AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046905.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046905.01.T01 MTR_4g116870 76.471 102 17 2 12 112 638 733 5.64e-40 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046905.01.T01 AT2G07690 53.774 106 34 2 12 112 630 725 8.72e-25 97.8
MsG0880046905.01.T01 AT2G07690 53.465 101 42 1 12 112 632 727 9.07e-25 97.8

Find 40 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATCTCACGGGCCATTTC+AGG 0.220604 8:-79727143 None:intergenic
AGAATGCCAAGCTTCATTTA+AGG 0.228020 8:+79727861 MsG0880046905.01.T01:three_prime_UTR
GAGAAAGGCGTGTTGTATTT+CGG 0.296469 8:+79727793 MsG0880046905.01.T01:CDS
TGCGTTCTTCGAGTTATTTA+AGG 0.321494 8:+79727893 MsG0880046905.01.T01:three_prime_UTR
TTCTTCTACATGATACCTAA+TGG 0.324040 8:-79727842 None:intergenic
GTTCAGATAAAGAGAAGAAT+CGG 0.335265 8:+79727259 MsG0880046905.01.T01:CDS
CTCTCTGGTGCATGATTAGA+AGG 0.339833 8:-79727752 None:intergenic
GAATGCCAAGCTTCATTTAA+GGG 0.370794 8:+79727862 MsG0880046905.01.T01:three_prime_UTR
GATAAATCTCTCACCTGAAA+TGG 0.405703 8:+79727130 MsG0880046905.01.T01:CDS
ACAATGGATGCAGCTAAGTC+TGG 0.421713 8:+79727095 MsG0880046905.01.T01:CDS
TCAGTCTTCTTTCTGATATG+TGG 0.422676 8:-79727291 None:intergenic
TGAAATGGCCCGTGAGATAC+AGG 0.429731 8:+79727145 MsG0880046905.01.T01:CDS
AAGTAGAGTACAAGCGAGAA+AGG 0.446154 8:+79727778 MsG0880046905.01.T01:CDS
GATGATGATCTCGGCAGAAT+GGG 0.450212 8:+79727312 MsG0880046905.01.T01:CDS
TAAAGAGAAGAATCGGGATT+GGG 0.454048 8:+79727266 MsG0880046905.01.T01:CDS
TCTTCTACATGATACCTAAT+GGG 0.456395 8:-79727841 None:intergenic
AGACACGGTGAAAAGCCTTA+TGG 0.460557 8:-79727072 None:intergenic
TGTACTCTACTTCGTCTCTC+TGG 0.460659 8:-79727767 None:intergenic
TCTAATGTCAATCTTATTGT+GGG 0.462673 8:+79728015 MsG0880046905.01.T01:three_prime_UTR
TGATGATGATCTCGGCAGAA+TGG 0.467139 8:+79727311 MsG0880046905.01.T01:CDS
ATAAAGAGAAGAATCGGGAT+TGG 0.469531 8:+79727265 MsG0880046905.01.T01:CDS
GGTGGGAAAACTGACAAGTT+AGG 0.483332 8:-79727820 None:intergenic
ATCTAATGTCAATCTTATTG+TGG 0.484927 8:+79728014 MsG0880046905.01.T01:three_prime_UTR
CAGGTGAGAGATTTATCTGT+TGG 0.492731 8:-79727124 None:intergenic
GACAAGTGCAAGAAGCCATA+AGG 0.514935 8:+79727057 MsG0880046905.01.T01:CDS
TTCAGATAAAGAGAAGAATC+GGG 0.516845 8:+79727260 MsG0880046905.01.T01:CDS
AATAACTCGAAGAACGCAAT+CGG 0.531133 8:-79727888 None:intergenic
AAGAAGACTGATGATGATCT+CGG 0.570479 8:+79727303 MsG0880046905.01.T01:CDS
CACGACAAACCTGTATCTCA+CGG 0.571503 8:-79727154 None:intergenic
GGATGCCCTTAAATGAAGCT+TGG 0.572015 8:-79727867 None:intergenic
TCTACATGATACCTAATGGG+TGG 0.604704 8:-79727838 None:intergenic
CTACATGATACCTAATGGGT+GGG 0.608345 8:-79727837 None:intergenic
ACGACAAACCTGTATCTCAC+GGG 0.614925 8:-79727153 None:intergenic
AGCTGCATCCATTGTAGACA+CGG 0.626896 8:-79727087 None:intergenic
