Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51658.t1 | XP_003598314.2 | 94.9 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 2.40E-70 | 274.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51658.t1 | A6QLK2 | 36.1 | 133 | 79 | 3 | 4 | 135 | 7 | 134 | 2.1e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51658.t1 | G7J0F9 | 94.9 | 137 | 7 | 0 | 1 | 137 | 60 | 196 | 1.8e-70 | 274.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049756 | MS.gene51658 | 0.816763 | 4.68E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene51658 | 0.859118 | 5.16E-63 | -1.69E-46 |
| MS.gene050317 | MS.gene51658 | 0.822861 | 1.89E-53 | -1.69E-46 |
| MS.gene050320 | MS.gene51658 | 0.836466 | 9.20E-57 | -1.69E-46 |
| MS.gene051063 | MS.gene51658 | 0.836981 | 6.80E-57 | -1.69E-46 |
| MS.gene051245 | MS.gene51658 | 0.865134 | 7.36E-65 | -1.69E-46 |
| MS.gene051451 | MS.gene51658 | 0.82266 | 2.10E-53 | -1.69E-46 |
| MS.gene051633 | MS.gene51658 | 0.800988 | 1.11E-48 | -1.69E-46 |
| MS.gene051746 | MS.gene51658 | 0.842278 | 2.85E-58 | -1.69E-46 |
| MS.gene051748 | MS.gene51658 | 0.844542 | 7.09E-59 | -1.69E-46 |
| MS.gene051756 | MS.gene51658 | 0.847916 | 8.54E-60 | -1.69E-46 |
| MS.gene051757 | MS.gene51658 | 0.845574 | 3.73E-59 | -1.69E-46 |
| MS.gene051903 | MS.gene51658 | 0.862479 | 4.92E-64 | -1.69E-46 |
| MS.gene052202 | MS.gene51658 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene51658 | 0.816572 | 5.16E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene51658 | 0.826534 | 2.57E-54 | -1.69E-46 |
| MS.gene052376 | MS.gene51658 | 0.8206 | 6.30E-53 | -1.69E-46 |
| MS.gene052947 | MS.gene51658 | 0.807111 | 5.93E-50 | -1.69E-46 |
| MS.gene053109 | MS.gene51658 | 0.84526 | 4.54E-59 | -1.69E-46 |
| MS.gene055174 | MS.gene51658 | 0.831975 | 1.23E-55 | -1.69E-46 |
| MS.gene055424 | MS.gene51658 | 0.802917 | 4.47E-49 | -1.69E-46 |
| MS.gene055441 | MS.gene51658 | 0.830947 | 2.20E-55 | -1.69E-46 |
| MS.gene055591 | MS.gene51658 | -0.818644 | 1.76E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene51658 | 0.818983 | 1.47E-52 | -1.69E-46 |
| MS.gene056580 | MS.gene51658 | 0.805744 | 1.15E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene51658 | 0.827123 | 1.86E-54 | -1.69E-46 |
| MS.gene057057 | MS.gene51658 | 0.833596 | 4.87E-56 | -1.69E-46 |
| MS.gene057094 | MS.gene51658 | 0.862583 | 4.57E-64 | -1.69E-46 |
| MS.gene057140 | MS.gene51658 | 0.813782 | 2.15E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene51658 | 0.883386 | 4.69E-71 | -1.69E-46 |
