AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023991.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023991.01.T01 MTR_8g106680 98.765 81 1 0 1 81 672 752 8.18e-47 159
MsG0480023991.01.T01 MTR_6g080430 60.494 81 27 2 1 81 671 746 2.04e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATCAGCGTGACTCTTTC+AGG 0.228861 4:-92106639 None:intergenic
GGAAAGTGGGAAAGATCTTT+TGG 0.322823 4:+92106530 MsG0480023991.01.T01:CDS
TGAAAGAGTCACGCTGATAT+TGG 0.381885 4:+92106641 MsG0480023991.01.T01:CDS
CTGCTATTCCACTTGAGGAT+TGG 0.394911 4:+92106556 MsG0480023991.01.T01:CDS
CTTTGTTGTAGCTTAAGCTT+TGG 0.419370 4:-92106587 None:intergenic
ATCGAATGGAGCTTCTTCTT+CGG 0.429366 4:-92106481 None:intergenic
ACTGCAACTGCAAGTTCTAA+TGG 0.438234 4:+92106612 MsG0480023991.01.T01:CDS
GGCTTCTGCTATTCCACTTG+AGG 0.457095 4:+92106551 MsG0480023991.01.T01:CDS
CTGCACTCAGCTCAAAGAAA+GGG 0.475554 4:+92106508 MsG0480023991.01.T01:CDS
AGCTCAAAGAAAGGGGAAAG+TGG 0.476835 4:+92106516 MsG0480023991.01.T01:CDS
TCTGCACTCAGCTCAAAGAA+AGG 0.515153 4:+92106507 MsG0480023991.01.T01:CDS
TTTCAATGCCTCAACTGCCA+TGG 0.518234 4:-92106442 None:intergenic
TGATATTGGTTGTCAACTGC+GGG 0.521802 4:+92106655 MsG0480023991.01.T01:CDS
AATGCAGAAGATTGAATCCA+TGG 0.535381 4:+92106425 None:intergenic
GATTGAATCCATGGCAGTTG+AGG 0.540044 4:+92106434 MsG0480023991.01.T01:CDS
GCTCAAAGAAAGGGGAAAGT+GGG 0.576184 4:+92106517 MsG0480023991.01.T01:CDS
CTGATATTGGTTGTCAACTG+CGG 0.597414 4:+92106654 MsG0480023991.01.T01:CDS
TGCACTCAGCTCAAAGAAAG+GGG 0.602008 4:+92106509 MsG0480023991.01.T01:CDS
TCTCTAATCCAATCCTCAAG+TGG 0.613755 4:-92106564 None:intergenic
CAAAGCTTAAGCTACAACAA+AGG 0.620042 4:+92106588 MsG0480023991.01.T01:CDS
CTGAGTGCAGAAACATCGAA+TGG 0.625191 4:-92106495 None:intergenic
ATTGAAAATTCAAGCTGACG+TGG 0.632872 4:+92106458 MsG0480023991.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTGAAAATTCAAGCTGACG+TGG + Chr4:92106458-92106477 MsG0480023991.01.T01:CDS 35.0%
CAAAGCTTAAGCTACAACAA+AGG + Chr4:92106588-92106607 MsG0480023991.01.T01:CDS 35.0%
! CTTTGTTGTAGCTTAAGCTT+TGG - Chr4:92106590-92106609 None:intergenic 35.0%
AATATCAGCGTGACTCTTTC+AGG - Chr4:92106642-92106661 None:intergenic 40.0%
ATCGAATGGAGCTTCTTCTT+CGG - Chr4:92106484-92106503 None:intergenic 40.0%
TCTCTAATCCAATCCTCAAG+TGG - Chr4:92106567-92106586 None:intergenic 40.0%
TGAAAGAGTCACGCTGATAT+TGG + Chr4:92106641-92106660 MsG0480023991.01.T01:CDS 40.0%
! ACTGCAACTGCAAGTTCTAA+TGG + Chr4:92106612-92106631 MsG0480023991.01.T01:CDS 40.0%
! CTGATATTGGTTGTCAACTG+CGG + Chr4:92106654-92106673 MsG0480023991.01.T01:CDS 40.0%
! GGAAAGTGGGAAAGATCTTT+TGG + Chr4:92106530-92106549 MsG0480023991.01.T01:CDS 40.0%
! TGATATTGGTTGTCAACTGC+GGG + Chr4:92106655-92106674 MsG0480023991.01.T01:CDS 40.0%
AGCTCAAAGAAAGGGGAAAG+TGG + Chr4:92106516-92106535 MsG0480023991.01.T01:CDS 45.0%
CTGAGTGCAGAAACATCGAA+TGG - Chr4:92106498-92106517 None:intergenic 45.0%
CTGCACTCAGCTCAAAGAAA+GGG + Chr4:92106508-92106527 MsG0480023991.01.T01:CDS 45.0%
CTGCTATTCCACTTGAGGAT+TGG + Chr4:92106556-92106575 MsG0480023991.01.T01:CDS 45.0%
GATTGAATCCATGGCAGTTG+AGG + Chr4:92106434-92106453 MsG0480023991.01.T01:CDS 45.0%
GCTCAAAGAAAGGGGAAAGT+GGG + Chr4:92106517-92106536 MsG0480023991.01.T01:CDS 45.0%
TCTGCACTCAGCTCAAAGAA+AGG + Chr4:92106507-92106526 MsG0480023991.01.T01:CDS 45.0%
TGCACTCAGCTCAAAGAAAG+GGG + Chr4:92106509-92106528 MsG0480023991.01.T01:CDS 45.0%
TTTCAATGCCTCAACTGCCA+TGG - Chr4:92106445-92106464 None:intergenic 45.0%
GGCTTCTGCTATTCCACTTG+AGG + Chr4:92106551-92106570 MsG0480023991.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 92106426 92106686 92106426 ID=MsG0480023991.01;Name=MsG0480023991.01
Chr4 mRNA 92106426 92106686 92106426 ID=MsG0480023991.01.T01;Parent=MsG0480023991.01;Name=MsG0480023991.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|86
Chr4 exon 92106426 92106686 92106426 ID=MsG0480023991.01.T01:exon:62843;Parent=MsG0480023991.01.T01
Chr4 CDS 92106426 92106686 92106426 ID=MsG0480023991.01.T01:cds;Parent=MsG0480023991.01.T01
Gene Sequence

>MsG0480023991.01.T01

ATGCAGAAGATTGAATCCATGGCAGTTGAGGCATTGAAAATTCAAGCTGACGTGGCCGAAGAAGAAGCTCCATTCGATGTTTCTGCACTCAGCTCAAAGAAAGGGGAAAGTGGGAAAGATCTTTTGGCTTCTGCTATTCCACTTGAGGATTGGATTAGAGACCAAAGCTTAAGCTACAACAAAGGTACTGCAACTGCAAGTTCTAATGGTGAACCTGAAAGAGTCACGCTGATATTGGTTGTCAACTGCGGGATCCAATGA

Protein sequence

>MsG0480023991.01.T01

MQKIESMAVEALKIQADVAEEEAPFDVSALSSKKGESGKDLLASAIPLEDWIRDQSLSYNKGTATASSNGEPERVTLILVVNCGIQ*