Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86678.t1 | XP_003622081.1 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 9.80E-93 | 349.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86678.t1 | B7FIQ2 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 7.1e-93 | 349.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene86678 | 0.819533 | 1.10E-52 | -1.69E-46 |
| MS.gene049341 | MS.gene86678 | 0.823293 | 1.50E-53 | -1.69E-46 |
| MS.gene049382 | MS.gene86678 | 0.814058 | 1.87E-51 | -1.69E-46 |
| MS.gene049695 | MS.gene86678 | 0.809355 | 1.97E-50 | -1.69E-46 |
| MS.gene049979 | MS.gene86678 | 0.81597 | 7.04E-52 | -1.69E-46 |
| MS.gene050020 | MS.gene86678 | 0.805119 | 1.56E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene86678 | 0.804267 | 2.35E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene86678 | 0.802842 | 4.63E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene86678 | 0.816106 | 6.56E-52 | -1.69E-46 |
| MS.gene050330 | MS.gene86678 | -0.81567 | 8.21E-52 | -1.69E-46 |
| MS.gene050438 | MS.gene86678 | 0.81724 | 3.65E-52 | -1.69E-46 |
| MS.gene050586 | MS.gene86678 | 0.828214 | 1.02E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene86678 | 0.811455 | 6.94E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene86678 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene86678 | 0.841408 | 4.84E-58 | -1.69E-46 |
| MS.gene050808 | MS.gene86678 | 0.822873 | 1.88E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene86678 | 0.80355 | 3.31E-49 | -1.69E-46 |
| MS.gene050959 | MS.gene86678 | 0.801212 | 1.00E-48 | -1.69E-46 |
| MS.gene051265 | MS.gene86678 | 0.801306 | 9.58E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene86678 | 0.814541 | 1.46E-51 | -1.69E-46 |
| MS.gene051633 | MS.gene86678 | 0.809218 | 2.11E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene86678 | 0.822583 | 2.19E-53 | -1.69E-46 |
| MS.gene051663 | MS.gene86678 | 0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene051687 | MS.gene86678 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene86678 | 0.826868 | 2.14E-54 | -1.69E-46 |
| MS.gene052176 | MS.gene86678 | 0.805835 | 1.10E-49 | -1.69E-46 |
| MS.gene052256 | MS.gene86678 | 0.821946 | 3.08E-53 | -1.69E-46 |
| MS.gene052286 | MS.gene86678 | 0.808424 | 3.12E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene86678 | 0.844194 | 8.79E-59 | -1.69E-46 |
| MS.gene052376 | MS.gene86678 | 0.85201 | 6.11E-61 | -1.69E-46 |
| MS.gene052613 | MS.gene86678 | 0.810096 | 1.37E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene86678 | 0.831762 | 1.39E-55 | -1.69E-46 |
| MS.gene052856 | MS.gene86678 | 0.820746 | 5.83E-53 | -1.69E-46 |
| MS.gene052858 | MS.gene86678 | 0.816314 | 5.89E-52 | -1.69E-46 |
| MS.gene052860 | MS.gene86678 | 0.811833 | 5.75E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene86678 | 0.804322 | 2.28E-49 | -1.69E-46 |
| MS.gene053132 | MS.gene86678 | 0.818209 | 2.21E-52 | -1.69E-46 |
| MS.gene053437 | MS.gene86678 | 0.842439 | 2.58E-58 | -1.69E-46 |
| MS.gene053630 | MS.gene86678 | 0.85574 | 5.15E-62 | -1.69E-46 |
| MS.gene054185 | MS.gene86678 | 0.803555 | 3.30E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene86678 | 0.830918 | 2.24E-55 | -1.69E-46 |
