AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55759


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55759.t1 MTR_8g099085 99.522 209 1 0 14 222 135 343 2.00e-148 417
MS.gene55759.t1 MTR_1g099420 73.148 216 58 0 7 222 127 342 5.61e-116 334
MS.gene55759.t1 MTR_8g467540 29.469 207 127 4 25 218 120 320 1.15e-17 80.5
MS.gene55759.t1 MTR_8g467540 29.469 207 127 4 25 218 120 320 1.15e-17 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55759.t1 AT5G60750 79.524 210 42 1 14 222 138 347 8.00e-116 334
MS.gene55759.t1 AT1G14270 45.882 85 46 0 134 218 124 208 1.51e-18 81.3
MS.gene55759.t1 AT1G14270 45.882 85 46 0 134 218 124 208 1.51e-18 81.3
MS.gene55759.t1 AT1G14270 45.882 85 46 0 134 218 144 228 2.58e-18 80.9
MS.gene55759.t1 AT1G14270 45.882 85 46 0 134 218 250 334 1.09e-17 80.9
MS.gene55759.t1 AT2G20725 41.573 89 48 1 130 218 215 299 3.29e-13 67.8

Find 63 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAGTGACAAGGCTAAAT+AGG 0.223638 4.3:-5125464 None:intergenic
CAATGTTGTTGCATAGTCTT+TGG 0.238859 4.3:+5125967 MS.gene55759:CDS
ACACCACACAGGCATGTATT+TGG 0.285573 4.3:-5125820 None:intergenic
AAGGATTCTTTGACATTTAG+AGG 0.293855 4.3:+5125435 MS.gene55759:CDS
ATGCTGCCACTTATATTTCT+TGG 0.297795 4.3:+5125897 MS.gene55759:CDS
CCTTGCTGAAGCCAGTTATA+TGG 0.298053 4.3:-5125416 None:intergenic
AGTTTAGCCTGGAAGGAAAA+TGG 0.299581 4.3:+5125565 MS.gene55759:CDS
GCCTGTGTGGTGTGCAATAC+TGG 0.349411 4.3:+5125830 MS.gene55759:CDS
TGCTGCCACTTATATTTCTT+GGG 0.375579 4.3:+5125898 MS.gene55759:CDS
TCATGTTTCCTCTTGTCAAT+CGG 0.417015 4.3:+5125613 MS.gene55759:CDS
ATCAGTGACAAGGCTAAATA+GGG 0.430586 4.3:-5125463 None:intergenic
AGACTATGCAACAACATTGA+TGG 0.439339 4.3:-5125963 None:intergenic
TCCGTCCCCTTCCACCCGAC+TGG 0.444389 4.3:+5125538 MS.gene55759:CDS
GCCACTTATATTTCTTGGGA+TGG 0.450363 4.3:+5125902 MS.gene55759:CDS
CTTGCTGAAGCCAGTTATAT+GGG 0.459032 4.3:-5125415 None:intergenic
TTCTTTGACATTTAGAGGGC+AGG 0.461094 4.3:+5125440 MS.gene55759:CDS
TATATTTCTTGGGATGGTGA+TGG 0.475959 4.3:+5125908 MS.gene55759:CDS
TCGATGGCAGGATGGGTAGT+AGG 0.477125 4.3:-5125656 None:intergenic
TGATTGTTCAACACTTGAAA+GGG 0.482634 4.3:-5125688 None:intergenic
TTGATTGTTCAACACTTGAA+AGG 0.487389 4.3:-5125689 None:intergenic
CGACTGGTTCAAGTTTAGCC+TGG 0.494889 4.3:+5125554 MS.gene55759:CDS
ATATTTCTTGGGATGGTGAT+GGG 0.495092 4.3:+5125909 MS.gene55759:CDS
ATCGGTCTGCGCTCCTGTGT+GGG 0.497992 4.3:+5125758 MS.gene55759:CDS
CTTGAACCAGTCGGGTGGAA+GGG 0.500064 4.3:-5125544 None:intergenic
ACCTGTGTCGATGGCAGGAT+GGG 0.500774 4.3:-5125663 None:intergenic
TTGAACCAGTCGGGTGGAAG+GGG 0.510746 4.3:-5125543 None:intergenic
