AlfalfaGEDB | MsG0580027169.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027169.01

KEGG: K11550
GO: -
PFAM: PF08234

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027169.01.T01	XP_039689723.1	94.595	222	12	0	1	222	78	299	9.44E-152	434

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027169.01.T01	Q93VK9	37.991	229	126	6	1	222	93	312	4.27E-39	140

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027169.01.T01	A0A072UQ27	93.333	225	12	1	1	222	78	302	5.03e-150	428

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0580027169.01	0.801951	7.066143e-49	3.418578e-46
MsG0080048764.01	MsG0580027169.01	0.802203	6.272039e-49	3.053905e-46
MsG0180000065.01	MsG0580027169.01	0.810224	1.282166e-50	7.684777e-48
MsG0180001006.01	MsG0580027169.01	0.844815	5.985902e-59	9.779228e-56
MsG0180001043.01	MsG0580027169.01	0.812863	3.423006e-51	2.200721e-48
MsG0180002912.01	MsG0580027169.01	0.836447	9.307823e-57	1.173344e-53
MsG0180003409.01	MsG0580027169.01	0.820215	7.715268e-53	6.067087e-50
MsG0180003444.01	MsG0580027169.01	0.811661	6.261894e-51	3.898811e-48
MsG0180003669.01	MsG0580027169.01	0.830078	3.596044e-55	3.749176e-52
MsG0180003864.01	MsG0580027169.01	0.822954	1.797079e-53	1.526334e-50
MsG0180004060.01	MsG0580027169.01	0.811485	6.838001e-51	4.238134e-48
MsG0180004147.01	MsG0580027169.01	0.809843	1.548281e-50	9.188721e-48
MsG0180004162.01	MsG0580027169.01	0.807040	6.137012e-50	3.383061e-47
MsG0180004258.01	MsG0580027169.01	0.807288	5.439371e-50	3.017891e-47
MsG0180004320.01	MsG0580027169.01	0.811102	8.278821e-51	5.079530e-48
MsG0180004388.01	MsG0580027169.01	0.864077	1.576173e-64	4.886229e-61
MsG0180005125.01	MsG0580027169.01	0.814233	1.710129e-51	1.141126e-48
MsG0180005842.01	MsG0580027169.01	0.825910	3.622539e-54	3.346795e-51
MsG0180005860.01	MsG0580027169.01	0.821561	3.781363e-53	3.087033e-50
MsG0180005878.01	MsG0580027169.01	0.815588	8.558528e-52	5.925154e-49
MsG0180005944.01	MsG0580027169.01	0.803932	2.752347e-49	1.400907e-46
MsG0180005993.01	MsG0580027169.01	0.801049	1.081474e-48	5.114632e-46
MsG0180006016.01	MsG0580027169.01	0.838623	2.575503e-57	3.469378e-54
MsG0180006023.01	MsG0580027169.01	0.839811	1.267258e-57	1.770265e-54
MsG0180006069.01	MsG0580027169.01	0.818142	2.286606e-52	1.697697e-49
MsG0180006097.01	MsG0580027169.01	0.800898	1.161261e-48	5.470872e-46
MsG0180006226.01	MsG0580027169.01	-0.832611	8.564262e-56	9.621979e-53
MsG0180006235.01	MsG0580027169.01	0.825836	3.772889e-54	3.478193e-51
MsG0280006375.01	MsG0580027169.01	0.812290	4.566975e-51	2.892334e-48
MsG0280010551.01	MsG0580027169.01	0.812432	4.252343e-51	2.703443e-48
MsG0280010553.01	MsG0580027169.01	0.805569	1.253031e-49	6.651382e-47
MsG0280010614.01	MsG0580027169.01	0.830806	2.387138e-55	2.542460e-52
MsG0280010739.01	MsG0580027169.01	0.829562	4.802666e-55	4.932969e-52
MsG0280011070.01	MsG0580027169.01	0.828512	8.628242e-55	8.594670e-52
MsG0280011086.01	MsG0580027169.01	0.812351	4.430122e-51	2.810236e-48
MsG0280011141.01	MsG0580027169.01	0.806130	9.551581e-50	5.143818e-47
MsG0280011446.01	MsG0580027169.01	0.829056	6.373662e-55	6.449774e-52
MsG0380011754.01	MsG0580027169.01	0.802282	6.040452e-49	2.947293e-46
MsG0380012471.01	MsG0580027169.01	0.810105	1.359834e-50	8.125875e-48
MsG0380012528.01	MsG0580027169.01	0.810222	1.282982e-50	7.689433e-48
MsG0380014365.01	MsG0580027169.01	0.811750	5.987330e-51	3.736894e-48
MsG0380014573.01	MsG0580027169.01	0.825629	4.223975e-54	3.871168e-51
MsG0380014680.01	MsG0580027169.01	0.820080	8.284102e-53	6.490761e-50
MsG0480018637.01	MsG0580027169.01	0.820316	7.315004e-53	5.768404e-50
MsG0480018787.01	MsG0580027169.01	0.832618	8.531571e-56	9.587215e-53
MsG0480018970.01	MsG0580027169.01	0.842089	3.199224e-58	4.797887e-55
MsG0480019816.01	MsG0580027169.01	0.810811	9.572831e-51	5.828143e-48
MsG0480020065.01	MsG0580027169.01	0.809031	2.313181e-50	1.343414e-47
MsG0480020090.01	MsG0580027169.01	0.820824	5.592036e-53	4.472452e-50
MsG0480020445.01	MsG0580027169.01	0.836599	8.514727e-57	1.078168e-53
MsG0480020591.01	MsG0580027169.01	0.833696	4.598004e-56	5.335762e-53
MsG0480021554.01	MsG0580027169.01	0.809948	1.470054e-50	8.748427e-48
MsG0480021731.01	MsG0580027169.01	0.808351	3.231587e-50	1.843621e-47
MsG0480021953.01	MsG0580027169.01	0.843322	1.504420e-58	2.345516e-55
MsG0480022164.01	MsG0580027169.01	0.800376	1.483811e-48	6.898355e-46
MsG0480022480.01	MsG0580027169.01	0.873352	1.571215e-67	6.797909e-64
MsG0480022971.01	MsG0580027169.01	0.828779	7.434329e-55	7.462744e-52
MsG0480023088.01	MsG0580027169.01	0.834730	2.530815e-56	3.029300e-53
MsG0480023245.01	MsG0580027169.01	0.830168	3.419228e-55	3.574503e-52
MsG0480023517.01	MsG0580027169.01	0.805498	1.296705e-49	6.870700e-47
MsG0480023600.01	MsG0580027169.01	0.801431	9.033157e-49	4.313128e-46
MsG0480023707.01	MsG0580027169.01	0.860293	2.284423e-63	6.213025e-60
MsG0580024035.01	MsG0580027169.01	0.811684	6.191131e-51	3.857078e-48
