Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96657.t1 | XP_003614634.1 | 99.3 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 5.70E-265 | 922.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96657.t1 | Q9XF43 | 82.8 | 460 | 79 | 0 | 1 | 460 | 38 | 497 | 3.3e-229 | 795.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96657.t1 | G7K1J8 | 99.3 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 4.1e-265 | 922.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene96657 | 0.800235 | 1.58E-48 | -1.69E-46 |
| MS.gene049369 | MS.gene96657 | 0.829806 | 4.19E-55 | -1.69E-46 |
| MS.gene049907 | MS.gene96657 | 0.846068 | 2.74E-59 | -1.69E-46 |
| MS.gene050520 | MS.gene96657 | 0.835869 | 1.31E-56 | -1.69E-46 |
| MS.gene050593 | MS.gene96657 | 0.83046 | 2.90E-55 | -1.69E-46 |
| MS.gene050686 | MS.gene96657 | 0.837011 | 6.68E-57 | -1.69E-46 |
| MS.gene05101 | MS.gene96657 | 0.802551 | 5.32E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene96657 | 0.801263 | 9.78E-49 | -1.69E-46 |
| MS.gene051255 | MS.gene96657 | 0.847818 | 9.09E-60 | -1.69E-46 |
| MS.gene051387 | MS.gene96657 | 0.83115 | 1.97E-55 | -1.69E-46 |
| MS.gene051509 | MS.gene96657 | 0.802771 | 4.79E-49 | -1.69E-46 |
| MS.gene051692 | MS.gene96657 | 0.823053 | 1.70E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene96657 | 0.851592 | 8.03E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene96657 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene96657 | 0.811512 | 6.75E-51 | -1.69E-46 |
| MS.gene052999 | MS.gene96657 | 0.819259 | 1.28E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene96657 | 0.828082 | 1.10E-54 | -1.69E-46 |
| MS.gene053813 | MS.gene96657 | 0.922563 | 8.36E-89 | -1.69E-46 |
| MS.gene054010 | MS.gene96657 | 0.813805 | 2.13E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene96657 | 0.804688 | 1.92E-49 | -1.69E-46 |
| MS.gene054013 | MS.gene96657 | 0.812249 | 4.66E-51 | -1.69E-46 |
| MS.gene054376 | MS.gene96657 | 0.809167 | 2.16E-50 | -1.69E-46 |
| MS.gene054486 | MS.gene96657 | 0.808581 | 2.89E-50 | -1.69E-46 |
| MS.gene05492 | MS.gene96657 | 0.827459 | 1.55E-54 | -1.69E-46 |
| MS.gene05505 | MS.gene96657 | 0.803726 | 3.04E-49 | -1.69E-46 |
| MS.gene055647 | MS.gene96657 | 0.854225 | 1.42E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene96657 | 0.810053 | 1.40E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene96657 | 0.819485 | 1.13E-52 | -1.69E-46 |
| MS.gene055911 | MS.gene96657 | 0.824582 | 7.46E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene96657 | 0.823398 | 1.42E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene96657 | 0.853839 | 1.83E-61 | -1.69E-46 |
| MS.gene056410 | MS.gene96657 | 0.837185 | 6.03E-57 | -1.69E-46 |
| MS.gene056422 | MS.gene96657 | 0.832037 | 1.19E-55 | -1.69E-46 |
| MS.gene056511 | MS.gene96657 | 0.813458 | 2.53E-51 | -1.69E-46 |
| MS.gene056931 | MS.gene96657 | 0.811774 | 5.92E-51 | -1.69E-46 |
| MS.gene056933 | MS.gene96657 | 0.807898 | 4.04E-50 | -1.69E-46 |
| MS.gene056935 | MS.gene96657 | 0.802781 | 4.77E-49 | -1.69E-46 |
| MS.gene056942 | MS.gene96657 | 0.824043 | 9.99E-54 | -1.69E-46 |
| MS.gene057146 | MS.gene96657 | 0.80261 | 5.17E-49 | -1.69E-46 |
| MS.gene057285 | MS.gene96657 | 0.838738 | 2.41E-57 | -1.69E-46 |
| MS.gene057331 | MS.gene96657 | 0.824821 | 6.56E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene96657 | 0.82267 | 2.09E-53 | -1.69E-46 |
