AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031505.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031505.01.T02 MTR_7g057770 100.000 98 0 0 1 98 20 117 6.21e-69 207
MsG0680031505.01.T02 MTR_1g023590 98.980 98 1 0 1 98 20 117 3.46e-68 205
MsG0680031505.01.T02 MTR_5g083790 95.918 98 4 0 1 98 20 117 1.18e-66 201
MsG0680031505.01.T01 MTR_7g057770 100.000 117 0 0 1 117 1 117 5.58e-84 246
MsG0680031505.01.T01 MTR_1g023590 99.145 117 1 0 1 117 1 117 3.43e-83 243
MsG0680031505.01.T01 MTR_5g083790 95.726 117 5 0 1 117 1 117 7.18e-81 238
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031505.01.T02 AT5G48760 92.857 98 7 0 1 98 20 117 2.99e-64 195
MsG0680031505.01.T02 AT5G48760 92.857 98 7 0 1 98 20 117 2.99e-64 195
MsG0680031505.01.T02 AT3G24830 90.816 98 9 0 1 98 20 117 8.27e-64 194
MsG0680031505.01.T02 AT4G13170 91.837 98 8 0 1 98 20 117 2.97e-63 192
MsG0680031505.01.T02 AT3G07110 87.755 98 12 0 1 98 20 117 4.71e-62 189
MsG0680031505.01.T02 AT3G07110 87.755 98 12 0 1 98 20 117 5.62e-62 189
MsG0680031505.01.T01 AT5G48760 92.308 117 9 0 1 117 1 117 1.89e-78 231
MsG0680031505.01.T01 AT5G48760 92.308 117 9 0 1 117 1 117 1.89e-78 231
MsG0680031505.01.T01 AT3G24830 90.598 117 11 0 1 117 1 117 6.65e-78 230
MsG0680031505.01.T01 AT4G13170 91.453 117 10 0 1 117 1 117 1.88e-77 229
MsG0680031505.01.T01 AT3G07110 88.889 117 13 0 1 117 1 117 1.02e-76 227
MsG0680031505.01.T01 AT3G07110 88.889 117 13 0 1 117 1 117 1.11e-76 227