MsG0580024073.01	MsG0580027169.01	0.801981	6.967948e-49	3.373506e-46
MsG0580024353.01	MsG0580027169.01	0.803677	3.110677e-49	1.572752e-46
MsG0580024374.01	MsG0580027169.01	0.812875	3.401747e-51	2.187835e-48
MsG0580024563.01	MsG0580027169.01	0.817297	3.548519e-52	2.574156e-49
MsG0580024733.01	MsG0580027169.01	0.823221	1.556752e-53	1.332209e-50
MsG0580025134.01	MsG0580027169.01	0.821356	4.217037e-53	3.422975e-50
MsG0580025321.01	MsG0580027169.01	0.821421	4.074044e-53	3.312971e-50
MsG0580025989.01	MsG0580027169.01	0.806252	9.001682e-50	4.862969e-47
MsG0580026023.01	MsG0580027169.01	0.800083	1.701941e-48	7.853717e-46
MsG0380015181.01	MsG0580027169.01	0.801060	1.075713e-48	5.088813e-46
MsG0380015779.01	MsG0580027169.01	0.866077	3.712189e-65	1.235193e-61
MsG0380015793.01	MsG0580027169.01	-0.802459	5.555384e-49	2.722749e-46
MsG0380016227.01	MsG0580027169.01	0.825469	4.609551e-54	4.205022e-51
MsG0380016581.01	MsG0580027169.01	0.812106	5.010064e-51	3.156999e-48
MsG0380016624.01	MsG0580027169.01	0.802029	6.811422e-49	3.301929e-46
MsG0380016859.01	MsG0580027169.01	0.820968	5.182040e-53	4.161506e-50
MsG0380016971.01	MsG0580027169.01	0.816507	5.335966e-52	3.788008e-49
MsG0380017040.01	MsG0580027169.01	0.805109	1.564569e-49	8.207685e-47
MsG0380017403.01	MsG0580027169.01	0.817066	3.998775e-52	2.882635e-49
MsG0380017604.01	MsG0580027169.01	0.801189	1.012504e-48	4.805130e-46
MsG0380017631.01	MsG0580027169.01	0.809544	1.795468e-50	1.057169e-47
MsG0380017998.01	MsG0580027169.01	0.824761	6.772948e-54	6.055935e-51
MsG0380018009.01	MsG0580027169.01	0.857134	2.007319e-62	4.904210e-59
MsG0480018126.01	MsG0580027169.01	0.820484	6.695357e-53	5.304342e-50
MsG0480018163.01	MsG0580027169.01	0.815168	1.061275e-51	7.262506e-49
MsG0480018206.01	MsG0580027169.01	0.818891	1.546608e-52	1.172367e-49
MsG0580026771.01	MsG0580027169.01	0.859176	4.958344e-63	1.297742e-59
MsG0580027129.01	MsG0580027169.01	0.808809	2.580202e-50	1.489630e-47
MsG0580027169.01	MsG0580027345.01	0.827845	1.248986e-54	1.220159e-51
MsG0580027169.01	MsG0580027951.01	0.812349	4.434595e-51	2.812929e-48
MsG0580027169.01	MsG0580029189.01	0.829041	6.424499e-55	6.498520e-52
MsG0580027169.01	MsG0580029762.01	0.815356	9.640034e-52	6.631493e-49
MsG0580027169.01	MsG0580030235.01	0.841871	3.652594e-58	5.440100e-55
MsG0580027169.01	MsG0680030316.01	0.811508	6.758764e-51	4.191637e-48
MsG0580027169.01	MsG0680030685.01	0.830711	2.517959e-55	2.674327e-52
MsG0580027169.01	MsG0680031651.01	0.839353	1.666667e-57	2.295800e-54
MsG0580027169.01	MsG0680033391.01	0.806224	9.126474e-50	4.926882e-47
MsG0580027169.01	MsG0680035346.01	0.805028	1.626401e-49	8.514169e-47
MsG0580027169.01	MsG0680035573.01	0.822693	2.066710e-53	1.742457e-50
MsG0580027169.01	MsG0680035777.01	0.823546	1.306777e-53	1.128571e-50
MsG0580027169.01	MsG0780036477.01	0.809304	2.021656e-50	1.182745e-47
MsG0580027169.01	MsG0780036609.01	0.800655	1.301949e-48	6.095712e-46
MsG0580027169.01	MsG0780037109.01	0.807766	4.304799e-50	2.418240e-47
MsG0580027169.01	MsG0780037515.01	0.801830	7.482993e-49	3.609284e-46
MsG0580027169.01	MsG0780038444.01	0.813599	2.359525e-51	1.547646e-48
MsG0580027169.01	MsG0780038495.01	0.851247	1.004641e-60	2.020975e-57
MsG0580027169.01	MsG0780038837.01	0.800508	1.394476e-48	6.504497e-46
MsG0580027169.01	MsG0780040442.01	0.830401	2.998814e-55	3.156050e-52
MsG0580027169.01	MsG0780040488.01	0.825751	3.951113e-54	3.633750e-51
MsG0580027169.01	MsG0780040861.01	0.857124	2.021124e-62	4.935899e-59
MsG0580027169.01	MsG0780041161.01	0.818593	1.808184e-52	1.359221e-49
MsG0580027169.01	MsG0780041399.01	0.802404	5.700124e-49	2.789932e-46
MsG0580027169.01	MsG0780041469.01	0.804593	2.005116e-49	1.037981e-46
MsG0580027169.01	MsG0780041559.01	0.801115	1.048604e-48	4.967216e-46
MsG0580027169.01	MsG0780041754.01	0.805561	1.257669e-49	6.674687e-47
MsG0580027169.01	MsG0880041883.01	0.831476	1.633442e-55	1.774678e-52
MsG0580027169.01	MsG0880041969.01	0.803785	2.953568e-49	1.497559e-46
MsG0580027169.01	MsG0880042232.01	0.804939	1.698235e-49	8.870568e-47
MsG0580027169.01	MsG0880042543.01	0.813156	2.951617e-51	1.912660e-48
MsG0580027169.01	MsG0880042811.01	0.823396	1.416718e-53	1.218245e-50
MsG0580027169.01	MsG0880043491.01	0.805004	1.645472e-49	8.608859e-47
MsG0580027169.01	MsG0880043547.01	0.805584	1.243852e-49	6.605456e-47
MsG0580027169.01	MsG0880044415.01	0.807002	6.251152e-50	3.442582e-47
MsG0580027169.01	MsG0880045290.01	0.802949	4.400526e-49	2.183778e-46
MsG0580027169.01	MsG0880045653.01	0.820337	7.233051e-53	5.707161e-50
MsG0580027169.01	MsG0880046012.01	0.822090	2.852699e-53	2.364009e-50
MsG0580027169.01	MsG0880046113.01	0.802886	4.534501e-49	2.246747e-46
MsG0580027169.01	MsG0880046479.01	0.816203	6.240102e-52	4.393505e-49
MsG0580027169.01	MsG0880046669.01	0.834028	3.797650e-56	4.451572e-53
MsG0580027169.01	MsG0880046973.01	0.800469	1.420084e-48	6.617421e-46
