AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047681.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047681.01.T01 MTR_4g132760 92.000 225 10 4 1 225 1 217 6.86e-136 380
MsG0880047681.01.T01 MTR_2g025080 54.327 208 63 7 1 198 1 186 3.88e-56 177
MsG0880047681.01.T01 MTR_8g013650 83.673 49 8 0 177 225 186 234 2.44e-26 102
MsG0880047681.01.T01 MTR_7g056407 44.231 104 39 2 140 225 237 339 9.00e-21 89.4
MsG0880047681.01.T01 MTR_3g065180 60.000 50 20 0 176 225 291 340 5.28e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047681.01.T01 AT4G03965 47.679 237 84 7 1 225 1 209 8.04e-58 182
MsG0880047681.01.T01 AT4G22250 48.131 214 80 7 21 225 23 214 3.17e-53 171
MsG0880047681.01.T01 AT1G62370 40.851 235 98 8 1 225 1 204 5.29e-44 147
MsG0880047681.01.T01 AT3G25030 53.448 116 46 2 117 225 96 210 6.59e-35 124
MsG0880047681.01.T01 AT3G25030 53.448 116 46 2 117 225 136 250 2.61e-34 123
MsG0880047681.01.T01 AT3G25030 53.448 116 46 2 117 225 136 250 2.61e-34 123
MsG0880047681.01.T01 AT3G25030 53.448 116 46 2 117 225 136 250 2.61e-34 123
MsG0880047681.01.T01 AT4G13100 50.394 127 32 4 117 225 146 259 1.60e-29 111
MsG0880047681.01.T01 AT4G13100 50.394 127 32 4 117 225 146 259 1.60e-29 111
MsG0880047681.01.T01 AT4G13100 50.394 127 32 4 117 225 146 259 1.60e-29 111
MsG0880047681.01.T01 AT4G13100 50.394 127 32 4 117 225 146 259 1.60e-29 111
MsG0880047681.01.T01 AT4G13100 50.394 127 32 4 117 225 143 256 1.70e-29 110
MsG0880047681.01.T01 AT4G13100 49.606 127 30 4 117 225 146 256 2.36e-29 110
MsG0880047681.01.T01 AT4G13100 49.606 127 30 4 117 225 146 256 2.36e-29 110
MsG0880047681.01.T01 AT4G13100 49.606 127 30 4 117 225 155 265 3.50e-29 110
MsG0880047681.01.T01 AT3G07120 59.184 49 20 0 177 225 312 360 7.39e-17 78.6

Find 73 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTTCGTTGCGGATAAT+TGG 0.249983 8:-89589066 None:intergenic
GTTTCACATTCCGGTTCAAC+TGG 0.280636 8:-89589180 None:intergenic
GTGGTGGGTTCGTCTGGTTC+TGG 0.300899 8:+89589207 MsG0880047681.01.T01:CDS
TTCTTTGAGACTACGTCTTC+TGG 0.310742 8:-89588992 None:intergenic
TGGGTTGTTGTGGTGCCACC+TGG 0.326153 8:+89589034 MsG0880047681.01.T01:CDS
ACTCCGGTGGTGGGTTCGTC+TGG 0.370885 8:+89589201 MsG0880047681.01.T01:CDS
CCTTGCGGCGGCGTTGGCGG+CGG 0.381107 8:+89589236 MsG0880047681.01.T01:CDS
CAAATGGACAGTTTAGATTC+AGG 0.383260 8:+89588964 MsG0880047681.01.T01:CDS
