AlfalfaGEDB | MS.gene75625

M. sativa cultivar XinJiangDaYe / MS.gene75625

KEGG: K02915
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01199.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75625.t1	XP_003621181.1	100	101	0	0	1	101	1	101	8.80E-49	202.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75625.t1	P40590	99.0	101	1	0	1	101	1	101	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75625.t1	Q2HVS3	100.0	101	0	0	1	101	1	101	6.4e-49	202.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene75625	0.803716	3.05E-49	-1.69E-46
MS.gene049825	MS.gene75625	0.804614	1.99E-49	-1.69E-46
MS.gene050277	MS.gene75625	0.84239	2.66E-58	-1.69E-46
MS.gene050290	MS.gene75625	0.817194	3.74E-52	-1.69E-46
MS.gene050303	MS.gene75625	0.834882	2.32E-56	-1.69E-46
MS.gene050838	MS.gene75625	0.84698	1.54E-59	-1.69E-46
MS.gene050916	MS.gene75625	0.834041	3.77E-56	-1.69E-46
MS.gene051003	MS.gene75625	0.805764	1.14E-49	-1.69E-46
MS.gene051063	MS.gene75625	0.800244	1.58E-48	-1.69E-46
MS.gene051328	MS.gene75625	0.838876	2.22E-57	-1.69E-46
MS.gene051330	MS.gene75625	0.877547	5.76E-69	-1.69E-46
MS.gene05134	MS.gene75625	0.812954	3.27E-51	-1.69E-46
MS.gene051676	MS.gene75625	0.802611	5.17E-49	-1.69E-46
MS.gene051700	MS.gene75625	0.858172	9.89E-63	-1.69E-46
MS.gene051746	MS.gene75625	0.865926	4.14E-65	-1.69E-46
MS.gene051748	MS.gene75625	0.849743	2.66E-60	-1.69E-46
MS.gene051791	MS.gene75625	0.81441	1.56E-51	-1.69E-46
MS.gene051850	MS.gene75625	0.815472	9.09E-52	-1.69E-46
MS.gene05188	MS.gene75625	0.866852	2.10E-65	-1.69E-46
MS.gene051903	MS.gene75625	0.871758	5.35E-67	-1.69E-46
MS.gene051975	MS.gene75625	0.845541	3.81E-59	-1.69E-46
MS.gene052133	MS.gene75625	0.81978	9.71E-53	-1.69E-46
MS.gene052947	MS.gene75625	0.916241	2.26E-85	-1.69E-46
MS.gene053109	MS.gene75625	0.802454	5.57E-49	-1.69E-46
MS.gene053952	MS.gene75625	0.818642	1.76E-52	-1.69E-46
MS.gene054937	MS.gene75625	0.835021	2.14E-56	-1.69E-46
MS.gene055424	MS.gene75625	0.802438	5.61E-49	-1.69E-46
MS.gene055468	MS.gene75625	0.807757	4.32E-50	-1.69E-46
MS.gene055555	MS.gene75625	0.834584	2.75E-56	-1.69E-46
MS.gene055580	MS.gene75625	-0.8085	3.00E-50	-1.69E-46
MS.gene055603	MS.gene75625	0.804583	2.02E-49	-1.69E-46
MS.gene055808	MS.gene75625	0.845969	2.91E-59	-1.69E-46
MS.gene056043	MS.gene75625	-0.819116	1.38E-52	-1.69E-46
MS.gene056075	MS.gene75625	0.850647	1.48E-60	-1.69E-46
MS.gene056091	MS.gene75625	0.841777	3.87E-58	-1.69E-46
MS.gene056215	MS.gene75625	0.86311	3.15E-64	-1.69E-46
