Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84704.t1 | XP_003601922.1 | 94.2 | 154 | 9 | 0 | 1 | 154 | 1 | 154 | 1.10E-76 | 295.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84704.t1 | G7J380 | 94.2 | 154 | 9 | 0 | 1 | 154 | 1 | 154 | 8.3e-77 | 295.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene84704 | 0.828861 | 7.10E-55 | -1.69E-46 |
| MS.gene049259 | MS.gene84704 | 0.806873 | 6.66E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene84704 | 0.859021 | 5.52E-63 | -1.69E-46 |
| MS.gene05015 | MS.gene84704 | 0.80025 | 1.57E-48 | -1.69E-46 |
| MS.gene050363 | MS.gene84704 | 0.831603 | 1.52E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene84704 | 0.847828 | 9.03E-60 | -1.69E-46 |
| MS.gene050496 | MS.gene84704 | -0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene84704 | -0.802088 | 6.62E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene84704 | 0.892199 | 1.98E-74 | -1.69E-46 |
| MS.gene051044 | MS.gene84704 | 0.825658 | 4.16E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene84704 | 0.850344 | 1.80E-60 | -1.69E-46 |
| MS.gene051364 | MS.gene84704 | 0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene051946 | MS.gene84704 | 0.828203 | 1.02E-54 | -1.69E-46 |
| MS.gene052053 | MS.gene84704 | 0.801939 | 7.10E-49 | -1.69E-46 |
| MS.gene052089 | MS.gene84704 | 0.847796 | 9.22E-60 | -1.69E-46 |
| MS.gene052091 | MS.gene84704 | 0.808436 | 3.10E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene84704 | -0.807494 | 4.92E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene84704 | -0.810883 | 9.24E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene84704 | 0.815837 | 7.53E-52 | -1.69E-46 |
| MS.gene053549 | MS.gene84704 | 0.820482 | 6.70E-53 | -1.69E-46 |
| MS.gene053561 | MS.gene84704 | 0.808628 | 2.82E-50 | -1.69E-46 |
| MS.gene053826 | MS.gene84704 | 0.84697 | 1.55E-59 | -1.69E-46 |
| MS.gene054056 | MS.gene84704 | 0.837728 | 4.38E-57 | -1.69E-46 |
| MS.gene054959 | MS.gene84704 | 0.812541 | 4.03E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene84704 | 0.824379 | 8.33E-54 | -1.69E-46 |
| MS.gene055549 | MS.gene84704 | 0.876397 | 1.44E-68 | -1.69E-46 |
| MS.gene055550 | MS.gene84704 | 0.868848 | 4.81E-66 | -1.69E-46 |
| MS.gene055551 | MS.gene84704 | 0.840617 | 7.80E-58 | -1.69E-46 |
| MS.gene05589 | MS.gene84704 | -0.811047 | 8.51E-51 | -1.69E-46 |
| MS.gene055942 | MS.gene84704 | 0.82429 | 8.75E-54 | -1.69E-46 |
| MS.gene056310 | MS.gene84704 | 0.826313 | 2.91E-54 | -1.69E-46 |
| MS.gene056311 | MS.gene84704 | 0.863673 | 2.10E-64 | -1.69E-46 |
| MS.gene056365 | MS.gene84704 | 0.810558 | 1.09E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene84704 | -0.822266 | 2.60E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene84704 | 0.803767 | 2.98E-49 | -1.69E-46 |
| MS.gene056761 | MS.gene84704 | 0.815516 | 8.88E-52 | -1.69E-46 |
| MS.gene057014 | MS.gene84704 | 0.827157 | 1.83E-54 | -1.69E-46 |