CCAGTTATATGGGCTGCAAA+AGG 0.512015 4.3:-5125405 None:intergenic
TATCGGTCTGCGCTCCTGTG+TGG 0.516259 4.3:+5125757 MS.gene55759:CDS
GACCTGTGTCGATGGCAGGA+TGG 0.530416 4.3:-5125664 None:intergenic
GATGTAACTGAAGGACTAGC+TGG 0.531776 4.3:+5125483 MS.gene55759:CDS
ACTTGAACCAGTCGGGTGGA+AGG 0.533549 4.3:-5125545 None:intergenic
AGGCTAAACTTGAACCAGTC+GGG 0.535684 4.3:-5125552 None:intergenic
GTTGTATGCAATTGTAGTAT+CGG 0.544728 4.3:+5125740 MS.gene55759:CDS
GGGTGACCTGTGTCGATGGC+AGG 0.545730 4.3:-5125668 None:intergenic
AATATGGTCGTTGCACTCCA+TGG 0.550589 4.3:-5125140 None:intergenic
TGGTTCAAGTTTAGCCTGGA+AGG 0.555909 4.3:+5125558 MS.gene55759:CDS
ACCATCCCAAGAAATATAAG+TGG 0.557728 4.3:-5125903 None:intergenic
TTGAACAATCAATAAAAGCA+AGG 0.561023 4.3:+5125700 MS.gene55759:CDS
GCATGTATTTGGTAAGTGAC+GGG 0.563519 4.3:-5125809 None:intergenic
ACCAGTCGGGTGGAAGGGGA+CGG 0.564379 4.3:-5125539 None:intergenic
GGCATGTATTTGGTAAGTGA+CGG 0.567093 4.3:-5125810 None:intergenic
CAGGCTAAACTTGAACCAGT+CGG 0.579922 4.3:-5125553 None:intergenic
AGGATTCTTTGACATTTAGA+GGG 0.596115 4.3:+5125436 MS.gene55759:CDS
GTGTGATATTTACACGGTCA+AGG 0.600158 4.3:+5125931 MS.gene55759:CDS
TTGTTGCATAGTCTTTGGAA+TGG 0.609310 4.3:+5125972 MS.gene55759:CDS
TGAGAGAGCCGATTGACAAG+AGG 0.610131 4.3:-5125621 None:intergenic
GCAGGATGGGTAGTAGGTCA+AGG 0.610496 4.3:-5125650 None:intergenic
GCATACAACAACATTGCCAC+AGG 0.610600 4.3:-5125726 None:intergenic
TGAACAATCAATAAAAGCAA+GGG 0.613230 4.3:+5125701 MS.gene55759:CDS
GGTCTGCGCTCCTGTGTGGG+AGG 0.621158 4.3:+5125761 MS.gene55759:CDS
GTAATCGATGGGTTGTACCA+TGG 0.622454 4.3:+5125123 None:intergenic
CCATATAACTGGCTTCAGCA+AGG 0.626497 4.3:+5125416 MS.gene55759:CDS
CTTGTCACTGATGTAACTGA+AGG 0.627885 4.3:+5125474 MS.gene55759:CDS
ACCCATCCTGCCATCGACAC+AGG 0.628035 4.3:+5125662 MS.gene55759:CDS
ACCAGTATTGCACACCACAC+AGG 0.628546 4.3:-5125831 None:intergenic
CTAAACTTGAACCAGTCGGG+TGG 0.632925 4.3:-5125549 None:intergenic
AATAAAAGCAAGGGATCCTG+TGG 0.652461 4.3:+5125710 MS.gene55759:CDS
GAAAGGGTGACCTGTGTCGA+TGG 0.653532 4.3:-5125672 None:intergenic
CTTCAGTTACATCAGTGACA+AGG 0.657659 4.3:-5125473 None:intergenic
AAAACTATTTCCTCCCACAC+AGG 0.659992 4.3:-5125771 None:intergenic
TTACCAAATACATGCCTGTG+TGG 0.698661 4.3:+5125817 MS.gene55759:CDS
TGATGGGTGTGATATTTACA+CGG 0.748257 4.3:+5125925 MS.gene55759:CDS
GTGACGGGAGTAGAAAACCG+CGG 0.770907 4.3:-5125794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAACACTTACTTTTAATA+TGG - chr4.3:5125159-5125178 None:intergenic 20.0%
! TGAACAATCAATAAAAGCAA+GGG + chr4.3:5125701-5125720 MS.gene55759:CDS 25.0%
! TTGAACAATCAATAAAAGCA+AGG + chr4.3:5125700-5125719 MS.gene55759:CDS 25.0%
