Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003634.01.T01 | XP_024634632.1 | 98.343 | 181 | 3 | 0 | 1 | 181 | 96 | 276 | 1.07E-128 | 372 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003634.01.T01 | Q8GXF8 | 49.223 | 193 | 82 | 3 | 1 | 181 | 93 | 281 | 1.08E-56 | 182 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003634.01.T01 | A0A396JUE4 | 98.343 | 181 | 3 | 0 | 1 | 181 | 96 | 276 | 5.09e-129 | 372 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048068.01 | MsG0180003634.01 | 0.804598 | 2.000094e-49 | 1.035514e-46 |
| MsG0080048845.01 | MsG0180003634.01 | 0.802255 | 6.119629e-49 | 2.983754e-46 |
| MsG0080048969.01 | MsG0180003634.01 | 0.811047 | 8.512948e-51 | 5.215635e-48 |
| MsG0180000502.01 | MsG0180003634.01 | 0.801946 | 7.082497e-49 | 3.426036e-46 |
| MsG0180001685.01 | MsG0180003634.01 | 0.816676 | 4.891299e-52 | 3.488428e-49 |
| MsG0180003146.01 | MsG0180003634.01 | 0.804018 | 2.642025e-49 | 1.347646e-46 |
| MsG0180003442.01 | MsG0180003634.01 | 0.808188 | 3.501203e-50 | 1.988877e-47 |
| MsG0180003451.01 | MsG0180003634.01 | 0.811490 | 6.821798e-51 | 4.228639e-48 |
| MsG0180003634.01 | MsG0180003906.01 | 0.806975 | 6.336049e-50 | 3.486824e-47 |
| MsG0180003634.01 | MsG0180004458.01 | 0.802446 | 5.588520e-49 | 2.738128e-46 |
| MsG0180003634.01 | MsG0180004987.01 | 0.804273 | 2.338033e-49 | 1.200384e-46 |
| MsG0180003634.01 | MsG0180005119.01 | 0.815104 | 1.096917e-51 | 7.493233e-49 |
| MsG0180003634.01 | MsG0180005150.01 | 0.800490 | 1.406576e-48 | 6.558035e-46 |
| MsG0180003634.01 | MsG0180005568.01 | 0.870799 | 1.110504e-66 | 4.376266e-63 |
| MsG0180003634.01 | MsG0280006349.01 | 0.826088 | 3.286427e-54 | 3.051407e-51 |
| MsG0180003634.01 | MsG0280006848.01 | 0.802526 | 5.380133e-49 | 2.641299e-46 |
| MsG0180003634.01 | MsG0280010974.01 | 0.800159 | 1.642676e-48 | 7.594433e-46 |
| MsG0180003634.01 | MsG0280010975.01 | 0.814459 | 1.523887e-51 | 1.023130e-48 |
| MsG0180003634.01 | MsG0280010998.01 | 0.811045 | 8.517766e-51 | 5.218403e-48 |
| MsG0180003634.01 | MsG0280011270.01 | 0.826549 | 2.552040e-54 | 2.400828e-51 |
| MsG0180003634.01 | MsG0380012443.01 | 0.800212 | 1.602475e-48 | 7.418821e-46 |
| MsG0180003634.01 | MsG0380013499.01 | 0.817589 | 3.049326e-52 | 2.229819e-49 |
| MsG0180003634.01 | MsG0380014022.01 | 0.802631 | 5.119356e-49 | 2.520073e-46 |
| MsG0180003634.01 | MsG0380014729.01 | 0.803324 | 3.681678e-49 | 1.844639e-46 |
| MsG0180003634.01 | MsG0380015921.01 | 0.802108 | 6.560805e-49 | 3.186851e-46 |
| MsG0180003634.01 | MsG0380016399.01 | 0.832077 | 1.161184e-55 | 1.284116e-52 |
| MsG0180003634.01 | MsG0380016459.01 | 0.822479 | 2.317963e-53 | 1.942436e-50 |
| MsG0180003634.01 | MsG0380016751.01 | 0.803316 | 3.695298e-49 | 1.851100e-46 |
| MsG0180003634.01 | MsG0380017739.01 | 0.806979 | 6.322690e-50 | 3.479861e-47 |
