AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046219.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046219.01.T01 MTR_4g103330 95.187 187 9 0 1 187 62 248 1.47e-119 338
MsG0880046219.01.T01 MTR_7g117900 29.197 137 89 3 6 141 82 211 1.64e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046219.01.T01 AT4G09650 63.102 187 69 0 1 187 47 233 2.77e-84 249

Find 46 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCGTAGCTGGCGGCGGC+TGG 0.162799 8:-70686958 None:intergenic
ACCGTCAGGTATGGGAATTC+TGG 0.172359 8:+70687405 MsG0880046219.01.T01:CDS
TTGGTGGAGTCTTCGATAAT+TGG 0.238527 8:-70687093 None:intergenic
AATTGCTGCTCAACTTGATT+TGG 0.271511 8:+70687470 MsG0880046219.01.T01:CDS
TGGCGTAGCTGGCGGCGGCT+GGG 0.280536 8:-70686957 None:intergenic
AACTGCTCTTGATCCAAGTT+TGG 0.310216 8:+70687371 MsG0880046219.01.T01:CDS
GAAATTGGAGTCACAGCATT+TGG 0.323866 8:+70687293 MsG0880046219.01.T01:CDS
TTTGGCGCAGATTGCGAAAC+AGG 0.327217 8:+70687311 MsG0880046219.01.T01:CDS
GTCGAGGGTGTTGTTGGATT+TGG 0.360334 8:-70686998 None:intergenic
GAGAAATAGTCGAACACCTT+GGG 0.363815 8:-70687066 None:intergenic
ATTGCTGCTCAACTTGATTT+GGG 0.372073 8:+70687471 MsG0880046219.01.T01:CDS
CCGTCAGGTATGGGAATTCT+GGG 0.374285 8:+70687406 MsG0880046219.01.T01:CDS
GGTGTCTTCAGTGGTGAAAT+TGG 0.383089 8:+70687278 MsG0880046219.01.T01:CDS
GGCTAATGCGGTGGCGTAGC+TGG 0.387942 8:-70686968 None:intergenic
AGCGATGAGTTTGCGTTTGG+TGG 0.395419 8:-70687109 None:intergenic
GTAAAACCCGCCACCAAACT+TGG 0.397977 8:-70687384 None:intergenic
TGGTGGAGTCTTCGATAATT+GGG 0.398456 8:-70687092 None:intergenic
GGCGTAGCTGGCGGCGGCTG+GGG 0.401952 8:-70686956 None:intergenic
GTTGTTGGATTTGGCTAAAT+CGG 0.409703 8:-70686989 None:intergenic
TTCAGCGATGAGTTTGCGTT+TGG 0.420804 8:-70687112 None:intergenic
GGTGGCGTCGAGGGTGTTGT+TGG 0.434585 8:-70687004 None:intergenic
TGCGGTGGCGTAGCTGGCGG+CGG 0.438767 8:-70686962 None:intergenic
TTTGTCGATGTCAGCGGTGG+TGG 0.445558 8:-70687022 None:intergenic
GTCAGCGGTGGTGGCGTCGA+GGG 0.465818 8:-70687013 None:intergenic
CTTGATCCAAGTTTGGTGGC+GGG 0.474377 8:+70687378 MsG0880046219.01.T01:CDS
TGCTCTTGATCCAAGTTTGG+TGG 0.485731 8:+70687374 MsG0880046219.01.T01:CDS
TCTTGATCCAAGTTTGGTGG+CGG 0.512469 8:+70687377 MsG0880046219.01.T01:CDS
TTCTGTTTGCTTCGATGAGG+AGG 0.515130 8:-70687182 None:intergenic
TTGCTTCGATGAGGAGGTTG+AGG 0.520100 8:-70687176 None:intergenic
GGAGTCTTCGATAATTGGGC+TGG 0.533217 8:-70687088 None:intergenic
GGAGAAATAGTCGAACACCT+TGG 0.545934 8:-70687067 None:intergenic
CGATTCTGTTTGCTTCGATG+AGG 0.552223 8:-70687185 None:intergenic
TAATGCGGTGGCGTAGCTGG+CGG 0.559050 8:-70686965 None:intergenic
GGCTAAATCGGCTAATGCGG+TGG 0.571556 8:-70686977 None:intergenic
GCTTGTTGTGGTGTCTTCAG+TGG 0.590381 8:+70687269 MsG0880046219.01.T01:CDS
TGTCAGCGGTGGTGGCGTCG+AGG 0.591340 8:-70687014 None:intergenic
AGAACTCTTCACTGACCCCA+AGG 0.615212 8:+70687050 MsG0880046219.01.T01:CDS
CGAATTCTCTCACGATGTCG+AGG 0.616657 8:-70687215 None:intergenic
TTTGGCTAAATCGGCTAATG+CGG 0.625107 8:-70686980 None:intergenic
AACCGACACAGAGCTTGTTG+TGG 0.631106 8:+70687257 MsG0880046219.01.T01:CDS
TCACAGGGGCGAAGAATGTG+AGG 0.634295 8:+70687343 MsG0880046219.01.T01:CDS
GAGTGTTAAGAAGAAACTTG+AGG 0.637701 8:+70687446 MsG0880046219.01.T01:CDS
CACCACAACAAGCTCTGTGT+CGG 0.640807 8:-70687259 None:intergenic
CCCAGAATTCCCATACCTGA+CGG 0.678407 8:-70687406 None:intergenic
TGTGTGTGGTTGGAAGCTAG+AGG 0.683601 8:-70687145 None:intergenic
AGAAATAGTCGAACACCTTG+GGG 0.687282 8:-70687065 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAACAGGTTCAAAAACTCAC+AGG + Chr8:70687327-70687346 MsG0880046219.01.T01:CDS 35.0%
AACAGGTTCAAAAACTCACA+GGG + Chr8:70687328-70687347 MsG0880046219.01.T01:CDS 35.0%
AATTGCTGCTCAACTTGATT+TGG + Chr8:70687470-70687489 MsG0880046219.01.T01:CDS 35.0%
ATTGCTGCTCAACTTGATTT+GGG + Chr8:70687471-70687490 MsG0880046219.01.T01:CDS 35.0%
GAGTGTTAAGAAGAAACTTG+AGG + Chr8:70687446-70687465 MsG0880046219.01.T01:CDS 35.0%
GTTGTTGGATTTGGCTAAAT+CGG - Chr8:70686992-70687011 None:intergenic 35.0%
AACTGCTCTTGATCCAAGTT+TGG + Chr8:70687371-70687390 MsG0880046219.01.T01:CDS 40.0%
ACAGGTTCAAAAACTCACAG+GGG + Chr8:70687329-70687348 MsG0880046219.01.T01:CDS 40.0%
