AlfalfaGEDB | MS.gene54119

M. sativa cultivar XinJiangDaYe / MS.gene54119

KEGG: -
GO: -
PFAM: PF08246.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54119.t1	PNX71632.1	58.3	72	30	0	22	93	25	96	2.50E-15	91.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54119.t1	Q9LXW3	41.8	67	39	0	27	93	36	102	1.5e-08	60.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54119.t1	A0A2K3KZC1	58.3	72	30	0	22	93	25	96	1.8e-15	91.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene54119	-0.809661	1.69E-50	-1.69E-46
MS.gene049217	MS.gene54119	-0.827121	1.86E-54	-1.69E-46
MS.gene049222	MS.gene54119	-0.845458	4.01E-59	-1.69E-46
MS.gene049285	MS.gene54119	-0.816305	5.92E-52	-1.69E-46
MS.gene049633	MS.gene54119	-0.813756	2.18E-51	-1.69E-46
MS.gene050163	MS.gene54119	-0.823656	1.23E-53	-1.69E-46
MS.gene050165	MS.gene54119	-0.83529	1.83E-56	-1.69E-46
MS.gene050323	MS.gene54119	-0.803402	3.55E-49	-1.69E-46
MS.gene050444	MS.gene54119	-0.862932	3.57E-64	-1.69E-46
MS.gene050504	MS.gene54119	-0.832884	7.33E-56	-1.69E-46
MS.gene050612	MS.gene54119	-0.816438	5.53E-52	-1.69E-46
MS.gene050675	MS.gene54119	-0.801291	9.65E-49	-1.69E-46
MS.gene05079	MS.gene54119	0.8068	6.90E-50	-1.69E-46
MS.gene05080	MS.gene54119	-0.821375	4.17E-53	-1.69E-46
MS.gene050868	MS.gene54119	-0.839863	1.23E-57	-1.69E-46
MS.gene050871	MS.gene54119	-0.840145	1.04E-57	-1.69E-46
MS.gene050876	MS.gene54119	-0.815618	8.43E-52	-1.69E-46
MS.gene050904	MS.gene54119	-0.84718	1.36E-59	-1.69E-46
MS.gene051138	MS.gene54119	0.803299	3.73E-49	-1.69E-46
MS.gene051268	MS.gene54119	-0.818766	1.65E-52	-1.69E-46
MS.gene051317	MS.gene54119	-0.815446	9.21E-52	-1.69E-46
MS.gene051810	MS.gene54119	-0.807641	4.58E-50	-1.69E-46
MS.gene051838	MS.gene54119	-0.825578	4.34E-54	-1.69E-46
MS.gene051949	MS.gene54119	-0.814844	1.25E-51	-1.69E-46
MS.gene052129	MS.gene54119	0.812324	4.49E-51	-1.69E-46
MS.gene052164	MS.gene54119	-0.827876	1.23E-54	-1.69E-46
MS.gene052870	MS.gene54119	-0.840707	7.39E-58	-1.69E-46
MS.gene052872	MS.gene54119	-0.845581	3.71E-59	-1.69E-46
MS.gene052966	MS.gene54119	-0.813424	2.58E-51	-1.69E-46
MS.gene053203	MS.gene54119	-0.808039	3.77E-50	-1.69E-46
MS.gene053632	MS.gene54119	-0.839581	1.45E-57	-1.69E-46
MS.gene053776	MS.gene54119	0.83283	7.56E-56	-1.69E-46
MS.gene053824	MS.gene54119	-0.824243	8.97E-54	-1.69E-46
MS.gene054600	MS.gene54119	-0.804009	2.65E-49	-1.69E-46
MS.gene054706	MS.gene54119	0.821215	4.55E-53	-1.69E-46
MS.gene05470	MS.gene54119	-0.814584	1.43E-51	-1.69E-46
MS.gene05494	MS.gene54119	-0.80688	6.64E-50	-1.69E-46
MS.gene054954	MS.gene54119	-0.80668	7.31E-50	-1.69E-46
MS.gene055502	MS.gene54119	-0.80721	5.65E-50	-1.69E-46
MS.gene055577	MS.gene54119	-0.853362	2.51E-61	-1.69E-46
MS.gene055748	MS.gene54119	-0.832033	1.19E-55	-1.69E-46
MS.gene055751	MS.gene54119	-0.832751	7.90E-56	-1.69E-46
MS.gene05589	MS.gene54119	-0.826514	2.60E-54	-1.69E-46
MS.gene055941	MS.gene54119	-0.828519	8.59E-55	-1.69E-46
MS.gene056283	MS.gene54119	-0.818034	2.42E-52	-1.69E-46
MS.gene056387	MS.gene54119	-0.836685	8.09E-57	-1.69E-46
MS.gene056449	MS.gene54119	-0.828773	7.46E-55	-1.69E-46
MS.gene056504	MS.gene54119	-0.810526	1.10E-50	-1.69E-46
MS.gene056510	MS.gene54119	-0.813499	2.48E-51	-1.69E-46
MS.gene056657	MS.gene54119	-0.800392	1.47E-48	-1.69E-46
MS.gene056903	MS.gene54119	-0.823267	1.52E-53	-1.69E-46
MS.gene057857	MS.gene54119	-0.809507	1.83E-50	-1.69E-46
MS.gene058773	MS.gene54119	-0.838957	2.11E-57	-1.69E-46
MS.gene058838	MS.gene54119	-0.800267	1.56E-48	-1.69E-46
MS.gene059022	MS.gene54119	-0.820706	5.95E-53	-1.69E-46
MS.gene059023	MS.gene54119	-0.841386	4.90E-58	-1.69E-46
MS.gene059102	MS.gene54119	0.828576	8.33E-55	-1.69E-46
MS.gene059677	MS.gene54119	-0.827514	1.50E-54	-1.69E-46
MS.gene059718	MS.gene54119	-0.830068	3.62E-55	-1.69E-46
MS.gene059865	MS.gene54119	-0.808506	3.00E-50	-1.69E-46
MS.gene06000	MS.gene54119	-0.808441	3.09E-50	-1.69E-46
MS.gene060703	MS.gene54119	-0.801513	8.69E-49	-1.69E-46
MS.gene060762	MS.gene54119	-0.851399	9.10E-61	-1.69E-46
MS.gene060982	MS.gene54119	-0.800468	1.42E-48	-1.69E-46
MS.gene06111	MS.gene54119	-0.803487	3.40E-49	-1.69E-46
MS.gene061150	MS.gene54119	-0.802588	5.23E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54119.t1	MTR_7g086240	52.500	80	34	1	11	90	17	92	1.29e-23	87.8
MS.gene54119.t1	MTR_3g112430	55.556	63	28	0	27	89	31	93	2.81e-18	79.3
MS.gene54119.t1	MTR_2g064475	54.688	64	29	0	33	96	17	80	8.70e-17	74.7
MS.gene54119.t1	MTR_2g067560	48.438	64	33	0	32	95	16	79	6.75e-15	69.7
MS.gene54119.t1	MTR_2g067900	49.206	63	32	0	33	95	17	79	6.16e-14	66.6
MS.gene54119.t1	MTR_2g066980	48.438	64	33	0	33	96	35	98	3.65e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54119.t1	AT1G09850	45.833	72	38	1	27	97	27	98	8.47e-16	72.4
MS.gene54119.t1	AT3G43960	41.791	67	39	0	27	93	36	102	6.83e-13	63.9
MS.gene54119.t1	AT4G35350	51.515	66	31	1	28	93	47	111	9.06e-13	63.5
MS.gene54119.t1	AT4G35350	51.515	66	31	1	28	93	47	111	9.83e-13	63.2