| MsG0180003634.01 | MsG0480018089.01 | 0.819729 | 9.967577e-53 | 7.733051e-50 |
| MsG0180003634.01 | MsG0480018323.01 | 0.805878 | 1.079302e-49 | 5.774977e-47 |
| MsG0180003634.01 | MsG0480018844.01 | 0.815022 | 1.143830e-51 | 7.796907e-49 |
| MsG0180003634.01 | MsG0480018983.01 | 0.808803 | 2.588552e-50 | 1.494193e-47 |
| MsG0180003634.01 | MsG0480019592.01 | 0.801931 | 7.134358e-49 | 3.449842e-46 |
| MsG0180003634.01 | MsG0480019693.01 | 0.816395 | 5.654806e-52 | 4.002158e-49 |
| MsG0180003634.01 | MsG0480020229.01 | 0.804073 | 2.573450e-49 | 1.314547e-46 |
| MsG0180003634.01 | MsG0480020350.01 | 0.800543 | 1.371774e-48 | 6.404329e-46 |
| MsG0180003634.01 | MsG0480021367.01 | 0.806969 | 6.354282e-50 | 3.496340e-47 |
| MsG0180003634.01 | MsG0480021420.01 | 0.825254 | 5.183054e-54 | 4.699646e-51 |
| MsG0180003634.01 | MsG0480023166.01 | 0.806552 | 7.783607e-50 | 4.237130e-47 |
| MsG0180003634.01 | MsG0480023422.01 | 0.803095 | 4.105868e-49 | 2.045072e-46 |
| MsG0180003634.01 | MsG0480023708.01 | 0.815159 | 1.066597e-51 | 7.297133e-49 |
| MsG0180003634.01 | MsG0480023991.01 | 0.807709 | 4.427639e-50 | 2.483542e-47 |
| MsG0180003634.01 | MsG0580024759.01 | 0.805249 | 1.462506e-49 | 7.699638e-47 |
| MsG0180003634.01 | MsG0580024976.01 | -0.812129 | 4.951963e-51 | 3.122420e-48 |
| MsG0180003634.01 | MsG0580025589.01 | 0.813815 | 2.114277e-51 | 1.394922e-48 |
| MsG0180003634.01 | MsG0580027098.01 | 0.814028 | 1.897494e-51 | 1.259144e-48 |
| MsG0180003634.01 | MsG0580028925.01 | 0.800212 | 1.602475e-48 | 7.418821e-46 |
| MsG0180003634.01 | MsG0680033902.01 | 0.800800 | 1.215843e-48 | 5.713414e-46 |
| MsG0180003634.01 | MsG0780037946.01 | 0.813930 | 1.994813e-51 | 1.320132e-48 |
| MsG0180003634.01 | MsG0780038986.01 | 0.814017 | 1.908130e-51 | 1.265839e-48 |
| MsG0180003634.01 | MsG0780039859.01 | 0.805811 | 1.114518e-49 | 5.953344e-47 |
| MsG0180003634.01 | MsG0780040343.01 | 0.806224 | 9.127962e-50 | 4.927600e-47 |
| MsG0180003634.01 | MsG0780040417.01 | 0.815123 | 1.086393e-51 | 7.425321e-49 |
| MsG0180003634.01 | MsG0780040484.01 | 0.805313 | 1.418020e-49 | 7.477965e-47 |
| MsG0180003634.01 | MsG0780041706.01 | 0.809934 | 1.480092e-50 | 8.805006e-48 |
| MsG0180003634.01 | MsG0880042343.01 | 0.818118 | 2.315418e-52 | 1.717968e-49 |
| MsG0180003634.01 | MsG0880045261.01 | 0.841219 | 5.425160e-58 | 7.916760e-55 |
| MsG0180003634.01 | MsG0880045283.01 | 0.827648 | 1.392746e-54 | 1.352718e-51 |
| MsG0180003634.01 | MsG0880045412.01 | 0.811277 | 7.585803e-51 | 4.675558e-48 |
| MsG0180003634.01 | MsG0880046288.01 | 0.820791 | 5.691886e-53 | 4.547982e-50 |
| MsG0180003634.01 | MsG0880046456.01 | 0.803081 | 4.132059e-49 | 2.057410e-46 |