MsG0580027169.01	MsG0880047120.01	0.806159	9.419385e-50	5.076447e-47
MsG0580027169.01	MsG0880047191.01	0.820952	5.226251e-53	4.195111e-50
MsG0580027169.01	MsG0880047497.01	0.825653	4.168509e-54	3.822947e-51
MsG0280006595.01	MsG0580027169.01	0.828118	1.074036e-54	1.057689e-51
MsG0280007071.01	MsG0580027169.01	0.803321	3.686053e-49	1.846707e-46
MsG0280007088.01	MsG0580027169.01	0.826205	3.082374e-54	2.871669e-51
MsG0280007089.01	MsG0580027169.01	0.833139	6.330281e-56	7.225764e-53
MsG0280007609.01	MsG0580027169.01	0.875368	3.255269e-68	1.515934e-64
MsG0280007610.01	MsG0580027169.01	0.805366	1.381776e-49	7.296955e-47
MsG0280007815.01	MsG0580027169.01	0.806745	7.084910e-50	3.875845e-47
MsG0280008885.01	MsG0580027169.01	0.803465	3.441783e-49	1.730758e-46
MsG0280008907.01	MsG0580027169.01	0.812704	3.708892e-51	2.374725e-48
MsG0280008971.01	MsG0580027169.01	0.835711	1.431144e-56	1.764555e-53
MsG0280009140.01	MsG0580027169.01	0.812545	4.017830e-51	2.561921e-48
MsG0280010389.01	MsG0580027169.01	0.846246	2.450057e-59	4.190692e-56
MsG0280010416.01	MsG0580027169.01	0.809495	1.839652e-50	1.081736e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027169.01.T01	MTR_4g105330	93.333	225	12	1	1	222	78	302	1.28e-153	428

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027169.01.T01	AT3G48210	37.991	229	126	6	1	222	93	312	4.35e-40	140

Find 77 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTCTTGCTTTGGCTTTCC+CGG	0.204609	5:-55219765	None:intergenic
TGGCCATCTCTTGGTATAAC+AGG	0.291680	5:+55222874	MsG0580027169.01.T01:CDS
CATCTGTCTTGTTTAATTCA+TGG	0.302544	5:-55223235	None:intergenic
CCCGGAGTGTCCAAAGTAAT+AGG	0.302619	5:-55219747	None:intergenic
TTTGATGGATTGGAAGGATT+TGG	0.310983	5:-55220981	None:intergenic
CGAGTTATATTCTTGTTTCC+TGG	0.325896	5:-55221373	None:intergenic
GTAAAAGAGTACTCCTCATT+TGG	0.339017	5:-55223054	None:intergenic
GAAGGAAGTGGAAGATGAAT+TGG	0.348717	5:+55221145	MsG0580027169.01.T01:five_prime_UTR
GTTGGTTTCTTTGACGGCTT+CGG	0.351708	5:-55219711	None:intergenic
TCATTTGTTGGTTTCTTTGA+CGG	0.368767	5:-55219717	None:intergenic
CTTCGATGAGCTCATGTCTA+AGG	0.376845	5:+55219686	None:intergenic
TCGCCAGTCTCCACGTTTGA+AGG	0.380013	5:+55223945	MsG0580027169.01.T01:CDS
TCTTGGTATAACAGGGTACT+TGG	0.397967	5:+55222882	MsG0580027169.01.T01:CDS
TGAGCACAATCCTATTACTT+TGG	0.401066	5:+55219737	MsG0580027169.01.T01:five_prime_UTR
TTCTGATTCGCAGTGGGTTG+AGG	0.409859	5:-55220912	None:intergenic
ATAACTTCGATCTCTTGCTT+TGG	0.412864	5:-55219774	None:intergenic
CGAACAGATGAAGCAGAATC+AGG	0.420353	5:-55223925	None:intergenic
AGTTCGCAGAAGGAAAAGTT+TGG	0.432380	5:+55223891	MsG0580027169.01.T01:CDS
TCCTATTACTTTGGACACTC+CGG	0.433313	5:+55219746	MsG0580027169.01.T01:five_prime_UTR
TCATCCGCTCCTGGCACATC+TGG	0.434205	5:+55223790	MsG0580027169.01.T01:CDS
AGGAGCGGATGAAGATATAA+AGG	0.452608	5:-55223779	None:intergenic
GTTCTAACAACAGTTAATCC+TGG	0.455209	5:+55223748	MsG0580027169.01.T01:CDS
TTTATATCTTCATCCGCTCC+TGG	0.466324	5:+55223781	MsG0580027169.01.T01:CDS
TTCAGAAAAGACGCGAAAAG+AGG	0.467224	5:+55221266	MsG0580027169.01.T01:intron
AGAGGTTAAACATAAATTGA+AGG	0.467743	5:+55221127	MsG0580027169.01.T01:five_prime_UTR
GGCTAGGCGAATGGCTCTGT+TGG	0.468116	5:+55221287	MsG0580027169.01.T01:exon
TAAACATAAATTGAAGGAAG+TGG	0.475512	5:+55221133	MsG0580027169.01.T01:five_prime_UTR
CATTGTGAACCTTCCTTCGA+TGG	0.475570	5:+55223197	MsG0580027169.01.T01:CDS
ATAGGATTGTGCTCATTTGT+TGG	0.477823	5:-55219729	None:intergenic
AAAGCAAGAGATCGAAGTTA+TGG	0.486389	5:+55219776	MsG0580027169.01.T01:five_prime_UTR
AAGTGGAACGCTCTGGCAGT+AGG	0.491247	5:+55223848	MsG0580027169.01.T01:CDS
AAACACAGGTGGCCATCTCT+TGG	0.495412	5:+55222865	MsG0580027169.01.T01:CDS
GCTTCTGCTAAGGCTAGAGT+TGG	0.508997	5:+55221318	MsG0580027169.01.T01:CDS
CTTCGATGGTATTGAAGAGC+TGG	0.509662	5:+55223211	MsG0580027169.01.T01:CDS
ATACCTTCAAACGTGGAGAC+TGG	0.510056	5:-55223948	None:intergenic
CCTATTACTTTGGACACTCC+GGG	0.512175	5:+55219747	MsG0580027169.01.T01:five_prime_UTR
GGATGCTATTGCTTCTGCTA+AGG	0.514115	5:+55221308	MsG0580027169.01.T01:CDS
GGATTGGAAGGATTTGGAAA+AGG	0.514441	5:-55220975	None:intergenic
CAACAAACCGCGAAAACTCA+AGG	0.515409	5:+55221013	MsG0580027169.01.T01:five_prime_UTR
TTAAGTTTGTCAGAGTAATG+AGG	0.518116	5:+55223264	MsG0580027169.01.T01:CDS
CTTCTCAGTTCAATTAGAAG+AGG	0.527370	5:+55221109	MsG0580027169.01.T01:five_prime_UTR
TTTCTATCGCAGCAATCACT+TGG	0.529798	5:+55221396	MsG0580027169.01.T01:CDS
GAGCATGAAGTGGAACGCTC+TGG	0.532714	5:+55223841	MsG0580027169.01.T01:CDS
ATCTGAAGGGACGTTGAATG+AGG	0.533325	5:+55222824	MsG0580027169.01.T01:CDS
GATATAAAGGCAGATTCTCC+AGG	0.534729	5:-55223766	None:intergenic
TTTCAACTCAAGTATTCAAG+AGG	0.534821	5:+55221344	MsG0580027169.01.T01:CDS
GAAGCTTTGGCAGCATCTGA+AGG	0.537582	5:+55222810	MsG0580027169.01.T01:intron
GAAGGATTTGGAAAAGGAAG+CGG	0.548779	5:-55220969	None:intergenic
GAGTGAGGAGAACAGAAAGA+TGG	0.553308	5:+55220877	MsG0580027169.01.T01:intron
TGTGATTTCTGATTCGCAGT+GGG	0.560061	5:-55220918	None:intergenic