AAGCTAACCGAGTCTTACAA+AGG 0.627732 8:-79727928 None:intergenic
AACCATGTACTCCAAAACCA+TGG 0.632906 8:+79727985 MsG0880046905.01.T01:three_prime_UTR
TCTCTGGTGCATGATTAGAA+GGG 0.649766 8:-79727751 None:intergenic
CTAATGTCAATCTTATTGTG+GGG 0.655683 8:+79728016 MsG0880046905.01.T01:three_prime_UTR
ATGATGATCTCGGCAGAATG+GGG 0.670531 8:+79727313 MsG0880046905.01.T01:CDS
CCAGATCTAATCATGCTACG+AGG 0.742770 8:+79727035 MsG0880046905.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATGATAAAAAAAAAATC+TGG - Chr8:79726993-79727012 None:intergenic 10.0%
!!! TAAAAGATACACATTTTAAA+CGG + Chr8:79727434-79727453 MsG0880046905.01.T01:intron 15.0%
!! AATTCAAGAGAAGAAAAAAA+AGG - Chr8:79727630-79727649 None:intergenic 20.0%
!! TTCATATATAACAACATTTC+AGG + Chr8:79727719-79727738 MsG0880046905.01.T01:intron 20.0%
!!! AAAAGATACACATTTTAAAC+GGG + Chr8:79727435-79727454 MsG0880046905.01.T01:intron 20.0%
!!! AAATAATACATGTAAGCTTT+TGG - Chr8:79727684-79727703 None:intergenic 20.0%
!!! AATAATACATGTAAGCTTTT+GGG - Chr8:79727683-79727702 None:intergenic 20.0%
!!! TTCTGTAGTCTAAATATTTT+TGG - Chr8:79727519-79727538 None:intergenic 20.0%
!!! TTTTAAAAGTTTGAACCAAA+TGG - Chr8:79727419-79727438 None:intergenic 20.0%
! ATAGCTTATAAACCAGAATT+TGG + Chr8:79727388-79727407 MsG0880046905.01.T01:intron 25.0%
! ATCTAATGTCAATCTTATTG+TGG + Chr8:79728014-79728033 MsG0880046905.01.T01:three_prime_UTR 25.0%
! TCTAATGTCAATCTTATTGT+GGG + Chr8:79728015-79728034 MsG0880046905.01.T01:three_prime_UTR 25.0%
!! AAAGATACACATTTTAAACG+GGG + Chr8:79727436-79727455 MsG0880046905.01.T01:intron 25.0%
!!! TTTTTTTCCTTTGTAAGACT+CGG + Chr8:79727921-79727940 MsG0880046905.01.T01:three_prime_UTR 25.0%
ATCAGCATATGAAACCTATA+TGG - Chr8:79727206-79727225 None:intergenic 30.0%
CTAATGTCAATCTTATTGTG+GGG + Chr8:79728016-79728035 MsG0880046905.01.T01:three_prime_UTR 30.0%
CTGGTAAGAGTTAATTAAAG+AGG - Chr8:79727019-79727038 None:intergenic 30.0%
CTTAAGCAATGCATATTAAG+AGG - Chr8:79726894-79726913 None:intergenic 30.0%
GTTCAGATAAAGAGAAGAAT+CGG + Chr8:79727259-79727278 MsG0880046905.01.T01:CDS 30.0%
TCTTCTACATGATACCTAAT+GGG - Chr8:79727844-79727863 None:intergenic 30.0%
TTAAGAGGATTTACATGCAA+TGG - Chr8:79726879-79726898 None:intergenic 30.0%
TTCAGATAAAGAGAAGAATC+GGG + Chr8:79727260-79727279 MsG0880046905.01.T01:CDS 30.0%
TTCTTCTACATGATACCTAA+TGG - Chr8:79727845-79727864 None:intergenic 30.0%
! ATTTTAAACGGGGTTATGTT+GGG + Chr8:79727446-79727465 MsG0880046905.01.T01:intron 30.0%
! TAGATTCTTTTCCATGGTTT+TGG - Chr8:79727999-79728018 None:intergenic 30.0%
! TGACATTAGATTCTTTTCCA+TGG - Chr8:79728005-79728024 None:intergenic 30.0%
! TTCTCTTTTTCCTTCCATAT+AGG + Chr8:79727189-79727208 MsG0880046905.01.T01:intron 30.0%
!!! AACAGTTTTGAACAGTGTTA+CGG - Chr8:79727584-79727603 None:intergenic 30.0%
AATAACTCGAAGAACGCAAT+CGG - Chr8:79727891-79727910 None:intergenic 35.0%
