AlfalfaGEDB | MS.gene67316

M. sativa cultivar XinJiangDaYe / MS.gene67316

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67316.t1	KEH37197.1	84.3	70	7	1	1	66	1	70	2.10E-22	114.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67316.t1	A0A072V5T5	84.3	70	7	1	1	66	1	70	1.5e-22	114.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene67316	0.800176	1.63E-48	-1.69E-46
MS.gene049669	MS.gene67316	0.81023	1.28E-50	-1.69E-46
MS.gene050105	MS.gene67316	0.806622	7.52E-50	-1.69E-46
MS.gene050226	MS.gene67316	0.816731	4.75E-52	-1.69E-46
MS.gene050227	MS.gene67316	0.816397	5.65E-52	-1.69E-46
MS.gene050228	MS.gene67316	0.817891	2.61E-52	-1.69E-46
MS.gene050442	MS.gene67316	0.811561	6.58E-51	-1.69E-46
MS.gene050605	MS.gene67316	0.810283	1.25E-50	-1.69E-46
MS.gene050623	MS.gene67316	-0.811313	7.45E-51	-1.69E-46
MS.gene050642	MS.gene67316	0.815461	9.14E-52	-1.69E-46
MS.gene050680	MS.gene67316	0.839208	1.82E-57	-1.69E-46
MS.gene050750	MS.gene67316	0.820982	5.14E-53	-1.69E-46
MS.gene050905	MS.gene67316	-0.811794	5.86E-51	-1.69E-46
MS.gene050953	MS.gene67316	0.834195	3.45E-56	-1.69E-46
MS.gene051368	MS.gene67316	0.843485	1.36E-58	-1.69E-46
MS.gene051370	MS.gene67316	0.825084	5.68E-54	-1.69E-46
MS.gene051373	MS.gene67316	0.801814	7.54E-49	-1.69E-46
MS.gene051983	MS.gene67316	0.83025	3.27E-55	-1.69E-46
MS.gene052176	MS.gene67316	0.83053	2.79E-55	-1.69E-46
MS.gene052309	MS.gene67316	-0.828586	8.28E-55	-1.69E-46
MS.gene052333	MS.gene67316	0.830316	3.15E-55	-1.69E-46
MS.gene052426	MS.gene67316	0.831782	1.37E-55	-1.69E-46
MS.gene052867	MS.gene67316	0.813867	2.06E-51	-1.69E-46
MS.gene052954	MS.gene67316	0.808243	3.41E-50	-1.69E-46
MS.gene053006	MS.gene67316	0.801547	8.55E-49	-1.69E-46
MS.gene053166	MS.gene67316	0.844669	6.55E-59	-1.69E-46
MS.gene05415	MS.gene67316	0.803813	2.91E-49	-1.69E-46
MS.gene054291	MS.gene67316	-0.811095	8.31E-51	-1.69E-46
MS.gene054405	MS.gene67316	0.825392	4.81E-54	-1.69E-46
MS.gene054559	MS.gene67316	0.820665	6.08E-53	-1.69E-46
MS.gene054657	MS.gene67316	0.813936	1.99E-51	-1.69E-46
MS.gene054930	MS.gene67316	0.80191	7.20E-49	-1.69E-46
MS.gene05510	MS.gene67316	0.849062	4.11E-60	-1.69E-46
MS.gene055230	MS.gene67316	0.803263	3.79E-49	-1.69E-46
MS.gene055356	MS.gene67316	0.811695	6.15E-51	-1.69E-46
MS.gene055367	MS.gene67316	0.815013	1.15E-51	-1.69E-46
MS.gene055701	MS.gene67316	0.883783	3.35E-71	-1.69E-46
MS.gene056506	MS.gene67316	0.818837	1.59E-52	-1.69E-46
MS.gene056979	MS.gene67316	0.818018	2.44E-52	-1.69E-46
MS.gene057060	MS.gene67316	0.820036	8.48E-53	-1.69E-46
MS.gene057156	MS.gene67316	-0.816887	4.39E-52	-1.69E-46
MS.gene05761	MS.gene67316	0.808351	3.23E-50	-1.69E-46
MS.gene057639	MS.gene67316	0.835952	1.24E-56	-1.69E-46
MS.gene057693	MS.gene67316	0.802758	4.82E-49	-1.69E-46
MS.gene058146	MS.gene67316	0.832504	9.10E-56	-1.69E-46
MS.gene058548	MS.gene67316	0.825748	3.96E-54	-1.69E-46