Find 49 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGGTCCTATTCATTTC+AGG 0.115584 6:+21294213 MsG0680031505.01.T01:CDS
GGGCTCCTAGTAAGATCTTT+TGG 0.303037 6:+21294234 MsG0680031505.01.T01:CDS
GTGCTTCTGGTGGTCTTGTT+AGG 0.322378 6:+21294138 MsG0680031505.01.T01:CDS
TGAAGTATATGAGATTTCTT+AGG 0.358557 6:+21294168 MsG0680031505.01.T01:CDS
TGTGAAGAGATTTGTGCTTC+TGG 0.365693 6:+21294125 MsG0680031505.01.T01:CDS
TCATGGTCCTATTCATTTCA+GGG 0.370228 6:+21294214 MsG0680031505.01.T01:CDS
CCCGTATCGCACTACCTATC+CGG 0.389676 6:-21294406 None:intergenic
GATGCAAGGCACCACATGTT+AGG 0.404063 6:+21294041 MsG0680031505.01.T01:CDS
TGAAGTGGGAACACAGCATA+TGG 0.425151 6:-21294344 None:intergenic
CTAGGAGCCCTGAAATGAAT+AGG 0.426077 6:-21294221 None:intergenic
AATAGGACCATGAGAAGGTT+TGG 0.442500 6:-21294204 None:intergenic
GTGGAGAGGCGGCACTTGCC+AGG 0.444845 6:+21294294 MsG0680031505.01.T01:CDS
CCCGGATAGGTAGTGCGATA+CGG 0.461469 6:+21294405 MsG0680031505.01.T01:CDS
ACTACCTATCCGGGCTTCAT+AGG 0.465075 6:-21294396 None:intergenic
ATATGAGATTTCTTAGGAAG+AGG 0.471394 6:+21294174 MsG0680031505.01.T01:CDS
CTTCATAGGTTGTGAGGGAC+AGG 0.474578 6:-21294382 None:intergenic
CCGGATAGGTAGTGCGATAC+GGG 0.489064 6:+21294406 MsG0680031505.01.T01:CDS
GTACGCCAAAAGATCTTACT+AGG 0.507229 6:-21294239 None:intergenic
GGCGGCACTTGCCAGGATGA+AGG 0.510750 6:+21294301 MsG0680031505.01.T01:CDS
TCTCCACGCTTTGTCTTGTG+TGG 0.517762 6:-21294278 None:intergenic
CCCTCACAACCTATGAAGCC+CGG 0.531342 6:+21294387 MsG0680031505.01.T01:CDS
GTTGCAAAGGAGCTTCTCAA+TGG 0.552197 6:+21294080 MsG0680031505.01.T01:CDS
TCCGGGCTTCATAGGTTGTG+AGG 0.558923 6:-21294388 None:intergenic
ACTTGCTTCCATCGTTGCAA+AGG 0.560932 6:+21294067 MsG0680031505.01.T01:CDS
ACCCTCGTACACCTTCATCC+TGG 0.577964 6:-21294312 None:intergenic
ATACGGGTACGATACGATAC+GGG 0.579627 6:+21294422 MsG0680031505.01.T01:CDS
CAAGACAAAGCGTGGAGAGG+CGG 0.579684 6:+21294283 MsG0680031505.01.T01:CDS
GGGAACACAGCATATGGAGG+TGG 0.583655 6:-21294338 None:intergenic
GAGAAGCTCCTTTGCAACGA+TGG 0.584142 6:-21294075 None:intergenic
GAAGAGATTTGTGCTTCTGG+TGG 0.586152 6:+21294128 MsG0680031505.01.T01:CDS
GATACGGGTACGATACGATA+CGG 0.587549 6:+21294421 MsG0680031505.01.T01:CDS
CCGTATCGCACTACCTATCC+GGG 0.598753 6:-21294405 None:intergenic
CCGGGCTTCATAGGTTGTGA+GGG 0.598941 6:-21294387 None:intergenic
GGAGCTTCTCAATGGTCAGA+AGG 0.600116 6:+21294088 MsG0680031505.01.T01:CDS
AGGTATCTGCGCAAAGAGGG+TGG 0.610561 6:+21294013 MsG0680031505.01.T01:CDS
TTCAGGTATCTGCGCAAAGA+GGG 0.610853 6:+21294010 MsG0680031505.01.T01:CDS
ACTTGAGAACGAGAATGAAG+TGG 0.614520 6:-21294359 None:intergenic
TGCCAGGATGAAGGTGTACG+AGG 0.620108 6:+21294310 MsG0680031505.01.T01:CDS
ATGAACACCAAACCTTCTCA+TGG 0.620990 6:+21294197 MsG0680031505.01.T01:CDS
CTTGAGAACGAGAATGAAGT+GGG 0.625690 6:-21294358 None:intergenic
ACAACCTATGAAGCCCGGAT+AGG 0.628389 6:+21294392 MsG0680031505.01.T01:CDS
GTTCAGGTATCTGCGCAAAG+AGG 0.631106 6:+21294009 MsG0680031505.01.T01:CDS
ATTCCACACAAGACAAAGCG+TGG 0.632644 6:+21294275 MsG0680031505.01.T01:CDS
AGAGGGTGGTGATTGATGCA+AGG 0.637601 6:+21294027 MsG0680031505.01.T01:CDS
ACACAAGACAAAGCGTGGAG+AGG 0.661685 6:+21294280 MsG0680031505.01.T01:CDS
AAATGAATAGGACCATGAGA+AGG 0.667076 6:-21294209 None:intergenic
GCCAGGATGAAGGTGTACGA+GGG 0.670691 6:+21294311 MsG0680031505.01.T01:CDS
AGTGGGAACACAGCATATGG+AGG 0.689265 6:-21294341 None:intergenic
AAGCAAGTCTACCTAACATG+TGG 0.692845 6:-21294052 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGAAGTATATGAGATTTCTT+AGG + Chr6:21294168-21294187 MsG0680031505.01.T01:CDS 25.0%
ATATGAGATTTCTTAGGAAG+AGG + Chr6:21294174-21294193 MsG0680031505.01.T01:CDS 30.0%
AAATGAATAGGACCATGAGA+AGG - Chr6:21294212-21294231 None:intergenic 35.0%
TCATGGTCCTATTCATTTCA+GGG + Chr6:21294214-21294233 MsG0680031505.01.T01:CDS 35.0%
AAGCAAGTCTACCTAACATG+TGG - Chr6:21294055-21294074 None:intergenic 40.0%
AATAGGACCATGAGAAGGTT+TGG - Chr6:21294207-21294226 None:intergenic 40.0%
ACTTGAGAACGAGAATGAAG+TGG - Chr6:21294362-21294381 None:intergenic 40.0%
ATGAACACCAAACCTTCTCA+TGG + Chr6:21294197-21294216 MsG0680031505.01.T01:CDS 40.0%
CTCATGGTCCTATTCATTTC+AGG + Chr6:21294213-21294232 MsG0680031505.01.T01:CDS 40.0%
