AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060923

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060923.t1 MTR_1g012990 98.119 319 4 1 1 319 1 317 0.0 646
MS.gene060923.t1 MTR_7g069430 97.179 319 5 1 1 319 1 315 0.0 638
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060923.t1 AT3G15460 79.062 320 60 3 1 319 1 314 0.0 523
MS.gene060923.t1 AT1G52930 78.125 320 67 2 1 319 1 318 0.0 504

Find 71 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATCCAATTAAAATATT+TGG 0.145514 1.2:-8666556 MS.gene060923:CDS
AAAATATTTGGTGGCAGTTT+TGG 0.230031 1.2:-8666544 MS.gene060923:CDS
CAATTGTTGATGACCATATA+TGG 0.275985 1.2:-8667621 MS.gene060923:CDS
GCTCTAGGCGTATTGTTTAC+AGG 0.279178 1.2:-8669690 MS.gene060923:intron
ATGGAGGAATTGAAGCTTAC+TGG 0.304877 1.2:-8668289 MS.gene060923:CDS
GAGACCAGTGAGGACCCTTT+TGG 0.316595 1.2:-8669809 MS.gene060923:CDS
GGTAATTTCGGAACCATATA+TGG 0.326418 1.2:+8667608 None:intergenic
TGGTTTGGCGACACGTATAA+TGG 0.332505 1.2:+8666499 None:intergenic
GCTGAAGGAGATGCTGTTAC+AGG 0.334058 1.2:-8668194 MS.gene060923:intron
CTTACTGGGAACCACCTAAA+AGG 0.340476 1.2:-8668274 MS.gene060923:CDS
AAACACAAACCTGGTAATTT+CGG 0.347713 1.2:+8667596 None:intergenic
TGAGAATGAAGTTGAAGTTA+AGG 0.350478 1.2:-8669764 MS.gene060923:CDS
TCTTCGGTTGTTACCTGGTT+TGG 0.354472 1.2:+8666484 None:intergenic
TTCTCTTTGTTCCTGAATAC+AGG 0.355373 1.2:+8669727 None:intergenic
ATGGTGCTTCACCTGTATTC+AGG 0.357859 1.2:-8669738 MS.gene060923:CDS
GGAAAGGCTTGGCCTTTCTA+TGG 0.363818 1.2:+8667665 None:intergenic
GCTTCTGCCTCATTGCAAAA+AGG 0.366733 1.2:-8669157 MS.gene060923:CDS
AGGGTCCTCACTGGTCTCTC+TGG 0.383639 1.2:+8669814 None:intergenic
AATTTAACAGACGGGCCGTT+GGG 0.387269 1.2:+8668514 None:intergenic
CACATATCTGCGAACTCGTC+AGG 0.394150 1.2:+8666271 None:intergenic
CCGACATTTGATGTTGAACT+TGG 0.401652 1.2:-8669184 MS.gene060923:CDS
AGCTTTGCAGAAGAAGAAAA+AGG 0.403828 1.2:-8666365 MS.gene060923:CDS
CAGGAACAAAGAGAAAGTTT+TGG 0.404050 1.2:-8669719 MS.gene060923:CDS
AGCTGTGGCTGTGCCCAAAA+AGG 0.410300 1.2:-8669848 MS.gene060923:CDS
GGCTGGAACGTTTGCAAAGA+AGG 0.416450 1.2:-8666344 MS.gene060923:CDS
TTGGCCTTTCTATGGTCCTT+CGG 0.429083 1.2:+8667673 None:intergenic
GAGCTCAACAAGCTCGTTCA+AGG 0.438472 1.2:+8669098 None:intergenic
TCTTATCCTTCCATCCCAAA+AGG 0.448553 1.2:+8669794 None:intergenic
GCATGAGATGGCCAATCCTC+TGG 0.459429 1.2:-8666296 MS.gene060923:CDS
AGGCAAAAGATCTTTATCTC+TGG 0.470351 1.2:-8668549 MS.gene060923:CDS
GGTTGAATCGAAAGAAACTA+AGG 0.485204 1.2:-8669127 MS.gene060923:CDS
TTTGATGAAAATGTCAAAAG+AGG 0.486922 1.2:+8668244 None:intergenic
CTCGTCAGGTTCCAGAGGAT+TGG 0.494627 1.2:+8666285 None:intergenic
AATCCAATTAAAATATTTGG+TGG 0.497076 1.2:-8666553 MS.gene060923:CDS
AAATTTAACAGACGGGCCGT+TGG 0.517299 1.2:+8668513 None:intergenic
TATATGGTTCCGAAATTACC+AGG 0.519157 1.2:-8667605 MS.gene060923:intron
ATTATACGTGTCGCCAAACC+AGG 0.521192 1.2:-8666497 MS.gene060923:intron
ATGGATTCTCATATAAAGTC+GGG 0.523055 1.2:+8666518 None:intergenic
GAAGTTGAAGTTAAGGAGAA+TGG 0.527506 1.2:-8669757 MS.gene060923:CDS
CTTATCCTTCCATCCCAAAA+GGG 0.530204 1.2:+8669795 None:intergenic
TGATAAAATGACATTGATTG+AGG 0.544457 1.2:-8666680 MS.gene060923:intron
ATGAAAATGTCAAAAGAGGA+CGG 0.546674 1.2:+8668248 None:intergenic
CTGACGAGTTCGCAGATATG+TGG 0.547350 1.2:-8666270 MS.gene060923:CDS
CATCCCAAAAGGGTCCTCAC+TGG 0.551306 1.2:+8669805 None:intergenic
AATGGATTCTCATATAAAGT+CGG 0.552929 1.2:+8666517 None:intergenic
