AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0147750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0147750 MtrunA17_Chr1g0157151 86.395 147 3 3 1 131 1 146 1.67e-79 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0147750 AT1G76060.1 59.732 149 38 5 4 132 8 154 2.45e-47 151

Find 40 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATAAGAATACGATTATT+TGG 0.155569 1_4:-7782727 Msa0147750:three_prime_UTR
CATATATATATAACTTTGTT+AGG 0.281795 1_4:+7782833 None:intergenic
TGACACCGTGGAAGAGCTTC+TGG 0.320024 1_4:-7784985 Msa0147750:CDS
ATTGTCACTCGGTTGCTTAT+CGG 0.353596 1_4:-7784732 Msa0147750:CDS
AGTGCAGCCAGCTGCTTACT+TGG 0.357832 1_4:+7782913 None:intergenic
ACGATGCCAAAGGCGTTCTA+TGG 0.381120 1_4:-7784821 Msa0147750:intron
CAGTGCTTCTCGGCGCGTGC+TGG 0.390621 1_4:+7784890 None:intergenic
GAGGAAAATGGCGAAGCCTT+TGG 0.391236 1_4:-7785070 Msa0147750:CDS
TTGGAAGGACCTGTTCTTCA+AGG 0.425614 1_4:-7782969 Msa0147750:CDS
AAGGATCGTCGCGGTGGCAG+TGG 0.428548 1_4:-7784657 Msa0147750:CDS
TTTGCCTCTCCTTGAAGAAC+AGG 0.430131 1_4:+7782960 None:intergenic
AGAAGCTCTTCCACGGTGTC+AGG 0.435099 1_4:+7784987 None:intergenic
TGTAGAGACTCAGTGCTTCT+CGG 0.445236 1_4:+7784880 None:intergenic
AAGAAGAAGAGTTGCTGAAT+CGG 0.468368 1_4:-7784923 Msa0147750:CDS
TGCTAGGTTTGAGACGGATC+CGG 0.469269 1_4:-7784757 Msa0147750:CDS
GTAAAGAGTTTGAGCTTGCT+AGG 0.476524 1_4:-7784773 Msa0147750:CDS
AGCAACCGAGTGACAATCTC+CGG 0.496816 1_4:+7784738 None:intergenic
TCCACACTGATATCAATGTC+TGG 0.526983 1_4:-7782784 Msa0147750:three_prime_UTR
GATCGTCGCGGTGGCAGTGG+CGG 0.542143 1_4:-7784654 Msa0147750:intron
GAGGCAAACGTCGAGTATTG+TGG 0.548864 1_4:-7782945 Msa0147750:CDS
GTCGAGTATTGTGGAATATG+TGG 0.550947 1_4:-7782936 Msa0147750:CDS
GTTTCAGATTCGAATTCCAA+AGG 0.567422 1_4:+7785054 None:intergenic
TATGTGGCCAAGTAAGCAGC+TGG 0.569045 1_4:-7782920 Msa0147750:CDS
TGAGCTTGCTAGGTTTGAGA+CGG 0.572947 1_4:-7784763 Msa0147750:CDS
CCGTTCCAGAAGCTCTTCCA+CGG 0.587827 1_4:+7784980 None:intergenic
CAGCAAACGGTTGCGCGTGG+CGG 0.590852 1_4:-7785025 Msa0147750:CDS
GTCACTCGGTTGCTTATCGG+TGG 0.599413 1_4:-7784729 Msa0147750:CDS
CCGTGGAAGAGCTTCTGGAA+CGG 0.622869 1_4:-7784980 Msa0147750:CDS
AAACAGCAAACGGTTGCGCG+TGG 0.625582 1_4:-7785028 Msa0147750:CDS
GTTGCTTATCGGTGGACGTG+AGG 0.631369 1_4:-7784721 Msa0147750:CDS
AGGACCTGTTCTTCAAGGAG+AGG 0.646252 1_4:-7782964 Msa0147750:CDS
CAACGCTATTGATAAGCTTG+CGG 0.647873 1_4:-7784691 Msa0147750:CDS
AAGAAGAGTTGCTGAATCGG+CGG 0.650336 1_4:-7784920 Msa0147750:CDS
ATTCAGAAGGATCGTCGCGG+TGG 0.657849 1_4:-7784663 Msa0147750:CDS
GCTTGCGGAACAGATTCAGA+AGG 0.661253 1_4:-7784676 Msa0147750:CDS
CGGATCCGGAGATTGTCACT+CGG 0.686306 1_4:-7784743 Msa0147750:CDS
TCCAGACATTGATATCAGTG+TGG 0.694767 1_4:+7782783 None:intergenic
ACACGACGGTCCTGACACCG+TGG 0.698737 1_4:-7784997 Msa0147750:CDS
GCGTGGCGGTTATTACACGA+CGG 0.720396 1_4:-7785011 Msa0147750:CDS
CAGATTCAGAAGGATCGTCG+CGG 0.756987 1_4:-7784666 Msa0147750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTACATAATAATTTAA+AGG - chr1_4:7783174-7783193 Msa0147750:intron 10.0%
!! TTATTAAATATTAATGAATG+TGG + chr1_4:7783590-7783609 None:intergenic 10.0%
!!! TATTTAATAATAATTTTGCA+AGG - chr1_4:7783600-7783619 Msa0147750:intron 10.0%
!!! ATTTAATAATAATTTTGCAA+GGG - chr1_4:7783601-7783620 Msa0147750:intron 10.0%
!!! ATTATTATTTTAAACAAACA+CGG + chr1_4:7784723-7784742 None:intergenic 10.0%
!!! GATTTTACATAATAATTTAA+AGG - chr1_4:7783174-7783193 Msa0147750:intron 10.0%