CGCTTCAGATTCACCGGTAT+GGG 0.387954 8:+89589015 MsG0880047681.01.T01:CDS
CGGTTTATTAACCGAACAAA+TGG 0.401011 8:+89588948 MsG0880047681.01.T01:CDS
CCGCCGCCAACGCCGCCGCA+AGG 0.401311 8:-89589236 None:intergenic
CATTCCGGTTCAACTGGGTT+CGG 0.404037 8:-89589174 None:intergenic
ATCTAAACTGTCCATTTGTT+CGG 0.404178 8:-89588959 None:intergenic
GAACCTTGCGGCGGCGTTGG+CGG 0.404942 8:+89589233 MsG0880047681.01.T01:CDS
TTCGGTTAATAAACCGAGAA+TGG 0.411730 8:-89588941 None:intergenic
GCTAGTGGTGGTGGCGGAGA+AGG 0.413073 8:+89589285 MsG0880047681.01.T01:CDS
TCAACTCGGTCTCATATTGC+AGG 0.414710 8:+89588888 MsG0880047681.01.T01:CDS
ACGGAGGAGGCTAGTGGTGG+TGG 0.422655 8:+89589276 MsG0880047681.01.T01:CDS
AGAGAATCAAGAACCATTCT+CGG 0.424957 8:+89588928 MsG0880047681.01.T01:CDS
AGATCCGAACCCAGTTGAAC+CGG 0.458025 8:+89589170 MsG0880047681.01.T01:CDS
AATGAACCTTGCGGCGGCGT+TGG 0.463100 8:+89589230 MsG0880047681.01.T01:CDS
ACCACCGGAGTTTCACATTC+CGG 0.463442 8:-89589189 None:intergenic
TAATGCTGGTGCGGTGGTTG+TGG 0.471853 8:+89589371 MsG0880047681.01.T01:CDS
TGGCGGCGGAGCGGCAAATG+AGG 0.476419 8:+89589250 MsG0880047681.01.T01:CDS
GAGAATAGAACGATTACAAA+GGG 0.479329 8:-89589517 None:intergenic
CGGCGGCGTTGGCGGCGGAG+CGG 0.486064 8:+89589241 MsG0880047681.01.T01:CDS
ACGCTTCAGATTCACCGGTA+TGG 0.495940 8:+89589014 MsG0880047681.01.T01:CDS
GCTGGTGCGGTGGTTGTGGA+AGG 0.512004 8:+89589375 MsG0880047681.01.T01:CDS
AGTTTCGTTGATGAAACTGT+TGG 0.512656 8:+89589323 MsG0880047681.01.T01:CDS
GAGGAGGCTAGTGGTGGTGG+CGG 0.517007 8:+89589279 MsG0880047681.01.T01:CDS
GATGAAACTGTTGGAGGAAA+CGG 0.519664 8:+89589332 MsG0880047681.01.T01:CDS
TTCGTTGATGAAACTGTTGG+AGG 0.522016 8:+89589326 MsG0880047681.01.T01:CDS
GGAATGTGAAACTCCGGTGG+TGG 0.529577 8:+89589191 MsG0880047681.01.T01:CDS
ACTGTTGGAGGAAACGGAAG+TGG 0.532838 8:+89589338 MsG0880047681.01.T01:CDS
GCAAATGAGGGTAACGGAGG+AGG 0.537713 8:+89589263 MsG0880047681.01.T01:CDS
CAACAACAACAAAACGAAGA+AGG 0.542306 8:+89589099 MsG0880047681.01.T01:CDS
GGCACCACAACAACCCATAC+CGG 0.553017 8:-89589028 None:intergenic
GGGTTGTTGTGGTGCCACCT+GGG 0.563855 8:+89589035 MsG0880047681.01.T01:CDS
CCGGTCAATATGAGTCAACT+CGG 0.576822 8:+89588874 None:intergenic
TTTCACATTCCGGTTCAACT+GGG 0.578697 8:-89589179 None:intergenic
GAACCAGACGAACCCACCAC+CGG 0.580808 8:-89589204 None:intergenic
GTGGAAAATGAGAGTAATGC+TGG 0.585452 8:+89589357 MsG0880047681.01.T01:CDS
AAAGAACGCTTCAGATTCAC+CGG 0.587827 8:+89589009 MsG0880047681.01.T01:CDS
