AlfalfaGEDB | MS.gene53056

M. sativa cultivar XinJiangDaYe / MS.gene53056

KEGG: -
GO: GO:0005575 GO:0005576 GO:0008150 GO:0009664 GO:0009987 GO:0016043 GO:0045229 GO:0071554 GO:0071555 GO:0071669 GO:0071840 GO:0110165
PFAM: PF01357.24 PF03330.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53056.t1	XP_003613849.1	97.6	248	6	0	1	248	1	248	4.00E-140	507.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53056.t1	Q9LDR9	84.3	249	38	1	1	248	1	249	5.9e-124	444.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53056.t1	G7KGP1	97.6	248	6	0	1	248	1	248	2.9e-140	507.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049151	MS.gene53056	0.807208	5.66E-50	-1.69E-46
MS.gene049297	MS.gene53056	0.844899	5.68E-59	-1.69E-46
MS.gene049491	MS.gene53056	0.808718	2.70E-50	-1.69E-46
MS.gene049758	MS.gene53056	0.863269	2.81E-64	-1.69E-46
MS.gene050076	MS.gene53056	0.818195	2.23E-52	-1.69E-46
MS.gene050099	MS.gene53056	0.801487	8.80E-49	-1.69E-46
MS.gene050128	MS.gene53056	0.803714	3.06E-49	-1.69E-46
MS.gene050301	MS.gene53056	0.827618	1.42E-54	-1.69E-46
MS.gene050558	MS.gene53056	0.805777	1.13E-49	-1.69E-46
MS.gene050623	MS.gene53056	-0.808391	3.17E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene53056	MS.gene24107	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53056.t1	MTR_5g041700	97.581	248	6	0	1	248	1	248	0.0	496
MS.gene53056.t1	MTR_4g081950	87.045	247	32	0	1	247	37	283	3.56e-164	455
MS.gene53056.t1	MTR_3g094040	80.165	242	48	0	6	247	12	253	1.06e-146	410
MS.gene53056.t1	MTR_3g094060	80.165	242	48	0	6	247	12	253	1.47e-146	409
MS.gene53056.t1	MTR_3g435430	80.769	234	45	0	14	247	14	247	1.15e-145	407
MS.gene53056.t1	MTR_5g075320	73.984	246	63	1	3	247	6	251	4.17e-139	390
MS.gene53056.t1	MTR_7g011060	76.305	249	48	3	1	247	1	240	2.34e-136	383
MS.gene53056.t1	MTR_3g466760	75.709	247	60	0	1	247	14	260	2.55e-133	376
MS.gene53056.t1	MTR_3g466790	75.304	247	61	0	1	247	8	254	8.28e-133	375
MS.gene53056.t1	MTR_5g079950	74.236	229	57	1	22	248	30	258	2.91e-126	358

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53056.t1	AT2G03090	83.065	248	42	0	1	248	6	253	3.30e-155	431
MS.gene53056.t1	AT1G69530	82.800	250	41	2	1	248	1	250	1.21e-152	425
MS.gene53056.t1	AT1G69530	82.800	250	41	2	1	248	1	250	1.21e-152	425
MS.gene53056.t1	AT1G69530	81.600	250	44	2	1	248	1	250	3.08e-150	420
MS.gene53056.t1	AT1G26770	84.337	249	38	1	1	248	1	249	6.35e-146	408
MS.gene53056.t1	AT1G26770	84.337	249	38	1	1	248	11	259	7.53e-146	408
MS.gene53056.t1	AT5G56320	76.829	246	56	1	3	248	8	252	2.14e-139	392
MS.gene53056.t1	AT5G56320	76.829	246	56	1	3	248	8	252	2.14e-139	392
MS.gene53056.t1	AT5G56320	76.829	246	56	1	3	248	8	252	1.88e-138	391
MS.gene53056.t1	AT2G40610	70.850	247	68	2	2	247	10	253	5.23e-133	375