MS.gene058595	MS.gene67316	0.801367	9.31E-49	-1.69E-46
MS.gene059609	MS.gene67316	0.822119	2.81E-53	-1.69E-46
MS.gene059710	MS.gene67316	0.805741	1.15E-49	-1.69E-46
MS.gene05979	MS.gene67316	0.815203	1.04E-51	-1.69E-46
MS.gene060011	MS.gene67316	0.811257	7.66E-51	-1.69E-46
MS.gene060310	MS.gene67316	0.804464	2.13E-49	-1.69E-46
MS.gene060803	MS.gene67316	0.803252	3.81E-49	-1.69E-46
MS.gene061040	MS.gene67316	-0.819953	8.86E-53	-1.69E-46
MS.gene061114	MS.gene67316	-0.831192	1.92E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67316.t1	MTR_2g435780	83.099	71	8	1	1	67	1	71	2.55e-35	114
MS.gene67316.t1	MTR_2g435740	83.562	73	7	3	1	69	1	72	7.30e-34	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAAGGAAGATTGGCTTAGA+AGG	0.290742	2.3:+55181053	MS.gene67316:CDS
TGCATGCTGCTCCTTCTTGT+TGG	0.293203	2.3:-55181104	None:intergenic
AGCATGCAAGACCTTGCTTC+TGG	0.370001	2.3:+55181119	MS.gene67316:CDS
AGGATGGAACTGTTCTTGTT+GGG	0.402264	2.3:+55181150	MS.gene67316:CDS
AAGGATGGAACTGTTCTTGT+TGG	0.408271	2.3:+55181149	MS.gene67316:CDS
TCATCAGACCTAAGGAAGAT+TGG	0.412607	2.3:+55181044	MS.gene67316:CDS
GCTTTAATAGACAAGTTCTA+TGG	0.431998	2.3:+55181080	MS.gene67316:CDS
GCATGCAAGACCTTGCTTCT+GGG	0.437774	2.3:+55181120	MS.gene67316:CDS
ATCAAAGTCTTCTGTAGCTA+AGG	0.443745	2.3:+55181328	MS.gene67316:CDS
CTTCTAAGCCAATCTTCCTT+AGG	0.445559	2.3:-55181052	None:intergenic
TCAAAGTCTTCTGTAGCTAA+GGG	0.531279	2.3:+55181329	MS.gene67316:CDS
TAAGGAAGATTGGCTTAGAA+GGG	0.540665	2.3:+55181054	MS.gene67316:CDS
GCTTCTGGGATTAGCAAGGA+TGG	0.553628	2.3:+55181134	MS.gene67316:CDS
CACCTCCTTCATCAGACCTA+AGG	0.563117	2.3:+55181036	MS.gene67316:CDS
AGTTCTATGGTCCAACAAGA+AGG	0.572566	2.3:+55181093	MS.gene67316:CDS
GGATGGAACTGTTCTTGTTG+GGG	0.579567	2.3:+55181151	MS.gene67316:CDS
ATCTTCCTTAGGTCTGATGA+AGG	0.601958	2.3:-55181041	None:intergenic
CCTTGCTTCTGGGATTAGCA+AGG	0.605996	2.3:+55181130	MS.gene67316:CDS
CCTTGCTAATCCCAGAAGCA+AGG	0.646637	2.3:-55181130	None:intergenic
TTCCTTAGGTCTGATGAAGG+AGG	0.672818	2.3:-55181038	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAGTGCTTAAAATTTAAA+AGG	-	chr2.3:55181221-55181240	None:intergenic	15.0%
!!	AATTTGCCTTAAATTTAAAG+TGG	+	chr2.3:55181238-55181257	MS.gene67316:intron	20.0%
!!!	ATTTGCCTTAAATTTAAAGT+GGG	+	chr2.3:55181239-55181258	MS.gene67316:intron	20.0%
	AAAATTACCCCAAAATCGTT+GGG	+	chr2.3:55181190-55181209	MS.gene67316:intron	30.0%
	ATATGTAGAAAAAAGCTCGA+AGG	+	chr2.3:55181288-55181307	MS.gene67316:intron	30.0%
	GCTTTAATAGACAAGTTCTA+TGG	+	chr2.3:55181080-55181099	MS.gene67316:CDS	30.0%
	TGAGACCCACTTTAAATTTA+AGG	-	chr2.3:55181247-55181266	None:intergenic	30.0%
	AAATTACCCCAAAATCGTTG+GGG	+	chr2.3:55181191-55181210	MS.gene67316:intron	35.0%
	ATCAAAGTCTTCTGTAGCTA+AGG	+	chr2.3:55181328-55181347	MS.gene67316:CDS	35.0%
	GAAAATTACCCCAAAATCGT+TGG	+	chr2.3:55181189-55181208	MS.gene67316:intron	35.0%
	TCAAAGTCTTCTGTAGCTAA+GGG	+	chr2.3:55181329-55181348	MS.gene67316:CDS	35.0%
