AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007406.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007406.01.T01 MTR_2g024300 78.453 181 21 2 1 171 1 173 2.11e-98 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007406.01.T01 AT4G04330 65.289 121 34 1 50 170 59 171 5.91e-51 161

Find 49 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACTCCCATGTCTTCTTAA+TGG 0.242605 2:+14997209 MsG0280007406.01.T01:three_prime_UTR
CAGATTTGTCCAACCACTTA+TGG 0.272786 2:-14997167 None:intergenic
CTCTTGCCAGCTGGCCAAAA+AGG 0.282297 2:-14995956 None:intergenic
ATATTGATATGTATGGAAAT+TGG 0.302745 2:+14997055 MsG0280007406.01.T01:three_prime_UTR
TGTCCCATTCAAAATCCTTT+TGG 0.366494 2:-14996762 None:intergenic
GACTCCCATGTCTTCTTAAT+GGG 0.375531 2:+14997210 MsG0280007406.01.T01:three_prime_UTR
TGAAGCATATGTTGAGCTAA+TGG 0.380963 2:+14996392 MsG0280007406.01.T01:CDS
TTGTTGGTGCATGGTGTAGC+AGG 0.403399 2:-14995926 None:intergenic
ACTCTAACACGAGGCTTATG+AGG 0.410584 2:+14996930 MsG0280007406.01.T01:CDS
ATTAGCTCAACATATGCTTC+AGG 0.434378 2:-14996390 None:intergenic
AAATCGAGTTAGACCATAAG+TGG 0.434411 2:+14997154 MsG0280007406.01.T01:three_prime_UTR
GTGCAACATATTGATATGTA+TGG 0.445645 2:+14997048 MsG0280007406.01.T01:three_prime_UTR
GAGCTCCTTCTCTTGCCAGC+TGG 0.451237 2:-14995965 None:intergenic
TTGAATAGCCCTACGCATAA+TGG 0.455624 2:+14996633 MsG0280007406.01.T01:intron
CTTCTCAGTTTCAGAACGAC+TGG 0.465661 2:-14996969 None:intergenic
GAAAGGGCTTGCTTTGCTGC+AGG 0.490075 2:-14997000 None:intergenic
TTCGATCAATGGGAATGAAA+GGG 0.495924 2:-14997016 None:intergenic
GTTCGATCAATGGGAATGAA+AGG 0.498668 2:-14997017 None:intergenic
TGTTGAGCTAATGGAATTTG+TGG 0.500110 2:+14996401 MsG0280007406.01.T01:CDS
TACATAGCTCAGTGTTTGCA+TGG 0.509009 2:+14997118 MsG0280007406.01.T01:three_prime_UTR
TACATATCAATATGTTGCAC+AGG 0.515031 2:-14997045 None:intergenic
ACTTGTCACCATCATCTAAC+GGG 0.516413 2:-14996431 None:intergenic
CATTTACCTCCATTATGCGT+AGG 0.520633 2:-14996642 None:intergenic
TCATCAAGACATAAGAACTT+AGG 0.525555 2:+14996477 MsG0280007406.01.T01:CDS
GGTGTAGCAGGGTAAACATA+AGG 0.525917 2:-14995914 None:intergenic
ATTTACCTCCATTATGCGTA+GGG 0.537053 2:-14996641 None:intergenic
ATGTATGGAAATTGGAAAGA+TGG 0.540841 2:+14997063 MsG0280007406.01.T01:three_prime_UTR
TTTGGCCAGCTGGCAAGAGA+AGG 0.542150 2:+14995960 MsG0280007406.01.T01:CDS
AATCGTTGCTGCTCAACTTG+AGG 0.547786 2:+14996204 MsG0280007406.01.T01:CDS
GAGATCAGCATACTGCCAAA+AGG 0.559923 2:+14996747 MsG0280007406.01.T01:CDS
AGAAGCAACACCGGAAGCAA+AGG 0.569743 2:+14996139 MsG0280007406.01.T01:CDS
AACTTGTCACCATCATCTAA+CGG 0.580454 2:-14996432 None:intergenic
TTCTGAAACTGAGAAGTGAA+TGG 0.580741 2:+14996976 MsG0280007406.01.T01:exon
AGTATCCCATTAAGAAGACA+TGG 0.584140 2:-14997215 None:intergenic
