Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99465.t1 | XP_003604820.1 | 97.5 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 8.20E-91 | 343.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99465.t1 | G7JRA4 | 97.5 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 5.9e-91 | 343.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene99465 | 0.810265 | 1.26E-50 | -1.69E-46 |
| MS.gene049293 | MS.gene99465 | -0.827698 | 1.35E-54 | -1.69E-46 |
| MS.gene049378 | MS.gene99465 | 0.801629 | 8.23E-49 | -1.69E-46 |
| MS.gene049424 | MS.gene99465 | 0.817912 | 2.58E-52 | -1.69E-46 |
| MS.gene049653 | MS.gene99465 | 0.825562 | 4.38E-54 | -1.69E-46 |
| MS.gene04991 | MS.gene99465 | -0.822457 | 2.34E-53 | -1.69E-46 |
| MS.gene050105 | MS.gene99465 | 0.811021 | 8.62E-51 | -1.69E-46 |
| MS.gene050288 | MS.gene99465 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene050317 | MS.gene99465 | 0.808026 | 3.79E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene99465 | -0.809699 | 1.66E-50 | -1.69E-46 |
| MS.gene050987 | MS.gene99465 | 0.812782 | 3.56E-51 | -1.69E-46 |
| MS.gene051122 | MS.gene99465 | 0.821478 | 3.95E-53 | -1.69E-46 |
| MS.gene051245 | MS.gene99465 | 0.809436 | 1.89E-50 | -1.69E-46 |
| MS.gene051655 | MS.gene99465 | 0.803207 | 3.89E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene99465 | 0.80139 | 9.21E-49 | -1.69E-46 |
| MS.gene052824 | MS.gene99465 | 0.80694 | 6.44E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene99465 | 0.83051 | 2.82E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene99465 | 0.807277 | 5.47E-50 | -1.69E-46 |
| MS.gene053340 | MS.gene99465 | 0.800293 | 1.54E-48 | -1.69E-46 |
| MS.gene05344 | MS.gene99465 | -0.812045 | 5.16E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene99465 | 0.816599 | 5.09E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene99465 | 0.81047 | 1.13E-50 | -1.69E-46 |
| MS.gene054536 | MS.gene99465 | 0.805214 | 1.49E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene99465 | 0.800176 | 1.63E-48 | -1.69E-46 |
| MS.gene055222 | MS.gene99465 | 0.804033 | 2.62E-49 | -1.69E-46 |
| MS.gene055442 | MS.gene99465 | 0.800735 | 1.25E-48 | -1.69E-46 |
| MS.gene056480 | MS.gene99465 | 0.81045 | 1.15E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene99465 | 0.816882 | 4.40E-52 | -1.69E-46 |
| MS.gene056849 | MS.gene99465 | 0.826028 | 3.40E-54 | -1.69E-46 |
| MS.gene056975 | MS.gene99465 | 0.825382 | 4.83E-54 | -1.69E-46 |
| MS.gene057079 | MS.gene99465 | 0.807225 | 5.61E-50 | -1.69E-46 |
| MS.gene057108 | MS.gene99465 | 0.813928 | 2.00E-51 | -1.69E-46 |
| MS.gene057109 | MS.gene99465 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene057110 | MS.gene99465 | 0.809152 | 2.18E-50 | -1.69E-46 |
| MS.gene057392 | MS.gene99465 | 0.812711 | 3.70E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene99465 | -0.806958 | 6.39E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene99465 | 0.844712 | 6.38E-59 | -1.69E-46 |
| MS.gene058238 | MS.gene99465 | 0.812658 | 3.80E-51 | -1.69E-46 |
