-1.69E-46
MS.gene056899	MS.gene45921	-0.831019	2.12E-55	-1.69E-46
MS.gene05702	MS.gene45921	0.862332	5.46E-64	-1.69E-46
MS.gene057121	MS.gene45921	0.847442	1.15E-59	-1.69E-46
MS.gene057245	MS.gene45921	0.825574	4.35E-54	-1.69E-46
MS.gene05733	MS.gene45921	0.811348	7.32E-51	-1.69E-46
MS.gene057749	MS.gene45921	0.807886	4.06E-50	-1.69E-46
MS.gene058002	MS.gene45921	0.870482	1.41E-66	-1.69E-46
MS.gene05811	MS.gene45921	0.846986	1.54E-59	-1.69E-46
MS.gene058142	MS.gene45921	0.832656	8.35E-56	-1.69E-46
MS.gene058233	MS.gene45921	0.851647	7.74E-61	-1.69E-46
MS.gene058276	MS.gene45921	0.839921	1.19E-57	-1.69E-46
MS.gene058660	MS.gene45921	0.810407	1.17E-50	-1.69E-46
MS.gene058710	MS.gene45921	0.810896	9.18E-51	-1.69E-46
MS.gene058746	MS.gene45921	0.813793	2.14E-51	-1.69E-46
MS.gene05886	MS.gene45921	0.810586	1.07E-50	-1.69E-46
MS.gene059011	MS.gene45921	0.810694	1.01E-50	-1.69E-46
MS.gene05907	MS.gene45921	0.845955	2.94E-59	-1.69E-46
MS.gene059141	MS.gene45921	0.825778	3.89E-54	-1.69E-46
MS.gene059247	MS.gene45921	0.822955	1.80E-53	-1.69E-46
MS.gene059285	MS.gene45921	0.80356	3.29E-49	-1.69E-46
MS.gene059420	MS.gene45921	0.840648	7.66E-58	-1.69E-46
MS.gene059518	MS.gene45921	0.802361	5.82E-49	-1.69E-46
MS.gene059597	MS.gene45921	0.810557	1.09E-50	-1.69E-46
MS.gene059688	MS.gene45921	0.825454	4.65E-54	-1.69E-46
MS.gene059692	MS.gene45921	0.827474	1.53E-54	-1.69E-46
MS.gene059695	MS.gene45921	0.827043	1.95E-54	-1.69E-46
MS.gene05984	MS.gene45921	0.859845	3.12E-63	-1.69E-46
MS.gene059894	MS.gene45921	0.838248	3.22E-57	-1.69E-46
MS.gene060403	MS.gene45921	0.815851	7.48E-52	-1.69E-46
MS.gene060407	MS.gene45921	0.823765	1.16E-53	-1.69E-46
MS.gene061049	MS.gene45921	0.865213	6.95E-65	-1.69E-46
MS.gene061114	MS.gene45921	-0.80558	1.25E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45921.t1	MTR_7g067000	95.331	257	12	0	3	259	1	257	3.15e-176	485
MS.gene45921.t1	MTR_4g015010	57.708	253	94	5	2	241	13	265	3.92e-90	268

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45921.t1	AT3G09050	59.896	192	75	2	51	242	56	245	4.11e-76	232