| MS.gene057999 | MS.gene51658 | 0.816982 | 4.18E-52 | -1.69E-46 |
| MS.gene058054 | MS.gene51658 | 0.805839 | 1.10E-49 | -1.69E-46 |
| MS.gene058055 | MS.gene51658 | 0.848507 | 5.86E-60 | -1.69E-46 |
| MS.gene058074 | MS.gene51658 | 0.800848 | 1.19E-48 | -1.69E-46 |
| MS.gene058182 | MS.gene51658 | 0.838758 | 2.38E-57 | -1.69E-46 |
| MS.gene058238 | MS.gene51658 | 0.866287 | 3.18E-65 | -1.69E-46 |
| MS.gene058906 | MS.gene51658 | 0.864284 | 1.36E-64 | -1.69E-46 |
| MS.gene059171 | MS.gene51658 | 0.802672 | 5.02E-49 | -1.69E-46 |
| MS.gene059898 | MS.gene51658 | 0.840264 | 9.66E-58 | -1.69E-46 |
| MS.gene06001 | MS.gene51658 | 0.861089 | 1.31E-63 | -1.69E-46 |
| MS.gene060297 | MS.gene51658 | 0.802792 | 4.74E-49 | -1.69E-46 |
| MS.gene060463 | MS.gene51658 | 0.803071 | 4.15E-49 | -1.69E-46 |
| MS.gene060465 | MS.gene51658 | 0.805504 | 1.29E-49 | -1.69E-46 |
| MS.gene060586 | MS.gene51658 | 0.800239 | 1.58E-48 | -1.69E-46 |
| MS.gene060702 | MS.gene51658 | 0.827703 | 1.35E-54 | -1.69E-46 |
| MS.gene060869 | MS.gene51658 | 0.810346 | 1.21E-50 | -1.69E-46 |
| MS.gene061145 | MS.gene51658 | 0.811295 | 7.52E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51658.t1 | MTR_3g010210 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 2.49e-98 | 278 |
| MS.gene51658.t1 | MTR_3g010210 | 94.891 | 137 | 7 | 0 | 1 | 137 | 60 | 196 | 1.69e-97 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51658.t1 | AT5G63690 | 66.176 | 136 | 45 | 1 | 2 | 136 | 3 | 138 | 5.34e-67 | 199 |
| MS.gene51658.t1 | AT5G63690 | 66.176 | 136 | 45 | 1 | 2 | 136 | 3 | 138 | 5.34e-67 | 199 |
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACACTAAATTTATAATTT+TGG | 0.156979 | 3.4:-5021621 | MS.gene51658:CDS |
| TGCAATTCATTTACAACTTT+GGG | 0.191576 | 3.4:-5021531 | MS.gene51658:CDS |
| CACATCAAAATCTACAAAAT+TGG | 0.250053 | 3.4:+5021263 | None:intergenic |
| CTGCAATTCATTTACAACTT+TGG | 0.295983 | 3.4:-5021532 | MS.gene51658:CDS |
| TCTTACCTGCCCTTAGTATA+AGG | 0.314556 | 3.4:+5021432 | None:intergenic |
| TTCTTAGAATTTGTTGGGTC+AGG | 0.345198 | 3.4:+5021332 | None:intergenic |
| TGGAGAGAAGGCACGGGAAT+GGG | 0.347886 | 3.4:+5021283 | None:intergenic |
| CAAACATGAGTGAGATTCAT+TGG | 0.355934 | 3.4:-5021358 | MS.gene51658:CDS |
| TGCGTGGCAACCTTATACTA+AGG | 0.364864 | 3.4:-5021442 | MS.gene51658:CDS |
| AGAGGAAAGCTTGAGAAGAT+AGG | 0.378036 | 3.4:-5021410 | MS.gene51658:CDS |
| TATACTTCTTAGAATTTGTT+GGG | 0.390203 | 3.4:+5021327 | None:intergenic |
| CAATGAATCTCACTCATGTT+TGG | 0.429356 | 3.4:+5021359 | None:intergenic |