MS.gene056605	MS.gene75625	0.804347	2.26E-49	-1.69E-46
MS.gene056631	MS.gene75625	0.891088	5.47E-74	-1.69E-46
MS.gene05689	MS.gene75625	0.822359	2.47E-53	-1.69E-46
MS.gene056986	MS.gene75625	0.837465	5.12E-57	-1.69E-46
MS.gene057094	MS.gene75625	0.884423	1.94E-71	-1.69E-46
MS.gene057234	MS.gene75625	0.803321	3.69E-49	-1.69E-46
MS.gene057705	MS.gene75625	0.842437	2.59E-58	-1.69E-46
MS.gene058084	MS.gene75625	0.822719	2.04E-53	-1.69E-46
MS.gene058238	MS.gene75625	0.820856	5.50E-53	-1.69E-46
MS.gene058852	MS.gene75625	0.808647	2.79E-50	-1.69E-46
MS.gene058906	MS.gene75625	0.841224	5.41E-58	-1.69E-46
MS.gene059146	MS.gene75625	0.809296	2.03E-50	-1.69E-46
MS.gene059227	MS.gene75625	0.833752	4.45E-56	-1.69E-46
MS.gene05954	MS.gene75625	0.803582	3.25E-49	-1.69E-46
MS.gene06001	MS.gene75625	0.909944	3.25E-82	-1.69E-46
MS.gene06011	MS.gene75625	0.859292	4.57E-63	-1.69E-46
MS.gene060429	MS.gene75625	0.902093	1.38E-78	-1.69E-46
MS.gene060586	MS.gene75625	0.834326	3.20E-56	-1.69E-46
MS.gene060763	MS.gene75625	0.82978	4.25E-55	-1.69E-46
MS.gene060830	MS.gene75625	0.820033	8.49E-53	-1.69E-46
MS.gene060917	MS.gene75625	0.840351	9.16E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75625.t1	MTR_5g040570	100.000	101	0	0	1	101	1	101	4.74e-70	205
MS.gene75625.t1	MTR_7g010150	100.000	101	0	0	1	101	1	101	5.00e-70	204
MS.gene75625.t1	MTR_8g467460	100.000	101	0	0	1	101	1	101	8.20e-70	204
MS.gene75625.t1	MTR_7g011050	68.317	101	16	1	1	101	1	85	8.98e-42	133

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75625.t1	AT1G26880	94.059	101	6	1	101	1	101	5.46e-66	194
MS.gene75625.t1	AT1G69620	94.059	101	6	1	101	1	101	1.10e-65	194
MS.gene75625.t1	AT3G28900	90.099	101	10	1	101	1	101	5.74e-63	187
MS.gene75625.t1	AT1G26880	91.304	92	8	1	92	1	92	1.76e-57	172

Find 33 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAACCGCCGCACTGTTAAC+AGG	0.305052	3.3:+71087698	MS.gene75625:CDS
TGGATCCTCTTTCCAGTAAC+AGG	0.314412	3.3:-71087625	None:intergenic
GGACCTAAGTGTCCTGTTAC+TGG	0.331250	3.3:+71087613	MS.gene75625:CDS
CGCACTGTTAACAGGGCTTA+TGG	0.356563	3.3:+71087706	MS.gene75625:CDS
AACACGATGCTGGTTGGATT+TGG	0.389836	3.3:-71087536	None:intergenic
TCTTTCCCTAACAGCTCCTC+CGG	0.456903	3.3:-71087740	None:intergenic
TTAACAGTGCGGCGGTTCCT+AGG	0.468395	3.3:-71087694	None:intergenic
TTTATCCGGAGGAGCTGTTA+GGG	0.501294	3.3:+71087735	MS.gene75625:CDS
TTTCCAGTAACAGGACACTT+AGG	0.509665	3.3:-71087616	None:intergenic
GTCTCAAGTGTGGAATCCCT+TGG	0.518456	3.3:-71087645	None:intergenic
ACGATGCTGGTTGGATTTGG+TGG	0.532546	3.3:-71087533	None:intergenic
