AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026771.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026771.01.T01 MTR_4g105330 96.739 184 6 0 1 184 119 302 2.36e-131 370
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026771.01.T01 AT3G48210 42.473 186 98 4 2 184 133 312 8.65e-38 132

Find 37 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCCATCTCTTGGTATAAC+AGG 0.291680 5:-44336843 MsG0580026771.01.T01:CDS
CATCTGTCTTGTTTAATTCA+TGG 0.302544 5:+44336484 None:intergenic
GTAAAAGAGTACTCCTCATT+TGG 0.343740 5:+44336665 None:intergenic
GAAGCAGAAACAGGAGATAA+AGG 0.378138 5:+44335791 None:intergenic
TCGCCAGTCTCCACGTTTGA+AGG 0.380013 5:-44335762 MsG0580026771.01.T01:CDS
TCTTGGTATAACAGGGTACT+TGG 0.397967 5:-44336835 MsG0580026771.01.T01:CDS
CGAACAGATGAAGCAGAAAC+AGG 0.430200 5:+44335782 None:intergenic
AGTTCGCAGAAGGAAAAGTT+TGG 0.432380 5:-44335816 MsG0580026771.01.T01:CDS
TCATCCGCTCCTGGCACATC+TGG 0.434205 5:-44335917 MsG0580026771.01.T01:CDS
AGGAGCGGATGAAGATATAA+AGG 0.452608 5:+44335928 None:intergenic
TTTATATCTTCATCCGCTCC+TGG 0.466324 5:-44335926 MsG0580026771.01.T01:CDS
CGTTGTGAACCTTCCTTCGA+TGG 0.489675 5:-44336522 MsG0580026771.01.T01:CDS
CTTCGATGGTATTGAAGAGC+TGG 0.509662 5:-44336508 MsG0580026771.01.T01:CDS
ATACCTTCAAACGTGGAGAC+TGG 0.510056 5:+44335759 None:intergenic
TTAAGTTTGTCAGAGTAATG+AGG 0.518116 5:-44336455 MsG0580026771.01.T01:CDS
GAGCATGAAGTGGAACGCTC+TGG 0.532714 5:-44335866 MsG0580026771.01.T01:CDS
ATCTGAAGGGAGGTTGAATG+AGG 0.533325 5:-44336893 MsG0580026771.01.T01:CDS
GATATAAAGGCAGATTCTCC+AGG 0.534729 5:+44335941 None:intergenic
GAAGCTTTGGCAGCATCTGA+AGG 0.537582 5:-44336907 None:intergenic
AAACACAGGAGGCCATCTCT+TGG 0.552693 5:-44336852 MsG0580026771.01.T01:CDS
TAGAAGTGATTCTACAGCCA+TGG 0.573031 5:-44335894 MsG0580026771.01.T01:CDS
AAGCTTTGGCAGCATCTGAA+GGG 0.586020 5:-44336906 None:intergenic
TCACTTCTACCAGATGTGCC+AGG 0.586982 5:+44335908 None:intergenic
TTTCAAGAAGCATTGGCACA+AGG 0.601367 5:-44336426 MsG0580026771.01.T01:intron
TACCCTGTTATACCAAGAGA+TGG 0.606063 5:+44336840 None:intergenic
CAGCTCTTCAATACCATCGA+AGG 0.622116 5:+44336509 None:intergenic
CTTTGGCAGCATCTGAAGGG+AGG 0.622951 5:-44336903 MsG0580026771.01.T01:CDS
AAATTTGAAGAATCCAAATG+AGG 0.623545 5:-44336678 MsG0580026771.01.T01:CDS
CATGAATTAAACAAGACAGA+TGG 0.623903 5:-44336483 MsG0580026771.01.T01:CDS
TCTTCAATACCATCGAAGGA+AGG 0.637490 5:+44336513 None:intergenic
CATGGAAAATGAGCATGAAG+TGG 0.637509 5:-44335876 MsG0580026771.01.T01:CDS
GGCCATCTCTTGGTATAACA+GGG 0.647513 5:-44336842 MsG0580026771.01.T01:CDS