CTTGAGAACGAGAATGAAGT+GGG - Chr6:21294361-21294380 None:intergenic 40.0%
GTACGCCAAAAGATCTTACT+AGG - Chr6:21294242-21294261 None:intergenic 40.0%
TGTGAAGAGATTTGTGCTTC+TGG + Chr6:21294125-21294144 MsG0680031505.01.T01:CDS 40.0%
ACTTGCTTCCATCGTTGCAA+AGG + Chr6:21294067-21294086 MsG0680031505.01.T01:CDS 45.0%
ATACGGGTACGATACGATAC+GGG + Chr6:21294422-21294441 MsG0680031505.01.T01:CDS 45.0%
ATTCCACACAAGACAAAGCG+TGG + Chr6:21294275-21294294 MsG0680031505.01.T01:CDS 45.0%
CTAGGAGCCCTGAAATGAAT+AGG - Chr6:21294224-21294243 None:intergenic 45.0%
GAAGAGATTTGTGCTTCTGG+TGG + Chr6:21294128-21294147 MsG0680031505.01.T01:CDS 45.0%
GATACGGGTACGATACGATA+CGG + Chr6:21294421-21294440 MsG0680031505.01.T01:CDS 45.0%
GTTGCAAAGGAGCTTCTCAA+TGG + Chr6:21294080-21294099 MsG0680031505.01.T01:CDS 45.0%
TGAAGTGGGAACACAGCATA+TGG - Chr6:21294347-21294366 None:intergenic 45.0%
TTCAGGTATCTGCGCAAAGA+GGG + Chr6:21294010-21294029 MsG0680031505.01.T01:CDS 45.0%
! ATCTTTTGGCGTACTGTTCG+TGG + Chr6:21294248-21294267 MsG0680031505.01.T01:CDS 45.0%
! GGGCTCCTAGTAAGATCTTT+TGG + Chr6:21294234-21294253 MsG0680031505.01.T01:CDS 45.0%
ACAACCTATGAAGCCCGGAT+AGG + Chr6:21294392-21294411 MsG0680031505.01.T01:CDS 50.0%
ACACAAGACAAAGCGTGGAG+AGG + Chr6:21294280-21294299 MsG0680031505.01.T01:CDS 50.0%
ACTACCTATCCGGGCTTCAT+AGG - Chr6:21294399-21294418 None:intergenic 50.0%
AGAGGGTGGTGATTGATGCA+AGG + Chr6:21294027-21294046 MsG0680031505.01.T01:CDS 50.0%
AGTGGGAACACAGCATATGG+AGG - Chr6:21294344-21294363 None:intergenic 50.0%
CTTCATAGGTTGTGAGGGAC+AGG - Chr6:21294385-21294404 None:intergenic 50.0%
GAGAAGCTCCTTTGCAACGA+TGG - Chr6:21294078-21294097 None:intergenic 50.0%
GGAGCTTCTCAATGGTCAGA+AGG + Chr6:21294088-21294107 MsG0680031505.01.T01:CDS 50.0%
GTTCAGGTATCTGCGCAAAG+AGG + Chr6:21294009-21294028 MsG0680031505.01.T01:CDS 50.0%
TCTCCACGCTTTGTCTTGTG+TGG - Chr6:21294281-21294300 None:intergenic 50.0%
! GATGCAAGGCACCACATGTT+AGG + Chr6:21294041-21294060 MsG0680031505.01.T01:CDS 50.0%
!! GTGCTTCTGGTGGTCTTGTT+AGG + Chr6:21294138-21294157 MsG0680031505.01.T01:CDS 50.0%
ACCCTCGTACACCTTCATCC+TGG - Chr6:21294315-21294334 None:intergenic 55.0%
AGGTATCTGCGCAAAGAGGG+TGG + Chr6:21294013-21294032 MsG0680031505.01.T01:CDS 55.0%
CAAGACAAAGCGTGGAGAGG+CGG + Chr6:21294283-21294302 MsG0680031505.01.T01:CDS 55.0%
CCCGGATAGGTAGTGCGATA+CGG + Chr6:21294405-21294424 MsG0680031505.01.T01:CDS 55.0%
CCCGTATCGCACTACCTATC+CGG - Chr6:21294409-21294428 None:intergenic 55.0%
CCCTCACAACCTATGAAGCC+CGG + Chr6:21294387-21294406 MsG0680031505.01.T01:CDS 55.0%
CCGGATAGGTAGTGCGATAC+GGG + Chr6:21294406-21294425 MsG0680031505.01.T01:CDS 55.0%
CCGGGCTTCATAGGTTGTGA+GGG - Chr6:21294390-21294409 None:intergenic 55.0%
CCGTATCGCACTACCTATCC+GGG - Chr6:21294408-21294427 None:intergenic 55.0%
GGGAACACAGCATATGGAGG+TGG - Chr6:21294341-21294360 None:intergenic 55.0%
TCCGGGCTTCATAGGTTGTG+AGG - Chr6:21294391-21294410 None:intergenic 55.0%
! GCCAGGATGAAGGTGTACGA+GGG + Chr6:21294311-21294330 MsG0680031505.01.T01:CDS 55.0%
! TGCCAGGATGAAGGTGTACG+AGG + Chr6:21294310-21294329 MsG0680031505.01.T01:CDS 55.0%
GGCGGCACTTGCCAGGATGA+AGG + Chr6:21294301-21294320 MsG0680031505.01.T01:CDS 65.0%
GTGGAGAGGCGGCACTTGCC+AGG + Chr6:21294294-21294313 MsG0680031505.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 21293999 21294475 21293999 ID=MsG0680031505.01;Name=MsG0680031505.01
Chr6 mRNA 21293999 21294475 21293999 ID=MsG0680031505.01.T01;Parent=MsG0680031505.01;Name=MsG0680031505.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|158
Chr6 exon 21293999 21294475 21293999 ID=MsG0680031505.01.T01:exon:13525;Parent=MsG0680031505.01.T01
Chr6 CDS 21293999 21294475 21293999 ID=MsG0680031505.01.T01:cds;Parent=MsG0680031505.01.T01
Chr6 mRNA 21294056 21294475 21294056 ID=MsG0680031505.01.T02;Parent=MsG0680031505.01;Name=MsG0680031505.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|139
Chr6 exon 21294056 21294475 21294056 ID=MsG0680031505.01.T02:exon:13526;Parent=MsG0680031505.01.T02
Chr6 CDS 21294056 21294475 21294056 ID=MsG0680031505.01.T02:cds;Parent=MsG0680031505.01.T02
Gene Sequence

