AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61130.t1 MTR_4g063060 98.693 153 1 1 1 153 1 152 1.17e-103 293
MS.gene61130.t1 MTR_2g096340 96.689 151 5 0 3 153 4 154 4.98e-101 286
MS.gene61130.t1 MTR_2g096340 96.644 149 5 0 5 153 13 161 2.55e-99 282
MS.gene61130.t1 MTR_7g023260 92.053 151 12 0 3 153 4 154 1.65e-67 202
MS.gene61130.t1 MTR_4g007840 94.595 37 2 0 117 153 36 72 1.95e-18 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61130.t1 AT3G55280 87.662 154 18 1 1 153 1 154 2.30e-70 209
MS.gene61130.t1 AT3G55280 87.662 154 18 1 1 153 1 154 2.30e-70 209
MS.gene61130.t1 AT2G39460 89.844 128 13 0 26 153 27 154 1.19e-66 199
MS.gene61130.t1 AT2G39460 89.844 128 13 0 26 153 27 154 1.19e-66 199
MS.gene61130.t1 AT3G55280 92.373 118 9 0 36 153 31 148 2.54e-66 199

Find 43 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCCCTTGTTTGTGTCTT+TGG 0.162619 4.4:+42795340 None:intergenic
GCTTTCAATGCCTGTGCCTT+TGG 0.265621 4.4:+42795439 None:intergenic
GGCACTGATGCGAGGATATT+TGG 0.276630 4.4:+42795312 None:intergenic
TATCCCTTGTTTGTGTCTTT+GGG 0.296408 4.4:+42795341 None:intergenic
CAACCTAACATAGGCCTTCT+TGG 0.308307 4.4:+42794604 None:intergenic
GCACTGATGCGAGGATATTT+GGG 0.324549 4.4:+42795313 None:intergenic
TAGTGGTCAAGCTTGTTTCT+TGG 0.344862 4.4:+42795286 None:intergenic
TTGGATGTAGCAAACAAGAT+TGG 0.425365 4.4:-42794563 MS.gene61130:CDS
ACATCCAATGCATCGTAATC+AGG 0.425991 4.4:+42794578 None:intergenic
CAATGCCTGTGCCTTTGGAT+CGG 0.450132 4.4:+42795444 None:intergenic
AGGTGTTAACCTTCTTCGCC+TGG 0.454264 4.4:+42795128 None:intergenic
TGGATGTAGCAAACAAGATT+GGG 0.476041 4.4:-42794562 MS.gene61130:CDS
GCTGCCAAGGCAGTCAAGTC+AGG 0.480557 4.4:-42795418 MS.gene61130:CDS
GTCAAGCTTGTTTCTTGGTG+TGG 0.490928 4.4:+42795291 None:intergenic
GTACCAAGAAGGCCTATGTT+AGG 0.503933 4.4:-42794607 MS.gene61130:CDS
CATAAATGCTCACCTGATCA+AGG 0.508831 4.4:+42795108 None:intergenic
GGAACTTCAAAATCTGATAG+TGG 0.511992 4.4:+42795269 None:intergenic
TTGTTTGTGTCTTTGGGCGA+TGG 0.513342 4.4:+42795347 None:intergenic
AATTGAGGATAACAACACTT+TGG 0.518687 4.4:-42795218 MS.gene61130:CDS
ATAGGCCTTCTTGGTACCAT+CGG 0.519310 4.4:+42794613 None:intergenic
GAAGAAAATGTATGACATCC+AGG 0.526017 4.4:-42795146 MS.gene61130:CDS
AGAAGGTTAACACCTTGATC+AGG 0.531796 4.4:-42795120 MS.gene61130:intron
GACACCTGATTACGATGCAT+TGG 0.535533 4.4:-42794582 MS.gene61130:CDS
AGTGCCTGACTTGACTGCCT+TGG 0.543420 4.4:+42795414 None:intergenic