GGCGGCGGAGCGGCAAATGA+GGG 0.590263 8:+89589251 MsG0880047681.01.T01:CDS
CGAGAATAGAACGATTACAA+AGG 0.598192 8:-89589518 None:intergenic
CTGGTGCGGTGGTTGTGGAA+GGG 0.603127 8:+89589376 MsG0880047681.01.T01:CDS
TTCACCGGTATGGGTTGTTG+TGG 0.607708 8:+89589024 MsG0880047681.01.T01:CDS
GGAGCGGCAAATGAGGGTAA+CGG 0.608763 8:+89589257 MsG0880047681.01.T01:CDS
CAACAACAAAACGAAGAAGG+TGG 0.612798 8:+89589102 MsG0880047681.01.T01:CDS
TGAACCGGAATGTGAAACTC+CGG 0.616093 8:+89589185 MsG0880047681.01.T01:CDS
GTTCGTTGCGGATAATTGGA+CGG 0.625036 8:-89589062 None:intergenic
GCGGAGAAGGAGCGACGCCG+TGG 0.628639 8:+89589298 MsG0880047681.01.T01:CDS
AGGGTAACGGAGGAGGCTAG+TGG 0.629298 8:+89589270 MsG0880047681.01.T01:CDS
GTAACGGAGGAGGCTAGTGG+TGG 0.631967 8:+89589273 MsG0880047681.01.T01:CDS
TTTGAGACTACGTCTTCTGG+TGG 0.634831 8:-89588989 None:intergenic
GAATGTGAAACTCCGGTGGT+GGG 0.635237 8:+89589192 MsG0880047681.01.T01:CDS
TTGTTGTTGTTGCTCCACTG+TGG 0.640686 8:-89589127 None:intergenic
GCGGCAAATGAGGGTAACGG+AGG 0.646014 8:+89589260 MsG0880047681.01.T01:CDS
GATAATTGGACGGAAAACCC+AGG 0.649559 8:-89589052 None:intergenic
CGAAGAAGGTGGAGCCACAG+TGG 0.650707 8:+89589113 MsG0880047681.01.T01:CDS
AGGGAACTGTGGTTGAATCG+AGG 0.655246 8:+89589486 MsG0880047681.01.T01:CDS
TTCATCAACGAAACTCTCCA+CGG 0.677889 8:-89589315 None:intergenic
GTGTGCATGAGAAGAAAGAA+AGG 0.679700 8:+89589417 MsG0880047681.01.T01:CDS
GATGATGATGATGTTCGTTG+CGG 0.682625 8:-89589074 None:intergenic
TGAGAGTAATGCTGGTGCGG+TGG 0.688076 8:+89589365 MsG0880047681.01.T01:CDS
GAGTGTGTTCGAGGGAACTG+TGG 0.692857 8:+89589475 MsG0880047681.01.T01:CDS
TTCTGGAATGAACCTTGCGG+CGG 0.694080 8:+89589224 MsG0880047681.01.T01:CDS
AAATGAGAGTAATGCTGGTG+CGG 0.699341 8:+89589362 MsG0880047681.01.T01:CDS
GGTGCAGCGTTCATACCGTG+TGG 0.706510 8:+89589438 MsG0880047681.01.T01:CDS
AGAATAGAACGATTACAAAG+GGG 0.720178 8:-89589516 None:intergenic
TGGTTCTGGAATGAACCTTG+CGG 0.740354 8:+89589221 MsG0880047681.01.T01:CDS
ACCGGAATGTGAAACTCCGG+TGG 0.748395 8:+89589188 MsG0880047681.01.T01:CDS
AATTGGACGGAAAACCCAGG+TGG 0.782059 8:-89589049 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAATAGAACGATTACAAAG+GGG - Chr8:89589519-89589538 None:intergenic 30.0%
ATCTAAACTGTCCATTTGTT+CGG - Chr8:89588962-89588981 None:intergenic 30.0%
GAGAATAGAACGATTACAAA+GGG - Chr8:89589520-89589539 None:intergenic 30.0%
AGAGAATCAAGAACCATTCT+CGG + Chr8:89588928-89588947 MsG0880047681.01.T01:CDS 35.0%
