AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76305


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76305.t1 MTR_1g110760 95.092 163 5 1 1 163 1 160 2.47e-106 300
MS.gene76305.t1 MTR_6g018430 45.963 161 67 6 4 163 27 168 2.11e-34 119
MS.gene76305.t1 MTR_8g104210 45.399 163 68 8 7 163 16 163 5.30e-28 102
MS.gene76305.t1 MTR_8g104210 45.399 163 68 8 7 163 25 172 7.65e-28 102
MS.gene76305.t1 MTR_6g084470 45.732 164 67 7 5 163 21 167 7.60e-27 99.8
MS.gene76305.t1 MTR_1g051170 38.931 131 60 4 26 149 31 148 4.91e-13 63.5
MS.gene76305.t1 MTR_4g087400 38.732 142 58 5 9 149 22 135 1.79e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76305.t1 AT1G68490 59.756 164 53 6 4 163 29 183 4.78e-49 156
MS.gene76305.t1 AT1G13390 51.852 162 66 5 4 163 25 176 8.24e-41 135
MS.gene76305.t1 AT1G13390 51.852 162 66 5 4 163 25 176 8.24e-41 135
MS.gene76305.t1 AT5G16110 50.909 165 67 7 4 163 89 244 5.19e-35 122
MS.gene76305.t1 AT3G02555 46.296 162 56 5 5 163 29 162 5.82e-27 99.8
MS.gene76305.t1 AT3G02555 46.296 162 56 5 5 163 29 162 5.82e-27 99.8

Find 65 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCGGATTGTTACCAAAAT+TGG 0.118190 1.1:-78218941 None:intergenic
GGATGTGTTCGGGCCAATTT+TGG 0.234336 1.1:+78218928 MS.gene76305:CDS
TGCCCTAAGCCCCGCCGTTT+CGG 0.287589 1.1:+78218210 MS.gene76305:CDS
AGTCCGAAACGGCGGGGCTT+AGG 0.324326 1.1:-78218213 None:intergenic
AGTAGCTAACCCACTAATTC+AGG 0.341828 1.1:+78218795 MS.gene76305:CDS
TAATTCAGGATGCTCGATTC+GGG 0.363565 1.1:+78218809 MS.gene76305:CDS
CCTCCCTTCCTTGCAGAAGA+TGG 0.393816 1.1:-78218907 None:intergenic
CTAATTCAGGATGCTCGATT+CGG 0.401608 1.1:+78218808 MS.gene76305:CDS
AGAAGGTGGCAGACCGGATG+GGG 0.404001 1.1:-78218881 None:intergenic
GAGCAGGGATGCTGCAATTA+CGG 0.410027 1.1:-78219002 None:intergenic
GTCCGAAACGGCGGGGCTTA+GGG 0.414885 1.1:-78218212 None:intergenic
CGCCCCTCTCACCGTCGTCA+TGG 0.419708 1.1:+78218845 MS.gene76305:CDS
CAAGGAAGGGAGGATGTGTT+CGG 0.421741 1.1:+78218917 MS.gene76305:CDS
GATCCTTCCGGTGGCATCTC+AGG 0.425952 1.1:+78218268 MS.gene76305:CDS
CTCCCTTCCTTGCAGAAGAT+GGG 0.432493 1.1:-78218906 None:intergenic
AAGGATTCGATTGCCTTGAC+AGG 0.436802 1.1:+78218974 MS.gene76305:CDS
TAATTGCAGCATCCCTGCTC+TGG 0.448656 1.1:+78219005 MS.gene76305:CDS
GCCCCTCTCACCGTCGTCAT+GGG 0.457309 1.1:+78218846 MS.gene76305:CDS