TAGGCCTTCTTGGTACCATC+GGG 0.543529 4.4:+42794614 None:intergenic
CAAAGACACAAACAAGGGAT+AGG 0.544147 4.4:-42795339 MS.gene61130:CDS
CAGGAATCAATGGCTCCAAA+AGG 0.548086 4.4:-42796545 None:intergenic
AGGCACTGCTATTAAGAAGA+AGG 0.555789 4.4:-42795398 MS.gene61130:CDS
GTATGACATCCAGGCGAAGA+AGG 0.560881 4.4:-42795137 MS.gene61130:CDS
TTAATCTGTTGCAGGCCCGA+TGG 0.562967 4.4:-42794629 MS.gene61130:intron
ACAGGCATTGAAAGCTGCCA+AGG 0.571400 4.4:-42795431 MS.gene61130:CDS
GAAGACCGATCCAAAGGCAC+AGG 0.572296 4.4:-42795449 MS.gene61130:CDS
GTCCGCTATGAAGAAAATTG+AGG 0.584569 4.4:-42795233 MS.gene61130:CDS
CAGGCCCGATGGTACCAAGA+AGG 0.589470 4.4:-42794618 MS.gene61130:CDS
TAGCGGACTCAGTGGTGAGT+GGG 0.605079 4.4:+42795248 None:intergenic
TTCTAAGAAGACCGATCCAA+AGG 0.610830 4.4:-42795455 MS.gene61130:CDS
CACTGATGCGAGGATATTTG+GGG 0.617729 4.4:+42795314 None:intergenic
CTTGGTGTGGCACTGATGCG+AGG 0.626082 4.4:+42795304 None:intergenic
ATAGCGGACTCAGTGGTGAG+TGG 0.634672 4.4:+42795247 None:intergenic
ATCAGGTGTCAACCTAACAT+AGG 0.641379 4.4:+42794595 None:intergenic
ATCGCCCAAAGACACAAACA+AGG 0.648974 4.4:-42795345 MS.gene61130:CDS
TTTCTTCATAGCGGACTCAG+TGG 0.651024 4.4:+42795240 None:intergenic
TCGCCCAAAGACACAAACAA+GGG 0.727590 4.4:-42795344 MS.gene61130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAATCAAATAATGCA+TGG - chr4.4:42795512-42795531 MS.gene61130:intron 15.0%
!! AATCAAATCATACATGAAAA+TGG + chr4.4:42794748-42794767 None:intergenic 20.0%
!! AGCTTATGATATAACATTAA+GGG + chr4.4:42795400-42795419 None:intergenic 20.0%
!! CTGAAGAACTTATAAAATTA+AGG - chr4.4:42794953-42794972 MS.gene61130:intron 20.0%
!! TTAATTATTATAGTTCAGCA+TGG - chr4.4:42796407-42796426 MS.gene61130:intron 20.0%
!!! CTTAATTTTATAAGTTCTTC+AGG + chr4.4:42794955-42794974 None:intergenic 20.0%
!!! CTTACATAAGCTATTTTTAT+AGG - chr4.4:42795103-42795122 MS.gene61130:intron 20.0%
!!! TTCATGTATGATTTGATTTT+AGG - chr4.4:42794750-42794769 MS.gene61130:intron 20.0%
!!! TTTTCACTAACAAAAATGAA+TGG + chr4.4:42794636-42794655 None:intergenic 20.0%
! ATCAAATAATGCATGGAATT+AGG - chr4.4:42795519-42795538 MS.gene61130:intron 25.0%
! ATGATGAGCAAAAAAAAGTT+AGG - chr4.4:42794808-42794827 MS.gene61130:intron 25.0%
! ATTAAGGTGAAATTAGTGTA+TGG - chr4.4:42794969-42794988 MS.gene61130:intron 25.0%
! CAGCTTATGATATAACATTA+AGG + chr4.4:42795401-42795420 None:intergenic 25.0%
! GATTTACAAATTTGAGCTTA+TGG + chr4.4:42795013-42795032 None:intergenic 25.0%
! TACAAATTATCTCAAATGTC+AGG + chr4.4:42796115-42796134 None:intergenic 25.0%