| MsG0180003634.01 | MsG0880046735.01 | 0.807930 | 3.973380e-50 | 2.241566e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003634.01.T01 | MTR_1g067260 | 98.425 | 127 | 2 | 0 | 1 | 127 | 96 | 222 | 3.14e-85 | 253 |
| MsG0180003634.01.T01 | MTR_7g074170 | 39.823 | 113 | 58 | 3 | 55 | 162 | 286 | 393 | 4.48e-18 | 81.3 |
| MsG0180003634.01.T01 | MTR_2g097620 | 47.222 | 72 | 34 | 1 | 94 | 161 | 165 | 236 | 2.52e-17 | 78.6 |
| MsG0180003634.01.T01 | MTR_1g114620 | 49.296 | 71 | 34 | 1 | 94 | 162 | 242 | 312 | 3.50e-17 | 78.2 |
| MsG0180003634.01.T01 | MTR_6g079660 | 41.892 | 74 | 38 | 1 | 94 | 162 | 210 | 283 | 1.18e-15 | 73.9 |
| MsG0180003634.01.T01 | MTR_4g110100 | 48.000 | 75 | 34 | 1 | 94 | 163 | 335 | 409 | 1.62e-15 | 73.9 |
| MsG0180003634.01.T01 | MTR_5g041310 | 36.735 | 98 | 59 | 2 | 65 | 161 | 67 | 162 | 1.89e-15 | 72.0 |
| MsG0180003634.01.T01 | MTR_4g064570 | 48.611 | 72 | 33 | 1 | 94 | 161 | 157 | 228 | 4.09e-15 | 72.4 |
| MsG0180003634.01.T01 | MTR_5g041330 | 42.857 | 70 | 39 | 1 | 94 | 162 | 84 | 153 | 4.22e-15 | 70.9 |
| MsG0180003634.01.T01 | MTR_6g016350 | 57.143 | 49 | 21 | 0 | 114 | 162 | 311 | 359 | 9.18e-15 | 71.6 |
| MsG0180003634.01.T01 | MTR_1g114320 | 35.484 | 93 | 55 | 1 | 76 | 163 | 151 | 243 | 3.42e-14 | 69.7 |
| MsG0180003634.01.T01 | MTR_3g068005 | 44.737 | 76 | 38 | 1 | 94 | 165 | 172 | 247 | 4.41e-14 | 69.7 |
| MsG0180003634.01.T01 | MTR_1g051105 | 31.959 | 97 | 60 | 2 | 94 | 186 | 135 | 229 | 3.62e-13 | 66.2 |
| MsG0180003634.01.T01 | MTR_5g080350 | 37.500 | 80 | 46 | 1 | 84 | 159 | 159 | 238 | 4.01e-13 | 67.0 |
| MsG0180003634.01.T01 | MTR_1g014710 | 40.789 | 76 | 38 | 2 | 94 | 162 | 206 | 281 | 1.44e-12 | 65.1 |
| MsG0180003634.01.T01 | MTR_5g088720 | 34.286 | 70 | 42 | 1 | 94 | 159 | 222 | 291 | 4.77e-12 | 63.9 |
| MsG0180003634.01.T01 | MTR_5g088720 | 34.286 | 70 | 42 | 1 | 94 | 159 | 181 | 250 | 6.85e-12 | 63.2 |
| MsG0180003634.01.T01 | MTR_2g007340 | 45.588 | 68 | 33 | 1 | 99 | 162 | 182 | 249 | 6.94e-12 | 63.2 |
| MsG0180003634.01.T01 | MTR_5g088720 | 34.286 | 70 | 42 | 1 | 94 | 159 | 176 | 245 | 7.05e-12 | 63.2 |
| MsG0180003634.01.T01 | MTR_3g083480 | 40.580 | 69 | 40 | 1 | 94 | 162 | 218 | 285 | 2.45e-11 | 61.6 |
| MsG0180003634.01.T01 | MTR_3g088080 | 37.209 | 86 | 47 | 2 | 77 | 160 | 4 | 84 | 4.20e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003634.01.T01 | AT5G02750 | 49.223 | 193 | 82 | 3 | 1 | 181 | 93 | 281 | 1.10e-57 | 182 |
| MsG0180003634.01.T01 | AT5G60820 | 68.750 | 48 | 15 | 0 | 114 | 161 | 372 | 419 | 8.76e-19 | 83.2 |
| MsG0180003634.01.T01 | AT3G02340 | 35.556 | 135 | 79 | 2 | 37 | 163 | 250 | 384 | 2.45e-18 | 82.0 |
| MsG0180003634.01.T01 | AT3G02340 | 35.556 | 135 | 79 | 2 | 37 | 163 | 250 | 384 | 2.45e-18 | 82.0 |
| MsG0180003634.01.T01 | AT5G08139 | 50.000 | 64 | 32 | 0 | 99 | 162 | 294 | 357 | 3.30e-17 | 78.6 |