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Find 73 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCTATACAGTCAAGGTTA+TGG	0.427075	5.4:-30612521	MS.gene53056:CDS
AAGTGATGCATCAGGAACAA+TGG	0.564601	5.4:-30612674	MS.gene53056:CDS
AATGCACATGCAACTTTCTA+TGG	0.254508	5.4:-30612700	MS.gene53056:CDS
AATGCCTTGCCTAATAACAA+TGG	0.606510	5.4:-30612353	MS.gene53056:CDS
AATTCCTCCCCTTCTTCTGC+AGG	0.397486	5.4:+30611584	None:intergenic
ACAATGCTGCCCCAGCTGGT+TGG	0.464148	5.4:-30611344	MS.gene53056:CDS
ACCAAAAGACCAACCAGCTG+GGG	0.625454	5.4:+30611335	None:intergenic
ACCAGCTGGGGCAGCATTGT+TGG	0.398932	5.4:+30611347	None:intergenic
ACGGAATTGGGCACCAGTGT+AGG	0.562519	5.4:+30611308	None:intergenic
AGAATTGCTCAATACAAAGC+TGG	0.532239	5.4:-30612275	MS.gene53056:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTTATCTAGCTAGTCAA+TGG	-	chr5.4:30612068-30612087	MS.gene53056:intron	30.0%
ACTTTATCTAGCTAGTCAAT+GGG	-	chr5.4:30612069-30612088	MS.gene53056:intron	30.0%
ATTTACCTTCTGTAAGAAAC+AGG	+	chr5.4:30611841-30611860	None:intergenic	30.0%
GTACATTCAGTAGCTATTAA+AGG	-	chr5.4:30612591-30612610	MS.gene53056:intron	30.0%
AACCTATACAGTCAAGGTTA+TGG	-	chr5.4:30611562-30611581	MS.gene53056:CDS	35.0%
AATGCACATGCAACTTTCTA+TGG	-	chr5.4:30611383-30611402	MS.gene53056:CDS	35.0%
AATGCCTTGCCTAATAACAA+TGG	-	chr5.4:30611730-30611749	MS.gene53056:intron	35.0%
AGAATTGCTCAATACAAAGC+TGG	-	chr5.4:30611808-30611827	MS.gene53056:intron	35.0%
ATGCCAATGTCTAGAAATTG+GGG	-	chr5.4:30612630-30612649	MS.gene53056:intron	35.0%
ATTGTTGTGTGGATTTGTGT+AGG	-	chr5.4:30612472-30612491	MS.gene53056:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	30611305	30612800	30611305	ID=MS.gene53056
chr5.4	mRNA	30611305	30612800	30611305	ID=MS.gene53056.t1;Parent=MS.gene53056
chr5.4	exon	30612674	30612800	30612674	ID=MS.gene53056.t1.exon1;Parent=MS.gene53056.t1
chr5.4	CDS	30612674	30612800	30612674	ID=cds.MS.gene53056.t1;Parent=MS.gene53056.t1
chr5.4	exon	30612251	30612563	30612251	ID=MS.gene53056.t1.exon2;Parent=MS.gene53056.t1
chr5.4	CDS	30612251	30612563	30612251	ID=cds.MS.gene53056.t1;Parent=MS.gene53056.t1
chr5.4	exon	30611305	30611611	30611305	ID=MS.gene53056.t1.exon3;Parent=MS.gene53056.t1
chr5.4	CDS	30611305	30611611	30611305	ID=cds.MS.gene53056.t1;Parent=MS.gene53056.t1

Gene Sequence

>MS.gene53056

ATGGCTCTCATTGGGTTGTTCTTCATGGTGTTTCTCACTATGTTTTCATCTGCTTATGGTTATGGTGGAGGATGGGTTAATGCACATGCAACTTTCTATGGAGGAAGTGATGCATCAGGAACAATGGGTATGTATGCAACAATAATACATAAAAAAAAAGTTAACATTTTTATACTATTTAAGCAATTTGGACCAACCCCTTTTGACTAATACGTTTAATATTGAAATTTTGTTTAGGTGGTGCATGTGGATATGGAAACCTATACAGTCAAGGTTATGGAACAAACACTGCTGCTTTAAGCACAGCTTTGTTCAACAGTGGTTTGAGCTGTGGTTCTTGCTTTGAGATTAGGTGTGCTGGTGACCGTAAATGGTGTCTTCCTGGCTCTATTTTGGTCACTGCTACTAATTTCTGTCCACCAAACAATGCCTTGCCTAATAACAATGGAGGATGGTGTAACCCTCCTTTGCAACACTTTGATCTTGCTCAACCTGTTTTTCTTAGAATTGCTCAATACAAAGCTGGAATTGTTCCTGTTTCTTACAGAAGGTAAATTTTTTAGTACTTTTTTTTTCTTCACTTTTTGTTGCATTTTGAGATTAAATTTGTGTAATGGGTTGGACCTTCATGTCACTTTCAACTTGTAAATGGTTTTTTTTTTTTTTTAATTTAATTTAATTTAATTTTTAATCACATGTATTTGGAAGTTTCCCTACTTTTTGTTTTATGCTAGAGTGTAATTTCTTTTAGTTACAAGATTTTAACTTTATCTAGCTAGTCAATGGGGTTGGTAATTTTGTAGTTAAAGTACTGTATTTTTTTCTCTCTAAATGGGTTGATGAATGAAACTACACTATTAATTAACTTTTGAAGATTATTTATATGTATAATATTAAAATAAATTTTGTTATATTTGTTTGATGAAATTATCTATCTTTTTATGTAGTACCCTTCAATGTTAATTGTACCATTTCACCTCTCTAATAAATAATTTTAAATGTTTTTTTTTCCAAAACAATAATTAATATTAGTACTTTTAACTTTGACACAACAATTAAAAAATAAAATATTTTTTATTAAAAAAAGGACACTTATATAGAAAAAGAAGCAATGAATAACCAAGTTGTAAGTAATTTATACTTGTGTTTATAAAAAAGAAGTAATTTATTGTTGTGTGGATTTGTGTAGGGTACCCTGCAGAAGAAGGGGAGGAATTAGGTTCACAATCAATGGACATTCCTACTTCAACTTAGTTCTAGTAACAAATGTGGGTGGTGCTGGAGATGTACATTCAGTAGCTATTAAAGGGTCAAGAACAGGGTGGATGCCAATGTCTAGAAATTGGGGTCAAAATTGGCAAAGCAACAATTATCTTAATGGCCAAGCTTTGTCTTTCAAGGTTACAACAAGTGATGGCAAAACAGTCATTTCCAACAATGCTGCCCCAGCTGGTTGGTCTTTTGGTCAGACCTACACTGGTGCCCAATTCCGTTAG

Protein sequence

>MS.gene53056.t1

MALIGLFFMVFLTMFSSAYGYGGGWVNAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIRCAGDRKWCLPGSILVTATNFCPPNNALPNNNGGWCNPPLQHFDLAQPVFLRIAQYKAGIVPVSYRRVPCRRRGGIRFTINGHSYFNLVLVTNVGGAGDVHSVAIKGSRTGWMPMSRNWGQNWQSNNYLNGQALSFKVTTSDGKTVISNNAAPAGWSFGQTYTGAQFR