TAGAGCTGAAGATGATTCCA+TGG 0.584821 2:-14995876 None:intergenic
CGAGTTAGACCATAAGTGGT+TGG 0.590124 2:+14997158 MsG0280007406.01.T01:three_prime_UTR
CTCTAACACGAGGCTTATGA+GGG 0.595924 2:+14996931 MsG0280007406.01.T01:CDS
TGTTGGTGCATGGTGTAGCA+GGG 0.612247 2:-14995925 None:intergenic
AGGATTCGGAGAAGCAACAC+CGG 0.623746 2:+14996130 MsG0280007406.01.T01:intron
TGGCAGAAGACTCACTCGCA+TGG 0.631217 2:+14997083 MsG0280007406.01.T01:three_prime_UTR
GCTTATGAGGGATTACGTCT+CGG 0.635882 2:+14996943 MsG0280007406.01.T01:CDS
GATAAACACCCGTTAGATGA+TGG 0.636151 2:+14996423 MsG0280007406.01.T01:CDS
TGATGATTCTCTGAACATAG+TGG 0.639406 2:-14996460 None:intergenic
AATAGCCCTACGCATAATGG+AGG 0.664082 2:+14996636 MsG0280007406.01.T01:intron
GCAGTGTAGGTGAAGAATTG+TGG 0.667362 2:-14996175 None:intergenic
GTATCCCATTAAGAAGACAT+GGG 0.697518 2:-14997214 None:intergenic
GGAGCTCTCAATTATCCACA+CGG 0.710162 2:+14995981 MsG0280007406.01.T01:CDS
GTGTAGGTGAAGAATTGTGG+AGG 0.711984 2:-14996172 None:intergenic
TGGTCACTGACTCTAACACG+AGG 0.758847 2:+14996921 MsG0280007406.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGGTGTAATATTATATAT+AGG + Chr2:14996723-14996742 MsG0280007406.01.T01:intron 15.0%
!! AAAACAAACTAAAACAACAA+AGG - Chr2:14996248-14996267 None:intergenic 20.0%
!! ATATTGATATGTATGGAAAT+TGG + Chr2:14997055-14997074 MsG0280007406.01.T01:three_prime_UTR 20.0%
!! TAATAGACACTTAACTATTA+AGG - Chr2:14996529-14996548 None:intergenic 20.0%
! AAAACAACAAAGGCAAATAA+AGG - Chr2:14996238-14996257 None:intergenic 25.0%
! AACAGTTACATGCATTATTA+AGG - Chr2:14996879-14996898 None:intergenic 25.0%
! ACACTTAACTATTAAGGATT+GGG - Chr2:14996523-14996542 None:intergenic 25.0%
! ATACGAAAAATAACACAACT+AGG - Chr2:14996292-14996311 None:intergenic 25.0%
! GGAAGTTGAATAAATTTAGT+AGG - Chr2:14996858-14996877 None:intergenic 25.0%
! TAACCAACAACTATTCTATA+TGG + Chr2:14996551-14996570 MsG0280007406.01.T01:intron 25.0%
! TACGAAAAATAACACAACTA+GGG - Chr2:14996291-14996310 None:intergenic 25.0%
!! AAAACTTGCTTAATAAGTGA+TGG - Chr2:14996691-14996710 None:intergenic 25.0%
!! TTACAACTTCAATTTTGTAC+TGG + Chr2:14996037-14996056 MsG0280007406.01.T01:intron 25.0%
!!! AGTTTTGATTGAGTATACTA+TGG + Chr2:14996705-14996724 MsG0280007406.01.T01:intron 25.0%
AAGACATAAGAACTTAGGTA+AGG + Chr2:14996482-14996501 MsG0280007406.01.T01:intron 30.0%
AATAGTGTGCTATGTTATCT+TGG + Chr2:14996103-14996122 MsG0280007406.01.T01:intron 30.0%
ATGTATGGAAATTGGAAAGA+TGG + Chr2:14997063-14997082 MsG0280007406.01.T01:three_prime_UTR 30.0%
GACACTTAACTATTAAGGAT+TGG - Chr2:14996524-14996543 None:intergenic 30.0%
GTGCAACATATTGATATGTA+TGG + Chr2:14997048-14997067 MsG0280007406.01.T01:three_prime_UTR 30.0%
TACATATCAATATGTTGCAC+AGG - Chr2:14997048-14997067 None:intergenic 30.0%
