AlfalfaGEDB | MsG0280010140.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010140.01

KEGG: K05577
GO: -
PFAM: PF00361

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010140.01.T01	RHN44673.1	96.053	76	3	0	1	76	274	349	8.57E-39	141

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010140.01.T01	Q32905	90.625	64	6	0	1	64	18	81	4.49E-31	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010140.01.T01	A0A396GVX7	96.053	76	3	0	1	76	274	349	4.09e-39	141

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048690.01	MsG0280010140.01	0.819973	8.764619e-53	6.846510e-50
MsG0080048691.01	MsG0280010140.01	0.814638	1.391605e-51	9.388230e-49
MsG0080048692.01	MsG0280010140.01	0.828149	1.055755e-54	1.040614e-51
MsG0080048693.01	MsG0280010140.01	0.830607	2.670348e-55	2.827367e-52
MsG0080048694.01	MsG0280010140.01	0.808812	2.577143e-50	1.487976e-47
MsG0080048695.01	MsG0280010140.01	0.808851	2.527172e-50	1.460662e-47
MsG0080048697.01	MsG0280010140.01	0.812835	3.471245e-51	2.230092e-48
MsG0080048699.01	MsG0280010140.01	0.807297	5.415492e-50	3.005336e-47
MsG0080049076.01	MsG0280010140.01	0.824763	6.766145e-54	6.050218e-51
MsG0280010140.01	MsG0280010142.01	0.802301	5.987027e-49	2.922668e-46
MsG0280010140.01	MsG0280010145.01	0.805573	1.250767e-49	6.640008e-47
MsG0280010140.01	MsG0380013448.01	0.802220	6.220152e-49	3.030018e-46
MsG0280010140.01	MsG0380013888.01	0.850885	1.271061e-60	2.526519e-57
MsG0280010140.01	MsG0380013921.01	0.817081	3.967660e-52	2.861336e-49
MsG0280010140.01	MsG0380013926.01	0.852093	5.788969e-61	1.196463e-57
MsG0280010140.01	MsG0380013928.01	0.804560	2.037287e-49	1.053719e-46
MsG0280010140.01	MsG0380013929.01	0.812273	4.605336e-51	2.915360e-48
MsG0280010140.01	MsG0380013931.01	0.823416	1.401383e-53	1.205774e-50
MsG0280010140.01	MsG0380013940.01	0.830665	2.584695e-55	2.741348e-52
MsG0280010140.01	MsG0380013941.01	0.855470	6.175341e-62	1.427292e-58
MsG0280010140.01	MsG0380013942.01	0.811767	5.938047e-51	3.707700e-48
MsG0280010140.01	MsG0380013943.01	0.821258	4.442099e-53	3.595706e-50
MsG0280010140.01	MsG0380013944.01	0.829817	4.162630e-55	4.308572e-52
MsG0280010140.01	MsG0380013945.01	0.837832	4.119679e-57	5.417023e-54
MsG0280010140.01	MsG0380013951.01	0.822347	2.486577e-53	2.075975e-50
MsG0280010140.01	MsG0380013953.01	0.829119	6.152847e-55	6.238469e-52
MsG0280010140.01	MsG0380013956.01	0.817769	2.776617e-52	2.040607e-49