CAAATGGACAGTTTAGATTC+AGG + Chr8:89588964-89588983 MsG0880047681.01.T01:CDS 35.0%
CAACAACAACAAAACGAAGA+AGG + Chr8:89589099-89589118 MsG0880047681.01.T01:CDS 35.0%
CGAGAATAGAACGATTACAA+AGG - Chr8:89589521-89589540 None:intergenic 35.0%
CGGTTTATTAACCGAACAAA+TGG + Chr8:89588948-89588967 MsG0880047681.01.T01:CDS 35.0%
TTCGGTTAATAAACCGAGAA+TGG - Chr8:89588944-89588963 None:intergenic 35.0%
! AGTTTCGTTGATGAAACTGT+TGG + Chr8:89589323-89589342 MsG0880047681.01.T01:CDS 35.0%
AAAGAACGCTTCAGATTCAC+CGG + Chr8:89589009-89589028 MsG0880047681.01.T01:CDS 40.0%
AAATGAGAGTAATGCTGGTG+CGG + Chr8:89589362-89589381 MsG0880047681.01.T01:CDS 40.0%
CAACAACAAAACGAAGAAGG+TGG + Chr8:89589102-89589121 MsG0880047681.01.T01:CDS 40.0%
GATGAAACTGTTGGAGGAAA+CGG + Chr8:89589332-89589351 MsG0880047681.01.T01:CDS 40.0%
GTGGAAAATGAGAGTAATGC+TGG + Chr8:89589357-89589376 MsG0880047681.01.T01:CDS 40.0%
GTGTGCATGAGAAGAAAGAA+AGG + Chr8:89589417-89589436 MsG0880047681.01.T01:CDS 40.0%
TGATGTTCGTTGCGGATAAT+TGG - Chr8:89589069-89589088 None:intergenic 40.0%
TTCATCAACGAAACTCTCCA+CGG - Chr8:89589318-89589337 None:intergenic 40.0%
TTTCACATTCCGGTTCAACT+GGG - Chr8:89589182-89589201 None:intergenic 40.0%
! GATGATGATGATGTTCGTTG+CGG - Chr8:89589077-89589096 None:intergenic 40.0%
! TTCGTTGATGAAACTGTTGG+AGG + Chr8:89589326-89589345 MsG0880047681.01.T01:CDS 40.0%
! TTTTTGCAGAGTGTGTTCGA+GGG + Chr8:89589467-89589486 MsG0880047681.01.T01:CDS 40.0%
!! TTCTTTGAGACTACGTCTTC+TGG - Chr8:89588995-89589014 None:intergenic 40.0%
GATAATTGGACGGAAAACCC+AGG - Chr8:89589055-89589074 None:intergenic 45.0%
GTTCGTTGCGGATAATTGGA+CGG - Chr8:89589065-89589084 None:intergenic 45.0%
GTTTCACATTCCGGTTCAAC+TGG - Chr8:89589183-89589202 None:intergenic 45.0%
TCAACTCGGTCTCATATTGC+AGG + Chr8:89588888-89588907 MsG0880047681.01.T01:CDS 45.0%
TGAACCGGAATGTGAAACTC+CGG + Chr8:89589185-89589204 MsG0880047681.01.T01:CDS 45.0%
! CTGCAAAAAGTGTGTCCACA+CGG - Chr8:89589456-89589475 None:intergenic 45.0%
! CTTTTTGCAGAGTGTGTTCG+AGG + Chr8:89589466-89589485 MsG0880047681.01.T01:CDS 45.0%
! TTGTTGTTGTTGCTCCACTG+TGG - Chr8:89589130-89589149 None:intergenic 45.0%
! TTTGAGACTACGTCTTCTGG+TGG - Chr8:89588992-89589011 None:intergenic 45.0%
!! TGGTTCTGGAATGAACCTTG+CGG + Chr8:89589221-89589240 MsG0880047681.01.T01:CDS 45.0%
AATTGGACGGAAAACCCAGG+TGG - Chr8:89589052-89589071 None:intergenic 50.0%
ACCACCGGAGTTTCACATTC+CGG - Chr8:89589192-89589211 None:intergenic 50.0%