! TGCTGATAAGAAAAAGATTA+AGG - chr4.4:42795913-42795932 MS.gene61130:intron 25.0%
!! AGAAAATTGAAGTTACCTTT+TGG + chr4.4:42794562-42794581 None:intergenic 25.0%
!! GATTAATACTGATTATCCAT+AGG - chr4.4:42795475-42795494 MS.gene61130:intron 25.0%
!! TTCTAGTTGAATATGTGTTT+AGG - chr4.4:42796245-42796264 MS.gene61130:intron 25.0%
AATAAGTTGCTTAACCAAAC+AGG + chr4.4:42795053-42795072 None:intergenic 30.0%
AATTGAGGATAACAACACTT+TGG - chr4.4:42795871-42795890 MS.gene61130:intron 30.0%
AGAAAACAGAAAACCATTGT+TGG + chr4.4:42796229-42796248 None:intergenic 30.0%
AGCACATAAACTTAAATCAG+TGG + chr4.4:42794599-42794618 None:intergenic 30.0%
ATGTCTGCAAAATATACTAC+TGG + chr4.4:42796140-42796159 None:intergenic 30.0%
ATTTATGCAGTTACTGTTCA+AGG - chr4.4:42795997-42796016 MS.gene61130:intron 30.0%
TTAGGCATTAATACCAACAA+TGG - chr4.4:42796213-42796232 MS.gene61130:intron 30.0%
! ATCCTCAATTTTCTTCATAG+CGG + chr4.4:42795861-42795880 None:intergenic 30.0%
! GAATCTGGAATTAATGATCA+GGG - chr4.4:42796355-42796374 MS.gene61130:intron 30.0%
! TTCTAGTTGAATACGTGTTT+AGG - chr4.4:42796195-42796214 MS.gene61130:intron 30.0%
!!! TTTTTTGCTCATCATAACTC+AGG + chr4.4:42794803-42794822 None:intergenic 30.0%
AACTATCCTTACATCCTGTT+TGG - chr4.4:42795036-42795055 MS.gene61130:intron 35.0%
CTAGAAAACAGAACCATTGT+TGG + chr4.4:42796279-42796298 None:intergenic 35.0%
GAAGAAAATGTATGACATCC+AGG - chr4.4:42795943-42795962 MS.gene61130:intron 35.0%
GGAACTTCAAAATCTGATAG+TGG + chr4.4:42795823-42795842 None:intergenic 35.0%
TATAACATTAAGGGCCTGTT+TGG + chr4.4:42795391-42795410 None:intergenic 35.0%
TATACTGAACCAAAAGCCTA+TGG + chr4.4:42795494-42795513 None:intergenic 35.0%
TCAAATAAGCCAATCCAAAC+AGG - chr4.4:42795374-42795393 MS.gene61130:CDS 35.0%
TGGATGTAGCAAACAAGATT+GGG - chr4.4:42796527-42796546 MS.gene61130:intron 35.0%
TTGGATGTAGCAAACAAGAT+TGG - chr4.4:42796526-42796545 MS.gene61130:intron 35.0%
! ACAGGATGTAAGGATAGTTT+TGG + chr4.4:42795035-42795054 None:intergenic 35.0%
! ACTGATTATCCATAGGCTTT+TGG - chr4.4:42795482-42795501 MS.gene61130:intron 35.0%
! GGAATCTGGAATTAATGATC+AGG - chr4.4:42796354-42796373 MS.gene61130:intron 35.0%
! TATCCCTTGTTTGTGTCTTT+GGG + chr4.4:42795751-42795770 None:intergenic 35.0%
! TCGACTTTTTAATCTGTTGC+AGG - chr4.4:42796452-42796471 MS.gene61130:intron 35.0%
!! AGACATACCGAACCATTTTT+GGG - chr4.4:42796154-42796173 MS.gene61130:intron 35.0%
!! CTTATGCAATGTTTTTGCAC+AGG - chr4.4:42795605-42795624 MS.gene61130:intron 35.0%