GGAGAAGGTGGCAGACCGGA+TGG 0.458776 1.1:-78218883 None:intergenic
CTCATCTGCGTCAAATCCCT+TGG 0.463633 1.1:+78218417 MS.gene76305:CDS
GGTGGCAGACCGGATGGGGC+AGG 0.466438 1.1:-78218877 None:intergenic
CTGTTGTTCTAAGCCAGAGC+AGG 0.481278 1.1:-78219018 None:intergenic
GAGAAGGTGGCAGACCGGAT+GGG 0.484866 1.1:-78218882 None:intergenic
GTTGAGGAGTCCGAAACGGC+GGG 0.491911 1.1:-78218220 None:intergenic
ACTACCCGAGCAGATCCTTC+CGG 0.494170 1.1:+78218256 MS.gene76305:CDS
GTGGTGGTTCCTGCCCCATC+CGG 0.495940 1.1:+78218868 MS.gene76305:CDS
TTGAATCACACGGTTCAACT+TGG 0.496164 1.1:-78218393 None:intergenic
CTCACCGTCGTCATGGGTGG+TGG 0.513742 1.1:+78218852 MS.gene76305:CDS
TCTGCTCGGGTAGTCGTTGA+CGG 0.526759 1.1:-78218247 None:intergenic
CCACCCATGACGACGGTGAG+AGG 0.533000 1.1:-78218849 None:intergenic
AATCCGGCGGTGAGAGTCGA+AGG 0.545936 1.1:+78218955 MS.gene76305:CDS
TCCCTTCCTTGCAGAAGATG+GGG 0.546203 1.1:-78218905 None:intergenic
AATCGAGCATCCTGAATTAG+TGG 0.550154 1.1:-78218805 None:intergenic
AGTCGTTGACGGCGAAGTTG+AGG 0.554697 1.1:-78218236 None:intergenic
CACCCATGACGACGGTGAGA+GGG 0.562391 1.1:-78218848 None:intergenic
AAGGAAGGGAGGATGTGTTC+GGG 0.568345 1.1:+78218918 MS.gene76305:CDS
GCCACCGGAAGGATCTGCTC+GGG 0.575084 1.1:-78218260 None:intergenic
CGAAGTTGAGGAGTCCGAAA+CGG 0.580614 1.1:-78218224 None:intergenic
TTGGTGAGAATTGTGTCCAA+GGG 0.586012 1.1:-78218433 None:intergenic
CCTCTCACCGTCGTCATGGG+TGG 0.588491 1.1:+78218849 MS.gene76305:CDS
CTTGGTGAGAATTGTGTCCA+AGG 0.590478 1.1:-78218434 None:intergenic
GCAGGATGATGATTGTGATA+CGG 0.592153 1.1:+78218708 MS.gene76305:intron
AGTGGGTTAGCTACTCTGCT+CGG 0.596257 1.1:-78218787 None:intergenic
TGCCACCGGAAGGATCTGCT+CGG 0.596625 1.1:-78218261 None:intergenic
AGGATTCGATTGCCTTGACA+GGG 0.597575 1.1:+78218975 MS.gene76305:CDS
CCATCTTCTGCAAGGAAGGG+AGG 0.605126 1.1:+78218907 MS.gene76305:CDS
CCCTTCCTTGCAGAAGATGG+GGG 0.610451 1.1:-78218904 None:intergenic
ACCCATGACGACGGTGAGAG+GGG 0.613698 1.1:-78218847 None:intergenic
AATCCTTCGACTCTCACCGC+CGG 0.616188 1.1:-78218958 None:intergenic
AGTTGAGGAGTCCGAAACGG+CGG 0.629025 1.1:-78218221 None:intergenic
AATTACGGCTGTCCCTGTCA+AGG 0.629306 1.1:-78218987 None:intergenic
ATCGAGCATCCTGAATTAGT+GGG 0.630666 1.1:-78218804 None:intergenic
GGCGGCGACGAGGCTACTGG+AGG 0.632101 1.1:-78218742 None:intergenic