| MS.gene059360 | MS.gene99465 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene059872 | MS.gene99465 | -0.843194 | 1.63E-58 | -1.69E-46 |
| MS.gene060084 | MS.gene99465 | 0.807959 | 3.92E-50 | -1.69E-46 |
| MS.gene060297 | MS.gene99465 | 0.843464 | 1.38E-58 | -1.69E-46 |
| MS.gene060840 | MS.gene99465 | 0.805411 | 1.35E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99465.t1 | MTR_4g019130 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.05e-141 | 393 |
| MS.gene99465.t1 | MTR_4g019150 | 72.816 | 206 | 47 | 3 | 1 | 201 | 1 | 202 | 2.71e-104 | 298 |
| MS.gene99465.t1 | MTR_7g104650 | 37.000 | 200 | 108 | 10 | 11 | 201 | 102 | 292 | 2.24e-24 | 97.4 |
| MS.gene99465.t1 | MTR_2g054130 | 37.500 | 192 | 94 | 6 | 11 | 201 | 211 | 377 | 4.20e-22 | 92.8 |
| MS.gene99465.t1 | MTR_2g055010 | 37.079 | 178 | 86 | 5 | 11 | 187 | 211 | 363 | 2.26e-19 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99465.t1 | AT2G37970 | 59.722 | 216 | 71 | 3 | 1 | 201 | 32 | 246 | 7.43e-88 | 259 |
| MS.gene99465.t1 | AT5G20140 | 34.031 | 191 | 103 | 5 | 11 | 201 | 206 | 373 | 4.93e-21 | 89.7 |
| MS.gene99465.t1 | AT3G10130 | 37.000 | 200 | 108 | 10 | 11 | 201 | 115 | 305 | 1.21e-19 | 85.1 |
| MS.gene99465.t1 | AT5G20140 | 33.898 | 177 | 94 | 5 | 11 | 187 | 206 | 359 | 1.64e-18 | 82.8 |
Find 36 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACAAAGATGGTGGGTTTA+AGG | 0.180691 | 4.2:-83321849 | MS.gene99465:CDS |
| GAGGAAGGTGGGAAGAAATA+TGG | 0.218011 | 4.2:-83321587 | MS.gene99465:CDS |
| TTGTCTCCTTTGAATGTTGA+TGG | 0.274828 | 4.2:+83321866 | None:intergenic |
| AAATATGGGGTGGTGACTTT+TGG | 0.302611 | 4.2:-83321572 | MS.gene99465:CDS |
| TTCTTCTCATTTGTCACAAC+TGG | 0.315475 | 4.2:+83321701 | None:intergenic |
| TTTGATATGGGTTTAGCATT+TGG | 0.339119 | 4.2:-83321992 | None:intergenic |
| AGGAAGGTGGGAAGAAATAT+GGG | 0.347201 | 4.2:-83321586 | MS.gene99465:CDS |
| CGATCACAATTCCAATGTTT+AGG | 0.352816 | 4.2:-83321433 | MS.gene99465:CDS |
| ATCATCATGGTTCATTCAAC+AGG | 0.393338 | 4.2:+83321392 | None:intergenic |
| GGAGAAGTTGAGGTTGTGTT+TGG | 0.422907 | 4.2:-83321513 | MS.gene99465:CDS |
| ATCACAACCCTCTCATCAAT+AGG | 0.466452 | 4.2:+83321614 | None:intergenic |
| TTGTGATAAGAGAGGAAGGT+GGG | 0.471919 | 4.2:-83321598 | MS.gene99465:CDS |
| TGAAGTGGTGAAGGAGAAAG+TGG | 0.481060 | 4.2:-83321534 | MS.gene99465:CDS |
| AGGGTTGTGATAAGAGAGGA+AGG | 0.486753 | 4.2:-83321602 | MS.gene99465:CDS |
| AGGAGAAAGTGGAGAAGTTG+AGG | 0.492065 | 4.2:-83321523 | MS.gene99465:CDS |
| TCCAATGTTTAGGACTAATG+AGG | 0.513944 | 4.2:-83321423 | MS.gene99465:CDS |
| AGGTTGTGTTTGGAGAAAGA+TGG | 0.524733 | 4.2:-83321503 | MS.gene99465:CDS |
| TGAGAGGGTTGTGATAAGAG+AGG | 0.529346 | 4.2:-83321606 | MS.gene99465:CDS |
| GGCAAGTGATGAAGTGGTGA+AGG | 0.533494 | 4.2:-83321543 | MS.gene99465:CDS |
| TATGATCCATCAACATTCAA+AGG | 0.533544 | 4.2:-83321872 | MS.gene99465:CDS |
| AACATTGGAATTGTGATCGC+AGG | 0.545246 | 4.2:+83321437 | None:intergenic |
| ACCTAAGCCTATTGATGAGA+GGG | 0.548573 | 4.2:-83321621 | MS.gene99465:CDS |