>MsG0680031505.01.T02

ATGTTAGGTAGACTTGCTTCCATCGTTGCAAAGGAGCTTCTCAATGGTCAGAAGGTTGTTCTTGTGAGATGTGAAGAGATTTGTGCTTCTGGTGGTCTTGTTAGGCAGAAGATGAAGTATATGAGATTTCTTAGGAAGAGGATGAACACCAAACCTTCTCATGGTCCTATTCATTTCAGGGCTCCTAGTAAGATCTTTTGGCGTACTGTTCGTGGAATGATTCCACACAAGACAAAGCGTGGAGAGGCGGCACTTGCCAGGATGAAGGTGTACGAGGGTATTCCACCTCCATATGCTGTGTTCCCACTTCATTCTCGTTCTCAAGTCCTGTCCCTCACAACCTATGAAGCCCGGATAGGTAGTGCGATACGGGTACGATACGATACGGGTACGCGAATACGCGAAATTTTTAAAATTTAG

>MsG0680031505.01.T01

ATGGTGTCTGGTTCAGGTATCTGCGCAAAGAGGGTGGTGATTGATGCAAGGCACCACATGTTAGGTAGACTTGCTTCCATCGTTGCAAAGGAGCTTCTCAATGGTCAGAAGGTTGTTCTTGTGAGATGTGAAGAGATTTGTGCTTCTGGTGGTCTTGTTAGGCAGAAGATGAAGTATATGAGATTTCTTAGGAAGAGGATGAACACCAAACCTTCTCATGGTCCTATTCATTTCAGGGCTCCTAGTAAGATCTTTTGGCGTACTGTTCGTGGAATGATTCCACACAAGACAAAGCGTGGAGAGGCGGCACTTGCCAGGATGAAGGTGTACGAGGGTATTCCACCTCCATATGCTGTGTTCCCACTTCATTCTCGTTCTCAAGTCCTGTCCCTCACAACCTATGAAGCCCGGATAGGTAGTGCGATACGGGTACGATACGATACGGGTACGCGAATACGCGAAATTTTTAAAATTTAG

Protein sequence

>MsG0680031505.01.T02

MLGRLASIVAKELLNGQKVVLVRCEEICASGGLVRQKMKYMRFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGEAALARMKVYEGIPPPYAVFPLHSRSQVLSLTTYEARIGSAIRVRYDTGTRIREIFKI*

>MsG0680031505.01.T01

MVSGSGICAKRVVIDARHHMLGRLASIVAKELLNGQKVVLVRCEEICASGGLVRQKMKYMRFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGEAALARMKVYEGIPPPYAVFPLHSRSQVLSLTTYEARIGSAIRVRYDTGTRIREIFKI*