AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0115540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115540 MtrunA17_Chr1g0212361 93.913 115 7 0 1 115 629 743 1.49e-71 227
Msa0115540 MtrunA17_Chr5g0420581 39.506 81 48 1 1 80 346 426 1.40e-15 71.2
Msa0115540 MtrunA17_Chr8g0374081 38.947 95 56 2 3 96 655 748 8.14e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0115540 AT1G50380.1 83.478 115 19 0 1 115 571 685 4.54e-65 209
Msa0115540 AT1G50380.2 83.478 115 19 0 1 115 637 751 1.17e-64 209
Msa0115540 AT1G69020.2 38.983 118 69 2 1 115 648 765 2.15e-20 85.5
Msa0115540 AT1G69020.1 38.983 118 69 2 1 115 616 733 2.27e-20 85.5
Msa0115540 AT5G66960.1 41.053 95 54 2 3 96 661 754 8.32e-14 66.6

Find 64 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTACAGCTGGATTAAA+TGG 0.255776 1_3:+34388047 Msa0115540:CDS
TATGTTCCCTCTGTGGTATA+TGG 0.264827 1_3:+34385402 None:intergenic
TGCATAAATGGCAGAAGTGC+TGG 0.347256 1_3:+34385782 Msa0115540:five_prime_UTR
TTATAGAATCAAGAAGAAAT+AGG 0.347944 1_3:-34385525 None:intergenic
AAGCCTTGATGACTTGAAAC+TGG 0.366111 1_3:-34385586 None:intergenic
TACATTATCAACAAGGGAAT+AGG 0.376538 1_3:-34387848 None:intergenic
TGCTGTTGCCAGAGTACCTT+TGG 0.379745 1_3:+34385856 Msa0115540:CDS
ATGTTCCCTCTGTGGTATAT+GGG 0.396671 1_3:+34385403 None:intergenic
TGTTGTTTAAATGTGAGCTT+GGG 0.399931 1_3:+34388303 Msa0115540:CDS
TTTAAATGTGAGCTTGGGGC+TGG 0.402664 1_3:+34388308 Msa0115540:CDS
ATACGCGGAGGTGGTGAAAT+GGG 0.407509 1_3:+34385648 Msa0115540:five_prime_UTR
GGGAGGCAGTGGTATGAGAA+TGG 0.419208 1_3:+34385669 Msa0115540:five_prime_UTR
CCACATATTCTTGTTACAGC+TGG 0.425203 1_3:+34388038 Msa0115540:CDS
ATTTATGCAAAATCTTTCCT+TGG 0.433872 1_3:-34385768 None:intergenic
TATACGCGGAGGTGGTGAAA+TGG 0.448082 1_3:+34385647 Msa0115540:five_prime_UTR
GTGGTGAAATGGGGAGGCAG+TGG 0.448275 1_3:+34385658 Msa0115540:five_prime_UTR
GAAGTAGTGAGAGGAATAGT+TGG 0.463287 1_3:-34385905 None:intergenic
GCATACCTGTGGACAATTTG+AGG 0.469433 1_3:-34385556 None:intergenic
AAGTAGAATTCCTCCTTCCT+AGG 0.474833 1_3:-34387814 None:intergenic
ACTTCTGCAACCAAAGGAAC+GGG 0.476937 1_3:-34385443 None:intergenic
CCAGCTGTAACAAGAATATG+TGG 0.480307 1_3:-34388038 None:intergenic
GCATAAATGGCAGAAGTGCT+GGG 0.480811 1_3:+34385783 Msa0115540:five_prime_UTR
CAAAGTTCGTGGCAAAATTG+AGG 0.482652 1_3:+34388255 Msa0115540:CDS
CTGTTGTTTAAATGTGAGCT+TGG 0.484448 1_3:+34388302 Msa0115540:CDS
TGTGGACAATTTGAGGTCAC+TGG 0.495237 1_3:-34385549 None:intergenic
TTCTGAACCTGCAAAGTTCG+TGG 0.496672 1_3:+34388244 Msa0115540:CDS
GGAGGCAGTGGTATGAGAAT+GGG 0.502337 1_3:+34385670 Msa0115540:five_prime_UTR
TGTAGGAATGGGGTGATCCT+AGG 0.505042 1_3:+34387797 Msa0115540:intron
AAAGTTCGTGGCAAAATTGA+GGG 0.507662 1_3:+34388256 Msa0115540:CDS
CTCCCAGTTTCAAGTCATCA+AGG 0.514009 1_3:+34385583 Msa0115540:five_prime_UTR
TTCCTCTCACTACTTCCGAC+TGG 0.517796 1_3:+34385912 Msa0115540:CDS
AGAAATAGGATACATGTTCA+AGG 0.523068 1_3:-34385511 None:intergenic
AACATCCACCAAAGGTACTC+TGG 0.534806 1_3:-34385864 None:intergenic
