AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040574.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040574.01.T01 MTR_7g093030 61.207 232 12 4 16 169 89 320 2.03e-67 211
MsG0780040574.01.T01 MTR_7g093030 60.000 200 6 3 16 141 89 288 8.92e-55 176
MsG0780040574.01.T01 MTR_1g053835 41.667 228 58 6 17 169 138 365 1.57e-39 139
MsG0780040574.01.T01 MTR_1g053830 57.143 49 21 0 87 135 1 49 2.50e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040574.01.T01 AT5G06800 40.667 150 41 6 66 169 153 300 3.31e-19 84.0
MsG0780040574.01.T01 AT5G06800 39.355 155 41 6 66 169 153 305 1.43e-18 82.4
MsG0780040574.01.T01 AT5G06800 49.451 91 41 4 66 152 153 242 2.08e-14 69.7

Find 32 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATATAAGGTTAATGATT+TGG 0.225266 7:-79380686 None:intergenic
TAAGGGAAAACAATCATTAT+TGG 0.254875 7:-79380643 MsG0780040574.01.T01:CDS
TTCCCTTGTGTCAATCTCTA+AGG 0.258329 7:-79380661 MsG0780040574.01.T01:CDS
ACAGCTGCACATCATCTATA+TGG 0.357735 7:+79379250 None:intergenic
ATCATTAAAATCAGTTTGTA+AGG 0.371660 7:-79378925 MsG0780040574.01.T01:intron
AAACTTCCTGCTGAAATTGT+TGG 0.389210 7:+79379993 None:intergenic
TTGAGTGTGTGAATCGCCTT+GGG 0.396796 7:-79379878 MsG0780040574.01.T01:CDS
GGAAGTTTATCAACTTCTCT+TGG 0.397953 7:-79379978 MsG0780040574.01.T01:CDS
GTGTTAGACAGTTCAAAGTT+GGG 0.442345 7:+79380600 None:intergenic
GTCTAACACTTTGCAAGCAT+TGG 0.457506 7:-79380586 MsG0780040574.01.T01:CDS
GTTGAGTGTGTGAATCGCCT+TGG 0.458117 7:-79379879 MsG0780040574.01.T01:CDS
AGTGTTAGACAGTTCAAAGT+TGG 0.458507 7:+79380599 None:intergenic
CAAGTAAAACAAGAATTAGA+TGG 0.463217 7:-79379926 MsG0780040574.01.T01:CDS
TTTGCAGTGGCTTCAAATCA+AGG 0.481491 7:-79379080 MsG0780040574.01.T01:intron
AACAAGAATTAGATGGACTA+AGG 0.489147 7:-79379919 MsG0780040574.01.T01:CDS
TTGGTGAAATCTCAATTGAA+TGG 0.505462 7:-79380567 MsG0780040574.01.T01:CDS
CATCCGTTTGCAGGTGCTCG+TGG 0.509077 7:-79380072 MsG0780040574.01.T01:intron
GTTCAAAGTTGGGGTCATGC+TGG 0.509873 7:+79380610 None:intergenic
CTTGGAAGCACTTCTTCTAG+TGG 0.515481 7:-79379960 MsG0780040574.01.T01:CDS
TTGTCTCCAACAATTTCAGC+AGG 0.521373 7:-79379999 MsG0780040574.01.T01:CDS
AACATTACAATTGCGACTTG+AGG 0.551481 7:-79378790 MsG0780040574.01.T01:CDS
TTGGAAGCACTTCTTCTAGT+GGG 0.558294 7:-79379959 MsG0780040574.01.T01:CDS
TCCCTTGTGTCAATCTCTAA+GGG 0.561596 7:-79380660 MsG0780040574.01.T01:CDS
TGTTAGACAGTTCAAAGTTG+GGG 0.605516 7:+79380601 None:intergenic
TCCCTTAGAGATTGACACAA+GGG 0.605574 7:+79380659 None:intergenic
GCGTCTTCATGAACAACTAG+AGG 0.618964 7:-79379026 MsG0780040574.01.T01:intron
CAATTGCGACTTGAGGAACA+AGG 0.643962 7:-79378783 MsG0780040574.01.T01:CDS
TATAGATGATGTGCAGCTGT+TGG 0.648976 7:-79379247 MsG0780040574.01.T01:intron