AGAAATAGTCGAACACCTTG+GGG - Chr8:70687068-70687087 None:intergenic 40.0%
GAAATTGGAGTCACAGCATT+TGG + Chr8:70687293-70687312 MsG0880046219.01.T01:CDS 40.0%
GAGAAATAGTCGAACACCTT+GGG - Chr8:70687069-70687088 None:intergenic 40.0%
TGGTGGAGTCTTCGATAATT+GGG - Chr8:70687095-70687114 None:intergenic 40.0%
TTGGTGGAGTCTTCGATAAT+TGG - Chr8:70687096-70687115 None:intergenic 40.0%
TTTGGCTAAATCGGCTAATG+CGG - Chr8:70686983-70687002 None:intergenic 40.0%
! TTCGATTTTGTCGATGTCAG+CGG - Chr8:70687031-70687050 None:intergenic 40.0%
!! TTGAGGAAGTTTTGTGTGTG+TGG - Chr8:70687162-70687181 None:intergenic 40.0%
CGATTCTGTTTGCTTCGATG+AGG - Chr8:70687188-70687207 None:intergenic 45.0%
GGAGAAATAGTCGAACACCT+TGG - Chr8:70687070-70687089 None:intergenic 45.0%
GGTGTCTTCAGTGGTGAAAT+TGG + Chr8:70687278-70687297 MsG0880046219.01.T01:CDS 45.0%
TGCTCTTGATCCAAGTTTGG+TGG + Chr8:70687374-70687393 MsG0880046219.01.T01:CDS 45.0%
TTCAGCGATGAGTTTGCGTT+TGG - Chr8:70687115-70687134 None:intergenic 45.0%
TTCTGTTTGCTTCGATGAGG+AGG - Chr8:70687185-70687204 None:intergenic 45.0%
! TCTTGATCCAAGTTTGGTGG+CGG + Chr8:70687377-70687396 MsG0880046219.01.T01:CDS 45.0%
!! GGAAGTTTTGTGTGTGTGGT+TGG - Chr8:70687158-70687177 None:intergenic 45.0%
ACCGTCAGGTATGGGAATTC+TGG + Chr8:70687405-70687424 MsG0880046219.01.T01:CDS 50.0%
AGAACTCTTCACTGACCCCA+AGG + Chr8:70687050-70687069 MsG0880046219.01.T01:CDS 50.0%
AGCGATGAGTTTGCGTTTGG+TGG - Chr8:70687112-70687131 None:intergenic 50.0%
CACCACAACAAGCTCTGTGT+CGG - Chr8:70687262-70687281 None:intergenic 50.0%
CCCAGAATTCCCATACCTGA+CGG - Chr8:70687409-70687428 None:intergenic 50.0%
CCGTCAGGTATGGGAATTCT+GGG + Chr8:70687406-70687425 MsG0880046219.01.T01:CDS 50.0%
CGAATTCTCTCACGATGTCG+AGG - Chr8:70687218-70687237 None:intergenic 50.0%
GGAGTCTTCGATAATTGGGC+TGG - Chr8:70687091-70687110 None:intergenic 50.0%
GTAAAACCCGCCACCAAACT+TGG - Chr8:70687387-70687406 None:intergenic 50.0%
TGTGTGTGGTTGGAAGCTAG+AGG - Chr8:70687148-70687167 None:intergenic 50.0%
TTGCTTCGATGAGGAGGTTG+AGG - Chr8:70687179-70687198 None:intergenic 50.0%
TTTGGCGCAGATTGCGAAAC+AGG + Chr8:70687311-70687330 MsG0880046219.01.T01:CDS 50.0%
! CGGGTTTTACCGTCAGGTAT+GGG + Chr8:70687397-70687416 MsG0880046219.01.T01:CDS 50.0%
! CTTGATCCAAGTTTGGTGGC+GGG + Chr8:70687378-70687397 MsG0880046219.01.T01:CDS 50.0%
! GATTTTGTCGATGTCAGCGG+TGG - Chr8:70687028-70687047 None:intergenic 50.0%
!! AACCGACACAGAGCTTGTTG+TGG + Chr8:70687257-70687276 MsG0880046219.01.T01:CDS 50.0%
!! GCTTGTTGTGGTGTCTTCAG+TGG + Chr8:70687269-70687288 MsG0880046219.01.T01:CDS 50.0%
!! GTCGAGGGTGTTGTTGGATT+TGG - Chr8:70687001-70687020 None:intergenic 50.0%
GGCTAAATCGGCTAATGCGG+TGG - Chr8:70686980-70686999 None:intergenic 55.0%
TCACAGGGGCGAAGAATGTG+AGG + Chr8:70687343-70687362 MsG0880046219.01.T01:CDS 55.0%
TTTGTCGATGTCAGCGGTGG+TGG - Chr8:70687025-70687044 None:intergenic 55.0%
! GCGGGTTTTACCGTCAGGTA+TGG + Chr8:70687396-70687415 MsG0880046219.01.T01:CDS 55.0%
TAATGCGGTGGCGTAGCTGG+CGG - Chr8:70686968-70686987 None:intergenic 60.0%
!!! TGGTGGCGGGTTTTACCGTC+AGG + Chr8:70687391-70687410 MsG0880046219.01.T01:CDS 60.0%
GGCTAATGCGGTGGCGTAGC+TGG - Chr8:70686971-70686990 None:intergenic 65.0%
!! GGTGGCGTCGAGGGTGTTGT+TGG - Chr8:70687007-70687026 None:intergenic 65.0%
! GTCAGCGGTGGTGGCGTCGA+GGG - Chr8:70687016-70687035 None:intergenic 70.0%
! TGTCAGCGGTGGTGGCGTCG+AGG - Chr8:70687017-70687036 None:intergenic 70.0%
TGCGGTGGCGTAGCTGGCGG+CGG - Chr8:70686965-70686984 None:intergenic 75.0%
TGGCGTAGCTGGCGGCGGCT+GGG - Chr8:70686960-70686979 None:intergenic 75.0%
! GGCGTAGCTGGCGGCGGCTG+GGG - Chr8:70686959-70686978 None:intergenic 80.0%
! GTGGCGTAGCTGGCGGCGGC+TGG - Chr8:70686961-70686980 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr8 gene 70686949 70687515 70686949 ID=MsG0880046219.01;Name=MsG0880046219.01
Chr8 mRNA 70686949 70687515 70686949 ID=MsG0880046219.01.T01;Parent=MsG0880046219.01;Name=MsG0880046219.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|188
Chr8 exon 70686949 70687515 70686949 ID=MsG0880046219.01.T01:exon:27674;Parent=MsG0880046219.01.T01
Chr8 CDS 70686949 70687515 70686949 ID=MsG0880046219.01.T01:cds;Parent=MsG0880046219.01.T01
Gene Sequence

