AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031378

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031378.t1 MTR_1g012990 98.119 319 4 1 1 319 1 317 0.0 646
MS.gene031378.t1 MTR_7g069430 97.179 319 5 1 1 319 1 315 0.0 638
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031378.t1 AT3G15460 79.062 320 60 3 1 319 1 314 0.0 523
MS.gene031378.t1 AT1G52930 78.125 320 67 2 1 319 1 318 0.0 504

Find 69 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTAGGCGTATTGTTTAT+AGG 0.224171 2.1:-20029057 MS.gene031378:intron
CAATTGTTGATGACCATATA+TGG 0.275985 2.1:-20027008 MS.gene031378:CDS
AAAATATTCGGTGGCAGTTT+TGG 0.276214 2.1:-20025886 MS.gene031378:CDS
CGCTTTGCAGAAGAAGAAAA+AGG 0.301141 2.1:-20025706 MS.gene031378:CDS
ATGGAGGAATTGAAGCTTAC+TGG 0.304877 2.1:-20027668 MS.gene031378:CDS
TGGTTTGGCGACACATATAA+TGG 0.307289 2.1:+20025841 None:intergenic
GAGACCAGTGAGGACCCTTT+TGG 0.316595 2.1:-20029176 MS.gene031378:CDS
GGTAATTTCGGAACCATATA+TGG 0.326418 2.1:+20026995 None:intergenic
TGCATACTGGAAACTGTTAA+AGG 0.335855 2.1:-20027588 MS.gene031378:CDS
AGACACAAACCTGGTAATTT+CGG 0.336053 2.1:+20026983 None:intergenic
TTGAATCCAATTAAAATATT+CGG 0.338246 2.1:-20025898 MS.gene031378:CDS
TGAGAATGAAGTCGAGGTTA+AGG 0.351881 2.1:-20029131 MS.gene031378:CDS
GTTAAAGGAGATGCTGTTAC+AGG 0.353959 2.1:-20027573 MS.gene031378:intron
TTCTCTTTGTTCCTGAATAC+AGG 0.355373 2.1:+20029094 None:intergenic
ATGGTGCTTCACCTGTATTC+AGG 0.357859 2.1:-20029105 MS.gene031378:CDS
GCTTCTGCCTCATTGCAAAA+AGG 0.366733 2.1:-20028520 MS.gene031378:CDS
CTTACTGGGAATCACCTAAA+AGG 0.369692 2.1:-20027653 MS.gene031378:CDS
AGGGTCCTCACTGGTCTCTC+TGG 0.383639 2.1:+20029181 None:intergenic
GGAAAGGCTTAGCCTTTCTA+TGG 0.386528 2.1:+20027052 None:intergenic
ATAATGGTCATTACCTGGTT+TGG 0.395586 2.1:+20025826 None:intergenic
CAGGAACAAAGAGAAAGTTT+TGG 0.404050 2.1:-20029086 MS.gene031378:CDS
AATTTAACAGACGGACCGTT+GGG 0.405139 2.1:+20027896 None:intergenic
AGCTGTGGCTGTGCCCAAAA+AGG 0.410300 2.1:-20029215 MS.gene031378:CDS
CACATATCTGCAAACTCATC+AGG 0.414305 2.1:+20025612 None:intergenic
TTAGCCTTTCTATGGTCCTT+CGG 0.432964 2.1:+20027060 None:intergenic
GAGCTCAACAAGCTCGTTCA+AGG 0.438472 2.1:+20028461 None:intergenic
GGCTGGAACATTTGCAAAGA+AGG 0.442018 2.1:-20025685 MS.gene031378:CDS
AATGGATTCTCATATAAAGT+TGG 0.442109 2.1:+20025859 None:intergenic
TCTTATCCTTCCATCCCAAA+AGG 0.448553 2.1:+20029161 None:intergenic
GCATGAGATGGCCAATCCTC+TGG 0.463080 2.1:-20025637 MS.gene031378:CDS
ATGGATTCTCATATAAAGTT+GGG 0.466075 2.1:+20025860 None:intergenic
AGGCAAAAGATCTTTATCTC+TGG 0.470351 2.1:-20027931 MS.gene031378:CDS
TGATAAAATGACATTGATTG+AGG 0.479641 2.1:-20026022 MS.gene031378:intron
CTCATCAGGCTCCAGAGGAT+TGG 0.500537 2.1:+20025626 None:intergenic
CCGACATTTGATGTTGAACC+TGG 0.503744 2.1:-20028547 MS.gene031378:CDS
TTTGATGAAAATGTCAAAAG+AGG 0.513130 2.1:+20027623 None:intergenic
TATATGGTTCCGAAATTACC+AGG 0.519157 2.1:-20026992 MS.gene031378:intron
TGGAGGAATTGAAGCTTACT+GGG 0.524362 2.1:-20027667 MS.gene031378:CDS
CTTATCCTTCCATCCCAAAA+GGG 0.530204 2.1:+20029162 None:intergenic
ATTATATGTGTCGCCAAACC+AGG 0.539453 2.1:-20025839 MS.gene031378:intron
AAATTTAACAGACGGACCGT+TGG 0.547683 2.1:+20027895 None:intergenic
AATGAGGCAGAAGCGATACC+AGG 0.551260 2.1:+20028529 None:intergenic
CATCCCAAAAGGGTCCTCAC+TGG 0.551306 2.1:+20029172 None:intergenic
GAAGTCGAGGTTAAGGAGAA+TGG 0.558717 2.1:-20029124 MS.gene031378:CDS
GAAGAGAAAACGAAGCGAAA+AGG 0.560289 2.1:-20029242 MS.gene031378:CDS
AAAAGATCTTTATCTCTGGA+TGG 0.569362 2.1:-20027927 MS.gene031378:CDS
