AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037453.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037453.01.T01 MTR_7g033325 66.879 157 1 1 33 138 205 361 4.52e-63 198
MsG0780037453.01.T01 MTR_7g033325 66.456 158 2 1 33 139 206 363 4.63e-63 198
MsG0780037453.01.T01 MTR_8g089840 59.873 157 12 1 33 138 211 367 2.69e-56 181
MsG0780037453.01.T01 MTR_3g096150 57.325 157 16 1 33 138 186 342 3.16e-54 174
MsG0780037453.01.T01 MTR_4g115070 45.513 156 35 1 33 138 183 338 4.54e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037453.01.T01 AT3G27540 58.599 157 14 1 33 138 205 361 3.22e-55 178
MsG0780037453.01.T01 AT5G14480 59.236 157 13 1 33 138 206 362 4.08e-54 175
MsG0780037453.01.T01 AT1G67880 57.595 158 16 1 33 139 209 366 1.97e-53 173
MsG0780037453.01.T01 AT3G01620 56.875 160 18 1 33 141 207 366 1.03e-52 171
MsG0780037453.01.T01 AT1G12990 56.688 157 17 1 33 138 211 367 6.85e-52 169
MsG0780037453.01.T01 AT2G13290 41.250 160 43 1 30 138 178 337 7.19e-34 122
MsG0780037453.01.T01 AT2G13290 41.250 160 43 1 30 138 178 337 7.19e-34 122
MsG0780037453.01.T01 AT3G26445 50.746 67 24 2 37 94 48 114 2.50e-14 65.5