AAATTCACCTGAGATGCCAC+CGG 0.634810 1.1:-78218275 None:intergenic
TCACCTGAGATGCCACCGGA+AGG 0.642394 1.1:-78218271 None:intergenic
TTTCGACGAAGGAAGATGAA+CGG 0.643470 1.1:+78218171 None:intergenic
GGAACCACCACCCATGACGA+CGG 0.659927 1.1:-78218856 None:intergenic
GCAGATGAGTTTGAATCACA+CGG 0.662013 1.1:-78218403 None:intergenic
TGTTGTTCTAAGCCAGAGCA+GGG 0.670625 1.1:-78219017 None:intergenic
ACCCGAGCAGATCCTTCCGG+TGG 0.672658 1.1:+78218259 MS.gene76305:CDS
AATTCAGGATGCTCGATTCG+GGG 0.678593 1.1:+78218810 MS.gene76305:CDS
GCGGGGCTTAGGGCAAACGA+CGG 0.692402 1.1:-78218202 None:intergenic
CTTGGACACAATTCTCACCA+AGG 0.706031 1.1:+78218435 MS.gene76305:CDS
TTGAGGAGTCCGAAACGGCG+GGG 0.712058 1.1:-78218219 None:intergenic
GGGTTAGCTACTCTGCTCGG+CGG 0.725325 1.1:-78218784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCATATAAAATAATAG+AGG - chr1.1:78218470-78218489 None:intergenic 10.0%
!!! CTATTATTTTATATGAATTT+AGG + chr1.1:78218470-78218489 MS.gene76305:intron 10.0%
!!! AATTGATCTATTTTTAACAT+TGG + chr1.1:78218548-78218567 MS.gene76305:intron 15.0%
!!! ATATGAATTTAGGTTAAAAT+TGG + chr1.1:78218480-78218499 MS.gene76305:intron 15.0%
!!! TTTTCTCTTTTAATTTGCTT+TGG + chr1.1:78218320-78218339 MS.gene76305:intron 20.0%
! AATAGAGGAATTATTTACCT+TGG - chr1.1:78218455-78218474 None:intergenic 25.0%
ACACAAAGCAATTCAACAAA+AGG - chr1.1:78218644-78218663 None:intergenic 30.0%
AATCGAGCATCCTGAATTAG+TGG - chr1.1:78218808-78218827 None:intergenic 40.0%
AGTAGCTAACCCACTAATTC+AGG + chr1.1:78218795-78218814 MS.gene76305:CDS 40.0%
ATCGAGCATCCTGAATTAGT+GGG - chr1.1:78218807-78218826 None:intergenic 40.0%
CTAATTCAGGATGCTCGATT+CGG + chr1.1:78218808-78218827 MS.gene76305:CDS 40.0%
GCAGATGAGTTTGAATCACA+CGG - chr1.1:78218406-78218425 None:intergenic 40.0%
GCAGGATGATGATTGTGATA+CGG + chr1.1:78218708-78218727 MS.gene76305:intron 40.0%
TAATTCAGGATGCTCGATTC+GGG + chr1.1:78218809-78218828 MS.gene76305:CDS 40.0%
TTGAATCACACGGTTCAACT+TGG - chr1.1:78218396-78218415 None:intergenic 40.0%
TTGGTGAGAATTGTGTCCAA+GGG - chr1.1:78218436-78218455 None:intergenic 40.0%
!! CAATTTTGGTAACAATCCGG+CGG + chr1.1:78218942-78218961 MS.gene76305:CDS 40.0%
!! GGCCAATTTTGGTAACAATC+CGG + chr1.1:78218939-78218958 MS.gene76305:CDS 40.0%
!! GTTGTGCTTTTGTGATTTGC+AGG + chr1.1:78218690-78218709 MS.gene76305:intron 40.0%