TTGATGAAAATGTCAAAAGA+GGG 0.581244 2.1:+20027624 None:intergenic
AATCCAATTAAAATATTCGG+TGG 0.581638 2.1:-20025895 MS.gene031378:CDS
GCAAACTCATCAGGCTCCAG+AGG 0.583650 2.1:+20025621 None:intergenic
AAGGAGGAAAATGCATGAGA+TGG 0.589573 2.1:-20025649 MS.gene031378:CDS
GTTGAATCGAAAGAAACTAA+AGG 0.591875 2.1:-20028489 MS.gene031378:CDS
AACTCCGAAGGACCATAGAA+AGG 0.592135 2.1:-20027064 MS.gene031378:CDS
TGAAAATGTCAAAAGAGGGC+GGG 0.598198 2.1:+20027628 None:intergenic
TACTTGTTTAGGTTGGTCCA+CGG 0.602021 2.1:-20025927 MS.gene031378:intron
CTGATGAGTTTGCAGATATG+TGG 0.604261 2.1:-20025611 MS.gene031378:CDS
AAGCGAAAAGGCTGAAGCTG+TGG 0.609947 2.1:-20029230 MS.gene031378:CDS
AGAAGGTCAAAGCAAAGACA+AGG 0.616949 2.1:-20025668 MS.gene031378:CDS
AATTTGTCTGACTCATTGTG+AGG 0.622655 2.1:+20026060 None:intergenic
TGAAAATGAGAATGAAGTCG+AGG 0.626692 2.1:-20029137 MS.gene031378:CDS
ATTGGATTCAAGCAGAACCG+TGG 0.628585 2.1:+20025910 None:intergenic
ATGAAAATGTCAAAAGAGGG+CGG 0.629935 2.1:+20027627 None:intergenic
AGGTCAAAGCAAAGACAAGG+AGG 0.640567 2.1:-20025665 MS.gene031378:CDS
TGGATGGCAAAATGCCCCAA+CGG 0.651109 2.1:-20027911 MS.gene031378:CDS
ATGTGAATTTCAGTGCAGGA+AGG 0.660599 2.1:-20027951 MS.gene031378:intron
CCAGGTTCAACATCAAATGT+CGG 0.661077 2.1:+20028547 None:intergenic
ATTTAACAGACGGACCGTTG+GGG 0.668767 2.1:+20027897 None:intergenic
CTCAACAAGCTCGTTCAAGG+TGG 0.686995 2.1:+20028464 None:intergenic
TTGCTCCAGAGAGACCAGTG+AGG 0.749614 2.1:-20029186 MS.gene031378:CDS
GAGTCAGACAAATTACCCCG+AGG 0.769107 2.1:-20026051 MS.gene031378:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAATTAATGTCAAA+TGG - chr2.1:20025980-20025999 MS.gene031378:intron 10.0%
!! AAATACAGCAAAATATATTT+GGG + chr2.1:20025832-20025851 None:intergenic 15.0%
!! AATAATATATAATCATAGCA+AGG + chr2.1:20027042-20027061 None:intergenic 15.0%
!! ATTGATCATAAACTTATTAT+AGG - chr2.1:20026277-20026296 MS.gene031378:intron 15.0%
!! TTGAATCCAATTAAAATATT+CGG - chr2.1:20028951-20028970 MS.gene031378:intron 15.0%
!!! CTACACTTTAAAATTTAAAA+TGG + chr2.1:20026687-20026706 None:intergenic 15.0%
!!! GATTATTTTTTATGACATTA+AGG - chr2.1:20026452-20026471 MS.gene031378:intron 15.0%
!!! TAATGCTTTAAAAATTTGAA+AGG + chr2.1:20028148-20028167 None:intergenic 15.0%
!!! TTATTTTCAGAAGAAAATTA+CGG + chr2.1:20029072-20029091 None:intergenic 15.0%
!!! TTGGTTATTTTTTAATGTAT+AGG - chr2.1:20028548-20028567 MS.gene031378:CDS 15.0%
!! AAAAGAGAAATTAGTAAGAT+GGG + chr2.1:20027135-20027154 None:intergenic 20.0%
!! AGTTCTATAAATGTTATGTT+TGG - chr2.1:20027531-20027550 MS.gene031378:intron 20.0%
!! CAAATACAGCAAAATATATT+TGG + chr2.1:20025833-20025852 None:intergenic 20.0%
!! CAGTTTACTTAATTTAAATC+TGG - chr2.1:20026761-20026780 MS.gene031378:intron 20.0%
!! CTAACTAAAAATTTAACAGA+CGG + chr2.1:20026965-20026984 None:intergenic 20.0%
!! GCTATGATTATATATTATTC+TGG - chr2.1:20027043-20027062 MS.gene031378:CDS 20.0%
!! TAAAAGAGAAATTAGTAAGA+TGG + chr2.1:20027136-20027155 None:intergenic 20.0%
!! TGTCATAAAAAATAATCGAA+AGG + chr2.1:20026449-20026468 None:intergenic 20.0%
!! TTTACTTAATTTAAATCTGG+CGG - chr2.1:20026764-20026783 MS.gene031378:intron 20.0%
!!! AAAATATATTTGGGTTTTGT+GGG + chr2.1:20025823-20025842 None:intergenic 20.0%
!!! AAGTAATGATTGTTTTACTA+TGG - chr2.1:20028318-20028337 MS.gene031378:intron 20.0%
!!! AATTTTTAAAGCATTATCCT+TGG - chr2.1:20028152-20028171 MS.gene031378:intron 20.0%
!!! ACATCAGTTAAATGAATTTT+AGG + chr2.1:20028894-20028913 None:intergenic 20.0%
!!! ATTGATAGTATATAAGGTTT+TGG + chr2.1:20028294-20028313 None:intergenic 20.0%