AACTTCTGCAACCAAAGGAA+CGG 0.537617 1_3:-34385444 None:intergenic
TGTTGCCAGAGTACCTTTGG+TGG 0.562757 1_3:+34385859 Msa0115540:CDS
TCCTCTCACTACTTCCGACT+GGG 0.571133 1_3:+34385913 Msa0115540:CDS
CATAAATGGCAGAAGTGCTG+GGG 0.572685 1_3:+34385784 Msa0115540:five_prime_UTR
TGTTCTTACATTATCAACAA+GGG 0.574837 1_3:-34387854 None:intergenic
TGCAAAACTTCTGCAACCAA+AGG 0.576058 1_3:-34385449 None:intergenic
ATATATTTATGTTCCCTCTG+TGG 0.582115 1_3:+34385395 None:intergenic
ATAGCTCATATACGCGGAGG+TGG 0.584461 1_3:+34385639 Msa0115540:five_prime_UTR
TTGTTCTTACATTATCAACA+AGG 0.588588 1_3:-34387855 None:intergenic
AGGCTTTCATTGTTAGATCG+AGG 0.595178 1_3:+34385603 Msa0115540:five_prime_UTR
GGAATGGGGTGATCCTAGGA+AGG 0.598189 1_3:+34387801 Msa0115540:intron
CTTATGTTACTGTAGGAATG+GGG 0.605360 1_3:+34387787 Msa0115540:intron
TACGCGGAGGTGGTGAAATG+GGG 0.610390 1_3:+34385649 Msa0115540:five_prime_UTR
AGTGACCTCAAATTGTCCAC+AGG 0.623979 1_3:+34385551 Msa0115540:five_prime_UTR
AAAGAAATTTACCTCCCAGT+CGG 0.627238 1_3:-34385927 None:intergenic
TAGCGCCCATATACCACAGA+GGG 0.629209 1_3:-34385408 None:intergenic
AGCCTTGATGACTTGAAACT+GGG 0.633541 1_3:-34385585 None:intergenic
GCGGAGGTGGTGAAATGGGG+AGG 0.638605 1_3:+34385652 Msa0115540:five_prime_UTR
CTGGGAGCTATGCATACCTG+TGG 0.652188 1_3:-34385567 None:intergenic
TCCCAGTCGGAAGTAGTGAG+AGG 0.656613 1_3:-34385914 None:intergenic
GTTGTTTAAATGTGAGCTTG+GGG 0.656860 1_3:+34388304 Msa0115540:CDS
GGTTCAGAATACAAAACACG+TGG 0.664416 1_3:-34388230 None:intergenic
CAGCTGTAACAAGAATATGT+GGG 0.675217 1_3:-34388037 None:intergenic
ATGGGGTGATCCTAGGAAGG+AGG 0.677198 1_3:+34387804 Msa0115540:CDS
GTTGTCACAACATCCACCAA+AGG 0.686944 1_3:-34385872 None:intergenic
GTTATAGCTCATATACGCGG+AGG 0.697147 1_3:+34385636 Msa0115540:five_prime_UTR
ATAAATGGCAGAAGTGCTGG+GGG 0.697795 1_3:+34385785 Msa0115540:five_prime_UTR
TCTCACTACTTCCGACTGGG+AGG 0.702049 1_3:+34385916 Msa0115540:CDS
TATGTTATAGCTCATATACG+CGG 0.752567 1_3:+34385633 Msa0115540:five_prime_UTR
GTTCAGAATACAAAACACGT+GGG 0.759019 1_3:-34388229 None:intergenic
ATAGCGCCCATATACCACAG+AGG 0.791712 1_3:-34385409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTATATTAATTTTTT+TGG - chr1_3:34386608-34386627 None:intergenic 0.0%
!!! TGTGTTTTATTTTATATAAA+GGG - chr1_3:34386461-34386480 None:intergenic 10.0%
!!! TTGTGTTTTATTTTATATAA+AGG - chr1_3:34386462-34386481 None:intergenic 10.0%
!!! TTTTTTTTAAGAAATTCTAT+AGG + chr1_3:34386859-34386878 Msa0115540:intron 10.0%
!!! TTTTATATAAAGGGTAAATT+TGG - chr1_3:34386452-34386471 None:intergenic 15.0%
!!! TAATTTTTTTGGAAACAAAT+TGG - chr1_3:34386597-34386616 None:intergenic 15.0%
!! TAAAACATTCTATTATTTAG+TGG + chr1_3:34388121-34388140 Msa0115540:intron 15.0%
!! TTATAGAATCAAGAAGAAAT+AGG - chr1_3:34385528-34385547 None:intergenic 20.0%
!!! TTTGAATCATTTTCATATGT+TGG - chr1_3:34385974-34385993 None:intergenic 20.0%
!!! ATTTTATAACTTGTAAGAGA+AGG + chr1_3:34386239-34386258 Msa0115540:intron 20.0%