TCTGAAGGGAGGTTGAATGA+GGG 0.651760 5:-44336892 MsG0580026771.01.T01:CDS
TCTACCAGATGTGCCAGGAG+CGG 0.654573 5:+44335913 None:intergenic
TGAACATACAGATGAAACAC+AGG 0.659863 5:-44336866 MsG0580026771.01.T01:CDS
GTAATTCATACCTTCAAACG+TGG 0.717846 5:+44335752 None:intergenic
ACATACAGATGAAACACAGG+AGG 0.726406 5:-44336863 MsG0580026771.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTATAAGTATTTTTATCA+TGG + Chr5:44336025-44336044 None:intergenic 15.0%
!!! TCATTATAATATGAAGTATT+TGG + Chr5:44336419-44336438 None:intergenic 15.0%
!!! TTATCATTTTATAATTCAAG+CGG + Chr5:44336335-44336354 None:intergenic 15.0%
!! CATAAGCAAAAAAAAGTTAA+TGG + Chr5:44335880-44335899 None:intergenic 20.0%
!! GTATATAAAATCATAAATGC+AGG + Chr5:44336056-44336075 None:intergenic 20.0%
!! TTAATAGAATCACTTCATAT+TGG + Chr5:44336379-44336398 None:intergenic 20.0%
!!! CATTAACTTTTTTTTGCTTA+TGG - Chr5:44335878-44335897 MsG0580026771.01.T01:CDS 20.0%
! AAATTTGAAGAATCCAAATG+AGG - Chr5:44335980-44335999 MsG0580026771.01.T01:intron 25.0%
! ATTATTGAACTGTTCAAACA+TGG - Chr5:44336496-44336515 MsG0580026771.01.T01:CDS 25.0%
! GATTTAATCGCTATTAAACA+GGG - Chr5:44336670-44336689 MsG0580026771.01.T01:CDS 25.0%
! GTTCAATAATACATATGAAC+TGG + Chr5:44336486-44336505 None:intergenic 25.0%
! TGATTTAATCGCTATTAAAC+AGG - Chr5:44336669-44336688 MsG0580026771.01.T01:CDS 25.0%
! TTGTTGGAAATTGAATGAAA+GGG - Chr5:44336625-44336644 MsG0580026771.01.T01:intron 25.0%
!! GAAAAAATTTCAAGAAGCAT+TGG - Chr5:44336225-44336244 MsG0580026771.01.T01:intron 25.0%
AAAGATAAAAGAGATGGTGT+AGG + Chr5:44336108-44336127 None:intergenic 30.0%
ACACTGAAAGATAAAAGAGA+TGG + Chr5:44336114-44336133 None:intergenic 30.0%
CATGAATTAAACAAGACAGA+TGG - Chr5:44336175-44336194 MsG0580026771.01.T01:intron 30.0%
CTTATTAGTTATGCTCTTCA+GGG - Chr5:44335934-44335953 MsG0580026771.01.T01:CDS 30.0%
CTTGTTGGAAATTGAATGAA+AGG - Chr5:44336624-44336643 MsG0580026771.01.T01:intron 30.0%
TAAATAGTATCTTCGCTTGT+TGG - Chr5:44336609-44336628 MsG0580026771.01.T01:intron 30.0%
TTAAGTTTGTCAGAGTAATG+AGG - Chr5:44336203-44336222 MsG0580026771.01.T01:intron 30.0%
TTATTAGTTATGCTCTTCAG+GGG - Chr5:44335935-44335954 MsG0580026771.01.T01:CDS 30.0%
!! CATCTGTCTTGTTTAATTCA+TGG + Chr5:44336177-44336196 None:intergenic 30.0%
!! GTTTTAACAACAGTTAATCC+TGG - Chr5:44336699-44336718 MsG0580026771.01.T01:CDS 30.0%
!! TACTTGGTTTTCATGTCAAA+GGG - Chr5:44335839-44335858 MsG0580026771.01.T01:CDS 30.0%
AGAAAAAACAAGTTCGCAGA+AGG - Chr5:44336832-44336851 MsG0580026771.01.T01:CDS 35.0%