CTCTTCTAATTGAACTGAGA+AGG	0.563595	5:-55221108	None:intergenic
TGATTCGCAGTGGGTTGAGG+AGG	0.566194	5:-55220909	None:intergenic
TAGAAGTGATTCTACAGCCA+TGG	0.573031	5:+55223813	MsG0580027169.01.T01:CDS
CGGCGAAAGCGTCGACGGTG+TGG	0.579854	5:-55220949	None:intergenic
GAATTGGTTAAAGTACTCGC+AGG	0.581617	5:+55221161	MsG0580027169.01.T01:five_prime_UTR
GGAAGCGGCGAAAGCGTCGA+CGG	0.585423	5:-55220954	None:intergenic
AAGCTTTGGCAGCATCTGAA+GGG	0.586020	5:+55222811	MsG0580027169.01.T01:intron
TCACTTCTACCAGATGTGCC+AGG	0.586982	5:-55223799	None:intergenic
AGTGGAACGCTCTGGCAGTA+GGG	0.587890	5:+55223849	MsG0580027169.01.T01:CDS
GCGAAAAGAGGCTAGGCGAA+TGG	0.596267	5:+55221278	MsG0580027169.01.T01:exon
TTTCAAGAAGCATTGGCACA+AGG	0.601367	5:+55223293	MsG0580027169.01.T01:CDS
TCTGAAGGGACGTTGAATGA+GGG	0.602292	5:+55222825	MsG0580027169.01.T01:CDS
TACCCTGTTATACCAAGAGA+TGG	0.604810	5:-55222877	None:intergenic
AAAAGACGCGAAAAGAGGCT+AGG	0.609214	5:+55221271	MsG0580027169.01.T01:five_prime_UTR
CAGCTCTTCAATACCATCGA+AGG	0.622116	5:-55223210	None:intergenic
AAATTTGAAGAATCCAAATG+AGG	0.623545	5:+55223041	MsG0580027169.01.T01:CDS
CATGAATTAAACAAGACAGA+TGG	0.623903	5:+55223236	MsG0580027169.01.T01:CDS
TCTTCAATACCATCGAAGGA+AGG	0.637490	5:-55223206	None:intergenic
CATGGAAAATGAGCATGAAG+TGG	0.637509	5:+55223831	MsG0580027169.01.T01:CDS
GTATTCAAGAGGTTCGAACC+AGG	0.644559	5:+55221355	MsG0580027169.01.T01:CDS
GTGTGATTTCTGATTCGCAG+TGG	0.646914	5:-55220919	None:intergenic
GGCCATCTCTTGGTATAACA+GGG	0.647513	5:+55222875	MsG0580027169.01.T01:CDS
TCTACCAGATGTGCCAGGAG+CGG	0.654573	5:-55223794	None:intergenic
TTTCGCGGTTTGTTGTGCGG+TGG	0.664534	5:-55221005	None:intergenic
TGAACATACAGATGAAACAC+AGG	0.685192	5:+55222851	MsG0580027169.01.T01:CDS
GTAATTCATACCTTCAAACG+TGG	0.717846	5:-55223955	None:intergenic
ACATACAGATGAAACACAGG+TGG	0.719890	5:+55222854	MsG0580027169.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATTTAAAAAGATTGA+TGG	-	Chr5:55222279-55222298	None:intergenic	10.0%
!!	ATATAATATTAGTTACATAT+TGG	-	Chr5:55219916-55219935	None:intergenic	10.0%
!!!	AATGATTTTTTTTTTTTTTG+AGG	+	Chr5:55220706-55220725	MsG0580027169.01.T01:intron	10.0%
!!!	ATGATTTTTTTTTTTTTTGA+GGG	+	Chr5:55220707-55220726	MsG0580027169.01.T01:intron	10.0%
!!	AAATATCAAAATTACACAAT+CGG	-	Chr5:55219889-55219908	None:intergenic	15.0%
!!	TAAGTGTGTTTAAATTATTA+CGG	+	Chr5:55219964-55219983	MsG0580027169.01.T01:intron	15.0%
!!	TGTAATAGTCATTATTTAAA+TGG	+	Chr5:55220672-55220691	MsG0580027169.01.T01:intron	15.0%
!!	TGTTATAAAATAAGATTTGA+AGG	+	Chr5:55221995-55222014	MsG0580027169.01.T01:intron	15.0%
!!!	AATTAACTTTTTTTTGCTTA+AGG	+	Chr5:55222937-55222956	MsG0580027169.01.T01:intron	15.0%
!!!	ATGTATAAGTATTTTTATCA+TGG	-	Chr5:55223086-55223105	None:intergenic	15.0%
!!!	TCATTATAATATGAAGTATT+TGG	-	Chr5:55223468-55223487	None:intergenic	15.0%
!!!	TTCAAATCTTATTTTATAAC+AGG	-	Chr5:55221996-55222015	None:intergenic	15.0%
!!!	TTGTGTAATTTTGATATTTA+TGG	+	Chr5:55219890-55219909	MsG0580027169.01.T01:intron	15.0%
!!	AAAAATTTCATTAAATGCAG+TGG	-	Chr5:55220198-55220217	None:intergenic	20.0%
!!	AAAATCACAATATTTGAGTA+AGG	+	Chr5:55220618-55220637	MsG0580027169.01.T01:intron	20.0%
!!	AAATCACAATATTTGAGTAA+GGG	+	Chr5:55220619-55220638	MsG0580027169.01.T01:intron	20.0%
!!	AAATTTAAAAAGATTGATGG+TGG	-	Chr5:55222276-55222295	None:intergenic	20.0%
!!	AAGTGTGTTTAAATTATTAC+GGG	+	Chr5:55219965-55219984	MsG0580027169.01.T01:intron	20.0%
!!	GGGTATATAAATTAATGAAT+TGG	+	Chr5:55220727-55220746	MsG0580027169.01.T01:intron	20.0%
!!	GTTCATACATTCAAAAATAA+TGG	-	Chr5:55222512-55222531	None:intergenic	20.0%
!!	TAAAGTTGTAATGAAGTAAT+GGG	+	Chr5:55222322-55222341	MsG0580027169.01.T01:intron	20.0%
!!	TTAAAGTTGTAATGAAGTAA+TGG	+	Chr5:55222321-55222340	MsG0580027169.01.T01:intron	20.0%
!!	TTAATAGAATCACTTCATAT+TGG	-	Chr5:55223428-55223447	None:intergenic	20.0%
!!!	AGCTTATTTGTTTTAAGTTA+GGG	+	Chr5:55221211-55221230	MsG0580027169.01.T01:intron	20.0%
!!!	ATCTTCAAAAATGATTTTAC+AGG	-	Chr5:55221963-55221982	None:intergenic	20.0%
!!!	TAGCTTATTTGTTTTAAGTT+AGG	+	Chr5:55221210-55221229	MsG0580027169.01.T01:intron	20.0%
!	AAAATGAAAACCCTAATAAG+AGG	-	Chr5:55221081-55221100	None:intergenic	25.0%
!	AAATTGTTTCAATCTAATGC+AGG	-	Chr5:55220522-55220541	None:intergenic	25.0%
!	AAATTTGAAGAATCCAAATG+AGG	+	Chr5:55223041-55223060	MsG0580027169.01.T01:CDS	25.0%
!	ACACTGAAAAATAAAAGAGA+AGG	-	Chr5:55223175-55223194	None:intergenic	25.0%
!	AGAGGTTAAACATAAATTGA+AGG	+	Chr5:55221127-55221146	MsG0580027169.01.T01:five_prime_UTR	25.0%
!	AGTGGTAGAATTAATGAAAT+TGG	+	Chr5:55222372-55222391	MsG0580027169.01.T01:intron	25.0%
!	ATTATTGAACTGTTCAAACA+TGG	+	Chr5:55223545-55223564	MsG0580027169.01.T01:intron	25.0%
!	CTCATGAAATTCAAAAACAT+TGG	+	Chr5:55220850-55220869	MsG0580027169.01.T01:intron	25.0%
!	GCTTAAATAGCTATAAATTG+CGG	-	Chr5:55221448-55221467	None:intergenic	25.0%
!	GTATATAAAATCATAAACGC+AGG	-	Chr5:55223117-55223136	None:intergenic	25.0%
!	GTGGTAGAATTAATGAAATT+GGG	+	Chr5:55222373-55222392	MsG0580027169.01.T01:intron	25.0%