AAATTCACCTGAGATGCCAC+CGG - chr1.1:78218278-78218297 None:intergenic 45.0%
AAGGATTCGATTGCCTTGAC+AGG + chr1.1:78218974-78218993 MS.gene76305:CDS 45.0%
AGGATTCGATTGCCTTGACA+GGG + chr1.1:78218975-78218994 MS.gene76305:CDS 45.0%
CGCCGGATTGTTACCAAAAT+TGG - chr1.1:78218944-78218963 None:intergenic 45.0%
CTTGGACACAATTCTCACCA+AGG + chr1.1:78218435-78218454 MS.gene76305:CDS 45.0%
CTTGGTGAGAATTGTGTCCA+AGG - chr1.1:78218437-78218456 None:intergenic 45.0%
! AATTCAGGATGCTCGATTCG+GGG + chr1.1:78218810-78218829 MS.gene76305:CDS 45.0%
AAGGAAGGGAGGATGTGTTC+GGG + chr1.1:78218918-78218937 MS.gene76305:CDS 50.0%
AATTACGGCTGTCCCTGTCA+AGG - chr1.1:78218990-78219009 None:intergenic 50.0%
CAAGGAAGGGAGGATGTGTT+CGG + chr1.1:78218917-78218936 MS.gene76305:CDS 50.0%
CGAAGTTGAGGAGTCCGAAA+CGG - chr1.1:78218227-78218246 None:intergenic 50.0%
CGGAGACCCACAGAAAAATG+GGG - chr1.1:78218767-78218786 None:intergenic 50.0%
CTCATCTGCGTCAAATCCCT+TGG + chr1.1:78218417-78218436 MS.gene76305:CDS 50.0%
CTCCCTTCCTTGCAGAAGAT+GGG - chr1.1:78218909-78218928 None:intergenic 50.0%
GAGCAGGGATGCTGCAATTA+CGG - chr1.1:78219005-78219024 None:intergenic 50.0%
GCGGAGACCCACAGAAAAAT+GGG - chr1.1:78218768-78218787 None:intergenic 50.0%
GGAGACCCACAGAAAAATGG+GGG - chr1.1:78218766-78218785 None:intergenic 50.0%
TAATTGCAGCATCCCTGCTC+TGG + chr1.1:78219005-78219024 MS.gene76305:CDS 50.0%
TCCCTTCCTTGCAGAAGATG+GGG - chr1.1:78218908-78218927 None:intergenic 50.0%
! GGATGTGTTCGGGCCAATTT+TGG + chr1.1:78218928-78218947 MS.gene76305:CDS 50.0%
!! AGTGGGTTAGCTACTCTGCT+CGG - chr1.1:78218790-78218809 None:intergenic 50.0%
AATCCTTCGACTCTCACCGC+CGG - chr1.1:78218961-78218980 None:intergenic 55.0%
ACTACCCGAGCAGATCCTTC+CGG + chr1.1:78218256-78218275 MS.gene76305:CDS 55.0%
AGTCGTTGACGGCGAAGTTG+AGG - chr1.1:78218239-78218258 None:intergenic 55.0%
AGTTGAGGAGTCCGAAACGG+CGG - chr1.1:78218224-78218243 None:intergenic 55.0%
CCATCTTCTGCAAGGAAGGG+AGG + chr1.1:78218907-78218926 MS.gene76305:CDS 55.0%
CCCCCATCTTCTGCAAGGAA+GGG + chr1.1:78218904-78218923 MS.gene76305:CDS 55.0%
CCCTTCCTTGCAGAAGATGG+GGG - chr1.1:78218907-78218926 None:intergenic 55.0%
CCTCCCTTCCTTGCAGAAGA+TGG - chr1.1:78218910-78218929 None:intergenic 55.0%
CTTCTCCCCCATCTTCTGCA+AGG + chr1.1:78218899-78218918 MS.gene76305:CDS 55.0%
CTTGCAGAAGATGGGGGAGA+AGG - chr1.1:78218901-78218920 None:intergenic 55.0%