TGGAGGAATTGAAGCTTACT+GGG 0.555656 1.2:-8668288 MS.gene060923:CDS
GAAGAGAAAACGAAGCGAAA+AGG 0.560289 1.2:-8669875 MS.gene060923:CDS
AAAAGATCTTTATCTCTGGA+TGG 0.569362 1.2:-8668545 MS.gene060923:CDS
TGAAAATGTCAAAAGAGGAC+GGG 0.576450 1.2:+8668249 None:intergenic
ATTTAACAGACGGGCCGTTG+GGG 0.581078 1.2:+8668515 None:intergenic
AAGGAGGAAAATGCATGAGA+TGG 0.589573 1.2:-8666308 MS.gene060923:CDS
AACACCGAAGGACCATAGAA+AGG 0.600443 1.2:-8667677 MS.gene060923:CDS
AACATGGTCATGGAAAGGCT+TGG 0.604292 1.2:+8667654 None:intergenic
AAGCGAAAAGGCTGAAGCTG+TGG 0.609947 1.2:-8669863 MS.gene060923:CDS
TGCATACTGGAAATTGCTGA+AGG 0.612381 1.2:-8668209 MS.gene060923:CDS
AGAAGGTCAAAGCAAAGACA+AGG 0.616949 1.2:-8666327 MS.gene060923:CDS
TACATGTTTAGGTTGGTCCA+CGG 0.617665 1.2:-8666585 MS.gene060923:intron
TGGATGGCAAAATGCCCCAA+CGG 0.618112 1.2:-8668529 MS.gene060923:CDS
AATTTGTCTGACTCATTGTG+AGG 0.622655 1.2:+8666718 None:intergenic
GTTGAATCGAAAGAAACTAA+GGG 0.624547 1.2:-8669126 MS.gene060923:CDS
CTGCTTTCCAGTGCACACAA+TGG 0.626669 1.2:-8668308 MS.gene060923:intron
ATTGGATTCAAGCAGAACCG+TGG 0.628585 1.2:+8666568 None:intergenic
CTTTCCAGTGCACACAATGG+AGG 0.635931 1.2:-8668305 MS.gene060923:intron
AGGTCAAAGCAAAGACAAGG+AGG 0.640567 1.2:-8666324 MS.gene060923:CDS
GCGAACTCGTCAGGTTCCAG+AGG 0.642544 1.2:+8666280 None:intergenic
CCAAGTTCAACATCAAATGT+CGG 0.645758 1.2:+8669184 None:intergenic
ATGTGAATTTCAGTGCAGGA+AGG 0.659681 1.2:-8668569 MS.gene060923:intron
CCAGATATTTGAAACACCGA+AGG 0.677611 1.2:-8667689 MS.gene060923:intron
CTCAACAAGCTCGTTCAAGG+TGG 0.686995 1.2:+8669101 None:intergenic
TTGCTCCAGAGAGACCAGTG+AGG 0.749614 1.2:-8669819 MS.gene060923:CDS
GAGTCAGACAAATTACCCCG+AGG 0.769107 1.2:-8666709 MS.gene060923:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACAGCAAAATATATTT+GGG + chr1.2:8666491-8666510 None:intergenic 15.0%
!! AATACTATATAATCATAACA+AGG + chr1.2:8667717-8667736 None:intergenic 15.0%
!! AATTTAAATTGAGTAGAATT+TGG - chr1.2:8668242-8668261 MS.gene060923:CDS 15.0%
!! ATAATAATAATAGTATCATG+TGG + chr1.2:8669031-8669050 None:intergenic 15.0%
!! ATTTAAATTGAGTAGAATTT+GGG - chr1.2:8668243-8668262 MS.gene060923:CDS 15.0%
!! CATTATTTATATTTATCTAC+TGG + chr1.2:8666793-8666812 None:intergenic 15.0%
!! TTATGATTATATAGTATTCT+AGG - chr1.2:8667719-8667738 MS.gene060923:intron 15.0%
!! TTGAATCCAATTAAAATATT+TGG - chr1.2:8669585-8669604 MS.gene060923:intron 15.0%
!!! AGTTTATTAGTTTTTGAATA+TGG - chr1.2:8666908-8666927 MS.gene060923:intron 15.0%
!!! TATTATTTTTTATGACATCA+AGG - chr1.2:8667097-8667116 MS.gene060923:intron 15.0%
!! AAGTCTATAAATGTTATGTT+TGG - chr1.2:8668202-8668221 MS.gene060923:CDS 20.0%
!! AATACATAGAAAAACCAATT+TGG + chr1.2:8667412-8667431 None:intergenic 20.0%
!! AATCCAATTAAAATATTTGG+TGG - chr1.2:8669588-8669607 MS.gene060923:intron 20.0%
!! ACTCAAAAATAACTACATAT+TGG - chr1.2:8669717-8669736 MS.gene060923:CDS 20.0%
!! ATACATAGAAAAACCAATTT+GGG + chr1.2:8667411-8667430 None:intergenic 20.0%
!! ATTGATCATAAACTTATTAC+AGG - chr1.2:8666932-8666951 MS.gene060923:intron 20.0%
!! CAAATACAGCAAAATATATT+TGG + chr1.2:8666492-8666511 None:intergenic 20.0%
!! CAGATTAAATTAATGTCAAA+TGG - chr1.2:8666637-8666656 MS.gene060923:intron 20.0%
!! CTAACTAAAAATTTAACAGA+CGG + chr1.2:8667639-8667658 None:intergenic 20.0%
!! GAAAATTCATTGTTTGTTTA+TGG - chr1.2:8667179-8667198 MS.gene060923:intron 20.0%
!! TAAAAAATAGAATTCAGAAC+TGG + chr1.2:8667754-8667773 None:intergenic 20.0%