!	GTTCAATAATACATATGAAC+TGG	-	Chr5:55223535-55223554	None:intergenic	25.0%
!	GTTCATACATTCAAAAACAA+TGG	-	Chr5:55222467-55222486	None:intergenic	25.0%
!	TAAACATAAATTGAAGGAAG+TGG	+	Chr5:55221133-55221152	MsG0580027169.01.T01:five_prime_UTR	25.0%
!!	AAAAATAAAAGAGAAGGTGT+AGG	-	Chr5:55223169-55223188	None:intergenic	25.0%
!!	GAAAAAATTTCAAGAAGCAT+TGG	+	Chr5:55223286-55223305	MsG0580027169.01.T01:CDS	25.0%
!!	GATCTAATTGCTATTAAACA+GGG	+	Chr5:55223719-55223738	MsG0580027169.01.T01:intron	25.0%
!!	TGATCTAATTGCTATTAAAC+AGG	+	Chr5:55223718-55223737	MsG0580027169.01.T01:intron	25.0%
!!	TTACTCTTTTCCTCTTATTA+GGG	+	Chr5:55221068-55221087	MsG0580027169.01.T01:intron	25.0%
!!	TTTACTCTTTTCCTCTTATT+AGG	+	Chr5:55221067-55221086	MsG0580027169.01.T01:intron	25.0%
!!	TTTTAATCCATATGAAGCTT+TGG	+	Chr5:55222797-55222816	MsG0580027169.01.T01:intron	25.0%
!!!	AAGGATGTTTTTTCTAACAA+TGG	+	Chr5:55222014-55222033	MsG0580027169.01.T01:intron	25.0%
!!!	AGGATGTTTTTTCTAACAAT+GGG	+	Chr5:55222015-55222034	MsG0580027169.01.T01:intron	25.0%
!!!	GCTTATTTGTTTTAAGTTAG+GGG	+	Chr5:55221212-55221231	MsG0580027169.01.T01:intron	25.0%
!!!	TGCATGAACTATTTTTCATA+AGG	+	Chr5:55221700-55221719	MsG0580027169.01.T01:intron	25.0%
	AAAAATAAGCCAATCCAAAC+GGG	-	Chr5:55221531-55221550	None:intergenic	30.0%
	AAACTGAACCAATAAGATGT+TGG	+	Chr5:55220493-55220512	MsG0580027169.01.T01:intron	30.0%
	AAGCTTGTCTTGTACAAAAT+CGG	+	Chr5:55222404-55222423	MsG0580027169.01.T01:intron	30.0%
	CAAAAATAAGCCAATCCAAA+CGG	-	Chr5:55221532-55221551	None:intergenic	30.0%
	CATGAATTAAACAAGACAGA+TGG	+	Chr5:55223236-55223255	MsG0580027169.01.T01:CDS	30.0%
	CGTCAGTTTAAGAAGTTTAT+CGG	-	Chr5:55220103-55220122	None:intergenic	30.0%
	CTCAAAATACTTATGCAAGA+TGG	-	Chr5:55221815-55221834	None:intergenic	30.0%
	CTTATTAGTTATGCTCTTCA+GGG	+	Chr5:55222995-55223014	MsG0580027169.01.T01:intron	30.0%
	CTTCTTAAACTGACGTAATT+TGG	+	Chr5:55220108-55220127	MsG0580027169.01.T01:intron	30.0%
	GCATAGTTTAATAGAGAAAC+TGG	+	Chr5:55220793-55220812	MsG0580027169.01.T01:intron	30.0%
	GTAAAAAATAAGCCAATCCA+TGG	+	Chr5:55221722-55221741	MsG0580027169.01.T01:intron	30.0%
	TAAATAGTATCTTCGCTTGT+TGG	+	Chr5:55223658-55223677	MsG0580027169.01.T01:intron	30.0%
	TACCACTTTGATACACAAAA+GGG	-	Chr5:55222359-55222378	None:intergenic	30.0%
	TCACACATAAATGATCCAAA+CGG	-	Chr5:55220020-55220039	None:intergenic	30.0%
	TCTTATTCATAAGCTTGTCA+AGG	+	Chr5:55221584-55221603	MsG0580027169.01.T01:intron	30.0%
	TGGATTAAAAGAGAAGATGT+GGG	-	Chr5:55222787-55222806	None:intergenic	30.0%
	TTAAGCTTGTAACTATGTTG+TGG	+	Chr5:55222120-55222139	MsG0580027169.01.T01:intron	30.0%
	TTAAGTTTGTCAGAGTAATG+AGG	+	Chr5:55223264-55223283	MsG0580027169.01.T01:CDS	30.0%
	TTATTAGTTATGCTCTTCAG+GGG	+	Chr5:55222996-55223015	MsG0580027169.01.T01:intron	30.0%
	TTGAGATTACATGTACTATG+AGG	+	Chr5:55221830-55221849	MsG0580027169.01.T01:intron	30.0%
	TTGTTGGAAATTGGATGAAA+GGG	+	Chr5:55223674-55223693	MsG0580027169.01.T01:intron	30.0%
	TTTCAACTCAAGTATTCAAG+AGG	+	Chr5:55221344-55221363	MsG0580027169.01.T01:CDS	30.0%
!	AGATAGGTTATGACACTTTT+GGG	+	Chr5:55219836-55219855	MsG0580027169.01.T01:intron	30.0%
!	ATGGATTAAAAGAGAAGATG+TGG	-	Chr5:55222788-55222807	None:intergenic	30.0%
!	CTCACTGGTTATACAAATTA+AGG	+	Chr5:55220567-55220586	MsG0580027169.01.T01:intron	30.0%
!	TAGATAGGTTATGACACTTT+TGG	+	Chr5:55219835-55219854	MsG0580027169.01.T01:intron	30.0%
!	TATCACTAGTCTAGTTAGAT+AGG	+	Chr5:55219820-55219839	MsG0580027169.01.T01:intron	30.0%
!!	CATCTGTCTTGTTTAATTCA+TGG	-	Chr5:55223238-55223257	None:intergenic	30.0%
!!	GCAATTTGGTCTTTTACTTT+GGG	+	Chr5:55220411-55220430	MsG0580027169.01.T01:intron	30.0%
!!	GTAAATCTCTGTTTTTCTTC+TGG	+	Chr5:55221901-55221920	MsG0580027169.01.T01:intron	30.0%
!!	TACTTGGTTTTCATGTCAAA+GGG	+	Chr5:55222898-55222917	MsG0580027169.01.T01:CDS	30.0%
!!	TCATTTGTTGGTTTCTTTGA+CGG	-	Chr5:55219720-55219739	None:intergenic	30.0%
!!	TTATTGGTTTACTACATCTC+TGG	+	Chr5:55222167-55222186	MsG0580027169.01.T01:intron	30.0%
!!!	TGCATTCTATGTTTTGATTG+AGG	+	Chr5:55222643-55222662	MsG0580027169.01.T01:intron	30.0%
	AAAAACATTGGATACGAGTG+AGG	+	Chr5:55220862-55220881	MsG0580027169.01.T01:intron	35.0%
	AAACACAATGTGATTCTCAC+TGG	+	Chr5:55220552-55220571	MsG0580027169.01.T01:intron	35.0%
	AAAGCAAGAGATCGAAGTTA+TGG	+	Chr5:55219776-55219795	MsG0580027169.01.T01:five_prime_UTR	35.0%
	AATTATGATGTCGACAACCA+TGG	-	Chr5:55221742-55221761	None:intergenic	35.0%
	AATTCTGCTCTTTACCCAAA+TGG	-	Chr5:55220448-55220467	None:intergenic	35.0%
	ACCAGCGAATTTGTGATATT+TGG	+	Chr5:55219993-55220012	MsG0580027169.01.T01:intron	35.0%
	ACCATAGAACTAGTAAGACA+TGG	+	Chr5:55221925-55221944	MsG0580027169.01.T01:intron	35.0%
	AGAAAAAACAAGTTCGCAGA+AGG	+	Chr5:55223881-55223900	MsG0580027169.01.T01:CDS	35.0%
	AGCATAACTTAACATGCGTA+GGG	+	Chr5:55220388-55220407	MsG0580027169.01.T01:intron	35.0%
	AGCTTGTCTTGTACAAAATC+GGG	+	Chr5:55222405-55222424	MsG0580027169.01.T01:intron	35.0%
	ATAACTTCGATCTCTTGCTT+TGG	-	Chr5:55219777-55219796	None:intergenic	35.0%