AAACCGCCAAAACCCAAAAA+TGG + chr4.4:42796169-42796188 None:intergenic 40.0%
ACATCCAATGCATCGTAATC+AGG + chr4.4:42796514-42796533 None:intergenic 40.0%
ACCCTAACAAAACATGCGAA+GGG + chr4.4:42796088-42796107 None:intergenic 40.0%
AGAAGGTTAACACCTTGATC+AGG - chr4.4:42795969-42795988 MS.gene61130:intron 40.0%
AGTTCAGTGCAATGCTATCA+GGG - chr4.4:42796329-42796348 MS.gene61130:intron 40.0%
ATACGTTGTGTGAGACTGTT+TGG + chr4.4:42795340-42795359 None:intergenic 40.0%
ATAGCATTGCACTGAACTGT+TGG + chr4.4:42796327-42796346 None:intergenic 40.0%
ATCAGGTGTCAACCTAACAT+AGG + chr4.4:42796497-42796516 None:intergenic 40.0%
CAAAGACACAAACAAGGGAT+AGG - chr4.4:42795750-42795769 MS.gene61130:intron 40.0%
CATAAATGCTCACCTGATCA+AGG + chr4.4:42795984-42796003 None:intergenic 40.0%
GAGTTTGTAAGCGACTGTTT+AGG - chr4.4:42794772-42794791 MS.gene61130:intron 40.0%
GCCAAAACCCAAAAATGGTT+CGG + chr4.4:42796164-42796183 None:intergenic 40.0%
GCTTAACCAAACAGGATGTA+AGG + chr4.4:42795045-42795064 None:intergenic 40.0%
GTCCGCTATGAAGAAAATTG+AGG - chr4.4:42795856-42795875 MS.gene61130:intron 40.0%
TACGTTGTGTGAGACTGTTT+GGG + chr4.4:42795339-42795358 None:intergenic 40.0%
! AGGCACTGCTATTAAGAAGA+AGG - chr4.4:42795691-42795710 MS.gene61130:intron 40.0%
! CAGACATACCGAACCATTTT+TGG - chr4.4:42796153-42796172 MS.gene61130:intron 40.0%
! CTATCCCTTGTTTGTGTCTT+TGG + chr4.4:42795752-42795771 None:intergenic 40.0%
! TAGTGGTCAAGCTTGTTTCT+TGG + chr4.4:42795806-42795825 None:intergenic 40.0%
! TTCTAAGAAGACCGATCCAA+AGG - chr4.4:42795634-42795653 MS.gene61130:intron 40.0%
!! ACCCTTCGCATGTTTTGTTA+GGG - chr4.4:42796084-42796103 MS.gene61130:intron 40.0%
!! TACCCTTCGCATGTTTTGTT+AGG - chr4.4:42796083-42796102 MS.gene61130:intron 40.0%
!!! ACCGAACCATTTTTGGGTTT+TGG - chr4.4:42796160-42796179 MS.gene61130:intron 40.0%
!!! GAACCATTTTTGGGTTTTGG+CGG - chr4.4:42796163-42796182 MS.gene61130:intron 40.0%
ATAGGCCTTCTTGGTACCAT+CGG + chr4.4:42796479-42796498 None:intergenic 45.0%
ATCGCCCAAAGACACAAACA+AGG - chr4.4:42795744-42795763 MS.gene61130:intron 45.0%
ATGCTATCAGGGTTGGAATC+TGG - chr4.4:42796340-42796359 MS.gene61130:intron 45.0%
CAACCTAACATAGGCCTTCT+TGG + chr4.4:42796488-42796507 None:intergenic 45.0%
CACCCTAACAAAACATGCGA+AGG + chr4.4:42796089-42796108 None:intergenic 45.0%
CACTGATGCGAGGATATTTG+GGG + chr4.4:42795778-42795797 None:intergenic 45.0%
CAGTTCAGTGCAATGCTATC+AGG - chr4.4:42796328-42796347 MS.gene61130:intron 45.0%