!! TAAAAGAGAAACTAGTAAAA+TGG + chr1.2:8667807-8667826 None:intergenic 20.0%
!! TAACTAAAAATTTAACAGAC+GGG + chr1.2:8667638-8667657 None:intergenic 20.0%
!! TATAATCATAACAAGGAAAT+TGG + chr1.2:8667710-8667729 None:intergenic 20.0%
!! TTCACTCAATTCAAAAATTA+TGG + chr1.2:8668737-8668756 None:intergenic 20.0%
!!! CAGTTTACTTATTTTAAATC+TGG - chr1.2:8667436-8667455 MS.gene060923:intron 20.0%
!!! CGACACTTTAAAATTTAAAA+TGG + chr1.2:8667362-8667381 None:intergenic 20.0%
!!! GTAATATGATTGTTTTACTA+TGG - chr1.2:8668971-8668990 MS.gene060923:intron 20.0%
!!! TATAATTATGCCAATTGTTT+TGG - chr1.2:8668707-8668726 MS.gene060923:intron 20.0%
!!! TCATGATATCATATTTCTAT+TGG + chr1.2:8668895-8668914 None:intergenic 20.0%
! AACAACTTAACTAAGTTGTT+AGG + chr1.2:8666566-8666585 None:intergenic 25.0%
! AACTAATAAACTGCAATAAC+AGG + chr1.2:8666900-8666919 None:intergenic 25.0%
! AGGAAACTGAAAATAGTTTA+TGG - chr1.2:8666712-8666731 MS.gene060923:CDS 25.0%
! ATAACCAAAATTAAGTTCAC+TGG + chr1.2:8669224-8669243 None:intergenic 25.0%
! CAATTGAGAAAACAAAAACA+TGG + chr1.2:8668506-8668525 None:intergenic 25.0%
! CTAATGCAAAATGTTATTGT+TGG - chr1.2:8667258-8667277 MS.gene060923:intron 25.0%
! CTGAGTTATGTATTTGAATA+GGG - chr1.2:8668315-8668334 MS.gene060923:intron 25.0%
! GTTGTATTCATAAACTATCT+TGG - chr1.2:8666678-8666697 MS.gene060923:intron 25.0%
! TCAATAAAACCATCTTAGTT+TGG - chr1.2:8668026-8668045 MS.gene060923:intron 25.0%
! TCTATTCTATTTGTGACATT+TGG - chr1.2:8667522-8667541 MS.gene060923:intron 25.0%
! TGAGTTATGTATTTGAATAG+GGG - chr1.2:8668316-8668335 MS.gene060923:intron 25.0%
! TTGCAAAGGATCTAAATAAT+TGG - chr1.2:8668572-8668591 MS.gene060923:intron 25.0%
! TTTGATGAAAATGTCAAAAG+AGG + chr1.2:8667900-8667919 None:intergenic 25.0%
!! AATGGATTCTCATATAAAGT+CGG + chr1.2:8669627-8669646 None:intergenic 25.0%
!! ACATCAGTTAAACGAATTTT+AGG + chr1.2:8669528-8669547 None:intergenic 25.0%
!! AGTTAAGTTGTTATGTTGTA+AGG - chr1.2:8666574-8666593 MS.gene060923:CDS 25.0%
!! GTTAAGTTGTTATGTTGTAA+GGG - chr1.2:8666575-8666594 MS.gene060923:CDS 25.0%
!! TAATCCAGTGAACTTAATTT+TGG - chr1.2:8669217-8669236 MS.gene060923:intron 25.0%
!! TGATAAAATGACATTGATTG+AGG - chr1.2:8669461-8669480 MS.gene060923:intron 25.0%
!!! ATTGTTTTACTATGGTTACA+TGG - chr1.2:8668979-8668998 MS.gene060923:intron 25.0%
!!! ATTTTGAAAAAGATGCATAC+TGG - chr1.2:8667919-8667938 MS.gene060923:intron 25.0%
!!! CAAAATATATTTGGGTTTTG+TGG + chr1.2:8666483-8666502 None:intergenic 25.0%
!!! CAATCAATGTCATTTTATCA+AGG + chr1.2:8669461-8669480 None:intergenic 25.0%
!!! GAGTTTGGATTTGTTTATTT+TGG - chr1.2:8666531-8666550 MS.gene060923:CDS 25.0%
!!! GTTAAATTTTTAGTTAGTGC+CGG - chr1.2:8667642-8667661 MS.gene060923:CDS 25.0%
!!! GTTGATAGTATATAAGGTTT+TGG + chr1.2:8668945-8668964 None:intergenic 25.0%
!!! TAAACAATGCTATTTTATGC+TGG - chr1.2:8669272-8669291 MS.gene060923:intron 25.0%
!!! TGTTTTGGATTCAAAAGTTA+AGG - chr1.2:8667998-8668017 MS.gene060923:intron 25.0%
!!! TTCTTCGTGTTTATTTTTTG+AGG - chr1.2:8667074-8667093 MS.gene060923:intron 25.0%
!!! TTCTTCGTTGATAGTATATA+AGG + chr1.2:8668951-8668970 None:intergenic 25.0%
AAAAGATCTTTATCTCTGGA+TGG - chr1.2:8667596-8667615 MS.gene060923:intron 30.0%
AAACACAAACCTGGTAATTT+CGG + chr1.2:8668548-8668567 None:intergenic 30.0%
AAACTATCTTGGAGAACTTA+TGG - chr1.2:8666689-8666708 MS.gene060923:CDS 30.0%
AGAAAACAAAAACATGGTCA+TGG + chr1.2:8668500-8668519 None:intergenic 30.0%
ATAGAAAAACCAATTTGGGA+GGG + chr1.2:8667407-8667426 None:intergenic 30.0%