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAATGGAACAACCATAAT+TGG	0.195557	2.2:+23647935	None:intergenic
TTGGTTGTGTTGAGTGATTC+TGG	0.214560	2.2:+23648025	None:intergenic
GAGGAATTTACGTCCAATTA+TGG	0.252320	2.2:-23647948	MS.gene54119:CDS
AAACTTCATTGATGTTTGAC+TGG	0.385671	2.2:+23647867	None:intergenic
TTCTTGAAGCTTGGAGTTGG+AGG	0.392872	2.2:+23648283	None:intergenic
AGAATAACAAACCGATGGTT+GGG	0.404177	2.2:+23648250	None:intergenic
CTGTTCTTGAAGCTTGGAGT+TGG	0.411197	2.2:+23648280	None:intergenic
CCGGATGATCTTGAAAAGTT+AGG	0.423858	2.2:-23647897	MS.gene54119:CDS
TCCATGAAATATTTGAAAAG+TGG	0.434083	2.2:-23648124	MS.gene54119:CDS
AGGAATCTGTTCTTGAAGCT+TGG	0.443942	2.2:+23648274	None:intergenic
TGAATATAAGGCTCCTGGTT+CGG	0.465353	2.2:-23648150	MS.gene54119:intron
AATTGTGAATATAAGGCTCC+TGG	0.479700	2.2:-23648155	MS.gene54119:intron
CGAAGGTGAGATCAGAGAAC+TGG	0.488939	2.2:+23647970	None:intergenic
TGATTTCAACGATGTCCATC+CGG	0.494998	2.2:-23647916	MS.gene54119:CDS
TAGAATAACAAACCGATGGT+TGG	0.501861	2.2:+23648249	None:intergenic
GTTCTCTGATCTCACCTTCG+AGG	0.507841	2.2:-23647967	MS.gene54119:CDS
ATGGACATCGTTGAAATCAA+TGG	0.510440	2.2:+23647920	None:intergenic
CGACAAAACATATTCTTCAA+AGG	0.513620	2.2:-23648090	MS.gene54119:CDS
AACTTCATTGATGTTTGACT+GGG	0.536300	2.2:+23647868	None:intergenic
GAATAACAAACCGATGGTTG+GGG	0.564507	2.2:+23648251	None:intergenic
ACAGATTCCTCCCCAACCAT+CGG	0.584110	2.2:-23648261	MS.gene54119:intron
ATTTCATGGATGTCCGAACC+AGG	0.584248	2.2:+23648137	None:intergenic
TGAGATCAGAGAACTGGTTG+AGG	0.597092	2.2:+23647976	None:intergenic
AATATTTGAAAAGTGGTGCA+AGG	0.602264	2.2:-23648117	MS.gene54119:CDS
TAACAAACCGATGGTTGGGG+AGG	0.622332	2.2:+23648254	None:intergenic
ATTGGACGTAAATTCCTCGA+AGG	0.638330	2.2:+23647953	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTTAATTGTGAATATA+AGG	-	chr2.2:23648015-23648034	MS.gene54119:CDS	10.0%
!	AAAATCATGAGAGAAATTGA+AGG	+	chr2.2:23647956-23647975	None:intergenic	25.0%
!	AAATCATGAGAGAAATTGAA+GGG	+	chr2.2:23647955-23647974	None:intergenic	25.0%
!	TCCATGAAATATTTGAAAAG+TGG	-	chr2.2:23648053-23648072	MS.gene54119:CDS	25.0%
!!!	ACCACTTTTCAAATATTTCA+TGG	+	chr2.2:23648057-23648076	None:intergenic	25.0%
	AATATTTGAAAAGTGGTGCA+AGG	-	chr2.2:23648060-23648079	MS.gene54119:CDS	30.0%
	ATCAATGGAACAACCATAAT+TGG	+	chr2.2:23648245-23648264	None:intergenic	30.0%
	CGACAAAACATATTCTTCAA+AGG	-	chr2.2:23648087-23648106	MS.gene54119:CDS	30.0%
!	AAAAATTCTGGTTACACTCT+AGG	-	chr2.2:23648178-23648197	MS.gene54119:intron	30.0%