TTCCCTTAGAGATTGACACA+AGG 0.667084 7:+79380658 None:intergenic
AACTTCAATTAGATGCACAA+AGG 0.668848 7:-79379048 MsG0780040574.01.T01:CDS
GAGTGTGTGAATCGCCTTGG+GGG 0.715113 7:-79379876 MsG0780040574.01.T01:CDS
TGAGTGTGTGAATCGCCTTG+GGG 0.716876 7:-79379877 MsG0780040574.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACCTTATTTTTATTTTATAT+GGG - Chr7:79380574-79380593 MsG0780040574.01.T01:CDS 10.0%
!! TCCCATATAAAATAAAAATA+AGG + Chr7:79380578-79380597 None:intergenic 15.0%
!!! GACCTTATTTTTATTTTATA+TGG - Chr7:79380573-79380592 MsG0780040574.01.T01:CDS 15.0%
!!! TAAGAGTATAAAAGTATTTT+AGG + Chr7:79380252-79380271 None:intergenic 15.0%
!! AAACTTGAATAAATTTCATG+TGG - Chr7:79378924-79378943 MsG0780040574.01.T01:intron 20.0%
!! AACTTGAATAAATTTCATGT+GGG - Chr7:79378925-79378944 MsG0780040574.01.T01:intron 20.0%
!! AAGTTCATAAAGAAAATGAA+TGG + Chr7:79380454-79380473 None:intergenic 20.0%
!! AGTATAAATATGTTATGACA+CGG + Chr7:79379185-79379204 None:intergenic 20.0%
!! ATCATTAAAATCAGTTTGTA+AGG - Chr7:79380519-79380538 MsG0780040574.01.T01:intron 20.0%
!! ATGAAATTAATCAATCAACT+TGG + Chr7:79380289-79380308 None:intergenic 20.0%
!!! AACTGATTTTAATGATGATT+AGG + Chr7:79380515-79380534 None:intergenic 20.0%
!!! ACATTAGTTTGTTCAATATT+AGG + Chr7:79380143-79380162 None:intergenic 20.0%
!!! GAAATTTATTCAAGTTTTCA+TGG + Chr7:79378921-79378940 None:intergenic 20.0%
! AAAAGCTATATTGAAGATGA+TGG - Chr7:79379691-79379710 MsG0780040574.01.T01:intron 25.0%
! AAATTAATCAATCAACTTGG+TGG + Chr7:79380286-79380305 None:intergenic 25.0%
! ACATTATTCACTATGAACAA+TGG + Chr7:79379970-79379989 None:intergenic 25.0%
! ATAAATCATCGTATCACATT+AGG - Chr7:79379317-79379336 MsG0780040574.01.T01:intron 25.0%
! CAAGTAAAACAAGAATTAGA+TGG - Chr7:79379518-79379537 MsG0780040574.01.T01:intron 25.0%
! CATTATTCACTATGAACAAT+GGG + Chr7:79379969-79379988 None:intergenic 25.0%
! CTCAACATGAAAAATAAACT+AGG + Chr7:79379848-79379867 None:intergenic 25.0%
! GAAAGAAAATAAGCACATTT+GGG + Chr7:79380099-79380118 None:intergenic 25.0%
! TAAAATCAGTTTGTAAGGTA+AGG - Chr7:79380524-79380543 MsG0780040574.01.T01:CDS 25.0%
! TAAGGGAAAACAATCATTAT+TGG - Chr7:79378801-79378820 MsG0780040574.01.T01:CDS 25.0%
! TAATGGAAATCATGACTATA+TGG - Chr7:79379040-79379059 MsG0780040574.01.T01:CDS 25.0%
! TGAAAGAAAATAAGCACATT+TGG + Chr7:79380100-79380119 None:intergenic 25.0%
! TGAATAATGTCTGGTTAAAT+AGG - Chr7:79379980-79379999 MsG0780040574.01.T01:CDS 25.0%
! TGTATTTATATCCACTAGAA+TGG - Chr7:79380036-79380055 MsG0780040574.01.T01:CDS 25.0%
!! AATGACTTTTCACATGAATA+TGG + Chr7:79379732-79379751 None:intergenic 25.0%
!! AGCTCATCATTTTGTAATAA+TGG - Chr7:79379023-79379042 MsG0780040574.01.T01:intron 25.0%
!! ATCATCTTCAATATAGCTTT+TGG + Chr7:79379692-79379711 None:intergenic 25.0%