!! TTATTAAATATTAATGAATG+TGG + chr1_4:7783590-7783609 None:intergenic 10.0%
!!! TATTTAATAATAATTTTGCA+AGG - chr1_4:7783600-7783619 Msa0147750:intron 10.0%
!!! ATTTAATAATAATTTTGCAA+GGG - chr1_4:7783601-7783620 Msa0147750:intron 10.0%
!!! ATTATTATTTTAAACAAACA+CGG + chr1_4:7784723-7784742 None:intergenic 10.0%
!!! ATAAATGTTGATTTAGTTTA+TGG - chr1_4:7783341-7783360 Msa0147750:intron 15.0%
!!! AAATTTTGTTCTCTTTTTAT+GGG + chr1_4:7784417-7784436 None:intergenic 15.0%
!!! TAAATTTTGTTCTCTTTTTA+TGG + chr1_4:7784418-7784437 None:intergenic 15.0%
!! CATATATATATAACTTTGTT+AGG + chr1_4:7784961-7784980 None:intergenic 15.0%
!! ATTATAAGAATACGATTATT+TGG - chr1_4:7785064-7785083 Msa0147750:CDS 15.0%
!!! ATAAATGTTGATTTAGTTTA+TGG - chr1_4:7783341-7783360 Msa0147750:intron 15.0%
!!! AAATTTTGTTCTCTTTTTAT+GGG + chr1_4:7784417-7784436 None:intergenic 15.0%
!!! TAAATTTTGTTCTCTTTTTA+TGG + chr1_4:7784418-7784437 None:intergenic 15.0%
!! CATATATATATAACTTTGTT+AGG + chr1_4:7784961-7784980 None:intergenic 15.0%
!! ATTATAAGAATACGATTATT+TGG - chr1_4:7785064-7785083 Msa0147750:CDS 15.0%
!!! CAAATTAATCATTTTGTGAA+AGG - chr1_4:7783463-7783482 Msa0147750:intron 20.0%
!! TTTCTTGTAAAACAGTTATT+AGG + chr1_4:7783680-7783699 None:intergenic 20.0%
!!! TTCATTTTTAAACACACATA+TGG + chr1_4:7784162-7784181 None:intergenic 20.0%
!!! TAATATCCTGTTGTTATTTT+AGG + chr1_4:7784208-7784227 None:intergenic 20.0%
!!! CAAATTAATCATTTTGTGAA+AGG - chr1_4:7783463-7783482 Msa0147750:intron 20.0%
!! TTTCTTGTAAAACAGTTATT+AGG + chr1_4:7783680-7783699 None:intergenic 20.0%
!!! TTCATTTTTAAACACACATA+TGG + chr1_4:7784162-7784181 None:intergenic 20.0%
!!! TAATATCCTGTTGTTATTTT+AGG + chr1_4:7784208-7784227 None:intergenic 20.0%
!!! TGGTTTGATTTGATTTGATT+TGG - chr1_4:7783244-7783263 Msa0147750:intron 25.0%
!!! TGATTTGATTTGATTTGGTT+TGG - chr1_4:7783249-7783268 Msa0147750:intron 25.0%
! AAACATAATCCTGAAAATGA+AGG - chr1_4:7783433-7783452 Msa0147750:intron 25.0%
! AGGGAAACTAATAAATTCAT+AGG - chr1_4:7783620-7783639 Msa0147750:intron 25.0%
! TCTAGAGATGAATTAGAAAA+GGG + chr1_4:7783818-7783837 None:intergenic 25.0%
! TTTAAGTATTCAGCAAAAGA+GGG + chr1_4:7783993-7784012 None:intergenic 25.0%
! ATTTAAGTATTCAGCAAAAG+AGG + chr1_4:7783994-7784013 None:intergenic 25.0%
! AAAACTAACTAATGATGACA+CGG + chr1_4:7784069-7784088 None:intergenic 25.0%
! TAAAGTCCTAAAATAACAAC+AGG - chr1_4:7784199-7784218 Msa0147750:intron 25.0%
! ACTACACTTCTCTATATTAA+AGG + chr1_4:7784319-7784338 None:intergenic 25.0%
! GAGCTATTTATATACATGTA+TGG - chr1_4:7784632-7784651 Msa0147750:intron 25.0%
! TTTATATACATGTATGGACT+TGG - chr1_4:7784638-7784657 Msa0147750:intron 25.0%
! TAAGTAAAGAAACTAAATCG+AGG + chr1_4:7784784-7784803 None:intergenic 25.0%
!! ATATTCACACTTTTCAGTTT+TGG - chr1_4:7784803-7784822 Msa0147750:CDS 25.0%
!!! TGGTTTGATTTGATTTGATT+TGG - chr1_4:7783244-7783263 Msa0147750:intron 25.0%
!!! TGATTTGATTTGATTTGGTT+TGG - chr1_4:7783249-7783268 Msa0147750:intron 25.0%
! AAACATAATCCTGAAAATGA+AGG - chr1_4:7783433-7783452 Msa0147750:intron 25.0%
! AGGGAAACTAATAAATTCAT+AGG - chr1_4:7783620-7783639 Msa0147750:intron 25.0%
! TCTAGAGATGAATTAGAAAA+GGG + chr1_4:7783818-7783837 None:intergenic 25.0%
! TTTAAGTATTCAGCAAAAGA+GGG + chr1_4:7783993-7784012 None:intergenic 25.0%
! ATTTAAGTATTCAGCAAAAG+AGG + chr1_4:7783994-7784013 None:intergenic 25.0%
! AAAACTAACTAATGATGACA+CGG + chr1_4:7784069-7784088 None:intergenic 25.0%
! TAAAGTCCTAAAATAACAAC+AGG - chr1_4:7784199-7784218 Msa0147750:intron 25.0%