AAGGATTCTTTGACATTTAG+AGG + chr4.3:5125435-5125454 MS.gene55759:CDS 30.0%
AGGATTCTTTGACATTTAGA+GGG + chr4.3:5125436-5125455 MS.gene55759:CDS 30.0%
ATTCCTCGCAGTTAAAATTT+AGG + chr4.3:5125229-5125248 MS.gene55759:intron 30.0%
GTTGTATGCAATTGTAGTAT+CGG + chr4.3:5125740-5125759 MS.gene55759:CDS 30.0%
TGATTGTTCAACACTTGAAA+GGG - chr4.3:5125691-5125710 None:intergenic 30.0%
TTCCTCGCAGTTAAAATTTA+GGG + chr4.3:5125230-5125249 MS.gene55759:intron 30.0%
TTGATTGTTCAACACTTGAA+AGG - chr4.3:5125692-5125711 None:intergenic 30.0%
! GTTAAAATTTAGGGTACATG+TGG + chr4.3:5125239-5125258 MS.gene55759:intron 30.0%
!! TTGCACATTTTAACATACAG+AGG + chr4.3:5125874-5125893 MS.gene55759:CDS 30.0%
!!! CATTTTGGTTTATAGGATCT+TGG + chr4.3:5125376-5125395 MS.gene55759:intron 30.0%
ACCATCCCAAGAAATATAAG+TGG - chr4.3:5125906-5125925 None:intergenic 35.0%
AGACTATGCAACAACATTGA+TGG - chr4.3:5125966-5125985 None:intergenic 35.0%
ATATTTCTTGGGATGGTGAT+GGG + chr4.3:5125909-5125928 MS.gene55759:CDS 35.0%
ATCAGTGACAAGGCTAAATA+GGG - chr4.3:5125466-5125485 None:intergenic 35.0%
CAATGTTGTTGCATAGTCTT+TGG + chr4.3:5125967-5125986 MS.gene55759:CDS 35.0%
GATTTCAACTCTGCTCAATT+GGG - chr4.3:5125281-5125300 None:intergenic 35.0%
TATATTTCTTGGGATGGTGA+TGG + chr4.3:5125908-5125927 MS.gene55759:CDS 35.0%
TCATGTTTCCTCTTGTCAAT+CGG + chr4.3:5125613-5125632 MS.gene55759:CDS 35.0%
! ATGCTGCCACTTATATTTCT+TGG + chr4.3:5125897-5125916 MS.gene55759:CDS 35.0%
! TGATGGGTGTGATATTTACA+CGG + chr4.3:5125925-5125944 MS.gene55759:CDS 35.0%
! TGCTGCCACTTATATTTCTT+GGG + chr4.3:5125898-5125917 MS.gene55759:CDS 35.0%
! TTGTTGCATAGTCTTTGGAA+TGG + chr4.3:5125972-5125991 MS.gene55759:CDS 35.0%
!! ATGGCATTTTGATGTTGTCA+TGG + chr4.3:5125584-5125603 MS.gene55759:CDS 35.0%
!! TGGCATTTTGATGTTGTCAT+GGG + chr4.3:5125585-5125604 MS.gene55759:CDS 35.0%
!!! ACATCGGCATTTTGGTTTAT+AGG + chr4.3:5125369-5125388 MS.gene55759:intron 35.0%
!!! TACCCTAAATTTTAACTGCG+AGG - chr4.3:5125235-5125254 None:intergenic 35.0%
AAAACTATTTCCTCCCACAC+AGG - chr4.3:5125774-5125793 None:intergenic 40.0%
AACAATAAACGCTCAACCTG+AGG + chr4.3:5125304-5125323 MS.gene55759:intron 40.0%
AATAAAAGCAAGGGATCCTG+TGG + chr4.3:5125710-5125729 MS.gene55759:CDS 40.0%
AGGTTGAGCGTTTATTGTTC+AGG - chr4.3:5125303-5125322 None:intergenic 40.0%
AGTTTAGCCTGGAAGGAAAA+TGG + chr4.3:5125565-5125584 MS.gene55759:CDS 40.0%
CATCAGTGACAAGGCTAAAT+AGG - chr4.3:5125467-5125486 None:intergenic 40.0%
CTTCAGTTACATCAGTGACA+AGG - chr4.3:5125476-5125495 None:intergenic 40.0%
CTTGCTGAAGCCAGTTATAT+GGG - chr4.3:5125418-5125437 None:intergenic 40.0%