>MsG0880046219.01.T01

ATGAACTCCCCAGCCGCCGCCAGCTACGCCACCGCATTAGCCGATTTAGCCAAATCCAACAACACCCTCGACGCCACCACCGCTGACATCGACAAAATCGAAGAACTCTTCACTGACCCCAAGGTGTTCGACTATTTCTCCAGCCCAATTATCGAAGACTCCACCAAACGCAAACTCATCGCTGAATTCGCTACTTCCTCTAGCTTCCAACCACACACACAAAACTTCCTCAACCTCCTCATCGAAGCAAACAGAATCGACATCATCCTCGACATCGTGAGAGAATTCGAACTTCATTACAATACTCTAACCGACACAGAGCTTGTTGTGGTGTCTTCAGTGGTGAAATTGGAGTCACAGCATTTGGCGCAGATTGCGAAACAGGTTCAAAAACTCACAGGGGCGAAGAATGTGAGGATTAAAACTGCTCTTGATCCAAGTTTGGTGGCGGGTTTTACCGTCAGGTATGGGAATTCTGGGTCTAAGTTTATTGATATGAGTGTTAAGAAGAAACTTGAGGAAATTGCTGCTCAACTTGATTTGGGTGATATCAAACTTGCTGCATGA

Protein sequence

>MsG0880046219.01.T01

MNSPAAASYATALADLAKSNNTLDATTADIDKIEELFTDPKVFDYFSSPIIEDSTKRKLIAEFATSSSFQPHTQNFLNLLIEANRIDIILDIVREFELHYNTLTDTELVVVSSVVKLESQHLAQIAKQVQKLTGAKNVRIKTALDPSLVAGFTVRYGNSGSKFIDMSVKKKLEEIAAQLDLGDIKLAA*