AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042412.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042412.01.T01 MTR_8g011470 99.306 144 1 0 7 150 3 146 1.60e-107 302
MsG0880042412.01.T01 MTR_8g011470 99.225 129 1 0 7 135 3 131 1.69e-94 269
MsG0880042412.01.T01 MTR_7g052620 55.629 151 61 4 6 150 1 151 1.71e-56 174
MsG0880042412.01.T01 MTR_7g116610 34.783 161 81 6 12 150 3 161 2.99e-19 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042412.01.T01 AT5G48500 54.286 140 48 8 12 137 12 149 7.60e-40 132

Find 35 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGAAAATATAATATTTA+AGG 0.085777 8:+8378448 MsG0880042412.01.T01:three_prime_UTR
CTATAATATGATAATCTTCT+TGG 0.285873 8:-8378472 None:intergenic
AGCTATGTTACCAGAAATTA+AGG 0.293215 8:+8378630 MsG0880042412.01.T01:three_prime_UTR
TGATTGTTGTTCCACGTAGA+AGG 0.326423 8:+8376660 MsG0880042412.01.T01:CDS
AGGAACCATGTACCAGCATT+TGG 0.399058 8:+8376482 MsG0880042412.01.T01:CDS
TTGTACAAGTCTTCATCAAC+AGG 0.401129 8:-8378036 None:intergenic
GATCAAGATCTTTATGTTGC+TGG 0.401416 8:+8376602 MsG0880042412.01.T01:CDS
TGATTATTGTGCTAAGCTCT+TGG 0.402533 8:+8378500 MsG0880042412.01.T01:three_prime_UTR
CTTTGAGGTGTATCATTGAT+TGG 0.403387 8:+8378586 MsG0880042412.01.T01:three_prime_UTR
TTAGTCTTTGATGTACTTTG+AGG 0.416023 8:+8378571 MsG0880042412.01.T01:three_prime_UTR
CAGCATTTGGAAGCTGGGAT+TGG 0.423755 8:+8376495 MsG0880042412.01.T01:CDS
GATTCAAAGCATTGAGTGAA+TGG 0.443836 8:-8376530 None:intergenic
TCATCAACAGGCTTTGGTGT+TGG 0.446438 8:-8378024 None:intergenic
ATGTACCAGCATTTGGAAGC+TGG 0.447529 8:+8376489 MsG0880042412.01.T01:CDS
CGTGGAACAACAATCATTGC+AGG 0.459147 8:-8376653 None:intergenic
CTTGACATCGGAGTAGGACT+TGG 0.462136 8:-8378000 None:intergenic
AAGTCTTCATCAACAGGCTT+TGG 0.463769 8:-8378030 None:intergenic
GTACATGGTTCCTCTTGAAA+TGG 0.466033 8:-8376472 None:intergenic
GGTACGTTCCCAACATGAAA+AGG 0.486529 8:+8377933 MsG0880042412.01.T01:CDS
ATAGTAGATAGATACTCTCT+TGG 0.491827 8:+8378415 MsG0880042412.01.T01:three_prime_UTR
CAATCCCAGCTTCCAAATGC+TGG 0.499013 8:-8376494 None:intergenic
TGTACCAGCATTTGGAAGCT+GGG 0.509582 8:+8376490 MsG0880042412.01.T01:CDS
AGCTTCCAAATGCTGGTACA+TGG 0.514670 8:-8376487 None:intergenic
GACATCGGAGTAGGACTTGG+TGG 0.521886 8:-8377997 None:intergenic
GATTGTTGTTCCACGTAGAA+GGG 0.539249 8:+8376661 MsG0880042412.01.T01:CDS
TTGGTGGTTCTTTCACAACA+TGG 0.545398 8:-8377981 None:intergenic
TTTGGTGTTGGTCTTGACAT+CGG 0.551558 8:-8378012 None:intergenic