AGAATGCCAAGCTTCATTTA+AGG + Chr8:79727861-79727880 MsG0880046905.01.T01:three_prime_UTR 35.0%
ATAAAGAGAAGAATCGGGAT+TGG + Chr8:79727265-79727284 MsG0880046905.01.T01:CDS 35.0%
ATCTGGACCAACAAAAAGAT+CGG - Chr8:79726976-79726995 None:intergenic 35.0%
GAATGCCAAGCTTCATTTAA+GGG + Chr8:79727862-79727881 MsG0880046905.01.T01:three_prime_UTR 35.0%
GATAAATCTCTCACCTGAAA+TGG + Chr8:79727130-79727149 MsG0880046905.01.T01:CDS 35.0%
GCATATGAAACCTATATGGA+AGG - Chr8:79727202-79727221 None:intergenic 35.0%
TAAAGAGAAGAATCGGGATT+GGG + Chr8:79727266-79727285 MsG0880046905.01.T01:CDS 35.0%
TCAGTCTTCTTTCTGATATG+TGG - Chr8:79727294-79727313 None:intergenic 35.0%
! AAGAAGACTGATGATGATCT+CGG + Chr8:79727303-79727322 MsG0880046905.01.T01:CDS 35.0%
! CATTTTAAACGGGGTTATGT+TGG + Chr8:79727445-79727464 MsG0880046905.01.T01:intron 35.0%
! TGCGTTCTTCGAGTTATTTA+AGG + Chr8:79727893-79727912 MsG0880046905.01.T01:three_prime_UTR 35.0%
AACCATGTACTCCAAAACCA+TGG + Chr8:79727985-79728004 MsG0880046905.01.T01:three_prime_UTR 40.0%
AAGCTAACCGAGTCTTACAA+AGG - Chr8:79727931-79727950 None:intergenic 40.0%
AAGTAGAGTACAAGCGAGAA+AGG + Chr8:79727778-79727797 MsG0880046905.01.T01:CDS 40.0%
CAGGTGAGAGATTTATCTGT+TGG - Chr8:79727127-79727146 None:intergenic 40.0%
GACCAACAAAAAGATCGGAA+AGG - Chr8:79726971-79726990 None:intergenic 40.0%
GGTGTTTGTGTGCTTATGAT+GGG - Chr8:79727498-79727517 None:intergenic 40.0%
TCTACATGATACCTAATGGG+TGG - Chr8:79727841-79727860 None:intergenic 40.0%
TCTCTGGTGCATGATTAGAA+GGG - Chr8:79727754-79727773 None:intergenic 40.0%
TGACTTTCTTCTCAGCTAAG+TGG - Chr8:79727369-79727388 None:intergenic 40.0%
TGGTGTTTGTGTGCTTATGA+TGG - Chr8:79727499-79727518 None:intergenic 40.0%
! CTACATGATACCTAATGGGT+GGG - Chr8:79727840-79727859 None:intergenic 40.0%
! GAGAAAGGCGTGTTGTATTT+CGG + Chr8:79727793-79727812 MsG0880046905.01.T01:CDS 40.0%
! TGCCTTTCCGATCTTTTTGT+TGG + Chr8:79726966-79726985 MsG0880046905.01.T01:intron 40.0%
!!! TTCCATGGTTTTGGAGTACA+TGG - Chr8:79727990-79728009 None:intergenic 40.0%
ACAATGGATGCAGCTAAGTC+TGG + Chr8:79727095-79727114 MsG0880046905.01.T01:CDS 45.0%
ACATTGTTGCAGCGACAAAG+GGG + Chr8:79727548-79727567 MsG0880046905.01.T01:intron 45.0%
ACGACAAACCTGTATCTCAC+GGG - Chr8:79727156-79727175 None:intergenic 45.0%
AGACACGGTGAAAAGCCTTA+TGG - Chr8:79727075-79727094 None:intergenic 45.0%
AGCTGCATCCATTGTAGACA+CGG - Chr8:79727090-79727109 None:intergenic 45.0%
ATGATGATCTCGGCAGAATG+GGG + Chr8:79727313-79727332 MsG0880046905.01.T01:CDS 45.0%
CAAATGGCGTCACCAAATTC+TGG - Chr8:79727403-79727422 None:intergenic 45.0%
CACATTGTTGCAGCGACAAA+GGG + Chr8:79727547-79727566 MsG0880046905.01.T01:intron 45.0%
CACGACAAACCTGTATCTCA+CGG - Chr8:79727157-79727176 None:intergenic 45.0%
CCAGATCTAATCATGCTACG+AGG + Chr8:79727035-79727054 MsG0880046905.01.T01:intron 45.0%