!! TATCTGATAGTCTTTTCTAT+TGG - Chr7:79379644-79379663 MsG0780040574.01.T01:intron 25.0%
!! TTCTAATCATGTTGTTACTT+TGG - Chr7:79380618-79380637 MsG0780040574.01.T01:CDS 25.0%
!!! AATTTGTTATAGGTTGTCAA+TGG - Chr7:79379920-79379939 MsG0780040574.01.T01:CDS 25.0%
!!! GTGATAAGCAAATTTGTTAT+AGG - Chr7:79379910-79379929 MsG0780040574.01.T01:CDS 25.0%
AAACTAATGTGTCCAATTTC+AGG - Chr7:79380153-79380172 MsG0780040574.01.T01:intron 30.0%
AACTAATGTGTCCAATTTCA+GGG - Chr7:79380154-79380173 MsG0780040574.01.T01:intron 30.0%
AACTTCAATTAGATGCACAA+AGG - Chr7:79380396-79380415 MsG0780040574.01.T01:intron 30.0%
CATAAAGAAAATGAATGGCA+AGG + Chr7:79380449-79380468 None:intergenic 30.0%
TGTTCATAGTGAATAATGTC+TGG - Chr7:79379971-79379990 MsG0780040574.01.T01:CDS 30.0%
TTGGTGAAATCTCAATTGAA+TGG - Chr7:79378877-79378896 MsG0780040574.01.T01:intron 30.0%
TTTGCAAAATGATTCTTCTG+CGG - Chr7:79379071-79379090 MsG0780040574.01.T01:CDS 30.0%
! TCAAGTTTTCATGGAATCTA+AGG + Chr7:79378912-79378931 None:intergenic 30.0%
!! AACAAGAATTAGATGGACTA+AGG - Chr7:79379525-79379544 MsG0780040574.01.T01:intron 30.0%
!! TATTGGTTTCTTGCTTATAG+AGG - Chr7:79379661-79379680 MsG0780040574.01.T01:intron 30.0%
!!! AACAACTTTTGCTGTTCAAT+TGG + Chr7:79378975-79378994 None:intergenic 30.0%
!!! TTTTGCTGTTCAATTGGAAA+AGG + Chr7:79378969-79378988 None:intergenic 30.0%
AAACTTCCTGCTGAAATTGT+TGG + Chr7:79379454-79379473 None:intergenic 35.0%
AACATTACAATTGCGACTTG+AGG - Chr7:79380654-79380673 MsG0780040574.01.T01:CDS 35.0%
AGTGTTAGACAGTTCAAAGT+TGG + Chr7:79378848-79378867 None:intergenic 35.0%
CAGCAAAAGTTGTTCTTTGA+TGG - Chr7:79378981-79379000 MsG0780040574.01.T01:intron 35.0%
GGAAGTTTATCAACTTCTCT+TGG - Chr7:79379466-79379485 MsG0780040574.01.T01:intron 35.0%
GTGTTAGACAGTTCAAAGTT+GGG + Chr7:79378847-79378866 None:intergenic 35.0%
GTTATAGGTTGTCAATGGAT+AGG - Chr7:79379925-79379944 MsG0780040574.01.T01:CDS 35.0%
GTTGTCAATGGATAGGATAT+AGG - Chr7:79379932-79379951 MsG0780040574.01.T01:CDS 35.0%
TAATTATAGCATCCGTTTGC+AGG - Chr7:79379363-79379382 MsG0780040574.01.T01:intron 35.0%
TGTTAGACAGTTCAAAGTTG+GGG + Chr7:79378846-79378865 None:intergenic 35.0%
TTGTCAGATTTCCCTGAAAT+TGG + Chr7:79380168-79380187 None:intergenic 35.0%
! ATTTCATGTGGGAATTTTCC+TGG - Chr7:79378936-79378955 MsG0780040574.01.T01:intron 35.0%
! TTTCATGTGGGAATTTTCCT+GGG - Chr7:79378937-79378956 MsG0780040574.01.T01:intron 35.0%
AACGATCAAGATACTACTGG+AGG - Chr7:79379137-79379156 MsG0780040574.01.T01:intron 40.0%
ACAGCTGCACATCATCTATA+TGG + Chr7:79380197-79380216 None:intergenic 40.0%
CAACACTTCTACCATTCTAG+TGG + Chr7:79380050-79380069 None:intergenic 40.0%
GTCTAACACTTTGCAAGCAT+TGG - Chr7:79378858-79378877 MsG0780040574.01.T01:intron 40.0%