ATCTTCTTTATGTCAAAACT+AGG 0.563542 8:+8378070 MsG0880042412.01.T01:CDS
GTTGGTCTTGACATCGGAGT+AGG 0.572189 8:-8378006 None:intergenic
TCTTTATGTCAAAACTAGGA+AGG 0.584419 8:+8378074 MsG0880042412.01.T01:CDS
TGCTTTGAATCTGCAAGACA+AGG 0.600221 8:+8376542 MsG0880042412.01.T01:CDS
AATAGGAATACCATTTCAAG+AGG 0.628897 8:+8376462 MsG0880042412.01.T01:intron
ATGAGTCAAGAAAATTTGAG+AGG 0.637897 8:+8378351 MsG0880042412.01.T01:three_prime_UTR
TTTGAATCTGCAAGACAAGG+TGG 0.656042 8:+8376545 MsG0880042412.01.T01:CDS
AGGTGACATACCCTTCTACG+TGG 0.761356 8:-8376671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATTAATTTCTCTA+TGG + Chr8:8377182-8377201 MsG0880042412.01.T01:intron 10.0%
!! AAATAAAAATGATTAAATGA+AGG + Chr8:8378384-8378403 MsG0880042412.01.T01:three_prime_UTR 10.0%
!! TGTATATTAGTAAAAAAATA+TGG - Chr8:8377441-8377460 None:intergenic 10.0%
!!! AAATGAAAATTTAATTTTAG+TGG - Chr8:8378137-8378156 None:intergenic 10.0%
!!! ATCATTTTTATTTACAATAT+TGG - Chr8:8378377-8378396 None:intergenic 10.0%
!!! ATTGTATTTTTTTTTTTTTG+AGG + Chr8:8377311-8377330 MsG0880042412.01.T01:intron 10.0%
!!! TAATTATTTTTCATTAACAA+AGG - Chr8:8377173-8377192 None:intergenic 10.0%
!!! TTGTATTTTTTTTTTTTTGA+GGG + Chr8:8377312-8377331 MsG0880042412.01.T01:intron 10.0%
!! AAAAAAAGTGATATTAGAAA+AGG - Chr8:8378270-8378289 None:intergenic 15.0%
!! TGGATTTAAATCATTAATTA+GGG + Chr8:8377011-8377030 MsG0880042412.01.T01:intron 15.0%
!! TTGGATTTAAATCATTAATT+AGG + Chr8:8377010-8377029 MsG0880042412.01.T01:intron 15.0%
!!! AATTTTAATAGTGATAAAGT+AGG + Chr8:8377641-8377660 MsG0880042412.01.T01:intron 15.0%
!!! ATGAAAACTAAATGTTTTAT+GGG + Chr8:8376413-8376432 MsG0880042412.01.T01:intron 15.0%
!!! CATCATTTTATTTTATCTAT+AGG - Chr8:8376903-8376922 None:intergenic 15.0%
!!! TATGAAAACTAAATGTTTTA+TGG + Chr8:8376412-8376431 MsG0880042412.01.T01:intron 15.0%
!! AAATATGGTCAAAGTTAAAT+AGG - Chr8:8377426-8377445 None:intergenic 20.0%
!! ATAATTAATTTCTCTATGGT+AGG + Chr8:8377186-8377205 MsG0880042412.01.T01:intron 20.0%
!! ATAATTGAATATGCACTAAT+AGG - Chr8:8378193-8378212 None:intergenic 20.0%
!! ATCATTAATGTCAATACATT+AGG - Chr8:8377236-8377255 None:intergenic 20.0%
!! CTATAATATGATAATCTTCT+TGG - Chr8:8378475-8378494 None:intergenic 20.0%
!! TTAATAATGCATCTAACTTT+AGG + Chr8:8377681-8377700 MsG0880042412.01.T01:intron 20.0%
!! TTTATAAAAAGTGACATTTG+TGG - Chr8:8377871-8377890 None:intergenic 20.0%
!!! ATATATTGATTGTCAAAGTT+AGG + Chr8:8376768-8376787 MsG0880042412.01.T01:intron 20.0%