| ACCCTCTCATCAATAGGCTT+AGG | 0.549333 | 4.2:+83321620 | None:intergenic |
| ATTGGAATTGTGATCGCAGG+TGG | 0.552002 | 4.2:+83321440 | None:intergenic |
| ACCTCATTAGTCCTAAACAT+TGG | 0.572004 | 4.2:+83321422 | None:intergenic |
| CTGATTTCAGCTACTAGAGA+AGG | 0.573096 | 4.2:+83321896 | None:intergenic |
| TTCAAAGGAGACAAAGATGG+TGG | 0.597630 | 4.2:-83321857 | MS.gene99465:CDS |
| ACATTCAAAGGAGACAAAGA+TGG | 0.611112 | 4.2:-83321860 | MS.gene99465:CDS |
| TACCTAAGCCTATTGATGAG+AGG | 0.615966 | 4.2:-83321622 | MS.gene99465:CDS |
| AGGAGTGGCAAGTGATGAAG+TGG | 0.624838 | 4.2:-83321549 | MS.gene99465:CDS |
| TCAAAGGAGACAAAGATGGT+GGG | 0.635974 | 4.2:-83321856 | MS.gene99465:CDS |
| AGGTGGGAAGAAATATGGGG+TGG | 0.665542 | 4.2:-83321582 | MS.gene99465:CDS |
| GTTGTGATAAGAGAGGAAGG+TGG | 0.671478 | 4.2:-83321599 | MS.gene99465:CDS |
| GACTTCTCCAGTTATCACAA+AGG | 0.674539 | 4.2:-83321762 | MS.gene99465:CDS |
| GGAAGGTGGGAAGAAATATG+GGG | 0.684324 | 4.2:-83321585 | MS.gene99465:CDS |
| GACAAATGAGAAGAACAAGA+TGG | 0.687039 | 4.2:-83321693 | MS.gene99465:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTGAGTATTTCATATTT+TGG | + | chr4.2:83321442-83321461 | None:intergenic | 20.0% |
| ! | TTCTATGTATGAAAAAGTAG+AGG | - | chr4.2:83321741-83321760 | MS.gene99465:CDS | 25.0% |
| !! | GTTGATTACATAGGAATTTT+TGG | - | chr4.2:83321568-83321587 | MS.gene99465:CDS | 25.0% |
| !!! | AAAGATGGTTTTAAGGTTAT+TGG | - | chr4.2:83321901-83321920 | MS.gene99465:CDS | 25.0% |
| !!! | AAGGTTTTAGTTGATTACAT+AGG | - | chr4.2:83321559-83321578 | MS.gene99465:CDS | 25.0% |
| !!! | GTTATTGGAGATTTTTTGTT+GGG | - | chr4.2:83321916-83321935 | MS.gene99465:CDS | 25.0% |
| !!! | TCTACTTTTTCATACATAGA+AGG | + | chr4.2:83321742-83321761 | None:intergenic | 25.0% |
| !!! | TTTTGTGTTTTTGTGTTTTG+TGG | + | chr4.2:83321598-83321617 | None:intergenic | 25.0% |
| TATGATCCATCAACATTCAA+AGG | - | chr4.2:83321517-83321536 | MS.gene99465:CDS | 30.0% | |
| ! | TTGTTTTCCTTTGTGATAAC+TGG | + | chr4.2:83321637-83321656 | None:intergenic | 30.0% |
| !! | TTTGGAGAAAGATGGTTTTA+AGG | - | chr4.2:83321894-83321913 | MS.gene99465:CDS | 30.0% |
| !!! | GGTTATTGGAGATTTTTTGT+TGG | - | chr4.2:83321915-83321934 | MS.gene99465:CDS | 30.0% |
| ACATTCAAAGGAGACAAAGA+TGG | - | chr4.2:83321529-83321548 | MS.gene99465:CDS | 35.0% | |
| ACCTCATTAGTCCTAAACAT+TGG | + | chr4.2:83321970-83321989 | None:intergenic | 35.0% | |
| CGATCACAATTCCAATGTTT+AGG | - | chr4.2:83321956-83321975 | MS.gene99465:CDS | 35.0% | |
| GACAAATGAGAAGAACAAGA+TGG | - | chr4.2:83321696-83321715 | MS.gene99465:CDS | 35.0% | |
| TTCTTCTCATTTGTCACAAC+TGG | + | chr4.2:83321691-83321710 | None:intergenic | 35.0% | |
| TTGTCTCCTTTGAATGTTGA+TGG | + | chr4.2:83321526-83321545 | None:intergenic | 35.0% | |
| ! | TCCAATGTTTAGGACTAATG+AGG | - | chr4.2:83321966-83321985 | MS.gene99465:CDS | 35.0% |
| !! | CTAGAGAAGGTGCATATTTT+CGG | + | chr4.2:83321483-83321502 | None:intergenic | 35.0% |
| !!! | TCGGATTACATAGTTTTGTG+TGG | + | chr4.2:83321464-83321483 | None:intergenic | 35.0% |