GACCCACAGAAAAATGGGGG+CGG - chr1.1:78218763-78218782 None:intergenic 55.0%
GGCGGAGACCCACAGAAAAA+TGG - chr1.1:78218769-78218788 None:intergenic 55.0%
TCCCCCATCTTCTGCAAGGA+AGG + chr1.1:78218903-78218922 MS.gene76305:CDS 55.0%
TCTGCTCGGGTAGTCGTTGA+CGG - chr1.1:78218250-78218269 None:intergenic 55.0%
AATCCGGCGGTGAGAGTCGA+AGG + chr1.1:78218955-78218974 MS.gene76305:CDS 60.0%
ACCCATGACGACGGTGAGAG+GGG - chr1.1:78218850-78218869 None:intergenic 60.0%
AGAAGGTGGCAGACCGGATG+GGG - chr1.1:78218884-78218903 None:intergenic 60.0%
CACCCATGACGACGGTGAGA+GGG - chr1.1:78218851-78218870 None:intergenic 60.0%
GAGAAGGTGGCAGACCGGAT+GGG - chr1.1:78218885-78218904 None:intergenic 60.0%
GATCCTTCCGGTGGCATCTC+AGG + chr1.1:78218268-78218287 MS.gene76305:CDS 60.0%
GCAGAAGATGGGGGAGAAGG+TGG - chr1.1:78218898-78218917 None:intergenic 60.0%
GGAACCACCACCCATGACGA+CGG - chr1.1:78218859-78218878 None:intergenic 60.0%
GTTGAGGAGTCCGAAACGGC+GGG - chr1.1:78218223-78218242 None:intergenic 60.0%
TCACCTGAGATGCCACCGGA+AGG - chr1.1:78218274-78218293 None:intergenic 60.0%
TGCCACCGGAAGGATCTGCT+CGG - chr1.1:78218264-78218283 None:intergenic 60.0%
TTGAGGAGTCCGAAACGGCG+GGG - chr1.1:78218222-78218241 None:intergenic 60.0%
!! CGCCGCCCCCATTTTTCTGT+GGG + chr1.1:78218758-78218777 MS.gene76305:CDS 60.0%
!! GGGTTAGCTACTCTGCTCGG+CGG - chr1.1:78218787-78218806 None:intergenic 60.0%
!! TCGCCGCCCCCATTTTTCTG+TGG + chr1.1:78218757-78218776 MS.gene76305:CDS 60.0%
ACCCGAGCAGATCCTTCCGG+TGG + chr1.1:78218259-78218278 MS.gene76305:CDS 65.0%
AGTCCGAAACGGCGGGGCTT+AGG - chr1.1:78218216-78218235 None:intergenic 65.0%
CCACCCATGACGACGGTGAG+AGG - chr1.1:78218852-78218871 None:intergenic 65.0%
CCTCTCACCGTCGTCATGGG+TGG + chr1.1:78218849-78218868 MS.gene76305:CDS 65.0%
CTCACCGTCGTCATGGGTGG+TGG + chr1.1:78218852-78218871 MS.gene76305:CDS 65.0%
GAAAAATGGGGGCGGCGACG+AGG - chr1.1:78218755-78218774 None:intergenic 65.0%
GCCACCGGAAGGATCTGCTC+GGG - chr1.1:78218263-78218282 None:intergenic 65.0%
GCCCCTCTCACCGTCGTCAT+GGG + chr1.1:78218846-78218865 MS.gene76305:CDS 65.0%
GCGGGGCTTAGGGCAAACGA+CGG - chr1.1:78218205-78218224 None:intergenic 65.0%
GGAGAAGGTGGCAGACCGGA+TGG - chr1.1:78218886-78218905 None:intergenic 65.0%
GTCCGAAACGGCGGGGCTTA+GGG - chr1.1:78218215-78218234 None:intergenic 65.0%