!!! ATTTTTAAAGCATTATCCTT+GGG - chr2.1:20028153-20028172 MS.gene031378:intron 20.0%
!!! TTCTGAAAATAACTTCATAT+TGG - chr2.1:20029080-20029099 MS.gene031378:CDS 20.0%
!!! TTCTTCATGTTTATTTTTTG+AGG - chr2.1:20026419-20026438 MS.gene031378:intron 20.0%
!!! TTCTTCATTGATAGTATATA+AGG + chr2.1:20028300-20028319 None:intergenic 20.0%
! AACTCAAACTTGAGATTAAA+TGG + chr2.1:20026805-20026824 None:intergenic 25.0%
! AATACATAGAAAAGCCAATT+TGG + chr2.1:20026737-20026756 None:intergenic 25.0%
! AATCCAATTAAAATATTCGG+TGG - chr2.1:20028954-20028973 MS.gene031378:intron 25.0%
! AATTTAAATTGAGCAGAATG+TGG - chr2.1:20027571-20027590 MS.gene031378:intron 25.0%
! ATAACCAAAATTAAGTTCAC+TGG + chr2.1:20028536-20028555 None:intergenic 25.0%
! ATACATAGAAAAGCCAATTT+GGG + chr2.1:20026736-20026755 None:intergenic 25.0%
! ATACTAAAGCCTATTTGTAA+AGG + chr2.1:20028181-20028200 None:intergenic 25.0%
! ATGGATTCTCATATAAAGTT+GGG + chr2.1:20028992-20029011 None:intergenic 25.0%
! ATTGATTATTTGTAAACCAC+TGG - chr2.1:20028363-20028382 MS.gene031378:intron 25.0%
! ATTTAAATTGAGCAGAATGT+GGG - chr2.1:20027572-20027591 MS.gene031378:intron 25.0%
! CAAAAAGAAGAATAAAGAAC+TGG + chr2.1:20027079-20027098 None:intergenic 25.0%
! CAACAACATTTGTAACAAAA+TGG + chr2.1:20026573-20026592 None:intergenic 25.0%
! CTGAGTTATGTATTTGAATA+GGG - chr2.1:20027644-20027663 MS.gene031378:CDS 25.0%
! GAAAATTCACTGTTTGTTTA+TGG - chr2.1:20026533-20026552 MS.gene031378:intron 25.0%
! TAATAGCTTAACTAAGTTGT+TGG + chr2.1:20025908-20025927 None:intergenic 25.0%
! TATAATCATAGCAAGGAAAT+TGG + chr2.1:20027035-20027054 None:intergenic 25.0%
! TGAGTTATGTATTTGAATAG+GGG - chr2.1:20027645-20027664 MS.gene031378:CDS 25.0%
! TTCACTCAAGTAAAAAACTA+TGG + chr2.1:20028056-20028075 None:intergenic 25.0%
! TTGATGAAAATGTCAAAAGA+GGG + chr2.1:20027228-20027247 None:intergenic 25.0%
! TTTGATGAAAATGTCAAAAG+AGG + chr2.1:20027229-20027248 None:intergenic 25.0%
!! AAAACTTCTTCCTTTTATTG+CGG - chr2.1:20026232-20026251 MS.gene031378:intron 25.0%
!! AATGGATTCTCATATAAAGT+TGG + chr2.1:20028993-20029012 None:intergenic 25.0%
!! AATTCTTTTACTGCACATAA+AGG + chr2.1:20026173-20026192 None:intergenic 25.0%
!! ATTCTTTTACTGCACATAAA+GGG + chr2.1:20026172-20026191 None:intergenic 25.0%
!! CATTTTGTTACAAATGTTGT+TGG - chr2.1:20026571-20026590 MS.gene031378:intron 25.0%
!! TAATCCAGTGAACTTAATTT+TGG - chr2.1:20028529-20028548 MS.gene031378:CDS 25.0%
!! TATAATTATGCCAGTTGTTT+TGG - chr2.1:20028026-20028045 MS.gene031378:intron 25.0%
!! TCGTGATATCATATTTCTAT+TGG + chr2.1:20028244-20028263 None:intergenic 25.0%
!! TGATAAAATGACATTGATTG+AGG - chr2.1:20028827-20028846 MS.gene031378:intron 25.0%
!! TTGATAATACGATCTTAGTT+TGG - chr2.1:20027355-20027374 MS.gene031378:intron 25.0%
!!! ATTTTGAAAAAGATGCATAC+TGG - chr2.1:20027248-20027267 MS.gene031378:intron 25.0%
!!! CAAAATATATTTGGGTTTTG+TGG + chr2.1:20025824-20025843 None:intergenic 25.0%
!!! CAATCAATGTCATTTTATCA+AGG + chr2.1:20028827-20028846 None:intergenic 25.0%
!!! GTTAAATTTTTAGTTAGTGC+CGG - chr2.1:20026968-20026987 MS.gene031378:intron 25.0%
!!! GTTTAAACTTTAAAATCCGT+TGG - chr2.1:20027950-20027969 MS.gene031378:intron 25.0%
!!! TGTTTTGGATTCAAAAGTTA+AGG - chr2.1:20027327-20027346 MS.gene031378:intron 25.0%
!!! TGTTTTTAACCTCTTTATGT+TGG - chr2.1:20029106-20029125 MS.gene031378:CDS 25.0%
!!! TTCACAATTTTCTCTAGTTT+TGG - chr2.1:20027612-20027631 MS.gene031378:CDS 25.0%
!!! TTGTTTATTTTGGCTATGTT+TGG - chr2.1:20025882-20025901 MS.gene031378:CDS 25.0%
AAAAGATCTTTATCTCTGGA+TGG - chr2.1:20026922-20026941 MS.gene031378:intron 30.0%
AAACACTTGTAAGACTGTTT+GGG + chr2.1:20026110-20026129 None:intergenic 30.0%
AACTAGAGAAAATTGTGAAC+AGG + chr2.1:20027611-20027630 None:intergenic 30.0%
AAGAAGCTGAAAACAGTTTA+TGG - chr2.1:20026055-20026074 MS.gene031378:CDS 30.0%
AATCTCTTTCCTTTATGTGA+TGG - chr2.1:20026612-20026631 MS.gene031378:intron 30.0%
ACTTGCTTAAGATTAAGAAC+AGG + chr2.1:20026828-20026847 None:intergenic 30.0%
AGTTAAGCTATTATGCTGTA+AGG - chr2.1:20025915-20025934 MS.gene031378:CDS 30.0%
ATCCATTATCCATCACATAA+AGG + chr2.1:20026624-20026643 None:intergenic 30.0%
CAAACAGATGAGAATATTAC+CGG + chr2.1:20026990-20027009 None:intergenic 30.0%
CAATTGAGAAAACAAAAACG+TGG + chr2.1:20027827-20027846 None:intergenic 30.0%
CCTATTCAAATACATAACTC+AGG + chr2.1:20027646-20027665 None:intergenic 30.0%
CCTGAGTTATGTATTTGAAT+AGG - chr2.1:20027643-20027662 MS.gene031378:CDS 30.0%
GTTAAGCTATTATGCTGTAA+GGG - chr2.1:20025916-20025935 MS.gene031378:CDS 30.0%
GTTGAATCGAAAGAAACTAA+AGG - chr2.1:20026360-20026379 MS.gene031378:intron 30.0%
TAAACACTTGTAAGACTGTT+TGG + chr2.1:20026111-20026130 None:intergenic 30.0%
TACATAATAATAGCATCCAG+TGG + chr2.1:20028382-20028401 None:intergenic 30.0%
TATAGAACTTCTCAAGTCAA+CGG + chr2.1:20027520-20027539 None:intergenic 30.0%
TCATTGCAAAAAGGATAACA+AGG - chr2.1:20026338-20026357 MS.gene031378:intron 30.0%
TCTAGTCTATTTGTGACATT+TGG - chr2.1:20026847-20026866 MS.gene031378:intron 30.0%
TGTTGAAAACAACAAACCAA+CGG + chr2.1:20027969-20027988 None:intergenic 30.0%
! AAACTTCTTCCTTTTATTGC+GGG - chr2.1:20026233-20026252 MS.gene031378:intron 30.0%
! ATAATACGATCTTAGTTTGG+TGG - chr2.1:20027358-20027377 MS.gene031378:intron 30.0%
! TAAACAATGCTATTTGATGC+TGG - chr2.1:20028582-20028601 MS.gene031378:intron 30.0%
! TTGTTATCCTTTTTGCAATG+AGG + chr2.1:20026339-20026358 None:intergenic 30.0%
!! ATTGTTTTACTATGGTTGCA+TGG - chr2.1:20028326-20028345 MS.gene031378:intron 30.0%
!! CAATTGTTGATGACCATATA+TGG - chr2.1:20027841-20027860 MS.gene031378:intron 30.0%
!! CTCATCTGTTTGTTATCTTA+AGG - chr2.1:20026998-20027017 MS.gene031378:CDS 30.0%
!! GAGTTCGGATTTGTTTATTT+TGG - chr2.1:20025872-20025891 MS.gene031378:CDS 30.0%
!! TTCCTTTATGTGATGGATAA+TGG - chr2.1:20026619-20026638 MS.gene031378:intron 30.0%
!!! GGGTTATTGGTTTTTGATTA+TGG - chr2.1:20026253-20026272 MS.gene031378:intron 30.0%
!!! GTTTGTGTTTGTTTTGAGTT+CGG - chr2.1:20025857-20025876 MS.gene031378:CDS 30.0%
AAAAATAAGCGCAAGCATCT+TGG - chr2.1:20028002-20028021 MS.gene031378:intron 35.0%
AAACTACCTTGAAGAGCTTA+TGG - chr2.1:20026032-20026051 MS.gene031378:CDS 35.0%
AACATTGCCGATGGATAAAT+TGG - chr2.1:20028113-20028132 MS.gene031378:intron 35.0%
AACCAATAACCCGCAATAAA+AGG + chr2.1:20026245-20026264 None:intergenic 35.0%
AAGTGTGCACAGAGAAATAA+TGG + chr2.1:20029042-20029061 None:intergenic 35.0%
AATTTGTCTGACTCATTGTG+AGG + chr2.1:20028792-20028811 None:intergenic 35.0%
ACATTGCCGATGGATAAATT+GGG - chr2.1:20028114-20028133 MS.gene031378:intron 35.0%
AGAAAACAAAAACGTGGTCA+TGG + chr2.1:20027821-20027840 None:intergenic 35.0%
AGACACAAACCTGGTAATTT+CGG + chr2.1:20027869-20027888 None:intergenic 35.0%
AGGCAAAAGATCTTTATCTC+TGG - chr2.1:20026918-20026937 MS.gene031378:intron 35.0%
ATAATGGTCATTACCTGGTT+TGG + chr2.1:20029026-20029045 None:intergenic 35.0%
ATAGAAAAGCCAATTTGGGA+GGG + chr2.1:20026732-20026751 None:intergenic 35.0%
ATGAAAATGTCAAAAGAGGG+CGG + chr2.1:20027225-20027244 None:intergenic 35.0%
ATTGATTGAGGTCTGCAAAT+TGG - chr2.1:20028839-20028858 MS.gene031378:intron 35.0%
CACAAACCCAATTTATCCAT+CGG + chr2.1:20028123-20028142 None:intergenic 35.0%
CTACAACAACCAACATAAAG+AGG + chr2.1:20029118-20029137 None:intergenic 35.0%