| !!! | TTGGAGATTTTTTGTTGGGT+AGG | - | chr4.2:83321920-83321939 | MS.gene99465:CDS | 35.0% |
| AACATTGGAATTGTGATCGC+AGG | + | chr4.2:83321955-83321974 | None:intergenic | 40.0% | |
| ACCTAAGCCTATTGATGAGA+GGG | - | chr4.2:83321768-83321787 | MS.gene99465:CDS | 40.0% | |
| AGGAAGGTGGGAAGAAATAT+GGG | - | chr4.2:83321803-83321822 | MS.gene99465:CDS | 40.0% | |
| AGGTTGTGTTTGGAGAAAGA+TGG | - | chr4.2:83321886-83321905 | MS.gene99465:CDS | 40.0% | |
| ATCACAACCCTCTCATCAAT+AGG | + | chr4.2:83321778-83321797 | None:intergenic | 40.0% | |
| CTGATTTCAGCTACTAGAGA+AGG | + | chr4.2:83321496-83321515 | None:intergenic | 40.0% | |
| GACTTCTCCAGTTATCACAA+AGG | - | chr4.2:83321627-83321646 | MS.gene99465:CDS | 40.0% | |
| TACCTAAGCCTATTGATGAG+AGG | - | chr4.2:83321767-83321786 | MS.gene99465:CDS | 40.0% | |
| TCAAAGGAGACAAAGATGGT+GGG | - | chr4.2:83321533-83321552 | MS.gene99465:CDS | 40.0% | |
| TTCAAAGGAGACAAAGATGG+TGG | - | chr4.2:83321532-83321551 | MS.gene99465:CDS | 40.0% | |
| TTGTGATAAGAGAGGAAGGT+GGG | - | chr4.2:83321791-83321810 | MS.gene99465:CDS | 40.0% | |
| ! | AAATATGGGGTGGTGACTTT+TGG | - | chr4.2:83321817-83321836 | MS.gene99465:CDS | 40.0% |
| !! | AGACAAAGATGGTGGGTTTA+AGG | - | chr4.2:83321540-83321559 | MS.gene99465:CDS | 40.0% |
| ACCCTCTCATCAATAGGCTT+AGG | + | chr4.2:83321772-83321791 | None:intergenic | 45.0% | |
| AGGAGAAAGTGGAGAAGTTG+AGG | - | chr4.2:83321866-83321885 | MS.gene99465:CDS | 45.0% | |
| AGGGTTGTGATAAGAGAGGA+AGG | - | chr4.2:83321787-83321806 | MS.gene99465:CDS | 45.0% | |
| ATTGGAATTGTGATCGCAGG+TGG | + | chr4.2:83321952-83321971 | None:intergenic | 45.0% | |
| GAGGAAGGTGGGAAGAAATA+TGG | - | chr4.2:83321802-83321821 | MS.gene99465:CDS | 45.0% | |
| GGAAGGTGGGAAGAAATATG+GGG | - | chr4.2:83321804-83321823 | MS.gene99465:CDS | 45.0% | |
| GGAGAAGTTGAGGTTGTGTT+TGG | - | chr4.2:83321876-83321895 | MS.gene99465:CDS | 45.0% | |
| GTTGTGATAAGAGAGGAAGG+TGG | - | chr4.2:83321790-83321809 | MS.gene99465:CDS | 45.0% | |
| TGAAGTGGTGAAGGAGAAAG+TGG | - | chr4.2:83321855-83321874 | MS.gene99465:CDS | 45.0% | |
| TGAGAGGGTTGTGATAAGAG+AGG | - | chr4.2:83321783-83321802 | MS.gene99465:CDS | 45.0% | |
| AGGAGTGGCAAGTGATGAAG+TGG | - | chr4.2:83321840-83321859 | MS.gene99465:CDS | 50.0% | |
| AGGTGGGAAGAAATATGGGG+TGG | - | chr4.2:83321807-83321826 | MS.gene99465:CDS | 50.0% | |
| GGCAAGTGATGAAGTGGTGA+AGG | - | chr4.2:83321846-83321865 | MS.gene99465:CDS | 50.0% | |
| ! | TATGGGGTGGTGACTTTTGG+AGG | - | chr4.2:83321820-83321839 | MS.gene99465:CDS | 50.0% |
| !! | GGTGGTGACTTTTGGAGGAG+TGG | - | chr4.2:83321825-83321844 | MS.gene99465:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 83321403 | 83322008 | 83321403 | ID=MS.gene99465 |
| chr4.2 | mRNA | 83321403 | 83322008 | 83321403 | ID=MS.gene99465.t1;Parent=MS.gene99465 |
| chr4.2 | exon | 83321403 | 83322008 | 83321403 | ID=MS.gene99465.t1.exon1;Parent=MS.gene99465.t1 |
| chr4.2 | CDS | 83321403 | 83322008 | 83321403 | ID=cds.MS.gene99465.t1;Parent=MS.gene99465.t1 |
| Gene Sequence |
| Protein sequence |