| MS.gene058068 | MS.gene84704 | 0.832957 | 7.03E-56 | -1.69E-46 |
| MS.gene058099 | MS.gene84704 | 0.831181 | 1.93E-55 | -1.69E-46 |
| MS.gene058109 | MS.gene84704 | 0.815735 | 7.94E-52 | -1.69E-46 |
| MS.gene058111 | MS.gene84704 | 0.854644 | 1.07E-61 | -1.69E-46 |
| MS.gene058171 | MS.gene84704 | 0.821021 | 5.04E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene84704 | 0.814594 | 1.42E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene84704 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene058268 | MS.gene84704 | 0.819615 | 1.06E-52 | -1.69E-46 |
| MS.gene058269 | MS.gene84704 | 0.809337 | 1.99E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene84704 | 0.871868 | 4.92E-67 | -1.69E-46 |
| MS.gene058494 | MS.gene84704 | 0.834143 | 3.55E-56 | -1.69E-46 |
| MS.gene058628 | MS.gene84704 | 0.858938 | 5.84E-63 | -1.69E-46 |
| MS.gene058629 | MS.gene84704 | 0.853515 | 2.27E-61 | -1.69E-46 |
| MS.gene058630 | MS.gene84704 | 0.864071 | 1.58E-64 | -1.69E-46 |
| MS.gene059022 | MS.gene84704 | -0.804456 | 2.14E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene84704 | 0.81264 | 3.83E-51 | -1.69E-46 |
| MS.gene059690 | MS.gene84704 | 0.833102 | 6.47E-56 | -1.69E-46 |
| MS.gene059694 | MS.gene84704 | 0.837824 | 4.14E-57 | -1.69E-46 |
| MS.gene059697 | MS.gene84704 | 0.833646 | 4.73E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene84704 | -0.823038 | 1.72E-53 | -1.69E-46 |
| MS.gene060595 | MS.gene84704 | 0.83327 | 5.87E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene84704 | -0.819034 | 1.44E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene84704 | -0.806385 | 8.44E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84704.t1 | MTR_3g086830 | 94.156 | 154 | 9 | 0 | 1 | 154 | 1 | 154 | 1.32e-81 | 238 |
| MS.gene84704.t1 | MTR_8g089920 | 43.137 | 153 | 75 | 4 | 4 | 154 | 1 | 143 | 6.05e-33 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGGTAATTGTTTATCATT+TGG | 0.298328 | 3.4:-80831012 | None:intergenic |
| AGGAATCTGTAGTGAATTCT+AGG | 0.331571 | 3.4:+80831391 | MS.gene84704:CDS |
| AGGGATGTTGGTGGAGGTAG+AGG | 0.333746 | 3.4:+80831312 | MS.gene84704:CDS |
| GAAGAAAGAAAGATGGCTTT+TGG | 0.373560 | 3.4:+80831334 | MS.gene84704:CDS |
| TTGTTTCGAGGAACCGAGTC+AGG | 0.374583 | 3.4:+80831286 | MS.gene84704:CDS |
| AGGTACTCAAATTGAGGAAA+AGG | 0.393008 | 3.4:+80831361 | MS.gene84704:CDS |
| GAGAGAATCAATAGGGTTCT+TGG | 0.399060 | 3.4:-80831079 | None:intergenic |
| TGAGGAGAAGCAGGAGAAGA+AGG | 0.411004 | 3.4:-80831114 | None:intergenic |
| CAATAGTAGAGAGAATCAAT+AGG | 0.415959 | 3.4:-80831087 | None:intergenic |
| TTTGTTGAGGCATCATCAAT+AGG | 0.416519 | 3.4:-80831138 | None:intergenic |
| GATGATGCCTCAACAAATCA+TGG | 0.439486 | 3.4:+80831144 | MS.gene84704:CDS |
| TAGTGAATTCTAGGACTTGA+AGG | 0.449666 | 3.4:+80831400 | MS.gene84704:CDS |
| GGTCCAAGGTACTCAAATTG+AGG | 0.456858 | 3.4:+80831355 | MS.gene84704:CDS |