! ACTACACTTCTCTATATTAA+AGG + chr1_4:7784319-7784338 None:intergenic 25.0%
! GAGCTATTTATATACATGTA+TGG - chr1_4:7784632-7784651 Msa0147750:intron 25.0%
! TTTATATACATGTATGGACT+TGG - chr1_4:7784638-7784657 Msa0147750:intron 25.0%
! TAAGTAAAGAAACTAAATCG+AGG + chr1_4:7784784-7784803 None:intergenic 25.0%
!! ATATTCACACTTTTCAGTTT+TGG - chr1_4:7784803-7784822 Msa0147750:CDS 25.0%
CAGCAATGATTAACAACAAA+AGG + chr1_4:7783222-7783241 None:intergenic 30.0%
TTGTGAGTTGTAGAATTGAA+TGG - chr1_4:7783273-7783292 Msa0147750:intron 30.0%
TTACTTACTACTTAGCTTCA+AGG - chr1_4:7783531-7783550 Msa0147750:intron 30.0%
CAAGTACAACAAGTTATTCA+AGG - chr1_4:7783713-7783732 Msa0147750:intron 30.0%
! TTATCAATTCTGTCCAGTTT+TGG - chr1_4:7783751-7783770 Msa0147750:intron 30.0%
! TATCAATTCTGTCCAGTTTT+GGG - chr1_4:7783752-7783771 Msa0147750:intron 30.0%
!!! TTCTTTTGGAATTCATTTCC+TGG - chr1_4:7783778-7783797 Msa0147750:intron 30.0%
!!! TCTTTTGGAATTCATTTCCT+GGG - chr1_4:7783779-7783798 Msa0147750:intron 30.0%
ATTAGAAAAGGGAACATGTT+AGG + chr1_4:7783807-7783826 None:intergenic 30.0%
! CTCTAGAGATGAATTAGAAA+AGG + chr1_4:7783819-7783838 None:intergenic 30.0%
AATATTGAGAATCTAGTCAC+TGG + chr1_4:7783847-7783866 None:intergenic 30.0%
GATTCTCAATATTCAATGAC+TGG - chr1_4:7783854-7783873 Msa0147750:intron 30.0%
TATTCAATGACTGGATTTCA+AGG - chr1_4:7783863-7783882 Msa0147750:intron 30.0%
TAATAGAAACAAGCTTCCTA+AGG - chr1_4:7784019-7784038 Msa0147750:intron 30.0%
GTATCTAAATCTTCATGTGT+CGG + chr1_4:7784245-7784264 None:intergenic 30.0%
ACACATGAAGATTTAGATAC+AGG - chr1_4:7784245-7784264 Msa0147750:intron 30.0%
ATTGGTCAACTGTTTGTATT+TGG + chr1_4:7784446-7784465 None:intergenic 30.0%
AAATACAAACAGTTGACCAA+TGG - chr1_4:7784445-7784464 Msa0147750:intron 30.0%
CAAATCATCATAAGGAATGT+GGG + chr1_4:7784482-7784501 None:intergenic 30.0%
! ATTATTCATTTTGCAGCTTG+AGG + chr1_4:7784548-7784567 None:intergenic 30.0%
AAGGAACAATGTAAAATTGC+AGG + chr1_4:7784575-7784594 None:intergenic 30.0%
TTTACATTGTTCCTTCTGAT+AGG - chr1_4:7784580-7784599 Msa0147750:intron 30.0%
TTACATTGTTCCTTCTGATA+GGG - chr1_4:7784581-7784600 Msa0147750:intron 30.0%
CTTATTATAACCCTATCAGA+AGG + chr1_4:7784594-7784613 None:intergenic 30.0%
! ACATGCAAATCATGATAATG+TGG - chr1_4:7784661-7784680 Msa0147750:CDS 30.0%
GACAAGTGAAAATTGATTGT+AGG - chr1_4:7784751-7784770 Msa0147750:CDS 30.0%
CAGCAATGATTAACAACAAA+AGG + chr1_4:7783222-7783241 None:intergenic 30.0%
TTGTGAGTTGTAGAATTGAA+TGG - chr1_4:7783273-7783292 Msa0147750:intron 30.0%
TTACTTACTACTTAGCTTCA+AGG - chr1_4:7783531-7783550 Msa0147750:intron 30.0%
CAAGTACAACAAGTTATTCA+AGG - chr1_4:7783713-7783732 Msa0147750:intron 30.0%
! TTATCAATTCTGTCCAGTTT+TGG - chr1_4:7783751-7783770 Msa0147750:intron 30.0%
! TATCAATTCTGTCCAGTTTT+GGG - chr1_4:7783752-7783771 Msa0147750:intron 30.0%
!!! TTCTTTTGGAATTCATTTCC+TGG - chr1_4:7783778-7783797 Msa0147750:intron 30.0%
!!! TCTTTTGGAATTCATTTCCT+GGG - chr1_4:7783779-7783798 Msa0147750:intron 30.0%
ATTAGAAAAGGGAACATGTT+AGG + chr1_4:7783807-7783826 None:intergenic 30.0%
! CTCTAGAGATGAATTAGAAA+AGG + chr1_4:7783819-7783838 None:intergenic 30.0%
AATATTGAGAATCTAGTCAC+TGG + chr1_4:7783847-7783866 None:intergenic 30.0%
GATTCTCAATATTCAATGAC+TGG - chr1_4:7783854-7783873 Msa0147750:intron 30.0%
TATTCAATGACTGGATTTCA+AGG - chr1_4:7783863-7783882 Msa0147750:intron 30.0%
TAATAGAAACAAGCTTCCTA+AGG - chr1_4:7784019-7784038 Msa0147750:intron 30.0%
GTATCTAAATCTTCATGTGT+CGG + chr1_4:7784245-7784264 None:intergenic 30.0%