ATATTGGAGCTCTGCATATT+TGG + Chr5:44336363-44336382 None:intergenic 35.0%
CCTTATTAGTTATGCTCTTC+AGG - Chr5:44335933-44335952 MsG0580026771.01.T01:CDS 35.0%
TGAACATACAGATGAAACAC+AGG - Chr5:44335792-44335811 MsG0580026771.01.T01:CDS 35.0%
! CCTGAAGAGCATAACTAATA+AGG + Chr5:44335936-44335955 None:intergenic 35.0%
!! ACTTGGTTTTCATGTCAAAG+GGG - Chr5:44335840-44335859 MsG0580026771.01.T01:CDS 35.0%
!! GTAAAAGAGTACTCCTCATT+TGG + Chr5:44335996-44336015 None:intergenic 35.0%
!! GTACTTGGTTTTCATGTCAA+AGG - Chr5:44335838-44335857 MsG0580026771.01.T01:CDS 35.0%
ACATACAGATGAAACACAGG+AGG - Chr5:44335795-44335814 MsG0580026771.01.T01:CDS 40.0%
AGGAGCGGATGAAGATATAA+AGG + Chr5:44336733-44336752 None:intergenic 40.0%
AGTTCGCAGAAGGAAAAGTT+TGG - Chr5:44336842-44336861 MsG0580026771.01.T01:CDS 40.0%
ATGAGTGTATGCATGTGATG+TGG - Chr5:44336281-44336300 MsG0580026771.01.T01:intron 40.0%
CATGGAAAATGAGCATGAAG+TGG - Chr5:44336782-44336801 MsG0580026771.01.T01:intron 40.0%
GAAGCAGAAACAGGAGATAA+AGG + Chr5:44336870-44336889 None:intergenic 40.0%
GATATAAAGGCAGATTCTCC+AGG + Chr5:44336720-44336739 None:intergenic 40.0%
TACCCTGTTATACCAAGAGA+TGG + Chr5:44335821-44335840 None:intergenic 40.0%
TAGAAGTGATTCTACAGCCA+TGG - Chr5:44336764-44336783 MsG0580026771.01.T01:intron 40.0%
TCTTCAATACCATCGAAGGA+AGG + Chr5:44336148-44336167 None:intergenic 40.0%
TGAGTGTATGCATGTGATGT+GGG - Chr5:44336282-44336301 MsG0580026771.01.T01:intron 40.0%
TTTATATCTTCATCCGCTCC+TGG - Chr5:44336732-44336751 MsG0580026771.01.T01:intron 40.0%
! CACTTCATGCTCATTTTCCA+TGG + Chr5:44336784-44336803 None:intergenic 40.0%
! TCTTGGTATAACAGGGTACT+TGG - Chr5:44335823-44335842 MsG0580026771.01.T01:CDS 40.0%
!! CTTGGTTTTCATGTCAAAGG+GGG - Chr5:44335841-44335860 MsG0580026771.01.T01:CDS 40.0%
!! TTTCAAGAAGCATTGGCACA+AGG - Chr5:44336232-44336251 MsG0580026771.01.T01:intron 40.0%
AGGCCATCTCTTGGTATAAC+AGG - Chr5:44335815-44335834 MsG0580026771.01.T01:CDS 45.0%
ATACCTTCAAACGTGGAGAC+TGG + Chr5:44336902-44336921 None:intergenic 45.0%
ATCTGAAGGGAGGTTGAATG+AGG - Chr5:44335765-44335784 MsG0580026771.01.T01:CDS 45.0%
CAGCTCTTCAATACCATCGA+AGG + Chr5:44336152-44336171 None:intergenic 45.0%
CGAACAGATGAAGCAGAAAC+AGG + Chr5:44336879-44336898 None:intergenic 45.0%
GGCCATCTCTTGGTATAACA+GGG - Chr5:44335816-44335835 MsG0580026771.01.T01:CDS 45.0%
TCTGAAGGGAGGTTGAATGA+GGG - Chr5:44335766-44335785 MsG0580026771.01.T01:CDS 45.0%
! CTTCGATGGTATTGAAGAGC+TGG - Chr5:44336150-44336169 MsG0580026771.01.T01:intron 45.0%
AAACACAGGAGGCCATCTCT+TGG - Chr5:44335806-44335825 MsG0580026771.01.T01:CDS 50.0%