ATGAAAATGTCAAAAGAGGA+CGG + chr1.2:8667896-8667915 None:intergenic 30.0%
ATGGATTCTCATATAAAGTC+GGG + chr1.2:8669626-8669645 None:intergenic 30.0%
CAAACAGATGAGAATATTAC+CGG + chr1.2:8667664-8667683 None:intergenic 30.0%
CCTATTCAAATACATAACTC+AGG + chr1.2:8668317-8668336 None:intergenic 30.0%
CCTGAGTTATGTATTTGAAT+AGG - chr1.2:8668314-8668333 MS.gene060923:intron 30.0%
GGATCTACAACAACATAAAA+AGG + chr1.2:8669755-8669774 None:intergenic 30.0%
GTTGAATCGAAAGAAACTAA+GGG - chr1.2:8667015-8667034 MS.gene060923:intron 30.0%
GTTTAAACTTCAAAATCCGT+TGG - chr1.2:8668629-8668648 MS.gene060923:intron 30.0%
TCATTGCAAAAAGGATAACA+AGG - chr1.2:8666993-8667012 MS.gene060923:intron 30.0%
TCTGCATGAGAATAAAAATG+TGG + chr1.2:8669395-8669414 None:intergenic 30.0%
TGAGAATGAAGTTGAAGTTA+AGG - chr1.2:8666377-8666396 MS.gene060923:intron 30.0%
TGTTGAAAACAACAAACCAA+CGG + chr1.2:8668648-8668667 None:intergenic 30.0%
TTATAGACTTCTCAAGTCAA+CGG + chr1.2:8668192-8668211 None:intergenic 30.0%
TTATGGCTAACCAAAACAAT+TGG + chr1.2:8668720-8668739 None:intergenic 30.0%
! AACATTGCTGATGGATAAAT+TGG - chr1.2:8668794-8668813 MS.gene060923:intron 30.0%
! ACATTGCTGATGGATAAATT+GGG - chr1.2:8668795-8668814 MS.gene060923:intron 30.0%
! ATAAAACCATCTTAGTTTGG+TGG - chr1.2:8668029-8668048 MS.gene060923:intron 30.0%
! GTTGACTTATGCAAAGAAAA+TGG - chr1.2:8669153-8669172 MS.gene060923:CDS 30.0%
! GTTTGTGTTTTCATTTGCAA+AGG - chr1.2:8668558-8668577 MS.gene060923:CDS 30.0%
! TTGTTATCCTTTTTGCAATG+AGG + chr1.2:8666994-8667013 None:intergenic 30.0%
! TTTTCCATTTAATCTCGAGT+TGG - chr1.2:8667473-8667492 MS.gene060923:intron 30.0%
!! AAAATATTTGGTGGCAGTTT+TGG - chr1.2:8669597-8669616 MS.gene060923:intron 30.0%
!! CAATTGTTGATGACCATATA+TGG - chr1.2:8668520-8668539 MS.gene060923:CDS 30.0%
!! CTCATCTGTTTGTTATCTTA+AGG - chr1.2:8667672-8667691 MS.gene060923:CDS 30.0%
!! TTCACAATTTTCTCTGGTTT+TGG - chr1.2:8668283-8668302 MS.gene060923:CDS 30.0%
!!! ATCTCTTTCCTTTTTTGTGA+TGG - chr1.2:8667296-8667315 MS.gene060923:intron 30.0%
!!! CTGCCACCAAATATTTTAAT+TGG + chr1.2:8669594-8669613 None:intergenic 30.0%
!!! GTGCTTTTTTCTTTCTTTCT+TGG - chr1.2:8668365-8668384 MS.gene060923:intron 30.0%
AAAAATAAGCGCGAGAATCT+TGG - chr1.2:8668683-8668702 MS.gene060923:intron 35.0%
AACCAGAGAAAATTGTGAAC+AGG + chr1.2:8668282-8668301 None:intergenic 35.0%
AACTCCAACTCGAGATTAAA+TGG + chr1.2:8667480-8667499 None:intergenic 35.0%
AATTTGTCTGACTCATTGTG+AGG + chr1.2:8669426-8669445 None:intergenic 35.0%
ACAAAAACATGGTCATGGAA+AGG + chr1.2:8668495-8668514 None:intergenic 35.0%
AGAAACTAGTAAAATGGCCT+CGG + chr1.2:8667801-8667820 None:intergenic 35.0%
AGCAATGTTAGCAAAGAAAG+GGG + chr1.2:8668783-8668802 None:intergenic 35.0%
AGCTTTGCAGAAGAAGAAAA+AGG - chr1.2:8669776-8669795 MS.gene060923:CDS 35.0%
AGGCAAAAGATCTTTATCTC+TGG - chr1.2:8667592-8667611 MS.gene060923:intron 35.0%
ATTGATTGAGGTGTGCAAAT+TGG - chr1.2:8669473-8669492 MS.gene060923:intron 35.0%
CAGCAATGTTAGCAAAGAAA+GGG + chr1.2:8668784-8668803 None:intergenic 35.0%
CATAAGTCAACAATCACATG+TGG + chr1.2:8669144-8669163 None:intergenic 35.0%
CATAGAAAAACCAATTTGGG+AGG + chr1.2:8667408-8667427 None:intergenic 35.0%
CCAAGTTCAACATCAAATGT+CGG + chr1.2:8666960-8666979 None:intergenic 35.0%
GAAGTTGAAGTTAAGGAGAA+TGG - chr1.2:8666384-8666403 MS.gene060923:intron 35.0%
GATGTGATCCATCACAAAAA+AGG + chr1.2:8667307-8667326 None:intergenic 35.0%