| ATATACTTCTTAGAATTTGT+TGG | 0.431038 | 3.4:+5021326 | None:intergenic |
| AAAGGGAGGACAACACTTGA+AGG | 0.498268 | 3.4:-5021596 | MS.gene51658:CDS |
| GGGGAGATATGACATACTCT+TGG | 0.503412 | 3.4:+5021303 | None:intergenic |
| TTGGAGAGAAGGCACGGGAA+TGG | 0.503623 | 3.4:+5021282 | None:intergenic |
| GGAGAGAAGGCACGGGAATG+GGG | 0.529940 | 3.4:+5021284 | None:intergenic |
| AATCTACAAAATTGGAGAGA+AGG | 0.536686 | 3.4:+5021271 | None:intergenic |
| CATGTTTGGTGTCTCAACAT+AGG | 0.565946 | 3.4:+5021373 | None:intergenic |
| ACAAAATTGGAGAGAAGGCA+CGG | 0.567717 | 3.4:+5021276 | None:intergenic |
| TATAAGGTTGCCACGCAGGT+AGG | 0.568146 | 3.4:+5021448 | None:intergenic |
| GGCAACCTTATACTAAGGGC+AGG | 0.570742 | 3.4:-5021437 | MS.gene51658:CDS |
| GCAATTCATTTACAACTTTG+GGG | 0.580709 | 3.4:-5021530 | MS.gene51658:CDS |
| TTAGTATAAGGTTGCCACGC+AGG | 0.586370 | 3.4:+5021444 | None:intergenic |
| ATACTAAGGGCAGGTAAGAG+AGG | 0.591856 | 3.4:-5021428 | MS.gene51658:CDS |
| CAGGTAGGAAAAGATACCAT+TGG | 0.607789 | 3.4:+5021463 | None:intergenic |
| CAAAATTGGAGAGAAGGCAC+GGG | 0.629659 | 3.4:+5021277 | None:intergenic |
| GACATCGTTTATATGACCAA+TGG | 0.633538 | 3.4:-5021479 | MS.gene51658:CDS |
| AAGGGAGGACAACACTTGAA+GGG | 0.635043 | 3.4:-5021595 | MS.gene51658:CDS |
| GCGTGGCAACCTTATACTAA+GGG | 0.670757 | 3.4:-5021441 | MS.gene51658:CDS |
| AAGTTGTAAATGAATTGCAG+CGG | 0.683257 | 3.4:+5021535 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTATAATTTTGGAGAA+AGG | - | chr3.4:5021325-5021344 | MS.gene51658:CDS | 15.0% |
| !!! | AATTTATAATTTTGGAGAAA+GGG | - | chr3.4:5021326-5021345 | MS.gene51658:CDS | 15.0% |
| !!! | AGACACTAAATTTATAATTT+TGG | - | chr3.4:5021318-5021337 | MS.gene51658:CDS | 15.0% |
| !! | ATATACTTCTTAGAATTTGT+TGG | + | chr3.4:5021616-5021635 | None:intergenic | 20.0% |
| !!! | TATACTTCTTAGAATTTGTT+GGG | + | chr3.4:5021615-5021634 | None:intergenic | 20.0% |
| ! | TGCAATTCATTTACAACTTT+GGG | - | chr3.4:5021408-5021427 | MS.gene51658:CDS | 25.0% |
| !!! | GAATGTGATTATTTTGATTC+AGG | - | chr3.4:5021436-5021455 | MS.gene51658:CDS | 25.0% |
| AAGTTGTAAATGAATTGCAG+CGG | + | chr3.4:5021407-5021426 | None:intergenic | 30.0% | |
| CTGCAATTCATTTACAACTT+TGG | - | chr3.4:5021407-5021426 | MS.gene51658:CDS | 30.0% | |
| GCAATTCATTTACAACTTTG+GGG | - | chr3.4:5021409-5021428 | MS.gene51658:CDS | 30.0% | |
| !! | AGGGCAAAATAAAAAGTGTT+TGG | - | chr3.4:5021363-5021382 | MS.gene51658:CDS | 30.0% |
| !! | TTATAATTTTGGAGAAAGGG+AGG | - | chr3.4:5021329-5021348 | MS.gene51658:CDS | 30.0% |