Find 34 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGAAGAGTATACATTTA+AGG 0.167482 7:-26164682 MsG0780037453.01.T01:CDS
GAACAATCGGTGGAAGATTC+AGG 0.321978 7:-26165174 MsG0780037453.01.T01:CDS
TCCTTAAATGTATACTCTTC+AGG 0.352191 7:+26164681 None:intergenic
TGACGTGCCTCCTCGATAAA+CGG 0.388943 7:+26165139 None:intergenic
TGCATATCACGTTCTGAATC+CGG 0.391551 7:+26164730 None:intergenic
TATACATTTAAGGAGATTAT+CGG 0.397622 7:-26164672 MsG0780037453.01.T01:CDS
ATCACGTTCTGAATCCGGTC+AGG 0.441353 7:+26164735 None:intergenic
CTCATTACTTAAACCCTGAC+CGG 0.448929 7:-26164749 MsG0780037453.01.T01:CDS
CTCAGTTGTAGATGAAGAAT+CGG 0.476003 7:+26164988 None:intergenic
AAGGAGATTATCGGAAAACT+AGG 0.496659 7:-26164663 MsG0780037453.01.T01:CDS
TATTCAGCAGTGCATTTCCT+CGG 0.498967 7:-26164624 MsG0780037453.01.T01:CDS
CGGTCAGGGTTTAAGTAATG+AGG 0.504563 7:+26164750 None:intergenic
CGACTAACATATGGAACAAT+CGG 0.508701 7:-26165187 None:intergenic
GATCAGCTTCTGAGGATAGC+AGG 0.518822 7:-26165104 MsG0780037453.01.T01:CDS
CGGTGGAAGATTCAGGAGAA+AGG 0.526990 7:-26165167 MsG0780037453.01.T01:CDS
GCTGAATAAGAATGAGGAAT+CGG 0.531188 7:+26164639 None:intergenic
GAGATTGATTGTGTGTGCAC+TGG 0.534267 7:+26165035 None:intergenic
TCACGTTCTGAATCCGGTCA+GGG 0.579215 7:+26164736 None:intergenic
AGATTGATTGTGTGTGCACT+GGG 0.593842 7:+26165036 None:intergenic
TGCACTGCTGAATAAGAATG+AGG 0.596632 7:+26164633 None:intergenic
GTTGTGTGGATCAGCTTCTG+AGG 0.602029 7:-26165112 MsG0780037453.01.T01:CDS
CGATTCTTCATCTACAACTG+AGG 0.609395 7:-26164987 MsG0780037453.01.T01:CDS
CACAAATGCAGAAGATGCCG+AGG 0.622278 7:+26164607 None:intergenic
GGAATCGCACTCGATCATAG+TGG 0.631798 7:+26164772 None:intergenic
ATTCAGAACGTGATATGCAA+AGG 0.632732 7:-26164726 MsG0780037453.01.T01:CDS
GGTCAGGGTTTAAGTAATGA+GGG 0.637006 7:+26164751 None:intergenic
AGTTGTAGATGAAGAATCGG+AGG 0.638652 7:+26164991 None:intergenic
GTTGTAGATGAAGAATCGGA+GGG 0.639453 7:+26164992 None:intergenic
CACACACAATCAATCTCTTG+AGG 0.645085 7:-26165029 MsG0780037453.01.T01:CDS
GGAAAATCCGTTTATCGAGG+AGG 0.661999 7:-26165146 MsG0780037453.01.T01:CDS
ACACAATCAATCTCTTGAGG+TGG 0.662731 7:-26165026 MsG0780037453.01.T01:CDS
AGGCACGTCAAAGAGTTGTG+TGG 0.666339 7:-26165126 MsG0780037453.01.T01:CDS
CTAACATATGGAACAATCGG+TGG 0.687645 7:-26165184 None:intergenic
AAAGGAAAATCCGTTTATCG+AGG 0.694930 7:-26165149 MsG0780037453.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACATTTAAGGAGATTAT+CGG - Chr7:26165104-26165123 MsG0780037453.01.T01:CDS 20.0%
! ATACAAATTCACTGATATGA+CGG + Chr7:26164965-26164984 None:intergenic 25.0%
TCCTTAAATGTATACTCTTC+AGG + Chr7:26165098-26165117 None:intergenic 30.0%
! TCCTGAAGAGTATACATTTA+AGG - Chr7:26165094-26165113 MsG0780037453.01.T01:CDS 30.0%
! TTTGTTGTCAAGGAAAAACT+CGG + Chr7:26164832-26164851 None:intergenic 30.0%
!!! TCTTTTGATAATGTCTGATG+TGG - Chr7:26164709-26164728 MsG0780037453.01.T01:CDS 30.0%
AAAGGAAAATCCGTTTATCG+AGG - Chr7:26164627-26164646 MsG0780037453.01.T01:CDS 35.0%
AAGGAGATTATCGGAAAACT+AGG - Chr7:26165113-26165132 MsG0780037453.01.T01:CDS 35.0%
ATTCAGAACGTGATATGCAA+AGG - Chr7:26165050-26165069 MsG0780037453.01.T01:CDS 35.0%
CTCAGTTGTAGATGAAGAAT+CGG + Chr7:26164791-26164810 None:intergenic 35.0%
GCTGAATAAGAATGAGGAAT+CGG + Chr7:26165140-26165159 None:intergenic 35.0%
! TTTTCCTTGACAACAAAAGC+TGG - Chr7:26164835-26164854 MsG0780037453.01.T01:intron 35.0%
ACACAATCAATCTCTTGAGG+TGG - Chr7:26164750-26164769 MsG0780037453.01.T01:CDS 40.0%
AGATTGATTGTGTGTGCACT+GGG + Chr7:26164743-26164762 None:intergenic 40.0%
AGTTGTAGATGAAGAATCGG+AGG + Chr7:26164788-26164807 None:intergenic 40.0%
CACACACAATCAATCTCTTG+AGG - Chr7:26164747-26164766 MsG0780037453.01.T01:CDS 40.0%
CGATTCTTCATCTACAACTG+AGG - Chr7:26164789-26164808 MsG0780037453.01.T01:CDS 40.0%
CTCATTACTTAAACCCTGAC+CGG - Chr7:26165027-26165046 MsG0780037453.01.T01:CDS 40.0%
GGTCAGGGTTTAAGTAATGA+GGG + Chr7:26165028-26165047 None:intergenic 40.0%
GTTGTAGATGAAGAATCGGA+GGG + Chr7:26164787-26164806 None:intergenic 40.0%
TATTCAGCAGTGCATTTCCT+CGG - Chr7:26165152-26165171 MsG0780037453.01.T01:CDS 40.0%
TGCACTGCTGAATAAGAATG+AGG + Chr7:26165146-26165165 None:intergenic 40.0%
TTGACAACAAAAGCTGGAGA+GGG - Chr7:26164841-26164860 MsG0780037453.01.T01:intron 40.0%
! TGCATATCACGTTCTGAATC+CGG + Chr7:26165049-26165068 None:intergenic 40.0%
ACAGTCTGATCTACTGCTGT+CGG - Chr7:26164911-26164930 MsG0780037453.01.T01:intron 45.0%
CGGTCAGGGTTTAAGTAATG+AGG + Chr7:26165029-26165048 None:intergenic 45.0%
CTTGACAACAAAAGCTGGAG+AGG - Chr7:26164840-26164859 MsG0780037453.01.T01:intron 45.0%
GAACAATCGGTGGAAGATTC+AGG - Chr7:26164602-26164621 MsG0780037453.01.T01:CDS 45.0%
GAGATTGATTGTGTGTGCAC+TGG + Chr7:26164744-26164763 None:intergenic 45.0%
GGAAAATCCGTTTATCGAGG+AGG - Chr7:26164630-26164649 MsG0780037453.01.T01:CDS 45.0%
GTGTGCATACCTTGTCTTGT+CGG + Chr7:26164886-26164905 None:intergenic 45.0%
TCTTGTCGGTCTGGTATCTA+TGG + Chr7:26164872-26164891 None:intergenic 45.0%
!! CTCTCCAGCTTTTGTTGTCA+AGG + Chr7:26164842-26164861 None:intergenic 45.0%
AGGCACGTCAAAGAGTTGTG+TGG - Chr7:26164650-26164669 MsG0780037453.01.T01:CDS 50.0%
CACAAATGCAGAAGATGCCG+AGG + Chr7:26165172-26165191 None:intergenic 50.0%
CATACCTTGTCTTGTCGGTC+TGG + Chr7:26164881-26164900 None:intergenic 50.0%
CGGTGGAAGATTCAGGAGAA+AGG - Chr7:26164609-26164628 MsG0780037453.01.T01:CDS 50.0%
GATACCAGACCGACAAGACA+AGG - Chr7:26164874-26164893 MsG0780037453.01.T01:intron 50.0%
GGAATCGCACTCGATCATAG+TGG + Chr7:26165007-26165026 None:intergenic 50.0%
GTTGTGTGGATCAGCTTCTG+AGG - Chr7:26164664-26164683 MsG0780037453.01.T01:CDS 50.0%
TGACGTGCCTCCTCGATAAA+CGG + Chr7:26164640-26164659 None:intergenic 50.0%
! ATCACGTTCTGAATCCGGTC+AGG + Chr7:26165044-26165063 None:intergenic 50.0%
! GATCAGCTTCTGAGGATAGC+AGG - Chr7:26164672-26164691 MsG0780037453.01.T01:CDS 50.0%
! TCACGTTCTGAATCCGGTCA+GGG + Chr7:26165043-26165062 None:intergenic 50.0%
GATCTACTGCTGTCGGATGC+TGG - Chr7:26164918-26164937 MsG0780037453.01.T01:intron 55.0%
TACTGCTGTCGGATGCTGGC+TGG - Chr7:26164922-26164941 MsG0780037453.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 26164599 26165199 26164599 ID=MsG0780037453.01;Name=MsG0780037453.01
Chr7 mRNA 26164599 26165199 26164599 ID=MsG0780037453.01.T01;Parent=MsG0780037453.01;Name=MsG0780037453.01.T01;_AED=0.37;_eAED=0.41;_QI=0|0|0|1|1|1|2|0|148
Chr7 exon 26164979 26165199 26164979 ID=MsG0780037453.01.T01:exon:3604;Parent=MsG0780037453.01.T01
Chr7 exon 26164599 26164824 26164599 ID=MsG0780037453.01.T01:exon:3603;Parent=MsG0780037453.01.T01
Chr7 CDS 26164979 26165199 26164979 ID=MsG0780037453.01.T01:cds;Parent=MsG0780037453.01.T01
Chr7 CDS 26164599 26164824 26164599 ID=MsG0780037453.01.T01:cds;Parent=MsG0780037453.01.T01
Gene Sequence