TCACCATATAGAATAGTTGT+TGG - Chr2:14996557-14996576 None:intergenic 30.0%
TCATCAAGACATAAGAACTT+AGG + Chr2:14996477-14996496 MsG0280007406.01.T01:CDS 30.0%
!! AACTTCAATTTTGTACTGGT+TGG + Chr2:14996041-14996060 MsG0280007406.01.T01:intron 30.0%
AAATCCTGTAAACTGATGTG+TGG + Chr2:14996593-14996612 MsG0280007406.01.T01:intron 35.0%
AAATCGAGTTAGACCATAAG+TGG + Chr2:14997154-14997173 MsG0280007406.01.T01:three_prime_UTR 35.0%
AACTTGTCACCATCATCTAA+CGG - Chr2:14996435-14996454 None:intergenic 35.0%
ACAAAATGTTTGTACCTGGT+CGG + Chr2:14996013-14996032 MsG0280007406.01.T01:intron 35.0%
ACAACAAAGGCAAATAAAGG+AGG - Chr2:14996235-14996254 None:intergenic 35.0%
AGTATCCCATTAAGAAGACA+TGG - Chr2:14997218-14997237 None:intergenic 35.0%
ATGCACAAAATGTTTGTACC+TGG + Chr2:14996009-14996028 MsG0280007406.01.T01:intron 35.0%
ATTAGCTCAACATATGCTTC+AGG - Chr2:14996393-14996412 None:intergenic 35.0%
ATTTACCTCCATTATGCGTA+GGG - Chr2:14996644-14996663 None:intergenic 35.0%
GTATCCCATTAAGAAGACAT+GGG - Chr2:14997217-14997236 None:intergenic 35.0%
TGAAGCATATGTTGAGCTAA+TGG + Chr2:14996392-14996411 MsG0280007406.01.T01:CDS 35.0%
TGATGATTCTCTGAACATAG+TGG - Chr2:14996463-14996482 None:intergenic 35.0%
TGTTGAGCTAATGGAATTTG+TGG + Chr2:14996401-14996420 MsG0280007406.01.T01:CDS 35.0%
TTCGATCAATGGGAATGAAA+GGG - Chr2:14997019-14997038 None:intergenic 35.0%
! ACTGCCAAAAGGATTTTGAA+TGG + Chr2:14996758-14996777 MsG0280007406.01.T01:CDS 35.0%
! CCAAAAAGGATAGCTTTTGT+TGG - Chr2:14995945-14995964 None:intergenic 35.0%
! CCAACAAAAGCTATCCTTTT+TGG + Chr2:14995942-14995961 MsG0280007406.01.T01:CDS 35.0%
! CTGCCAAAAGGATTTTGAAT+GGG + Chr2:14996759-14996778 MsG0280007406.01.T01:CDS 35.0%
! TGAAGAATTGTGGAGGTTTT+TGG - Chr2:14996168-14996187 None:intergenic 35.0%
! TGTCCCATTCAAAATCCTTT+TGG - Chr2:14996765-14996784 None:intergenic 35.0%
!! TTCTGAAACTGAGAAGTGAA+TGG + Chr2:14996976-14996995 MsG0280007406.01.T01:exon 35.0%
!!! AACGATTTTAACTGCAGTGT+AGG - Chr2:14996191-14996210 None:intergenic 35.0%
!!! CTTGAAGCGTTTAGCTTTTA+AGG + Chr2:14996786-14996805 MsG0280007406.01.T01:CDS 35.0%
ACTTGTCACCATCATCTAAC+GGG - Chr2:14996434-14996453 None:intergenic 40.0%
ATTGAAGTTGTAAACCGACC+AGG - Chr2:14996030-14996049 None:intergenic 40.0%
CAGATTTGTCCAACCACTTA+TGG - Chr2:14997170-14997189 None:intergenic 40.0%
CAGGATTATAACTCTGCTCA+TGG - Chr2:14996374-14996393 None:intergenic 40.0%
CATTTACCTCCATTATGCGT+AGG - Chr2:14996645-14996664 None:intergenic 40.0%
GACTCCCATGTCTTCTTAAT+GGG + Chr2:14997210-14997229 MsG0280007406.01.T01:three_prime_UTR 40.0%
GAGGTAAATGGATGACTAGT+TGG + Chr2:14996655-14996674 MsG0280007406.01.T01:intron 40.0%
GATAAACACCCGTTAGATGA+TGG + Chr2:14996423-14996442 MsG0280007406.01.T01:CDS 40.0%
GTTATCTTGGATGCAGGATT+CGG + Chr2:14996116-14996135 MsG0280007406.01.T01:intron 40.0%
GTTCGATCAATGGGAATGAA+AGG - Chr2:14997020-14997039 None:intergenic 40.0%
TACATAGCTCAGTGTTTGCA+TGG + Chr2:14997118-14997137 MsG0280007406.01.T01:three_prime_UTR 40.0%
TGCTATGTTATCTTGGATGC+AGG + Chr2:14996110-14996129 MsG0280007406.01.T01:intron 40.0%
TTGAATAGCCCTACGCATAA+TGG + Chr2:14996633-14996652 MsG0280007406.01.T01:intron 40.0%
! ACAGGCTTTTGTTCGATCAA+TGG - Chr2:14997030-14997049 None:intergenic 40.0%
! CAGGCTTTTGTTCGATCAAT+GGG - Chr2:14997029-14997048 None:intergenic 40.0%
! CTACGCATAATGGAGGTAAA+TGG + Chr2:14996643-14996662 MsG0280007406.01.T01:intron 40.0%
AATAGCCCTACGCATAATGG+AGG + Chr2:14996636-14996655 MsG0280007406.01.T01:intron 45.0%
AATCGTTGCTGCTCAACTTG+AGG + Chr2:14996204-14996223 MsG0280007406.01.T01:CDS 45.0%
ACTCTAACACGAGGCTTATG+AGG + Chr2:14996930-14996949 MsG0280007406.01.T01:CDS 45.0%
AGAGCCACACATCAGTTTAC+AGG - Chr2:14996600-14996619 None:intergenic 45.0%
AGTCAGTGACCATCTACAAG+AGG - Chr2:14996913-14996932 None:intergenic 45.0%
CGACTCCCATGTCTTCTTAA+TGG + Chr2:14997209-14997228 MsG0280007406.01.T01:three_prime_UTR 45.0%
CGAGTTAGACCATAAGTGGT+TGG + Chr2:14997158-14997177 MsG0280007406.01.T01:three_prime_UTR 45.0%
CTCTAACACGAGGCTTATGA+GGG + Chr2:14996931-14996950 MsG0280007406.01.T01:CDS 45.0%
CTTCTCAGTTTCAGAACGAC+TGG - Chr2:14996972-14996991 None:intergenic 45.0%
GAGATCAGCATACTGCCAAA+AGG + Chr2:14996747-14996766 MsG0280007406.01.T01:CDS 45.0%
GCAGTGTAGGTGAAGAATTG+TGG - Chr2:14996178-14996197 None:intergenic 45.0%
GCTTATGAGGGATTACGTCT+CGG + Chr2:14996943-14996962 MsG0280007406.01.T01:CDS 45.0%
GGAGCTCTCAATTATCCACA+CGG + Chr2:14995981-14996000 MsG0280007406.01.T01:CDS 45.0%
GGTGTAGCAGGGTAAACATA+AGG - Chr2:14995917-14995936 None:intergenic 45.0%
GTGTAGGTGAAGAATTGTGG+AGG - Chr2:14996175-14996194 None:intergenic 45.0%
TAGCGTTGTCCTCTTGTAGA+TGG + Chr2:14996901-14996920 MsG0280007406.01.T01:intron 45.0%
! GGATAGCTTTTGTTGGTGCA+TGG - Chr2:14995938-14995957 None:intergenic 45.0%
! TTTTGTGCATTGCAACCGTG+TGG - Chr2:14995999-14996018 None:intergenic 45.0%
!! TTTTTGGCAGCCTTTGCTTC+CGG - Chr2:14996152-14996171 None:intergenic 45.0%
AGAAGCAACACCGGAAGCAA+AGG + Chr2:14996139-14996158 MsG0280007406.01.T01:CDS 50.0%
AGGATTCGGAGAAGCAACAC+CGG + Chr2:14996130-14996149 MsG0280007406.01.T01:intron 50.0%
TGTTGGTGCATGGTGTAGCA+GGG - Chr2:14995928-14995947 None:intergenic 50.0%
TTGTTGGTGCATGGTGTAGC+AGG - Chr2:14995929-14995948 None:intergenic 50.0%
!! ACTGATGTGTGGCTCTGACA+TGG + Chr2:14996604-14996623 MsG0280007406.01.T01:intron 50.0%
!! AGCTATCCTTTTTGGCCAGC+TGG + Chr2:14995950-14995969 MsG0280007406.01.T01:CDS 50.0%
!! TGGTCACTGACTCTAACACG+AGG + Chr2:14996921-14996940 MsG0280007406.01.T01:CDS 50.0%