!!	TGTAACCAGAATTTTTCAGT+TGG	+	chr2.2:23648174-23648193	None:intergenic	30.0%
	AATTGTGAATATAAGGCTCC+TGG	-	chr2.2:23648022-23648041	MS.gene54119:CDS	35.0%
	AGAATAACAAACCGATGGTT+GGG	+	chr2.2:23647930-23647949	None:intergenic	35.0%
	ATGGACATCGTTGAAATCAA+TGG	+	chr2.2:23648260-23648279	None:intergenic	35.0%
	CAAAGGAAGAAAAACAGTAC+AGG	-	chr2.2:23648104-23648123	MS.gene54119:CDS	35.0%
	CACAACCAACTGAAAAATTC+TGG	-	chr2.2:23648166-23648185	MS.gene54119:intron	35.0%
	GAGGAATTTACGTCCAATTA+TGG	-	chr2.2:23648229-23648248	MS.gene54119:intron	35.0%
	TAGAATAACAAACCGATGGT+TGG	+	chr2.2:23647931-23647950	None:intergenic	35.0%
!!	CCTAACTTTTCAAGATCATC+CGG	+	chr2.2:23648283-23648302	None:intergenic	35.0%
	AGGAATCTGTTCTTGAAGCT+TGG	+	chr2.2:23647906-23647925	None:intergenic	40.0%
	ATTGGACGTAAATTCCTCGA+AGG	+	chr2.2:23648227-23648246	None:intergenic	40.0%
	CCGGATGATCTTGAAAAGTT+AGG	-	chr2.2:23648280-23648299	MS.gene54119:CDS	40.0%
	GAATAACAAACCGATGGTTG+GGG	+	chr2.2:23647929-23647948	None:intergenic	40.0%
	GGGATAGAATAACAAACCGA+TGG	+	chr2.2:23647935-23647954	None:intergenic	40.0%
	TGAATATAAGGCTCCTGGTT+CGG	-	chr2.2:23648027-23648046	MS.gene54119:CDS	40.0%
	TGATTTCAACGATGTCCATC+CGG	-	chr2.2:23648261-23648280	MS.gene54119:intron	40.0%
	TTGGTTGTGTTGAGTGATTC+TGG	+	chr2.2:23648155-23648174	None:intergenic	40.0%
!!	ACTTTTCAAGATCATCCGGA+TGG	+	chr2.2:23648279-23648298	None:intergenic	40.0%
	ATTTCATGGATGTCCGAACC+AGG	+	chr2.2:23648043-23648062	None:intergenic	45.0%
	CTGTTCTTGAAGCTTGGAGT+TGG	+	chr2.2:23647900-23647919	None:intergenic	45.0%
	TGAGATCAGAGAACTGGTTG+AGG	+	chr2.2:23648204-23648223	None:intergenic	45.0%
	TTCTTGAAGCTTGGAGTTGG+AGG	+	chr2.2:23647897-23647916	None:intergenic	45.0%
	ACAGATTCCTCCCCAACCAT+CGG	-	chr2.2:23647916-23647935	MS.gene54119:CDS	50.0%
	CGAAGGTGAGATCAGAGAAC+TGG	+	chr2.2:23648210-23648229	None:intergenic	50.0%
	GTTCTCTGATCTCACCTTCG+AGG	-	chr2.2:23648210-23648229	MS.gene54119:intron	50.0%
	TAACAAACCGATGGTTGGGG+AGG	+	chr2.2:23647926-23647945	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	23647872	23648327	23647872	ID=MS.gene54119
chr2.2	mRNA	23647872	23648327	23647872	ID=MS.gene54119.t1;Parent=MS.gene54119
chr2.2	exon	23648262	23648327	23648262	ID=MS.gene54119.t1.exon1;Parent=MS.gene54119.t1
chr2.2	CDS	23648262	23648327	23648262	ID=cds.MS.gene54119.t1;Parent=MS.gene54119.t1
chr2.2	exon	23647872	23648162	23647872	ID=MS.gene54119.t1.exon2;Parent=MS.gene54119.t1
chr2.2	CDS	23647872	23648162	23647872	ID=cds.MS.gene54119.t1;Parent=MS.gene54119.t1