ACACATGAAGATTTAGATAC+AGG - chr1_4:7784245-7784264 Msa0147750:intron 30.0%
ATTGGTCAACTGTTTGTATT+TGG + chr1_4:7784446-7784465 None:intergenic 30.0%
AAATACAAACAGTTGACCAA+TGG - chr1_4:7784445-7784464 Msa0147750:intron 30.0%
CAAATCATCATAAGGAATGT+GGG + chr1_4:7784482-7784501 None:intergenic 30.0%
! ATTATTCATTTTGCAGCTTG+AGG + chr1_4:7784548-7784567 None:intergenic 30.0%
AAGGAACAATGTAAAATTGC+AGG + chr1_4:7784575-7784594 None:intergenic 30.0%
TTTACATTGTTCCTTCTGAT+AGG - chr1_4:7784580-7784599 Msa0147750:intron 30.0%
TTACATTGTTCCTTCTGATA+GGG - chr1_4:7784581-7784600 Msa0147750:intron 30.0%
CTTATTATAACCCTATCAGA+AGG + chr1_4:7784594-7784613 None:intergenic 30.0%
! ACATGCAAATCATGATAATG+TGG - chr1_4:7784661-7784680 Msa0147750:CDS 30.0%
GACAAGTGAAAATTGATTGT+AGG - chr1_4:7784751-7784770 Msa0147750:CDS 30.0%
GTTTCAGATTCGAATTCCAA+AGG + chr1_4:7782740-7782759 None:intergenic 35.0%
CTGAAACCAAAAACAGCAAA+CGG - chr1_4:7782753-7782772 Msa0147750:three_prime_UTR 35.0%
AAGAAGAAGAGTTGCTGAAT+CGG - chr1_4:7782868-7782887 Msa0147750:three_prime_UTR 35.0%
TGTTGTTAATCATTGCTGTC+TGG - chr1_4:7783224-7783243 Msa0147750:intron 35.0%
TGAGTTGTAGAATTGAATGG+TGG - chr1_4:7783276-7783295 Msa0147750:intron 35.0%
! GCATCGACAATTCTAATTGA+AGG + chr1_4:7783392-7783411 None:intergenic 35.0%
! TGATTCAGTCCTTCATTTTC+AGG + chr1_4:7783445-7783464 None:intergenic 35.0%
! TCATTTTGTGAAAGGTTGTC+AGG - chr1_4:7783471-7783490 Msa0147750:intron 35.0%
! CATTTTGTGAAAGGTTGTCA+GGG - chr1_4:7783472-7783491 Msa0147750:intron 35.0%
TAAGTAACTGAGACATGGAT+AGG + chr1_4:7783518-7783537 None:intergenic 35.0%
AGTAGTAAGTAACTGAGACA+TGG + chr1_4:7783523-7783542 None:intergenic 35.0%
TATTAATGAATGTGGTGTCC+AGG + chr1_4:7783582-7783601 None:intergenic 35.0%
! ATCAATTCTGTCCAGTTTTG+GGG - chr1_4:7783753-7783772 Msa0147750:intron 35.0%
!! TTCTAATTCATCTCTAGAGC+AGG - chr1_4:7783820-7783839 Msa0147750:intron 35.0%
AGCGAGAAATAAAAGTCCTT+AGG + chr1_4:7784038-7784057 None:intergenic 35.0%
GTATAATCGAGTATCAACTG+TGG - chr1_4:7784093-7784112 Msa0147750:intron 35.0%
! TCTTCCTTTTACCTTCCAAA+AGG + chr1_4:7784119-7784138 None:intergenic 35.0%
TTAAAGGAAACGAAACTGAG+TGG + chr1_4:7784303-7784322 None:intergenic 35.0%
GCAAATCATCATAAGGAATG+TGG + chr1_4:7784483-7784502 None:intergenic 35.0%
!!! TCACACTTTTCAGTTTTGGA+AGG - chr1_4:7784807-7784826 Msa0147750:CDS 35.0%
GTTTCAGATTCGAATTCCAA+AGG + chr1_4:7782740-7782759 None:intergenic 35.0%
CTGAAACCAAAAACAGCAAA+CGG - chr1_4:7782753-7782772 Msa0147750:three_prime_UTR 35.0%
AAGAAGAAGAGTTGCTGAAT+CGG - chr1_4:7782868-7782887 Msa0147750:three_prime_UTR 35.0%
TGTTGTTAATCATTGCTGTC+TGG - chr1_4:7783224-7783243 Msa0147750:intron 35.0%
TGAGTTGTAGAATTGAATGG+TGG - chr1_4:7783276-7783295 Msa0147750:intron 35.0%
! GCATCGACAATTCTAATTGA+AGG + chr1_4:7783392-7783411 None:intergenic 35.0%
! TGATTCAGTCCTTCATTTTC+AGG + chr1_4:7783445-7783464 None:intergenic 35.0%
! TCATTTTGTGAAAGGTTGTC+AGG - chr1_4:7783471-7783490 Msa0147750:intron 35.0%
! CATTTTGTGAAAGGTTGTCA+GGG - chr1_4:7783472-7783491 Msa0147750:intron 35.0%
TAAGTAACTGAGACATGGAT+AGG + chr1_4:7783518-7783537 None:intergenic 35.0%
AGTAGTAAGTAACTGAGACA+TGG + chr1_4:7783523-7783542 None:intergenic 35.0%
TATTAATGAATGTGGTGTCC+AGG + chr1_4:7783582-7783601 None:intergenic 35.0%
! ATCAATTCTGTCCAGTTTTG+GGG - chr1_4:7783753-7783772 Msa0147750:intron 35.0%
!! TTCTAATTCATCTCTAGAGC+AGG - chr1_4:7783820-7783839 Msa0147750:intron 35.0%