| MsG0180003634.01.T01 | AT3G19950 | 36.957 | 92 | 53 | 1 | 76 | 162 | 173 | 264 | 5.22e-17 | 77.8 |
| MsG0180003634.01.T01 | AT3G19950 | 36.957 | 92 | 53 | 1 | 76 | 162 | 231 | 322 | 6.07e-17 | 77.8 |
| MsG0180003634.01.T01 | AT3G19950 | 36.957 | 92 | 53 | 1 | 76 | 162 | 231 | 322 | 6.07e-17 | 77.8 |
| MsG0180003634.01.T01 | AT5G15820 | 45.679 | 81 | 39 | 1 | 94 | 169 | 266 | 346 | 2.09e-16 | 76.3 |
| MsG0180003634.01.T01 | AT3G46620 | 45.946 | 74 | 36 | 1 | 94 | 163 | 191 | 264 | 1.05e-15 | 74.3 |
| MsG0180003634.01.T01 | AT1G26800 | 29.570 | 186 | 106 | 5 | 1 | 183 | 21 | 184 | 2.40e-15 | 71.2 |
| MsG0180003634.01.T01 | AT5G59550 | 47.222 | 72 | 34 | 2 | 94 | 161 | 280 | 351 | 6.37e-15 | 72.4 |
| MsG0180003634.01.T01 | AT5G59550 | 47.222 | 72 | 34 | 2 | 94 | 161 | 175 | 246 | 1.00e-14 | 71.6 |
| MsG0180003634.01.T01 | AT5G64920 | 42.500 | 80 | 42 | 1 | 94 | 169 | 233 | 312 | 1.14e-14 | 71.2 |
| MsG0180003634.01.T01 | AT3G10815 | 36.905 | 84 | 49 | 1 | 94 | 173 | 97 | 180 | 1.58e-14 | 68.9 |
| MsG0180003634.01.T01 | AT5G01980 | 45.205 | 73 | 36 | 1 | 94 | 162 | 326 | 398 | 1.65e-14 | 71.2 |
| MsG0180003634.01.T01 | AT1G60360 | 45.070 | 71 | 34 | 1 | 94 | 159 | 199 | 269 | 7.25e-14 | 68.9 |
| MsG0180003634.01.T01 | AT5G20910 | 54.000 | 50 | 23 | 0 | 113 | 162 | 229 | 278 | 8.79e-14 | 68.6 |
| MsG0180003634.01.T01 | AT2G39720 | 39.474 | 76 | 40 | 1 | 94 | 163 | 175 | 250 | 5.14e-13 | 66.6 |
| MsG0180003634.01.T01 | AT2G39720 | 39.474 | 76 | 40 | 1 | 94 | 163 | 175 | 250 | 5.14e-13 | 66.6 |
| MsG0180003634.01.T01 | AT4G26400 | 42.029 | 69 | 39 | 1 | 94 | 162 | 222 | 289 | 8.21e-13 | 65.9 |
| MsG0180003634.01.T01 | AT4G26400 | 42.029 | 69 | 39 | 1 | 94 | 162 | 222 | 289 | 8.21e-13 | 65.9 |
| MsG0180003634.01.T01 | AT3G60080 | 40.000 | 65 | 39 | 0 | 114 | 178 | 169 | 233 | 5.29e-12 | 63.5 |
| MsG0180003634.01.T01 | AT3G13430 | 36.232 | 69 | 44 | 0 | 94 | 162 | 205 | 273 | 5.54e-12 | 63.5 |
| MsG0180003634.01.T01 | AT3G13430 | 36.232 | 69 | 44 | 0 | 94 | 162 | 205 | 273 | 5.54e-12 | 63.5 |
| MsG0180003634.01.T01 | AT3G13430 | 36.232 | 69 | 44 | 0 | 94 | 162 | 205 | 273 | 5.54e-12 | 63.5 |
| MsG0180003634.01.T01 | AT3G13430 | 36.232 | 69 | 44 | 0 | 94 | 162 | 205 | 273 | 5.54e-12 | 63.5 |
| MsG0180003634.01.T01 | AT2G40830 | 34.722 | 72 | 42 | 1 | 94 | 160 | 165 | 236 | 5.75e-12 | 63.5 |
| MsG0180003634.01.T01 | AT2G40830 | 34.722 | 72 | 42 | 1 | 94 | 160 | 165 | 236 | 5.75e-12 | 63.5 |
| MsG0180003634.01.T01 | AT2G40830 | 34.722 | 72 | 42 | 1 | 94 | 160 | 165 | 236 | 5.75e-12 | 63.5 |
| MsG0180003634.01.T01 | AT2G40830 | 34.722 | 72 | 42 | 1 | 94 | 160 | 165 | 236 | 5.75e-12 | 63.5 |
| MsG0180003634.01.T01 | AT2G44330 | 52.174 | 46 | 22 | 0 | 114 | 159 | 96 | 141 | 1.98e-11 | 60.5 |
| MsG0180003634.01.T01 | AT5G56340 | 40.299 | 67 | 39 | 1 | 94 | 160 | 240 | 305 | 2.04e-11 | 62.0 |