GCAAAGAAAATGGATCTTCA+GGG - chr1.2:8669163-8669182 MS.gene060923:CDS 35.0%
GCATATCTTAAACACCATGA+CGG - chr1.2:8669116-8669135 MS.gene060923:CDS 35.0%
GGTAATTTCGGAACCATATA+TGG + chr1.2:8668536-8668555 None:intergenic 35.0%
GGTTGAATCGAAAGAAACTA+AGG - chr1.2:8667014-8667033 MS.gene060923:intron 35.0%
GTGTACATTGAGAACATCTT+CGG + chr1.2:8669676-8669695 None:intergenic 35.0%
TAAACACTTGTGAGACTGTT+TGG + chr1.2:8666768-8666787 None:intergenic 35.0%
TAGAAAAACCAATTTGGGAG+GGG + chr1.2:8667406-8667425 None:intergenic 35.0%
TATATGGTTCCGAAATTACC+AGG - chr1.2:8668536-8668555 MS.gene060923:CDS 35.0%
TCAGCAATGTTAGCAAAGAA+AGG + chr1.2:8668785-8668804 None:intergenic 35.0%
TGAAAATGTCAAAAGAGGAC+GGG + chr1.2:8667895-8667914 None:intergenic 35.0%
TGCAAACTACATGTTTAGGT+TGG - chr1.2:8669549-8669568 MS.gene060923:intron 35.0%
TGCAAAGAAAATGGATCTTC+AGG - chr1.2:8669162-8669181 MS.gene060923:CDS 35.0%
TGCAAATGAAAACACAAACC+TGG + chr1.2:8668557-8668576 None:intergenic 35.0%
TGTGAACAGGAATAAATGCT+TGG + chr1.2:8668269-8668288 None:intergenic 35.0%
TGTGTGCAAACTACATGTTT+AGG - chr1.2:8669545-8669564 MS.gene060923:intron 35.0%
TTCTCTTTGTTCCTGAATAC+AGG + chr1.2:8666417-8666436 None:intergenic 35.0%
TTCTTTGCTAACATTGCTGA+TGG - chr1.2:8668785-8668804 MS.gene060923:intron 35.0%
! AGTTCTTTTACTGCACACAA+AGG + chr1.2:8666830-8666849 None:intergenic 35.0%
! CAGGAACAAAGAGAAAGTTT+TGG - chr1.2:8666422-8666441 MS.gene060923:intron 35.0%
! GTTCTTTTACTGCACACAAA+GGG + chr1.2:8666829-8666848 None:intergenic 35.0%
! TACTCTTCTATGCACTGTTT+TGG - chr1.2:8667983-8668002 MS.gene060923:intron 35.0%
! TCATTTTATCAAGGCTTCCT+CGG + chr1.2:8669452-8669471 None:intergenic 35.0%
! TTCCTGTTCACAATTTTCTC+TGG - chr1.2:8668277-8668296 MS.gene060923:CDS 35.0%
! TTTTCTTCTTCTGCAAAGCT+CGG + chr1.2:8669776-8669795 None:intergenic 35.0%
!!! AAGTTTTGGTTACTTGCTCT+AGG - chr1.2:8666436-8666455 MS.gene060923:intron 35.0%
!!! ACTTGCATTTTTTGCGAATG+TGG - chr1.2:8669298-8669317 MS.gene060923:intron 35.0%
!!! GTTTGTGCTTGTTTTGAGTT+TGG - chr1.2:8666516-8666535 MS.gene060923:CDS 35.0%
AAGGAGGAAAATGCATGAGA+TGG - chr1.2:8669833-8669852 MS.gene060923:CDS 40.0%
ACTTGCTCGAGATTAAGAAC+TGG + chr1.2:8667503-8667522 None:intergenic 40.0%
AGAAGGTCAAAGCAAAGACA+AGG - chr1.2:8669814-8669833 MS.gene060923:CDS 40.0%
ATGGAGGAATTGAAGCTTAC+TGG - chr1.2:8667852-8667871 MS.gene060923:intron 40.0%
ATGTGAATTTCAGTGCAGGA+AGG - chr1.2:8667572-8667591 MS.gene060923:intron 40.0%
CCGACATTTGATGTTGAACT+TGG - chr1.2:8666957-8666976 MS.gene060923:intron 40.0%
CTATCTTGGAGAACTTATGG+AGG - chr1.2:8666692-8666711 MS.gene060923:CDS 40.0%
CTGAAATTCACATGCACAGA+AGG + chr1.2:8667565-8667584 None:intergenic 40.0%
CTTATCCTTCCATCCCAAAA+GGG + chr1.2:8666349-8666368 None:intergenic 40.0%
GAAGAGAAAACGAAGCGAAA+AGG - chr1.2:8666266-8666285 MS.gene060923:CDS 40.0%
GAGATGCTGTTACAGGTATT+TGG - chr1.2:8667954-8667973 MS.gene060923:intron 40.0%
TACATCGTCTACACTTGTCT+AGG - chr1.2:8669062-8669081 MS.gene060923:intron 40.0%
TATGTATTTGAATAGGGGCG+TGG - chr1.2:8668321-8668340 MS.gene060923:intron 40.0%
TCTTATCCTTCCATCCCAAA+AGG + chr1.2:8666350-8666369 None:intergenic 40.0%
TGCATACTGGAAATTGCTGA+AGG - chr1.2:8667932-8667951 MS.gene060923:intron 40.0%
TGGAGGAATTGAAGCTTACT+GGG - chr1.2:8667853-8667872 MS.gene060923:intron 40.0%
TTGCAGAAGAAGAAAAAGGC+TGG - chr1.2:8669780-8669799 MS.gene060923:CDS 40.0%
! AAAGCACCACCAAACTAAGA+TGG + chr1.2:8668038-8668057 None:intergenic 40.0%