!!! GTTTTAATTTTATCCTCAAT+AGG + Chr8:8377047-8377066 MsG0880042412.01.T01:intron 20.0%
!!! TAATTTGTTGTTAACATTGT+AGG + Chr8:8377906-8377925 MsG0880042412.01.T01:intron 20.0%
!!! TATATTGATTGTCAAAGTTA+GGG + Chr8:8376769-8376788 MsG0880042412.01.T01:intron 20.0%
!!! TGAAAACTAAATGTTTTATG+GGG + Chr8:8376414-8376433 MsG0880042412.01.T01:intron 20.0%
!!! TTTCTTCTTTTTCTCTTAAA+CGG + Chr8:8378530-8378549 MsG0880042412.01.T01:three_prime_UTR 20.0%
! ATCTTCTTTATGTCAAAACT+AGG + Chr8:8378070-8378089 MsG0880042412.01.T01:CDS 25.0%
! ATTAATGTCAATACATTAGG+AGG - Chr8:8377233-8377252 None:intergenic 25.0%
! CCAGAAATTAAGGAAAAAAA+AGG + Chr8:8378640-8378659 MsG0880042412.01.T01:three_prime_UTR 25.0%
! TAAATTGTATTGCATCAAAG+TGG + Chr8:8377102-8377121 MsG0880042412.01.T01:intron 25.0%
! TCACCAAAAATATAGACAAT+GGG + Chr8:8377726-8377745 MsG0880042412.01.T01:intron 25.0%
! TGCATTATTAAACAAAGTCA+TGG - Chr8:8377672-8377691 None:intergenic 25.0%
! TTATGAAAGCAAAGTAGTAA+AGG - Chr8:8378161-8378180 None:intergenic 25.0%
!! AAAACTAAATGTTTTATGGG+GGG + Chr8:8376416-8376435 MsG0880042412.01.T01:intron 25.0%
!! ATCTTGTTATGATATACACA+TGG - Chr8:8377754-8377773 None:intergenic 25.0%
!! GAAAACTAAATGTTTTATGG+GGG + Chr8:8376415-8376434 MsG0880042412.01.T01:intron 25.0%
!! TCTTTCTTTTTCTACTCTTA+CGG - Chr8:8377143-8377162 None:intergenic 25.0%
!!! CCTTTTTTTTCCTTAATTTC+TGG - Chr8:8378643-8378662 None:intergenic 25.0%
!!! CGTTTCTTATTTTCTAAAAG+AGG + Chr8:8376726-8376745 MsG0880042412.01.T01:intron 25.0%
!!! TTGTTTTGCATGTCTTTAAT+TGG + Chr8:8376986-8377005 MsG0880042412.01.T01:intron 25.0%
AATAGGAATACCATTTCAAG+AGG + Chr8:8376462-8376481 MsG0880042412.01.T01:intron 30.0%
AGCTATGTTACCAGAAATTA+AGG + Chr8:8378630-8378649 MsG0880042412.01.T01:three_prime_UTR 30.0%
AGGATGTGAAAAAGCAAAAT+TGG + Chr8:8377953-8377972 MsG0880042412.01.T01:CDS 30.0%
AGGTCACACAACTTTATAAT+TGG + Chr8:8377206-8377225 MsG0880042412.01.T01:intron 30.0%
ATAGTAGATAGATACTCTCT+TGG + Chr8:8378415-8378434 MsG0880042412.01.T01:three_prime_UTR 30.0%
ATGAGTCAAGAAAATTTGAG+AGG + Chr8:8378351-8378370 MsG0880042412.01.T01:three_prime_UTR 30.0%
GGATGTGAAAAAGCAAAATT+GGG + Chr8:8377954-8377973 MsG0880042412.01.T01:CDS 30.0%
GGTCACACAACTTTATAATT+GGG + Chr8:8377207-8377226 MsG0880042412.01.T01:intron 30.0%
GTCACCAAAAATATAGACAA+TGG + Chr8:8377725-8377744 MsG0880042412.01.T01:intron 30.0%
GTTGTTAACATTGTAGGAAA+AGG + Chr8:8377912-8377931 MsG0880042412.01.T01:intron 30.0%