TATAGATGATGTGCAGCTGT+TGG - Chr7:79380197-79380216 MsG0780040574.01.T01:intron 40.0%
TCCAACGATCAAGATACTAC+TGG - Chr7:79379134-79379153 MsG0780040574.01.T01:intron 40.0%
TCCAGTAGTATCTTGATCGT+TGG + Chr7:79379138-79379157 None:intergenic 40.0%
TCCCTTAGAGATTGACACAA+GGG + Chr7:79378788-79378807 None:intergenic 40.0%
TCCCTTGTGTCAATCTCTAA+GGG - Chr7:79378784-79378803 MsG0780040574.01.T01:CDS 40.0%
TTCCCTTAGAGATTGACACA+AGG + Chr7:79378789-79378808 None:intergenic 40.0%
TTCCCTTGTGTCAATCTCTA+AGG - Chr7:79378783-79378802 MsG0780040574.01.T01:CDS 40.0%
TTGAGAATCCTTGCATCTCA+CGG - Chr7:79379208-79379227 MsG0780040574.01.T01:intron 40.0%
TTGTCTCCAACAATTTCAGC+AGG - Chr7:79379445-79379464 MsG0780040574.01.T01:intron 40.0%
TTTGCAGTGGCTTCAAATCA+AGG - Chr7:79380364-79380383 MsG0780040574.01.T01:intron 40.0%
!! TTGAAGATGATGGACTCTGA+AGG - Chr7:79379701-79379720 MsG0780040574.01.T01:intron 40.0%
!! TTGGAAGCACTTCTTCTAGT+GGG - Chr7:79379485-79379504 MsG0780040574.01.T01:intron 40.0%
AAGCCACTGCAAATCAAGAG+TGG + Chr7:79380357-79380376 None:intergenic 45.0%
CAATTGCGACTTGAGGAACA+AGG - Chr7:79380661-79380680 MsG0780040574.01.T01:CDS 45.0%
GATCAAGATACTACTGGAGG+TGG - Chr7:79379140-79379159 MsG0780040574.01.T01:intron 45.0%
GCGTCTTCATGAACAACTAG+AGG - Chr7:79380418-79380437 MsG0780040574.01.T01:intron 45.0%
GTTATGACACGGATCCTCTT+CGG + Chr7:79379174-79379193 None:intergenic 45.0%
TGAGAATCCTTGCATCTCAC+GGG - Chr7:79379209-79379228 MsG0780040574.01.T01:intron 45.0%
TGATGAGCTGTTGCTAACTG+AGG + Chr7:79379012-79379031 None:intergenic 45.0%
TTGAGTGTGTGAATCGCCTT+GGG - Chr7:79379566-79379585 MsG0780040574.01.T01:intron 45.0%
! GAATTACCTTGAGCTGACTC+TGG + Chr7:79379784-79379803 None:intergenic 45.0%
!! ACACCACTCTTGATTTGCAG+TGG - Chr7:79380351-79380370 MsG0780040574.01.T01:intron 45.0%
!! CTTGGAAGCACTTCTTCTAG+TGG - Chr7:79379484-79379503 MsG0780040574.01.T01:intron 45.0%
AATCCACGAGCACCTGCAAA+CGG + Chr7:79379378-79379397 None:intergenic 50.0%
ATTGAACGACCAAGCTCTGC+TGG + Chr7:79379111-79379130 None:intergenic 50.0%
CAAGATACTACTGGAGGTGG+TGG - Chr7:79379143-79379162 MsG0780040574.01.T01:intron 50.0%
GAGAATCCTTGCATCTCACG+GGG - Chr7:79379210-79379229 MsG0780040574.01.T01:intron 50.0%
GTTCAAAGTTGGGGTCATGC+TGG + Chr7:79378837-79378856 None:intergenic 50.0%
GTTGAGTGTGTGAATCGCCT+TGG - Chr7:79379565-79379584 MsG0780040574.01.T01:intron 50.0%
TGAGTGTGTGAATCGCCTTG+GGG - Chr7:79379567-79379586 MsG0780040574.01.T01:intron 50.0%
TTCATGCCAGAGTCAGCTCA+AGG - Chr7:79379775-79379794 MsG0780040574.01.T01:intron 50.0%
TTTCTTCCCCGTGAGATGCA+AGG + Chr7:79379219-79379238 None:intergenic 50.0%
! ATTGGAAAAGGCTTGCTCCC+AGG + Chr7:79378957-79378976 None:intergenic 50.0%
ACTTACTCTCAGCACCCCCA+AGG + Chr7:79379585-79379604 None:intergenic 55.0%