CTTGTCACTGATGTAACTGA+AGG + chr4.3:5125474-5125493 MS.gene55759:CDS 40.0%
GCATGTATTTGGTAAGTGAC+GGG - chr4.3:5125812-5125831 None:intergenic 40.0%
GCCACTTATATTTCTTGGGA+TGG + chr4.3:5125902-5125921 MS.gene55759:CDS 40.0%
GGATTTCAACTCTGCTCAAT+TGG - chr4.3:5125282-5125301 None:intergenic 40.0%
GGCATGTATTTGGTAAGTGA+CGG - chr4.3:5125813-5125832 None:intergenic 40.0%
GGTTATGCTTCTTTGGACAT+CGG + chr4.3:5125353-5125372 MS.gene55759:intron 40.0%
GTGTGATATTTACACGGTCA+AGG + chr4.3:5125931-5125950 MS.gene55759:CDS 40.0%
TTACCAAATACATGCCTGTG+TGG + chr4.3:5125817-5125836 MS.gene55759:CDS 40.0%
TTCTTTGACATTTAGAGGGC+AGG + chr4.3:5125440-5125459 MS.gene55759:CDS 40.0%
! TTCTTTGGACATCGGCATTT+TGG + chr4.3:5125361-5125380 MS.gene55759:intron 40.0%
!!! CAAAATGCCATTTTCCTTCC+AGG - chr4.3:5125575-5125594 None:intergenic 40.0%
AATATGGTCGTTGCACTCCA+TGG - chr4.3:5125143-5125162 None:intergenic 45.0%
ACACCACACAGGCATGTATT+TGG - chr4.3:5125823-5125842 None:intergenic 45.0%
AGGCTAAACTTGAACCAGTC+GGG - chr4.3:5125555-5125574 None:intergenic 45.0%
ATTCGTGCAATCTTTGACGC+AGG + chr4.3:5125332-5125351 MS.gene55759:intron 45.0%
ATTGCACGAATTACAGCCTC+AGG - chr4.3:5125323-5125342 None:intergenic 45.0%
CAGGCTAAACTTGAACCAGT+CGG - chr4.3:5125556-5125575 None:intergenic 45.0%
CCTTGCTGAAGCCAGTTATA+TGG - chr4.3:5125419-5125438 None:intergenic 45.0%
GCATACAACAACATTGCCAC+AGG - chr4.3:5125729-5125748 None:intergenic 45.0%
TAGGGTACATGTGGCAATCA+TGG + chr4.3:5125248-5125267 MS.gene55759:intron 45.0%
TGACGCAGGTTATGCTTCTT+TGG + chr4.3:5125346-5125365 MS.gene55759:intron 45.0%
! CCAGTTATATGGGCTGCAAA+AGG - chr4.3:5125408-5125427 None:intergenic 45.0%
! CCATATAACTGGCTTCAGCA+AGG + chr4.3:5125416-5125435 MS.gene55759:CDS 45.0%
! CCTTTTGCAGCCCATATAAC+TGG + chr4.3:5125405-5125424 MS.gene55759:CDS 45.0%
! GATGTAACTGAAGGACTAGC+TGG + chr4.3:5125483-5125502 MS.gene55759:CDS 45.0%
! TGGGAGGAAATAGTTTTCCG+CGG + chr4.3:5125777-5125796 MS.gene55759:CDS 45.0%
! TGGTTCAAGTTTAGCCTGGA+AGG + chr4.3:5125558-5125577 MS.gene55759:CDS 45.0%
ACCAGTATTGCACACCACAC+AGG - chr4.3:5125834-5125853 None:intergenic 50.0%
CTAAACTTGAACCAGTCGGG+TGG - chr4.3:5125552-5125571 None:intergenic 50.0%
TGAGAGAGCCGATTGACAAG+AGG - chr4.3:5125624-5125643 None:intergenic 50.0%
! CGACTGGTTCAAGTTTAGCC+TGG + chr4.3:5125554-5125573 MS.gene55759:CDS 50.0%
ACTTGAACCAGTCGGGTGGA+AGG - chr4.3:5125548-5125567 None:intergenic 55.0%
CTTGAACCAGTCGGGTGGAA+GGG - chr4.3:5125547-5125566 None:intergenic 55.0%
GAAAGGGTGACCTGTGTCGA+TGG - chr4.3:5125675-5125694 None:intergenic 55.0%
GCAGGATGGGTAGTAGGTCA+AGG - chr4.3:5125653-5125672 None:intergenic 55.0%
GCCTGTGTGGTGTGCAATAC+TGG + chr4.3:5125830-5125849 MS.gene55759:CDS 55.0%