TATTGCATCAAAGTGGTTAT+AGG + Chr8:8377109-8377128 MsG0880042412.01.T01:intron 30.0%
TCTTTATGTCAAAACTAGGA+AGG + Chr8:8378074-8378093 MsG0880042412.01.T01:CDS 30.0%
TTGCATGTCTTTAATTGGAT+TGG + Chr8:8376991-8377010 MsG0880042412.01.T01:intron 30.0%
TTTAAATAGGTCATCTATCG+CGG - Chr8:8377388-8377407 None:intergenic 30.0%
! GATCCCATTGTCTATATTTT+TGG - Chr8:8377732-8377751 None:intergenic 30.0%
! GCTTTATTGTCTCTGTTAAT+GGG + Chr8:8377703-8377722 MsG0880042412.01.T01:intron 30.0%
! TTAGTCTTTGATGTACTTTG+AGG + Chr8:8378571-8378590 MsG0880042412.01.T01:three_prime_UTR 30.0%
! TTTCACATCCTTTTCATGTT+GGG - Chr8:8377944-8377963 None:intergenic 30.0%
! TTTTCACATCCTTTTCATGT+TGG - Chr8:8377945-8377964 None:intergenic 30.0%
!! GTTTTGACATAAAGAAGATC+TGG - Chr8:8378069-8378088 None:intergenic 30.0%
!! TTTTTATTTGGGGGTGTAAT+AGG + Chr8:8376445-8376464 MsG0880042412.01.T01:intron 30.0%
ACGAAAACAGAGAAAAGACA+AGG - Chr8:8376709-8376728 None:intergenic 35.0%
AGACAAGGATAAGAGAGTTA+AGG - Chr8:8376694-8376713 None:intergenic 35.0%
AGTGGAGAAAAAGCAAAAGA+GGG - Chr8:8378119-8378138 None:intergenic 35.0%
GATCAAGATCTTTATGTTGC+TGG + Chr8:8376602-8376621 MsG0880042412.01.T01:CDS 35.0%
TAGTGGAGAAAAAGCAAAAG+AGG - Chr8:8378120-8378139 None:intergenic 35.0%
TCAGCTGTGTCAAGTATTAA+GGG + Chr8:8377599-8377618 MsG0880042412.01.T01:intron 35.0%
TGATTATTGTGCTAAGCTCT+TGG + Chr8:8378500-8378519 MsG0880042412.01.T01:three_prime_UTR 35.0%
TTAAATAGGTCATCTATCGC+GGG - Chr8:8377387-8377406 None:intergenic 35.0%
TTGTACAAGTCTTCATCAAC+AGG - Chr8:8378039-8378058 None:intergenic 35.0%
! ATGCATTGACATGCGTAATA+AGG + Chr8:8377576-8377595 MsG0880042412.01.T01:intron 35.0%
!! CTTTGAGGTGTATCATTGAT+TGG + Chr8:8378586-8378605 MsG0880042412.01.T01:three_prime_UTR 35.0%
!! GATTCAAAGCATTGAGTGAA+TGG - Chr8:8376533-8376552 None:intergenic 35.0%
!! GGCTTTATTGTCTCTGTTAA+TGG + Chr8:8377702-8377721 MsG0880042412.01.T01:intron 35.0%
AAGTCTTCATCAACAGGCTT+TGG - Chr8:8378033-8378052 None:intergenic 40.0%
ATGTACAGCAACATAACTCG+CGG + Chr8:8376920-8376939 MsG0880042412.01.T01:intron 40.0%
CTGTGTCAAGTATTAAGGGA+CGG + Chr8:8377603-8377622 MsG0880042412.01.T01:intron 40.0%
GGGAATCTATCACTCTATGT+TGG + Chr8:8376789-8376808 MsG0880042412.01.T01:intron 40.0%
GTACATGGTTCCTCTTGAAA+TGG - Chr8:8376475-8376494 None:intergenic 40.0%
GTCAGCTGTGTCAAGTATTA+AGG + Chr8:8377598-8377617 MsG0880042412.01.T01:intron 40.0%