| MsG0180003634.01.T01 | AT3G56580 | 32.394 | 71 | 43 | 1 | 94 | 159 | 161 | 231 | 8.32e-11 | 60.1 |
| MsG0180003634.01.T01 | AT3G56580 | 32.394 | 71 | 43 | 1 | 94 | 159 | 161 | 231 | 8.32e-11 | 60.1 |
| MsG0180003634.01.T01 | AT3G56580 | 32.394 | 71 | 43 | 1 | 94 | 159 | 161 | 231 | 8.32e-11 | 60.1 |
| MsG0180003634.01.T01 | AT1G18770 | 47.170 | 53 | 28 | 0 | 107 | 159 | 52 | 104 | 9.29e-11 | 57.0 |
Find 51 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAGATTGGTGGGTGTTT+TGG | 0.137532 | 1:-65520615 | MsG0180003634.01.T01:CDS |
| AACGGTGCGGTTAATGCTTC+TGG | 0.240119 | 1:+65520770 | None:intergenic |
| CCACCGCTTGGTGCATGTTT+TGG | 0.252463 | 1:-65520677 | MsG0180003634.01.T01:CDS |
| CCAACGGTTGTGGGAAGTTT+TGG | 0.260265 | 1:-65520336 | MsG0180003634.01.T01:CDS |
| CATGTCAGCATTTGTTTCAT+TGG | 0.329624 | 1:-65520445 | MsG0180003634.01.T01:CDS |
| AGTGAAGGAGGTGGCGGCTT+CGG | 0.342382 | 1:-65520573 | MsG0180003634.01.T01:CDS |
| GGAACACGTGTCCTTGTTGC+AGG | 0.342454 | 1:-65520394 | MsG0180003634.01.T01:CDS |
| CGTGTCCTTGTTGCAGGTTT+CGG | 0.355293 | 1:-65520388 | MsG0180003634.01.T01:CDS |
| GGCGACGGTGGTTTCTTTGC+CGG | 0.368886 | 1:-65520549 | MsG0180003634.01.T01:CDS |
| CGGCTCACCGTCTCGATAAA+CGG | 0.399480 | 1:+65520641 | None:intergenic |
| TCTTCTCCAACACATTGTTA+AGG | 0.406100 | 1:-65520718 | MsG0180003634.01.T01:CDS |
| GTAAAGAAGAGATGCAAGTA+GGG | 0.417369 | 1:-65520487 | MsG0180003634.01.T01:CDS |
| GGTTTCTTTGCCGGCGGTGG+AGG | 0.424492 | 1:-65520540 | MsG0180003634.01.T01:CDS |
| GGTGGTTTCTTTGCCGGCGG+TGG | 0.426920 | 1:-65520543 | MsG0180003634.01.T01:CDS |
| TTGGAGAGATCCAACGGTTG+TGG | 0.429327 | 1:-65520346 | MsG0180003634.01.T01:CDS |
| GCGGTGGAGGTGAGTGTTGG+TGG | 0.436431 | 1:-65520527 | MsG0180003634.01.T01:CDS |
| AAATTAGTGCTAAACATAAC+AGG | 0.443732 | 1:-65520310 | MsG0180003634.01.T01:CDS |
| TATTCTTCTGCACCACCGCT+TGG | 0.446476 | 1:-65520689 | MsG0180003634.01.T01:CDS |
| GGTGGCGGCTTCGGCGGCGA+CGG | 0.468504 | 1:-65520564 | MsG0180003634.01.T01:CDS |
| AGAGGGGAAGGAAGTGAAGG+AGG | 0.486989 | 1:-65520585 | MsG0180003634.01.T01:CDS |
| TGTAAAGAAGAGATGCAAGT+AGG | 0.495873 | 1:-65520488 | MsG0180003634.01.T01:CDS |
| TGGAGAGATCCAACGGTTGT+GGG | 0.497245 | 1:-65520345 | MsG0180003634.01.T01:CDS |
| TTTAGCCTCCAGTGTGAAAA+CGG | 0.505498 | 1:+65520752 | None:intergenic |
| TGAAGAGGGGAAGGAAGTGA+AGG | 0.505805 | 1:-65520588 | MsG0180003634.01.T01:CDS |
| CCGGCGGTGGAGGTGAGTGT+TGG | 0.507563 | 1:-65520530 | MsG0180003634.01.T01:CDS |
| GAGCCGGTGTTGGAGATTGG+TGG | 0.508330 | 1:-65520624 | MsG0180003634.01.T01:CDS |
| AGCCGGTGTTGGAGATTGGT+GGG | 0.508824 | 1:-65520623 | MsG0180003634.01.T01:CDS |
| CAATGAAACAAATGCTGACA+TGG | 0.516627 | 1:+65520446 | None:intergenic |
| GGTGAGCCGGTGTTGGAGAT+TGG | 0.519084 | 1:-65520627 | MsG0180003634.01.T01:CDS |