| CAAACATGAGTGAGATTCAT+TGG | - | chr3.4:5021581-5021600 | MS.gene51658:CDS | 35.0% | |
| CAATGAATCTCACTCATGTT+TGG | + | chr3.4:5021583-5021602 | None:intergenic | 35.0% | |
| GACATCGTTTATATGACCAA+TGG | - | chr3.4:5021460-5021479 | MS.gene51658:CDS | 35.0% | |
| !! | TTCTTAGAATTTGTTGGGTC+AGG | + | chr3.4:5021610-5021629 | None:intergenic | 35.0% |
| ACAAAATTGGAGAGAAGGCA+CGG | + | chr3.4:5021666-5021685 | None:intergenic | 40.0% | |
| AGAGGAAAGCTTGAGAAGAT+AGG | - | chr3.4:5021529-5021548 | MS.gene51658:CDS | 40.0% | |
| CAGGTAGGAAAAGATACCAT+TGG | + | chr3.4:5021479-5021498 | None:intergenic | 40.0% | |
| TCTTACCTGCCCTTAGTATA+AGG | + | chr3.4:5021510-5021529 | None:intergenic | 40.0% | |
| !! | CATGTTTGGTGTCTCAACAT+AGG | + | chr3.4:5021569-5021588 | None:intergenic | 40.0% |
| !!! | TCTATGTTGTTTTGAGCTGC+AGG | + | chr3.4:5021301-5021320 | None:intergenic | 40.0% |
| AAAGGGAGGACAACACTTGA+AGG | - | chr3.4:5021343-5021362 | MS.gene51658:CDS | 45.0% | |
| AAGGGAGGACAACACTTGAA+GGG | - | chr3.4:5021344-5021363 | MS.gene51658:CDS | 45.0% | |
| ATACTAAGGGCAGGTAAGAG+AGG | - | chr3.4:5021511-5021530 | MS.gene51658:CDS | 45.0% | |
| GCGTGGCAACCTTATACTAA+GGG | - | chr3.4:5021498-5021517 | MS.gene51658:CDS | 45.0% | |
| GGGGAGATATGACATACTCT+TGG | + | chr3.4:5021639-5021658 | None:intergenic | 45.0% | |
| TGCGTGGCAACCTTATACTA+AGG | - | chr3.4:5021497-5021516 | MS.gene51658:CDS | 45.0% | |
| TTAGTATAAGGTTGCCACGC+AGG | + | chr3.4:5021498-5021517 | None:intergenic | 45.0% | |
| ! | CAAAATTGGAGAGAAGGCAC+GGG | + | chr3.4:5021665-5021684 | None:intergenic | 45.0% |
| GGCAACCTTATACTAAGGGC+AGG | - | chr3.4:5021502-5021521 | MS.gene51658:CDS | 50.0% | |
| TATAAGGTTGCCACGCAGGT+AGG | + | chr3.4:5021494-5021513 | None:intergenic | 50.0% | |
| ! | GGTATCTTTTCCTACCTGCG+TGG | - | chr3.4:5021481-5021500 | MS.gene51658:CDS | 50.0% |
| ! | TGGAGAGAAGGCACGGGAAT+GGG | + | chr3.4:5021659-5021678 | None:intergenic | 55.0% |
| ! | TTGGAGAGAAGGCACGGGAA+TGG | + | chr3.4:5021660-5021679 | None:intergenic | 55.0% |
| ! | GGAGAGAAGGCACGGGAATG+GGG | + | chr3.4:5021658-5021677 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 5021274 | 5021687 | 5021274 | ID=MS.gene51658 |
| chr3.4 | mRNA | 5021274 | 5021687 | 5021274 | ID=MS.gene51658.t1;Parent=MS.gene51658 |
| chr3.4 | exon | 5021274 | 5021687 | 5021274 | ID=MS.gene51658.t1.exon1;Parent=MS.gene51658.t1 |
| chr3.4 | CDS | 5021274 | 5021687 | 5021274 | ID=cds.MS.gene51658.t1;Parent=MS.gene51658.t1 |
| Gene Sequence |
| Protein sequence |
