AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60631


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60631.t1 MTR_1g099550 87.349 332 16 5 1 325 1 313 0.0 563
MS.gene60631.t1 MTR_8g099045 30.447 358 164 13 1 295 1 336 3.09e-30 118
MS.gene60631.t1 MTR_5g009870 40.444 225 93 12 104 300 159 370 5.09e-30 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60631.t1 AT5G52870 33.546 313 147 11 1 299 1 266 1.41e-33 125
MS.gene60631.t1 AT5G52870 33.546 313 147 11 1 299 1 266 1.95e-33 125
MS.gene60631.t1 AT1G64080 49.206 126 45 4 193 300 266 390 7.48e-22 95.5

Find 58 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGAAACTCGCTCTGTTT+TGG 0.159958 1.2:+69723388 None:intergenic
CTTTAAACTTCCTCAAGTTC+TGG 0.310344 1.2:-69723931 MS.gene60631:CDS
GAAACTTTGGTAGCAAAATT+CGG 0.358487 1.2:-69723430 MS.gene60631:CDS
GATTAAGCCTCTTTATGTTA+AGG 0.375290 1.2:-69723339 MS.gene60631:CDS
TTTGTTGTCGAAATCATTCA+TGG 0.384507 1.2:+69723808 None:intergenic
TTCGAAACCTTAACATAAAG+AGG 0.384723 1.2:+69723332 None:intergenic
CACCGGCGGTTGGCTCTTTG+AGG 0.387789 1.2:-69723244 MS.gene60631:CDS
GAAAGTGGTGACGAAGCATT+TGG 0.391796 1.2:-69723189 MS.gene60631:CDS
TTGGACATGTGGGTCGTCAT+TGG 0.416385 1.2:+69723784 None:intergenic
ATGGATGTTGTTGGTGGTGA+TGG 0.423562 1.2:-69722903 MS.gene60631:CDS
GAGCTTGTTCTCCGGCGAAC+AGG 0.426734 1.2:-69723136 MS.gene60631:CDS
TTCAGAAAACAGAGAAGAAC+TGG 0.432309 1.2:-69723572 MS.gene60631:CDS
GGTAGCAAAATTCGGAATCA+AGG 0.437082 1.2:-69723422 MS.gene60631:CDS
GATTCTTCAATGGATGTTGT+TGG 0.446904 1.2:-69722912 MS.gene60631:CDS
AGGTCGTCGGGTACGGTGGC+CGG 0.454518 1.2:-69723158 MS.gene60631:CDS
GAGAACAGCACAAGAAACTT+TGG 0.457374 1.2:-69723443 MS.gene60631:CDS
TGCTGATCTTAGAAGTGAAA+TGG 0.459585 1.2:+69723616 None:intergenic
TTCGAAGAGGTACGGTGATA+AGG 0.465764 1.2:-69723315 MS.gene60631:CDS
TGCTTCGCCGTCGTCGTCAC+CGG 0.472585 1.2:-69723261 MS.gene60631:CDS
TAAGAGTAGGTCGTCGGGTA+CGG 0.475412 1.2:-69723165 MS.gene60631:CDS
GGTGATAAGGTGAAATTCTC+CGG 0.507388 1.2:-69723302 MS.gene60631:CDS
CTGTTCTCTCTACTAAGCAT+TGG 0.523196 1.2:+69723458 None:intergenic
GTCACCACTTTCATCCCAGC+CGG 0.525414 1.2:+69723200 None:intergenic
AAAGTGGTGACGAAGCATTT+GGG 0.526267 1.2:-69723188 MS.gene60631:CDS
AAGATGAGGAACAGTGATGT+TGG 0.527025 1.2:+69723889 None:intergenic
GAGGAAGTTTAAAGCATCCA+TGG 0.529668 1.2:+69723940 None:intergenic
AGCTACCGTCATCCCCTCGC+CGG 0.534216 1.2:+69723283 None:intergenic
CAAAACAGAGCGAGTTTCAA+AGG 0.536455 1.2:-69723387 MS.gene60631:CDS
TGTTGTTCTTCCAGAACTTG+AGG 0.537675 1.2:+69723921 None:intergenic