| MS.gene057749 | MS.gene96657 | 0.813237 | 2.83E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene96657 | 0.81403 | 1.90E-51 | -1.69E-46 |
| MS.gene058019 | MS.gene96657 | 0.82261 | 2.16E-53 | -1.69E-46 |
| MS.gene058459 | MS.gene96657 | 0.831239 | 1.87E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene96657 | 0.812664 | 3.78E-51 | -1.69E-46 |
| MS.gene058903 | MS.gene96657 | 0.849061 | 4.12E-60 | -1.69E-46 |
| MS.gene058995 | MS.gene96657 | 0.858879 | 6.08E-63 | -1.69E-46 |
| MS.gene05907 | MS.gene96657 | 0.816062 | 6.71E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene96657 | 0.801625 | 8.24E-49 | -1.69E-46 |
| MS.gene059455 | MS.gene96657 | 0.810971 | 8.84E-51 | -1.69E-46 |
| MS.gene059536 | MS.gene96657 | 0.80037 | 1.49E-48 | -1.69E-46 |
| MS.gene059674 | MS.gene96657 | 0.838291 | 3.14E-57 | -1.69E-46 |
| MS.gene059676 | MS.gene96657 | 0.819792 | 9.64E-53 | -1.69E-46 |
| MS.gene059680 | MS.gene96657 | 0.804218 | 2.40E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene96657 | 0.808404 | 3.15E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene96657 | 0.801084 | 1.06E-48 | -1.69E-46 |
| MS.gene059695 | MS.gene96657 | 0.801577 | 8.43E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene96657 | 0.8745 | 6.44E-68 | -1.69E-46 |
| MS.gene059807 | MS.gene96657 | 0.8048 | 1.82E-49 | -1.69E-46 |
| MS.gene059974 | MS.gene96657 | 0.857444 | 1.63E-62 | -1.69E-46 |
| MS.gene060005 | MS.gene96657 | 0.860157 | 2.51E-63 | -1.69E-46 |
| MS.gene060356 | MS.gene96657 | 0.809302 | 2.02E-50 | -1.69E-46 |
| MS.gene060401 | MS.gene96657 | 0.817961 | 2.51E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene96657 | 0.816631 | 5.01E-52 | -1.69E-46 |
| MS.gene060818 | MS.gene96657 | 0.804631 | 1.97E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene96657 | 0.801109 | 1.05E-48 | -1.69E-46 |
| MS.gene060837 | MS.gene96657 | 0.844511 | 7.22E-59 | -1.69E-46 |
| MS.gene061134 | MS.gene96657 | 0.807686 | 4.48E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene96657 | 0.830251 | 3.26E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96657.t1 | MTR_5g056510 | 99.348 | 460 | 3 | 0 | 1 | 460 | 37 | 496 | 0.0 | 951 |
| MS.gene96657.t1 | MTR_1g110930 | 90.000 | 460 | 46 | 0 | 1 | 460 | 37 | 496 | 0.0 | 860 |
| MS.gene96657.t1 | MTR_4g070270 | 64.758 | 454 | 160 | 0 | 1 | 454 | 49 | 502 | 0.0 | 637 |
| MS.gene96657.t1 | MTR_1g041085 | 62.640 | 447 | 166 | 1 | 15 | 460 | 72 | 518 | 0.0 | 597 |
| MS.gene96657.t1 | MTR_1g076430 | 61.521 | 447 | 171 | 1 | 15 | 460 | 66 | 512 | 0.0 | 593 |
| MS.gene96657.t1 | MTR_2g054940 | 62.613 | 444 | 165 | 1 | 15 | 457 | 65 | 508 | 0.0 | 592 |
| MS.gene96657.t1 | MTR_7g118170 | 60.486 | 453 | 170 | 4 | 9 | 455 | 80 | 529 | 0.0 | 565 |
| MS.gene96657.t1 | MTR_4g113280 | 59.326 | 445 | 167 | 3 | 15 | 457 | 54 | 486 | 0.0 | 562 |
| MS.gene96657.t1 | MTR_7g081695 | 59.513 | 452 | 170 | 2 | 1 | 451 | 51 | 490 | 0.0 | 555 |
| MS.gene96657.t1 | MTR_3g105550 | 55.034 | 447 | 190 | 3 | 19 | 454 | 90 | 536 | 0.0 | 530 |
| MS.gene96657.t1 | MTR_2g007020 | 54.015 | 411 | 185 | 3 | 47 | 455 | 44 | 452 | 1.44e-168 | 482 |
| MS.gene96657.t1 | MTR_4g054630 | 52.214 | 429 | 201 | 3 | 30 | 455 | 40 | 467 | 9.19e-159 | 457 |
| MS.gene96657.t1 | MTR_4g054415 | 52.594 | 424 | 197 | 3 | 34 | 454 | 3 | 425 | 1.48e-158 | 456 |
| MS.gene96657.t1 | MTR_6g074880 | 52.125 | 447 | 208 | 4 | 13 | 456 | 27 | 470 | 7.71e-158 | 455 |
| MS.gene96657.t1 | MTR_2g436480 | 50.353 | 425 | 207 | 3 | 33 | 454 | 28 | 451 | 2.00e-157 | 454 |
| MS.gene96657.t1 | MTR_1g102290 | 52.334 | 407 | 192 | 2 | 48 | 452 | 78 | 484 | 1.50e-156 | 452 |
| MS.gene96657.t1 | MTR_2g013740 | 53.671 | 395 | 180 | 1 | 49 | 443 | 109 | 500 | 2.63e-154 | 447 |
| MS.gene96657.t1 | MTR_7g118160 | 52.696 | 408 | 148 | 4 | 52 | 456 | 56 | 421 | 4.35e-153 | 442 |
| MS.gene96657.t1 | MTR_3g072980 | 51.582 | 411 | 196 | 3 | 44 | 452 | 74 | 483 | 8.65e-151 | 438 |
| MS.gene96657.t1 | MTR_3g072940 | 51.582 | 411 | 196 | 3 | 44 | 452 | 74 | 483 | 9.13e-151 | 438 |
| MS.gene96657.t1 | MTR_6g032965 | 52.815 | 373 | 175 | 1 | 84 | 455 | 13 | 385 | 8.65e-147 | 424 |
| MS.gene96657.t1 | MTR_6g051440 | 51.117 | 403 | 194 | 3 | 52 | 452 | 81 | 482 | 1.59e-138 | 407 |
| MS.gene96657.t1 | MTR_1g115560 | 39.394 | 429 | 230 | 6 | 48 | 451 | 28 | 451 | 3.87e-109 | 332 |
| MS.gene96657.t1 | MTR_4g062270 | 40.094 | 424 | 242 | 6 | 34 | 451 | 16 | 433 | 7.97e-109 | 330 |
| MS.gene96657.t1 | MTR_2g096210 | 40.732 | 437 | 236 | 8 | 34 | 453 | 16 | 446 | 1.52e-107 | 327 |
| MS.gene96657.t1 | MTR_4g062190 | 39.269 | 438 | 235 | 8 | 34 | 451 | 16 | 442 | 3.27e-106 | 323 |
| MS.gene96657.t1 | MTR_4g062170 | 40.046 | 432 | 239 | 7 | 34 | 451 | 16 | 441 | 9.92e-105 | 320 |
| MS.gene96657.t1 | MTR_4g054260 | 49.805 | 257 | 108 | 4 | 201 | 454 | 87 | 325 | 1.49e-78 | 248 |
| MS.gene96657.t1 | MTR_4g053905 | 56.875 | 160 | 66 | 2 | 297 | 454 | 1 | 159 | 6.74e-59 | 191 |
| MS.gene96657.t1 | MTR_7g086260 | 69.355 | 62 | 17 | 1 | 292 | 351 | 22 | 83 | 4.31e-19 | 82.0 |
| MS.gene96657.t1 | MTR_4g054265 | 37.069 | 116 | 73 | 0 | 34 | 149 | 3 | 118 | 8.69e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96657.t1 | AT1G68530 | 82.826 | 460 | 79 | 0 | 1 | 460 | 38 | 497 | 0.0 | 822 |
| MS.gene96657.t1 | AT1G25450 | 80.963 | 457 | 87 | 0 | 4 | 460 | 36 | 492 | 0.0 | 805 |
| MS.gene96657.t1 | AT1G19440 | 63.636 | 451 | 164 | 0 | 2 | 452 | 62 | 512 | 0.0 | 635 |
| MS.gene96657.t1 | AT2G16280 | 64.317 | 454 | 162 | 0 | 1 | 454 | 57 | 510 | 0.0 | 632 |
| MS.gene96657.t1 | AT4G34510 | 60.475 | 463 | 174 | 3 | 1 | 454 | 23 | 485 | 0.0 | 588 |
| MS.gene96657.t1 | AT2G26640 | 60.135 | 444 | 176 | 1 | 15 | 457 | 63 | 506 | 0.0 | 575 |
| MS.gene96657.t1 | AT1G04220 | 60.771 | 441 | 165 | 3 | 23 | 455 | 76 | 516 | 0.0 | 562 |
| MS.gene96657.t1 | AT5G43760 | 60.938 | 448 | 166 | 4 | 19 | 458 | 78 | 524 | 0.0 | 560 |
| MS.gene96657.t1 | AT1G01120 | 58.447 | 438 | 180 | 1 | 21 | 456 | 91 | 528 | 0.0 | 552 |
| MS.gene96657.t1 | AT1G68530 | 76.292 | 329 | 72 | 1 | 1 | 329 | 38 | 360 | 0.0 | 535 |
| MS.gene96657.t1 | AT2G15090 | 57.009 | 428 | 176 | 2 | 35 | 454 | 52 | 479 | 0.0 | 533 |
| MS.gene96657.t1 | AT4G34250 | 53.043 | 460 | 210 | 1 | 1 | 454 | 28 | 487 | 0.0 | 519 |
| MS.gene96657.t1 | AT4G34520 | 53.728 | 456 | 200 | 2 | 10 | 454 | 35 | 490 | 0.0 | 518 |
| MS.gene96657.t1 | AT2G26250 | 50.833 | 480 | 206 | 4 | 2 | 452 | 70 | 548 | 4.36e-179 | 512 |
| MS.gene96657.t1 | AT2G46720 | 53.623 | 414 | 189 | 1 | 44 | 454 | 52 | 465 | 5.06e-174 | 496 |
| MS.gene96657.t1 | AT3G10280 | 52.174 | 414 | 188 | 2 | 44 | 454 | 52 | 458 | 4.73e-166 | 476 |
| MS.gene96657.t1 | AT1G71160 | 49.652 | 431 | 213 | 3 | 23 | 452 | 19 | 446 | 1.30e-162 | 467 |
| MS.gene96657.t1 | AT5G49070 | 51.054 | 427 | 198 | 3 | 37 | 456 | 35 | 457 | 6.44e-153 | 442 |
| MS.gene96657.t1 | AT3G52160 | 45.301 | 415 | 195 | 3 | 30 | 443 | 66 | 449 | 6.52e-132 | 389 |
| MS.gene96657.t1 | AT3G52160 | 47.922 | 361 | 156 | 3 | 84 | 443 | 7 | 336 | 6.55e-123 | 362 |
| MS.gene96657.t1 | AT2G28630 | 42.080 | 423 | 223 | 8 | 48 | 451 | 27 | 446 | 2.19e-110 | 334 |
| MS.gene96657.t1 | AT2G28630 | 42.080 | 423 | 223 | 8 | 48 | 451 | 27 | 446 | 2.19e-110 | 334 |
| MS.gene96657.t1 | AT5G04530 | 40.777 | 412 | 229 | 6 | 50 | 451 | 31 | 437 | 1.20e-107 | 327 |
| MS.gene96657.t1 | AT1G07720 | 40.811 | 419 | 225 | 6 | 53 | 451 | 32 | 447 | 1.07e-106 | 325 |
| MS.gene96657.t1 | AT1G07720 | 40.811 | 419 | 225 | 6 | 53 | 451 | 75 | 490 | 1.64e-106 | 326 |
| MS.gene96657.t1 | AT5G49070 | 48.387 | 279 | 143 | 1 | 37 | 314 | 35 | 313 | 1.07e-88 | 273 |
Find 87 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACATCTTCTTCATCTTTA+TGG | 0.117063 | 5.3:-45470602 | MS.gene96657:CDS |
| ATTTAGAGCTTAACAATTTC+AGG | 0.190374 | 5.3:+45470402 | None:intergenic |
| CGTCCCTTTCGCTACTTTCA+TGG | 0.215185 | 5.3:-45471564 | MS.gene96657:CDS |
| TTCACTTCAAAAGGATCTTA+TGG | 0.218445 | 5.3:-45470895 | MS.gene96657:CDS |
| CGTCTTGCTTCTTCCATTGT+TGG | 0.258187 | 5.3:+45471416 | None:intergenic |
| GCTTCTTCCATTGTTGGTTT+AGG | 0.276327 | 5.3:+45471422 | None:intergenic |
| TGAAGAGAGGTGATAGAGTA+TGG | 0.287194 | 5.3:-45470545 | MS.gene96657:CDS |
| AGCATTAAGACACCTGTTAA+TGG | 0.300750 | 5.3:-45470465 | MS.gene96657:CDS |
| TGTTTGAAATCAGGAATATA+AGG | 0.323168 | 5.3:+45470747 | None:intergenic |
| GGTGGGATGTAATGGATTGC+TGG | 0.333428 | 5.3:+45471443 | None:intergenic |
| GAAGACAGAGAAGGTAAAGT+TGG | 0.348142 | 5.3:-45470921 | MS.gene96657:CDS |
| GAATGACGCTGCATCGATTC+GGG | 0.353166 | 5.3:-45470626 | MS.gene96657:CDS |
| ACAACAACTGCGTTTGAATT+CGG | 0.360073 | 5.3:+45471149 | None:intergenic |
| AGAGTATGGCAAATAGCTTT+TGG | 0.363430 | 5.3:-45470531 | MS.gene96657:CDS |
| ATCGTTCGAGAATTCTCATT+TGG | 0.366002 | 5.3:+45471495 | None:intergenic |
| AGCTTCAACCTCTCCGGAAT+GGG | 0.368697 | 5.3:-45471227 | MS.gene96657:CDS |
| TTTAGCCCGCGATCTTCTTC+AGG | 0.368914 | 5.3:-45471177 | MS.gene96657:CDS |
| ACCAAACTGTTTATTCAGAA+TGG | 0.377309 | 5.3:-45471069 | MS.gene96657:CDS |
| AAATCAAATATAACAACTAT+TGG | 0.388103 | 5.3:-45470852 | MS.gene96657:CDS |
| AACTCCTTCTTTGTCTGCTA+TGG | 0.395630 | 5.3:-45471285 | MS.gene96657:CDS |
| AGTTGGAATTTCACTTCAAA+AGG | 0.400364 | 5.3:-45470904 | MS.gene96657:CDS |
| TCTTCCCAAGGTCCATTAAC+AGG | 0.401821 | 5.3:+45470453 | None:intergenic |
| TCAAACGCTTGTTTGAAATC+AGG | 0.404416 | 5.3:+45470738 | None:intergenic |
| TCTTCCATTGTTGGTTTAGG+TGG | 0.408011 | 5.3:+45471425 | None:intergenic |
| CCCATTCTGAATAAACAGTT+TGG | 0.412390 | 5.3:+45471068 | None:intergenic |
| ATAAGAATGTCTATGTCCTT+AGG | 0.415605 | 5.3:+45471332 | None:intergenic |
| AGAATGACGCTGCATCGATT+CGG | 0.416495 | 5.3:-45470627 | MS.gene96657:CDS |
| TATGAAGATTATGAGAAAAC+AGG | 0.417169 | 5.3:+45471667 | None:intergenic |
| GATACCTTGTTTGCGAAAAC+CGG | 0.424265 | 5.3:-45471362 | MS.gene96657:CDS |
| ACAATTTCAGGAATATGAAC+AGG | 0.425455 | 5.3:+45470414 | None:intergenic |
| AAAGGGACGCGGCAAGTAAT+CGG | 0.425547 | 5.3:+45471578 | None:intergenic |