MsG0280010140.01	MsG0380013957.01	0.829504	4.961591e-55	5.087627e-52
MsG0280010140.01	MsG0380013961.01	0.802671	5.023025e-49	2.475139e-46
MsG0280010140.01	MsG0380013962.01	0.801340	9.429599e-49	4.492154e-46
MsG0280010140.01	MsG0380013966.01	0.859638	3.600880e-63	9.575652e-60
MsG0280010140.01	MsG0380013967.01	0.831520	1.593224e-55	1.733210e-52
MsG0280010140.01	MsG0380013968.01	0.820439	6.856783e-53	5.425585e-50
MsG0280010140.01	MsG0380013971.01	0.833587	4.896465e-56	5.663916e-53
MsG0280010140.01	MsG0380013975.01	0.806284	8.863579e-50	4.792076e-47
MsG0280010140.01	MsG0380013977.01	0.833765	4.418384e-56	5.138584e-53
MsG0280010140.01	MsG0380013999.01	0.812065	5.114229e-51	3.218944e-48
MsG0280010140.01	MsG0380014004.01	0.828048	1.116035e-54	1.096800e-51
MsG0280010140.01	MsG0380014005.01	0.842023	3.329829e-58	4.983026e-55
MsG0280010140.01	MsG0380014006.01	0.819684	1.020356e-52	7.905867e-50
MsG0280010140.01	MsG0380014008.01	0.813930	1.994445e-51	1.319901e-48
MsG0280010140.01	MsG0380014017.01	0.818672	1.734367e-52	1.306671e-49
MsG0280010140.01	MsG0380014020.01	0.832445	9.415552e-56	1.052592e-52
MsG0280010140.01	MsG0380014021.01	0.812314	4.513301e-51	2.860129e-48
MsG0280010140.01	MsG0380014023.01	0.817722	2.846217e-52	2.088987e-49
MsG0280010140.01	MsG0380014024.01	0.802533	5.363384e-49	2.633555e-46
MsG0280010140.01	MsG0380014026.01	0.854122	1.518996e-61	3.357945e-58
MsG0280010140.01	MsG0380014029.01	0.804275	2.335725e-49	1.199256e-46
MsG0280010140.01	MsG0380014032.01	0.843723	1.175727e-58	1.856094e-55
MsG0280010140.01	MsG0380014033.01	0.840253	9.721906e-58	1.376926e-54
MsG0280010140.01	MsG0380014039.01	0.822612	2.158253e-53	1.815671e-50
MsG0280010140.01	MsG0380014040.01	0.831528	1.586523e-55	1.726324e-52
MsG0280010140.01	MsG0380017007.01	0.821888	3.177976e-53	2.618584e-50
MsG0280010140.01	MsG0480018296.01	0.822111	2.820687e-53	2.338920e-50
MsG0280010140.01	MsG0580027677.01	0.831877	1.300763e-55	1.430240e-52
MsG0280010140.01	MsG0780038197.01	0.823052	1.704203e-53	1.451602e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010140.01.T01	MTR_4g006150	95.312	64	3	1	64	254	317	2.74e-32	114
MsG0280010140.01.T01	MTR_7g021740	95.312	64	3	1	64	299	362	3.71e-30	111
MsG0280010140.01.T01	MTR_4g051300	95.312	64	3	1	64	254	317	9.24e-30	110
MsG0280010140.01.T01	MTR_0002s0200	75.000	64	16	1	64	171	234	2.22e-25	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010140.01.T01	ATCG01010	84.375	64	10	0	1	64	254	317	6.40e-27	102