Gene Sequence

>MS.gene54119

ATGGAAGTAAACAAAGAGCTAACCTCCAACTCCAAGCTTCAAGAACAGATTCCTCCCCAACCATCGGTTTGTTATTCTATCCCTTCAATTTCTCTCATGATTTTTGTTTAATTGATTGATTTTCTTTGTTAATTTATACTTTGTAATTTTAATTGTGAATATAAGGCTCCTGGTTCGGACATCCATGAAATATTTGAAAAGTGGTGCAAGGAATTCGACAAAACATATTCTTCAAAGGAAGAAAAACAGTACAGGTTCAAAGTGTTTGAAGAAAGATATGCCAGAATCACTCAACACAACCAACTGAAAAATTCTGGTTACACTCTAGGCCTCAACCAGTTCTCTGATCTCACCTTCGAGGAATTTACGTCCAATTATGGTTGTTCCATTGATTTCAACGATGTCCATCCGGATGATCTTGAAAAGTTAGGCATTAACCCAGTCAAACATCAATGA

Protein sequence

>MS.gene54119.t1

MEVNKELTSNSKLQEQIPPQPSAPGSDIHEIFEKWCKEFDKTYSSKEEKQYRFKVFEERYARITQHNQLKNSGYTLGLNQFSDLTFEEFTSNYGCSIDFNDVHPDDLEKLGINPVKHQ