CATCGTGTTGTTAAGACACC+TGG	0.534929	3.3:+71087550	MS.gene75625:CDS
TAAGACACCTGGTGGAAAGT+TGG	0.535804	3.3:+71087561	MS.gene75625:CDS
AGTGTCCTGTTACTGGAAAG+AGG	0.543803	3.3:+71087620	MS.gene75625:CDS
CTTAACAACACGATGCTGGT+TGG	0.545517	3.3:-71087542	None:intergenic
GTTACTGGAAAGAGGATCCA+AGG	0.571456	3.3:+71087628	MS.gene75625:CDS
ACAACTAAGAAGAGAGCTAG+TGG	0.577057	3.3:+71087592	MS.gene75625:CDS
GTGTCTTAACAACACGATGC+TGG	0.578379	3.3:-71087546	None:intergenic
TGGCATAGCTGTGTCTCCTA+CGG	0.580308	3.3:-71087513	None:intergenic
GCTCTTCAACAAGGAAAGCA+CGG	0.587996	3.3:-71087768	None:intergenic
GAACCGCCGCACTGTTAACA+GGG	0.611783	3.3:+71087699	MS.gene75625:CDS
CTAGACCTCTTGTATTCAGT+AGG	0.617053	3.3:-71087667	None:intergenic
TGAGACCTACTGAATACAAG+AGG	0.631121	3.3:+71087662	MS.gene75625:CDS
CGTGTTGTTAAGACACCTGG+TGG	0.634441	3.3:+71087553	MS.gene75625:CDS
ACAAGAGGTCTAGATTGCCT+AGG	0.636078	3.3:+71087677	MS.gene75625:CDS
TTACTGGAAAGAGGATCCAA+GGG	0.655184	3.3:+71087629	MS.gene75625:CDS
TGATAAACCAACTTTCCACC+AGG	0.658331	3.3:-71087568	None:intergenic
TATTCAGTAGGTCTCAAGTG+TGG	0.667813	3.3:-71087655	None:intergenic
AAGCCCTGTTAACAGTGCGG+CGG	0.691394	3.3:-71087702	None:intergenic
ACTGTTAACAGGGCTTATGG+AGG	0.693206	3.3:+71087709	MS.gene75625:CDS
TTCAGAGACTCACATACCGT+AGG	0.721913	3.3:+71087497	MS.gene75625:CDS
CATAAGCCCTGTTAACAGTG+CGG	0.730145	3.3:-71087705	None:intergenic
GTATGTGAGTCTCTGAACCA+TGG	0.739024	3.3:-71087491	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATTTTTTTGCTCTTCAACA+AGG	-	chr3.3:71087780-71087799	None:intergenic	25.0%
	ACAACTAAGAAGAGAGCTAG+TGG	+	chr3.3:71087592-71087611	MS.gene75625:CDS	40.0%
	CTAGACCTCTTGTATTCAGT+AGG	-	chr3.3:71087670-71087689	None:intergenic	40.0%
	TATTCAGTAGGTCTCAAGTG+TGG	-	chr3.3:71087658-71087677	None:intergenic	40.0%
	TGAGACCTACTGAATACAAG+AGG	+	chr3.3:71087662-71087681	MS.gene75625:CDS	40.0%
	TGATAAACCAACTTTCCACC+AGG	-	chr3.3:71087571-71087590	None:intergenic	40.0%
	TTACTGGAAAGAGGATCCAA+GGG	+	chr3.3:71087629-71087648	MS.gene75625:CDS	40.0%
	TTTCCAGTAACAGGACACTT+AGG	-	chr3.3:71087619-71087638	None:intergenic	40.0%
!!!	GCTTATGGAGGTGTTTTATC+CGG	+	chr3.3:71087721-71087740	MS.gene75625:CDS	40.0%
	ACAAGAGGTCTAGATTGCCT+AGG	+	chr3.3:71087677-71087696	MS.gene75625:CDS	45.0%
	ACTGTTAACAGGGCTTATGG+AGG	+	chr3.3:71087709-71087728	MS.gene75625:CDS	45.0%
	AGTGTCCTGTTACTGGAAAG+AGG	+	chr3.3:71087620-71087639	MS.gene75625:CDS	45.0%
	CATAAGCCCTGTTAACAGTG+CGG	-	chr3.3:71087708-71087727	None:intergenic	45.0%
