AlfalfaGEDB | MS.gene86615

M. sativa cultivar XinJiangDaYe / MS.gene86615

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86615.t1	GAU50255.1	73.9	153	37	1	1	153	74	223	2.90E-48	201.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86615.t1	P93835	48.4	155	72	2	1	151	63	213	1.9e-27	123.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86615.t1	A0A2Z6PIX3	73.9	153	37	1	1	153	74	223	2.1e-48	201.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene79315	MS.gene86615	PPI
MS.gene86613	MS.gene86615	PPI
MS.gene86615	MS.gene86607	PPI
MS.gene86615	MS.gene49252	PPI
MS.gene86615	MS.gene30840	PPI
MS.gene49255	MS.gene86615	PPI
MS.gene30848	MS.gene86615	PPI
MS.gene86615	MS.gene30851	PPI
MS.gene86615	MS.gene86588	PPI
MS.gene86615	MS.gene86611	PPI
MS.gene006341	MS.gene86615	PPI
MS.gene86615	MS.gene86601	PPI
MS.gene86615	MS.gene34447	PPI
MS.gene30842	MS.gene86615	PPI
MS.gene86615	MS.gene62758	PPI
MS.gene86615	MS.gene49250	PPI
MS.gene86615	MS.gene86591	PPI
MS.gene86615	MS.gene30853	PPI
MS.gene86615	MS.gene79330	PPI
MS.gene30852	MS.gene86615	PPI
MS.gene79322	MS.gene86615	PPI
MS.gene86615	MS.gene43297	PPI
MS.gene86615	MS.gene86610	PPI
MS.gene79326	MS.gene86615	PPI
MS.gene79327	MS.gene86615	PPI
MS.gene86615	MS.gene86596	PPI
MS.gene86615	MS.gene86594	PPI
MS.gene86615	MS.gene62760	PPI
MS.gene86615	MS.gene49040	PPI
MS.gene86615	MS.gene30844	PPI
MS.gene86615	MS.gene79314	PPI
MS.gene86615	MS.gene41386	PPI
MS.gene86615	MS.gene79320	PPI
MS.gene86615	MS.gene38658	PPI
MS.gene86615	MS.gene79316	PPI
MS.gene86615	MS.gene79328	PPI
MS.gene30841	MS.gene86615	PPI
MS.gene86615	MS.gene79327	PPI
MS.gene86597	MS.gene86615	PPI
MS.gene86615	MS.gene79318	PPI
MS.gene86615	MS.gene79321	PPI
MS.gene86615	MS.gene86599	PPI
MS.gene86615	MS.gene30845	PPI
MS.gene86615	MS.gene86612	PPI
MS.gene86615	MS.gene006341	PPI
MS.gene86615	MS.gene49249	PPI
MS.gene86615	MS.gene49253	PPI
MS.gene86615	MS.gene43298	PPI
MS.gene86615	MS.gene86595	PPI
MS.gene86615	MS.gene79310	PPI
MS.gene86615	MS.gene62759	PPI
MS.gene86615	MS.gene86608	PPI
MS.gene86615	MS.gene86618	PPI
MS.gene86615	MS.gene79307	PPI
MS.gene86615	MS.gene49251	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86615.t1	MTR_6g466130	78.571	154	28	2	1	153	81	230	1.91e-68	207
MS.gene86615.t1	MTR_6g466000	66.049	162	38	3	1	151	72	227	3.29e-65	198
MS.gene86615.t1	MTR_6g465530	70.199	151	45	0	1	151	48	198	1.16e-64	196
MS.gene86615.t1	MTR_6g465460	66.897	145	41	1	1	145	69	206	6.53e-64	197
MS.gene86615.t1	MTR_6g465420	67.347	147	41	3	1	145	69	210	4.84e-63	194
MS.gene86615.t1	MTR_6g465430	68.276	145	40	1	1	145	69	207	1.75e-60	187
MS.gene86615.t1	MTR_6g465450	69.595	148	37	3	1	145	69	211	6.32e-60	186
MS.gene86615.t1	MTR_6g465510	70.807	161	37	2	1	151	48	208	6.78e-60	184
MS.gene86615.t1	MTR_6g465690	68.707	147	38	3	1	145	78	218	1.02e-59	186
MS.gene86615.t1	MTR_6g465850	54.327	208	36	4	1	153	78	281	2.75e-56	177
MS.gene86615.t1	MTR_6g466020	61.745	149	30	3	1	148	58	180	5.35e-49	155
MS.gene86615.t1	MTR_1g101600	56.209	153	61	1	1	153	56	202	2.05e-48	155
MS.gene86615.t1	MTR_6g465990	53.704	162	38	4	1	151	58	193	1.32e-37	127
MS.gene86615.t1	MTR_4g102660	44.910	167	71	5	1	153	61	220	1.13e-28	105
MS.gene86615.t1	MTR_5g010930	42.208	154	83	3	5	153	74	226	4.68e-27	101
MS.gene86615.t1	MTR_5g010910	39.474	152	88	2	5	153	74	224	2.02e-26	99.8
MS.gene86615.t1	MTR_5g010940	39.474	152	88	2	5	153	79	229	2.14e-26	99.8
MS.gene86615.t1	MTR_3g110205	58.824	68	28	0	1	68	58	125	2.75e-20	82.8
MS.gene86615.t1	MTR_8g006410	61.667	60	23	0	1	60	67	126	4.27e-19	80.1
MS.gene86615.t1	MTR_2g101340	54.321	81	37	0	1	81	56	136	1.01e-18	79.3
MS.gene86615.t1	MTR_7g046260	60.000	60	24	0	1	60	76	135	2.08e-18	79.0
MS.gene86615.t1	MTR_5g012910	58.333	60	25	0	1	60	43	102	1.78e-17	75.5
MS.gene86615.t1	MTR_6g088405	58.333	60	25	0	1	60	59	118	2.25e-17	75.9
MS.gene86615.t1	MTR_8g013860	58.333	60	25	0	1	60	62	121	2.42e-17	75.5
MS.gene86615.t1	MTR_8g102800	58.333	60	25	0	1	60	52	111	4.03e-17	75.5
MS.gene86615.t1	MTR_4g100650	60.000	60	24	0	1	60	89	148	3.80e-16	73.2
MS.gene86615.t1	MTR_4g102670	56.667	60	26	0	1	60	29	88	4.57e-16	71.2
MS.gene86615.t1	MTR_7g117690	51.667	60	29	0	1	60	43	102	1.40e-15	70.1
MS.gene86615.t1	MTR_4g035430	50.746	67	33	0	1	67	47	113	1.96e-15	70.9
MS.gene86615.t1	MTR_2g085015	31.515	165	93	6	3	150	65	226	3.89e-15	70.1
MS.gene86615.t1	MTR_8g027465	53.968	63	26	2	1	60	54	116	1.14e-13	65.1
MS.gene86615.t1	MTR_5g008470	43.662	71	40	0	1	71	90	160	8.32e-13	63.2
MS.gene86615.t1	MTR_6g088425	56.716	67	29	0	1	67	64	130	9.26e-13	63.9
MS.gene86615.t1	MTR_5g008550	47.887	71	37	0	1	71	36	106	2.55e-12	62.0
MS.gene86615.t1	MTR_1g060910	31.288	163	91	8	2	149	15	171	3.21e-12	61.2
MS.gene86615.t1	MTR_5g008590	46.154	91	48	1	1	90	26	116	2.79e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86615.t1	AT4G25470	50.932	161	59	6	1	151	66	216	1.20e-38	130
MS.gene86615.t1	AT4G25490	52.201	159	60	4	1	151	63	213	3.22e-37	127
MS.gene86615.t1	AT5G51990	48.765	162	64	5	1	151	69	222	2.67e-36	125
MS.gene86615.t1	AT4G25480	52.500	160	58	5	1	151	66	216	8.12e-36	123
MS.gene86615.t1	AT1G63030	40.645	155	70	4	1	151	45	181	2.94e-30	108
MS.gene86615.t1	AT1G63030	40.645	155	70	4	1	151	45	181	2.94e-30	108
MS.gene86615.t1	AT1G12610	41.667	168	76	5	1	151	45	207	3.77e-30	108
MS.gene86615.t1	AT4G16750	65.000	60	21	0	1	60	55	114	1.16e-20	83.6
MS.gene86615.t1	AT2G35700	65.000	60	21	0	1	60	60	119	1.26e-20	84.0
MS.gene86615.t1	AT5G25810	58.209	67	28	0	1	67	51	117	4.85e-20	82.8
MS.gene86615.t1	AT5G11590	58.209	67	28	0	1	67	66	132	2.66e-19	81.3
MS.gene86615.t1	AT4G32800	53.731	67	31	0	1	67	34	100	5.23e-19	80.1
MS.gene86615.t1	AT2G44940	59.701	67	27	0	1	67	115	181	8.81e-19	80.9
MS.gene86615.t1	AT3G16280	44.444	126	66	2	1	125	76	198	1.44e-17	76.6
MS.gene86615.t1	AT2G25820	60.000	60	24	0	1	60	41	100	1.66e-17	76.3
MS.gene86615.t1	AT3G16280	44.444	126	66	2	1	125	94	216	1.69e-17	77.0
MS.gene86615.t1	AT3G60490	58.333	60	25	0	1	60	86	145	4.14e-17	75.9
MS.gene86615.t1	AT2G36450	40.123	162	77	6	1	151	31	183	2.68e-16	72.4
MS.gene86615.t1	AT1G01250	51.667	60	29	0	1	60	60	119	2.23e-14	67.4
MS.gene86615.t1	AT1G71450	32.886	149	85	3	1	149	39	172	2.81e-14	67.0
MS.gene86615.t1	AT1G21910	43.590	78	37	1	1	71	65	142	3.12e-12	62.4
MS.gene86615.t1	AT5G52020	35.329	167	78	3	1	151	79	231	4.37e-12	62.0
MS.gene86615.t1	AT1G33760	50.667	75	37	0	1	75	35	109	5.68e-12	60.8
MS.gene86615.t1	AT1G77200	58.333	60	25	0	1	60	58	117	1.13e-11	60.8

Find 33 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATCTTAGTCTTCTTGTT+AGG	0.284793	6.1:-50583654	None:intergenic
GCTCCCTATTCCTGCCTCTA+TGG	0.317419	6.1:+50583785	MS.gene86615:CDS
GGAGCAGCTTATGTTTAGTA+TGG	0.317437	6.1:+50583917	MS.gene86615:CDS
TCCAGAGTCGTTGAGGAATA+TGG	0.323237	6.1:+50583986	MS.gene86615:CDS
GTCTCCATAGAGGCAGGAAT+AGG	0.423149	6.1:-50583789	None:intergenic
AAGATATTGAGGTGTCACTA+TGG	0.433062	6.1:+50584069	MS.gene86615:CDS
TCTCCATAGAGGCAGGAATA+GGG	0.436980	6.1:-50583788	None:intergenic
CTAACAAGAAGACTAAGATA+TGG	0.448708	6.1:+50583655	MS.gene86615:CDS
CTAGTCAATAAAATATTGTC+TGG	0.451324	6.1:-50583858	None:intergenic
GCCATATTCCTCAACGACTC+TGG	0.459205	6.1:-50583987	None:intergenic
AGCTCATGATGTCGCGGCAT+TGG	0.460891	6.1:+50583713	MS.gene86615:CDS
TCAACTTTGCAGACTCTGCC+TGG	0.466308	6.1:+50583760	MS.gene86615:CDS
TGCAGACTTTCAAGATATTG+AGG	0.482224	6.1:+50584058	MS.gene86615:CDS
AAGCTGCTCCTCTGTGGCAT+TGG	0.491565	6.1:-50583904	None:intergenic
GCTTATGTTTAGTATGGAAG+AGG	0.512417	6.1:+50583923	MS.gene86615:CDS
GGCGGCAGCAGAAGCCGCAG+AGG	0.519491	6.1:+50583827	MS.gene86615:CDS
CATGCTCAAAGCTATGTGTA+GGG	0.523141	6.1:-50584019	None:intergenic
AGAGTCTGCAAAGTTGAGAC+AGG	0.526773	6.1:-50583754	None:intergenic
GGAGACAAAAGATATACAAA+AGG	0.529482	6.1:+50583806	MS.gene86615:CDS
GGCAGGAATAGGGAGCCGCC+AGG	0.530263	6.1:-50583778	None:intergenic
TCATGCTCAAAGCTATGTGT+AGG	0.531088	6.1:-50584020	None:intergenic
AGCTTTGAGCATGAATATCA+AGG	0.559097	6.1:+50584029	MS.gene86615:CDS
AAGAAGACTAAGATATGGCT+AGG	0.575055	6.1:+50583660	MS.gene86615:CDS
GACAAAAGATATACAAAAGG+CGG	0.601517	6.1:+50583809	MS.gene86615:CDS
GTCTGGTTTGAAAGCCTCTG+CGG	0.608883	6.1:-50583841	None:intergenic
TGAACATTCCAGAGTCGTTG+AGG	0.613398	6.1:+50583979	MS.gene86615:CDS
GTCGCGGCATTGGCATTGAG+AGG	0.626845	6.1:+50583723	MS.gene86615:CDS
AACACGAGCTCATGATGTCG+CGG	0.638651	6.1:+50583707	MS.gene86615:CDS
ATGCTCAAAGCTATGTGTAG+GGG	0.666315	6.1:-50584018	None:intergenic
AAACATAAGCTGCTCCTCTG+TGG	0.667851	6.1:-50583910	None:intergenic
ATTGACTAGTGATATTGACA+CGG	0.675849	6.1:+50583872	MS.gene86615:CDS
TGTAGCTGCCAATGCCACAG+AGG	0.716804	6.1:+50583896	MS.gene86615:CDS
ACTTTGCAGACTCTGCCTGG+CGG	0.759244	6.1:+50583763	MS.gene86615:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTAGTCAATAAAATATTGTC+TGG	-	chr6.1:50583861-50583880	None:intergenic	25.0%
	AGAAGAAGAAGAAGAAAAAG+AGG	+	chr6.1:50583947-50583966	MS.gene86615:CDS	30.0%
	ATTGACTAGTGATATTGACA+CGG	+	chr6.1:50583872-50583891	MS.gene86615:CDS	30.0%
	CTAACAAGAAGACTAAGATA+TGG	+	chr6.1:50583655-50583674	MS.gene86615:CDS	30.0%
	GACAAAAGATATACAAAAGG+CGG	+	chr6.1:50583809-50583828	MS.gene86615:CDS	30.0%
	GGAGACAAAAGATATACAAA+AGG	+	chr6.1:50583806-50583825	MS.gene86615:CDS	30.0%
!	CATATCTTAGTCTTCTTGTT+AGG	-	chr6.1:50583657-50583676	None:intergenic	30.0%
!	TATATCTTTTGTCTCCATAG+AGG	-	chr6.1:50583802-50583821	None:intergenic	30.0%
	AAGAAGACTAAGATATGGCT+AGG	+	chr6.1:50583660-50583679	MS.gene86615:CDS	35.0%
	AGAAGAAGAAGAAAAAGAGG+AGG	+	chr6.1:50583950-50583969	MS.gene86615:CDS	35.0%
	GCTTATGTTTAGTATGGAAG+AGG	+	chr6.1:50583923-50583942	MS.gene86615:CDS	35.0%
	TGCAGACTTTCAAGATATTG+AGG	+	chr6.1:50584058-50584077	MS.gene86615:CDS	35.0%
!!	AAGATATTGAGGTGTCACTA+TGG	+	chr6.1:50584069-50584088	MS.gene86615:CDS	35.0%
!!	AGCTTTGAGCATGAATATCA+AGG	+	chr6.1:50584029-50584048	MS.gene86615:CDS	35.0%
	ATGCTCAAAGCTATGTGTAG+GGG	-	chr6.1:50584021-50584040	None:intergenic	40.0%
	CATGCTCAAAGCTATGTGTA+GGG	-	chr6.1:50584022-50584041	None:intergenic	40.0%
	GGAGCAGCTTATGTTTAGTA+TGG	+	chr6.1:50583917-50583936	MS.gene86615:CDS	40.0%
	TCATGCTCAAAGCTATGTGT+AGG	-	chr6.1:50584023-50584042	None:intergenic	40.0%
!	AACTTTTCGAACGGTTGAGA+TGG	+	chr6.1:50583683-50583702	MS.gene86615:CDS	40.0%
!	ATGGCTAGGAACTTTTCGAA+CGG	+	chr6.1:50583674-50583693	MS.gene86615:CDS	40.0%
	AAACATAAGCTGCTCCTCTG+TGG	-	chr6.1:50583913-50583932	None:intergenic	45.0%
	AGAGTCTGCAAAGTTGAGAC+AGG	-	chr6.1:50583757-50583776	None:intergenic	45.0%
	TCCAGAGTCGTTGAGGAATA+TGG	+	chr6.1:50583986-50584005	MS.gene86615:CDS	45.0%
	TCTCCATAGAGGCAGGAATA+GGG	-	chr6.1:50583791-50583810	None:intergenic	45.0%
	TGAACATTCCAGAGTCGTTG+AGG	+	chr6.1:50583979-50583998	MS.gene86615:CDS	45.0%
!	TCTTTTGTCTCCATAGAGGC+AGG	-	chr6.1:50583798-50583817	None:intergenic	45.0%
	AACACGAGCTCATGATGTCG+CGG	+	chr6.1:50583707-50583726	MS.gene86615:CDS	50.0%
	GCCATATTCCTCAACGACTC+TGG	-	chr6.1:50583990-50584009	None:intergenic	50.0%
	GTCTCCATAGAGGCAGGAAT+AGG	-	chr6.1:50583792-50583811	None:intergenic	50.0%
	TCAACTTTGCAGACTCTGCC+TGG	+	chr6.1:50583760-50583779	MS.gene86615:CDS	50.0%
!	GTCTGGTTTGAAAGCCTCTG+CGG	-	chr6.1:50583844-50583863	None:intergenic	50.0%
	AAGCTGCTCCTCTGTGGCAT+TGG	-	chr6.1:50583907-50583926	None:intergenic	55.0%
	ACTTTGCAGACTCTGCCTGG+CGG	+	chr6.1:50583763-50583782	MS.gene86615:CDS	55.0%
	AGCTCATGATGTCGCGGCAT+TGG	+	chr6.1:50583713-50583732	MS.gene86615:CDS	55.0%
	GCTCCCTATTCCTGCCTCTA+TGG	+	chr6.1:50583785-50583804	MS.gene86615:CDS	55.0%
	TGTAGCTGCCAATGCCACAG+AGG	+	chr6.1:50583896-50583915	MS.gene86615:CDS	55.0%
!!	GTCGCGGCATTGGCATTGAG+AGG	+	chr6.1:50583723-50583742	MS.gene86615:CDS	60.0%
	GGCAGGAATAGGGAGCCGCC+AGG	-	chr6.1:50583781-50583800	None:intergenic	70.0%
	GGCGGCAGCAGAAGCCGCAG+AGG	+	chr6.1:50583827-50583846	MS.gene86615:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	50583645	50584106	50583645	ID=MS.gene86615
chr6.1	mRNA	50583645	50584106	50583645	ID=MS.gene86615.t1;Parent=MS.gene86615
chr6.1	exon	50583645	50584106	50583645	ID=MS.gene86615.t1.exon1;Parent=MS.gene86615.t1
chr6.1	CDS	50583645	50584106	50583645	ID=cds.MS.gene86615.t1;Parent=MS.gene86615.t1

Gene Sequence

>MS.gene86615

ATGAGGGAGCCTAACAAGAAGACTAAGATATGGCTAGGAACTTTTCGAACGGTTGAGATGGCAACACGAGCTCATGATGTCGCGGCATTGGCATTGAGAGGTCGTTACGCCTGTCTCAACTTTGCAGACTCTGCCTGGCGGCTCCCTATTCCTGCCTCTATGGAGACAAAAGATATACAAAAGGCGGCAGCAGAAGCCGCAGAGGCTTTCAAACCAGACAATATTTTATTGACTAGTGATATTGACACGGTTGTAGCTGCCAATGCCACAGAGGAGCAGCTTATGTTTAGTATGGAAGAGGAAGAAGAAGAAGAAGAAAAAGAGGAGGAAGTGTTGAACATTCCAGAGTCGTTGAGGAATATGGCTCTAATGTCCCCTACACATAGCTTTGAGCATGAATATCAAGGTATTGATGCAGACTTTCAAGATATTGAGGTGTCACTATGGAGTTTTTCAATTTGA

Protein sequence

>MS.gene86615.t1

MREPNKKTKIWLGTFRTVEMATRAHDVAALALRGRYACLNFADSAWRLPIPASMETKDIQKAAAEAAEAFKPDNILLTSDIDTVVAANATEEQLMFSMEEEEEEEEKEEEVLNIPESLRNMALMSPTHSFEHEYQGIDADFQDIEVSLWSFSI