| GGCGGCTTCGGCGGCGACGG+TGG | 0.523068 | 1:-65520561 | MsG0180003634.01.T01:CDS |
| GAAGGAAGTGAAGGAGGTGG+CGG | 0.525705 | 1:-65520579 | MsG0180003634.01.T01:CDS |
| GGGGAAGGAAGTGAAGGAGG+TGG | 0.525875 | 1:-65520582 | MsG0180003634.01.T01:CDS |
| GTCGGTGTCACGTTCTCTCA+TGG | 0.527329 | 1:+65520838 | None:intergenic |
| CCAACACTCACCTCCACCGC+CGG | 0.535946 | 1:+65520530 | None:intergenic |
| CGGTGGAGGTGAGTGTTGGT+GGG | 0.538337 | 1:-65520526 | MsG0180003634.01.T01:CDS |
| TCGAGACGGTGAGCCGGTGT+TGG | 0.538693 | 1:-65520634 | MsG0180003634.01.T01:CDS |
| CCAAAACTTCCCACAACCGT+TGG | 0.540607 | 1:+65520336 | None:intergenic |
| TTTGGGTTGTGAAGAGGGGA+AGG | 0.541948 | 1:-65520597 | MsG0180003634.01.T01:CDS |
| AGAAAGCCTTAACAATGTGT+TGG | 0.545710 | 1:+65520712 | None:intergenic |
| GAAGGAGGTGGCGGCTTCGG+CGG | 0.550251 | 1:-65520570 | MsG0180003634.01.T01:CDS |
| CACCCACCAATCTCCAACAC+CGG | 0.558850 | 1:+65520621 | None:intergenic |
| TTTGATTCCGTTTATCGAGA+CGG | 0.586178 | 1:-65520648 | MsG0180003634.01.T01:CDS |
| CGTTTATCGAGACGGTGAGC+CGG | 0.603190 | 1:-65520640 | MsG0180003634.01.T01:CDS |
| GACGGTGGTTTCTTTGCCGG+CGG | 0.603871 | 1:-65520546 | MsG0180003634.01.T01:CDS |
| GAGAAGGAGTAGCACAGCGT+CGG | 0.614727 | 1:+65520820 | None:intergenic |
| GTGTTCCTCTTTCCCAACCA+TGG | 0.628021 | 1:+65520410 | None:intergenic |
| GTGTTTCACAGAACAAGAGA+AGG | 0.630662 | 1:+65520804 | None:intergenic |
| CCAAAACATGCACCAAGCGG+TGG | 0.645373 | 1:+65520677 | None:intergenic |
| GGTAACCGAAACCTGCAACA+AGG | 0.660646 | 1:+65520383 | None:intergenic |
| GATCCAAAACATGCACCAAG+CGG | 0.721251 | 1:+65520674 | None:intergenic |
| CCTCCAGTGTGAAAACGGTG+CGG | 0.730782 | 1:+65520757 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTAGTGCTAAACATAAC+AGG | - | Chr1:65520824-65520843 | MsG0180003634.01.T01:CDS | 25.0% |
| ! | ATGTGTATTTTACCATGGTT+GGG | - | Chr1:65520712-65520731 | MsG0180003634.01.T01:CDS | 30.0% |
| ! | ATTGGATGTGTATTTTACCA+TGG | - | Chr1:65520707-65520726 | MsG0180003634.01.T01:CDS | 30.0% |
| AGAAAGCCTTAACAATGTGT+TGG | + | Chr1:65520425-65520444 | None:intergenic | 35.0% | |
| CAATGAAACAAATGCTGACA+TGG | + | Chr1:65520691-65520710 | None:intergenic | 35.0% | |
| CATGTCAGCATTTGTTTCAT+TGG | - | Chr1:65520689-65520708 | MsG0180003634.01.T01:CDS | 35.0% | |
| GATCTCTCCAAAAACATCAT+CGG | + | Chr1:65520779-65520798 | None:intergenic | 35.0% | |
| GTAAAGAAGAGATGCAAGTA+GGG | - | Chr1:65520647-65520666 | MsG0180003634.01.T01:CDS | 35.0% | |
| TCTTCTCCAACACATTGTTA+AGG | - | Chr1:65520416-65520435 | MsG0180003634.01.T01:CDS | 35.0% | |
| TGTAAAGAAGAGATGCAAGT+AGG | - | Chr1:65520646-65520665 | MsG0180003634.01.T01:CDS | 35.0% | |
| TTTGATTCCGTTTATCGAGA+CGG | - | Chr1:65520486-65520505 | MsG0180003634.01.T01:CDS | 35.0% | |
| ! | GATGTGTATTTTACCATGGT+TGG | - | Chr1:65520711-65520730 | MsG0180003634.01.T01:CDS | 35.0% |