GTTAAGGTTTCGAAGAGGTA+CGG 0.540829 1.2:-69723323 MS.gene60631:CDS
TTGGGTAAGAGTAGGTCGTC+GGG 0.544095 1.2:-69723170 MS.gene60631:CDS
CCGTCGTCGTCACCGGCGGT+TGG 0.545238 1.2:-69723254 MS.gene60631:CDS
AAGGTGAAATTCTCCGGCGA+GGG 0.550532 1.2:-69723296 MS.gene60631:CDS
AAGGTAAAGATTCTGAAACT+TGG 0.555444 1.2:+69723593 None:intergenic
TTCCTCAAAGAGCCAACCGC+CGG 0.558224 1.2:+69723242 None:intergenic
AGAAGTGAAATGGGTGATTG+TGG 0.566819 1.2:+69723626 None:intergenic
GGTGGCCGGAGCTTGTTCTC+CGG 0.566890 1.2:-69723144 MS.gene60631:CDS
ATTCTCCGGCGAGGGGATGA+CGG 0.568736 1.2:-69723288 MS.gene60631:CDS
GCTGATCTTAGAAGTGAAAT+GGG 0.571175 1.2:+69723617 None:intergenic
CGAAGCATTTGGGTAAGAGT+AGG 0.585768 1.2:-69723178 MS.gene60631:CDS
TTTGGGTAAGAGTAGGTCGT+CGG 0.592489 1.2:-69723171 MS.gene60631:CDS
ACGCTGTTGCAGCAACATGA+TGG 0.598349 1.2:-69723104 MS.gene60631:CDS
GTATCATCACTCCTGTTCGC+CGG 0.598908 1.2:+69723125 None:intergenic
TCTTCAATGGATGTTGTTGG+TGG 0.599000 1.2:-69722909 MS.gene60631:CDS
GTTCGCCGGAGAACAAGCTC+CGG 0.605965 1.2:+69723139 None:intergenic
TTCGCCGTCGTCGTCACCGG+CGG 0.615008 1.2:-69723258 MS.gene60631:CDS
GAGTAGGTCGTCGGGTACGG+TGG 0.623063 1.2:-69723162 MS.gene60631:CDS
CCAACCGCCGGTGACGACGA+CGG 0.626873 1.2:+69723254 None:intergenic
CTTTGAGGAAAGAGAAACAC+GGG 0.634887 1.2:-69723229 MS.gene60631:CDS
TCTGTTTCTACAACAAGATG+AGG 0.645146 1.2:+69723875 None:intergenic
AAGAGATCCTTCAATTCCAG+AGG 0.646564 1.2:-69723056 MS.gene60631:intron
AGGTGAAATTCTCCGGCGAG+GGG 0.653189 1.2:-69723295 MS.gene60631:CDS
GAATCAGAAGAAAGAAACCA+AGG 0.653413 1.2:-69723525 MS.gene60631:CDS
TAAGGTGAAATTCTCCGGCG+AGG 0.664840 1.2:-69723297 MS.gene60631:CDS
ATGAGGAACAGTGATGTTGG+AGG 0.666650 1.2:+69723892 None:intergenic
TTTATGTTAAGGTTTCGAAG+AGG 0.679040 1.2:-69723328 MS.gene60631:CDS
AATTCGGAATCAAGGAACAG+AGG 0.681760 1.2:-69723414 MS.gene60631:CDS
TCTTTGAGGAAAGAGAAACA+CGG 0.735127 1.2:-69723230 MS.gene60631:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATATGATTATTTTTGAA+AGG - chr1.2:69723806-69723825 MS.gene60631:CDS 10.0%
!!! ATAATTTTCAGATTCTTCAA+TGG - chr1.2:69723876-69723895 MS.gene60631:CDS 20.0%
!! TTCAACAAGAAATTCTTTTG+AGG - chr1.2:69723927-69723946 MS.gene60631:CDS 25.0%
!!! ATAAGAAGTGTTGTTTTTTG+AGG + chr1.2:69723094-69723113 None:intergenic 25.0%
GATTAAGCCTCTTTATGTTA+AGG - chr1.2:69723459-69723478 MS.gene60631:CDS 30.0%
TAGTTTCACAGAAAAAACCT+TGG + chr1.2:69723293-69723312 None:intergenic 30.0%
TTCGAAACCTTAACATAAAG+AGG + chr1.2:69723469-69723488 None:intergenic 30.0%