Find 39 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCATTGTTGGTCTTTCTTA+TGG	0.167344	7.2:+45618873	None:intergenic
TCTGCATACTTAACTCAATT+TGG	0.207885	7.2:-45619459	MS.gene45921:CDS
GAGTTTGAAGCGTGACAATT+TGG	0.294321	7.2:+45619517	None:intergenic
AATGTTTACCTCTTCTTCAT+TGG	0.306206	7.2:-45619033	MS.gene45921:CDS
CTTGATGCTGTTCTGTTTGA+AGG	0.380275	7.2:+45619662	None:intergenic
TTGCGTCGCCTTCGACTGAT+TGG	0.388513	7.2:-45618981	MS.gene45921:CDS
TAAAGGCTGGTAGTGGAATC+AGG	0.392256	7.2:-45619059	MS.gene45921:CDS
AACGCCGAATTTGAAAAGAT+TGG	0.418571	7.2:-45619426	MS.gene45921:CDS
CTGTTTGAAGGAGGGATGTA+GGG	0.419954	7.2:+45619674	None:intergenic
AGCAGAGTTTGAAGTTCAAA+TGG	0.422016	7.2:-45619193	MS.gene45921:CDS
TCCACTACCAGCCTTTAGTC+TGG	0.433418	7.2:+45619065	None:intergenic
AGTTTGAAGCGTGACAATTT+GGG	0.437074	7.2:+45619518	None:intergenic
TGTGCCTCTGCAAGTCCAAT+GGG	0.448799	7.2:-45619593	MS.gene45921:CDS
TTGTGCCTCTGCAAGTCCAA+TGG	0.464033	7.2:-45619594	MS.gene45921:CDS
TCTGTTTGAAGGAGGGATGT+AGG	0.485064	7.2:+45619673	None:intergenic
CAACAGGTGCCTTCTTTCTG+AGG	0.485279	7.2:+45619136	None:intergenic
TTTGAAGTTCAAATGGAAAA+CGG	0.485912	7.2:-45619186	MS.gene45921:CDS
CTTTAAGAACCTCAGAAAGA+AGG	0.489538	7.2:-45619145	MS.gene45921:CDS
ACCAGACTAAAGGCTGGTAG+TGG	0.519451	7.2:-45619066	MS.gene45921:CDS
ATGCTGTTCTGTTTGAAGGA+GGG	0.519954	7.2:+45619666	None:intergenic
TAAAGATGTAACCAGACTAA+AGG	0.520928	7.2:-45619076	MS.gene45921:CDS
AAACTACTGTAAAGGTCAGC+AGG	0.526715	7.2:+45619010	None:intergenic
ATCTGCAGAAATTAACAGAC+AGG	0.528683	7.2:-45619241	MS.gene45921:CDS
AGCTTGAGAATCAGACCCAT+TGG	0.534125	7.2:+45619578	None:intergenic
AGCAAAAGCCAAAGAGGAGG+CGG	0.536159	7.2:-45619106	MS.gene45921:CDS
AATAGAATAAACTACTGTAA+AGG	0.541708	7.2:+45619002	None:intergenic
GATTCTCAAGCTCCTCAACC+AGG	0.562806	7.2:-45619567	MS.gene45921:CDS
GAGCCAGATGCTAAAATTCG+AGG	0.565238	7.2:-45619265	MS.gene45921:CDS
ATGGAAAACGGCAGAAACGA+AGG	0.568917	7.2:-45619174	MS.gene45921:CDS
TTAACAGATAACAGCGAACA+TGG	0.573660	7.2:-45619289	MS.gene45921:intron
TCAGCAGGCCAATGAAGAAG+AGG	0.579281	7.2:+45619025	None:intergenic
GATGCTGTTCTGTTTGAAGG+AGG	0.585163	7.2:+45619665	None:intergenic
GATGTAACCAGACTAAAGGC+TGG	0.590246	7.2:-45619072	MS.gene45921:CDS
GTATAACTTCTTGTGTTGTG+AGG	0.614593	7.2:+45619618	None:intergenic
TGATAAAGCAAAAGCCAAAG+AGG	0.625476	7.2:-45619112	MS.gene45921:CDS
TGGAAAACGGCAGAAACGAA+GGG	0.629953	7.2:-45619173	MS.gene45921:CDS
CATCTAGATACTCTGTAAGA+CGG	0.659918	7.2:+45619487	None:intergenic
CAGACCCATTGGACTTGCAG+AGG	0.674115	7.2:+45619589	None:intergenic
TAAAGCAAAAGCCAAAGAGG+AGG	0.690698	7.2:-45619109	MS.gene45921:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATACAAAATGAATATAAG+AGG	+	chr7.2:45619247-45619266	None:intergenic	15.0%
!!!	TTTTTTATTTACATTTGACA+TGG	+	chr7.2:45619221-45619240	None:intergenic	15.0%
!!	AATAGAATAAACTACTGTAA+AGG	+	chr7.2:45619555-45619574	None:intergenic	20.0%
!!	TTCTCCAATCTTTTCAAATT+CGG	+	chr7.2:45619135-45619154	None:intergenic	25.0%
!!	TTTGAAGTTCAAATGGAAAA+CGG	-	chr7.2:45619368-45619387	MS.gene45921:intron	25.0%
!!!	TTTGTTTTCTTCTTCATTGT+TGG	+	chr7.2:45619696-45619715	None:intergenic	25.0%
	AATGTTTACCTCTTCTTCAT+TGG	-	chr7.2:45619521-45619540	MS.gene45921:CDS	30.0%
	TAAAGATGTAACCAGACTAA+AGG	-	chr7.2:45619478-45619497	MS.gene45921:CDS	30.0%
	TCTGCATACTTAACTCAATT+TGG	-	chr7.2:45619095-45619114	MS.gene45921:CDS	30.0%
!!	TTCATTGTTGGTCTTTCTTA+TGG	+	chr7.2:45619684-45619703	None:intergenic	30.0%
!!!	TCTTGTTTTTTTGTATCTCC+TGG	+	chr7.2:45619008-45619027	None:intergenic	30.0%
	AACGCCGAATTTGAAAAGAT+TGG	-	chr7.2:45619128-45619147	MS.gene45921:CDS	35.0%
	ATCTGCAGAAATTAACAGAC+AGG	-	chr7.2:45619313-45619332	MS.gene45921:intron	35.0%