>MsG0780037453.01.T01

ATGGAACAATCGGTGGAAGATTCAGGAGAAAGGAAAATCCGTTTATCGAGGAGGCACGTCAAAGAGTTGTGTGGATCAGCTTCTGAGGATAGCAGGATTGAAGATGACGATCTTTTGATAATGTCTGATGTGGATGAGATTCCCAGTGCACACACAATCAATCTCTTGAGGTGGTGTGATGATATCCCTCCGATTCTTCATCTACAACTGAGGAATTACTTTGAATTTGTATTTAAGATGAAAGCGTACAGCCACTATGATCGAGTGCGATTCCCTCATTACTTAAACCCTGACCGGATTCAGAACGTGATATGCAAAGGTGCTGACTTATTCGACATGCTTCCTGAAGAGTATACATTTAAGGAGATTATCGGAAAACTAGGACCGATTCCTCATTCTTATTCAGCAGTGCATTTCCTCGGCATCTTCTGCATTTGTGTTAAATAA

Protein sequence

>MsG0780037453.01.T01

MEQSVEDSGERKIRLSRRHVKELCGSASEDSRIEDDDLLIMSDVDEIPSAHTINLLRWCDDIPPILHLQLRNYFEFVFKMKAYSHYDRVRFPHYLNPDRIQNVICKGADLFDMLPEEYTFKEIIGKLGPIPHSYSAVHFLGIFCICVK*