!! AAAAAAATAAGGAAGGTTAA+AGG - chr1_3:34386306-34386325 None:intergenic 20.0%
!! AAAATCAATTAAAACTAGAG+AGG - chr1_3:34386350-34386369 None:intergenic 20.0%
!!! GTAATGTTTTAACATTCTAT+TGG + chr1_3:34386827-34386846 Msa0115540:intron 20.0%
!!! TTTTTAAGAAATTCTATAGG+AGG + chr1_3:34386862-34386881 Msa0115540:intron 20.0%
!! TATATAATCTAAGTTTGATC+TGG - chr1_3:34386898-34386917 None:intergenic 20.0%
!! TTACATTTCCGAAAATTATT+CGG - chr1_3:34387031-34387050 None:intergenic 20.0%
!!! TGAGTTTTCCAAAAAAAATT+CGG + chr1_3:34387054-34387073 Msa0115540:intron 20.0%
!!! AAACTTTTCCGAATTTTTTT+TGG - chr1_3:34387065-34387084 None:intergenic 20.0%
!! AAAAATTAGAAATACATACC+TGG + chr1_3:34387096-34387115 Msa0115540:intron 20.0%
!!! TGGAATTTTAAAAGATTTGT+AGG + chr1_3:34387220-34387239 Msa0115540:intron 20.0%
!!! GGAATTTTAAAAGATTTGTA+GGG + chr1_3:34387221-34387240 Msa0115540:intron 20.0%
!!! GAATTTTAAAAGATTTGTAG+GGG + chr1_3:34387222-34387241 Msa0115540:intron 20.0%
!! AAATCATGTTGAAATTGTTT+CGG - chr1_3:34387461-34387480 None:intergenic 20.0%
!!! AATTTGTTTGTCTTTTAAAC+TGG + chr1_3:34387583-34387602 Msa0115540:intron 20.0%
! TTGAAAAAAATTCTGTTCCA+AGG + chr1_3:34385751-34385770 Msa0115540:five_prime_UTR 25.0%
! ATTTATGCAAAATCTTTCCT+TGG - chr1_3:34385771-34385790 None:intergenic 25.0%
!! AAGGAAAGATTTTGCATAAA+TGG + chr1_3:34385770-34385789 Msa0115540:five_prime_UTR 25.0%
!! ATCATTTTCATATGTTGGTA+TGG - chr1_3:34385969-34385988 None:intergenic 25.0%
! TGAGAGAAAAAAAATAAGGA+AGG - chr1_3:34386313-34386332 None:intergenic 25.0%
! GTCATGAGAGAAAAAAAATA+AGG - chr1_3:34386317-34386336 None:intergenic 25.0%
!!! ATTTTTTTCAGAAAATGTGG+GGG - chr1_3:34386497-34386516 None:intergenic 25.0%
!!! CATTTTTTTCAGAAAATGTG+GGG - chr1_3:34386498-34386517 None:intergenic 25.0%
!!! CCATTTTTTTCAGAAAATGT+GGG - chr1_3:34386499-34386518 None:intergenic 25.0%
!!! ACCATTTTTTTCAGAAAATG+TGG - chr1_3:34386500-34386519 None:intergenic 25.0%
! AAGAATAAAGCATGAGATTA+AGG - chr1_3:34386754-34386773 None:intergenic 25.0%
!!! ATGTTTTAACATTCTATTGG+AGG + chr1_3:34386830-34386849 Msa0115540:intron 25.0%
!! AAATTCTATAGGAGGATTTT+AGG + chr1_3:34386870-34386889 Msa0115540:intron 25.0%
! AAATCACAATAAATCCTTTC+CGG - chr1_3:34386972-34386991 None:intergenic 25.0%
! AAAGGATTTATTGTGATTTC+CGG + chr1_3:34386973-34386992 Msa0115540:intron 25.0%
!!! AACATTTCTAGTATACATAC+CGG - chr1_3:34386995-34387014 None:intergenic 25.0%
!!! AATTCGGAAAAGTTTTATAG+TGG + chr1_3:34387070-34387089 Msa0115540:intron 25.0%
! ATAAAAGGTTAAATAGCACA+AGG + chr1_3:34387189-34387208 Msa0115540:intron 25.0%
! TAAAAGGTTAAATAGCACAA+GGG + chr1_3:34387190-34387209 Msa0115540:intron 25.0%
! ACTCGGTTTATTTATCAATT+TGG - chr1_3:34387365-34387384 None:intergenic 25.0%
!! ATATAATTTTGTGCACATAC+TGG + chr1_3:34387400-34387419 Msa0115540:intron 25.0%
! AATCATGTTGAAATTGTTTC+GGG - chr1_3:34387460-34387479 None:intergenic 25.0%
!! ATACTTTTAATGCATCAAGT+TGG - chr1_3:34387508-34387527 None:intergenic 25.0%
! TTTAAACTGGTATTAAGAAG+AGG + chr1_3:34387596-34387615 Msa0115540:intron 25.0%