CTCTTGCCAGCTGGCCAAAA+AGG - Chr2:14995959-14995978 None:intergenic 55.0%
TGGCAGAAGACTCACTCGCA+TGG + Chr2:14997083-14997102 MsG0280007406.01.T01:three_prime_UTR 55.0%
TTTGGCCAGCTGGCAAGAGA+AGG + Chr2:14995960-14995979 MsG0280007406.01.T01:CDS 55.0%
! GAAAGGGCTTGCTTTGCTGC+AGG - Chr2:14997003-14997022 None:intergenic 55.0%
GAGCTCCTTCTCTTGCCAGC+TGG - Chr2:14995968-14995987 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 14995878 14997291 14995878 ID=MsG0280007406.01;Name=MsG0280007406.01
Chr2 mRNA 14995878 14997291 14995878 ID=MsG0280007406.01.T01;Parent=MsG0280007406.01;Name=MsG0280007406.01.T01;_AED=0.27;_eAED=0.28;_QI=0|0.6|0.66|1|0|0|6|297|171
Chr2 exon 14995878 14996002 14995878 ID=MsG0280007406.01.T01:exon:3187;Parent=MsG0280007406.01.T01
Chr2 exon 14996132 14996225 14996132 ID=MsG0280007406.01.T01:exon:3188;Parent=MsG0280007406.01.T01
Chr2 exon 14996381 14996498 14996381 ID=MsG0280007406.01.T01:exon:3189;Parent=MsG0280007406.01.T01
Chr2 exon 14996641 14996657 14996641 ID=MsG0280007406.01.T01:exon:3190;Parent=MsG0280007406.01.T01
Chr2 exon 14996745 14996831 14996745 ID=MsG0280007406.01.T01:exon:3191;Parent=MsG0280007406.01.T01
Chr2 exon 14996920 14997291 14996920 ID=MsG0280007406.01.T01:exon:3192;Parent=MsG0280007406.01.T01
Chr2 CDS 14995878 14996002 14995878 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 CDS 14996132 14996225 14996132 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 CDS 14996381 14996498 14996381 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 CDS 14996641 14996657 14996641 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 CDS 14996745 14996831 14996745 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 CDS 14996920 14996994 14996920 ID=MsG0280007406.01.T01:cds;Parent=MsG0280007406.01.T01
Chr2 three_prime_UTR 14996995 14997291 14996995 ID=MsG0280007406.01.T01:three_prime_utr;Parent=MsG0280007406.01.T01
Gene Sequence

>MsG0280007406.01.T01

ATGGAATCATCTTCAGCTCTAACACTTTCTCAGCTTCCTTATGTTTACCCTGCTACACCATGCACCAACAAAAGCTATCCTTTTTGGCCAGCTGGCAAGAGAAGGAGCTCTCAATTATCCACACGGATTCGGAGAAGCAACACCGGAAGCAAAGGCTGCCAAAAACCTCCACAATTCTTCACCTACACTGCAGTTAAAATCGTTGCTGCTCAACTTGAGAGTTATAATCCTGAAGCATATGTTGAGCTAATGGAATTTGTGGATAAACACCCGTTAGATGATGGTGACAAGTTCTGCGCCACTATGTTCAGAGAATCATCAAGACATAAGAACTTAGCCCTACGCATAATGGAGGTGAGATCAGCATACTGCCAAAAGGATTTTGAATGGGACAACTTGAAGCGTTTAGCTTTTAAGGTTTGTAATTCAATAGCTTTCTGTATGGTCACTGACTCTAACACGAGGCTTATGAGGGATTACGTCTCGGAAACCAGTCGTTCTGAAACTGAGAAGTGA

Protein sequence

>MsG0280007406.01.T01

MESSSALTLSQLPYVYPATPCTNKSYPFWPAGKRRSSQLSTRIRRSNTGSKGCQKPPQFFTYTAVKIVAAQLESYNPEAYVELMEFVDKHPLDDGDKFCATMFRESSRHKNLALRIMEVRSAYCQKDFEWDNLKRLAFKVCNSIAFCMVTDSNTRLMRDYVSETSRSETEK*