AGCGAGAAATAAAAGTCCTT+AGG + chr1_4:7784038-7784057 None:intergenic 35.0%
GTATAATCGAGTATCAACTG+TGG - chr1_4:7784093-7784112 Msa0147750:intron 35.0%
! TCTTCCTTTTACCTTCCAAA+AGG + chr1_4:7784119-7784138 None:intergenic 35.0%
TTAAAGGAAACGAAACTGAG+TGG + chr1_4:7784303-7784322 None:intergenic 35.0%
GCAAATCATCATAAGGAATG+TGG + chr1_4:7784483-7784502 None:intergenic 35.0%
!!! TCACACTTTTCAGTTTTGGA+AGG - chr1_4:7784807-7784826 Msa0147750:CDS 35.0%
! GTGCTTCACGATTTTTCACA+TGG - chr1_4:7782946-7782965 Msa0147750:CDS 40.0%
! GTAAAGAGTTTGAGCTTGCT+AGG - chr1_4:7783018-7783037 Msa0147750:intron 40.0%
ATTGTCACTCGGTTGCTTAT+CGG - chr1_4:7783059-7783078 Msa0147750:intron 40.0%
CAACGCTATTGATAAGCTTG+CGG - chr1_4:7783100-7783119 Msa0147750:intron 40.0%
GTTGTAGAATTGAATGGTGG+TGG - chr1_4:7783279-7783298 Msa0147750:intron 40.0%
TACTTAGCTTCAAGGTTGTC+AGG - chr1_4:7783539-7783558 Msa0147750:intron 40.0%
CCAAAAGAATTCCCCAAAAC+TGG + chr1_4:7783767-7783786 None:intergenic 40.0%
!!! CCAGTTTTGGGGAATTCTTT+TGG - chr1_4:7783764-7783783 Msa0147750:intron 40.0%
TCTAAATCTTCATGTGTCGG+TGG + chr1_4:7784242-7784261 None:intergenic 40.0%
TCGTCTCCTAGTGATGAATA+AGG - chr1_4:7784344-7784363 Msa0147750:intron 40.0%
!! TTCCAGCCTTATTCATCACT+AGG + chr1_4:7784353-7784372 None:intergenic 40.0%
CATCATAAGGAATGTGGGTT+GGG + chr1_4:7784477-7784496 None:intergenic 40.0%
TCATCATAAGGAATGTGGGT+TGG + chr1_4:7784478-7784497 None:intergenic 40.0%
CCAGATCGCAAATCATCATA+AGG + chr1_4:7784490-7784509 None:intergenic 40.0%
! CCTTATGATGATTTGCGATC+TGG - chr1_4:7784487-7784506 Msa0147750:intron 40.0%
! GTCGAGTATTGTGGAATATG+TGG - chr1_4:7784855-7784874 Msa0147750:intron 40.0%
TCCACACTGATATCAATGTC+TGG - chr1_4:7785007-7785026 Msa0147750:CDS 40.0%
TCCAGACATTGATATCAGTG+TGG + chr1_4:7785011-7785030 None:intergenic 40.0%
! GTGCTTCACGATTTTTCACA+TGG - chr1_4:7782946-7782965 Msa0147750:CDS 40.0%
! GTAAAGAGTTTGAGCTTGCT+AGG - chr1_4:7783018-7783037 Msa0147750:intron 40.0%
ATTGTCACTCGGTTGCTTAT+CGG - chr1_4:7783059-7783078 Msa0147750:intron 40.0%
CAACGCTATTGATAAGCTTG+CGG - chr1_4:7783100-7783119 Msa0147750:intron 40.0%
GTTGTAGAATTGAATGGTGG+TGG - chr1_4:7783279-7783298 Msa0147750:intron 40.0%
TACTTAGCTTCAAGGTTGTC+AGG - chr1_4:7783539-7783558 Msa0147750:intron 40.0%
CCAAAAGAATTCCCCAAAAC+TGG + chr1_4:7783767-7783786 None:intergenic 40.0%
!!! CCAGTTTTGGGGAATTCTTT+TGG - chr1_4:7783764-7783783 Msa0147750:intron 40.0%
TCTAAATCTTCATGTGTCGG+TGG + chr1_4:7784242-7784261 None:intergenic 40.0%
TCGTCTCCTAGTGATGAATA+AGG - chr1_4:7784344-7784363 Msa0147750:intron 40.0%
!! TTCCAGCCTTATTCATCACT+AGG + chr1_4:7784353-7784372 None:intergenic 40.0%
CATCATAAGGAATGTGGGTT+GGG + chr1_4:7784477-7784496 None:intergenic 40.0%
TCATCATAAGGAATGTGGGT+TGG + chr1_4:7784478-7784497 None:intergenic 40.0%
CCAGATCGCAAATCATCATA+AGG + chr1_4:7784490-7784509 None:intergenic 40.0%
! CCTTATGATGATTTGCGATC+TGG - chr1_4:7784487-7784506 Msa0147750:intron 40.0%
! GTCGAGTATTGTGGAATATG+TGG - chr1_4:7784855-7784874 Msa0147750:intron 40.0%
TCCACACTGATATCAATGTC+TGG - chr1_4:7785007-7785026 Msa0147750:CDS 40.0%
TCCAGACATTGATATCAGTG+TGG + chr1_4:7785011-7785030 None:intergenic 40.0%
AAGAAGAGTTGCTGAATCGG+CGG - chr1_4:7782871-7782890 Msa0147750:three_prime_UTR 45.0%
TGTAGAGACTCAGTGCTTCT+CGG + chr1_4:7782914-7782933 None:intergenic 45.0%
!! TGAGCTTGCTAGGTTTGAGA+CGG - chr1_4:7783028-7783047 Msa0147750:intron 45.0%
!! AGAATTGAATGGTGGTGGTG+AGG - chr1_4:7783284-7783303 Msa0147750:intron 45.0%