! TAAAACTCACATACTTATCT+CGG - chr1_3:34387656-34387675 None:intergenic 25.0%
!!! GATTTCTTTTGCTTTTCTTT+TGG + chr1_3:34387748-34387767 Msa0115540:intron 25.0%
! TGTTCTTACATTATCAACAA+GGG - chr1_3:34387857-34387876 None:intergenic 25.0%
! TTGTTCTTACATTATCAACA+AGG - chr1_3:34387858-34387877 None:intergenic 25.0%
! TCAGACAAAAAATAAATAGG+CGG - chr1_3:34387917-34387936 None:intergenic 25.0%
! TGCTCAGACAAAAAATAAAT+AGG - chr1_3:34387920-34387939 None:intergenic 25.0%
!! AATTTTTATTCTCTCCTGTA+AGG + chr1_3:34387941-34387960 Msa0115540:intron 25.0%
! TTTAGTGGTCACTAATAATT+TGG + chr1_3:34388136-34388155 Msa0115540:intron 25.0%
AGAAATAGGATACATGTTCA+AGG - chr1_3:34385514-34385533 None:intergenic 30.0%
TATGTTATAGCTCATATACG+CGG + chr1_3:34385633-34385652 Msa0115540:five_prime_UTR 30.0%
!!! TATGAGACCAGATTTTTTCA+AGG + chr1_3:34385832-34385851 Msa0115540:exon 30.0%
!! TTCTGTTTGATTTTCTCTCA+TGG + chr1_3:34386015-34386034 Msa0115540:intron 30.0%
!! TCTCATGGTGTCTTTTAAAA+TGG + chr1_3:34386030-34386049 Msa0115540:intron 30.0%
!!! CTCATGGTGTCTTTTAAAAT+GGG + chr1_3:34386031-34386050 Msa0115540:intron 30.0%
AATACAAATGCATGCTGTTA+GGG - chr1_3:34386182-34386201 None:intergenic 30.0%
AAGGTCAATTAAAACTAGAG+AGG - chr1_3:34386287-34386306 None:intergenic 30.0%
!!! TTTTTTTTCTCTCATGACAC+TGG + chr1_3:34386319-34386338 Msa0115540:intron 30.0%
CAATTAAAACTAGAGAGGAT+GGG - chr1_3:34386345-34386364 None:intergenic 30.0%
TCAATTAAAACTAGAGAGGA+TGG - chr1_3:34386346-34386365 None:intergenic 30.0%
GAAGTTTATGTACAATGTTG+GGG - chr1_3:34386430-34386449 None:intergenic 30.0%
GGAAGTTTATGTACAATGTT+GGG - chr1_3:34386431-34386450 None:intergenic 30.0%
TGGAAGTTTATGTACAATGT+TGG - chr1_3:34386432-34386451 None:intergenic 30.0%
!! CCCACATTTTCTGAAAAAAA+TGG + chr1_3:34386496-34386515 Msa0115540:intron 30.0%
AAGAAAGCTTACTGTTTGTT+AGG - chr1_3:34386547-34386566 None:intergenic 30.0%
ATTTCACGAAAATCTTGAAC+CGG + chr1_3:34386950-34386969 Msa0115540:intron 30.0%
AAAAGGTTAAATAGCACAAG+GGG + chr1_3:34387191-34387210 Msa0115540:intron 30.0%
ACCAAAAAAAAAACATGCCA+GGG - chr1_3:34387300-34387319 None:intergenic 30.0%
TACCAAAAAAAAAACATGCC+AGG - chr1_3:34387301-34387320 None:intergenic 30.0%
!!! TTTTTTTGGTAAAACATCCC+TGG + chr1_3:34387310-34387329 Msa0115540:intron 30.0%
CATTTGCTTGTTTACTTTCA+TGG - chr1_3:34387536-34387555 None:intergenic 30.0%
AAGTAAACAAGCAAATGTCT+CGG + chr1_3:34387539-34387558 Msa0115540:intron 30.0%
TGATTTCCTTATGTTACTGT+AGG + chr1_3:34387780-34387799 Msa0115540:intron 30.0%
TACATTATCAACAAGGGAAT+AGG - chr1_3:34387851-34387870 None:intergenic 30.0%
AGAACAAATACTGCACTTAA+TGG + chr1_3:34387871-34387890 Msa0115540:intron 30.0%
AAAAAATAAATAGGCGGTGA+AGG - chr1_3:34387911-34387930 None:intergenic 30.0%
! TGTTGTTTAAATGTGAGCTT+GGG + chr1_3:34388303-34388322 Msa0115540:CDS 30.0%
AAAGAAATTTACCTCCCAGT+CGG - chr1_3:34385930-34385949 None:intergenic 35.0%
TTTCATATGTTGGTATGGAG+TGG - chr1_3:34385964-34385983 None:intergenic 35.0%
! ACGTGTTTTATGTTGCCTTT+AGG + chr1_3:34386080-34386099 Msa0115540:intron 35.0%
! CGTTTTCTTATGCTTCCTAA+AGG - chr1_3:34386098-34386117 None:intergenic 35.0%
ATACAAATGCATGCTGTTAG+GGG - chr1_3:34386181-34386200 None:intergenic 35.0%
CAATACAAATGCATGCTGTT+AGG - chr1_3:34386183-34386202 None:intergenic 35.0%
TTCCGTTTAGAAGAAATCAC+AGG + chr1_3:34386368-34386387 Msa0115540:intron 35.0%
TCCGTTTAGAAGAAATCACA+GGG + chr1_3:34386369-34386388 Msa0115540:intron 35.0%
TCCCTGTGATTTCTTCTAAA+CGG - chr1_3:34386373-34386392 None:intergenic 35.0%
! TTTCTGAAAAAAATGGTCGC+TGG + chr1_3:34386503-34386522 Msa0115540:intron 35.0%
! TTCTGAAAAAAATGGTCGCT+GGG + chr1_3:34386504-34386523 Msa0115540:intron 35.0%
CGTTGAAAAGAATACGATCA+TGG - chr1_3:34386674-34386693 None:intergenic 35.0%
! TGATCGTATTCTTTTCAACG+AGG + chr1_3:34386674-34386693 Msa0115540:intron 35.0%
! CGAGTCTTCCGAATAATTTT+CGG + chr1_3:34387020-34387039 Msa0115540:intron 35.0%
ATACCTGGAAGAATATCATG+TGG + chr1_3:34387111-34387130 Msa0115540:intron 35.0%
TACCTGGAAGAATATCATGT+GGG + chr1_3:34387112-34387131 Msa0115540:intron 35.0%
AATAGCACAAGGGGTAAAAT+TGG + chr1_3:34387200-34387219 Msa0115540:intron 35.0%
!!! TCCCTGGCATGTTTTTTTTT+TGG + chr1_3:34387296-34387315 Msa0115540:intron 35.0%
AATTATATGACACAGCAGCA+AGG - chr1_3:34387388-34387407 None:intergenic 35.0%
!! ATTTTGTGCACATACTGGTT+TGG + chr1_3:34387405-34387424 Msa0115540:intron 35.0%
TTGTTTCGGGAAATGAGAAA+TGG - chr1_3:34387447-34387466 None:intergenic 35.0%
AGCTACTATTTGTAGCTTCA+TGG - chr1_3:34387484-34387503 None:intergenic 35.0%
TATCTCGGAAGGAATATAAG+CGG - chr1_3:34387641-34387660 None:intergenic 35.0%
TCATTCCATACGATATCATG+TGG - chr1_3:34387730-34387749 None:intergenic 35.0%
TCCTTATGTTACTGTAGGAA+TGG + chr1_3:34387785-34387804 Msa0115540:intron 35.0%
CCTTATGTTACTGTAGGAAT+GGG + chr1_3:34387786-34387805 Msa0115540:intron 35.0%
CTTATGTTACTGTAGGAATG+GGG + chr1_3:34387787-34387806 Msa0115540:intron 35.0%
TTATTCTCTCCTGTAAGGAA+TGG + chr1_3:34387946-34387965 Msa0115540:intron 35.0%
! TTCTATTTCTCGTTCTATGC+AGG + chr1_3:34387998-34388017 Msa0115540:intron 35.0%
CAGCTGTAACAAGAATATGT+GGG - chr1_3:34388040-34388059 None:intergenic 35.0%
CTTGTTACAGCTGGATTAAA+TGG + chr1_3:34388047-34388066 Msa0115540:CDS 35.0%
GTGGTCACTAATAATTTGGT+TGG + chr1_3:34388140-34388159 Msa0115540:intron 35.0%
GTTCAGAATACAAAACACGT+GGG - chr1_3:34388232-34388251 None:intergenic 35.0%
AAAGTTCGTGGCAAAATTGA+GGG + chr1_3:34388256-34388275 Msa0115540:CDS 35.0%
! CTGTTGTTTAAATGTGAGCT+TGG + chr1_3:34388302-34388321 Msa0115540:CDS 35.0%
GTTGTTTAAATGTGAGCTTG+GGG + chr1_3:34388304-34388323 Msa0115540:CDS 35.0%
AACTTCTGCAACCAAAGGAA+CGG - chr1_3:34385447-34385466 None:intergenic 40.0%
TGCAAAACTTCTGCAACCAA+AGG - chr1_3:34385452-34385471 None:intergenic 40.0%
! TGCAGAAGTTTTGCAGTCTA+AGG + chr1_3:34385457-34385476 Msa0115540:five_prime_UTR 40.0%
! AGCCTTGATGACTTGAAACT+GGG - chr1_3:34385588-34385607 None:intergenic 40.0%
! AAGCCTTGATGACTTGAAAC+TGG - chr1_3:34385589-34385608 None:intergenic 40.0%