CTGAAATTCACATACACAGA+AGG + chr2.1:20026891-20026910 None:intergenic 35.0%
GAGAAATAATGGTCATTACC+TGG + chr2.1:20029031-20029050 None:intergenic 35.0%
GGTAATTTCGGAACCATATA+TGG + chr2.1:20027857-20027876 None:intergenic 35.0%
TATATGGTTCCGAAATTACC+AGG - chr2.1:20027857-20027876 MS.gene031378:intron 35.0%
TCTGCATGAGAATAAAAACG+TGG + chr2.1:20028706-20028725 None:intergenic 35.0%
TGAAAATGAGAATGAAGTCG+AGG - chr2.1:20025712-20025731 MS.gene031378:CDS 35.0%
TGCAAACTACTTGTTTAGGT+TGG - chr2.1:20028915-20028934 MS.gene031378:intron 35.0%
TGCATACTGGAAACTGTTAA+AGG - chr2.1:20027261-20027280 MS.gene031378:intron 35.0%
TGTGTGCAAACTACTTGTTT+AGG - chr2.1:20028911-20028930 MS.gene031378:intron 35.0%
TTCTCTTTGTTCCTGAATAC+AGG + chr2.1:20025758-20025777 None:intergenic 35.0%
! AGAAATTAGTAAGATGGGCT+CGG + chr2.1:20027130-20027149 None:intergenic 35.0%
! CAGGAACAAAGAGAAAGTTT+TGG - chr2.1:20025763-20025782 MS.gene031378:intron 35.0%
! GTATGATTGATGGATCTACA+GGG - chr2.1:20028490-20028509 MS.gene031378:CDS 35.0%
! TACTCTTCTATGCACTGTTT+TGG - chr2.1:20027312-20027331 MS.gene031378:intron 35.0%
! TCATTTTATCAAGGCTTCCT+CGG + chr2.1:20028818-20028837 None:intergenic 35.0%
!! AAAATATTCGGTGGCAGTTT+TGG - chr2.1:20028963-20028982 MS.gene031378:intron 35.0%
!! TTCCTTTTATTGCGGGTTAT+TGG - chr2.1:20026240-20026259 MS.gene031378:intron 35.0%
!!! AAGTTTTGGTTACTTGCTCT+AGG - chr2.1:20025777-20025796 MS.gene031378:intron 35.0%
!!! CTGCCACCGAATATTTTAAT+TGG + chr2.1:20028960-20028979 None:intergenic 35.0%
AAATTTAACAGACGGACCGT+TGG + chr2.1:20026957-20026976 None:intergenic 40.0%
AAGGAGGAAAATGCATGAGA+TGG - chr2.1:20029200-20029219 MS.gene031378:CDS 40.0%
AATTTAACAGACGGACCGTT+GGG + chr2.1:20026956-20026975 None:intergenic 40.0%
ACAAAAACGTGGTCATGGAA+AGG + chr2.1:20027816-20027835 None:intergenic 40.0%
AGAAGGTCAAAGCAAAGACA+AGG - chr2.1:20029181-20029200 MS.gene031378:CDS 40.0%
ATGGAGGAATTGAAGCTTAC+TGG - chr2.1:20027181-20027200 MS.gene031378:intron 40.0%
ATGTGAATTTCAGTGCAGGA+AGG - chr2.1:20026898-20026917 MS.gene031378:intron 40.0%
ATTATATGTGTCGCCAAACC+AGG - chr2.1:20029010-20029029 MS.gene031378:intron 40.0%
CAATTTGGGAGGGAAAACAA+AGG + chr2.1:20026722-20026741 None:intergenic 40.0%
CACATATCTGCAAACTCATC+AGG + chr2.1:20029240-20029259 None:intergenic 40.0%
CATAGAAAAGCCAATTTGGG+AGG + chr2.1:20026733-20026752 None:intergenic 40.0%
CCAGGTTCAACATCAAATGT+CGG + chr2.1:20026305-20026324 None:intergenic 40.0%
CCTATTTGTAAAGGAACCCA+AGG + chr2.1:20028172-20028191 None:intergenic 40.0%
CCTTCGGAGTTTCAAAAATC+TGG + chr2.1:20027776-20027795 None:intergenic 40.0%
CCTTGGGTTCCTTTACAAAT+AGG - chr2.1:20028169-20028188 MS.gene031378:intron 40.0%
CGCTTTGCAGAAGAAGAAAA+AGG - chr2.1:20029143-20029162 MS.gene031378:CDS 40.0%
CTATGGCTAACCAAAACAAC+TGG + chr2.1:20028039-20028058 None:intergenic 40.0%
CTGATGAGTTTGCAGATATG+TGG - chr2.1:20029238-20029257 MS.gene031378:CDS 40.0%
CTTACTGGGAATCACCTAAA+AGG - chr2.1:20027196-20027215 MS.gene031378:intron 40.0%
CTTATCCTTCCATCCCAAAA+GGG + chr2.1:20025690-20025709 None:intergenic 40.0%
CTTCTTCCATAAGCTCTTCA+AGG + chr2.1:20026041-20026060 None:intergenic 40.0%
CTTGCATTTCTTTGCGAATG+TGG - chr2.1:20028609-20028628 MS.gene031378:intron 40.0%
GAAGAGAAAACGAAGCGAAA+AGG - chr2.1:20025607-20025626 MS.gene031378:CDS 40.0%
GAGATGCTGTTACAGGTATT+TGG - chr2.1:20027283-20027302 MS.gene031378:intron 40.0%
GTAGATCCATCAATCATACG+TGG + chr2.1:20028489-20028508 None:intergenic 40.0%
GTGTATGTGAATTTCAGTGC+AGG - chr2.1:20026894-20026913 MS.gene031378:intron 40.0%