ATGCTACAGAGTGCGAAACT+TGG - chr1_4:7783408-7783427 Msa0147750:intron 45.0%
! GACATCATGATGCCTTTTCC+TGG - chr1_4:7783561-7783580 Msa0147750:intron 45.0%
TGAATGTGGTGTCCAGGAAA+AGG + chr1_4:7783576-7783595 None:intergenic 45.0%
! GAGTATCAACTGTGGCCTTT+TGG - chr1_4:7784101-7784120 Msa0147750:intron 45.0%
! ATCAACTGTGGCCTTTTGGA+AGG - chr1_4:7784105-7784124 Msa0147750:intron 45.0%
!! GTGGCCTTTTGGAAGGTAAA+AGG - chr1_4:7784112-7784131 Msa0147750:intron 45.0%
CTCCTAGTGATGAATAAGGC+TGG - chr1_4:7784348-7784367 Msa0147750:intron 45.0%
TTGGAAGGACCTGTTCTTCA+AGG - chr1_4:7784822-7784841 Msa0147750:intron 45.0%
TTTGCCTCTCCTTGAAGAAC+AGG + chr1_4:7784834-7784853 None:intergenic 45.0%
AAGAAGAGTTGCTGAATCGG+CGG - chr1_4:7782871-7782890 Msa0147750:three_prime_UTR 45.0%
TGTAGAGACTCAGTGCTTCT+CGG + chr1_4:7782914-7782933 None:intergenic 45.0%
!! TGAGCTTGCTAGGTTTGAGA+CGG - chr1_4:7783028-7783047 Msa0147750:intron 45.0%
!! AGAATTGAATGGTGGTGGTG+AGG - chr1_4:7783284-7783303 Msa0147750:intron 45.0%
ATGCTACAGAGTGCGAAACT+TGG - chr1_4:7783408-7783427 Msa0147750:intron 45.0%
! GACATCATGATGCCTTTTCC+TGG - chr1_4:7783561-7783580 Msa0147750:intron 45.0%
TGAATGTGGTGTCCAGGAAA+AGG + chr1_4:7783576-7783595 None:intergenic 45.0%
! GAGTATCAACTGTGGCCTTT+TGG - chr1_4:7784101-7784120 Msa0147750:intron 45.0%
! ATCAACTGTGGCCTTTTGGA+AGG - chr1_4:7784105-7784124 Msa0147750:intron 45.0%
!! GTGGCCTTTTGGAAGGTAAA+AGG - chr1_4:7784112-7784131 Msa0147750:intron 45.0%
CTCCTAGTGATGAATAAGGC+TGG - chr1_4:7784348-7784367 Msa0147750:intron 45.0%
TTGGAAGGACCTGTTCTTCA+AGG - chr1_4:7784822-7784841 Msa0147750:intron 45.0%
TTTGCCTCTCCTTGAAGAAC+AGG + chr1_4:7784834-7784853 None:intergenic 45.0%
GAGGAAAATGGCGAAGCCTT+TGG - chr1_4:7782721-7782740 Msa0147750:three_prime_UTR 50.0%
!!! GCGCAACCGTTTGCTGTTTT+TGG + chr1_4:7782762-7782781 None:intergenic 50.0%
TTCACATGGCACGATGCCAA+AGG - chr1_4:7782960-7782979 Msa0147750:CDS 50.0%
!! ACGATGCCAAAGGCGTTCTA+TGG - chr1_4:7782970-7782989 Msa0147750:CDS 50.0%
ATCACGCCATAGAACGCCTT+TGG + chr1_4:7782979-7782998 None:intergenic 50.0%
! TGCTAGGTTTGAGACGGATC+CGG - chr1_4:7783034-7783053 Msa0147750:intron 50.0%
AGCAACCGAGTGACAATCTC+CGG + chr1_4:7783056-7783075 None:intergenic 50.0%
GCTTGCGGAACAGATTCAGA+AGG - chr1_4:7783115-7783134 Msa0147750:intron 50.0%
CAGATTCAGAAGGATCGTCG+CGG - chr1_4:7783125-7783144 Msa0147750:intron 50.0%
!! TGGACACCGACACTTGTTGT+TGG - chr1_4:7783361-7783380 Msa0147750:intron 50.0%
GCGTGTCCAACAACAAGTGT+CGG + chr1_4:7783370-7783389 None:intergenic 50.0%
AGGGAACATGTTAGGAACCC+AGG + chr1_4:7783799-7783818 None:intergenic 50.0%
AAGAGGGAAGCAGTACCATC+CGG + chr1_4:7783977-7783996 None:intergenic 50.0%
!! GTGGGTTGGGTTGATTCCAT+TGG + chr1_4:7784464-7784483 None:intergenic 50.0%
AGGACCTGTTCTTCAAGGAG+AGG - chr1_4:7784827-7784846 Msa0147750:intron 50.0%
! GAGGCAAACGTCGAGTATTG+TGG - chr1_4:7784846-7784865 Msa0147750:intron 50.0%
TATGTGGCCAAGTAAGCAGC+TGG - chr1_4:7784871-7784890 Msa0147750:CDS 50.0%
GAGGAAAATGGCGAAGCCTT+TGG - chr1_4:7782721-7782740 Msa0147750:three_prime_UTR 50.0%
!!! GCGCAACCGTTTGCTGTTTT+TGG + chr1_4:7782762-7782781 None:intergenic 50.0%
TTCACATGGCACGATGCCAA+AGG - chr1_4:7782960-7782979 Msa0147750:CDS 50.0%
!! ACGATGCCAAAGGCGTTCTA+TGG - chr1_4:7782970-7782989 Msa0147750:CDS 50.0%
ATCACGCCATAGAACGCCTT+TGG + chr1_4:7782979-7782998 None:intergenic 50.0%
! TGCTAGGTTTGAGACGGATC+CGG - chr1_4:7783034-7783053 Msa0147750:intron 50.0%