AGGCTTTCATTGTTAGATCG+AGG + chr1_3:34385603-34385622 Msa0115540:five_prime_UTR 40.0%
ACAGCAGCCTTGAAAAAATC+TGG - chr1_3:34385842-34385861 None:intergenic 40.0%
GAAGTAGTGAGAGGAATAGT+TGG - chr1_3:34385908-34385927 None:intergenic 40.0%
!! CTGTTAGGGGAGTATTCATT+CGG - chr1_3:34386168-34386187 None:intergenic 40.0%
! TTAAAACTAGAGAGGTGTGC+AGG - chr1_3:34386279-34386298 None:intergenic 40.0%
! GGTATAATAGTAGGGGTGTA+GGG - chr1_3:34386403-34386422 None:intergenic 40.0%
! AGGTATAATAGTAGGGGTGT+AGG - chr1_3:34386404-34386423 None:intergenic 40.0%
GGGTCAAGGTATAATAGTAG+GGG - chr1_3:34386410-34386429 None:intergenic 40.0%
GGGGTCAAGGTATAATAGTA+GGG - chr1_3:34386411-34386430 None:intergenic 40.0%
TGGGGTCAAGGTATAATAGT+AGG - chr1_3:34386412-34386431 None:intergenic 40.0%
TATGTACAATGTTGGGGTCA+AGG - chr1_3:34386424-34386443 None:intergenic 40.0%
! GCTTACTGTTTGTTAGGAGT+GGG - chr1_3:34386541-34386560 None:intergenic 40.0%
! AGCTTACTGTTTGTTAGGAG+TGG - chr1_3:34386542-34386561 None:intergenic 40.0%
TTCTTCTTCAACGAGAGAAG+TGG - chr1_3:34386726-34386745 None:intergenic 40.0%
ACGAAAATCTTGAACCGGAA+AGG + chr1_3:34386955-34386974 Msa0115540:intron 40.0%
ACCTGGAAGAATATCATGTG+GGG + chr1_3:34387113-34387132 Msa0115540:intron 40.0%
!! AATACAGAAGTACCATGCCA+GGG - chr1_3:34387330-34387349 None:intergenic 40.0%
!! TAATACAGAAGTACCATGCC+AGG - chr1_3:34387331-34387350 None:intergenic 40.0%
!! ACATACTGGTTTGGATGTGT+TGG + chr1_3:34387414-34387433 Msa0115540:intron 40.0%
ACTCACATACTTATCTCGGA+AGG - chr1_3:34387652-34387671 None:intergenic 40.0%
CAACTCCACATGATATCGTA+TGG + chr1_3:34387722-34387741 Msa0115540:intron 40.0%
CCCATTCCTACAGTAACATA+AGG - chr1_3:34387789-34387808 None:intergenic 40.0%
AAGTAGAATTCCTCCTTCCT+AGG - chr1_3:34387817-34387836 None:intergenic 40.0%
TCCTGTAAGGAATGGTTACT+AGG + chr1_3:34387954-34387973 Msa0115540:intron 40.0%
TCCTAGTAACCATTCCTTAC+AGG - chr1_3:34387958-34387977 None:intergenic 40.0%
CCAGCTGTAACAAGAATATG+TGG - chr1_3:34388041-34388060 None:intergenic 40.0%
CCACATATTCTTGTTACAGC+TGG + chr1_3:34388038-34388057 Msa0115540:CDS 40.0%
TGGTAAGCATACCTGATTCT+TGG + chr1_3:34388067-34388086 Msa0115540:intron 40.0%
ATAGTTCAGTGCCAAGAATC+AGG - chr1_3:34388081-34388100 None:intergenic 40.0%
GGTTCAGAATACAAAACACG+TGG - chr1_3:34388233-34388252 None:intergenic 40.0%
! AATTTTGCCACGAACTTTGC+AGG - chr1_3:34388254-34388273 None:intergenic 40.0%
CAAAGTTCGTGGCAAAATTG+AGG + chr1_3:34388255-34388274 Msa0115540:CDS 40.0%
!!! GACTTTTTTCGAAGTCAGGA+AGG + chr1_3:34388330-34388349 Msa0115540:CDS 40.0%
! TTTTAGTCAGCCCGTTCCTT+TGG + chr1_3:34385433-34385452 Msa0115540:five_prime_UTR 45.0%
ACTTCTGCAACCAAAGGAAC+GGG - chr1_3:34385446-34385465 None:intergenic 45.0%
! TGTGGACAATTTGAGGTCAC+TGG - chr1_3:34385552-34385571 None:intergenic 45.0%
AGTGACCTCAAATTGTCCAC+AGG + chr1_3:34385551-34385570 Msa0115540:five_prime_UTR 45.0%
GCATACCTGTGGACAATTTG+AGG - chr1_3:34385559-34385578 None:intergenic 45.0%