| TTTCCTCAATTTGAGTACCT+TGG | 0.458595 | 3.4:-80831358 | None:intergenic |
| AGGGAGAGGGATGTTGGTGG+AGG | 0.478556 | 3.4:+80831306 | MS.gene84704:CDS |
| AGTCGCTTGCCGTTGTTTCG+AGG | 0.489139 | 3.4:+80831274 | MS.gene84704:CDS |
| TCAATAGGTTGAGGAGAAGC+AGG | 0.490038 | 3.4:-80831123 | None:intergenic |
| GGTACTCAAATTGAGGAAAA+GGG | 0.507988 | 3.4:+80831362 | MS.gene84704:CDS |
| TGTCATACAAGAAGCAGAAC+CGG | 0.515419 | 3.4:-80831217 | None:intergenic |
| AATTAGAAGCAACAAGAGTA+AGG | 0.516499 | 3.4:+80830977 | MS.gene84704:CDS |
| GGTAGAGGAAGAAAGAAAGA+TGG | 0.520786 | 3.4:+80831327 | MS.gene84704:CDS |
| CGAGGAACCGAGTCAGGGAG+AGG | 0.521049 | 3.4:+80831292 | MS.gene84704:CDS |
| AATAGTAGAGAGAATCAATA+GGG | 0.528508 | 3.4:-80831086 | None:intergenic |
| CAACATCCCTCTCCCTGACT+CGG | 0.533206 | 3.4:-80831299 | None:intergenic |
| CGAGTCAGGGAGAGGGATGT+TGG | 0.536071 | 3.4:+80831300 | MS.gene84704:CDS |
| CAATAATGAATCAGATGAAG+TGG | 0.549086 | 3.4:+80830950 | None:intergenic |
| GTCAGGGAGAGGGATGTTGG+TGG | 0.559065 | 3.4:+80831303 | MS.gene84704:CDS |
| GAGGCATCATCAATAGGTTG+AGG | 0.568712 | 3.4:-80831132 | None:intergenic |
| GAGTTTGTCTCTTAAACAAG+AGG | 0.578724 | 3.4:-80831043 | None:intergenic |
| CCAAATGATAAACAATTACC+AGG | 0.580717 | 3.4:+80831012 | MS.gene84704:CDS |
| GAGGAACCGAGTCAGGGAGA+GGG | 0.581827 | 3.4:+80831293 | MS.gene84704:CDS |
| ATCGTAGTAGACACTTTCGC+AGG | 0.593355 | 3.4:+80831178 | MS.gene84704:CDS |
| TGAAGACCCATGATTTGTTG+AGG | 0.607126 | 3.4:-80831151 | None:intergenic |
| AGGAATGCTTCACAAGCGAC+CGG | 0.610619 | 3.4:+80831198 | MS.gene84704:CDS |
| ATGATGCCTCAACAAATCAT+GGG | 0.612329 | 3.4:+80831145 | MS.gene84704:CDS |
| ATTGAGGAAAAGGGATACAG+AGG | 0.620071 | 3.4:+80831371 | MS.gene84704:CDS |
| TGTTTCGAGGAACCGAGTCA+GGG | 0.624425 | 3.4:+80831287 | MS.gene84704:CDS |
| AAACAAGAGGAACAAACACC+TGG | 0.650993 | 3.4:-80831030 | None:intergenic |
| GACTCGGTTCCTCGAAACAA+CGG | 0.676399 | 3.4:-80831283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGTAGAGAGAATCAATA+GGG | - | chr3.4:80831089-80831108 | None:intergenic | 25.0% |
| AATTAGAAGCAACAAGAGTA+AGG | + | chr3.4:80830977-80830996 | MS.gene84704:CDS | 30.0% | |
| CAATAGTAGAGAGAATCAAT+AGG | - | chr3.4:80831090-80831109 | None:intergenic | 30.0% | |
| CCAAATGATAAACAATTACC+AGG | + | chr3.4:80831012-80831031 | MS.gene84704:CDS | 30.0% | |
| CCTGGTAATTGTTTATCATT+TGG | - | chr3.4:80831015-80831034 | None:intergenic | 30.0% | |
| AGGAATCTGTAGTGAATTCT+AGG | + | chr3.4:80831391-80831410 | MS.gene84704:CDS | 35.0% | |
| AGGTACTCAAATTGAGGAAA+AGG | + | chr3.4:80831361-80831380 | MS.gene84704:CDS | 35.0% | |
| ATGATGCCTCAACAAATCAT+GGG | + | chr3.4:80831145-80831164 | MS.gene84704:CDS | 35.0% | |
| GAGTTTGTCTCTTAAACAAG+AGG | - | chr3.4:80831046-80831065 | None:intergenic | 35.0% | |
| GGTACTCAAATTGAGGAAAA+GGG | + | chr3.4:80831362-80831381 | MS.gene84704:CDS | 35.0% | |