	ATACAGATTCACACTCTGTT+TGG	+	Chr5:55221479-55221498	MsG0580027169.01.T01:intron	35.0%
	ATAGGATTGTGCTCATTTGT+TGG	-	Chr5:55219732-55219751	None:intergenic	35.0%
	ATATTGGAGCTCTGCATATT+TGG	-	Chr5:55223412-55223431	None:intergenic	35.0%
	ATGGTTGTCGACATCATAAT+TGG	+	Chr5:55221741-55221760	MsG0580027169.01.T01:intron	35.0%
	ATTAAAAGAGAAGATGTGGG+AGG	-	Chr5:55222784-55222803	None:intergenic	35.0%
	CCTTATTAGTTATGCTCTTC+AGG	+	Chr5:55222994-55223013	MsG0580027169.01.T01:intron	35.0%
	CTACCACTTTGATACACAAA+AGG	-	Chr5:55222360-55222379	None:intergenic	35.0%
	CTCTTCTAATTGAACTGAGA+AGG	-	Chr5:55221111-55221130	None:intergenic	35.0%
	CTTCTCAGTTCAATTAGAAG+AGG	+	Chr5:55221109-55221128	MsG0580027169.01.T01:five_prime_UTR	35.0%
	CTTGTTGGAAATTGGATGAA+AGG	+	Chr5:55223673-55223692	MsG0580027169.01.T01:intron	35.0%
	GTTCTAACAACAGTTAATCC+TGG	+	Chr5:55223748-55223767	MsG0580027169.01.T01:CDS	35.0%
	TACTTATGCAAGATGGCATT+TGG	-	Chr5:55221808-55221827	None:intergenic	35.0%
	TAGCATAACTTAACATGCGT+AGG	+	Chr5:55220387-55220406	MsG0580027169.01.T01:intron	35.0%
	TATGAATGTGACGCGATTAT+GGG	+	Chr5:55220137-55220156	MsG0580027169.01.T01:intron	35.0%
	TCCAAATATCACAAATTCGC+TGG	-	Chr5:55219997-55220016	None:intergenic	35.0%
	TGAACATACAGATGAAACAC+AGG	+	Chr5:55222851-55222870	MsG0580027169.01.T01:CDS	35.0%
	TGAGCACAATCCTATTACTT+TGG	+	Chr5:55219737-55219756	MsG0580027169.01.T01:five_prime_UTR	35.0%
	TTAACTCACACTAGTGCAAA+TGG	+	Chr5:55220238-55220257	MsG0580027169.01.T01:intron	35.0%
	TTATGAATGTGACGCGATTA+TGG	+	Chr5:55220136-55220155	MsG0580027169.01.T01:intron	35.0%
	TTTGTGATATTTGGACCGTT+TGG	+	Chr5:55220002-55220021	MsG0580027169.01.T01:intron	35.0%
!	ATGAAGTAATGGGATATCGT+TGG	+	Chr5:55222332-55222351	MsG0580027169.01.T01:intron	35.0%
!	CCTGAAGAGCATAACTAATA+AGG	-	Chr5:55222997-55223016	None:intergenic	35.0%
!	CGAGTTATATTCTTGTTTCC+TGG	-	Chr5:55221376-55221395	None:intergenic	35.0%
!	TAACTATGTTGTGGTCGTTT+TGG	+	Chr5:55222129-55222148	MsG0580027169.01.T01:intron	35.0%
!	TATCTTCGCTTGTTGGAAAT+TGG	+	Chr5:55223665-55223684	MsG0580027169.01.T01:intron	35.0%
!!	AACTATGTTGTGGTCGTTTT+GGG	+	Chr5:55222130-55222149	MsG0580027169.01.T01:intron	35.0%
!!	ACTTGGTTTTCATGTCAAAG+GGG	+	Chr5:55222899-55222918	MsG0580027169.01.T01:CDS	35.0%
!!	GGCAATTTGGTCTTTTACTT+TGG	+	Chr5:55220410-55220429	MsG0580027169.01.T01:intron	35.0%
!!	GTAAAAGAGTACTCCTCATT+TGG	-	Chr5:55223057-55223076	None:intergenic	35.0%
!!	GTACTTGGTTTTCATGTCAA+AGG	+	Chr5:55222897-55222916	MsG0580027169.01.T01:CDS	35.0%
!!	TCCATGTCTTACTAGTTCTA+TGG	-	Chr5:55221929-55221948	None:intergenic	35.0%
!!	TTTGATGGATTGGAAGGATT+TGG	-	Chr5:55220984-55221003	None:intergenic	35.0%
	AAAAAGCTCCAACGTTGTTG+TGG	-	Chr5:55220823-55220842	None:intergenic	40.0%
	ACATACAGATGAAACACAGG+TGG	+	Chr5:55222854-55222873	MsG0580027169.01.T01:CDS	40.0%
	AGGAGCGGATGAAGATATAA+AGG	-	Chr5:55223782-55223801	None:intergenic	40.0%
	AGTTCGCAGAAGGAAAAGTT+TGG	+	Chr5:55223891-55223910	MsG0580027169.01.T01:CDS	40.0%
	ATCTCAACCATGTGACTTCT+CGG	+	Chr5:55222218-55222237	MsG0580027169.01.T01:intron	40.0%
	ATGAGTGTATGCATGTGATG+TGG	+	Chr5:55223342-55223361	MsG0580027169.01.T01:intron	40.0%
	CATGGAAAATGAGCATGAAG+TGG	+	Chr5:55223831-55223850	MsG0580027169.01.T01:CDS	40.0%
	GAAGGAAGTGGAAGATGAAT+TGG	+	Chr5:55221145-55221164	MsG0580027169.01.T01:five_prime_UTR	40.0%
	GAAGGATTTGGAAAAGGAAG+CGG	-	Chr5:55220972-55220991	None:intergenic	40.0%
	GATATAAAGGCAGATTCTCC+AGG	-	Chr5:55223769-55223788	None:intergenic	40.0%
	GCATAACTTAACATGCGTAG+GGG	+	Chr5:55220389-55220408	MsG0580027169.01.T01:intron	40.0%
	GCGAAAACTCAAGGTCTTTA+GGG	+	Chr5:55221022-55221041	MsG0580027169.01.T01:intron	40.0%
	GCGGCATTTACATGAAAAAG+AGG	-	Chr5:55221429-55221448	None:intergenic	40.0%
	GGATTGGAAGGATTTGGAAA+AGG	-	Chr5:55220978-55220997	None:intergenic	40.0%
	GGGAATGAGCAAAACCATTT+GGG	+	Chr5:55220431-55220450	MsG0580027169.01.T01:intron	40.0%
	GGTTGAGATTCTCTCTTCAA+TGG	-	Chr5:55222207-55222226	None:intergenic	40.0%
	GTCGACATCATAATTGGACA+TGG	+	Chr5:55221747-55221766	MsG0580027169.01.T01:intron	40.0%
	TACACAATCGGCCACAAAAA+AGG	-	Chr5:55219877-55219896	None:intergenic	40.0%
	TACCCTGTTATACCAAGAGA+TGG	-	Chr5:55222880-55222899	None:intergenic	40.0%
	TACTATGAGGTTGTCATGTC+TGG	+	Chr5:55221843-55221862	MsG0580027169.01.T01:intron	40.0%
	TAGAAGTGATTCTACAGCCA+TGG	+	Chr5:55223813-55223832	MsG0580027169.01.T01:CDS	40.0%
	TAGCGTATGTTAACATGCGT+TGG	-	Chr5:55220295-55220314	None:intergenic	40.0%
	TCCTATTACTTTGGACACTC+CGG	+	Chr5:55219746-55219765	MsG0580027169.01.T01:five_prime_UTR	40.0%
	TCTTCAATACCATCGAAGGA+AGG	-	Chr5:55223209-55223228	None:intergenic	40.0%
	TGAGTGTATGCATGTGATGT+GGG	+	Chr5:55223343-55223362	MsG0580027169.01.T01:intron	40.0%
	TGGGAATGAGCAAAACCATT+TGG	+	Chr5:55220430-55220449	MsG0580027169.01.T01:intron	40.0%
	TTCAGAAAAGACGCGAAAAG+AGG	+	Chr5:55221266-55221285	MsG0580027169.01.T01:intron	40.0%
	TTTATATCTTCATCCGCTCC+TGG	+	Chr5:55223781-55223800	MsG0580027169.01.T01:CDS	40.0%