ACGCTTCAGATTCACCGGTA+TGG + Chr8:89589014-89589033 MsG0880047681.01.T01:CDS 50.0%
ACTGTTGGAGGAAACGGAAG+TGG + Chr8:89589338-89589357 MsG0880047681.01.T01:CDS 50.0%
AGATCCGAACCCAGTTGAAC+CGG + Chr8:89589170-89589189 MsG0880047681.01.T01:CDS 50.0%
AGGGAACTGTGGTTGAATCG+AGG + Chr8:89589486-89589505 MsG0880047681.01.T01:CDS 50.0%
CATTCCGGTTCAACTGGGTT+CGG - Chr8:89589177-89589196 None:intergenic 50.0%
CGCTTCAGATTCACCGGTAT+GGG + Chr8:89589015-89589034 MsG0880047681.01.T01:CDS 50.0%
GAATGTGAAACTCCGGTGGT+GGG + Chr8:89589192-89589211 MsG0880047681.01.T01:CDS 50.0%
! TTCACCGGTATGGGTTGTTG+TGG + Chr8:89589024-89589043 MsG0880047681.01.T01:CDS 50.0%
!! TTCTGGAATGAACCTTGCGG+CGG + Chr8:89589224-89589243 MsG0880047681.01.T01:CDS 50.0%
ACCGGAATGTGAAACTCCGG+TGG + Chr8:89589188-89589207 MsG0880047681.01.T01:CDS 55.0%
GAGTGTGTTCGAGGGAACTG+TGG + Chr8:89589475-89589494 MsG0880047681.01.T01:CDS 55.0%
GGAATGTGAAACTCCGGTGG+TGG + Chr8:89589191-89589210 MsG0880047681.01.T01:CDS 55.0%
GGAGCGGCAAATGAGGGTAA+CGG + Chr8:89589257-89589276 MsG0880047681.01.T01:CDS 55.0%
GGCACCACAACAACCCATAC+CGG - Chr8:89589031-89589050 None:intergenic 55.0%
TAATGCTGGTGCGGTGGTTG+TGG + Chr8:89589371-89589390 MsG0880047681.01.T01:CDS 55.0%
TGAGAGTAATGCTGGTGCGG+TGG + Chr8:89589365-89589384 MsG0880047681.01.T01:CDS 55.0%
!! GCAAATGAGGGTAACGGAGG+AGG + Chr8:89589263-89589282 MsG0880047681.01.T01:CDS 55.0%
AATGAACCTTGCGGCGGCGT+TGG + Chr8:89589230-89589249 MsG0880047681.01.T01:CDS 60.0%
CGAAGAAGGTGGAGCCACAG+TGG + Chr8:89589113-89589132 MsG0880047681.01.T01:CDS 60.0%
CTGGTGCGGTGGTTGTGGAA+GGG + Chr8:89589376-89589395 MsG0880047681.01.T01:CDS 60.0%
GAACCAGACGAACCCACCAC+CGG - Chr8:89589207-89589226 None:intergenic 60.0%
GGTGCAGCGTTCATACCGTG+TGG + Chr8:89589438-89589457 MsG0880047681.01.T01:CDS 60.0%
! GCGGCAAATGAGGGTAACGG+AGG + Chr8:89589260-89589279 MsG0880047681.01.T01:CDS 60.0%
! GTAACGGAGGAGGCTAGTGG+TGG + Chr8:89589273-89589292 MsG0880047681.01.T01:CDS 60.0%
!! AGGGTAACGGAGGAGGCTAG+TGG + Chr8:89589270-89589289 MsG0880047681.01.T01:CDS 60.0%
!! GGGTTGTTGTGGTGCCACCT+GGG + Chr8:89589035-89589054 MsG0880047681.01.T01:CDS 60.0%
!! GTGGTGGGTTCGTCTGGTTC+TGG + Chr8:89589207-89589226 MsG0880047681.01.T01:CDS 60.0%
!! TGGGTTGTTGTGGTGCCACC+TGG + Chr8:89589034-89589053 MsG0880047681.01.T01:CDS 60.0%
ACGGAGGAGGCTAGTGGTGG+TGG + Chr8:89589276-89589295 MsG0880047681.01.T01:CDS 65.0%