TTTGAATCTGCAAGACAAGG+TGG + Chr8:8376545-8376564 MsG0880042412.01.T01:CDS 40.0%
TTTGCACACTCTTCCTATTG+AGG - Chr8:8377063-8377082 None:intergenic 40.0%
! AAAAAGCAAAAGAGGGTGTG+TGG - Chr8:8378112-8378131 None:intergenic 40.0%
! AAAAGCAAAAGAGGGTGTGT+GGG - Chr8:8378111-8378130 None:intergenic 40.0%
! GATTGTTGTTCCACGTAGAA+GGG + Chr8:8376661-8376680 MsG0880042412.01.T01:CDS 40.0%
! TAAATGTTTTATGGGGGGTG+TGG + Chr8:8376421-8376440 MsG0880042412.01.T01:intron 40.0%
! TGATTGTTGTTCCACGTAGA+AGG + Chr8:8376660-8376679 MsG0880042412.01.T01:CDS 40.0%
! TGCTTTGAATCTGCAAGACA+AGG + Chr8:8376542-8376561 MsG0880042412.01.T01:CDS 40.0%
! TTGGTGGTTCTTTCACAACA+TGG - Chr8:8377984-8378003 None:intergenic 40.0%
! TTTTGCCAATGCAGAAGAGA+GGG + Chr8:8378287-8378306 MsG0880042412.01.T01:intron 40.0%
! TTTTTGCCAATGCAGAAGAG+AGG + Chr8:8378286-8378305 MsG0880042412.01.T01:intron 40.0%
!! TTTGGTGTTGGTCTTGACAT+CGG - Chr8:8378015-8378034 None:intergenic 40.0%
!!! GGGGGTGTGGTTTTTTATTT+GGG + Chr8:8376434-8376453 MsG0880042412.01.T01:intron 40.0%
!!! GGGGTGTGGTTTTTTATTTG+GGG + Chr8:8376435-8376454 MsG0880042412.01.T01:intron 40.0%
!!! GGGTGTGGTTTTTTATTTGG+GGG + Chr8:8376436-8376455 MsG0880042412.01.T01:intron 40.0%
AACTCGCGGTAAGTAACACA+TGG + Chr8:8376934-8376953 MsG0880042412.01.T01:intron 45.0%
ACTCGCGGTAAGTAACACAT+GGG + Chr8:8376935-8376954 MsG0880042412.01.T01:intron 45.0%
AGCTTCCAAATGCTGGTACA+TGG - Chr8:8376490-8376509 None:intergenic 45.0%
AGGAACCATGTACCAGCATT+TGG + Chr8:8376482-8376501 MsG0880042412.01.T01:CDS 45.0%
ATGTACCAGCATTTGGAAGC+TGG + Chr8:8376489-8376508 MsG0880042412.01.T01:CDS 45.0%
CGTGGAACAACAATCATTGC+AGG - Chr8:8376656-8376675 None:intergenic 45.0%
GGTACGTTCCCAACATGAAA+AGG + Chr8:8377933-8377952 MsG0880042412.01.T01:CDS 45.0%
GTCAAGTATTAAGGGACGGA+GGG + Chr8:8377607-8377626 MsG0880042412.01.T01:intron 45.0%
TGTACCAGCATTTGGAAGCT+GGG + Chr8:8376490-8376509 MsG0880042412.01.T01:CDS 45.0%
TGTCAAGTATTAAGGGACGG+AGG + Chr8:8377606-8377625 MsG0880042412.01.T01:intron 45.0%
! AAAGCAAAAGAGGGTGTGTG+GGG - Chr8:8378110-8378129 None:intergenic 45.0%
! TCATCAACAGGCTTTGGTGT+TGG - Chr8:8378027-8378046 None:intergenic 45.0%
!!! GGGGGGTGTGGTTTTTTATT+TGG + Chr8:8376433-8376452 MsG0880042412.01.T01:intron 45.0%
!! ATTTGAAAATATAATATTTA+AGG + Chr8:8378448-8378467 MsG0880042412.01.T01:three_prime_UTR 5.0%
!!! AAAATTAAACATTTTTAAAT+AGG - Chr8:8377401-8377420 None:intergenic 5.0%
!!! TATAAAATTAATAAAACTTT+TGG - Chr8:8378242-8378261 None:intergenic 5.0%
