AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040554.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040554.01.T01 MTR_7g092570 95.370 108 4 1 1 108 1 107 6.01e-58 179
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040554.01.T01 AT3G57860 42.975 121 50 5 1 108 1 115 8.35e-13 62.8

Find 40 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCGGCGGTCTCGTGCTTC+CGG 0.391950 7:-79144667 None:intergenic
GCTCGTGCGCGTCGTTCTCC+AGG 0.444790 7:+79144841 MsG0780040554.01.T01:CDS
TGCGCGTCGTTCTCCAGGTT+CGG 0.449151 7:+79144846 MsG0780040554.01.T01:CDS
ATATCCTGCAACGGCGTCCT+CGG 0.451746 7:-79144949 None:intergenic
GTACCCGAGGACGCCGTTGC+AGG 0.452614 7:+79144945 MsG0780040554.01.T01:CDS
CGATCTGAACCGGCGGTGAA+TGG 0.459393 7:-79144781 None:intergenic
ACGAGCAACTTCGCGACGTA+CGG 0.459911 7:-79144824 None:intergenic
GCGGTGAATGGAGCGGGTGC+AGG 0.472928 7:-79144769 None:intergenic
TCTTGAATCACGGCGGAGAA+AGG 0.474672 7:-79144724 None:intergenic
AGTGAAGAACCGATCTGAAC+CGG 0.482402 7:-79144791 None:intergenic
TATCCTGCAACGGCGTCCTC+GGG 0.507871 7:-79144948 None:intergenic
GAACCGGCGGTGAATGGAGC+GGG 0.509648 7:-79144775 None:intergenic
CTTCCACGGCGAGCGGTGGT+CGG 0.514104 7:-79144880 None:intergenic
TCTTCTTCCACGGCGAGCGG+TGG 0.539685 7:-79144884 None:intergenic
CGAGGAGTGTACTTCCGTCT+TGG 0.543666 7:+79144923 MsG0780040554.01.T01:CDS
ACCGCTCGCCGTGGAAGAAG+AGG 0.550727 7:+79144886 MsG0780040554.01.T01:CDS
CGAGCAACTTCGCGACGTAC+GGG 0.553693 7:-79144823 None:intergenic
GCCTCTTCTTCCACGGCGAG+CGG 0.556561 7:-79144887 None:intergenic
CCACGGCGAGCGGTGGTCGG+AGG 0.576704 7:-79144877 None:intergenic
CATTCACCGCCGGTTCAGAT+CGG 0.577297 7:+79144782 MsG0780040554.01.T01:CDS
AATAGAGTATCCACGTCAAG+AGG 0.577745 7:-79144694 None:intergenic
CCGCGTCATCCCTCTTGACG+TGG 0.577863 7:+79144684 MsG0780040554.01.T01:CDS
TGGAGCGGGTGCAGGTGTAA+CGG 0.585593 7:-79144761 None:intergenic
CCTCCGACCACCGCTCGCCG+TGG 0.590863 7:+79144877 MsG0780040554.01.T01:CDS
CGGCTCAGATTCTTGAATCA+CGG 0.600207 7:-79144734 None:intergenic
TGAACCGGCGGTGAATGGAG+CGG 0.620027 7:-79144776 None:intergenic
GGCGTCCTCGGGTACCAAGA+CGG 0.620398 7:-79144937 None:intergenic
TACTTCCGTCTTGGTACCCG+AGG 0.623356 7:+79144932 MsG0780040554.01.T01:CDS
GGTGCAGGTGTAACGGAGAG+CGG 0.629682 7:-79144754 None:intergenic
GCACCCGCTCCATTCACCGC+CGG 0.637803 7:+79144772 MsG0780040554.01.T01:CDS
CCACGTCAAGAGGGATGACG+CGG 0.645758 7:-79144684 None:intergenic