!!	TAAGGAAGATTGGCTTAGAA+GGG	+	chr2.3:55181054-55181073	MS.gene67316:CDS	35.0%
!!	TTTGGGGTAATTTTCCCTTA+GGG	-	chr2.3:55181184-55181203	None:intergenic	35.0%
!!!	TTTTGGGGTAATTTTCCCTT+AGG	-	chr2.3:55181185-55181204	None:intergenic	35.0%
	AAGGATGGAACTGTTCTTGT+TGG	+	chr2.3:55181149-55181168	MS.gene67316:CDS	40.0%
	AGGATGGAACTGTTCTTGTT+GGG	+	chr2.3:55181150-55181169	MS.gene67316:CDS	40.0%
	AGTTCTATGGTCCAACAAGA+AGG	+	chr2.3:55181093-55181112	MS.gene67316:CDS	40.0%
	ATCTTCCTTAGGTCTGATGA+AGG	-	chr2.3:55181044-55181063	None:intergenic	40.0%
	CTTCTAAGCCAATCTTCCTT+AGG	-	chr2.3:55181055-55181074	None:intergenic	40.0%
	TCATCAGACCTAAGGAAGAT+TGG	+	chr2.3:55181044-55181063	MS.gene67316:CDS	40.0%
	TGGGTCTCAGTTTCATGTTA+TGG	+	chr2.3:55181258-55181277	MS.gene67316:intron	40.0%
!!	CTAAGGAAGATTGGCTTAGA+AGG	+	chr2.3:55181053-55181072	MS.gene67316:CDS	40.0%
	GGATGGAACTGTTCTTGTTG+GGG	+	chr2.3:55181151-55181170	MS.gene67316:CDS	45.0%
	GTTGGGGTAAGTTACCCTAA+GGG	+	chr2.3:55181167-55181186	MS.gene67316:intron	45.0%
	TGTTGGGGTAAGTTACCCTA+AGG	+	chr2.3:55181166-55181185	MS.gene67316:intron	45.0%
	TTCCTTAGGTCTGATGAAGG+AGG	-	chr2.3:55181041-55181060	None:intergenic	45.0%
!	AAGGTCTACCCCAACGATTT+TGG	-	chr2.3:55181202-55181221	None:intergenic	45.0%
!	AGGTCTACCCCAACGATTTT+GGG	-	chr2.3:55181201-55181220	None:intergenic	45.0%
	AGCATGCAAGACCTTGCTTC+TGG	+	chr2.3:55181119-55181138	MS.gene67316:CDS	50.0%
	CACCTCCTTCATCAGACCTA+AGG	+	chr2.3:55181036-55181055	MS.gene67316:CDS	50.0%
	CCTTGCTAATCCCAGAAGCA+AGG	-	chr2.3:55181133-55181152	None:intergenic	50.0%
	GCATGCAAGACCTTGCTTCT+GGG	+	chr2.3:55181120-55181139	MS.gene67316:CDS	50.0%
	TGCATGCTGCTCCTTCTTGT+TGG	-	chr2.3:55181107-55181126	None:intergenic	50.0%
!	GGTCTACCCCAACGATTTTG+GGG	-	chr2.3:55181200-55181219	None:intergenic	50.0%
!!	CCTTGCTTCTGGGATTAGCA+AGG	+	chr2.3:55181130-55181149	MS.gene67316:CDS	50.0%
!!	GCTTCTGGGATTAGCAAGGA+TGG	+	chr2.3:55181134-55181153	MS.gene67316:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	55181032	55181364	55181032	ID=MS.gene67316
chr2.3	mRNA	55181032	55181364	55181032	ID=MS.gene67316.t1;Parent=MS.gene67316
chr2.3	exon	55181032	55181172	55181032	ID=MS.gene67316.t1.exon1;Parent=MS.gene67316.t1
chr2.3	CDS	55181032	55181172	55181032	ID=cds.MS.gene67316.t1;Parent=MS.gene67316.t1
chr2.3	exon	55181296	55181364	55181296	ID=MS.gene67316.t1.exon2;Parent=MS.gene67316.t1
chr2.3	CDS	55181296	55181364	55181296	ID=cds.MS.gene67316.t1;Parent=MS.gene67316.t1

Gene Sequence	>MS.gene67316 ATGGCACCTCCTTCATCAGACCTAAGGAAGATTGGCTTAGAAGGGTTTGCTTTAATAGACAAGTTCTATGGTCCAACAAGAAGGAGCAGCATGCAAGACCTTGCTTCTGGGATTAGCAAGGATGGAACTGTTCTTGTTGGGGTAAGTTACCCTAAGGGAAAATTACCCCAAAATCGTTGGGGTAGACCTTTTAAATTTTAAGCACTAATTTGCCTTAAATTTAAAGTGGGTCTCAGTTTCATGTTATGGTATATATATATGTAGAAAAAAGCTCGAAGGCATGTGATCATATTTAAATCAAAGTCTTCTGTAGCTAAGGGAGAGTATATGTAG
Protein sequence	>MS.gene67316.t1 MAPPSSDLRKIGLEGFALIDKFYGPTRRSSMQDLASGISKDGTVLVGKKARRHVIIFKSKSSVAKGEYM