CATTAAGGGCCTGTTTGGAT+TGG + chr4.4:42795386-42795405 None:intergenic 45.0%
GACACCTGATTACGATGCAT+TGG - chr4.4:42796507-42796526 MS.gene61130:intron 45.0%
GTACCAAGAAGGCCTATGTT+AGG - chr4.4:42796482-42796501 MS.gene61130:intron 45.0%
TCGCCCAAAGACACAAACAA+GGG - chr4.4:42795745-42795764 MS.gene61130:intron 45.0%
TTGTTTGTGTCTTTGGGCGA+TGG + chr4.4:42795745-42795764 None:intergenic 45.0%
! GCACTGATGCGAGGATATTT+GGG + chr4.4:42795779-42795798 None:intergenic 45.0%
! GTCAAGCTTGTTTCTTGGTG+TGG + chr4.4:42795801-42795820 None:intergenic 45.0%
!! CTTCGCATGTTTTGTTAGGG+TGG - chr4.4:42796087-42796106 MS.gene61130:intron 45.0%
!! TTTCTTCATAGCGGACTCAG+TGG + chr4.4:42795852-42795871 None:intergenic 45.0%
AGGTGTTAACCTTCTTCGCC+TGG + chr4.4:42795964-42795983 None:intergenic 50.0%
CAATGCCTGTGCCTTTGGAT+CGG + chr4.4:42795648-42795667 None:intergenic 50.0%
CAGTGCAATGCTATCAGGGT+TGG - chr4.4:42796333-42796352 MS.gene61130:intron 50.0%
GCTTTCAATGCCTGTGCCTT+TGG + chr4.4:42795653-42795672 None:intergenic 50.0%
GTATGACATCCAGGCGAAGA+AGG - chr4.4:42795952-42795971 MS.gene61130:intron 50.0%
TAGGCCTTCTTGGTACCATC+GGG + chr4.4:42796478-42796497 None:intergenic 50.0%
TTAATCTGTTGCAGGCCCGA+TGG - chr4.4:42796460-42796479 MS.gene61130:intron 50.0%
!! ACAGGCATTGAAAGCTGCCA+AGG - chr4.4:42795658-42795677 MS.gene61130:intron 50.0%
!! GGCACTGATGCGAGGATATT+TGG + chr4.4:42795780-42795799 None:intergenic 50.0%
! AGTGCCTGACTTGACTGCCT+TGG + chr4.4:42795678-42795697 None:intergenic 55.0%
! GAAGACCGATCCAAAGGCAC+AGG - chr4.4:42795640-42795659 MS.gene61130:intron 55.0%
!! ATAGCGGACTCAGTGGTGAG+TGG + chr4.4:42795845-42795864 None:intergenic 55.0%
!! TAGCGGACTCAGTGGTGAGT+GGG + chr4.4:42795844-42795863 None:intergenic 55.0%
CAGGCCCGATGGTACCAAGA+AGG - chr4.4:42796471-42796490 MS.gene61130:intron 60.0%
GCTGCCAAGGCAGTCAAGTC+AGG - chr4.4:42795671-42795690 MS.gene61130:intron 60.0%
!! CTTGGTGTGGCACTGATGCG+AGG + chr4.4:42795788-42795807 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 42794553 42796558 42794553 ID=MS.gene61130
chr4.4 mRNA 42794553 42796558 42794553 ID=MS.gene61130.t1;Parent=MS.gene61130
chr4.4 exon 42796546 42796558 42796546 ID=MS.gene61130.t1.exon1;Parent=MS.gene61130.t1
chr4.4 CDS 42796546 42796558 42796546 ID=cds.MS.gene61130.t1;Parent=MS.gene61130.t1
chr4.4 exon 42795121 42795484 42795121 ID=MS.gene61130.t1.exon2;Parent=MS.gene61130.t1
chr4.4 CDS 42795121 42795484 42795121 ID=cds.MS.gene61130.t1;Parent=MS.gene61130.t1