CACTCCTCGATGCAGTAGCA+CGG 0.657446 7:-79144909 None:intergenic
ATAGAGTATCCACGTCAAGA+GGG 0.662352 7:-79144693 None:intergenic
TAGCACGGCCTCTTCTTCCA+CGG 0.680838 7:-79144894 None:intergenic
TCACGGCGGAGAAAGGTCGT+CGG 0.682516 7:-79144717 None:intergenic
ATAGCAGTGATATCCTGCAA+CGG 0.687557 7:-79144958 None:intergenic
GAAGAACCGATCTGAACCGG+CGG 0.705189 7:-79144788 None:intergenic
CGTCAAGAGGGATGACGCGG+CGG 0.719322 7:-79144681 None:intergenic
CTCAGATTCTTGAATCACGG+CGG 0.734730 7:-79144731 None:intergenic
GAGGCCGTGCTACTGCATCG+AGG 0.762826 7:+79144905 MsG0780040554.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAGCAGTGATATCCTGCAA+CGG - Chr7:79144961-79144980 None:intergenic 40.0%
! AATAGAGTATCCACGTCAAG+AGG - Chr7:79144697-79144716 None:intergenic 40.0%
! ATAGAGTATCCACGTCAAGA+GGG - Chr7:79144696-79144715 None:intergenic 40.0%
AGTGAAGAACCGATCTGAAC+CGG - Chr7:79144794-79144813 None:intergenic 45.0%
CGGCTCAGATTCTTGAATCA+CGG - Chr7:79144737-79144756 None:intergenic 45.0%
CTCAGATTCTTGAATCACGG+CGG - Chr7:79144734-79144753 None:intergenic 45.0%
TCTTGAATCACGGCGGAGAA+AGG - Chr7:79144727-79144746 None:intergenic 50.0%
!!! GGAGGAGTATTTTCCGAACC+TGG - Chr7:79144862-79144881 None:intergenic 50.0%
ACGAGCAACTTCGCGACGTA+CGG - Chr7:79144827-79144846 None:intergenic 55.0%
ATATCCTGCAACGGCGTCCT+CGG - Chr7:79144952-79144971 None:intergenic 55.0%
CACTCCTCGATGCAGTAGCA+CGG - Chr7:79144912-79144931 None:intergenic 55.0%
GAAGAACCGATCTGAACCGG+CGG - Chr7:79144791-79144810 None:intergenic 55.0%
TACTTCCGTCTTGGTACCCG+AGG + Chr7:79144932-79144951 MsG0780040554.01.T01:CDS 55.0%
TAGCACGGCCTCTTCTTCCA+CGG - Chr7:79144897-79144916 None:intergenic 55.0%
! CGAGGAGTGTACTTCCGTCT+TGG + Chr7:79144923-79144942 MsG0780040554.01.T01:CDS 55.0%
!! CATTCACCGCCGGTTCAGAT+CGG + Chr7:79144782-79144801 MsG0780040554.01.T01:CDS 55.0%
CCACGTCAAGAGGGATGACG+CGG - Chr7:79144687-79144706 None:intergenic 60.0%
CGAGCAACTTCGCGACGTAC+GGG - Chr7:79144826-79144845 None:intergenic 60.0%
CGATCTGAACCGGCGGTGAA+TGG - Chr7:79144784-79144803 None:intergenic 60.0%
TATCCTGCAACGGCGTCCTC+GGG - Chr7:79144951-79144970 None:intergenic 60.0%
TCACGGCGGAGAAAGGTCGT+CGG - Chr7:79144720-79144739 None:intergenic 60.0%
TGAACCGGCGGTGAATGGAG+CGG - Chr7:79144779-79144798 None:intergenic 60.0%
TGCGCGTCGTTCTCCAGGTT+CGG + Chr7:79144846-79144865 MsG0780040554.01.T01:CDS 60.0%