! CTCCCAGTTTCAAGTCATCA+AGG + chr1_3:34385583-34385602 Msa0115540:five_prime_UTR 45.0%
GTTATAGCTCATATACGCGG+AGG + chr1_3:34385636-34385655 Msa0115540:five_prime_UTR 45.0%
! TGCATAAATGGCAGAAGTGC+TGG + chr1_3:34385782-34385801 Msa0115540:five_prime_UTR 45.0%
!! GCATAAATGGCAGAAGTGCT+GGG + chr1_3:34385783-34385802 Msa0115540:five_prime_UTR 45.0%
!! CATAAATGGCAGAAGTGCTG+GGG + chr1_3:34385784-34385803 Msa0115540:five_prime_UTR 45.0%
!! ATAAATGGCAGAAGTGCTGG+GGG + chr1_3:34385785-34385804 Msa0115540:five_prime_UTR 45.0%
!! AACATCCACCAAAGGTACTC+TGG - chr1_3:34385867-34385886 None:intergenic 45.0%
GTTGTCACAACATCCACCAA+AGG - chr1_3:34385875-34385894 None:intergenic 45.0%
GATGTCACTGTGAGATGCAT+TGG + chr1_3:34386132-34386151 Msa0115540:intron 45.0%
CAGAAAATGTGGGGGTGAAA+GGG - chr1_3:34386489-34386508 None:intergenic 45.0%
TCAGAAAATGTGGGGGTGAA+AGG - chr1_3:34386490-34386509 None:intergenic 45.0%
CCTGGAAGAATATCATGTGG+GGG + chr1_3:34387114-34387133 Msa0115540:intron 45.0%
! AGGGGTGAAAGTACATGTGT+CGG + chr1_3:34387240-34387259 Msa0115540:intron 45.0%
AAGTACATGTGTCGGGGTAT+AGG + chr1_3:34387248-34387267 Msa0115540:intron 45.0%
AGTACATGTGTCGGGGTATA+GGG + chr1_3:34387249-34387268 Msa0115540:intron 45.0%
TTGGTAAAACATCCCTGGCA+TGG + chr1_3:34387315-34387334 Msa0115540:intron 45.0%
!! GAGGCACTGCACTTAACATA+TGG + chr1_3:34387615-34387634 Msa0115540:intron 45.0%
ATACTGCACTTAATGGCCTC+TGG + chr1_3:34387878-34387897 Msa0115540:intron 45.0%
AAATAGGCGGTGAAGGTTAG+AGG - chr1_3:34387904-34387923 None:intergenic 45.0%
TTCTCGTTCTATGCAGGTCA+AGG + chr1_3:34388004-34388023 Msa0115540:intron 45.0%
TTCTGAACCTGCAAAGTTCG+TGG + chr1_3:34388244-34388263 Msa0115540:CDS 45.0%
TTTAAATGTGAGCTTGGGGC+TGG + chr1_3:34388308-34388327 Msa0115540:CDS 45.0%
!!! GCTGGACTTTTTTCGAAGTC+AGG + chr1_3:34388326-34388345 Msa0115540:CDS 45.0%
!! ATAAAAAATTAATGTATAAA+AGG + chr1_3:34387174-34387193 Msa0115540:intron 5.0%
TAGCGCCCATATACCACAGA+GGG - chr1_3:34385411-34385430 None:intergenic 50.0%
ATAGCGCCCATATACCACAG+AGG - chr1_3:34385412-34385431 None:intergenic 50.0%
ATAGCTCATATACGCGGAGG+TGG + chr1_3:34385639-34385658 Msa0115540:five_prime_UTR 50.0%
TATACGCGGAGGTGGTGAAA+TGG + chr1_3:34385647-34385666 Msa0115540:five_prime_UTR 50.0%
ATACGCGGAGGTGGTGAAAT+GGG + chr1_3:34385648-34385667 Msa0115540:five_prime_UTR 50.0%
GGAGGCAGTGGTATGAGAAT+GGG + chr1_3:34385670-34385689 Msa0115540:five_prime_UTR 50.0%
!! AGTGCTGGGGGTTTGCTAAT+TGG + chr1_3:34385797-34385816 Msa0115540:five_prime_UTR 50.0%
! TGCTGTTGCCAGAGTACCTT+TGG + chr1_3:34385856-34385875 Msa0115540:CDS 50.0%
! TGTTGCCAGAGTACCTTTGG+TGG + chr1_3:34385859-34385878 Msa0115540:CDS 50.0%
TTCCTCTCACTACTTCCGAC+TGG + chr1_3:34385912-34385931 Msa0115540:CDS 50.0%
TCCTCTCACTACTTCCGACT+GGG + chr1_3:34385913-34385932 Msa0115540:CDS 50.0%
CCCCCACATGATATTCTTCC+AGG - chr1_3:34387117-34387136 None:intergenic 50.0%
! GGGGTGAAAGTACATGTGTC+GGG + chr1_3:34387241-34387260 Msa0115540:intron 50.0%
! GGGTGAAAGTACATGTGTCG+GGG + chr1_3:34387242-34387261 Msa0115540:intron 50.0%