| MS.gene055608 | MS.gene86678 | 0.820001 | 8.64E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene86678 | -0.852604 | 4.14E-61 | -1.69E-46 |
| MS.gene056432 | MS.gene86678 | 0.804636 | 1.96E-49 | -1.69E-46 |
| MS.gene056584 | MS.gene86678 | 0.812525 | 4.06E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene86678 | 0.805824 | 1.11E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene86678 | 0.806826 | 6.81E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene86678 | 0.80268 | 5.00E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene86678 | 0.800861 | 1.18E-48 | -1.69E-46 |
| MS.gene057260 | MS.gene86678 | 0.861674 | 8.69E-64 | -1.69E-46 |
| MS.gene058168 | MS.gene86678 | 0.829317 | 5.51E-55 | -1.69E-46 |
| MS.gene058182 | MS.gene86678 | 0.831103 | 2.02E-55 | -1.69E-46 |
| MS.gene058851 | MS.gene86678 | 0.835199 | 1.93E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene86678 | 0.850724 | 1.41E-60 | -1.69E-46 |
| MS.gene06001 | MS.gene86678 | 0.841553 | 4.43E-58 | -1.69E-46 |
| MS.gene060165 | MS.gene86678 | 0.822741 | 2.01E-53 | -1.69E-46 |
| MS.gene060404 | MS.gene86678 | 0.804684 | 1.92E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene86678 | 0.805833 | 1.10E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene86678 | 0.832122 | 1.13E-55 | -1.69E-46 |
| MS.gene060619 | MS.gene86678 | 0.865755 | 4.69E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86678.t1 | MTR_7g027020 | 99.422 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 2.29e-127 | 355 |
| MS.gene86678.t1 | MTR_7g405770 | 48.322 | 149 | 68 | 5 | 33 | 173 | 26 | 173 | 1.09e-42 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86678.t1 | AT3G26960 | 52.381 | 147 | 63 | 3 | 29 | 172 | 30 | 172 | 3.68e-54 | 169 |
| MS.gene86678.t1 | AT5G41050 | 52.448 | 143 | 65 | 1 | 33 | 172 | 29 | 171 | 9.44e-54 | 168 |
| MS.gene86678.t1 | AT5G13140 | 48.611 | 144 | 71 | 1 | 33 | 173 | 35 | 178 | 2.03e-48 | 158 |
Find 24 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGTTTCCCTAGCACTTT+TGG | 0.216215 | 7.4:+74231542 | None:intergenic |
| TCTTGCCAAGCAAATTTAAT+AGG | 0.232515 | 7.4:-74230610 | MS.gene86678:CDS |
| CAAGAGAGTTAGAGGATTCA+TGG | 0.327947 | 7.4:+74231613 | None:intergenic |
| ACCACTGCTTGCAATGTTCC+TGG | 0.329830 | 7.4:-74230580 | MS.gene86678:CDS |
| CTTGCCAAGCAAATTTAATA+GGG | 0.333086 | 7.4:-74230609 | MS.gene86678:CDS |
| AAAATAAGCAAGAGAGTTAG+AGG | 0.355066 | 7.4:+74231605 | None:intergenic |
| AACAGAACAACAAATAAGTA+TGG | 0.359972 | 7.4:-74230700 | MS.gene86678:CDS |
| ATTTGAGAGTTAGGAAATCT+TGG | 0.382666 | 7.4:+74231524 | None:intergenic |
| AACTCCCTATTAAATTTGCT+TGG | 0.406122 | 7.4:+74230605 | None:intergenic |
| ATCACACTGATTTGAGAGTT+AGG | 0.431769 | 7.4:+74231515 | None:intergenic |
| ATTGAAGTACCATCAGTTGA+TGG | 0.490136 | 7.4:-74230667 | MS.gene86678:CDS |
| AGACATAGCTACTTCTTGCC+AGG | 0.496803 | 7.4:-74231449 | MS.gene86678:intron |
| AGACCAGTGTCCTTCTTAGA+TGG | 0.511274 | 7.4:+74230472 | None:intergenic |
| AACTCTCAAATCAGTGTGAT+GGG | 0.512279 | 7.4:-74231512 | MS.gene86678:CDS |
| TAACTCTCAAATCAGTGTGA+TGG | 0.513119 | 7.4:-74231513 | MS.gene86678:CDS |
| CCTGCAAGACCAAAAGTGCT+AGG | 0.546586 | 7.4:-74231551 | MS.gene86678:CDS |
| CAAATAAGTATGGAATGTAC+AGG | 0.554772 | 7.4:-74230690 | MS.gene86678:CDS |
| ATAATTATATTCAGGTGCTG+AGG | 0.588603 | 7.4:-74230788 | MS.gene86678:intron |
| CATCTCACTCCATCAACTGA+TGG | 0.589191 | 7.4:+74230658 | None:intergenic |
| AAGAAGGACACTGGTCTATG+TGG | 0.605551 | 7.4:-74230466 | MS.gene86678:CDS |
| TCCAGGAACATTGCAAGCAG+TGG | 0.607145 | 7.4:+74230579 | None:intergenic |
| CTGCAAGACCAAAAGTGCTA+GGG | 0.608365 | 7.4:-74231550 | MS.gene86678:CDS |
| AGACCATCTAAGAAGGACAC+TGG | 0.644130 | 7.4:-74230475 | MS.gene86678:CDS |
| GATGGAGTGAGATGTGCTGA+AGG | 0.665515 | 7.4:-74230649 | MS.gene86678:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAAAAAATATATATATT+TGG | - | chr7.4:74230985-74231004 | MS.gene86678:intron | 0.0% |
| !! | AGAAATTACTATAAATTAAA+AGG | - | chr7.4:74230758-74230777 | MS.gene86678:CDS | 10.0% |
| !!! | ATGAATGTTTTTAATTTATT+TGG | + | chr7.4:74230874-74230893 | None:intergenic | 10.0% |
| !!! | TAAATCCTTTAAAAAAATAT+TGG | - | chr7.4:74230732-74230751 | MS.gene86678:CDS | 10.0% |
| !! | ATTAAAAACATTCATTAATG+TGG | - | chr7.4:74230879-74230898 | MS.gene86678:intron | 15.0% |
| !! | GTATTCTTTATAATAACAAA+AGG | + | chr7.4:74231106-74231125 | None:intergenic | 15.0% |
| !! | TTACTATAAATTAAAAGGAT+AGG | - | chr7.4:74230763-74230782 | MS.gene86678:CDS | 15.0% |
| !! | TTTCTTTCATAATTATATTC+AGG | - | chr7.4:74231274-74231293 | MS.gene86678:intron | 15.0% |
| !! | GGATTTAAAAAAGAGATTAA+TGG | - | chr7.4:74230788-74230807 | MS.gene86678:intron | 20.0% |
| !! | TATAAATTAAAAGGATAGGA+GGG | - | chr7.4:74230767-74230786 | MS.gene86678:CDS | 20.0% |
| !! | TTTAAAAAAGAGATTAATGG+TGG | - | chr7.4:74230791-74230810 | MS.gene86678:intron | 20.0% |
| !!! | CATTTTACAACTTTTTATCA+AGG | - | chr7.4:74231049-74231068 | MS.gene86678:intron | 20.0% |
| !!! | TTTTTCTGAAGACAAAATTA+GGG | - | chr7.4:74231138-74231157 | MS.gene86678:intron | 20.0% |
| !!! | TTTTTTCTGAAGACAAAATT+AGG | - | chr7.4:74231137-74231156 | MS.gene86678:intron | 20.0% |
| ! | AACAGAACAACAAATAAGTA+TGG | - | chr7.4:74231370-74231389 | MS.gene86678:intron | 25.0% |
| ! | ACACTCTATTTGAACTATTT+CGG | + | chr7.4:74230705-74230724 | None:intergenic | 25.0% |
| ! | CTATAAATTAAAAGGATAGG+AGG | - | chr7.4:74230766-74230785 | MS.gene86678:CDS | 25.0% |
| ! | GGTTATTCAAAATTATTACC+TGG | + | chr7.4:74230642-74230661 | None:intergenic | 25.0% |
| !! | AAATTTTAGACCATCTAAGA+AGG | - | chr7.4:74231588-74231607 | MS.gene86678:CDS | 25.0% |
| !! | ACTAATTTTTGTTAACTCAG+TGG | + | chr7.4:74231235-74231254 | None:intergenic | 25.0% |
| !! | TAGTTTAACTTTCGTAGAAA+TGG | + | chr7.4:74231209-74231228 | None:intergenic | 25.0% |
| !! | TTATATGTGCAAAGATGTTT+TGG | - | chr7.4:74230924-74230943 | MS.gene86678:intron | 25.0% |
| !!! | CTCGTCCAATATTTTTTTAA+AGG | + | chr7.4:74230740-74230759 | None:intergenic | 25.0% |
| AAAAAGAGATTAATGGTGGT+AGG | - | chr7.4:74230795-74230814 | MS.gene86678:intron | 30.0% | |
| AAAATAAGCAAGAGAGTTAG+AGG | + | chr7.4:74230468-74230487 | None:intergenic | 30.0% | |
| ATGCAAACAATTAACACTAG+AGG | + | chr7.4:74230663-74230682 | None:intergenic | 30.0% | |
| ATTTGAGAGTTAGGAAATCT+TGG | + | chr7.4:74230549-74230568 | None:intergenic | 30.0% | |
| CAAATAAGTATGGAATGTAC+AGG | - | chr7.4:74231380-74231399 | MS.gene86678:intron | 30.0% | |
| CTTGCCAAGCAAATTTAATA+GGG | - | chr7.4:74231461-74231480 | MS.gene86678:CDS | 30.0% | |
| TCCAAAAACTGAGTAAAGAA+AGG | + | chr7.4:74230492-74230511 | None:intergenic | 30.0% | |
| TCTTGCCAAGCAAATTTAAT+AGG | - | chr7.4:74231460-74231479 | MS.gene86678:CDS | 30.0% | |
| ! | AACTCCCTATTAAATTTGCT+TGG | + | chr7.4:74231468-74231487 | None:intergenic | 30.0% |
| ! | ACCTTTCTTTACTCAGTTTT+TGG | - | chr7.4:74230488-74230507 | MS.gene86678:CDS | 30.0% |
| ! | ATAATTATATTCAGGTGCTG+AGG | - | chr7.4:74231282-74231301 | MS.gene86678:intron | 30.0% |
| ! | TTGTGTATGTAAAGCTGTTT+TGG | + | chr7.4:74230829-74230848 | None:intergenic | 30.0% |
| !!! | GCAATTCTAAGTGTTTTGAT+TGG | - | chr7.4:74230901-74230920 | MS.gene86678:intron | 30.0% |
| AACTCTCAAATCAGTGTGAT+GGG | - | chr7.4:74230558-74230577 | MS.gene86678:CDS | 35.0% | |
| TAACTCTCAAATCAGTGTGA+TGG | - | chr7.4:74230557-74230576 | MS.gene86678:CDS | 35.0% | |
| ! | ATTGAAGTACCATCAGTTGA+TGG | - | chr7.4:74231403-74231422 | MS.gene86678:intron | 35.0% |
| !! | ATCACACTGATTTGAGAGTT+AGG | + | chr7.4:74230558-74230577 | None:intergenic | 35.0% |
| !! | GTATGTAAAGCTGTTTTGGA+CGG | + | chr7.4:74230825-74230844 | None:intergenic | 35.0% |
| ! | TTAGCAGTGCTTTTGTATCC+AGG | + | chr7.4:74231511-74231530 | None:intergenic | 40.0% |
| AGACATAGCTACTTCTTGCC+AGG | - | chr7.4:74230621-74230640 | MS.gene86678:CDS | 45.0% | |
| AGACCAGTGTCCTTCTTAGA+TGG | + | chr7.4:74231601-74231620 | None:intergenic | 45.0% | |
| AGACCATCTAAGAAGGACAC+TGG | - | chr7.4:74231595-74231614 | MS.gene86678:CDS | 45.0% | |
| CATCTCACTCCATCAACTGA+TGG | + | chr7.4:74231415-74231434 | None:intergenic | 45.0% | |
| ! | AAGAAGGACACTGGTCTATG+TGG | - | chr7.4:74231604-74231623 | MS.gene86678:CDS | 45.0% |
| ! | CTTGGTTTCCCTAGCACTTT+TGG | + | chr7.4:74230531-74230550 | None:intergenic | 45.0% |
| !! | CTGCAAGACCAAAAGTGCTA+GGG | - | chr7.4:74230520-74230539 | MS.gene86678:CDS | 45.0% |
| !!! | TATATTTTTTAAAATATATG+AGG | + | chr7.4:74230979-74230998 | None:intergenic | 5.0% |
| ACCACTGCTTGCAATGTTCC+TGG | - | chr7.4:74231490-74231509 | MS.gene86678:CDS | 50.0% | |
| GATGGAGTGAGATGTGCTGA+AGG | - | chr7.4:74231421-74231440 | MS.gene86678:intron | 50.0% | |
| TCCAGGAACATTGCAAGCAG+TGG | + | chr7.4:74231494-74231513 | None:intergenic | 50.0% | |
| !! | CCTGCAAGACCAAAAGTGCT+AGG | - | chr7.4:74230519-74230538 | MS.gene86678:CDS | 50.0% |
| !!! | CCTAGCACTTTTGGTCTTGC+AGG | + | chr7.4:74230522-74230541 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 74230459 | 74231633 | 74230459 | ID=MS.gene86678 |
| chr7.4 | mRNA | 74230459 | 74231633 | 74230459 | ID=MS.gene86678.t1;Parent=MS.gene86678 |
| chr7.4 | exon | 74231450 | 74231633 | 74231450 | ID=MS.gene86678.t1.exon1;Parent=MS.gene86678.t1 |
| chr7.4 | CDS | 74231450 | 74231633 | 74231450 | ID=cds.MS.gene86678.t1;Parent=MS.gene86678.t1 |
| chr7.4 | exon | 74230459 | 74230796 | 74230459 | ID=MS.gene86678.t1.exon2;Parent=MS.gene86678.t1 |
| chr7.4 | CDS | 74230459 | 74230796 | 74230459 | ID=cds.MS.gene86678.t1;Parent=MS.gene86678.t1 |
| Gene Sequence |
| Protein sequence |