CTCTGCTGGAGAGGTAGAAG+AGG + Chr7:79379097-79379116 None:intergenic 55.0%
GAGTGTGTGAATCGCCTTGG+GGG - Chr7:79379568-79379587 MsG0780040574.01.T01:intron 55.0%
TCTACCTCTCCAGCAGAGCT+TGG - Chr7:79379099-79379118 MsG0780040574.01.T01:intron 55.0%
ACGACCAAGCTCTGCTGGAG+AGG + Chr7:79379106-79379125 None:intergenic 60.0%
CATCCGTTTGCAGGTGCTCG+TGG - Chr7:79379372-79379391 MsG0780040574.01.T01:intron 60.0%
!! AGGTGGTGGAGAGACCGAAG+AGG - Chr7:79379157-79379176 MsG0780040574.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79378772 79380694 79378772 ID=MsG0780040574.01;Name=MsG0780040574.01
Chr7 mRNA 79378772 79380694 79378772 ID=MsG0780040574.01.T01;Parent=MsG0780040574.01;Name=MsG0780040574.01.T01;_AED=0.50;_eAED=0.55;_QI=0|0|0|1|1|1|6|0|184
Chr7 exon 79380524 79380694 79380524 ID=MsG0780040574.01.T01:exon:16287;Parent=MsG0780040574.01.T01
Chr7 exon 79379868 79380081 79379868 ID=MsG0780040574.01.T01:exon:16286;Parent=MsG0780040574.01.T01
Chr7 exon 79379248 79379291 79379248 ID=MsG0780040574.01.T01:exon:16285;Parent=MsG0780040574.01.T01
Chr7 exon 79379027 79379095 79379027 ID=MsG0780040574.01.T01:exon:16284;Parent=MsG0780040574.01.T01
Chr7 exon 79378926 79378933 79378926 ID=MsG0780040574.01.T01:exon:16283;Parent=MsG0780040574.01.T01
Chr7 exon 79378772 79378820 79378772 ID=MsG0780040574.01.T01:exon:16282;Parent=MsG0780040574.01.T01
Chr7 CDS 79380524 79380694 79380524 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Chr7 CDS 79379868 79380081 79379868 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Chr7 CDS 79379248 79379291 79379248 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Chr7 CDS 79379027 79379095 79379027 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Chr7 CDS 79378926 79378933 79378926 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Chr7 CDS 79378772 79378820 79378772 ID=MsG0780040574.01.T01:cds;Parent=MsG0780040574.01.T01
Gene Sequence

>MsG0780040574.01.T01

ATGATTTGGAATTCCCTTGTGTCAATCTCTAAGGGAAAACAATCATTATTGGATGCATCAAACCAGCATGACCCCAACTTTGAACTGTCTAACACTTTGCAAGCATTGGTGAAATCTCAATTGAATGGTAATCAACACCTTAGATTCCATGAAAACTTGAATAAATTTCATGTGCTCGTGGATTATCATTTATCTGTTGAGCAACTGAACTTCTCTTCTCAGCATGAGAAGTTGTCTCCAACAATTTCAGCAGGAAGTTTATCAACTTCTCTTGGAAGCACTTCTTCTAGTGGGAATGTAGTCTCAAGTAAAACAAGAATTAGATGGACTAAGGATCTTCATGAGAAGTTTGTTGAGTGTGTGAATCGCCTTGGGGGTGCTGAGAGGAAATCTGACAAAAGAATCCATATAGATGATGTGCAGCTGTTGTGGCTTCAAATCAAGGAAGCACTTCAACTTCAATTAGATGCACAAAGGCGTCTTCATGAACAACTAGAGTTTGTAAGATTCAAAGAACATTACAATTGCGACTTGAGGAACAAGGCAGACAGCTGA

Protein sequence

>MsG0780040574.01.T01

MIWNSLVSISKGKQSLLDASNQHDPNFELSNTLQALVKSQLNGNQHLRFHENLNKFHVLVDYHLSVEQLNFSSQHEKLSPTISAGSLSTSLGSTSSSGNVVSSKTRIRWTKDLHEKFVECVNRLGGAERKSDKRIHIDDVQLLWLQIKEALQLQLDAQRRLHEQLEFVRFKEHYNCDLRNKADS*