TTTATGTTAAGGTTTCGAAG+AGG - chr1.2:69723470-69723489 MS.gene60631:CDS 30.0%
! GAAACTTTGGTAGCAAAATT+CGG - chr1.2:69723368-69723387 MS.gene60631:CDS 30.0%
! GTTTTCTGTTTTGTGAGTTT+TGG + chr1.2:69723041-69723060 None:intergenic 30.0%
! TGTGAAACAAATTTTACCTC+TGG + chr1.2:69723761-69723780 None:intergenic 30.0%
! TTTGTTGTCGAAATCATTCA+TGG + chr1.2:69722993-69723012 None:intergenic 30.0%
!! AAGGTAAAGATTCTGAAACT+TGG + chr1.2:69723208-69723227 None:intergenic 30.0%
!! ATTAGTTTCAGTTTTCTCGT+AGG + chr1.2:69723068-69723087 None:intergenic 30.0%
!!! AATTTTACCTCTGGAATTGA+AGG + chr1.2:69723752-69723771 None:intergenic 30.0%
!!! AGCACTTTTCTTTTTGAGAT+AGG + chr1.2:69723130-69723149 None:intergenic 30.0%
CTTTAAACTTCCTCAAGTTC+TGG - chr1.2:69722867-69722886 MS.gene60631:CDS 35.0%
GAATCAGAAGAAAGAAACCA+AGG - chr1.2:69723273-69723292 MS.gene60631:CDS 35.0%
GATTCTTCAATGGATGTTGT+TGG - chr1.2:69723886-69723905 MS.gene60631:CDS 35.0%
GCTGATCTTAGAAGTGAAAT+GGG + chr1.2:69723184-69723203 None:intergenic 35.0%
TGCTGATCTTAGAAGTGAAA+TGG + chr1.2:69723185-69723204 None:intergenic 35.0%
TTCAGAAAACAGAGAAGAAC+TGG - chr1.2:69723226-69723245 MS.gene60631:CDS 35.0%
! AGTTCTTCTCTGTTTTCTGA+AGG + chr1.2:69723227-69723246 None:intergenic 35.0%
! TCTTTGAGGAAAGAGAAACA+CGG - chr1.2:69723568-69723587 MS.gene60631:CDS 35.0%
!! TCTGTTTCTACAACAAGATG+AGG + chr1.2:69722926-69722945 None:intergenic 35.0%
!! TTTGAAACTCGCTCTGTTTT+GGG + chr1.2:69723412-69723431 None:intergenic 35.0%
!!! CTGTTTTGTGAGTTTTGGTT+TGG + chr1.2:69723036-69723055 None:intergenic 35.0%
!!! CTTTTTGAGATAGGTTCGTT+TGG + chr1.2:69723121-69723140 None:intergenic 35.0%
!!! GGTGATTGTGGTTTTGAAAT+GGG + chr1.2:69723163-69723182 None:intergenic 35.0%
!!! GGTTTTGAAATGGGTTCAAT+TGG + chr1.2:69723154-69723173 None:intergenic 35.0%
AAGAGATCCTTCAATTCCAG+AGG - chr1.2:69723742-69723761 MS.gene60631:CDS 40.0%
AAGATGAGGAACAGTGATGT+TGG + chr1.2:69722912-69722931 None:intergenic 40.0%
AATTCGGAATCAAGGAACAG+AGG - chr1.2:69723384-69723403 MS.gene60631:CDS 40.0%
AGAAGTGAAATGGGTGATTG+TGG + chr1.2:69723175-69723194 None:intergenic 40.0%
CAAAACAGAGCGAGTTTCAA+AGG - chr1.2:69723411-69723430 MS.gene60631:CDS 40.0%
CTGTTCTCTCTACTAAGCAT+TGG + chr1.2:69723343-69723362 None:intergenic 40.0%
GAGAACAGCACAAGAAACTT+TGG - chr1.2:69723355-69723374 MS.gene60631:CDS 40.0%
GGTAGCAAAATTCGGAATCA+AGG - chr1.2:69723376-69723395 MS.gene60631:CDS 40.0%
GGTGATAAGGTGAAATTCTC+CGG - chr1.2:69723496-69723515 MS.gene60631:CDS 40.0%
GTTAAGGTTTCGAAGAGGTA+CGG - chr1.2:69723475-69723494 MS.gene60631:CDS 40.0%