GTTAAAGGAGATGCTGTTAC+AGG - chr2.1:20027276-20027295 MS.gene031378:intron 40.0%
GTTGTCTACACTTCTAGTCT+AGG - chr2.1:20028407-20028426 MS.gene031378:intron 40.0%
TCTTATCCTTCCATCCCAAA+AGG + chr2.1:20025691-20025710 None:intergenic 40.0%
TGAAAATGTCAAAAGAGGGC+GGG + chr2.1:20027224-20027243 None:intergenic 40.0%
TGAGAATGAAGTCGAGGTTA+AGG - chr2.1:20025718-20025737 MS.gene031378:intron 40.0%
TGCAAATTCAGACACAAACC+TGG + chr2.1:20027878-20027897 None:intergenic 40.0%
TGGAGGAATTGAAGCTTACT+GGG - chr2.1:20027182-20027201 MS.gene031378:intron 40.0%
TTCTTTGCTAACATTGCCGA+TGG - chr2.1:20028104-20028123 MS.gene031378:intron 40.0%
TTGCAGAAGAAGAAAAAGGC+TGG - chr2.1:20029147-20029166 MS.gene031378:CDS 40.0%
! ATTTTATCAAGGCTTCCTCG+GGG + chr2.1:20028816-20028835 None:intergenic 40.0%
! CATTTTATCAAGGCTTCCTC+GGG + chr2.1:20028817-20028836 None:intergenic 40.0%
! CGTATGATTGATGGATCTAC+AGG - chr2.1:20028489-20028508 MS.gene031378:CDS 40.0%
! GCTCTAGGCGTATTGTTTAT+AGG - chr2.1:20025792-20025811 MS.gene031378:intron 40.0%
! GGAGCAACATCTTCCTTTTT+GGG + chr2.1:20025650-20025669 None:intergenic 40.0%
! TGGAGCAACATCTTCCTTTT+TGG + chr2.1:20025651-20025670 None:intergenic 40.0%
! TGGTTTGGCGACACATATAA+TGG + chr2.1:20029011-20029030 None:intergenic 40.0%
! TTAGCCTTTCTATGGTCCTT+CGG + chr2.1:20027792-20027811 None:intergenic 40.0%
! TTTATGTGCGCACTTTTCTG+AGG - chr2.1:20027431-20027450 MS.gene031378:intron 40.0%
!! TACTTGTTTAGGTTGGTCCA+CGG - chr2.1:20028922-20028941 MS.gene031378:intron 40.0%
!!! CCAGATTTTTGAAACTCCGA+AGG - chr2.1:20027773-20027792 MS.gene031378:intron 40.0%
!!! CTTAGTTTGGTGGTGCTTTT+GGG - chr2.1:20027368-20027387 MS.gene031378:intron 40.0%
!!! CTTTGTTTTCCCTCCCAAAT+TGG - chr2.1:20026720-20026739 MS.gene031378:intron 40.0%
!!! GCGCACATGCATTTTTTTCT+TGG - chr2.1:20027686-20027705 MS.gene031378:intron 40.0%
!!! TCTTAGTTTGGTGGTGCTTT+TGG - chr2.1:20027367-20027386 MS.gene031378:intron 40.0%
!!! TTAGTTTGGTGGTGCTTTTG+GGG - chr2.1:20027369-20027388 MS.gene031378:intron 40.0%
AACTCCGAAGGACCATAGAA+AGG - chr2.1:20027785-20027804 MS.gene031378:intron 45.0%
ACGCTACCACGTATGATTGA+TGG - chr2.1:20028480-20028499 MS.gene031378:CDS 45.0%
AGGTCAAAGCAAAGACAAGG+AGG - chr2.1:20029184-20029203 MS.gene031378:CDS 45.0%
ATGGTGCTTCACCTGTATTC+AGG - chr2.1:20025744-20025763 MS.gene031378:intron 45.0%
ATTGGATTCAAGCAGAACCG+TGG + chr2.1:20028942-20028961 None:intergenic 45.0%
ATTTAACAGACGGACCGTTG+GGG + chr2.1:20026955-20026974 None:intergenic 45.0%
CCGACATTTGATGTTGAACC+TGG - chr2.1:20026302-20026321 MS.gene031378:intron 45.0%
GAAGTCGAGGTTAAGGAGAA+TGG - chr2.1:20025725-20025744 MS.gene031378:intron 45.0%
GCAAGACACAAGAGTCAGAT+GGG + chr2.1:20026649-20026668 None:intergenic 45.0%
GCTTCTGCCTCATTGCAAAA+AGG - chr2.1:20026329-20026348 MS.gene031378:intron 45.0%
GGCTGGAACATTTGCAAAGA+AGG - chr2.1:20029164-20029183 MS.gene031378:CDS 45.0%
TGCAAGACACAAGAGTCAGA+TGG + chr2.1:20026650-20026669 None:intergenic 45.0%
TGTGCACTGAAAAGCAGTGA+AGG + chr2.1:20027159-20027178 None:intergenic 45.0%
! AATCATACGTGGTAGCGTCA+TGG + chr2.1:20028478-20028497 None:intergenic 45.0%
! CTGCACATAAAGGGCTTGTT+TGG + chr2.1:20026163-20026182 None:intergenic 45.0%
! CTTTTCAGTGCACACAATGG+AGG - chr2.1:20027165-20027184 MS.gene031378:intron 45.0%
! GGAAAGGCTTAGCCTTTCTA+TGG + chr2.1:20027800-20027819 None:intergenic 45.0%
!! ATATTCGGTGGCAGTTTTGG+AGG - chr2.1:20028966-20028985 MS.gene031378:intron 45.0%
!! CTGCTTTTCAGTGCACACAA+TGG - chr2.1:20027162-20027181 MS.gene031378:intron 45.0%