	TTTCTATCGCAGCAATCACT+TGG	+	Chr5:55221396-55221415	MsG0580027169.01.T01:CDS	40.0%
!	ATTCACACTCTGTTTGGTGA+AGG	+	Chr5:55221485-55221504	MsG0580027169.01.T01:intron	40.0%
!	CACTTCATGCTCATTTTCCA+TGG	-	Chr5:55223833-55223852	None:intergenic	40.0%
!	CGGGAGATTTTATGTTTGAC+TGG	+	Chr5:55222424-55222443	MsG0580027169.01.T01:intron	40.0%
!	CTAAAGACCTTGAGTTTTCG+CGG	-	Chr5:55221023-55221042	None:intergenic	40.0%
!	GAATTGGTTAAAGTACTCGC+AGG	+	Chr5:55221161-55221180	MsG0580027169.01.T01:five_prime_UTR	40.0%
!	GTCCCTTTTGTGTATCAAAG+TGG	+	Chr5:55222354-55222373	MsG0580027169.01.T01:intron	40.0%
!	TCTTGGTATAACAGGGTACT+TGG	+	Chr5:55222882-55222901	MsG0580027169.01.T01:CDS	40.0%
!	TGTGATTTCTGATTCGCAGT+GGG	-	Chr5:55220921-55220940	None:intergenic	40.0%
!!	ACTATGTTGTGGTCGTTTTG+GGG	+	Chr5:55222131-55222150	MsG0580027169.01.T01:intron	40.0%
!!	AGGTTATGACACTTTTGGGT+CGG	+	Chr5:55219840-55219859	MsG0580027169.01.T01:intron	40.0%
!!	CTTGGTTTTCATGTCAAAGG+GGG	+	Chr5:55222900-55222919	MsG0580027169.01.T01:CDS	40.0%
!!	TCGCAGGTTGGTTTGTATTA+GGG	+	Chr5:55221177-55221196	MsG0580027169.01.T01:intron	40.0%
!!	TTTCAAGAAGCATTGGCACA+AGG	+	Chr5:55223293-55223312	MsG0580027169.01.T01:CDS	40.0%
	AAAAGACGCGAAAAGAGGCT+AGG	+	Chr5:55221271-55221290	MsG0580027169.01.T01:five_prime_UTR	45.0%
	ATACCTTCAAACGTGGAGAC+TGG	-	Chr5:55223951-55223970	None:intergenic	45.0%
	ATCTGAAGGGACGTTGAATG+AGG	+	Chr5:55222824-55222843	MsG0580027169.01.T01:CDS	45.0%
	CAACAAACCGCGAAAACTCA+AGG	+	Chr5:55221013-55221032	MsG0580027169.01.T01:five_prime_UTR	45.0%
	CAGCTCTTCAATACCATCGA+AGG	-	Chr5:55223213-55223232	None:intergenic	45.0%
	CATTGTGAACCTTCCTTCGA+TGG	+	Chr5:55223197-55223216	MsG0580027169.01.T01:CDS	45.0%
	CCTATTACTTTGGACACTCC+GGG	+	Chr5:55219747-55219766	MsG0580027169.01.T01:five_prime_UTR	45.0%
	CGAACAGATGAAGCAGAATC+AGG	-	Chr5:55223928-55223947	None:intergenic	45.0%
	CGCGAAAACTCAAGGTCTTT+AGG	+	Chr5:55221021-55221040	MsG0580027169.01.T01:intron	45.0%
	GAGTGAGGAGAACAGAAAGA+TGG	+	Chr5:55220877-55220896	MsG0580027169.01.T01:intron	45.0%
	GATGCTGCCAAAGCTTCATA+TGG	-	Chr5:55222807-55222826	None:intergenic	45.0%
	GCAGGAGTCCAACATCTTAT+TGG	-	Chr5:55220504-55220523	None:intergenic	45.0%
	GGATGCTATTGCTTCTGCTA+AGG	+	Chr5:55221308-55221327	MsG0580027169.01.T01:CDS	45.0%
	GGCCATCTCTTGGTATAACA+GGG	+	Chr5:55222875-55222894	MsG0580027169.01.T01:CDS	45.0%
	GTATTCAAGAGGTTCGAACC+AGG	+	Chr5:55221355-55221374	MsG0580027169.01.T01:CDS	45.0%
	GTTTACTACATCTCTGGTGC+CGG	+	Chr5:55222173-55222192	MsG0580027169.01.T01:intron	45.0%
	TAACATGCGTAGGGGCAATT+TGG	+	Chr5:55220397-55220416	MsG0580027169.01.T01:intron	45.0%
	TCTCAACCATGTGACTTCTC+GGG	+	Chr5:55222219-55222238	MsG0580027169.01.T01:intron	45.0%
	TCTGAAGGGACGTTGAATGA+GGG	+	Chr5:55222825-55222844	MsG0580027169.01.T01:CDS	45.0%
	TGATGTCGACAACCATGGAT+TGG	-	Chr5:55221737-55221756	None:intergenic	45.0%
	TGGCCATCTCTTGGTATAAC+AGG	+	Chr5:55222874-55222893	MsG0580027169.01.T01:CDS	45.0%
	TTTACTACATCTCTGGTGCC+GGG	+	Chr5:55222174-55222193	MsG0580027169.01.T01:intron	45.0%
!	AAGCTTTGGCAGCATCTGAA+GGG	+	Chr5:55222811-55222830	MsG0580027169.01.T01:intron	45.0%
!	CTTCGATGGTATTGAAGAGC+TGG	+	Chr5:55223211-55223230	MsG0580027169.01.T01:CDS	45.0%
!	GAATTGACTTAGTGCCCGTT+TGG	+	Chr5:55221514-55221533	MsG0580027169.01.T01:intron	45.0%
!	GTGTGATTTCTGATTCGCAG+TGG	-	Chr5:55220922-55220941	None:intergenic	45.0%
!	GTTGGTTTCTTTGACGGCTT+CGG	-	Chr5:55219714-55219733	None:intergenic	45.0%
!	TGGTTAAAGTACTCGCAGGT+TGG	+	Chr5:55221165-55221184	MsG0580027169.01.T01:intron	45.0%
!!	ATCTCTTGCTTTGGCTTTCC+CGG	-	Chr5:55219768-55219787	None:intergenic	45.0%
!!	CTCGCAGGTTGGTTTGTATT+AGG	+	Chr5:55221176-55221195	MsG0580027169.01.T01:intron	45.0%
!!	TATGACACTTTTGGGTCGGT+CGG	+	Chr5:55219844-55219863	MsG0580027169.01.T01:intron	45.0%
!!!	AGTTTTCGCGGTTTGTTGTG+CGG	-	Chr5:55221011-55221030	None:intergenic	45.0%
!!!	GGTGGTTTTGATGGATTGGA+AGG	-	Chr5:55220990-55221009	None:intergenic	45.0%
!!!	TCGGTGTTCGTCCTTTTTTG+TGG	+	Chr5:55219863-55219882	MsG0580027169.01.T01:intron	45.0%
!!!	TTGTTGTGCGGTGGTTTTGA+TGG	-	Chr5:55220999-55221018	None:intergenic	45.0%
	AAACACAGGTGGCCATCTCT+TGG	+	Chr5:55222865-55222884	MsG0580027169.01.T01:CDS	50.0%
	AAGCTCCAACGTTGTTGTGG+TGG	-	Chr5:55220820-55220839	None:intergenic	50.0%
	AGCTCCAACGTTGTTGTGGT+GGG	-	Chr5:55220819-55220838	None:intergenic	50.0%
	CTCTTCAATGGTTCGAGACC+CGG	-	Chr5:55222195-55222214	None:intergenic	50.0%
	GACTTAGTGCCCGTTTGGAT+TGG	+	Chr5:55221519-55221538	MsG0580027169.01.T01:intron	50.0%
	GATGTGGGAGCTCTGCATAT+TGG	+	Chr5:55223358-55223377	MsG0580027169.01.T01:intron	50.0%
	GCTTCTGCTAAGGCTAGAGT+TGG	+	Chr5:55221318-55221337	MsG0580027169.01.T01:CDS	50.0%
	TCACTTCTACCAGATGTGCC+AGG	-	Chr5:55223802-55223821	None:intergenic	50.0%
	TTTCATGTCAAAGGGGGACG+TGG	+	Chr5:55222906-55222925	MsG0580027169.01.T01:CDS	50.0%
!	CCCGGAGTGTCCAAAGTAAT+AGG	-	Chr5:55219750-55219769	None:intergenic	50.0%
!	GAAGCTTTGGCAGCATCTGA+AGG	+	Chr5:55222810-55222829	MsG0580027169.01.T01:intron	50.0%