	CTTAACAACACGATGCTGGT+TGG	-	chr3.3:71087545-71087564	None:intergenic	45.0%
	GCTCTTCAACAAGGAAAGCA+CGG	-	chr3.3:71087771-71087790	None:intergenic	45.0%
	GTGTCTTAACAACACGATGC+TGG	-	chr3.3:71087549-71087568	None:intergenic	45.0%
	GTTACTGGAAAGAGGATCCA+AGG	+	chr3.3:71087628-71087647	MS.gene75625:CDS	45.0%
	TAAGACACCTGGTGGAAAGT+TGG	+	chr3.3:71087561-71087580	MS.gene75625:CDS	45.0%
	TGGATCCTCTTTCCAGTAAC+AGG	-	chr3.3:71087628-71087647	None:intergenic	45.0%
	TTCAGAGACTCACATACCGT+AGG	+	chr3.3:71087497-71087516	MS.gene75625:CDS	45.0%
	TTTATCCGGAGGAGCTGTTA+GGG	+	chr3.3:71087735-71087754	MS.gene75625:CDS	45.0%
!	AACACGATGCTGGTTGGATT+TGG	-	chr3.3:71087539-71087558	None:intergenic	45.0%
!	CATCGTGTTGTTAAGACACC+TGG	+	chr3.3:71087550-71087569	MS.gene75625:CDS	45.0%
!	TTTTATCCGGAGGAGCTGTT+AGG	+	chr3.3:71087734-71087753	MS.gene75625:CDS	45.0%
!!!	TATGGAGGTGTTTTATCCGG+AGG	+	chr3.3:71087724-71087743	MS.gene75625:CDS	45.0%
	CGCACTGTTAACAGGGCTTA+TGG	+	chr3.3:71087706-71087725	MS.gene75625:CDS	50.0%
	GGACCTAAGTGTCCTGTTAC+TGG	+	chr3.3:71087613-71087632	MS.gene75625:CDS	50.0%
	GTCTCAAGTGTGGAATCCCT+TGG	-	chr3.3:71087648-71087667	None:intergenic	50.0%
	TCTTTCCCTAACAGCTCCTC+CGG	-	chr3.3:71087743-71087762	None:intergenic	50.0%
	TGGCATAGCTGTGTCTCCTA+CGG	-	chr3.3:71087516-71087535	None:intergenic	50.0%
!	ACGATGCTGGTTGGATTTGG+TGG	-	chr3.3:71087536-71087555	None:intergenic	50.0%
!	CGTGTTGTTAAGACACCTGG+TGG	+	chr3.3:71087553-71087572	MS.gene75625:CDS	50.0%
	AAGCCCTGTTAACAGTGCGG+CGG	-	chr3.3:71087705-71087724	None:intergenic	55.0%
	GAACCGCCGCACTGTTAACA+GGG	+	chr3.3:71087699-71087718	MS.gene75625:CDS	55.0%
	TTAACAGTGCGGCGGTTCCT+AGG	-	chr3.3:71087697-71087716	None:intergenic	55.0%
	GGAACCGCCGCACTGTTAAC+AGG	+	chr3.3:71087698-71087717	MS.gene75625:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	71087493	71087804	71087493	ID=MS.gene75625
chr3.3	mRNA	71087493	71087804	71087493	ID=MS.gene75625.t1;Parent=MS.gene75625
chr3.3	exon	71087493	71087804	71087493	ID=MS.gene75625.t1.exon1;Parent=MS.gene75625.t1
chr3.3	CDS	71087493	71087804	71087493	ID=cds.MS.gene75625.t1;Parent=MS.gene75625.t1

Gene Sequence	>MS.gene75625 ATGGTTCAGAGACTCACATACCGTAGGAGACACAGCTATGCCACCAAATCCAACCAGCATCGTGTTGTTAAGACACCTGGTGGAAAGTTGGTTTATCAGACAACTAAGAAGAGAGCTAGTGGACCTAAGTGTCCTGTTACTGGAAAGAGGATCCAAGGGATTCCACACTTGAGACCTACTGAATACAAGAGGTCTAGATTGCCTAGGAACCGCCGCACTGTTAACAGGGCTTATGGAGGTGTTTTATCCGGAGGAGCTGTTAGGGAAAGAATTATCCGTGCTTTCCTTGTTGAAGAGCAAAAAAATAGTTAA
Protein sequence	>MS.gene75625.t1 MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEEQKNS