AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012528.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012528.01.T01 MTR_1g087510 67.797 59 19 0 25 83 114 172 1.79e-25 93.2
MsG0380012528.01.T01 MTR_6g465600 45.783 83 45 0 1 83 228 310 8.24e-25 94.4
MsG0380012528.01.T01 MTR_7g050355 53.030 66 31 0 10 75 160 225 5.03e-18 77.0
MsG0380012528.01.T01 MTR_1g052720 42.466 73 42 0 11 83 233 305 1.07e-17 75.1
MsG0380012528.01.T01 MTR_8g064960 61.364 44 17 0 1 44 56 99 8.35e-16 66.6
MsG0380012528.01.T01 MTR_0246s0040 43.333 60 33 1 3 62 230 288 5.18e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCCAGGAGTATTTC+TGG 0.174413 3:-19267425 MsG0380012528.01.T01:CDS
GTATACATGGAAAATGCTTT+TGG 0.290966 3:-19267658 None:intergenic
TGCTCCTTTGGTCAGCTTAC+GGG 0.310186 3:+19267572 None:intergenic
GAACTTTATAGTTGCTCCTT+TGG 0.350273 3:+19267560 None:intergenic
TTGCTCCTTTGGTCAGCTTA+CGG 0.366158 3:+19267571 None:intergenic
ACTATAAAGTTCGGTGTTAC+CGG 0.377234 3:-19267549 MsG0380012528.01.T01:CDS
CGGATCAAGTTATCAATTCC+AGG 0.384857 3:-19267436 MsG0380012528.01.T01:CDS
GCCTGCGTCTGGAAACGTTT+GGG 0.414200 3:-19267463 MsG0380012528.01.T01:CDS
AGAACAAGGATGCCTGCGTC+TGG 0.423335 3:-19267474 MsG0380012528.01.T01:CDS
TGCCTGCGTCTGGAAACGTT+TGG 0.477125 3:-19267464 MsG0380012528.01.T01:CDS
CATCGCAAAGTGCTTGCCAC+TGG 0.500453 3:+19267595 None:intergenic
GCAAAGTGCTTGCCACTGGC+CGG 0.521123 3:+19267599 None:intergenic
AAAGGAGCAACTATAAAGTT+CGG 0.521811 3:-19267558 MsG0380012528.01.T01:CDS
GTTCTCAGAATCAACATTGG+AGG 0.530223 3:+19267492 None:intergenic
CAATGTTGATTCTGAGAACA+AGG 0.535839 3:-19267488 MsG0380012528.01.T01:CDS
CTCAGAATCAACATTGGAGG+TGG 0.555144 3:+19267495 None:intergenic
CTTGTTCTCAGAATCAACAT+TGG 0.555316 3:+19267489 None:intergenic
TCCCAAACGTTTCCAGACGC+AGG 0.563324 3:+19267462 None:intergenic
TCTGGAAACGTTTGGGAAGG+CGG 0.564815 3:-19267456 MsG0380012528.01.T01:CDS
GCGTCTGGAAACGTTTGGGA+AGG 0.592286 3:-19267459 MsG0380012528.01.T01:CDS
GATGCCCGTAAGCTGACCAA+AGG 0.662152 3:-19267576 MsG0380012528.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGGAGCAACTATAAAGTT+CGG - Chr3:19267503-19267522 MsG0380012528.01.T01:CDS 30.0%
! AATTTTTCCAGAAATACTCC+TGG + Chr3:19267646-19267665 None:intergenic 30.0%
! TTGGATTTTTGTCATGATGA+TGG - Chr3:19267422-19267441 MsG0380012528.01.T01:CDS 30.0%
CTTGTTCTCAGAATCAACAT+TGG + Chr3:19267575-19267594 None:intergenic 35.0%
GAACTTTATAGTTGCTCCTT+TGG + Chr3:19267504-19267523 None:intergenic 35.0%
!! ACTATAAAGTTCGGTGTTAC+CGG - Chr3:19267512-19267531 MsG0380012528.01.T01:CDS 35.0%
!! ATCAATTCCAGGAGTATTTC+TGG - Chr3:19267636-19267655 MsG0380012528.01.T01:CDS 35.0%
!! CAATGTTGATTCTGAGAACA+AGG - Chr3:19267573-19267592 MsG0380012528.01.T01:CDS 35.0%
CGGATCAAGTTATCAATTCC+AGG - Chr3:19267625-19267644 MsG0380012528.01.T01:CDS 40.0%
GTTCTCAGAATCAACATTGG+AGG + Chr3:19267572-19267591 None:intergenic 40.0%
!!! AACCTTGTTTTTAGCTGCTC+CGG + Chr3:19267534-19267553 None:intergenic 40.0%
CTCAGAATCAACATTGGAGG+TGG + Chr3:19267569-19267588 None:intergenic 45.0%
TACCGGAGCAGCTAAAAACA+AGG - Chr3:19267529-19267548 MsG0380012528.01.T01:CDS 45.0%
TTGCTCCTTTGGTCAGCTTA+CGG + Chr3:19267493-19267512 None:intergenic 45.0%
!!! GGTGTGTCATGCACTTTTTC+CGG - Chr3:19267443-19267462 MsG0380012528.01.T01:CDS 45.0%
TCTGGAAACGTTTGGGAAGG+CGG - Chr3:19267605-19267624 MsG0380012528.01.T01:CDS 50.0%
TGCTCCTTTGGTCAGCTTAC+GGG + Chr3:19267492-19267511 None:intergenic 50.0%
AGAACAAGGATGCCTGCGTC+TGG - Chr3:19267587-19267606 MsG0380012528.01.T01:CDS 55.0%
GATGCCCGTAAGCTGACCAA+AGG - Chr3:19267485-19267504 MsG0380012528.01.T01:CDS 55.0%
GCCTGCGTCTGGAAACGTTT+GGG - Chr3:19267598-19267617 MsG0380012528.01.T01:CDS 55.0%
GCGTCTGGAAACGTTTGGGA+AGG - Chr3:19267602-19267621 MsG0380012528.01.T01:CDS 55.0%
TCCCAAACGTTTCCAGACGC+AGG + Chr3:19267602-19267621 None:intergenic 55.0%
TGCCTGCGTCTGGAAACGTT+TGG - Chr3:19267597-19267616 MsG0380012528.01.T01:CDS 55.0%
! CATCGCAAAGTGCTTGCCAC+TGG + Chr3:19267469-19267488 None:intergenic 55.0%
!!! CATGCACTTTTTCCGGCCAG+TGG - Chr3:19267450-19267469 MsG0380012528.01.T01:CDS 55.0%
! GCAAAGTGCTTGCCACTGGC+CGG + Chr3:19267465-19267484 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 19267416 19267667 19267416 ID=MsG0380012528.01;Name=MsG0380012528.01
Chr3 mRNA 19267416 19267667 19267416 ID=MsG0380012528.01.T01;Parent=MsG0380012528.01;Name=MsG0380012528.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|83
Chr3 exon 19267416 19267667 19267416 ID=MsG0380012528.01.T01:exon:18418;Parent=MsG0380012528.01.T01
Chr3 CDS 19267416 19267667 19267416 ID=MsG0380012528.01.T01:cds;Parent=MsG0380012528.01.T01
Gene Sequence

>MsG0380012528.01.T01

ATGCTTTTGGATTTTTGTCATGATGATGGTGTGTCATGCACTTTTTCCGGCCAGTGGCAAGCACTTTGCGATGCCCGTAAGCTGACCAAAGGAGCAACTATAAAGTTCGGTGTTACCGGAGCAGCTAAAAACAAGGTTGTCTATCTTTGTCCACCTCCAATGTTGATTCTGAGAACAAGGATGCCTGCGTCTGGAAACGTTTGGGAAGGCGGATCAAGTTATCAATTCCAGGAGTATTTCTGGAAAAATTAG

Protein sequence

>MsG0380012528.01.T01

MLLDFCHDDGVSCTFSGQWQALCDARKLTKGATIKFGVTGAAKNKVVYLCPPPMLILRTRMPASGNVWEGGSSYQFQEYFWKN*