AGCAACCGAGTGACAATCTC+CGG + chr1_4:7783056-7783075 None:intergenic 50.0%
GCTTGCGGAACAGATTCAGA+AGG - chr1_4:7783115-7783134 Msa0147750:intron 50.0%
CAGATTCAGAAGGATCGTCG+CGG - chr1_4:7783125-7783144 Msa0147750:intron 50.0%
!! TGGACACCGACACTTGTTGT+TGG - chr1_4:7783361-7783380 Msa0147750:intron 50.0%
GCGTGTCCAACAACAAGTGT+CGG + chr1_4:7783370-7783389 None:intergenic 50.0%
AGGGAACATGTTAGGAACCC+AGG + chr1_4:7783799-7783818 None:intergenic 50.0%
AAGAGGGAAGCAGTACCATC+CGG + chr1_4:7783977-7783996 None:intergenic 50.0%
!! GTGGGTTGGGTTGATTCCAT+TGG + chr1_4:7784464-7784483 None:intergenic 50.0%
AGGACCTGTTCTTCAAGGAG+AGG - chr1_4:7784827-7784846 Msa0147750:intron 50.0%
! GAGGCAAACGTCGAGTATTG+TGG - chr1_4:7784846-7784865 Msa0147750:intron 50.0%
TATGTGGCCAAGTAAGCAGC+TGG - chr1_4:7784871-7784890 Msa0147750:CDS 50.0%
AAACAGCAAACGGTTGCGCG+TGG - chr1_4:7782763-7782782 Msa0147750:three_prime_UTR 55.0%
GCGTGGCGGTTATTACACGA+CGG - chr1_4:7782780-7782799 Msa0147750:three_prime_UTR 55.0%
AGAAGCTCTTCCACGGTGTC+AGG + chr1_4:7782807-7782826 None:intergenic 55.0%
TGACACCGTGGAAGAGCTTC+TGG - chr1_4:7782806-7782825 Msa0147750:three_prime_UTR 55.0%
CCGTTCCAGAAGCTCTTCCA+CGG + chr1_4:7782814-7782833 None:intergenic 55.0%
! CCGTGGAAGAGCTTCTGGAA+CGG - chr1_4:7782811-7782830 Msa0147750:three_prime_UTR 55.0%
CGGATCCGGAGATTGTCACT+CGG - chr1_4:7783048-7783067 Msa0147750:intron 55.0%
GTCACTCGGTTGCTTATCGG+TGG - chr1_4:7783062-7783081 Msa0147750:intron 55.0%
GTTGCTTATCGGTGGACGTG+AGG - chr1_4:7783070-7783089 Msa0147750:intron 55.0%
ATTCAGAAGGATCGTCGCGG+TGG - chr1_4:7783128-7783147 Msa0147750:intron 55.0%
CTCCTAAGCCCTGAGCTATG+CGG - chr1_4:7783929-7783948 Msa0147750:intron 55.0%
TAAGCCCTGAGCTATGCGGT+GGG - chr1_4:7783933-7783952 Msa0147750:intron 55.0%
AGAGGGAAGCAGTACCATCC+GGG + chr1_4:7783976-7783995 None:intergenic 55.0%
AGTGCAGCCAGCTGCTTACT+TGG + chr1_4:7784881-7784900 None:intergenic 55.0%
AAACAGCAAACGGTTGCGCG+TGG - chr1_4:7782763-7782782 Msa0147750:three_prime_UTR 55.0%
GCGTGGCGGTTATTACACGA+CGG - chr1_4:7782780-7782799 Msa0147750:three_prime_UTR 55.0%
AGAAGCTCTTCCACGGTGTC+AGG + chr1_4:7782807-7782826 None:intergenic 55.0%
TGACACCGTGGAAGAGCTTC+TGG - chr1_4:7782806-7782825 Msa0147750:three_prime_UTR 55.0%
CCGTTCCAGAAGCTCTTCCA+CGG + chr1_4:7782814-7782833 None:intergenic 55.0%
! CCGTGGAAGAGCTTCTGGAA+CGG - chr1_4:7782811-7782830 Msa0147750:three_prime_UTR 55.0%
CGGATCCGGAGATTGTCACT+CGG - chr1_4:7783048-7783067 Msa0147750:intron 55.0%
GTCACTCGGTTGCTTATCGG+TGG - chr1_4:7783062-7783081 Msa0147750:intron 55.0%
GTTGCTTATCGGTGGACGTG+AGG - chr1_4:7783070-7783089 Msa0147750:intron 55.0%
ATTCAGAAGGATCGTCGCGG+TGG - chr1_4:7783128-7783147 Msa0147750:intron 55.0%
CTCCTAAGCCCTGAGCTATG+CGG - chr1_4:7783929-7783948 Msa0147750:intron 55.0%
TAAGCCCTGAGCTATGCGGT+GGG - chr1_4:7783933-7783952 Msa0147750:intron 55.0%
AGAGGGAAGCAGTACCATCC+GGG + chr1_4:7783976-7783995 None:intergenic 55.0%
AGTGCAGCCAGCTGCTTACT+TGG + chr1_4:7784881-7784900 None:intergenic 55.0%
CACCGCATAGCTCAGGGCTT+AGG + chr1_4:7783934-7783953 None:intergenic 60.0%
CTAAGCCCTGAGCTATGCGG+TGG - chr1_4:7783932-7783951 Msa0147750:intron 60.0%
CAAGCCCACCGCATAGCTCA+GGG + chr1_4:7783940-7783959 None:intergenic 60.0%
ACAAGCCCACCGCATAGCTC+AGG + chr1_4:7783941-7783960 None:intergenic 60.0%
GCTTGTAAACCCCAGCCACC+CGG - chr1_4:7783955-7783974 Msa0147750:intron 60.0%
CACCGCATAGCTCAGGGCTT+AGG + chr1_4:7783934-7783953 None:intergenic 60.0%