TGGAGCGGGTGCAGGTGTAA+CGG - Chr7:79144764-79144783 None:intergenic 60.0%
! GGTGCAGGTGTAACGGAGAG+CGG - Chr7:79144757-79144776 None:intergenic 60.0%
ACCGCTCGCCGTGGAAGAAG+AGG + Chr7:79144886-79144905 MsG0780040554.01.T01:CDS 65.0%
CCGCGTCATCCCTCTTGACG+TGG + Chr7:79144684-79144703 MsG0780040554.01.T01:CDS 65.0%
CGTCAAGAGGGATGACGCGG+CGG - Chr7:79144684-79144703 None:intergenic 65.0%
GAACCGGCGGTGAATGGAGC+GGG - Chr7:79144778-79144797 None:intergenic 65.0%
GCCTCTTCTTCCACGGCGAG+CGG - Chr7:79144890-79144909 None:intergenic 65.0%
GGCGTCCTCGGGTACCAAGA+CGG - Chr7:79144940-79144959 None:intergenic 65.0%
TCTTCTTCCACGGCGAGCGG+TGG - Chr7:79144887-79144906 None:intergenic 65.0%
! GAGGCCGTGCTACTGCATCG+AGG + Chr7:79144905-79144924 MsG0780040554.01.T01:CDS 65.0%
ACGCGGCGGTCTCGTGCTTC+CGG - Chr7:79144670-79144689 None:intergenic 70.0%
CTTCCACGGCGAGCGGTGGT+CGG - Chr7:79144883-79144902 None:intergenic 70.0%
GCACCCGCTCCATTCACCGC+CGG + Chr7:79144772-79144791 MsG0780040554.01.T01:CDS 70.0%
GCGGTGAATGGAGCGGGTGC+AGG - Chr7:79144772-79144791 None:intergenic 70.0%
GCTCGTGCGCGTCGTTCTCC+AGG + Chr7:79144841-79144860 MsG0780040554.01.T01:CDS 70.0%
GTACCCGAGGACGCCGTTGC+AGG + Chr7:79144945-79144964 MsG0780040554.01.T01:CDS 70.0%
! CCACGGCGAGCGGTGGTCGG+AGG - Chr7:79144880-79144899 None:intergenic 80.0%
! CCTCCGACCACCGCTCGCCG+TGG + Chr7:79144877-79144896 MsG0780040554.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79144664 79144999 79144664 ID=MsG0780040554.01;Name=MsG0780040554.01
Chr7 mRNA 79144664 79144999 79144664 ID=MsG0780040554.01.T01;Parent=MsG0780040554.01;Name=MsG0780040554.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|111
Chr7 exon 79144664 79144999 79144664 ID=MsG0780040554.01.T01:exon:2891;Parent=MsG0780040554.01.T01
Chr7 CDS 79144664 79144999 79144664 ID=MsG0780040554.01.T01:cds;Parent=MsG0780040554.01.T01
Gene Sequence

>MsG0780040554.01.T01

ATGCCGGAAGCACGAGACCGCCGCGTCATCCCTCTTGACGTGGATACTCTATTCCGACGACCTTTCTCCGCCGTGATTCAAGAATCTGAGCCGCTCTCCGTTACACCTGCACCCGCTCCATTCACCGCCGGTTCAGATCGGTTCTTCACTGAAAGAACTCCCGTACGTCGCGAAGTTGCTCGTGCGCGTCGTTCTCCAGGTTCGGAAAATACTCCTCCGACCACCGCTCGCCGTGGAAGAAGAGGCCGTGCTACTGCATCGAGGAGTGTACTTCCGTCTTGGTACCCGAGGACGCCGTTGCAGGATATCACTGCTATTGTTCGAGTAAATCTCTGA

Protein sequence

>MsG0780040554.01.T01

MPEARDRRVIPLDVDTLFRRPFSAVIQESEPLSVTPAPAPFTAGSDRFFTERTPVRREVARARRSPGSENTPPTTARRGRRGRATASRSVLPSWYPRTPLQDITAIVRVNL*