GTGGTGGTTCCTGCCCCATC+CGG + chr1.1:78218868-78218887 MS.gene76305:CDS 65.0%
TGCCCTAAGCCCCGCCGTTT+CGG + chr1.1:78218210-78218229 MS.gene76305:CDS 65.0%
TGGGGGAGAAGGTGGCAGAC+CGG - chr1.1:78218890-78218909 None:intergenic 65.0%
CGCCCCTCTCACCGTCGTCA+TGG + chr1.1:78218845-78218864 MS.gene76305:CDS 70.0%
GGCGGCGACGAGGCTACTGG+AGG - chr1.1:78218745-78218764 None:intergenic 75.0%
GGTGGCAGACCGGATGGGGC+AGG - chr1.1:78218880-78218899 None:intergenic 75.0%
! GGGGGCGGCGACGAGGCTAC+TGG - chr1.1:78218748-78218767 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 78218186 78219032 78218186 ID=MS.gene76305
chr1.1 mRNA 78218186 78219032 78218186 ID=MS.gene76305.t1;Parent=MS.gene76305
chr1.1 exon 78218186 78218289 78218186 ID=MS.gene76305.t1.exon1;Parent=MS.gene76305.t1
chr1.1 CDS 78218186 78218289 78218186 ID=cds.MS.gene76305.t1;Parent=MS.gene76305.t1
chr1.1 exon 78218390 78218456 78218390 ID=MS.gene76305.t1.exon2;Parent=MS.gene76305.t1
chr1.1 CDS 78218390 78218456 78218390 ID=cds.MS.gene76305.t1;Parent=MS.gene76305.t1
chr1.1 exon 78218712 78219032 78218712 ID=MS.gene76305.t1.exon3;Parent=MS.gene76305.t1
chr1.1 CDS 78218712 78219032 78218712 ID=cds.MS.gene76305.t1;Parent=MS.gene76305.t1
Gene Sequence

>MS.gene76305

ATGAACGGAAGAGACTCCGTCGTTTGCCCTAAGCCCCGCCGTTTCGGACTCCTCAACTTCGCCGTCAACGACTACCCGAGCAGATCCTTCCGGTGGCATCTCAGGTGAATTTTAATTTCTCTCTGTTTTTCTTCTTTTCTCTTTTAATTTGCTTTGGTTCTATTTTATTAAACTTTTGCATTTTTCGTTTTTCTTCTTCTGCAGTTGCCAAGTTGAACCGTGTGATTCAAACTCATCTGCGTCAAATCCCTTGGACACAATTCTCACCAAGGTAAATAATTCCTCTATTATTTTATATGAATTTAGGTTAAAATTGGAATAATTTATCTATTATTTTATATGAATTTAGGTTAAATTTTAATAATTGATCTATTTTTAACATTGGTTAATGTTAACTTGTTACTTGATAAAATTTGAATAGTTGATTGATTACAGTTAAAGCTTGAATTATTGTGCCTTTTGTTGAATTGCTTTGTGTTATGCTTTTTGATTGAATTGTTCTGTGTTGTGCTTTTGTGATTTGCAGGATGATGATTGTGATACGGAACAATTAAGTCCTCCAGTAGCCTCGTCGCCGCCCCCATTTTTCTGTGGGTCTCCGCCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTCGATTCGGGGATGAGAATTTCTCGCCCCTCTCACCGTCGTCATGGGTGGTGGTTCCTGCCCCATCCGGTCTGCCACCTTCTCCCCCATCTTCTGCAAGGAAGGGAGGATGTGTTCGGGCCAATTTTGGTAACAATCCGGCGGTGAGAGTCGAAGGATTCGATTGCCTTGACAGGGACAGCCGTAATTGCAGCATCCCTGCTCTGGCTTAG

Protein sequence

>MS.gene76305.t1

MNGRDSVVCPKPRRFGLLNFAVNDYPSRSFRWHLSCQVEPCDSNSSASNPLDTILTKDDDCDTEQLSPPVASSPPPFFCGSPPSRVANPLIQDARFGDENFSPLSPSSWVVVPAPSGLPPSPPSSARKGGCVRANFGNNPAVRVEGFDCLDRDSRNCSIPALA