TCTTCAATGGATGTTGTTGG+TGG - chr1.2:69723889-69723908 MS.gene60631:CDS 40.0%
TGTTGTTCTTCCAGAACTTG+AGG + chr1.2:69722880-69722899 None:intergenic 40.0%
! AAAGTGGTGACGAAGCATTT+GGG - chr1.2:69723610-69723629 MS.gene60631:CDS 40.0%
! CTTTGAGGAAAGAGAAACAC+GGG - chr1.2:69723569-69723588 MS.gene60631:CDS 40.0%
!!! CTTTGAAACTCGCTCTGTTT+TGG + chr1.2:69723413-69723432 None:intergenic 40.0%
!!! GAGTTTTGGTTTGGACATGT+GGG + chr1.2:69723027-69723046 None:intergenic 40.0%
!!! GGGTGATTGTGGTTTTGAAA+TGG + chr1.2:69723164-69723183 None:intergenic 40.0%
!!! TGAGTTTTGGTTTGGACATG+TGG + chr1.2:69723028-69723047 None:intergenic 40.0%
ATGAGGAACAGTGATGTTGG+AGG + chr1.2:69722909-69722928 None:intergenic 45.0%
TTTGGGTAAGAGTAGGTCGT+CGG - chr1.2:69723627-69723646 MS.gene60631:CDS 45.0%
! AGAGAAACACGGGAGTTTTC+CGG - chr1.2:69723579-69723598 MS.gene60631:CDS 45.0%
! CGAAGCATTTGGGTAAGAGT+AGG - chr1.2:69723620-69723639 MS.gene60631:CDS 45.0%
! GAAAGTGGTGACGAAGCATT+TGG - chr1.2:69723609-69723628 MS.gene60631:CDS 45.0%
! TTCGAAGAGGTACGGTGATA+AGG - chr1.2:69723483-69723502 MS.gene60631:CDS 45.0%
!! ATGGATGTTGTTGGTGGTGA+TGG - chr1.2:69723895-69723914 MS.gene60631:CDS 45.0%
AAGGTGAAATTCTCCGGCGA+GGG - chr1.2:69723502-69723521 MS.gene60631:CDS 50.0%
ACGCTGTTGCAGCAACATGA+TGG - chr1.2:69723694-69723713 MS.gene60631:CDS 50.0%
GTATCATCACTCCTGTTCGC+CGG + chr1.2:69723676-69723695 None:intergenic 50.0%
TAAGAGTAGGTCGTCGGGTA+CGG - chr1.2:69723633-69723652 MS.gene60631:CDS 50.0%
TAAGGTGAAATTCTCCGGCG+AGG - chr1.2:69723501-69723520 MS.gene60631:CDS 50.0%
TTGGACATGTGGGTCGTCAT+TGG + chr1.2:69723017-69723036 None:intergenic 50.0%
TTGGGTAAGAGTAGGTCGTC+GGG - chr1.2:69723628-69723647 MS.gene60631:CDS 50.0%
! TTTTCCGGCTGGGATGAAAG+TGG - chr1.2:69723594-69723613 MS.gene60631:CDS 50.0%
AGGTGAAATTCTCCGGCGAG+GGG - chr1.2:69723503-69723522 MS.gene60631:CDS 55.0%
GTCACCACTTTCATCCCAGC+CGG + chr1.2:69723601-69723620 None:intergenic 55.0%
TTCCTCAAAGAGCCAACCGC+CGG + chr1.2:69723559-69723578 None:intergenic 55.0%
! AAACACGGGAGTTTTCCGGC+TGG - chr1.2:69723583-69723602 MS.gene60631:CDS 55.0%
! AACACGGGAGTTTTCCGGCT+GGG - chr1.2:69723584-69723603 MS.gene60631:CDS 55.0%
ATTCTCCGGCGAGGGGATGA+CGG - chr1.2:69723510-69723529 MS.gene60631:CDS 60.0%
GTTCGCCGGAGAACAAGCTC+CGG + chr1.2:69723662-69723681 None:intergenic 60.0%
! GAGCTTGTTCTCCGGCGAAC+AGG - chr1.2:69723662-69723681 MS.gene60631:CDS 60.0%
AGCTACCGTCATCCCCTCGC+CGG + chr1.2:69723518-69723537 None:intergenic 65.0%
GAGTAGGTCGTCGGGTACGG+TGG - chr1.2:69723636-69723655 MS.gene60631:CDS 65.0%