CCAGGGATGTAGCTGTGATT+TGG - chr1_3:34387283-34387302 None:intergenic 50.0%
CCAAATCACAGCTACATCCC+TGG + chr1_3:34387280-34387299 Msa0115540:intron 50.0%
ATGACACAGCAGCAAGGACT+CGG - chr1_3:34387382-34387401 None:intergenic 50.0%
TGTAGGAATGGGGTGATCCT+AGG + chr1_3:34387797-34387816 Msa0115540:intron 50.0%
CTGGGAGCTATGCATACCTG+TGG - chr1_3:34385570-34385589 None:intergenic 55.0%
TACGCGGAGGTGGTGAAATG+GGG + chr1_3:34385649-34385668 Msa0115540:five_prime_UTR 55.0%
GGGAGGCAGTGGTATGAGAA+TGG + chr1_3:34385669-34385688 Msa0115540:five_prime_UTR 55.0%
!! GCTGGGGGTTTGCTAATTGG+TGG + chr1_3:34385800-34385819 Msa0115540:five_prime_UTR 55.0%
TCCCAGTCGGAAGTAGTGAG+AGG - chr1_3:34385917-34385936 None:intergenic 55.0%
TCTCACTACTTCCGACTGGG+AGG + chr1_3:34385916-34385935 Msa0115540:CDS 55.0%
GGAATGGGGTGATCCTAGGA+AGG + chr1_3:34387801-34387820 Msa0115540:intron 55.0%
ATGGGGTGATCCTAGGAAGG+AGG + chr1_3:34387804-34387823 Msa0115540:CDS 55.0%
GTGGTGAAATGGGGAGGCAG+TGG + chr1_3:34385658-34385677 Msa0115540:five_prime_UTR 60.0%
CGGTGAAGGTTAGAGGCCAG+AGG - chr1_3:34387897-34387916 None:intergenic 60.0%
GCGGAGGTGGTGAAATGGGG+AGG + chr1_3:34385652-34385671 Msa0115540:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 34385404 34388371 34385404 ID=Msa0115540;Name=Msa0115540
chr1_3 mRNA 34385404 34388371 34385404 ID=Msa0115540-mRNA-1;Parent=Msa0115540;Name=Msa0115540-mRNA-1;_AED=0.42;_eAED=0.42;_QI=429|1|1|1|1|1|4|16|115
chr1_3 exon 34385404 34385937 34385404 ID=Msa0115540-mRNA-1:exon:10848;Parent=Msa0115540-mRNA-1
chr1_3 exon 34387802 34387867 34387802 ID=Msa0115540-mRNA-1:exon:10849;Parent=Msa0115540-mRNA-1
chr1_3 exon 34388020 34388068 34388020 ID=Msa0115540-mRNA-1:exon:10850;Parent=Msa0115540-mRNA-1
chr1_3 exon 34388228 34388371 34388228 ID=Msa0115540-mRNA-1:exon:10851;Parent=Msa0115540-mRNA-1
chr1_3 five_prime_UTR 34385404 34385832 34385404 ID=Msa0115540-mRNA-1:five_prime_utr;Parent=Msa0115540-mRNA-1
chr1_3 CDS 34385833 34385937 34385833 ID=Msa0115540-mRNA-1:cds;Parent=Msa0115540-mRNA-1
chr1_3 CDS 34387802 34387867 34387802 ID=Msa0115540-mRNA-1:cds;Parent=Msa0115540-mRNA-1
chr1_3 CDS 34388020 34388068 34388020 ID=Msa0115540-mRNA-1:cds;Parent=Msa0115540-mRNA-1
chr1_3 CDS 34388228 34388355 34388228 ID=Msa0115540-mRNA-1:cds;Parent=Msa0115540-mRNA-1
chr1_3 three_prime_UTR 34388356 34388371 34388356 ID=Msa0115540-mRNA-1:three_prime_utr;Parent=Msa0115540-mRNA-1
Gene Sequence

>Msa0115540

ATGAGACCAGATTTTTTCAAGGCTGCTGTTGCCAGAGTACCTTTGGTGGATGTTGTGACAACAATGCTTGATCCAACTATTCCTCTCACTACTTCCGACTGGGAGGAATGGGGTGATCCTAGGAAGGAGGAATTCTACTTCTACATGAAGTCCTATTCCCTTGTTGATAATGTCAAGGCACAGAATTACCCACATATTCTTGTTACAGCTGGATTAAATGACCCACGTGTTTTGTATTCTGAACCTGCAAAGTTCGTGGCAAAATTGAGGGATATGAAAACTGATGATAACATACTGTTGTTTAAATGTGAGCTTGGGGCTGGACTTTTTTCGAAGTCAGGAAGGTAA
Protein sequence

>Msa0115540

MRPDFFKAAVARVPLVDVVTTMLDPTIPLTTSDWEEWGDPRKEEFYFYMKSYSLVDNVKAQNYPHILVTAGLNDPRVLYSEPAKFVAKLRDMKTDDNILLFKCELGAGLFSKSGR*