!	GGGTTTTGCTGGGTGGTTAT+TGG	+	Chr5:55222151-55222170	MsG0580027169.01.T01:intron	50.0%
!!	TTCTGATTCGCAGTGGGTTG+AGG	-	Chr5:55220915-55220934	None:intergenic	50.0%
!!!	GGTCGTTTTGGGGTTTTGCT+GGG	+	Chr5:55222141-55222160	MsG0580027169.01.T01:intron	50.0%
!!!	GTGCGGTGGTTTTGATGGAT+TGG	-	Chr5:55220994-55221013	None:intergenic	50.0%
!!!	TGGTCGTTTTGGGGTTTTGC+TGG	+	Chr5:55222140-55222159	MsG0580027169.01.T01:intron	50.0%
	AAGTGGAACGCTCTGGCAGT+AGG	+	Chr5:55223848-55223867	MsG0580027169.01.T01:CDS	55.0%
	ACTTCTCGGGAGACAGGCAT+TGG	+	Chr5:55222232-55222251	MsG0580027169.01.T01:intron	55.0%
	AGTGGAACGCTCTGGCAGTA+GGG	+	Chr5:55223849-55223868	MsG0580027169.01.T01:CDS	55.0%
	CATGTGACTTCTCGGGAGAC+AGG	+	Chr5:55222226-55222245	MsG0580027169.01.T01:intron	55.0%
	CTGTCTCCCGAGAAGTCACA+TGG	-	Chr5:55222228-55222247	None:intergenic	55.0%
	GAGAAGATGTGGGAGGTGAG+AGG	-	Chr5:55222777-55222796	None:intergenic	55.0%
	GAGCATGAAGTGGAACGCTC+TGG	+	Chr5:55223841-55223860	MsG0580027169.01.T01:CDS	55.0%
	GCGAAAAGAGGCTAGGCGAA+TGG	+	Chr5:55221278-55221297	MsG0580027169.01.T01:exon	55.0%
	TCGCCAGTCTCCACGTTTGA+AGG	+	Chr5:55223945-55223964	MsG0580027169.01.T01:CDS	55.0%
	TCTACCAGATGTGCCAGGAG+CGG	-	Chr5:55223797-55223816	None:intergenic	55.0%
!!	TGATTCGCAGTGGGTTGAGG+AGG	-	Chr5:55220912-55220931	None:intergenic	55.0%
!!	TTTCGCGGTTTGTTGTGCGG+TGG	-	Chr5:55221008-55221027	None:intergenic	55.0%
!!!	CGTTTTGGGGTTTTGCTGGG+TGG	+	Chr5:55222144-55222163	MsG0580027169.01.T01:intron	55.0%
	CTGGCCCACCACAACAACGT+TGG	+	Chr5:55220812-55220831	MsG0580027169.01.T01:intron	60.0%
	TCATCCGCTCCTGGCACATC+TGG	+	Chr5:55223790-55223809	MsG0580027169.01.T01:CDS	60.0%
!	GGCTAGGCGAATGGCTCTGT+TGG	+	Chr5:55221287-55221306	MsG0580027169.01.T01:exon	60.0%
!!	GGAAGCGGCGAAAGCGTCGA+CGG	-	Chr5:55220957-55220976	None:intergenic	65.0%
!!	CGGCGAAAGCGTCGACGGTG+TGG	-	Chr5:55220952-55220971	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	55219699	55223972	55219699	ID=MsG0580027169.01;Name=MsG0580027169.01
Chr5	mRNA	55219699	55223972	55219699	ID=MsG0580027169.01.T01;Parent=MsG0580027169.01;Name=MsG0580027169.01.T01;_AED=0.47;_eAED=0.49;_QI=402\|0.71\|0.5\|0.87\|0.71\|0.75\|8\|0\|223
Chr5	exon	55219699	55219832	55219699	ID=MsG0580027169.01.T01:exon:41478;Parent=MsG0580027169.01.T01
Chr5	exon	55220887	55221034	55220887	ID=MsG0580027169.01.T01:exon:41479;Parent=MsG0580027169.01.T01
Chr5	exon	55221089	55221182	55221089	ID=MsG0580027169.01.T01:exon:41480;Parent=MsG0580027169.01.T01
Chr5	exon	55221271	55221417	55221271	ID=MsG0580027169.01.T01:exon:41481;Parent=MsG0580027169.01.T01
Chr5	exon	55222823	55222927	55222823	ID=MsG0580027169.01.T01:exon:41482;Parent=MsG0580027169.01.T01
Chr5	exon	55223016	55223105	55223016	ID=MsG0580027169.01.T01:exon:41483;Parent=MsG0580027169.01.T01
Chr5	exon	55223192	55223314	55223192	ID=MsG0580027169.01.T01:exon:41484;Parent=MsG0580027169.01.T01
Chr5	exon	55223740	55223972	55223740	ID=MsG0580027169.01.T01:exon:41485;Parent=MsG0580027169.01.T01
Chr5	five_prime_UTR	55219699	55219832	55219699	ID=MsG0580027169.01.T01:five_prime_utr;Parent=MsG0580027169.01.T01
Chr5	five_prime_UTR	55220887	55221034	55220887	ID=MsG0580027169.01.T01:five_prime_utr;Parent=MsG0580027169.01.T01
Chr5	five_prime_UTR	55221089	55221182	55221089	ID=MsG0580027169.01.T01:five_prime_utr;Parent=MsG0580027169.01.T01
Chr5	five_prime_UTR	55221271	55221296	55221271	ID=MsG0580027169.01.T01:five_prime_utr;Parent=MsG0580027169.01.T01
Chr5	CDS	55221297	55221417	55221297	ID=MsG0580027169.01.T01:cds;Parent=MsG0580027169.01.T01
Chr5	CDS	55222823	55222927	55222823	ID=MsG0580027169.01.T01:cds;Parent=MsG0580027169.01.T01
Chr5	CDS	55223016	55223105	55223016	ID=MsG0580027169.01.T01:cds;Parent=MsG0580027169.01.T01
Chr5	CDS	55223192	55223314	55223192	ID=MsG0580027169.01.T01:cds;Parent=MsG0580027169.01.T01
Chr5	CDS	55223740	55223972	55223740	ID=MsG0580027169.01.T01:cds;Parent=MsG0580027169.01.T01

Gene Sequence

>MsG0580027169.01.T01

ATGGCTCTGTTGGATGCTATTGCTTCTGCTAAGGCTAGAGTTGGAAATTTCAACTCAAGTATTCAAGAGGTTCGAACCAGGAAACAAGAATATAACTCGTTTCTATCGCAGCAATCACTTGCATCTGAAGGGACGTTGAATGAGGGCATTGAACATACAGATGAAACACAGGTGGCCATCTCTTGGTATAACAGGGTACTTGGTTTTCATGTCAAAGGGGGACGTGGGGTAAAATTCACGTTCAAGAATATAAATTTGAAGAATCCAAATGAGGAGTACTCTTTTACTATCTTCCATGATAAAAATACTTATACATTGTTACATTGTGAACCTTCCTTCGATGGTATTGAAGAGCTGGTCCATGAATTAAACAAGACAGATGGTCTGTTTAAGTTTGTCAGAGTAATGAGGAAAAAATTTCAAGAAGCATTGGCACAAGGGAGCTTAGTTCTAACAACAGTTAATCCTGGAGAATCTGCCTTTATATCTTCATCCGCTCCTGGCACATCTGGTAGAAGTGATTCTACAGCCATGGAAAATGAGCATGAAGTGGAACGCTCTGGCAGTAGGGCACAATTAAAGAAAAAACAAGTTCGCAGAAGGAAAAGTTTGGCACTTTTATCTCCTGATTCTGCTTCATCTGTTCGCCAGTCTCCACGTTTGAAGGTATGA

Protein sequence

>MsG0580027169.01.T01

MALLDAIASAKARVGNFNSSIQEVRTRKQEYNSFLSQQSLASEGTLNEGIEHTDETQVAISWYNRVLGFHVKGGRGVKFTFKNINLKNPNEEYSFTIFHDKNTYTLLHCEPSFDGIEELVHELNKTDGLFKFVRVMRKKFQEALAQGSLVLTTVNPGESAFISSSAPGTSGRSDSTAMENEHEVERSGSRAQLKKKQVRRRKSLALLSPDSASSVRQSPRLKV*