CGTTGTGAACCTTCCTTCGA+TGG - Chr5:44336136-44336155 MsG0580026771.01.T01:intron 50.0%
GATGTGGGAGCTCTGCATAT+TGG - Chr5:44336297-44336316 MsG0580026771.01.T01:intron 50.0%
TCACTTCTACCAGATGTGCC+AGG + Chr5:44336753-44336772 None:intergenic 50.0%
TTTCATGTCAAAGGGGGACG+TGG - Chr5:44335847-44335866 MsG0580026771.01.T01:CDS 50.0%
GAGCATGAAGTGGAACGCTC+TGG - Chr5:44336792-44336811 MsG0580026771.01.T01:intron 55.0%
TCGCCAGTCTCCACGTTTGA+AGG - Chr5:44336896-44336915 MsG0580026771.01.T01:CDS 55.0%
TCTACCAGATGTGCCAGGAG+CGG + Chr5:44336748-44336767 None:intergenic 55.0%
TCATCCGCTCCTGGCACATC+TGG - Chr5:44336741-44336760 MsG0580026771.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 44335757 44336923 44335757 ID=MsG0580026771.01;Name=MsG0580026771.01
Chr5 mRNA 44335757 44336923 44335757 ID=MsG0580026771.01.T01;Parent=MsG0580026771.01;Name=MsG0580026771.01.T01;_AED=0.50;_eAED=0.50;_QI=0|1|0.5|1|1|1|4|0|185
Chr5 exon 44336812 44336923 44336812 ID=MsG0580026771.01.T01:exon:18313;Parent=MsG0580026771.01.T01
Chr5 exon 44336636 44336725 44336636 ID=MsG0580026771.01.T01:exon:18312;Parent=MsG0580026771.01.T01
Chr5 exon 44336427 44336549 44336427 ID=MsG0580026771.01.T01:exon:18311;Parent=MsG0580026771.01.T01
Chr5 exon 44335757 44335989 44335757 ID=MsG0580026771.01.T01:exon:18310;Parent=MsG0580026771.01.T01
Chr5 CDS 44336812 44336923 44336812 ID=MsG0580026771.01.T01:cds;Parent=MsG0580026771.01.T01
Chr5 CDS 44336636 44336725 44336636 ID=MsG0580026771.01.T01:cds;Parent=MsG0580026771.01.T01
Chr5 CDS 44336427 44336549 44336427 ID=MsG0580026771.01.T01:cds;Parent=MsG0580026771.01.T01
Chr5 CDS 44335757 44335989 44335757 ID=MsG0580026771.01.T01:cds;Parent=MsG0580026771.01.T01
Gene Sequence

>MsG0580026771.01.T01

TTGGCAGCATCTGAAGGGAGGTTGAATGAGGGCATTGAACATACAGATGAAACACAGGAGGCCATCTCTTGGTATAACAGGGTACTTGGTTTTCATGTCAAAGGGGGACGTGGGGTAAAATTCACGTTCAAGAATATAAATTTGAAGAATCCAAATGAGGAGTACTCTTTTACTGTCTTCCATGATAAAAATACTTATACATTGTTACGTTGTGAACCTTCCTTCGATGGTATTGAAGAGCTGGTCCATGAATTAAACAAGACAGATGGTCTGTTTAAGTTTGTCAGAGTAATGAGGAAAAAATTTCAAGAAGCATTGGCACAAGGGAGCTTAGTTTTAACAACAGTTAATCCTGGAGAATCTGCCTTTATATCTTCATCCGCTCCTGGCACATCTGGTAGAAGTGATTCTACAGCCATGGAAAATGAGCATGAAGTGGAACGCTCTGGCAGTAGTGCACAATTAAAGAAAAAACAAGTTCGCAGAAGGAAAAGTTTGGCACCTTTATCTCCTGTTTCTGCTTCATCTGTTCGCCAGTCTCCACGTTTGAAGGTATGA

Protein sequence

>MsG0580026771.01.T01

LAASEGRLNEGIEHTDETQEAISWYNRVLGFHVKGGRGVKFTFKNINLKNPNEEYSFTVFHDKNTYTLLRCEPSFDGIEELVHELNKTDGLFKFVRVMRKKFQEALAQGSLVLTTVNPGESAFISSSAPGTSGRSDSTAMENEHEVERSGSSAQLKKKQVRRRKSLAPLSPVSASSVRQSPRLKV*