| !!! | ATGTTTTTGGAGAGATCCAA+CGG | - | Chr1:65520782-65520801 | MsG0180003634.01.T01:CDS | 35.0% |
| GTGTTTCACAGAACAAGAGA+AGG | + | Chr1:65520333-65520352 | None:intergenic | 40.0% | |
| TCTCCAAAAACATCATCGGA+CGG | + | Chr1:65520775-65520794 | None:intergenic | 40.0% | |
| TTTAGCCTCCAGTGTGAAAA+CGG | + | Chr1:65520385-65520404 | None:intergenic | 40.0% | |
| ! | TTACCGTCCGATGATGTTTT+TGG | - | Chr1:65520769-65520788 | MsG0180003634.01.T01:CDS | 40.0% |
| ! | TTTTACCATGGTTGGGAAAG+AGG | - | Chr1:65520719-65520738 | MsG0180003634.01.T01:CDS | 40.0% |
| GATCCAAAACATGCACCAAG+CGG | + | Chr1:65520463-65520482 | None:intergenic | 45.0% | |
| !! | TGGAGATTGGTGGGTGTTTT+GGG | - | Chr1:65520520-65520539 | MsG0180003634.01.T01:CDS | 45.0% |
| !! | TTGGAGATTGGTGGGTGTTT+TGG | - | Chr1:65520519-65520538 | MsG0180003634.01.T01:CDS | 45.0% |
| !!! | GCTTGGTGCATGTTTTGGAT+CGG | - | Chr1:65520462-65520481 | MsG0180003634.01.T01:CDS | 45.0% |
| !!! | GGTGTTTTGGGTTGTGAAGA+GGG | - | Chr1:65520532-65520551 | MsG0180003634.01.T01:CDS | 45.0% |
| !!! | GTGTTTTGGGTTGTGAAGAG+GGG | - | Chr1:65520533-65520552 | MsG0180003634.01.T01:CDS | 45.0% |
| AACGGTGCGGTTAATGCTTC+TGG | + | Chr1:65520367-65520386 | None:intergenic | 50.0% | |
| CCAAAACTTCCCACAACCGT+TGG | + | Chr1:65520801-65520820 | None:intergenic | 50.0% | |
| CGTGTCCTTGTTGCAGGTTT+CGG | - | Chr1:65520746-65520765 | MsG0180003634.01.T01:CDS | 50.0% | |
| GGTAACCGAAACCTGCAACA+AGG | + | Chr1:65520754-65520773 | None:intergenic | 50.0% | |
| GTGTTCCTCTTTCCCAACCA+TGG | + | Chr1:65520727-65520746 | None:intergenic | 50.0% | |
| TATTCTTCTGCACCACCGCT+TGG | - | Chr1:65520445-65520464 | MsG0180003634.01.T01:CDS | 50.0% | |
| TGAAGAGGGGAAGGAAGTGA+AGG | - | Chr1:65520546-65520565 | MsG0180003634.01.T01:CDS | 50.0% | |
| TGGAGAGATCCAACGGTTGT+GGG | - | Chr1:65520789-65520808 | MsG0180003634.01.T01:CDS | 50.0% | |
| TTGGAGAGATCCAACGGTTG+TGG | - | Chr1:65520788-65520807 | MsG0180003634.01.T01:CDS | 50.0% | |
| ! | CCAACGGTTGTGGGAAGTTT+TGG | - | Chr1:65520798-65520817 | MsG0180003634.01.T01:CDS | 50.0% |
| ! | TAACCGCACCGTTTTCACAC+TGG | - | Chr1:65520374-65520393 | MsG0180003634.01.T01:CDS | 50.0% |
| !! | TTTGGGTTGTGAAGAGGGGA+AGG | - | Chr1:65520537-65520556 | MsG0180003634.01.T01:CDS | 50.0% |
| !!! | GGGTGTTTTGGGTTGTGAAG+AGG | - | Chr1:65520531-65520550 | MsG0180003634.01.T01:CDS | 50.0% |
| AGAGGGGAAGGAAGTGAAGG+AGG | - | Chr1:65520549-65520568 | MsG0180003634.01.T01:CDS | 55.0% | |
| CACCCACCAATCTCCAACAC+CGG | + | Chr1:65520516-65520535 | None:intergenic | 55.0% | |
| CCAAAACATGCACCAAGCGG+TGG | + | Chr1:65520460-65520479 | None:intergenic | 55.0% | |
| CCTCCAGTGTGAAAACGGTG+CGG | + | Chr1:65520380-65520399 | None:intergenic | 55.0% | |
| CGGCTCACCGTCTCGATAAA+CGG | + | Chr1:65520496-65520515 | None:intergenic | 55.0% | |