CCTCGTAGCATGATTAGATC+TGG - Chr8:79727038-79727057 None:intergenic 45.0%
CTCTCTGGTGCATGATTAGA+AGG - Chr8:79727755-79727774 None:intergenic 45.0%
GACAAGTGCAAGAAGCCATA+AGG + Chr8:79727057-79727076 MsG0880046905.01.T01:CDS 45.0%
GATGATGATCTCGGCAGAAT+GGG + Chr8:79727312-79727331 MsG0880046905.01.T01:CDS 45.0%
GGATGCCCTTAAATGAAGCT+TGG - Chr8:79727870-79727889 None:intergenic 45.0%
GGTGGGAAAACTGACAAGTT+AGG - Chr8:79727823-79727842 None:intergenic 45.0%
TCACATTGTTGCAGCGACAA+AGG + Chr8:79727546-79727565 MsG0880046905.01.T01:intron 45.0%
TGTACTCTACTTCGTCTCTC+TGG - Chr8:79727770-79727789 None:intergenic 45.0%
! GCTTTTCACCGTGTCTACAA+TGG + Chr8:79727079-79727098 MsG0880046905.01.T01:CDS 45.0%
! TGATGATGATCTCGGCAGAA+TGG + Chr8:79727311-79727330 MsG0880046905.01.T01:CDS 45.0%
! TGTCAGTTTTCCCACCCATT+AGG + Chr8:79727827-79727846 MsG0880046905.01.T01:three_prime_UTR 45.0%
!! CAGAATTTGGTGACGCCATT+TGG + Chr8:79727401-79727420 MsG0880046905.01.T01:intron 45.0%
TGAAATGGCCCGTGAGATAC+AGG + Chr8:79727145-79727164 MsG0880046905.01.T01:CDS 50.0%
TGTATCTCACGGGCCATTTC+AGG - Chr8:79727146-79727165 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 79726852 79728073 79726852 ID=MsG0880046905.01;Name=MsG0880046905.01
Chr8 mRNA 79726852 79728073 79726852 ID=MsG0880046905.01.T01;Parent=MsG0880046905.01;Name=MsG0880046905.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0.33|0.5|0.75|0|0|4|249|112
Chr8 exon 79726852 79726861 79726852 ID=MsG0880046905.01.T01:exon:34470;Parent=MsG0880046905.01.T01
Chr8 exon 79727039 79727182 79727039 ID=MsG0880046905.01.T01:exon:34471;Parent=MsG0880046905.01.T01
Chr8 exon 79727250 79727350 79727250 ID=MsG0880046905.01.T01:exon:34472;Parent=MsG0880046905.01.T01
Chr8 exon 79727741 79728073 79727741 ID=MsG0880046905.01.T01:exon:34473;Parent=MsG0880046905.01.T01
Chr8 CDS 79726852 79726861 79726852 ID=MsG0880046905.01.T01:cds;Parent=MsG0880046905.01.T01
Chr8 CDS 79727039 79727182 79727039 ID=MsG0880046905.01.T01:cds;Parent=MsG0880046905.01.T01
Chr8 CDS 79727250 79727350 79727250 ID=MsG0880046905.01.T01:cds;Parent=MsG0880046905.01.T01
Chr8 CDS 79727741 79727824 79727741 ID=MsG0880046905.01.T01:cds;Parent=MsG0880046905.01.T01
Chr8 three_prime_UTR 79727825 79728073 79727825 ID=MsG0880046905.01.T01:three_prime_utr;Parent=MsG0880046905.01.T01
Gene Sequence

>MsG0880046905.01.T01

ATGTTTTTGGATCTAATCATGCTACGAGGACAAGTGCAAGAAGCCATAAGGCTTTTCACCGTGTCTACAATGGATGCAGCTAAGTCTGGAATAAACCAACAGATAAATCTCTCACCTGAAATGGCCCGTGAGATACAGGTTTGTCGTGTGATGACAAGCAGAAGTTCAGATAAAGAGAAGAATCGGGATTGGGAACCACATATCAGAAAGAAGACTGATGATGATCTCGGCAGAATGGGGATGAATGAATCTATTGTAAGAAGAGCCCTTCTAATCATGCACCAGAGAGACGAAGTAGAGTACAAGCGAGAAAGGCGTGTTGTATTTCGGAAAGCCTAA

Protein sequence

>MsG0880046905.01.T01

MFLDLIMLRGQVQEAIRLFTVSTMDAAKSGINQQINLSPEMAREIQVCRVMTSRSSDKEKNRDWEPHIRKKTDDDLGRMGMNESIVRRALLIMHQRDEVEYKRERRVVFRKA*