chr4.4 exon 42794553 42794637 42794553 ID=MS.gene61130.t1.exon3;Parent=MS.gene61130.t1
chr4.4 CDS 42794553 42794637 42794553 ID=cds.MS.gene61130.t1;Parent=MS.gene61130.t1
Gene Sequence

>MS.gene61130

ATGGCTCCAAAAGGTAACTTCAATTTTCTGATCTCATTATTTTCCACTGATTTAAGTTTATGTGCTATTTCAATTTTCAACCATTCATTTTTGTTAGTGAAAAATGTTTTTGTATAATTTAGATTGAACTTAATCAATATGTAATTAATCGAAAAATGTTCATATGTTAATTGTATTATTGTGTAAGTCATTCCATTTTCATGTATGATTTGATTTTAGGAGTTTGTAAGCGACTGTTTAGGACTGTCCTGAGTTATGATGAGCAAAAAAAAGTTAGGTTTTACTCAGATAAATAGCTTAATTGAGTGCTTATGCTAGAATCGCTTATTAGCTATTTTTATAACAAAAGATACAAAAAAATTGTTTTCATTAAAGCTATAAGTTGTTTTCATAAGCTATCCTGAAGAACTTATAAAATTAAGGTGAAATTAGTGTATGGACATGTCAAAAGATGTTTCCATAAGCTCAAATTTGTAAATCCAAAACTATCCTTACATCCTGTTTGGTTAAGCAACTTATTTGCAGCTTATAGTACACACTTATTCATAAGCTTACATAAGCTATTTTTATAGGAAAAGATAAATTATAGTTAAATAGTTTTTCTATATGCTATGAGCTGTTTTTATAAGCTATATCGAAAAACTTGTGAAAACAAGCTAAAAAAAAGTTTGTGAAAATTGTCATTTCTGCTATAAGCTGTTTTTATAAGCTATATTCGAGAACGTATGAAAACAAGCTAAAAAAAATCTGTGAAAATTGTCATCAGCTGTTTTCATAAGATCTCCCAAACAGTCTCACACAACGTATGCTAGTAGATAATCTCAAATAAGCCAATCCAAACAGGCCCTTAATGTTATATCATAAGCTGTTTTTAAATAAGCTTTCTATTTAACTTACAGAATTATTGCTTAATCTCTCATATGATTAATACTGATTATCCATAGGCTTTTGGTTCAGTATATTTTAATCAAATAATGCATGGAATTAGGAATATTATTATATTACACAAAAAAATAGTTAGAACTTTGTTTTTTTTTTTTTTCTTCTTGTGACTTATGCAATGTTTTTGCACAGGTGATGTTTCTAAGAAGACCGATCCAAAGGCACAGGCATTGAAAGCTGCCAAGGCAGTCAAGTCAGGCACTGCTATTAAGAAGAAGGCAAAGAAGATTAGAACAACTGTAACTTTCCATCGCCCAAAGACACAAACAAGGGATAGGAACCCCAAATATCCTCGCATCAGTGCCACACCAAGAAACAAGCTTGACCACTATCAGATTTTGAAGTTCCCACTCACCACTGAGTCCGCTATGAAGAAAATTGAGGATAACAACACTTTGGTTTTCATTGTTGACTTGCGTGCTGATAAGAAAAAGATTAAGGATGCAGTGAAGAAAATGTATGACATCCAGGCGAAGAAGGTTAACACCTTGATCAGGTGAGCATTTATGCAGTTACTGTTCAAGGCAAATTAATAATCTGATAAATGCTAATAACAGTTGTAGTCTAATTGTCAAATTCATACTGCACTACCCTTCGCATGTTTTGTTAGGGTGGTACCTGACATTTGAGATAATTTGTAGTCCAGTAGTATATTTTGCAGACATACCGAACCATTTTTGGGTTTTGGCGGTTTTTTGTTTTCTAGTTGAATACGTGTTTAGGCATTAATACCAACAATGGTTTTCTGTTTTCTAGTTGAATATGTGTTTAGGCATTAATACCAACAATGGTTCTGTTTTCTAGTTGAATATGTGTTTAGGCATTAATACCAACAGTTCAGTGCAATGCTATCAGGGTTGGAATCTGGAATTAATGATCAGGGAGATTGCATAGTTATGCTATCTGTACAATTTAATTATTATAGTTCAGCATGGTGATTTCAGACAATTTTTAAACTCGACTTTTTAATCTGTTGCAGGCCCGATGGTACCAAGAAGGCCTATGTTAGGTTGACACCTGATTACGATGCATTGGATGTAGCAAACAAGATTGGGATCATCTAA
Protein sequence

>MS.gene61130.t1

MAPKGDVSKKTDPKAQALKAAKAVKSGTAIKKKAKKIRTTVTFHRPKTQTRDRNPKYPRISATPRNKLDHYQILKFPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII