AlfalfaGEDB | MS.gene98179

M. sativa cultivar XinJiangDaYe / MS.gene98179

KEGG: K03236
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005488 GO:0006413 GO:0008135 GO:0008150 GO:0008152 GO:0009987 GO:0044237 GO:0045182 GO:0090079 GO:0097159 GO:1901363
PFAM: PF01176.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98179.t1	XP_013444714.1	98.6	146	2	0	1	146	1	146	1.30E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98179.t1	P56331	90.5	147	11	2	1	146	1	145	6.7e-67	254.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98179.t1	G7ZYQ6	98.6	146	2	0	1	146	1	146	9.6e-75	288.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04920	MS.gene98179	0.80594	1.05E-49	-1.69E-46
MS.gene049254	MS.gene98179	0.82236	2.47E-53	-1.69E-46
MS.gene049283	MS.gene98179	0.814806	1.28E-51	-1.69E-46
MS.gene049343	MS.gene98179	0.80662	7.53E-50	-1.69E-46
MS.gene049419	MS.gene98179	-0.821425	4.06E-53	-1.69E-46
MS.gene049499	MS.gene98179	0.833661	4.69E-56	-1.69E-46
MS.gene049983	MS.gene98179	0.81237	4.39E-51	-1.69E-46
MS.gene050026	MS.gene98179	0.811478	6.86E-51	-1.69E-46
MS.gene050559	MS.gene98179	0.805447	1.33E-49	-1.69E-46
MS.gene050561	MS.gene98179	0.801158	1.03E-48	-1.69E-46
MS.gene051181	MS.gene98179	0.807711	4.42E-50	-1.69E-46
MS.gene051332	MS.gene98179	0.804565	2.03E-49	-1.69E-46
MS.gene051699	MS.gene98179	0.808248	3.40E-50	-1.69E-46
MS.gene052108	MS.gene98179	0.820084	8.27E-53	-1.69E-46
MS.gene052518	MS.gene98179	0.830321	3.14E-55	-1.69E-46
MS.gene052519	MS.gene98179	0.842477	2.53E-58	-1.69E-46
MS.gene052582	MS.gene98179	0.80569	1.18E-49	-1.69E-46
MS.gene052584	MS.gene98179	0.80539	1.37E-49	-1.69E-46
MS.gene052685	MS.gene98179	0.816245	6.11E-52	-1.69E-46
MS.gene052954	MS.gene98179	0.811653	6.29E-51	-1.69E-46
MS.gene053006	MS.gene98179	0.812648	3.81E-51	-1.69E-46
MS.gene053272	MS.gene98179	-0.828739	7.60E-55	-1.69E-46
MS.gene053298	MS.gene98179	0.8069	6.57E-50	-1.69E-46
MS.gene05350	MS.gene98179	0.800882	1.17E-48	-1.69E-46
MS.gene053963	MS.gene98179	0.835228	1.90E-56	-1.69E-46
MS.gene05415	MS.gene98179	0.810403	1.17E-50	-1.69E-46
MS.gene054222	MS.gene98179	0.801926	7.15E-49	-1.69E-46
MS.gene05423	MS.gene98179	0.815506	8.93E-52	-1.69E-46
MS.gene054479	MS.gene98179	0.833735	4.50E-56	-1.69E-46
MS.gene054651	MS.gene98179	0.826432	2.72E-54	-1.69E-46
MS.gene055228	MS.gene98179	0.811117	8.22E-51	-1.69E-46
MS.gene055724	MS.gene98179	0.801945	7.08E-49	-1.69E-46
MS.gene055818	MS.gene98179	0.822507	2.28E-53	-1.69E-46
MS.gene055841	MS.gene98179	0.805121	1.56E-49	-1.69E-46
MS.gene056174	MS.gene98179	0.807032	6.16E-50	-1.69E-46
MS.gene056272	MS.gene98179	0.807452	5.02E-50	-1.69E-46
MS.gene056699	MS.gene98179	0.810384	1.18E-50	-1.69E-46
MS.gene056776	MS.gene98179	0.811373	7.23E-51	-1.69E-46
MS.gene05686	MS.gene98179	0.802283	6.04E-49	-1.69E-46
MS.gene05690	MS.gene98179	0.813812	2.12E-51	-1.69E-46
MS.gene057102	MS.gene98179	0.823453	1.37E-53	-1.69E-46
MS.gene057208	MS.gene98179	0.862414	5.15E-64	-1.69E-46
MS.gene057598	MS.gene98179	0.809583	1.76E-50	-1.69E-46
MS.gene058008	MS.gene98179	0.828997	6.58E-55	-1.69E-46
MS.gene058009	MS.gene98179	0.82493	6.18E-54	-1.69E-46
MS.gene058037	MS.gene98179	0.819907	9.08E-53	-1.69E-46
MS.gene058159	MS.gene98179	0.822133	2.79E-53	-1.69E-46
MS.gene058279	MS.gene98179	0.823968	1.04E-53	-1.69E-46
MS.gene058294	MS.gene98179	0.80207	6.68E-49	-1.69E-46
MS.gene058592	MS.gene98179	0.808898	2.47E-50	-1.69E-46
MS.gene058781	MS.gene98179	0.803586	3.25E-49	-1.69E-46
MS.gene058783	MS.gene98179	0.826132	3.21E-54	-1.69E-46
MS.gene058979	MS.gene98179	0.818491	1.91E-52	-1.69E-46
MS.gene05952	MS.gene98179	0.84065	7.65E-58	-1.69E-46
MS.gene05985	MS.gene98179	-0.824779	6.71E-54	-1.69E-46
MS.gene059935	MS.gene98179	0.804406	2.19E-49	-1.69E-46
MS.gene060257	MS.gene98179	0.80944	1.89E-50	-1.69E-46
MS.gene060259	MS.gene98179	0.81707	3.99E-52	-1.69E-46
MS.gene060365	MS.gene98179	0.804222	2.40E-49	-1.69E-46
MS.gene060521	MS.gene98179	0.801864	7.36E-49	-1.69E-46
MS.gene060777	MS.gene98179	0.81908	1.40E-52	-1.69E-46
MS.gene060879	MS.gene98179	0.800444	1.44E-48	-1.69E-46
MS.gene060932	MS.gene98179	0.811557	6.60E-51	-1.69E-46
MS.gene060935	MS.gene98179	0.823587	1.28E-53	-1.69E-46
MS.gene060937	MS.gene98179	0.825148	5.49E-54	-1.69E-46
MS.gene061100	MS.gene98179	0.810393	1.18E-50	-1.69E-46
MS.gene061160	MS.gene98179	0.801896	7.25E-49	-1.69E-46
MS.gene061188	MS.gene98179	0.817293	3.55E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98179.t1	MTR_8g028180	98.630	146	2	0	1	146	1	146	3.24e-101	286
MS.gene98179.t1	MTR_3g005520	83.333	150	21	1	1	146	1	150	2.32e-77	226

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98179.t1	AT2G04520	87.671	146	17	1	1	146	1	145	8.27e-85	245
MS.gene98179.t1	AT5G35680	86.986	146	18	1	1	146	1	145	7.68e-80	232
MS.gene98179.t1	AT5G35680	86.986	146	18	1	1	146	1	145	7.68e-80	232
MS.gene98179.t1	AT5G35680	93.277	119	8	0	1	119	1	119	6.39e-78	228

Find 48 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGGGTTGCGTGATTATC+AGG	0.308178	8.2:+68985833	MS.gene98179:CDS
CCTCCCTTTCCCTTGTTCTT+CGG	0.331257	8.2:-68985603	None:intergenic
GCTGGGGATATTATTCTTGT+TGG	0.331915	8.2:+68985816	MS.gene98179:CDS
TCATTAAGCCTCACACCTTC+CGG	0.357600	8.2:-68985927	None:intergenic
GGAATTGGTGGTGGGATTGA+TGG	0.360212	8.2:+68985951	MS.gene98179:CDS
CTGGGGATATTATTCTTGTT+GGG	0.363705	8.2:+68985817	MS.gene98179:CDS
GAAAGATGCACAAGAAGGTT+TGG	0.408452	8.2:+68985787	MS.gene98179:CDS
CTTTCCCTTGTTCTTCGGCA+TGG	0.425552	8.2:-68985598	None:intergenic
GAAGATATTGATAAGATTTG+AGG	0.433764	8.2:+68986020	MS.gene98179:CDS
GAATTGGTGGTGGGATTGAT+GGG	0.434528	8.2:+68985952	MS.gene98179:CDS
GCACAGGTTCTTCGAATGCT+TGG	0.438659	8.2:+68985705	MS.gene98179:CDS
GAAGGCGTATAGCGAGCTTC+CGG	0.446342	8.2:+68985908	MS.gene98179:CDS
AAGAAGGTTTGGATTGCTGC+TGG	0.450047	8.2:+68985798	MS.gene98179:CDS
TTCAACAACCTCGCCTCATC+CGG	0.452149	8.2:-68985888	None:intergenic
GGGGATGATGATGAGGGTGA+TGG	0.471229	8.2:+68985972	MS.gene98179:CDS
GGGATGATGATGAGGGTGAT+GGG	0.477444	8.2:+68985973	MS.gene98179:CDS
AAGATATTGATAAGATTTGA+GGG	0.478420	8.2:+68986021	MS.gene98179:CDS
GAATGATTATATTGAGTTTG+AGG	0.491210	8.2:+68985995	MS.gene98179:CDS
GTGAGGCTTAATGAAGGAAT+TGG	0.513375	8.2:+68985936	MS.gene98179:CDS
CTTAATGAAGGAATTGGTGG+TGG	0.513624	8.2:+68985942	MS.gene98179:CDS
GAAGGTTTGGATTGCTGCTG+GGG	0.517248	8.2:+68985800	MS.gene98179:CDS
GCGTGATTATCAGGATGATA+AGG	0.518535	8.2:+68985842	MS.gene98179:CDS
GGATGAGGCGAGGTTGTTGA+AGG	0.519284	8.2:+68985890	MS.gene98179:CDS
AGAAGGTTTGGATTGCTGCT+GGG	0.519846	8.2:+68985799	MS.gene98179:CDS
CCTTCTTGTGCATCTTTCCA+CGG	0.526928	8.2:-68985782	None:intergenic
ATGCCGAAGAACAAGGGAAA+GGG	0.538255	8.2:+68985600	MS.gene98179:CDS
GTTCTTCGAATGCTTGGAAA+CGG	0.544262	8.2:+68985711	MS.gene98179:CDS
GATTGATGGGGATGATGATG+AGG	0.545936	8.2:+68985965	MS.gene98179:CDS
TTCAACCATGCCGAAGAACA+AGG	0.546102	8.2:+68985593	None:intergenic
CTTGAAGTATATGCCGGATG+AGG	0.557673	8.2:+68985875	MS.gene98179:CDS
ATTGATGGGGATGATGATGA+GGG	0.568262	8.2:+68985966	MS.gene98179:CDS
CTTTGTTCCATCGATACACA+TGG	0.573074	8.2:-68985745	None:intergenic
GGAGGAAAGAATCGCAAGAG+AGG	0.578624	8.2:+68985621	MS.gene98179:CDS
CCGTGGAAAGATGCACAAGA+AGG	0.600822	8.2:+68985782	MS.gene98179:CDS
CATGCCGAAGAACAAGGGAA+AGG	0.602323	8.2:+68985599	None:intergenic
TTAATGAAGGAATTGGTGGT+GGG	0.613970	8.2:+68985943	MS.gene98179:CDS
AAGCGTCTCTGTCATATCCG+TGG	0.616715	8.2:+68985765	MS.gene98179:CDS
GCGAGCTTCCGGAAGGTGTG+AGG	0.621721	8.2:+68985919	MS.gene98179:CDS
GAAGGTGTGAGGCTTAATGA+AGG	0.623962	8.2:+68985930	MS.gene98179:CDS
AGATGGTCAAGAATATGCAC+AGG	0.626844	8.2:+68985689	MS.gene98179:CDS
TGTGAAGCCATGTGTATCGA+TGG	0.627821	8.2:+68985738	MS.gene98179:CDS
CCGAAGAACAAGGGAAAGGG+AGG	0.634335	8.2:+68985603	MS.gene98179:CDS
AATTGGTGGTGGGATTGATG+GGG	0.634571	8.2:+68985953	MS.gene98179:CDS
AGGCTTAATGAAGGAATTGG+TGG	0.634691	8.2:+68985939	MS.gene98179:CDS
TCAACCATGCCGAAGAACAA+GGG	0.635918	8.2:+68985594	None:intergenic
GCGTATAGCGAGCTTCCGGA+AGG	0.641188	8.2:+68985912	MS.gene98179:CDS
TGTGATCTTGAAGTATATGC+CGG	0.651550	8.2:+68985869	MS.gene98179:CDS
AGTATATGCCGGATGAGGCG+AGG	0.665508	8.2:+68985880	MS.gene98179:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAATGATTATATTGAGTTTG+AGG	+	chr8.2:68985995-68986014	MS.gene98179:CDS	25.0%
	CTGGGGATATTATTCTTGTT+GGG	+	chr8.2:68985817-68985836	MS.gene98179:CDS	35.0%
	TGTGATCTTGAAGTATATGC+CGG	+	chr8.2:68985869-68985888	MS.gene98179:CDS	35.0%
!	TTAATGAAGGAATTGGTGGT+GGG	+	chr8.2:68985943-68985962	MS.gene98179:CDS	35.0%
	AGATGGTCAAGAATATGCAC+AGG	+	chr8.2:68985689-68985708	MS.gene98179:CDS	40.0%
	AGGCTTAATGAAGGAATTGG+TGG	+	chr8.2:68985939-68985958	MS.gene98179:CDS	40.0%
	CTTAATGAAGGAATTGGTGG+TGG	+	chr8.2:68985942-68985961	MS.gene98179:CDS	40.0%
	CTTTGTTCCATCGATACACA+TGG	-	chr8.2:68985748-68985767	None:intergenic	40.0%
	GAAAGATGCACAAGAAGGTT+TGG	+	chr8.2:68985787-68985806	MS.gene98179:CDS	40.0%
	GCGTGATTATCAGGATGATA+AGG	+	chr8.2:68985842-68985861	MS.gene98179:CDS	40.0%
	GCTGGGGATATTATTCTTGT+TGG	+	chr8.2:68985816-68985835	MS.gene98179:CDS	40.0%
	GTGAGGCTTAATGAAGGAAT+TGG	+	chr8.2:68985936-68985955	MS.gene98179:CDS	40.0%
	GTTCTTCGAATGCTTGGAAA+CGG	+	chr8.2:68985711-68985730	MS.gene98179:CDS	40.0%
!	ATTGATGGGGATGATGATGA+GGG	+	chr8.2:68985966-68985985	MS.gene98179:CDS	40.0%
!!	AAAGCGTGAGCTTGTTTTCA+AGG	+	chr8.2:68985665-68985684	MS.gene98179:CDS	40.0%
!!	GAGCTTGTTTTCAAGGAAGA+TGG	+	chr8.2:68985672-68985691	MS.gene98179:CDS	40.0%
	AAGAAGGTTTGGATTGCTGC+TGG	+	chr8.2:68985798-68985817	MS.gene98179:CDS	45.0%
	AGAAGGTTTGGATTGCTGCT+GGG	+	chr8.2:68985799-68985818	MS.gene98179:CDS	45.0%
	ATGCCGAAGAACAAGGGAAA+GGG	+	chr8.2:68985600-68985619	MS.gene98179:CDS	45.0%
	CCTTCTTGTGCATCTTTCCA+CGG	-	chr8.2:68985785-68985804	None:intergenic	45.0%
	CTTGAAGTATATGCCGGATG+AGG	+	chr8.2:68985875-68985894	MS.gene98179:CDS	45.0%
	TCATTAAGCCTCACACCTTC+CGG	-	chr8.2:68985930-68985949	None:intergenic	45.0%
	TGTGAAGCCATGTGTATCGA+TGG	+	chr8.2:68985738-68985757	MS.gene98179:CDS	45.0%
!	AAGCTCACGCTTTTCGTCAT+CGG	-	chr8.2:68985658-68985677	None:intergenic	45.0%
!	GAAGGTGTGAGGCTTAATGA+AGG	+	chr8.2:68985930-68985949	MS.gene98179:CDS	45.0%
!	GATTGATGGGGATGATGATG+AGG	+	chr8.2:68985965-68985984	MS.gene98179:CDS	45.0%
!	TGTTGGGTTGCGTGATTATC+AGG	+	chr8.2:68985833-68985852	MS.gene98179:CDS	45.0%
!!	AATTGGTGGTGGGATTGATG+GGG	+	chr8.2:68985953-68985972	MS.gene98179:CDS	45.0%
!!	GAATTGGTGGTGGGATTGAT+GGG	+	chr8.2:68985952-68985971	MS.gene98179:CDS	45.0%
	AAGCGTCTCTGTCATATCCG+TGG	+	chr8.2:68985765-68985784	MS.gene98179:CDS	50.0%
	CCGTGGAAAGATGCACAAGA+AGG	+	chr8.2:68985782-68985801	MS.gene98179:CDS	50.0%
	CCTCCCTTTCCCTTGTTCTT+CGG	-	chr8.2:68985606-68985625	None:intergenic	50.0%
	GAAGGTTTGGATTGCTGCTG+GGG	+	chr8.2:68985800-68985819	MS.gene98179:CDS	50.0%
	GCACAGGTTCTTCGAATGCT+TGG	+	chr8.2:68985705-68985724	MS.gene98179:CDS	50.0%
	GGAGGAAAGAATCGCAAGAG+AGG	+	chr8.2:68985621-68985640	MS.gene98179:CDS	50.0%
	TTCAACAACCTCGCCTCATC+CGG	-	chr8.2:68985891-68985910	None:intergenic	50.0%
!	GGGATGATGATGAGGGTGAT+GGG	+	chr8.2:68985973-68985992	MS.gene98179:CDS	50.0%
!!	GGAATTGGTGGTGGGATTGA+TGG	+	chr8.2:68985951-68985970	MS.gene98179:CDS	50.0%
	AGTATATGCCGGATGAGGCG+AGG	+	chr8.2:68985880-68985899	MS.gene98179:CDS	55.0%
	CCGAAGAACAAGGGAAAGGG+AGG	+	chr8.2:68985603-68985622	MS.gene98179:CDS	55.0%
	GGATGAGGCGAGGTTGTTGA+AGG	+	chr8.2:68985890-68985909	MS.gene98179:CDS	55.0%
!	GAAGGCGTATAGCGAGCTTC+CGG	+	chr8.2:68985908-68985927	MS.gene98179:CDS	55.0%
!	GGGGATGATGATGAGGGTGA+TGG	+	chr8.2:68985972-68985991	MS.gene98179:CDS	55.0%
	GCGTATAGCGAGCTTCCGGA+AGG	+	chr8.2:68985912-68985931	MS.gene98179:CDS	60.0%
!	GCGAGCTTCCGGAAGGTGTG+AGG	+	chr8.2:68985919-68985938	MS.gene98179:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	68985600	68986040	68985600	ID=MS.gene98179
chr8.2	mRNA	68985600	68986040	68985600	ID=MS.gene98179.t1;Parent=MS.gene98179
chr8.2	exon	68985600	68986040	68985600	ID=MS.gene98179.t1.exon1;Parent=MS.gene98179.t1
chr8.2	CDS	68985600	68986040	68985600	ID=cds.MS.gene98179.t1;Parent=MS.gene98179.t1

Gene Sequence

>MS.gene98179

ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAGAGAGGAAAGAACGAAGCCGATGACGAAAAGCGTGAGCTTGTTTTCAAGGAAGATGGTCAAGAATATGCACAGGTTCTTCGAATGCTTGGAAACGGTCGTTGTGAAGCCATGTGTATCGATGGAACAAAGCGTCTCTGTCATATCCGTGGAAAGATGCACAAGAAGGTTTGGATTGCTGCTGGGGATATTATTCTTGTTGGGTTGCGTGATTATCAGGATGATAAGGCTGATGTGATCTTGAAGTATATGCCGGATGAGGCGAGGTTGTTGAAGGCGTATAGCGAGCTTCCGGAAGGTGTGAGGCTTAATGAAGGAATTGGTGGTGGGATTGATGGGGATGATGATGAGGGTGATGGGAATGATTATATTGAGTTTGAGGATGAAGATATTGATAAGATTTGA

Protein sequence

>MS.gene98179.t1

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYSELPEGVRLNEGIGGGIDGDDDEGDGNDYIEFEDEDIDKI