! ATTTTATCAAGGCTTCCTCG+GGG + chr1.2:8669450-8669469 None:intergenic 40.0%
! CATTTTATCAAGGCTTCCTC+GGG + chr1.2:8669451-8669470 None:intergenic 40.0%
! CCAGATATTTGAAACACCGA+AGG - chr1.2:8668452-8668471 MS.gene060923:intron 40.0%
! GGAGCAACATCTTCCTTTTT+GGG + chr1.2:8666309-8666328 None:intergenic 40.0%
! TGGAGCAACATCTTCCTTTT+TGG + chr1.2:8666310-8666329 None:intergenic 40.0%
!! ATATTTGGTGGCAGTTTTGG+AGG - chr1.2:8669600-8669619 MS.gene060923:intron 40.0%
!! ATCTTAGTTTGGTGGTGCTT+TGG - chr1.2:8668037-8668056 MS.gene060923:intron 40.0%
!! CCTTCGGTGTTTCAAATATC+TGG + chr1.2:8668455-8668474 None:intergenic 40.0%
!! TACATGTTTAGGTTGGTCCA+CGG - chr1.2:8669556-8669575 MS.gene060923:intron 40.0%
!! TCTTAGTTTGGTGGTGCTTT+GGG - chr1.2:8668038-8668057 MS.gene060923:intron 40.0%
AAATTTAACAGACGGGCCGT+TGG + chr1.2:8667631-8667650 None:intergenic 45.0%
AACACCGAAGGACCATAGAA+AGG - chr1.2:8668464-8668483 MS.gene060923:intron 45.0%
AACATGGTCATGGAAAGGCT+TGG + chr1.2:8668490-8668509 None:intergenic 45.0%
AATCACATGTGGTACCGTCA+TGG + chr1.2:8669133-8669152 None:intergenic 45.0%
AATTCCTCCATTGTGTGCAC+TGG + chr1.2:8667843-8667862 None:intergenic 45.0%
AATTTAACAGACGGGCCGTT+GGG + chr1.2:8667630-8667649 None:intergenic 45.0%
AGGTCAAAGCAAAGACAAGG+AGG - chr1.2:8669817-8669836 MS.gene060923:CDS 45.0%
ATGGTGCTTCACCTGTATTC+AGG - chr1.2:8666403-8666422 MS.gene060923:intron 45.0%
ATTATACGTGTCGCCAAACC+AGG - chr1.2:8669644-8669663 MS.gene060923:intron 45.0%
ATTGGATTCAAGCAGAACCG+TGG + chr1.2:8669576-8669595 None:intergenic 45.0%
CAATTTGGGAGGGGAAACAA+AGG + chr1.2:8667397-8667416 None:intergenic 45.0%
CTTACTGGGAACCACCTAAA+AGG - chr1.2:8667867-8667886 MS.gene060923:intron 45.0%
GAACATCTTCGGTTGTTACC+TGG + chr1.2:8669665-8669684 None:intergenic 45.0%
GCTTCTGCCTCATTGCAAAA+AGG - chr1.2:8666984-8667003 MS.gene060923:intron 45.0%
GTGCATGTGAATTTCAGTGC+AGG - chr1.2:8667568-8667587 MS.gene060923:intron 45.0%
TCTTCGGTTGTTACCTGGTT+TGG + chr1.2:8669660-8669679 None:intergenic 45.0%
! CTTTGTTTCCCCTCCCAAAT+TGG - chr1.2:8667395-8667414 MS.gene060923:intron 45.0%
! GCTCTAGGCGTATTGTTTAC+AGG - chr1.2:8666451-8666470 MS.gene060923:intron 45.0%
! TGGTTTGGCGACACGTATAA+TGG + chr1.2:8669645-8669664 None:intergenic 45.0%
! TTGGCCTTTCTATGGTCCTT+CGG + chr1.2:8668471-8668490 None:intergenic 45.0%
!! CTTAGTTTGGTGGTGCTTTG+GGG - chr1.2:8668039-8668058 MS.gene060923:intron 45.0%
!! TTAGTTTGGTGGTGCTTTGG+GGG - chr1.2:8668040-8668059 MS.gene060923:intron 45.0%
AAGCGAAAAGGCTGAAGCTG+TGG - chr1.2:8666278-8666297 MS.gene060923:CDS 50.0%
ACTGTCTCTCTTTCTCTCCG+AGG - chr1.2:8667781-8667800 MS.gene060923:intron 50.0%
ATTTAACAGACGGGCCGTTG+GGG + chr1.2:8667629-8667648 None:intergenic 50.0%
CACATATCTGCGAACTCGTC+AGG + chr1.2:8669873-8669892 None:intergenic 50.0%
CTCAACAAGCTCGTTCAAGG+TGG + chr1.2:8667043-8667062 None:intergenic 50.0%
CTGACGAGTTCGCAGATATG+TGG - chr1.2:8669871-8669890 MS.gene060923:CDS 50.0%
CTGCTTTCCAGTGCACACAA+TGG - chr1.2:8667833-8667852 MS.gene060923:intron 50.0%
CTTTCCAGTGCACACAATGG+AGG - chr1.2:8667836-8667855 MS.gene060923:intron 50.0%
GAGCTCAACAAGCTCGTTCA+AGG + chr1.2:8667046-8667065 None:intergenic 50.0%
GAGTCAGACAAATTACCCCG+AGG - chr1.2:8669432-8669451 MS.gene060923:intron 50.0%
GCTGAAGGAGATGCTGTTAC+AGG - chr1.2:8667947-8667966 MS.gene060923:intron 50.0%
GGCTGGAACGTTTGCAAAGA+AGG - chr1.2:8669797-8669816 MS.gene060923:CDS 50.0%
TGGATGGCAAAATGCCCCAA+CGG - chr1.2:8667612-8667631 MS.gene060923:CDS 50.0%