TGCTTCGCCGTCGTCGTCAC+CGG - chr1.2:69723537-69723556 MS.gene60631:CDS 65.0%
! CACCGGCGGTTGGCTCTTTG+AGG - chr1.2:69723554-69723573 MS.gene60631:CDS 65.0%
! GGTGGCCGGAGCTTGTTCTC+CGG - chr1.2:69723654-69723673 MS.gene60631:CDS 65.0%
AGGTCGTCGGGTACGGTGGC+CGG - chr1.2:69723640-69723659 MS.gene60631:CDS 70.0%
CCAACCGCCGGTGACGACGA+CGG + chr1.2:69723547-69723566 None:intergenic 70.0%
TTCGCCGTCGTCGTCACCGG+CGG - chr1.2:69723540-69723559 MS.gene60631:CDS 70.0%
CCGTCGTCGTCACCGGCGGT+TGG - chr1.2:69723544-69723563 MS.gene60631:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 69722860 69723960 69722860 ID=MS.gene60631
chr1.2 mRNA 69722860 69723960 69722860 ID=MS.gene60631.t1;Parent=MS.gene60631
chr1.2 exon 69723057 69723960 69723057 ID=MS.gene60631.t1.exon1;Parent=MS.gene60631.t1
chr1.2 CDS 69723057 69723960 69723057 ID=cds.MS.gene60631.t1;Parent=MS.gene60631.t1
chr1.2 exon 69722860 69722933 69722860 ID=MS.gene60631.t1.exon2;Parent=MS.gene60631.t1
chr1.2 CDS 69722860 69722933 69722860 ID=cds.MS.gene60631.t1;Parent=MS.gene60631.t1
Gene Sequence

>MS.gene60631

ATGGATGCTTTAAACTTCCTCAAGTTCTGGAAGAACAACACAACAACCTCCAACATCACTGTTCCTCATCTTGTTGTAGAAACAGACAGCGAATCAGACGAAGATGACTCATTCTTCGACTTAGAACTCTCCATGAATGATTTCGACAACAAAACCAATGACGACCCACATGTCCAAACCAAAACTCACAAAACAGAAAACATTACCTACGAGAAAACTGAAACTAATGTTCCTCAAAAAACAACACTTCTTATGTCTCCAAACGAACCTATCTCAAAAAGAAAAGTGCTTCCAATTGAACCCATTTCAAAACCACAATCACCCATTTCACTTCTAAGATCAGCACCAAGTTTCAGAATCTTTACCTTCAGAAAACAGAGAAGAACTGGTTCTGAAAAAACAGAGTGTGAAAAGAATCAGAAGAAAGAAACCAAGGTTTTTTCTGTGAAACTAAATATAGAAGAAGATTTTCATTCTACTCCAATGCTTAGTAGAGAGAACAGCACAAGAAACTTTGGTAGCAAAATTCGGAATCAAGGAACAGAGGAACCCAAAACAGAGCGAGTTTCAAAGGAGATTTTGCAGAAATATTTGAAACTGATTAAGCCTCTTTATGTTAAGGTTTCGAAGAGGTACGGTGATAAGGTGAAATTCTCCGGCGAGGGGATGACGGTAGCTGCTTCGCCGTCGTCGTCACCGGCGGTTGGCTCTTTGAGGAAAGAGAAACACGGGAGTTTTCCGGCTGGGATGAAAGTGGTGACGAAGCATTTGGGTAAGAGTAGGTCGTCGGGTACGGTGGCCGGAGCTTGTTCTCCGGCGAACAGGAGTGATGATACGCTGTTGCAGCAACATGATGGAATTCAAAGTGCTATTCTTCATTGCAAGAGATCCTTCAATTCCAGAGGTAAAATTTGTTTCACAAATAATATTTTTTTTATTCATATGCTAAATATGATTATTTTTGAAAGGATATGCTAAATATGATTTGTAATGTCAATTAATTATAGTTAATTTTTATAATTTTCAGATTCTTCAATGGATGTTGTTGGTGGTGATGGATCAATGTATTCAACAAGAAATTCTTTTGAGGATGAAGTGTGA

Protein sequence

>MS.gene60631.t1

MDALNFLKFWKNNTTTSNITVPHLVVETDSESDEDDSFFDLELSMNDFDNKTNDDPHVQTKTHKTENITYEKTETNVPQKTTLLMSPNEPISKRKVLPIEPISKPQSPISLLRSAPSFRIFTFRKQRRTGSEKTECEKNQKKETKVFSVKLNIEEDFHSTPMLSRENSTRNFGSKIRNQGTEEPKTERVSKEILQKYLKLIKPLYVKVSKRYGDKVKFSGEGMTVAASPSSSPAVGSLRKEKHGSFPAGMKVVTKHLGKSRSSGTVAGACSPANRSDDTLLQQHDGIQSAILHCKRSFNSRDSSMDVVGGDGSMYSTRNSFEDEV