TCGATGGCAGGATGGGTAGT+AGG - chr4.3:5125659-5125678 None:intergenic 55.0%
TTGAACCAGTCGGGTGGAAG+GGG - chr4.3:5125546-5125565 None:intergenic 55.0%
! ACCTGTGTCGATGGCAGGAT+GGG - chr4.3:5125666-5125685 None:intergenic 55.0%
! GTGACGGGAGTAGAAAACCG+CGG - chr4.3:5125797-5125816 None:intergenic 55.0%
ACCCATCCTGCCATCGACAC+AGG + chr4.3:5125662-5125681 MS.gene55759:CDS 60.0%
ATCGGTCTGCGCTCCTGTGT+GGG + chr4.3:5125758-5125777 MS.gene55759:CDS 60.0%
TATCGGTCTGCGCTCCTGTG+TGG + chr4.3:5125757-5125776 MS.gene55759:CDS 60.0%
! GACCTGTGTCGATGGCAGGA+TGG - chr4.3:5125667-5125686 None:intergenic 60.0%
ACCAGTCGGGTGGAAGGGGA+CGG - chr4.3:5125542-5125561 None:intergenic 65.0%
! GGGTGACCTGTGTCGATGGC+AGG - chr4.3:5125671-5125690 None:intergenic 65.0%
GGTCTGCGCTCCTGTGTGGG+AGG + chr4.3:5125761-5125780 MS.gene55759:CDS 70.0%
TCCGTCCCCTTCCACCCGAC+TGG + chr4.3:5125538-5125557 MS.gene55759:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 5125130 5126022 5125130 ID=MS.gene55759
chr4.3 mRNA 5125130 5126022 5125130 ID=MS.gene55759.t1;Parent=MS.gene55759
chr4.3 exon 5125130 5125166 5125130 ID=MS.gene55759.t1.exon1;Parent=MS.gene55759.t1
chr4.3 CDS 5125130 5125166 5125130 ID=cds.MS.gene55759.t1;Parent=MS.gene55759.t1
chr4.3 exon 5125391 5126022 5125391 ID=MS.gene55759.t1.exon2;Parent=MS.gene55759.t1
chr4.3 CDS 5125391 5126022 5125391 ID=cds.MS.gene55759.t1;Parent=MS.gene55759.t1
Gene Sequence

>MS.gene55759

ATGGGTTGTACCATGGAGTGCAACGACCATATTAAAAGTAAGTGTTTCTCTAAAGTTTCATCAAAGTATAATTATGAAAAGTTTCGCTTTTGTTTATCAATTCCTCGCAGTTAAAATTTAGGGTACATGTGGCAATCATGGAATGTATCCCAATTGAGCAGAGTTGAAATCCTGAACAATAAACGCTCAACCTGAGGCTGTAATTCGTGCAATCTTTGACGCAGGTTATGCTTCTTTGGACATCGGCATTTTGGTTTATAGGATCTTGGATGATTCCTTTTGCAGCCCATATAACTGGCTTCAGCAAGGATTCTTTGACATTTAGAGGGCAGGCCCTATTTAGCCTTGTCACTGATGTAACTGAAGGACTAGCTGGAATTGCAATTCTTGTTCGTTGTCTTTCACGATTCCGTCCCCTTCCACCCGACTGGTTCAAGTTTAGCCTGGAAGGAAAATGGCATTTTGATGTTGTCATGGGTTGTCTCATGTTTCCTCTTGTCAATCGGCTCTCTCAGTTCAACCTTGACCTACTACCCATCCTGCCATCGACACAGGTCACCCTTTCAAGTGTTGAACAATCAATAAAAGCAAGGGATCCTGTGGCAATGTTGTTGTATGCAATTGTAGTATCGGTCTGCGCTCCTGTGTGGGAGGAAATAGTTTTCCGCGGTTTTCTACTCCCGTCACTTACCAAATACATGCCTGTGTGGTGTGCAATACTGGTAAGTTCAGTTGCGTTTGCGCTTGCACATTTTAACATACAGAGGATGCTGCCACTTATATTTCTTGGGATGGTGATGGGTGTGATATTTACACGGTCAAGGAATTTATTGCCATCAATGTTGTTGCATAGTCTTTGGAATGGCTTTGTCTTTTTAGATTTGATGAAATAG

Protein sequence

>MS.gene55759.t1

MGCTMECNDHIKRSWMIPFAAHITGFSKDSLTFRGQALFSLVTDVTEGLAGIAILVRCLSRFRPLPPDWFKFSLEGKWHFDVVMGCLMFPLVNRLSQFNLDLLPILPSTQVTLSSVEQSIKARDPVAMLLYAIVVSVCAPVWEEIVFRGFLLPSLTKYMPVWCAILVSSVAFALAHFNIQRMLPLIFLGMVMGVIFTRSRNLLPSMLLHSLWNGFVFLDLMK