| TTGGTTTAGGTGGGATGTAA+TGG | 0.427468 | 5.3:+45471435 | None:intergenic |
| ATTCTCGAACGATCTGGTCT+TGG | 0.430456 | 5.3:-45471485 | MS.gene96657:CDS |
| CCAAACTGTTTATTCAGAAT+GGG | 0.431991 | 5.3:-45471068 | MS.gene96657:CDS |
| TTCATAGAACTACTACGTTT+AGG | 0.436422 | 5.3:-45471752 | MS.gene96657:CDS |
| CTTCCATTGTTGGTTTAGGT+GGG | 0.436694 | 5.3:+45471426 | None:intergenic |
| AAAAGATTGAGTATTTCAAA+AGG | 0.452047 | 5.3:+45471728 | None:intergenic |
| TTGAACTATATTGAATCAAA+AGG | 0.463579 | 5.3:-45470573 | MS.gene96657:CDS |
| GACACCTGTTAATGGACCTT+GGG | 0.463994 | 5.3:-45470457 | MS.gene96657:CDS |
| TTTGTATGTGATCAAATTGA+AGG | 0.474408 | 5.3:+45471699 | None:intergenic |
| ACCCGCGCTACATCCCATTC+CGG | 0.483459 | 5.3:+45471214 | None:intergenic |
| ATGAGAATTCTCGAACGATC+TGG | 0.483710 | 5.3:-45471491 | MS.gene96657:CDS |
| GCTTGTTATCTTCTCATCCA+TGG | 0.488002 | 5.3:-45471384 | MS.gene96657:CDS |
| AGTAATCGGCGGTTTGAAGC+AGG | 0.491235 | 5.3:+45471592 | None:intergenic |
| AAGAGTAATTGTTCTGAAGC+AGG | 0.493059 | 5.3:+45470816 | None:intergenic |
| ATCAAGAGCTTCAACCTCTC+CGG | 0.495473 | 5.3:-45471233 | MS.gene96657:CDS |
| GAGCTTCAACCTCTCCGGAA+TGG | 0.499688 | 5.3:-45471228 | MS.gene96657:CDS |
| TATGTCCTTAGGTTTGAGTC+CGG | 0.505098 | 5.3:+45471343 | None:intergenic |
| AGACACCTGTTAATGGACCT+TGG | 0.510300 | 5.3:-45470458 | MS.gene96657:CDS |
| AAGATCGCGGGCTAAATCAA+TGG | 0.510668 | 5.3:+45471184 | None:intergenic |
| TCCGGAATGGGATGTAGCGC+GGG | 0.514072 | 5.3:-45471215 | MS.gene96657:CDS |
| AAGGATCTTATGGCTATAGC+TGG | 0.515207 | 5.3:-45470885 | MS.gene96657:CDS |
| AAAGATTGAGTATTTCAAAA+GGG | 0.517896 | 5.3:+45471729 | None:intergenic |
| CTTTAACATGAAGTATATTG+TGG | 0.524324 | 5.3:+45471643 | None:intergenic |
| CTCCGGAATGGGATGTAGCG+CGG | 0.535668 | 5.3:-45471216 | MS.gene96657:CDS |
| TTTCGCAAACAAGGTATCCA+TGG | 0.546419 | 5.3:+45471367 | None:intergenic |
| AAGAAGGAGTTGGAGAAAAG+AGG | 0.546500 | 5.3:+45471297 | None:intergenic |
| ATAGCAGACAAAGAAGGAGT+TGG | 0.559010 | 5.3:+45471287 | None:intergenic |
| CATCCCACCTAAACCAACAA+TGG | 0.566152 | 5.3:-45471429 | MS.gene96657:CDS |
| TCAAAAGGAAGGATGAAGAG+AGG | 0.574451 | 5.3:-45470558 | MS.gene96657:CDS |
| ATTACTCCAAACTATTACCA+AGG | 0.582645 | 5.3:-45471113 | MS.gene96657:CDS |
| TTATGAGAAAACAGGAGCAA+AGG | 0.582903 | 5.3:+45471675 | None:intergenic |
| GTTCCATGAAAGTAGCGAAA+GGG | 0.584347 | 5.3:+45471561 | None:intergenic |
| TTGTATGTGATCAAATTGAA+GGG | 0.587629 | 5.3:+45471700 | None:intergenic |
| TGAAAGTAGCGAAAGGGACG+CGG | 0.588590 | 5.3:+45471567 | None:intergenic |
| TTCGAAGAAGAAGACAGAGA+AGG | 0.591426 | 5.3:-45470930 | MS.gene96657:CDS |
| ACTATATTGAATCAAAAGGA+AGG | 0.597855 | 5.3:-45470569 | MS.gene96657:CDS |
| CTTCTGTAACTCATCGATCA+CGG | 0.605553 | 5.3:+45470686 | None:intergenic |
| ATCACCATAGCAGACAAAGA+AGG | 0.610156 | 5.3:+45471281 | None:intergenic |
| ATAAACCTGAAGAAGATCGC+GGG | 0.612944 | 5.3:+45471172 | None:intergenic |
| AACTGTTTATTCAGAATGGG+TGG | 0.613269 | 5.3:-45471065 | MS.gene96657:CDS |
| GGATGAGAAGATAACAAGCT+CGG | 0.613593 | 5.3:+45471388 | None:intergenic |
| TGTTCCATGAAAGTAGCGAA+AGG | 0.619184 | 5.3:+45471560 | None:intergenic |
| CTTCTTCTTCGAAAACGCAA+CGG | 0.633833 | 5.3:+45470940 | None:intergenic |
| GAAAACCGGACTCAAACCTA+AGG | 0.634039 | 5.3:-45471348 | MS.gene96657:CDS |
| TGAAGCAGGCATAATCGACT+AGG | 0.636758 | 5.3:+45471606 | None:intergenic |
| TCTGAAGCAGGTAGAACAAG+AGG | 0.638656 | 5.3:+45470828 | None:intergenic |
| CGATCAATGCAATCTTCCCA+AGG | 0.639317 | 5.3:+45470441 | None:intergenic |
| GCTATCTGCTGAACATGTCG+AGG | 0.645995 | 5.3:-45470655 | MS.gene96657:CDS |
| CGCTACATCCCATTCCGGAG+AGG | 0.648049 | 5.3:+45471219 | None:intergenic |
| CAAGTTGTTAGAACTCACAA+AGG | 0.665486 | 5.3:-45470981 | MS.gene96657:CDS |
| GATAAACCTGAAGAAGATCG+CGG | 0.670461 | 5.3:+45471171 | None:intergenic |
| TCGACTAGGTAGATTGCGCG+TGG | 0.676158 | 5.3:+45471620 | None:intergenic |
| GTTGTTAGAACTCACAAAGG+AGG | 0.692073 | 5.3:-45470978 | MS.gene96657:CDS |
| GGGATGTAATGGATTGCTGG+TGG | 0.704587 | 5.3:+45471446 | None:intergenic |
| TTAAATGCAATAGTGCTGTG+TGG | 0.711523 | 5.3:-45470500 | MS.gene96657:CDS |
| GGGACGCGGCAAGTAATCGG+CGG | 0.714953 | 5.3:+45471581 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCAAATATAACAACTAT+TGG | - | chr5.3:45471316-45471335 | MS.gene96657:CDS | 15.0% |
| !! | AAAAGATTGAGTATTTCAAA+AGG | + | chr5.3:45470443-45470462 | None:intergenic | 20.0% |
| !! | AAAGATTGAGTATTTCAAAA+GGG | + | chr5.3:45470442-45470461 | None:intergenic | 20.0% |
| !! | TTGAACTATATTGAATCAAA+AGG | - | chr5.3:45471595-45471614 | MS.gene96657:CDS | 20.0% |
| ! | ACTATATTGAATCAAAAGGA+AGG | - | chr5.3:45471599-45471618 | MS.gene96657:CDS | 25.0% |
| ! | CTTTAACATGAAGTATATTG+TGG | + | chr5.3:45470528-45470547 | None:intergenic | 25.0% |
| ! | TATGAAGATTATGAGAAAAC+AGG | + | chr5.3:45470504-45470523 | None:intergenic | 25.0% |
| ! | TTGTATGTGATCAAATTGAA+GGG | + | chr5.3:45470471-45470490 | None:intergenic | 25.0% |
| ! | TTTGTATGTGATCAAATTGA+AGG | + | chr5.3:45470472-45470491 | None:intergenic | 25.0% |
| !! | TGTTTGAAATCAGGAATATA+AGG | + | chr5.3:45471424-45471443 | None:intergenic | 25.0% |
| ACAATTTCAGGAATATGAAC+AGG | + | chr5.3:45471757-45471776 | None:intergenic | 30.0% | |
| ACACATCTTCTTCATCTTTA+TGG | - | chr5.3:45471566-45471585 | MS.gene96657:CDS | 30.0% | |
| ACCAAACTGTTTATTCAGAA+TGG | - | chr5.3:45471099-45471118 | MS.gene96657:CDS | 30.0% | |
| AGTTGGAATTTCACTTCAAA+AGG | - | chr5.3:45471264-45471283 | MS.gene96657:CDS | 30.0% | |
| ATAAGAATGTCTATGTCCTT+AGG | + | chr5.3:45470839-45470858 | None:intergenic | 30.0% | |
| ATTACTCCAAACTATTACCA+AGG | - | chr5.3:45471055-45471074 | MS.gene96657:CDS | 30.0% | |
| CCAAACTGTTTATTCAGAAT+GGG | - | chr5.3:45471100-45471119 | MS.gene96657:CDS | 30.0% | |
| TTCACTTCAAAAGGATCTTA+TGG | - | chr5.3:45471273-45471292 | MS.gene96657:CDS | 30.0% | |
| TTCATAGAACTACTACGTTT+AGG | - | chr5.3:45470416-45470435 | MS.gene96657:CDS | 30.0% | |
| ! | ATTTTTGTATTCATGCTGGT+GGG | - | chr5.3:45471455-45471474 | MS.gene96657:CDS | 30.0% |
| ! | GGAATATAAGGCTTCATTTT+AGG | + | chr5.3:45471412-45471431 | None:intergenic | 30.0% |
| !! | TCTTTTCCTTGGTAATAGTT+TGG | + | chr5.3:45471064-45471083 | None:intergenic | 30.0% |
| AACTGTTTATTCAGAATGGG+TGG | - | chr5.3:45471103-45471122 | MS.gene96657:CDS | 35.0% | |
| ACAACAACTGCGTTTGAATT+CGG | + | chr5.3:45471022-45471041 | None:intergenic | 35.0% | |
| AGCATTAAGACACCTGTTAA+TGG | - | chr5.3:45471703-45471722 | MS.gene96657:CDS | 35.0% | |
| ATCGTTCGAGAATTCTCATT+TGG | + | chr5.3:45470676-45470695 | None:intergenic | 35.0% | |
| CAAGTTGTTAGAACTCACAA+AGG | - | chr5.3:45471187-45471206 | MS.gene96657:CDS | 35.0% | |
| CCCATTCTGAATAAACAGTT+TGG | + | chr5.3:45471103-45471122 | None:intergenic | 35.0% | |
| TTAAATGCAATAGTGCTGTG+TGG | - | chr5.3:45471668-45471687 | MS.gene96657:CDS | 35.0% | |
| TTATGAGAAAACAGGAGCAA+AGG | + | chr5.3:45470496-45470515 | None:intergenic | 35.0% | |
| ! | AGAGTATGGCAAATAGCTTT+TGG | - | chr5.3:45471637-45471656 | MS.gene96657:CDS | 35.0% |
| ! | CATTTTTGTATTCATGCTGG+TGG | - | chr5.3:45471454-45471473 | MS.gene96657:CDS | 35.0% |
| ! | CTCTTTCTTTTCACACTCAT+TGG | - | chr5.3:45471370-45471389 | MS.gene96657:CDS | 35.0% |
| ! | GAGCATTTTTGTATTCATGC+TGG | - | chr5.3:45471451-45471470 | MS.gene96657:CDS | 35.0% |
| ! | TCAAACGCTTGTTTGAAATC+AGG | + | chr5.3:45471433-45471452 | None:intergenic | 35.0% |
| !! | AAGAGTAATTGTTCTGAAGC+AGG | + | chr5.3:45471355-45471374 | None:intergenic | 35.0% |
| !! | ATTTGGAACTCAACGCTTTT+CGG | + | chr5.3:45470659-45470678 | None:intergenic | 35.0% |
| AACTCCTTCTTTGTCTGCTA+TGG | - | chr5.3:45470883-45470902 | MS.gene96657:CDS | 40.0% | |
| AAGAAGGAGTTGGAGAAAAG+AGG | + | chr5.3:45470874-45470893 | None:intergenic | 40.0% | |
| AAGGATCTTATGGCTATAGC+TGG | - | chr5.3:45471283-45471302 | MS.gene96657:CDS | 40.0% | |
| ATAAACCTGAAGAAGATCGC+GGG | + | chr5.3:45470999-45471018 | None:intergenic | 40.0% | |
| ATAGCAGACAAAGAAGGAGT+TGG | + | chr5.3:45470884-45470903 | None:intergenic | 40.0% | |
| ATCACCATAGCAGACAAAGA+AGG | + | chr5.3:45470890-45470909 | None:intergenic | 40.0% | |
| ATGAGAATTCTCGAACGATC+TGG | - | chr5.3:45470677-45470696 | MS.gene96657:CDS | 40.0% | |
| CTTCTGTAACTCATCGATCA+CGG | + | chr5.3:45471485-45471504 | None:intergenic | 40.0% | |
| CTTCTTCTTCGAAAACGCAA+CGG | + | chr5.3:45471231-45471250 | None:intergenic | 40.0% | |
| GAAGACAGAGAAGGTAAAGT+TGG | - | chr5.3:45471247-45471266 | MS.gene96657:CDS | 40.0% | |
| GATAAACCTGAAGAAGATCG+CGG | + | chr5.3:45471000-45471019 | None:intergenic | 40.0% | |
| GGATGAGAAGATAACAAGCT+CGG | + | chr5.3:45470783-45470802 | None:intergenic | 40.0% | |
| GTTCCATGAAAGTAGCGAAA+GGG | + | chr5.3:45470610-45470629 | None:intergenic | 40.0% | |
| GTTGTTAGAACTCACAAAGG+AGG | - | chr5.3:45471190-45471209 | MS.gene96657:CDS | 40.0% | |
| TATGTCCTTAGGTTTGAGTC+CGG | + | chr5.3:45470828-45470847 | None:intergenic | 40.0% | |
| TCAAAAGGAAGGATGAAGAG+AGG | - | chr5.3:45471610-45471629 | MS.gene96657:CDS | 40.0% | |
| TGAAGAGAGGTGATAGAGTA+TGG | - | chr5.3:45471623-45471642 | MS.gene96657:CDS | 40.0% | |
| TGTTCCATGAAAGTAGCGAA+AGG | + | chr5.3:45470611-45470630 | None:intergenic | 40.0% | |
| TTCGAAGAAGAAGACAGAGA+AGG | - | chr5.3:45471238-45471257 | MS.gene96657:CDS | 40.0% | |
| TTGGTTTAGGTGGGATGTAA+TGG | + | chr5.3:45470736-45470755 | None:intergenic | 40.0% | |
| ! | GATACCTTGTTTGCGAAAAC+CGG | - | chr5.3:45470806-45470825 | MS.gene96657:CDS | 40.0% |
| ! | GCATTGCTCTTTCTTTTCCT+TGG | + | chr5.3:45471075-45471094 | None:intergenic | 40.0% |
| ! | TTTCGCAAACAAGGTATCCA+TGG | + | chr5.3:45470804-45470823 | None:intergenic | 40.0% |
| !! | CTTCCATTGTTGGTTTAGGT+GGG | + | chr5.3:45470745-45470764 | None:intergenic | 40.0% |
| !! | GCTTCTTCCATTGTTGGTTT+AGG | + | chr5.3:45470749-45470768 | None:intergenic | 40.0% |
| !! | GCTTGTTATCTTCTCATCCA+TGG | - | chr5.3:45470784-45470803 | MS.gene96657:CDS | 40.0% |
| !! | TCTTCCATTGTTGGTTTAGG+TGG | + | chr5.3:45470746-45470765 | None:intergenic | 40.0% |
| !! | TGGCAAATAGCTTTTGGAAG+TGG | - | chr5.3:45471643-45471662 | MS.gene96657:CDS | 40.0% |
| !! | TTTGGAACTCAACGCTTTTC+GGG | + | chr5.3:45470658-45470677 | None:intergenic | 40.0% |
| ATCAAGAGCTTCAACCTCTC+CGG | - | chr5.3:45470935-45470954 | MS.gene96657:CDS | 45.0% | |
| ATTCTCGAACGATCTGGTCT+TGG | - | chr5.3:45470683-45470702 | MS.gene96657:CDS | 45.0% | |
| CATCCCACCTAAACCAACAA+TGG | - | chr5.3:45470739-45470758 | MS.gene96657:CDS | 45.0% | |
| CGATCAATGCAATCTTCCCA+AGG | + | chr5.3:45471730-45471749 | None:intergenic | 45.0% | |
| CGTCTTGCTTCTTCCATTGT+TGG | + | chr5.3:45470755-45470774 | None:intergenic | 45.0% | |
| GAAAACCGGACTCAAACCTA+AGG | - | chr5.3:45470820-45470839 | MS.gene96657:CDS | 45.0% | |
| TCTTCCCAAGGTCCATTAAC+AGG | + | chr5.3:45471718-45471737 | None:intergenic | 45.0% | |
| TGAAGCAGGCATAATCGACT+AGG | + | chr5.3:45470565-45470584 | None:intergenic | 45.0% | |
| ! | AAGATCGCGGGCTAAATCAA+TGG | + | chr5.3:45470987-45471006 | None:intergenic | 45.0% |
| ! | AGACACCTGTTAATGGACCT+TGG | - | chr5.3:45471710-45471729 | MS.gene96657:CDS | 45.0% |
| ! | GACACCTGTTAATGGACCTT+GGG | - | chr5.3:45471711-45471730 | MS.gene96657:CDS | 45.0% |
| ! | TCTGAAGCAGGTAGAACAAG+AGG | + | chr5.3:45471343-45471362 | None:intergenic | 45.0% |
| !! | AGAATGACGCTGCATCGATT+CGG | - | chr5.3:45471541-45471560 | MS.gene96657:CDS | 45.0% |
| AAAGGGACGCGGCAAGTAAT+CGG | + | chr5.3:45470593-45470612 | None:intergenic | 50.0% | |
| AGCTTCAACCTCTCCGGAAT+GGG | - | chr5.3:45470941-45470960 | MS.gene96657:CDS | 50.0% | |
| GCTATCTGCTGAACATGTCG+AGG | - | chr5.3:45471513-45471532 | MS.gene96657:CDS | 50.0% | |
| TGAAAGTAGCGAAAGGGACG+CGG | + | chr5.3:45470604-45470623 | None:intergenic | 50.0% | |
| TTTAGCCCGCGATCTTCTTC+AGG | - | chr5.3:45470991-45471010 | MS.gene96657:CDS | 50.0% | |
| ! | AGTAATCGGCGGTTTGAAGC+AGG | + | chr5.3:45470579-45470598 | None:intergenic | 50.0% |
| ! | CGTCCCTTTCGCTACTTTCA+TGG | - | chr5.3:45470604-45470623 | MS.gene96657:CDS | 50.0% |
| ! | GAGTCCGGTTTTCGCAAACA+AGG | + | chr5.3:45470813-45470832 | None:intergenic | 50.0% |
| !! | GAATGACGCTGCATCGATTC+GGG | - | chr5.3:45471542-45471561 | MS.gene96657:CDS | 50.0% |
| !! | GGGATGTAATGGATTGCTGG+TGG | + | chr5.3:45470725-45470744 | None:intergenic | 50.0% |
| !! | GGTGGGATGTAATGGATTGC+TGG | + | chr5.3:45470728-45470747 | None:intergenic | 50.0% |
| !!! | CGCTTTTCGGGTTGTTCTTG+AGG | + | chr5.3:45470646-45470665 | None:intergenic | 50.0% |
| GAGCTTCAACCTCTCCGGAA+TGG | - | chr5.3:45470940-45470959 | MS.gene96657:CDS | 55.0% | |
| TCGACTAGGTAGATTGCGCG+TGG | + | chr5.3:45470551-45470570 | None:intergenic | 55.0% | |
| ACCCGCGCTACATCCCATTC+CGG | + | chr5.3:45470957-45470976 | None:intergenic | 60.0% | |
| CGCTACATCCCATTCCGGAG+AGG | + | chr5.3:45470952-45470971 | None:intergenic | 60.0% | |
| CTCCGGAATGGGATGTAGCG+CGG | - | chr5.3:45470952-45470971 | MS.gene96657:CDS | 60.0% | |
| TCCGGAATGGGATGTAGCGC+GGG | - | chr5.3:45470953-45470972 | MS.gene96657:CDS | 60.0% | |
| GGGACGCGGCAAGTAATCGG+CGG | + | chr5.3:45470590-45470609 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 45470404 | 45471786 | 45470404 | ID=MS.gene96657 |
| chr5.3 | mRNA | 45470404 | 45471786 | 45470404 | ID=MS.gene96657.t1;Parent=MS.gene96657 |
| chr5.3 | exon | 45470404 | 45471786 | 45470404 | ID=MS.gene96657.t1.exon1;Parent=MS.gene96657.t1 |
| chr5.3 | CDS | 45470404 | 45471786 | 45470404 | ID=cds.MS.gene96657.t1;Parent=MS.gene96657.t1 |
| Gene Sequence |
| Protein sequence |