Find 17 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAAGCTATTACACTCATTA+TGG	0.154583	2:-65293665	None:intergenic
CTATTCTACAATGTCTCAAT+TGG	0.311547	2:+65293758	MsG0280010140.01.T01:CDS
TCTTATCCATGCTGCTACTA+TGG	0.335623	2:+65293585	MsG0280010140.01.T01:CDS
GGTATAATAACAGTAGTATT+AGG	0.362110	2:+65293691	MsG0280010140.01.T01:CDS
GCATGGATAAGAGCGGAAAT+AGG	0.395842	2:-65293574	None:intergenic
AAAGCTATTACACTCATTAT+GGG	0.397679	2:-65293664	None:intergenic
CAATTGAGACATTGTAGAAT+AGG	0.439014	2:-65293757	None:intergenic
ATGAGTGTAATAGCTTTGAT+AGG	0.464349	2:+65293670	MsG0280010140.01.T01:CDS
TATTCTACAATGTCTCAATT+GGG	0.475782	2:+65293759	MsG0280010140.01.T01:CDS
AAGCTATTACACTCATTATG+GGG	0.579025	2:-65293663	None:intergenic
GCTGCTACTATGGTAGCAGC+AGG	0.579811	2:+65293595	MsG0280010140.01.T01:CDS
AGCTATTACACTCATTATGG+GGG	0.611408	2:-65293662	None:intergenic
CTGCTACCATAGTAGCAGCA+TGG	0.613737	2:-65293591	None:intergenic
ATAAGAGCGGAAATAGGAGT+AGG	0.645302	2:-65293568	None:intergenic
TGGTTACCAGATGCTATGGA+AGG	0.647532	2:+65293544	None:intergenic
GCTATTACACTCATTATGGG+GGG	0.682426	2:-65293661	None:intergenic
AGTAGCAGCATGGATAAGAG+CGG	0.698547	2:-65293581	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAGATATTAAGAAAAATT+TGG	+	Chr2:65293734-65293753	MsG0280010140.01.T01:CDS	10.0%
!	AAAGCTATTACACTCATTAT+GGG	-	Chr2:65293667-65293686	None:intergenic	25.0%
!	GGTATAATAACAGTAGTATT+AGG	+	Chr2:65293691-65293710	MsG0280010140.01.T01:CDS	25.0%
!	TATTCTACAATGTCTCAATT+GGG	+	Chr2:65293759-65293778	MsG0280010140.01.T01:CDS	25.0%
	AAGCTATTACACTCATTATG+GGG	-	Chr2:65293666-65293685	None:intergenic	30.0%
	CAAAGCTATTACACTCATTA+TGG	-	Chr2:65293668-65293687	None:intergenic	30.0%
	CAATTGAGACATTGTAGAAT+AGG	-	Chr2:65293760-65293779	None:intergenic	30.0%
	CTATTCTACAATGTCTCAAT+TGG	+	Chr2:65293758-65293777	MsG0280010140.01.T01:CDS	30.0%
!!	ATGAGTGTAATAGCTTTGAT+AGG	+	Chr2:65293670-65293689	MsG0280010140.01.T01:CDS	30.0%
	AGCTATTACACTCATTATGG+GGG	-	Chr2:65293665-65293684	None:intergenic	35.0%
	ATAAGAGCGGAAATAGGAGT+AGG	-	Chr2:65293571-65293590	None:intergenic	40.0%
	GCTATTACACTCATTATGGG+GGG	-	Chr2:65293664-65293683	None:intergenic	40.0%
	TCTTATCCATGCTGCTACTA+TGG	+	Chr2:65293585-65293604	MsG0280010140.01.T01:CDS	40.0%
!	GGGGGTATAACTATGAAAAG+AGG	-	Chr2:65293646-65293665	None:intergenic	40.0%
	AGTAGCAGCATGGATAAGAG+CGG	-	Chr2:65293584-65293603	None:intergenic	45.0%
	GCATGGATAAGAGCGGAAAT+AGG	-	Chr2:65293577-65293596	None:intergenic	45.0%
	CTGCTACCATAGTAGCAGCA+TGG	-	Chr2:65293594-65293613	None:intergenic	50.0%
	GCTGCTACTATGGTAGCAGC+AGG	+	Chr2:65293595-65293614	MsG0280010140.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65293559	65293789	65293559	ID=MsG0280010140.01;Name=MsG0280010140.01
Chr2	mRNA	65293559	65293789	65293559	ID=MsG0280010140.01.T01;Parent=MsG0280010140.01;Name=MsG0280010140.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|76
Chr2	exon	65293559	65293789	65293559	ID=MsG0280010140.01.T01:exon:18062;Parent=MsG0280010140.01.T01
Chr2	CDS	65293559	65293789	65293559	ID=MsG0280010140.01.T01:cds;Parent=MsG0280010140.01.T01

Gene Sequence	>MsG0280010140.01.T01 ATGGAAGGTCCTACTCCTATTTCCGCTCTTATCCATGCTGCTACTATGGTAGCAGCAGGAATTTTTCTTGTAGCTCGACTTCTTCCTCTTTTCATAGTTATACCCCCCATAATGAGTGTAATAGCTTTGATAGGTATAATAACAGTAGTATTAGGAGCTACTTTAGCGATTGCTCAAAAGATATTAAGAAAAATTTGGCCTATTCTACAATGTCTCAATTGGGTTATATGA
Protein sequence	>MsG0280010140.01.T01 MEGPTPISALIHAATMVAAGIFLVARLLPLFIVIPPIMSVIALIGIITVVLGATLAIAQKILRKIWPILQCLNWVI*