GCTGGTGCGGTGGTTGTGGA+AGG + Chr8:89589375-89589394 MsG0880047681.01.T01:CDS 65.0%
!! ACTCCGGTGGTGGGTTCGTC+TGG + Chr8:89589201-89589220 MsG0880047681.01.T01:CDS 65.0%
!! GAGGAGGCTAGTGGTGGTGG+CGG + Chr8:89589279-89589298 MsG0880047681.01.T01:CDS 65.0%
!! GCTAGTGGTGGTGGCGGAGA+AGG + Chr8:89589285-89589304 MsG0880047681.01.T01:CDS 65.0%
GGCGGCGGAGCGGCAAATGA+GGG + Chr8:89589251-89589270 MsG0880047681.01.T01:CDS 70.0%
TGGCGGCGGAGCGGCAAATG+AGG + Chr8:89589250-89589269 MsG0880047681.01.T01:CDS 70.0%
! GAACCTTGCGGCGGCGTTGG+CGG + Chr8:89589233-89589252 MsG0880047681.01.T01:CDS 70.0%
GCGGAGAAGGAGCGACGCCG+TGG + Chr8:89589298-89589317 MsG0880047681.01.T01:CDS 75.0%
!!! CCTTGCGGCGGCGTTGGCGG+CGG + Chr8:89589236-89589255 MsG0880047681.01.T01:CDS 80.0%
! CCGCCGCCAACGCCGCCGCA+AGG - Chr8:89589239-89589258 None:intergenic 85.0%
!!! CGGCGGCGTTGGCGGCGGAG+CGG + Chr8:89589241-89589260 MsG0880047681.01.T01:CDS 85.0%
Chromosome Type Strat End Strand Name
Chr8 gene 89588883 89589560 89588883 ID=MsG0880047681.01;Name=MsG0880047681.01
Chr8 mRNA 89588883 89589560 89588883 ID=MsG0880047681.01.T01;Parent=MsG0880047681.01;Name=MsG0880047681.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|225
Chr8 exon 89588883 89589560 89588883 ID=MsG0880047681.01.T01:exon:18171;Parent=MsG0880047681.01.T01
Chr8 CDS 89588883 89589560 89588883 ID=MsG0880047681.01.T01:cds;Parent=MsG0880047681.01.T01
Gene Sequence

>MsG0880047681.01.T01

ATGAGTCAACTCGGTCTCATATTGCAGGAATCTCTGCGTTCTCAGAGAGAATCAAGAACCATTCTCGGTTTATTAACCGAACAAATGGACAGTTTAGATTCAGGAACCACCAGAAGACGTAGTCTCAAAGAACGCTTCAGATTCACCGGTATGGGTTGTTGTGGTGCCACCTGGGTTTTCCGTCCAATTATCCGCAACGAACATCATCATCATCAACAACAACAACAAAACGAAGAAGGTGGAGCCACAGTGGAGCAACAACAACAACAGCTACAAGTTCAAGATCAAGATCCGAACCCAGTTGAACCGGAATGTGAAACTCCGGTGGTGGGTTCGTCTGGTTCTGGAATGAACCTTGCGGCGGCGTTGGCGGCGGAGCGGCAAATGAGGGTAACGGAGGAGGCTAGTGGTGGTGGCGGAGAAGGAGCGACGCCGTGGAGAGTTTCGTTGATGAAACTGTTGGAGGAAACGGAAGTGGAAAATGAGAGTAATGCTGGTGCGGTGGTTGTGGAAGGGAGTGATTCAGTGTGCTGCGTGTGCATGAGAAGAAAGAAAGGTGCAGCGTTCATACCGTGTGGACACACTTTTTGCAGAGTGTGTTCGAGGGAACTGTGGTTGAATCGAGGAAATTGTCCCCTTTGTAATCGTTCTATTCTCGAGATTCTCGACATTTTCTGA

Protein sequence

>MsG0880047681.01.T01

MSQLGLILQESLRSQRESRTILGLLTEQMDSLDSGTTRRRSLKERFRFTGMGCCGATWVFRPIIRNEHHHHQQQQQNEEGGATVEQQQQQLQVQDQDPNPVEPECETPVVGSSGSGMNLAAALAAERQMRVTEEASGGGGEGATPWRVSLMKLLEETEVENESNAGAVVVEGSDSVCCVCMRRKKGAAFIPCGHTFCRVCSRELWLNRGNCPLCNRSILEILDIF*