TGTGCACTGGAAAGCAGTGA+AGG + chr1.2:8667830-8667849 None:intergenic 50.0%
! AAAGAGGACGGGAGCCTTTT+AGG + chr1.2:8667884-8667903 None:intergenic 50.0%
! AGACCAGTGAGGACCCTTTT+GGG - chr1.2:8666333-8666352 MS.gene060923:CDS 50.0%
! CTGCACACAAAGGGCTTGTT+TGG + chr1.2:8666820-8666839 None:intergenic 50.0%
! GGAAAGGCTTGGCCTTTCTA+TGG + chr1.2:8668479-8668498 None:intergenic 50.0%
AGCTGTGGCTGTGCCCAAAA+AGG - chr1.2:8666293-8666312 MS.gene060923:CDS 55.0%
CATCCCAAAAGGGTCCTCAC+TGG + chr1.2:8666339-8666358 None:intergenic 55.0%
CTCGTCAGGTTCCAGAGGAT+TGG + chr1.2:8669859-8669878 None:intergenic 55.0%
GCATGAGATGGCCAATCCTC+TGG - chr1.2:8669845-8669864 MS.gene060923:CDS 55.0%
TTGCTCCAGAGAGACCAGTG+AGG - chr1.2:8666322-8666341 MS.gene060923:CDS 55.0%
! GAGACCAGTGAGGACCCTTT+TGG - chr1.2:8666332-8666351 MS.gene060923:CDS 55.0%
!! CAGTGAGGACCCTTTTGGGA+TGG - chr1.2:8666337-8666356 MS.gene060923:CDS 55.0%
!!! GAGGACCCTTTTGGGATGGA+AGG - chr1.2:8666341-8666360 MS.gene060923:CDS 55.0%
GCGAACTCGTCAGGTTCCAG+AGG + chr1.2:8669864-8669883 None:intergenic 60.0%
! AGGGTCCTCACTGGTCTCTC+TGG + chr1.2:8666330-8666349 None:intergenic 60.0%
! GAGGACGGGAGCCTTTTAGG+TGG + chr1.2:8667881-8667900 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 8666261 8669902 8666261 ID=MS.gene060923
chr1.2 mRNA 8666261 8669902 8666261 ID=MS.gene060923.t1;Parent=MS.gene060923
chr1.2 exon 8669691 8669902 8669691 ID=MS.gene060923.t1.exon1;Parent=MS.gene060923.t1
chr1.2 CDS 8669691 8669902 8669691 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8669068 8669209 8669068 ID=MS.gene060923.t1.exon2;Parent=MS.gene060923.t1
chr1.2 CDS 8669068 8669209 8669068 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8668500 8668578 8668500 ID=MS.gene060923.t1.exon3;Parent=MS.gene060923.t1
chr1.2 CDS 8668500 8668578 8668500 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8668195 8668319 8668195 ID=MS.gene060923.t1.exon4;Parent=MS.gene060923.t1
chr1.2 CDS 8668195 8668319 8668195 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8667606 8667707 8667606 ID=MS.gene060923.t1.exon5;Parent=MS.gene060923.t1
chr1.2 CDS 8667606 8667707 8667606 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8666681 8666749 8666681 ID=MS.gene060923.t1.exon6;Parent=MS.gene060923.t1
chr1.2 CDS 8666681 8666749 8666681 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8666498 8666596 8666498 ID=MS.gene060923.t1.exon7;Parent=MS.gene060923.t1
chr1.2 CDS 8666498 8666596 8666498 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
chr1.2 exon 8666261 8666392 8666261 ID=MS.gene060923.t1.exon8;Parent=MS.gene060923.t1
chr1.2 CDS 8666261 8666392 8666261 ID=cds.MS.gene060923.t1;Parent=MS.gene060923.t1
Gene Sequence

>MS.gene060923

ATGGGGAAGAGAAAACGAAGCGAAAAGGCTGAAGCTGTGGCTGTGCCCAAAAAGGAAGATGTTGCTCCAGAGAGACCAGTGAGGACCCTTTTGGGATGGAAGGATAAGAATGAAAATGAGAATGAAGTTGAAGTTAAGGAGAATGGTGCTTCACCTGTATTCAGGAACAAAGAGAAAGTTTTGGTTACTTGCTCTAGGCGTATTGTTTACAGGTACAAACCACAAAACCCAAATATATTTTGCTGTATTTGCGCAGTTTGTGCTTGTTTTGAGTTTGGATTTGTTTATTTTGGCTATGTTTGCCTAACAACTTAGTTAAGTTGTTATGTTGTAAGGGTTTATCATGTAAGTGCTTATTTATAAGTTATTTCTACAACAGATTAAATTAATGTCAAATGGTTTTGATGTAAGTTATAAGTTGTATTCATAAACTATCTTGGAGAACTTATGGAGGAAACTGAAAATAGTTTATGGACAAGTCATTTGTTGTTTCTAGAAGCTGTTCCAAACAGTCTCACAAGTGTTTATTCCAGTAGATAAATATAAATAATGTAATCCAAACAAGCCCTTTGTGTGCAGTAAAAGAACTGTATCAGAATGCTCTTGTCTCTGTGATATTAAGCACACAAACTTCTTCCTGTTATTGCAGTTTATTAGTTTTTGAATATGGTATTGATCATAAACTTATTACAGGTACCGACATTTGATGTTGAACTTGGTATCGCTTCTGCCTCATTGCAAAAAGGATAACAAGGTTGAATCGAAAGAAACTAAGGGCGCCACCTTGAACGAGCTTGTTGAGCTCAAAAACTGTTCTTCGTGTTTATTTTTTGAGGTATTATTTTTTATGACATCAAGGTTTTTTCAATGACAACTATGCATTATGTTTATGAGCTATGCTATGTTGTTGTTGAGTTAGAAAATTCATTGTTTGTTTATGGCGTGAAGAATTATTCGATTTTGTTACAAATGTTGATGTCTATTTTGATATTTAGATCTAATGCAAAATGTTATTGTTGGTTACAAATTGATATAATCTCTTTCCTTTTTTGTGATGGATCACATCTGATTCTTGTGTCTTATAGATTTTTATTTTATCCATTTTAAATTTTAAAGTGTCGAACACTTTCACTCCTTTGTTTCCCCTCCCAAATTGGTTTTTCTATGTATTTACGCAGTTTACTTATTTTAAATCTGGCGACTGAGACAACATTTTCCATTTAATCTCGAGTTGGAGTTCCAGTTCTTAATCTCGAGCAAGTCTATTCTATTTGTGACATTTGGATAACTATCTATATCTACCTTCTGTGCATGTGAATTTCAGTGCAGGAAGGCAAAAGATCTTTATCTCTGGATGGCAAAATGCCCCAACGGCCCGTCTGTTAAATTTTTAGTTAGTGCCGGTAATATTCTCATCTGTTTGTTATCTTAAGGTTACTACATAAACCAATTTCCTTGTTATGATTATATAGTATTCTAGGATCAGTAATCCAGTTCTGAATTCTATTTTTTAAATTATGACTGTCTCTCTTTCTCTCCGAGGCCATTTTACTAGTTTCTCTTTTACCTTCACTGCTTTCCAGTGCACACAATGGAGGAATTGAAGCTTACTGGGAACCACCTAAAAGGCTCCCGTCCTCTTTTGACATTTTCATCAAATTTTGAAAAAGATGCATACTGGAAATTGCTGAAGGAGATGCTGTTACAGGTATTTGGTTTGATTACTCTTCTATGCACTGTTTTGGATTCAAAAGTTAAGGTCATTTCAATAAAACCATCTTAGTTTGGTGGTGCTTTGGGGGTGTTGCGTTGTATGAGTTACTAGTATGATTGAAAGTTGTTTTATGTGCACTTCTCTGACGTATTTGTGATCTTATAGTACAATTGTCATACAGTATCTATATGCTTAAAAGTTTCTTAATCTGTCACCGTTGACTTGAGAAGTCTATAAATGTTATGTTTGGCTCTTGTCATAGTTTAAAATTTAAATTGAGTAGAATTTGGGCCAAGCATTTATTCCTGTTCACAATTTTCTCTGGTTTTGGTTCTTGTACCTGAGTTATGTATTTGAATAGGGGCGTGGATTGCACGTGTGTGTGCTCGTGTGCTTTTTTCTTTCTTTCTTGGTTATTAGAATAATCAAAGTATACACAATTTTGATTATTGCTGACGTAGTCTTTCTTTTCTTCATCCAGATATTTGAAACACCGAAGGACCATAGAAAGGCCAAGCCTTTCCATGACCATGTTTTTGTTTTCTCAATTGTTGATGACCATATATGGTTCCGAAATTACCAGGTTTGTGTTTTCATTTGCAAAGGATCTAAATAATTGGCATAACATTGTTTTCAAATTTGTATTATTTTATAGTTTAAACTTCAAAATCCGTTGGTTTGTTGTTTTCAACATCACTCTACTAAAAAAAAAATAAGCGCGAGAATCTTGGTTATAATTATGCCAATTGTTTTGGTTAGCCATAATTTTTGAATTGAGTGAAATTGAGATGACAAATCTAAATTTCCCCTTTCTTTGCTAACATTGCTGATGGATAAATTGGGTTTGTGAGCATTTCAAATTTTTAAAGCATTAGTATATGAAATAAAAATATACTTCAACACAATTACTTTGAAGACCAATAGAAATATGATATCATGATGACTCTAGATTTGATGTAAAATCAAACCAAAACCTTATATACTATCAACGAAGAAGTAATATGATTGTTTTACTATGGTTACATGGAAAGCTTTCAACTAATTTTTTGTAAACCACATGATACTATTATTATTATTATTATGTAATTACATCGTCTACACTTGTCTAGGAGCAAAGAAAATGCATTCTTCACATAGATATGCATATCTTAAACACCATGACGGTACCACATGTGATTGTTGACTTATGCAAAGAAAATGGATCTTCAGGGTTACTTATTCATGCATTGTTGACTTTTATTCTAATCCAGTGAACTTAATTTTGGTTATTTTACAATGTATAGATGTCTTAATAATTTAAACAATGCTATTTTATGCTGGTACACTTGCATTTTTTGCGAATGTGGCTTTGCAATGTTTTTCTCATTTTCTGCTTTATGTTGTCATTATTGCTTACTGAACAATGTTACATAATAAACCACATTTTTATTCTCATGCAGATCTCTGTTCCTCACAATGAGTCAGACAAATTACCCCGAGGAAGCCTTGATAAAATGACATTGATTGAGGTGTGCAAATTGGCTTGTCAATTTATATTTGATCTGTCGATTCCTAAAATTCGTTTAACTGATGTGTGCAAACTACATGTTTAGGTTGGTCCACGGTTCTGCTTGAATCCAATTAAAATATTTGGTGGCAGTTTTGGAGGCCCGACTTTATATGAGAATCCATTATACGTGTCGCCAAACCAGGTAACAACCGAAGATGTTCTCAATGTACACTTCTAATTTCTGTAATTTTCTACTCAAAAATAACTACATATTGGTTATGTTTTTAACCTTTTTATGTTGTTGTAGATCCGAGCTTTGCAGAAGAAGAAAAAGGCTGGAACGTTTGCAAAGAAGGTCAAAGCAAAGACAAGGAGGAAAATGCATGAGATGGCCAATCCTCTGGAACCTGACGAGTTCGCAGATATGTGGAAAGATTAA
Protein sequence

>MS.gene060923.t1

MGKRKRSEKAEAVAVPKKEDVAPERPVRTLLGWKDKNENENEVEVKENGASPVFRNKEKVLVTCSRRIVYRYRHLMLNLVSLLPHCKKDNKVESKETKGATLNELVELKNCSSCLFFECRKAKDLYLWMAKCPNGPSVKFLVSAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAYWKLLKEMLLQIFETPKDHRKAKPFHDHVFVFSIVDDHIWFRNYQISVPHNESDKLPRGSLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALQKKKKAGTFAKKVKAKTRRKMHEMANPLEPDEFADMWKD