	CATCTAGATACTCTGTAAGA+CGG	+	chr7.2:45619070-45619089	None:intergenic	35.0%
	GTATAACTTCTTGTGTTGTG+AGG	+	chr7.2:45618939-45618958	None:intergenic	35.0%
	TGATAAAGCAAAAGCCAAAG+AGG	-	chr7.2:45619442-45619461	MS.gene45921:CDS	35.0%
	TTAACAGATAACAGCGAACA+TGG	-	chr7.2:45619265-45619284	MS.gene45921:CDS	35.0%
!	AGCAGAGTTTGAAGTTCAAA+TGG	-	chr7.2:45619361-45619380	MS.gene45921:intron	35.0%
!	AGTTTGAAGCGTGACAATTT+GGG	+	chr7.2:45619039-45619058	None:intergenic	35.0%
!	CTTTAAGAACCTCAGAAAGA+AGG	-	chr7.2:45619409-45619428	MS.gene45921:CDS	35.0%
!	GCTTTTGCTTTATCAACAAC+AGG	+	chr7.2:45619437-45619456	None:intergenic	35.0%
!	TGGAGAAAAGTTGCAGTTTT+TGG	-	chr7.2:45619593-45619612	MS.gene45921:CDS	35.0%
!!	ATTCCTCGAATTTTAGCATC+TGG	+	chr7.2:45619295-45619314	None:intergenic	35.0%
!!!	AGTTTTTGGAGCAATTCTTG+TGG	-	chr7.2:45619607-45619626	MS.gene45921:CDS	35.0%
!!!	TTTTTTGTATCTCCTGGTTG+AGG	+	chr7.2:45619002-45619021	None:intergenic	35.0%
	AAACTACTGTAAAGGTCAGC+AGG	+	chr7.2:45619547-45619566	None:intergenic	40.0%
	ATGCTGTTCTGTTTGAAGGA+GGG	+	chr7.2:45618891-45618910	None:intergenic	40.0%
	CTTGATGCTGTTCTGTTTGA+AGG	+	chr7.2:45618895-45618914	None:intergenic	40.0%
	TAAAGCAAAAGCCAAAGAGG+AGG	-	chr7.2:45619445-45619464	MS.gene45921:CDS	40.0%
!	ACTTTTCTCCAATCAGTCGA+AGG	+	chr7.2:45619584-45619603	None:intergenic	40.0%
!	GAGTTTGAAGCGTGACAATT+TGG	+	chr7.2:45619040-45619059	None:intergenic	40.0%
!!!	AATTTTTTCCGCCTCCTCTT+TGG	+	chr7.2:45619459-45619478	None:intergenic	40.0%
	AAGTGGAAGCAAGCATACTC+TGG	+	chr7.2:45619189-45619208	None:intergenic	45.0%
	AGCTTGAGAATCAGACCCAT+TGG	+	chr7.2:45618979-45618998	None:intergenic	45.0%
	ATGGAAAACGGCAGAAACGA+AGG	-	chr7.2:45619380-45619399	MS.gene45921:intron	45.0%
	CTGTTTGAAGGAGGGATGTA+GGG	+	chr7.2:45618883-45618902	None:intergenic	45.0%
	GAGCCAGATGCTAAAATTCG+AGG	-	chr7.2:45619289-45619308	MS.gene45921:intron	45.0%
	GATGCTGTTCTGTTTGAAGG+AGG	+	chr7.2:45618892-45618911	None:intergenic	45.0%
	GATGTAACCAGACTAAAGGC+TGG	-	chr7.2:45619482-45619501	MS.gene45921:CDS	45.0%
	TCTGTTTGAAGGAGGGATGT+AGG	+	chr7.2:45618884-45618903	None:intergenic	45.0%
	TGACATGGATAGCTCGAAAG+TGG	+	chr7.2:45619206-45619225	None:intergenic	45.0%
	TGGAAAACGGCAGAAACGAA+GGG	-	chr7.2:45619381-45619400	MS.gene45921:intron	45.0%
!	TGTTGTGAGGTCTTTTGCAG+AGG	+	chr7.2:45618926-45618945	None:intergenic	45.0%
!!	TAAAGGCTGGTAGTGGAATC+AGG	-	chr7.2:45619495-45619514	MS.gene45921:CDS	45.0%
	ACCAGACTAAAGGCTGGTAG+TGG	-	chr7.2:45619488-45619507	MS.gene45921:CDS	50.0%
	AGCAAAAGCCAAAGAGGAGG+CGG	-	chr7.2:45619448-45619467	MS.gene45921:CDS	50.0%
	CAACAGGTGCCTTCTTTCTG+AGG	+	chr7.2:45619421-45619440	None:intergenic	50.0%
	GATTCTCAAGCTCCTCAACC+AGG	-	chr7.2:45618987-45619006	MS.gene45921:CDS	50.0%
	TCAGCAGGCCAATGAAGAAG+AGG	+	chr7.2:45619532-45619551	None:intergenic	50.0%
	TCCACTACCAGCCTTTAGTC+TGG	+	chr7.2:45619492-45619511	None:intergenic	50.0%
	TGTGCCTCTGCAAGTCCAAT+GGG	-	chr7.2:45618961-45618980	MS.gene45921:CDS	50.0%
	TTGTGCCTCTGCAAGTCCAA+TGG	-	chr7.2:45618960-45618979	MS.gene45921:CDS	50.0%
	CAGACCCATTGGACTTGCAG+AGG	+	chr7.2:45618968-45618987	None:intergenic	55.0%
	TTGCGTCGCCTTCGACTGAT+TGG	-	chr7.2:45619573-45619592	MS.gene45921:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	45618858	45619718	45618858	ID=MS.gene45921
chr7.2	mRNA	45618858	45619718	45618858	ID=MS.gene45921.t1;Parent=MS.gene45921
chr7.2	exon	45619386	45619718	45619386	ID=MS.gene45921.t1.exon1;Parent=MS.gene45921.t1
chr7.2	CDS	45619386	45619718	45619386	ID=cds.MS.gene45921.t1;Parent=MS.gene45921.t1
chr7.2	exon	45618858	45619304	45618858	ID=MS.gene45921.t1.exon2;Parent=MS.gene45921.t1
chr7.2	CDS	45618858	45619304	45618858	ID=cds.MS.gene45921.t1;Parent=MS.gene45921.t1