| TTTCCTCAATTTGAGTACCT+TGG | - | chr3.4:80831361-80831380 | None:intergenic | 35.0% | |
| ! | GAAGAAAGAAAGATGGCTTT+TGG | + | chr3.4:80831334-80831353 | MS.gene84704:CDS | 35.0% |
| ! | TTTGTTGAGGCATCATCAAT+AGG | - | chr3.4:80831141-80831160 | None:intergenic | 35.0% |
| AAACAAGAGGAACAAACACC+TGG | - | chr3.4:80831033-80831052 | None:intergenic | 40.0% | |
| ATTGAGGAAAAGGGATACAG+AGG | + | chr3.4:80831371-80831390 | MS.gene84704:CDS | 40.0% | |
| GAGAGAATCAATAGGGTTCT+TGG | - | chr3.4:80831082-80831101 | None:intergenic | 40.0% | |
| GATGATGCCTCAACAAATCA+TGG | + | chr3.4:80831144-80831163 | MS.gene84704:CDS | 40.0% | |
| GGTAGAGGAAGAAAGAAAGA+TGG | + | chr3.4:80831327-80831346 | MS.gene84704:CDS | 40.0% | |
| TGTCATACAAGAAGCAGAAC+CGG | - | chr3.4:80831220-80831239 | None:intergenic | 40.0% | |
| !! | TGAAGACCCATGATTTGTTG+AGG | - | chr3.4:80831154-80831173 | None:intergenic | 40.0% |
| ATCGTAGTAGACACTTTCGC+AGG | + | chr3.4:80831178-80831197 | MS.gene84704:CDS | 45.0% | |
| ! | GAAAGATGGCTTTTGGTCCA+AGG | + | chr3.4:80831341-80831360 | MS.gene84704:CDS | 45.0% |
| ! | GAGGCATCATCAATAGGTTG+AGG | - | chr3.4:80831135-80831154 | None:intergenic | 45.0% |
| ! | GGTCCAAGGTACTCAAATTG+AGG | + | chr3.4:80831355-80831374 | MS.gene84704:CDS | 45.0% |
| ! | TCAATAGGTTGAGGAGAAGC+AGG | - | chr3.4:80831126-80831145 | None:intergenic | 45.0% |
| AGGAATGCTTCACAAGCGAC+CGG | + | chr3.4:80831198-80831217 | MS.gene84704:CDS | 50.0% | |
| TGAGGAGAAGCAGGAGAAGA+AGG | - | chr3.4:80831117-80831136 | None:intergenic | 50.0% | |
| TGTTTCGAGGAACCGAGTCA+GGG | + | chr3.4:80831287-80831306 | MS.gene84704:CDS | 50.0% | |
| TTGTTTCGAGGAACCGAGTC+AGG | + | chr3.4:80831286-80831305 | MS.gene84704:CDS | 50.0% | |
| ! | GACTCGGTTCCTCGAAACAA+CGG | - | chr3.4:80831286-80831305 | None:intergenic | 50.0% |
| CAACATCCCTCTCCCTGACT+CGG | - | chr3.4:80831302-80831321 | None:intergenic | 55.0% | |
| ! | AGGGATGTTGGTGGAGGTAG+AGG | + | chr3.4:80831312-80831331 | MS.gene84704:CDS | 55.0% |
| ! | AGTCGCTTGCCGTTGTTTCG+AGG | + | chr3.4:80831274-80831293 | MS.gene84704:CDS | 55.0% |
| CGAGTCAGGGAGAGGGATGT+TGG | + | chr3.4:80831300-80831319 | MS.gene84704:CDS | 60.0% | |
| GAGGAACCGAGTCAGGGAGA+GGG | + | chr3.4:80831293-80831312 | MS.gene84704:CDS | 60.0% | |
| GTCAGGGAGAGGGATGTTGG+TGG | + | chr3.4:80831303-80831322 | MS.gene84704:CDS | 60.0% | |
| ! | AGGGAGAGGGATGTTGGTGG+AGG | + | chr3.4:80831306-80831325 | MS.gene84704:CDS | 60.0% |
| CGAGGAACCGAGTCAGGGAG+AGG | + | chr3.4:80831292-80831311 | MS.gene84704:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 80830955 | 80831419 | 80830955 | ID=MS.gene84704 |
| chr3.4 | mRNA | 80830955 | 80831419 | 80830955 | ID=MS.gene84704.t1;Parent=MS.gene84704 |
| chr3.4 | exon | 80830955 | 80831419 | 80830955 | ID=MS.gene84704.t1.exon1;Parent=MS.gene84704.t1 |
| chr3.4 | CDS | 80830955 | 80831419 | 80830955 | ID=cds.MS.gene84704.t1;Parent=MS.gene84704.t1 |
| Gene Sequence |
| Protein sequence |