CTAAGCCCTGAGCTATGCGG+TGG - chr1_4:7783932-7783951 Msa0147750:intron 60.0%
CAAGCCCACCGCATAGCTCA+GGG + chr1_4:7783940-7783959 None:intergenic 60.0%
ACAAGCCCACCGCATAGCTC+AGG + chr1_4:7783941-7783960 None:intergenic 60.0%
GCTTGTAAACCCCAGCCACC+CGG - chr1_4:7783955-7783974 Msa0147750:intron 60.0%
CAGCAAACGGTTGCGCGTGG+CGG - chr1_4:7782766-7782785 Msa0147750:three_prime_UTR 65.0%
ACACGACGGTCCTGACACCG+TGG - chr1_4:7782794-7782813 Msa0147750:three_prime_UTR 65.0%
AAGGATCGTCGCGGTGGCAG+TGG - chr1_4:7783134-7783153 Msa0147750:intron 65.0%
GTAAACCCCAGCCACCCGGA+TGG - chr1_4:7783959-7783978 Msa0147750:intron 65.0%
CAGTACCATCCGGGTGGCTG+GGG + chr1_4:7783967-7783986 None:intergenic 65.0%
GCAGTACCATCCGGGTGGCT+GGG + chr1_4:7783968-7783987 None:intergenic 65.0%
AGCAGTACCATCCGGGTGGC+TGG + chr1_4:7783969-7783988 None:intergenic 65.0%
GGGAAGCAGTACCATCCGGG+TGG + chr1_4:7783973-7783992 None:intergenic 65.0%
CAGCAAACGGTTGCGCGTGG+CGG - chr1_4:7782766-7782785 Msa0147750:three_prime_UTR 65.0%
ACACGACGGTCCTGACACCG+TGG - chr1_4:7782794-7782813 Msa0147750:three_prime_UTR 65.0%
AAGGATCGTCGCGGTGGCAG+TGG - chr1_4:7783134-7783153 Msa0147750:intron 65.0%
GTAAACCCCAGCCACCCGGA+TGG - chr1_4:7783959-7783978 Msa0147750:intron 65.0%
CAGTACCATCCGGGTGGCTG+GGG + chr1_4:7783967-7783986 None:intergenic 65.0%
GCAGTACCATCCGGGTGGCT+GGG + chr1_4:7783968-7783987 None:intergenic 65.0%
AGCAGTACCATCCGGGTGGC+TGG + chr1_4:7783969-7783988 None:intergenic 65.0%
GGGAAGCAGTACCATCCGGG+TGG + chr1_4:7783973-7783992 None:intergenic 65.0%
CAGTGCTTCTCGGCGCGTGC+TGG + chr1_4:7782904-7782923 None:intergenic 70.0%
GATCGTCGCGGTGGCAGTGG+CGG - chr1_4:7783137-7783156 Msa0147750:intron 70.0%
CAGTGCTTCTCGGCGCGTGC+TGG + chr1_4:7782904-7782923 None:intergenic 70.0%
GATCGTCGCGGTGGCAGTGG+CGG - chr1_4:7783137-7783156 Msa0147750:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 7782717 7785096 7782717 ID=Msa0147750;Name=Msa0147750
chr1_4 mRNA 7782717 7785096 7782717 ID=Msa0147750-mRNA-1;Parent=Msa0147750;Name=Msa0147750-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0.33|1|0|0.33|3|179|167
chr1_4 exon 7782717 7782993 7782717 ID=Msa0147750-mRNA-1:exon:3349;Parent=Msa0147750-mRNA-1
chr1_4 exon 7784655 7784831 7784655 ID=Msa0147750-mRNA-1:exon:3348;Parent=Msa0147750-mRNA-1
chr1_4 exon 7784868 7785096 7784868 ID=Msa0147750-mRNA-1:exon:3347;Parent=Msa0147750-mRNA-1
chr1_4 CDS 7784868 7785096 7784868 ID=Msa0147750-mRNA-1:cds;Parent=Msa0147750-mRNA-1
chr1_4 CDS 7784655 7784831 7784655 ID=Msa0147750-mRNA-1:cds;Parent=Msa0147750-mRNA-1
chr1_4 CDS 7782896 7782993 7782896 ID=Msa0147750-mRNA-1:cds;Parent=Msa0147750-mRNA-1
chr1_4 three_prime_UTR 7782717 7782895 7782717 ID=Msa0147750-mRNA-1:three_prime_utr;Parent=Msa0147750-mRNA-1
Gene Sequence

>Msa0147750

ATGAGAGGAAAATGGCGAAGCCTTTGGAATTCGAATCTGAAACCAAAAACAGCAAACGGTTGCGCGTGGCGGTTATTACACGACGGTCCTGACACCGTGGAAGAGCTTCTGGAACGGCATCTAGTGAAGAAAGTTATCAACGATGATGAAGAAGAAGAAGAGTTGCTGAATCGGCGGCGACTCACCAGCACGCGCCGAGAAGCACTGAGTCTCTACAGAGACATTCTTCGCGTTCTATGGCGTGATCTTCTTAGAGATAGCGCTCGTAAAGAGTTTGAGCTTGCTAGGTTTGAGACGGATCCGGAGATTGTCACTCGGTTGCTTATCGGTGGACGTGAGGCTGTCAACAACGCTATTGATAAGCTTGCGGAACAGATTCAGAAGGATCGTCGCGGTGGCAGTGGCGTTTTGGAAGGACCTGTTCTTCAAGGAGAGGCAAACGTCGAGTATTGTGGAATATGTGGCCAAGTAAGCAGCTGGCTGCACTGCAATACTATCTTGTAG
Protein sequence

>Msa0147750

MRGKWRSLWNSNLKPKTANGCAWRLLHDGPDTVEELLERHLVKKVINDDEEEEELLNRRRLTSTRREALSLYRDILRVLWRDLLRDSARKEFELARFETDPEIVTRLLIGGREAVNNAIDKLAEQIQKDRRGGSGVLEGPVLQGEANVEYCGICGQVSSWLHCNTIL*