Gene Sequence

>MS.gene45921

ATGAAGATGATTGCTCTCAAATCCCTACATCCCTCCTTCAAACAGAACAGCATCAAGTGTAATATCCTCTGCAAAAGACCTCACAACACAAGAAGTTATACTTTGTGCCTCTGCAAGTCCAATGGGTCTGATTCTCAAGCTCCTCAACCAGGAGATACAAAAAAACAAGAACTTCTAGCCCAAATTGTCACGCTTCAAACTCGAAAAGTCCGTCTTACAGAGTATCTAGATGAGAGATCTGCATACTTAACTCAATTTGGTGAAAAAGTAAACGCCGAATTTGAAAAGATTGGAGAAGATGCACTCAAAGAATTAGATGAAGCTAGTGCCAGAGTATGCTTGCTTCCACTTTCGAGCTATCCATGTCAAATGTAAATAAAAAATGACCTCTTATATTCATTTTGTATTTAACAGATAACAGCGAACATGGAGAGCCAGATGCTAAAATTCGAGGAATCTGCAGAAATTAACAGACAGGAGATTCAAGAGCGTGAAAACGAATTAGCAGAGTTTGAAGTTCAAATGGAAAACGGCAGAAACGAAGGGCTATTCTTTAAGAACCTCAGAAAGAAGGCACCTGTTGTTGATAAAGCAAAAGCCAAAGAGGAGGCGGAAAAAATTAAAGATGTAACCAGACTAAAGGCTGGTAGTGGAATCAGGAAAAATGTTTACCTCTTCTTCATTGGCCTGCTGACCTTTACAGTAGTTTATTCTATTGCGTCGCCTTCGACTGATTGGAGAAAAGTTGCAGTTTTTGGAGCAATTCTTGTGGCTTTGACTTCTCAGTTCGTCTATGAACAAAACATGTCAGCAGACACTAGAACCATAAGAAAGACCAACAATGAAGAAGAAAACAAATGA

Protein sequence

>MS.gene45921.t1

MKMIALKSLHPSFKQNSIKCNILCKRPHNTRSYTLCLCKSNGSDSQAPQPGDTKKQELLAQIVTLQTRKVRLTEYLDERSAYLTQFGEKVNAEFEKIGEDALKELDEASARITANMESQMLKFEESAEINRQEIQERENELAEFEVQMENGRNEGLFFKNLRKKAPVVDKAKAKEEAEKIKDVTRLKAGSGIRKNVYLFFIGLLTFTVVYSIASPSTDWRKVAVFGAILVALTSQFVYEQNMSADTRTIRKTNNEEENK