AGGTGACATACCCTTCTACG+TGG - Chr8:8376674-8376693 None:intergenic 50.0%
ATAGGTCATCTATCGCGGGA+CGG - Chr8:8377383-8377402 None:intergenic 50.0%
CAATCCCAGCTTCCAAATGC+TGG - Chr8:8376497-8376516 None:intergenic 50.0%
CACAACCCTCTCTTCTGCAT+TGG - Chr8:8378295-8378314 None:intergenic 50.0%
CAGCATTTGGAAGCTGGGAT+TGG + Chr8:8376495-8376514 MsG0880042412.01.T01:CDS 50.0%
CTTGACATCGGAGTAGGACT+TGG - Chr8:8378003-8378022 None:intergenic 50.0%
!! GTTGGTCTTGACATCGGAGT+AGG - Chr8:8378009-8378028 None:intergenic 50.0%
GACATCGGAGTAGGACTTGG+TGG - Chr8:8378000-8378019 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 8376375 8378669 8376375 ID=MsG0880042412.01;Name=MsG0880042412.01
Chr8 mRNA 8376375 8378669 8376375 ID=MsG0880042412.01.T01;Parent=MsG0880042412.01;Name=MsG0880042412.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0.66|0.75|1|0.33|0.25|4|321|150
Chr8 exon 8378301 8378669 8378301 ID=MsG0880042412.01.T01:exon:1114;Parent=MsG0880042412.01.T01
Chr8 exon 8377928 8378095 8377928 ID=MsG0880042412.01.T01:exon:1113;Parent=MsG0880042412.01.T01
Chr8 exon 8376467 8376682 8376467 ID=MsG0880042412.01.T01:exon:1112;Parent=MsG0880042412.01.T01
Chr8 exon 8376375 8376395 8376375 ID=MsG0880042412.01.T01:exon:1111;Parent=MsG0880042412.01.T01
Chr8 CDS 8376375 8376395 8376375 ID=MsG0880042412.01.T01:cds;Parent=MsG0880042412.01.T01
Chr8 CDS 8376467 8376682 8376467 ID=MsG0880042412.01.T01:cds;Parent=MsG0880042412.01.T01
Chr8 CDS 8377928 8378095 8377928 ID=MsG0880042412.01.T01:cds;Parent=MsG0880042412.01.T01
Chr8 CDS 8378301 8378348 8378301 ID=MsG0880042412.01.T01:cds;Parent=MsG0880042412.01.T01
Chr8 three_prime_UTR 8378349 8378669 8378349 ID=MsG0880042412.01.T01:three_prime_utr;Parent=MsG0880042412.01.T01
Gene Sequence

>MsG0880042412.01.T01

ATGTTCTTTGTTGATATTAATGAATACCATTTCAAGAGGAACCATGTACCAGCATTTGGAAGCTGGGATTGGAATGATAATCTTCCATTCACTCAATGCTTTGAATCTGCAAGACAAGGTGGTTTTCTACACTATAGTTACTCTGAAAGTAATGAAGATCAAGATCTTTATGTTGCTGGTGATTTGTATGATAATCATGTTGTTACTCCTGCAATGATTGTTGTTCCACGTAGAAGGGAAAAGGTACGTTCCCAACATGAAAAGGATGTGAAAAAGCAAAATTGGGTGAGCCATGTTGTGAAAGAACCACCAAGTCCTACTCCGATGTCAAGACCAACACCAAAGCCTGTTGATGAAGACTTGTACAAAATTTCACCAGATCTTCTTTATGTCAAAACTAGGAAGAAGAGAGGGTTGTGTTTCTTTCAAAGTTGCTTCACTTGCATTGCATGA

Protein sequence

>MsG0880042412.01.T01

MFFVDINEYHFKRNHVPAFGSWDWNDNLPFTQCFESARQGGFLHYSYSESNEDQDLYVAGDLYDNHVVTPAMIVVPRRREKVRSQHEKDVKKQNWVSHVVKEPPSPTPMSRPTPKPVDEDLYKISPDLLYVKTRKKRGLCFFQSCFTCIA*