AAGCGAAAAGGCTGAAGCTG+TGG - chr2.1:20025619-20025638 MS.gene031378:CDS 50.0%
AATGAGGCAGAAGCGATACC+AGG + chr2.1:20026323-20026342 None:intergenic 50.0%
CTCAACAAGCTCGTTCAAGG+TGG + chr2.1:20026388-20026407 None:intergenic 50.0%
GAGCTCAACAAGCTCGTTCA+AGG + chr2.1:20026391-20026410 None:intergenic 50.0%
GAGTCAGACAAATTACCCCG+AGG - chr2.1:20028798-20028817 MS.gene031378:intron 50.0%
TGGATGGCAAAATGCCCCAA+CGG - chr2.1:20026938-20026957 MS.gene031378:intron 50.0%
! AGACCAGTGAGGACCCTTTT+GGG - chr2.1:20025674-20025693 MS.gene031378:CDS 50.0%
AGCTGTGGCTGTGCCCAAAA+AGG - chr2.1:20025634-20025653 MS.gene031378:CDS 55.0%
CATCCCAAAAGGGTCCTCAC+TGG + chr2.1:20025680-20025699 None:intergenic 55.0%
CTCATCAGGCTCCAGAGGAT+TGG + chr2.1:20029226-20029245 None:intergenic 55.0%
GCAAACTCATCAGGCTCCAG+AGG + chr2.1:20029231-20029250 None:intergenic 55.0%
GCATGAGATGGCCAATCCTC+TGG - chr2.1:20029212-20029231 MS.gene031378:CDS 55.0%
TTGCTCCAGAGAGACCAGTG+AGG - chr2.1:20025663-20025682 MS.gene031378:CDS 55.0%
! AAAGAGGGCGGGAGCCTTTT+AGG + chr2.1:20027213-20027232 None:intergenic 55.0%
! GAGACCAGTGAGGACCCTTT+TGG - chr2.1:20025673-20025692 MS.gene031378:CDS 55.0%
!! CAGTGAGGACCCTTTTGGGA+TGG - chr2.1:20025678-20025697 MS.gene031378:CDS 55.0%
!!! GAGGACCCTTTTGGGATGGA+AGG - chr2.1:20025682-20025701 MS.gene031378:CDS 55.0%
! AGGGTCCTCACTGGTCTCTC+TGG + chr2.1:20025671-20025690 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 20025602 20029269 20025602 ID=MS.gene031378
chr2.1 mRNA 20025602 20029269 20025602 ID=MS.gene031378.t1;Parent=MS.gene031378
chr2.1 exon 20029058 20029269 20029058 ID=MS.gene031378.t1.exon1;Parent=MS.gene031378.t1
chr2.1 CDS 20029058 20029269 20029058 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20028431 20028572 20028431 ID=MS.gene031378.t1.exon2;Parent=MS.gene031378.t1
chr2.1 CDS 20028431 20028572 20028431 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20027882 20027960 20027882 ID=MS.gene031378.t1.exon3;Parent=MS.gene031378.t1
chr2.1 CDS 20027882 20027960 20027882 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20027574 20027698 20027574 ID=MS.gene031378.t1.exon4;Parent=MS.gene031378.t1
chr2.1 CDS 20027574 20027698 20027574 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20026993 20027094 20026993 ID=MS.gene031378.t1.exon5;Parent=MS.gene031378.t1
chr2.1 CDS 20026993 20027094 20026993 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20026023 20026091 20026023 ID=MS.gene031378.t1.exon6;Parent=MS.gene031378.t1
chr2.1 CDS 20026023 20026091 20026023 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20025840 20025938 20025840 ID=MS.gene031378.t1.exon7;Parent=MS.gene031378.t1
chr2.1 CDS 20025840 20025938 20025840 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
chr2.1 exon 20025602 20025733 20025602 ID=MS.gene031378.t1.exon8;Parent=MS.gene031378.t1
chr2.1 CDS 20025602 20025733 20025602 ID=cds.MS.gene031378.t1;Parent=MS.gene031378.t1
Gene Sequence

>MS.gene031378

ATGGGGAAGAGAAAACGAAGCGAAAAGGCTGAAGCTGTGGCTGTGCCCAAAAAGGAAGATGTTGCTCCAGAGAGACCAGTGAGGACCCTTTTGGGATGGAAGGATAAGAATGAAAATGAGAATGAAGTCGAGGTTAAGGAGAATGGTGCTTCACCTGTATTCAGGAACAAAGAGAAAGTTTTGGTTACTTGCTCTAGGCGTATTGTTTATAGGTACAACCCACAAAACCCAAATATATTTTGCTGTATTTGCACAGTTTGTGTTTGTTTTGAGTTCGGATTTGTTTATTTTGGCTATGTTTGGCCAACAACTTAGTTAAGCTATTATGCTGTAAGGGTTTATCATGTAAGTGCTTCTTTATAAATTATTTATGTAACAAAAATTAAATTAATGTCAAATGGTTTTGATATAAGCTATAAGTTGTATTCATAAACTACCTTGAAGAGCTTATGGAAGAAGCTGAAAACAGTTTATGGACATGTCATATGTTGTTTCTAGAAGCTGTCCCAAACAGTCTTACAAGTGTTTATTCTTGTAGATAAATTTAAATAATGCAATCCAAACAAGCCCTTTATGTGCAGTAAAAGAATTGTATCAGAATGCTCTTGTTTGTATGATATTAAGTAATAAAAAACTTCTTCCTTTTATTGCGGGTTATTGGTTTTTGATTATGGTATTGATCATAAACTTATTATAGGTACCGACATTTGATGTTGAACCTGGTATCGCTTCTGCCTCATTGCAAAAAGGATAACAAGGTTGAATCGAAAGAAACTAAAGGCGCCACCTTGAACGAGCTTGTTGAGCTCAAAAACTGTTCTTCATGTTTATTTTTTGAGGTATTCCTTTCGATTATTTTTTATGACATTAAGGTTTTTCATTGACAACTATGCATTATGTTTATGAGCTATTCTATGTTGTCGTTGAGTTAGAAAATTCACTGTTTGTTTATGGTGTGAAGAATTATTCCATTTTGTTACAAATGTTGTTGGTTAGTTTCAAATTGATATAATCTCTTTCCTTTATGTGATGGATAATGGATTACCCATCTGACTCTTGTGTCTTGCAGATTTTTGTTTTATCCATTTTAAATTTTAAAGTGTAGAACACTTTCACTCCTTTGTTTTCCCTCCCAAATTGGCTTTTCTATGTATTTACACAGTTTACTTAATTTAAATCTGGCGGCTGAGACAACATTTTCCATTTAATCTCAAGTTTGAGTTCCTGTTCTTAATCTTAAGCAAGTCTAGTCTATTTGTGACATTTGGATAACTATCTTATATCTACCTTCTGTGTATGTGAATTTCAGTGCAGGAAGGCAAAAGATCTTTATCTCTGGATGGCAAAATGCCCCAACGGTCCGTCTGTTAAATTTTTAGTTAGTGCCGGTAATATTCTCATCTGTTTGTTATCTTAAGGTTACTACATAACCAATTTCCTTGCTATGATTATATATTATTCTGGTATCAGTAATCCAGTTCTTTATTCTTCTTTTTGAATTATGATCGTCTCTCTTTCATTCTCTCCGAGCCCATCTTACTAATTTCTCTTTTACCTTCACTGCTTTTCAGTGCACACAATGGAGGAATTGAAGCTTACTGGGAATCACCTAAAAGGCTCCCGCCCTCTTTTGACATTTTCATCAAATTTTGAAAAAGATGCATACTGGAAACTGTTAAAGGAGATGCTGTTACAGGTATTTGGTTTGATTACTCTTCTATGCACTGTTTTGGATTCAAAAGTTAAGGTTATTTTGATAATACGATCTTAGTTTGGTGGTGCTTTTGGGGTTTTGCGTTGTATGATTTACTAGTATGATAGAAAGTTGTTTTATGTGCGCACTTTTCTGAGGTATTTGTGATCTTATAGTACAATTGTCATGTATCTATATGCTTAAAAGTATCTTAATCTGTCACCGTTGACTTGAGAAGTTCTATAAATGTTATGTTTGGCTCTTGTCAAAGTTTAAAATTTAAATTGAGCAGAATGTGGGACAAGCATTTATTCCTGTTCACAATTTTCTCTAGTTTTGGTTCTTGTACCTGAGTTATGTATTTGAATAGGGGTGTGAATTGTGTGAGCGTGCGCACATGCATTTTTTTCTTGGTTATTAGAATAATCAAAGTATACACAATTTTGATTATTGCTGATGTAGTCTTTCTTTTCTTTAACCAGATTTTTGAAACTCCGAAGGACCATAGAAAGGCTAAGCCTTTCCATGACCACGTTTTTGTTTTCTCAATTGTTGATGACCATATATGGTTCCGAAATTACCAGGTTTGTGTCTGAATTTGCAAAAGATCTTAATAATTGACATAACATTGTTTTCAAATTTGCATTATTTTAGAGTTTAAACTTTAAAATCCGTTGGTTTGTTGTTTTCAACATCACTCTACTAAAAAAAATAAGCGCAAGCATCTTGGTTATAATTATGCCAGTTGTTTTGGTTAGCCATAGTTTTTTACTTGAGTGAAATTGAGATGACAAATCTAAATGTTCTCTTTCTTTGCTAACATTGCCGATGGATAAATTGGGTTTGTGAGCCTTTCAAATTTTTAAAGCATTATCCTTGGGTTCCTTTACAAATAGGCTTTAGTATATGAAATAAAAATATACTTAAAACAAAATTACTTTGAAGACCAATAGAAATATGATATCACGATGAGTCTAGATTTGATGTAAAATCAAACCAAAACCTTATATACTATCAATGAAGAAGTAATGATTGTTTTACTATGGTTGCATGGAAAGCTTTCAACTGATTGATTATTTGTAAACCACTGGATGCTATTATTATGTAATTACGTTGTCTACACTTCTAGTCTAGGAGCAAAGAAAATGCATTCTTCACTTAGATATGCATGTCTTAAACACCATGACGCTACCACGTATGATTGATGGATCTACAGGGTTACTTACTTTTATACTAATCCAGTGAACTTAATTTTGGTTATTTTTTAATGTATAGGTCTTAATAATTTAAACAATGCTATTTGATGCTGGTACACTTGCATTTCTTTGCGAATGTGGCTTTGCAATGCTATTCTCATTTGCTGCTCTATGTTGTCATTGATGCTTACTGAACAATAAAACATAATAAACCACGTTTTTATTCTCATGCAGATTGATGCTTACTGAACAATAAAACATAATAAACCACGTTTTTATTCTCATGCAGATCTCTGTTCCTCACAATGAGTCAGACAAATTACCCCGAGGAAGCCTTGATAAAATGACATTGATTGAGGTCTGCAAATTGGCTCATTGATTTATCTTTGATCTGTCAATTCCTAAAATTCATTTAACTGATGTGTGCAAACTACTTGTTTAGGTTGGTCCACGGTTCTGCTTGAATCCAATTAAAATATTCGGTGGCAGTTTTGGAGGCCCAACTTTATATGAGAATCCATTATATGTGTCGCCAAACCAGGTAATGACCATTATTTCTCTGTGCACACTTATAATCTCCGTAATTTTCTTCTGAAAATAACTTCATATTGGTTGTGTTTTTAACCTCTTTATGTTGGTTGTTGTAGATTCGCGCTTTGCAGAAGAAGAAAAAGGCTGGAACATTTGCAAAGAAGGTCAAAGCAAAGACAAGGAGGAAAATGCATGAGATGGCCAATCCTCTGGAGCCTGATGAGTTTGCAGATATGTGGAAAGATTAA
Protein sequence

>MS.gene031378.t1

MGKRKRSEKAEAVAVPKKEDVAPERPVRTLLGWKDKNENENEVEVKENGASPVFRNKEKVLVTCSRRIVYRYRHLMLNLVSLLPHCKKDNKVESKETKGATLNELVELKNCSSCLFFECRKAKDLYLWMAKCPNGPSVKFLVSAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAYWKLLKEMLLQIFETPKDHRKAKPFHDHVFVFSIVDDHIWFRNYQISVPHNESDKLPRGSLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALQKKKKAGTFAKKVKAKTRRKMHEMANPLEPDEFADMWKD