| CGTTTATCGAGACGGTGAGC+CGG | - | Chr1:65520494-65520513 | MsG0180003634.01.T01:CDS | 55.0% | |
| GAAGGAAGTGAAGGAGGTGG+CGG | - | Chr1:65520555-65520574 | MsG0180003634.01.T01:CDS | 55.0% | |
| GAGAAGGAGTAGCACAGCGT+CGG | + | Chr1:65520317-65520336 | None:intergenic | 55.0% | |
| GGAACACGTGTCCTTGTTGC+AGG | - | Chr1:65520740-65520759 | MsG0180003634.01.T01:CDS | 55.0% | |
| ! | AGCCGGTGTTGGAGATTGGT+GGG | - | Chr1:65520511-65520530 | MsG0180003634.01.T01:CDS | 55.0% |
| !!! | CCACCGCTTGGTGCATGTTT+TGG | - | Chr1:65520457-65520476 | MsG0180003634.01.T01:CDS | 55.0% |
| GACGGTGGTTTCTTTGCCGG+CGG | - | Chr1:65520588-65520607 | MsG0180003634.01.T01:CDS | 60.0% | |
| GGCGACGGTGGTTTCTTTGC+CGG | - | Chr1:65520585-65520604 | MsG0180003634.01.T01:CDS | 60.0% | |
| GGGGAAGGAAGTGAAGGAGG+TGG | - | Chr1:65520552-65520571 | MsG0180003634.01.T01:CDS | 60.0% | |
| ! | AGTGAAGGAGGTGGCGGCTT+CGG | - | Chr1:65520561-65520580 | MsG0180003634.01.T01:CDS | 60.0% |
| ! | CCGCACCGTTTTCACACTGG+AGG | - | Chr1:65520377-65520396 | MsG0180003634.01.T01:CDS | 60.0% |
| ! | GAGCCGGTGTTGGAGATTGG+TGG | - | Chr1:65520510-65520529 | MsG0180003634.01.T01:CDS | 60.0% |
| ! | GGTGAGCCGGTGTTGGAGAT+TGG | - | Chr1:65520507-65520526 | MsG0180003634.01.T01:CDS | 60.0% |
| !! | CGGTGGAGGTGAGTGTTGGT+GGG | - | Chr1:65520608-65520627 | MsG0180003634.01.T01:CDS | 60.0% |
| CCAACACTCACCTCCACCGC+CGG | + | Chr1:65520607-65520626 | None:intergenic | 65.0% | |
| GGTGGTTTCTTTGCCGGCGG+TGG | - | Chr1:65520591-65520610 | MsG0180003634.01.T01:CDS | 65.0% | |
| GGTTTCTTTGCCGGCGGTGG+AGG | - | Chr1:65520594-65520613 | MsG0180003634.01.T01:CDS | 65.0% | |
| TCGAGACGGTGAGCCGGTGT+TGG | - | Chr1:65520500-65520519 | MsG0180003634.01.T01:CDS | 65.0% | |
| !! | GCGGTGGAGGTGAGTGTTGG+TGG | - | Chr1:65520607-65520626 | MsG0180003634.01.T01:CDS | 65.0% |
| CCGGCGGTGGAGGTGAGTGT+TGG | - | Chr1:65520604-65520623 | MsG0180003634.01.T01:CDS | 70.0% | |
| ! | GAAGGAGGTGGCGGCTTCGG+CGG | - | Chr1:65520564-65520583 | MsG0180003634.01.T01:CDS | 70.0% |
| !! | GGTGGCGGCTTCGGCGGCGA+CGG | - | Chr1:65520570-65520589 | MsG0180003634.01.T01:CDS | 80.0% |
| ! | GGCGGCTTCGGCGGCGACGG+TGG | - | Chr1:65520573-65520592 | MsG0180003634.01.T01:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 65520298 | 65520858 | 65520298 | ID=MsG0180003634.01;Name=MsG0180003634.01 |
| Chr1 | mRNA | 65520298 | 65520858 | 65520298 | ID=MsG0180003634.01.T01;Parent=MsG0180003634.01;Name=MsG0180003634.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|186 |
| Chr1 | exon | 65520298 | 65520858 | 65520298 | ID=MsG0180003634.01.T01:exon:50675;Parent=MsG0180003634.01.T01 |
| Chr1 | CDS | 65520298 | 65520858 | 65520298 | ID=MsG0180003634.01.T01:cds;Parent=MsG0180003634.01.T01 |
| Gene Sequence |
| Protein sequence |
